CN101168491B - Microorganism soil repairing agent and its preparation method and application - Google Patents
Microorganism soil repairing agent and its preparation method and application Download PDFInfo
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Abstract
The invention relates to a microbe soil repairing additive and process for preparation. The process for preparation comprises that liquid microbe fertilizer containing photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria are absorbed to one or a plurality of natural organic fertilizers as grass peat and zeolite powder or the like, to be mixed proportionally. The invention further relates to the application of the microbe soil repairing additive for repairing soil. The invention can lead useful microbe into soil, uses microbe technique to transfer, absorb, degrade, and convert the contaminant in soil, adjust soil pH value, eliminate soil blocking, and restrain soil-borne disease, thereby improving soil structure, to return soil to original ecologic structure. And the viable bacteria group of the invention is planted in plant root, to quickly grow and deeply insert plant root, thereby improving yield, quality and level of agricultural product, as one ideal fertilizer of organic agriculture, with wide application in agriculture continuous development.
Description
Technical field
The invention belongs to fertilizer field, be specifically related to a kind of microbe soil restoration agent.
Background technology
Along with the development of industrial and agricultural production, particularly the edatope pollution problem is more and more outstanding for agricultural pollution in China! The arable land that the whole nation receives heavy metal contamination is nearly more than 2,000 ten thousand hectares, receives about more than 6,000 ten thousand hectares of the farmland of agricultural chemicals and other Pollution by Chemicals.Soil environment quality is directly connected to the safety of agricultural-food.Since the soil pollution in wide area, nearly 1,200 ten thousand tons in the grain of the annual production heavy metal contamination of China; In the main farm produce that the whole nation produces, the pesticide residue exceeding standard rate is up to 16～20%, and problem is very serious, and agricultural products in China " basically " does not have safety control! In many major areas, soil and groundwater pollution have caused the M & M of diseases such as cancer apparently higher than many times of the check plot several times to 10 that do not have to pollute.Can not doubt, behind the entering WTO, soil pollution will become one of restriction agricultural products in China international trade and social economy's Sustainable development major obstacles, press for reparation, administer.
In the world, soil science research develops into environmental edaphology from traditional agricultural soil science, and the research of contaminated soil reparation has become the subject forward position of soil science.Carry out the research and the applied research of contaminated soil generating process and regulation and control, contaminated soil reparation in a deep going way, tightly grasp the direction of contaminated soil recovery technique innovation, be directly connected to China's agricultural pollution and ecological security.
Existing various contaminated soil recovery technique; Because the restriction of certain scope of application is all arranged, and some problem of existence more or less, wherein some or even the technological difficulties that are difficult to overcome; How to solve these technological difficulties; How walking out the predicament and the mistaken ideas of present research, how to reproduce in the innovation and the technology of carrying out on the whole meaning from technological concept under the prerequisite of ecological security, is a current big science difficult problem that presses for solution.
Microbe soil restoration agent among the present invention is meant that natural organic fertilizers such as utilizing the peat composed of rotten mosses, zeolite powder makes carrier the multiple beneficial microbial composite bacteria is brought in the soil; Through the pollutent in microbial technique transfer, absorption, degraded and the conversion soil; Make its concentration be reduced to acceptable level; Or poisonous and hazardous pollutent is converted into harmless material, thus reach the ecosystem Soil structure, solved soil and problem of environmental pollution from root; Solve the safety-problems of agricultural-food, really realized human beings'health.
Summary of the invention
The purpose of this invention is to provide a kind of microbe soil restoration agent.
The present invention also aims to provide the preparation method of this microbe soil restoration agent.
The present invention also aims to provide the application of this microbe soil restoration agent aspect rehabilitating soil.
The present invention also aims to provide the application of this microbe soil restoration agent aspect plant husbandry such as paddy rice, wheat, cotton, tobacco, vegetables, melon and fruit.
The present invention also aims to use this microbe soil restoration agent can reduce the usage quantity of chemical fertilizer and agricultural chemicals, improve the security of agricultural-food.
The invention provides a kind of microbe soil restoration agent, contain photosynthetic bacterium, genus bacillus, actinomycetes and milk-acid bacteria, its natural organic fertilizers such as absorption peat composed of rotten mosses, zeolite powder are made carrier stir in proportion and form by a kind of liquid microbe fertilizer.
Wherein, the volume of said each zymocyte liquid by volume part meter be preferably: actinomycetes fermentation liquor 1～10, photosynthetic bacterium fermented liquid 1～10, fermentation of bacillus liquid 1～20, streptococcus acidi lactici fermented solution 1～10.
The unit living bacteria count is not less than 500,000,000/milliliter in the various zymocyte liquids.
The present invention also provides the preparation method of said microbe soil restoration agent.This preparation method comprises the steps:
(1) respectively the mother of photosynthetic bacterium, genus bacillus, actinomycetes and milk-acid bacteria is planted and be inoculated in the one-level substratum and cultivate, obtain first class inoculum;
(2) respectively photosynthetic bacterium, genus bacillus, actinomycetic first class inoculum are inoculated in secondary medium and cultivate, obtain second class inoculum;
(3) respectively photosynthetic bacterium, genus bacillus, actinomycetic second class inoculum are inoculated in fermention medium and cultivate, obtain zymocyte liquid;
(4) with each zymocyte liquid in proportion mixing promptly get liquid microbe fertilizer;
The difference sprinkling amount of (5) microbial fertilizer being pressed absorption carrier (natural organic fertilizers such as the peat composed of rotten mosses, zeolite powder) is 20%～40%, and humidity remains on about 35%, mixes with carrier to stir promptly to get.
Inoculum size was preferably 20%～50% when the photosynthetic bacterium one-level was cultivated in the said step (1), cultivated pH and was preferably 6～10, and culture temperature is preferably 28～36 ℃, and intensity of illumination is preferably 3000～4000 luxs, and incubation time is preferably 7～10 days; Inoculum size was preferably 5%～15% when the genus bacillus one-level was cultivated, and cultivated pH and was preferably 6～8, and culture temperature is preferably 28～34 ℃, and incubation time is preferably 2～3 days; Inoculum size was preferably 10%～50% when the actinomycetes one-level was cultivated, and cultivated pH and was preferably 6～8, and culture temperature is preferably 27～32 ℃, and incubation time is preferably 5～7 days; Inoculum size was preferably 5%～20% when the milk-acid bacteria one-level was cultivated, and cultivated pH and was preferably 6～8, and culture temperature is preferably 28～34 ℃, and incubation time is preferably 2～5.
The photosynthetic bacterium secondary is cultivated and is preferably the intermittent type aerobic culture in the said step (2); Inoculum size is preferably 20%～50%, cultivates pH and is preferably 6～10, and culture temperature is preferably 28～36 ℃; Intensity of illumination is preferably 3000～4000 luxs, and incubation time is preferably 7～10 days; The genus bacillus secondary is cultivated and is preferably aerobic culture, and inoculum size is preferably 5%～15%, cultivates pH and is preferably 6～8, and culture temperature is preferably 28～32 ℃, and incubation time is preferably 2～5 days; The actinomycetes secondary is cultivated and is preferably aerobic culture, and inoculum size is preferably 20%～50%, cultivates pH and is preferably 6～8, and culture temperature is preferably 27～32 ℃, and incubation time is preferably 5～7 days; The milk-acid bacteria secondary is cultivated and is preferably the stuffiness cultivation, and inoculum size is preferably 5%～20%, cultivates pH and is preferably 6～8, and culture temperature is preferably 28～34 ℃, and incubation time is preferably 2～5 days.
The photosynthetic bacterium fermentation culture is preferably the intermittent type aerobic culture in the said step (3), and second class inoculum is inoculated in the 500L seeding tank, and inoculum size is preferably 20%～50%; Cultivate pH and be preferably 7～8, culture temperature is preferably 28～36 ℃, and intensity of illumination is preferably 3000～4000 luxs; Incubation time is preferably 7～10 days, is inoculated in 2 tons of fermentor tanks afterwards, and inoculum size is preferably 20%～50%; Cultivate pH and be preferably 7～8; Culture temperature is preferably 28～36 ℃, and intensity of illumination is preferably 3000～4000 luxs, and incubation time is preferably 7～10 days; Fermentation of bacillus is cultivated and is preferably aerobic culture, and second class inoculum is inoculated in the 200L seeding tank, and inoculum size is preferably 5%～15%; Cultivate pH and be preferably 6～8, culture temperature is preferably 28～32 ℃, and incubation time is preferably 2～3 days; Be inoculated in 2 tons of fermentor tanks afterwards, inoculum size is preferably 20%～50%, cultivates pH and is preferably 7～8; Culture temperature is preferably 28～36 ℃, and incubation time is preferably 2～3 days; Actinomycete fermentation is cultivated and is preferably aerobic culture, and second class inoculum is inoculated in the 500mL seeding tank, and inoculum size is preferably 20%～50%; Cultivate pH and be preferably 6～8, culture temperature is preferably 27～32 ℃, is inoculated in 2 tons of fermentor tanks after incubation time is preferably 5～7 days; Inoculum size is preferably 20%～50%; It is preferred 7～8 to cultivate pH, and culture temperature is preferably 28～36 ℃, and incubation time is preferably 7～10 days; Lactobacillus-fermented is cultivated to stuffiness and is cultivated, and second class inoculum is inoculated in the 200L seeding tank, and inoculum size is preferably 5%～20%; Cultivate pH and be preferably 6～8, culture temperature is preferably 28～32 ℃, and incubation time is preferably 2～3 days; Be inoculated in 2 tons of fermentor tanks afterwards, inoculum size is preferably 5%～20%, cultivates pH and is preferably 7～8; Culture temperature is preferably 28～36 ℃, and incubation time is preferably 2～3 days.
Said step (5) absorption carrier can be in the natural organic fertilizers such as the peat composed of rotten mosses, zeolite powder wherein one or more mixture.
The present invention also provides the application of said microbe soil restoration agent aspect rehabilitating soil.
The present invention also provides said microbe soil restoration agent to be applied in the application of plant husbandry aspects such as paddy rice, wheat, cotton, tobacco, vegetables, melon and fruit.
The present invention also provides the method for use of said microbe soil restoration agent, it is characterized in that mixing use, usage quantity first with base manure, composite fertilizer: 2～3kg/ mu, later annual maintenance: 1～2kg/ mu.
The present invention also provides said microbe soil restoration agent itself to contain the mass efficient viable bacteria, just can be absorbed by crop as nutrient in being manured into soil, and can not form residue in the ground.A series of microbial activitiess such as the growth through bacterial classification itself, breeding, death simultaneously are that soil is created soil ulmin, increase the organic content in the soil, the fertility of raising soil.These a series of microbial activitiess also can produce a large amount of " phosphoglycans "; Use curing and calcified chemical substance that chemical fertilizer caused residual owing to a large amount of for a long time in the middle of helping to decompose soil; And making the soil become loose, oxygen G&W etc. is able to circulation, makes the soil restoration vigour and vitality in the past that has hardened; For the growth of plant root creates loose environment, make crop root ramp, the dark bundle.Make that being deposited in inorganic nitrogen in the soil, phosphorus, potassium and trace element etc. all the year round is able to absorbed by plant root fully; Simultaneously; Make the unsound soil that is in morbid state be able to get well, recover power of wound certainly and the reproducibility of self, make it return to the Soil structure of ecosystem.The result that brought of these effects makes agricultural output high, and quality is good, quality better, beautiful outline form, and keeping quality is strong, and the fertility of soil is able to protection and strengthens.This efficient function is that other fertilizer is unexistent, and this microbe soil restoration agent is used in perhaps harden on thin and weak ground ground, the serious soil of disease and pest, and effect is more obvious.
Useful technique effect embodies as follows:
Use behind the present invention to farm crop provide growth necessary various nutritive elements, and it is active to increase in the soil beneficial microorganism, has overcome the excessive drawback that causes with uneven fertilising of using of chemical fertilizer simultaneously.The excessive chemical fertilizer of using can damage natural, ecological, like the increase of nitrate nitrogen content in the environment, and the secondary salinization on protection ground, the destruction of some Soil structure to the influence of vegetables, fruit quality, is caused the reduction and the Soil degradation of chemical fertilizer rate of returns at last.
The important task that soil organism soil ulmin transforms is being undertaken in this invention again, increases soil aggregate, improves the preserve moisture and fertility ability; The nutrient that activation has been cured by the soil improves chemical fertilizer utilization ratio, and the useful microbe in the microbe soil restoration agent is secreted various benefit materials to soil simultaneously; Growth stimulant, indolylacetic acid, Plant hormones regulators,gibberellins and various enzyme; Thereby promote the conversion of nutrient effectively, alleviated the generation of soil-borne disease, repaired Contaminated soil; Be the desirable fertilizer of green agriculture, Organic farming, bright prospects are arranged in agricultural sustainable development.
Description of drawings
Fig. 1 microbe soil restoration agent technological process of production figure.
Use the flue-cured tobacco rhizome contrast of flue-cured tobacco and the conventional fertilizer application of microbe soil restoration agent of the present invention in the soil of the equal fertility of Fig. 2, the demonstration group is more sturdy than the cane of control group, and fibrous root is many and grow.
Use the flue-cured tobacco contrast in vegetative period of the flue-cured tobacco and the conventional fertilizer application of microbe soil restoration agent of the present invention in the soil of the equal fertility of Fig. 3, the demonstration group is generouser than the blade of control group, the leaf look green, growing way is healthy.
Use the cotton of microbe soil restoration agent of the present invention and the cotton cane contrast of conventional fertilizer application in the soil of the equal fertility of Fig. 4, the demonstration comparison is sturdy according to cane, well developed root system, and the capillary root is many.
Use the cotton of microbe soil restoration agent of the present invention and the cotton growing stage contrast of conventional fertilizer application in the soil of the equal fertility of Fig. 5, the demonstration comparison is buddingged early according to flourishing, and cotton buds and bolls are many.
Use the paddy rice of microbe soil restoration agent of the present invention and the rice seedling root system contrast of conventional fertilizer application in the soil of the equal fertility of Fig. 6, the demonstration comparison is according to well developed root system, and the capillary root is many.
Use the paddy rice of microbe soil restoration agent of the present invention and the rice seedling contrast of conventional fertilizer application in the soil of the equal fertility of Fig. 7, the demonstration comparison is high according to percentage of germination, and seedling is neat, and seedling is strong, and the leaf look just green.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Wherein, photosynthetic bacterium one-level substratum is: MgSO47H2O 0.2g, (NH4) 2SO41.0g, NaHCO3 5.0g, K2HPO4 0.5g, NaCl 0.2g, peptone 1.5g; Each composition is dissolved in the 400ml zero(ppm) water, transfers pH7.1, add zero(ppm) water again, sterilized 10 minutes for 115 ℃ to 1000ml with H3PO4 and Na2CO3.Secondary medium is: sodium-acetate 3g, NaCl 0.1g, (NH4) 2SO4 0.3g, MgSO4 0.2g, KH2PO3 0.5g, K2HPO3 0.3g, ferric ammonium citrate 7ml, peptone 1.2g, zero(ppm) water are settled to 1000ml, and using H3PO4 and Na2CO3 to transfer pH is 7.2.Fermention medium is: sodium-acetate 2.6g, NaCl 0.07g, (NH4) 2SO40.25g, MgSO4 0.16g, KH2PO3 0.38g, K2HPO3 0.25g, ferric ammonium citrate 5ml, peptone 1.0g, water are settled to 1000ml, use H3PO4 and Na2CO3 to transfer pH is 7.1～7.2.
Bacillus one-level substratum is: Carnis Bovis seu Bubali cream 0.3%, peptone 1.0%, sodium-chlor 0.5%, zero(ppm) water are settled to 1000ml, pH7.0.Secondary medium is: Carnis Bovis seu Bubali cream 1.0%, peptone 1.0%, sodium-chlor 0.5%, agar 2.0%, zero(ppm) water are settled to 1000ml, pH7.0.Fermention medium is: starch 0.3%, sucrose 0.5%, 30.8mg/Kg manganese sulfate solution 0.2%, potassium hydrogenphosphate 0.3%, potassium primary phosphate 0.15%, dregs of beans 3.0%, water supplies 100%, pH7.0.
Actinomycetes one-level substratum is: saltpetre 1 gram, and Zulkovsky starch 20 grams, potassium hydrogenphosphate 0.5 gram, sal epsom 0.5 gram, sodium-chlor 0.5 gram, ferrous sulfate 0.01 gram, agar 20 grams, zero(ppm) water is supplied 1000ml, pH7.2～7.4.Secondary medium is saltpetre 0.8 gram, Zulkovsky starch 8 grams, and glucose 5 grams, yeast water 5 grams, potassium hydrogenphosphate 0.3 gram, sal epsom 0.2 gram, sodium-chlor 0.4 gram, ferrous sulfate 0.05 gram, water is supplied 1000ml, pH7.2～7.4.Fermention medium is: glucose 1%, and yeast water 1%, potassium hydrogenphosphate 0.015%, sodium-chlor 0.02%, ferrous sulfate 0.003%, water supplies 100%, pH7.2～7.5.
Milk-acid bacteria one-level substratum is: saltpetre 1 gram, and Zulkovsky starch 10 grams, potassium hydrogenphosphate 0.5 gram, sal epsom 0.5 gram, sodium-chlor 0.5 gram, ferrous sulfate 0.01 gram, agar 20 grams, zero(ppm) water is supplied 1000ml, pH7.2～7.4.Secondary medium is: saltpetre 0.8 gram, and Zulkovsky starch 8 grams, glucose 5 grams, yeast water 5 grams, potassium hydrogenphosphate 0.3 gram, sal epsom 0.2 gram, sodium-chlor 0.4 gram, ferrous sulfate 0.05 gram, water is supplied 1000ml, pH7.2～7.4.Fermention medium is: glucose 1%, and yeast water 1%, potassium hydrogenphosphate 0.015%, sodium-chlor 0.02%, ferrous sulfate 0.003%, water supplies 100%, pH7.2～7.5.
Embodiment 1
The working condition of each zymocyte liquid is seen table 1.
Wherein, intensity of illumination is 3000 luxs in the photosynthetic bacterium swamp Rhodopseudomonas culturing process.
Table 1
The culturing process bacterial classification
Swamp Rhodopseudomonas subtilis Jingyang streptomycete Lactobacterium acidophilum
One inoculum size (%) 20 15 10 5
Level pH 6786
The training temperature (℃) 32 28 32 28
Foster incubation time (my god) 7353
Inoculum size (%) 35 5 20 20
Secondary pH 8687
Culture temperature (℃) 28 30 32 28
Incubation time (my god) 7252
Seed inoculum size (%) 20 15 50 5
Jar training pH 7786
Foster temperature (℃) 28 28 32 28
Incubation time (my god) 7353
Fermentation inoculation (%) 50 20 20 12
Jar training pH 8787
Foster temperature (℃) 36 28 32 32
Incubation time (my god) 7373
Get 150 liters of the long-pending part meter swamp Rhodopseudomonas by volume of four kinds of that ferment and bacteria liquids that be up to the standards; 150 liters of subtilises, 700 liters of Jingyang streptomycetes, 150 liters of Lactobacterium acidophilums; Squeeze into container for storing liquid, be liquid microbe fertilizer after the bacterium liquid of container for storing liquid is mixed.
Embodiment 2
The working condition of each zymocyte liquid is seen table 2.
Wherein, intensity of illumination is 4000 luxs in the red microbial culture process of photosynthetic bacterium class ball.
Table 2
The culturing process bacterial classification
The red bacterium Bacillus cereus of class ball Jingyang streptomycete plant lactobacillus
One inoculum size (%) 50 5 50 12
Level pH 10 667
The training temperature (℃) 28 31 27 34
Foster incubation time (my god) 10 265
Inoculum size (%) 20 15 50 5
Secondary pH 6876
Culture temperature (℃) 36 28 27 34
Incubation time (my god) 10 565
Seed inoculum size (%) 50 5 20 20
Jar training pH 8667
Foster temperature (℃) 36 32 27 30
Incubation time (my god) 10 262
Fermentation inoculum size (%) 20 50 50 5
Jar training pH 7878
Temperature (℃) 28 36 28 28
Support
Incubation time (my god) 10 2 10 2
Get 100 liters of the long-pending part meter swamp Rhodopseudomonas by volume of four kinds of that ferment and bacteria liquids that be up to the standards; 100 liters of subtilises, 2000 liters of Jingyang streptomycetes, 100 liters of Lactobacterium acidophilums; Squeeze into container for storing liquid, be liquid microbe fertilizer after the bacterium liquid of container for storing liquid is mixed.
Embodiment 3
The working condition of each zymocyte liquid is seen table 3.
Wherein, intensity of illumination is 3500 luxs in the photosynthetic bacterium swamp Rhodopseudomonas culturing process.
Table 3
The culturing process bacterial classification
Swamp Rhodopseudomonas colloid bacillus cereus Jingyang streptomycete Lactobacterium acidophilum
One inoculum size (%) 35 8 30 20
Level pH 8878
The training temperature (℃) 36 34 30 31
Foster incubation time (my god) 9372
Inoculum size (%) 50 10 35 12
Secondary pH 10 768
Culture temperature (℃) 32 32 32 31
Incubation time (my god) 9353
Inoculum size (%) 35 10 35 12
Seed
Jar training pH 7878
Temperature (℃) 32 30 29 32
Support
Incubation time (my god) 8373
Inoculum size (%) 50 35 35 20
Fermentation
Jar training pH 8787
Temperature (℃) 32 32 36 36
Support
Incubation time (my god) 8393
Get 1000 liters of the long-pending part meter swamp Rhodopseudomonas by volume of four kinds of that ferment and bacteria liquids that be up to the standards; 1000 liters of subtilises, 100 liters of Jingyang streptomycetes, 1000 liters of Lactobacterium acidophilums; Squeeze into container for storing liquid, be liquid microbe fertilizer after the bacterium liquid of container for storing liquid is mixed.
Embodiment 4
The fermented liquid of microbial fertilizer is controlled at 20%～40% by the difference sprinkling amount of absorption carrier (natural organic fertilizers such as the peat composed of rotten mosses, zeolite powder), and humidity is controlled at about 35%, mixes with carrier to stir to get product.
Embodiment 5
The present invention further discloses said microbe soil restoration agent in rehabilitating soil method of use and be applied in the positive effect in the plant husbandry such as paddy rice, wheat, cotton, tobacco, vegetables, melon and fruit.
Microbe soil restoration agent of the present invention mixes use, usage quantity first with base manure, composite fertilizer: 2～3kg/ mu, later annual maintenance: 1～2kg/ mu.Impose in the soil, increase the organic content in the soil, improve the fertility of soil; Reach potassium decomposing, release phosphorus, the purpose of fixed nitrogen, the soil that hardens is improved rapidly; Make unsound power of wound certainly and the reproducibility that is in the soil restoration self of morbid state, make it return to the Soil structure of ecosystem.Be applied in the plant husbandry such as paddy rice, wheat, cotton, tobacco, vegetables, melon and fruit; Can reduce the consumption of chemical fertilizer 30～50%, reduce the consumption of agricultural chemicals, make crop root ramp, the dark bundle; Improve output, quality, the quality of agricultural-food, make it reach the standard of organic farm products.
Experimental example 1:
In late March, 2005 in Guizhou He Ba township, white agriculture demonstration county Fenggang County, town, honeybee rock town evolve; Carried out the demonstration work of this microbe soil restoration agent on flue-cured tobacco respectively; 170 mu of demonstration areas, edaphic condition, liquid manure and each item level of management are consistent in the demonstration program.Can find that according to following average data this microbe soil restoration agent can effectively improve cigarette ground soil physico-chemical property, suppress harmful microbe and grow procreation, improve crumb structure; Decompose organic matter, mineral substance in the soil, strengthen the tobacco plant disease resistance, reduce the consumption of chemical fertilizer, agricultural chemicals; Make crop root prosperities such as flue-cured tobacco, growing way is vigorous, and cane is sturdy, and no disease and pest improves quality of tobacco, improves yield of tobacco, reaches the standard of ecological high-quality cigarette.(like Fig. 2, Fig. 3)
(1) different growing stages performance:
1. seedling phase:
| Root | Leaf | Stem | Comprehensive growing way | |
| The demonstration group | Seedling too rootlet changes not obvious | Maximum leaf is wide slightly, thick slightly, the leaf look just green, number of blade 4-6 sheet | Short and strong | Neatly, seedling is neat, seedling is strong, growing way is fast |
| Control group | Not obvious | The narrower length of maximum leaf, thin slightly, the leaf look yellow, number of blade 3-5 sheet | Slight of stature | The weak seedling of irregular, indivedual appearance, growing way are slow slightly |
2. roll into a ball a phase: unit: cm, sheet
| Plant height | Leaf is long | Leaf is wide | Stem is thick | The number of blade | |
| The demonstration group | 91.20 | 64.99 | 26.59 | 10.55 | 23.60 |
| Control group | 79.20 | 63.51 | 24.96 | 9.93 | 22.80 |
| Gradient (demonstration: contrast) | 15.15％ | 2.33％ | 6.53％ | 6.24％ | 3.51％ |
(2) baking of flue-cured tobacco record unit: jin
(3) production benefit contrast unit: jin/mu
| Group | Quality | Output | Raising the output | Stimulation ratio |
| Demonstration | 80% is orange | 318.62 | 38.61 | 13.79％ |
| Contrast | 60% is orange | 280.01 |
Experimental example 2:
Shandong Zhanhua demonstration county Feng Jia town peasant Zhao Lianqing contracts ten mu of cotton fields in the east in the village; On the cotton field of contracting in 1986, planted the recent two decades cotton, cultivated meticulously every year, manage and protect meticulously, dropped into that substantial contribution is bought chemical fertilizer, agricultural chemicals is used to increase the land fertility and the insect pest of curing the disease of going out; But growth, the hardening soil along with implantation time; Disease and pest is rampant, constantly increases, manages and protects in fund input under the situation that energy do not subtract, and output of cotton but descends year by year.Particularly blight, the acces pernicieux of verticillium big area in recent years makes 70% cotton seedling death, and surviving rate only remains on about 30%, and the cotton plant height is paced up and down between 50～70 centimeters all the time, thereby causes output of cotton to descend significantly.In April, 2006, after Zhao Lianqing had used this invention, surprising variation has appearred in the cotton field: cotton shoot survival percent reached more than 98%; Cotton is flourishing; Cotton plant generally increases more than 30 centimeters, and cotton boll is individual greatly, the many 6-7 of Fu Qiantao are individual, cotton fiber length, soft smooth, pure white; Basically can't see cotton wilt, verticillium, insect pest, quality obviously improves; And cotton boll is full, and quantity is many, has 30 in the average strain, than usefulness not many one times, output improves more than 40%.(like Fig. 4, Fig. 5)
Claims (8)
1. a microbe soil restoration agent is characterized in that, the fermented liquid proportional mixing of photosynthetic bacterium, genus bacillus, actinomycetes and milk-acid bacteria is processed a kind of liquid microbe fertilizer; Described liquid microbe fertilizer and the stirring of absorption carrier proportional mixing are formed, and wherein, described absorption carrier is the peat composed of rotten mosses or zeolite powder; The volume of said each fermented liquid by volume part is counted: actinomycetes fermentation liquor 1～10; Photosynthetic bacterium fermented liquid 1～10, fermentation of bacillus liquid 1～20, streptococcus acidi lactici fermented solution 1～10; The ratio of said microbial fertilizer and absorption carrier: microbial fertilizer is pressed the different sprinkling amounts of absorption carrier 20%～40%, and carrier is 60%～80%; Wherein said photosynthetic bacterium is swamp Rhodopseudomonas (Rhodopseudmonas Palustris) or type red bacterium of ball (Rhodobactersphaeroides); Said genus bacillus is subtilis (Bacillus subtilis), Bacillus cereus (Bacillus cereus) or colloid bacillus cereus (Bacillus mucilaginosus); Said actinomycetes are Jingyang streptomycete (StreptomycesJing-yangensis); Said milk-acid bacteria is plant lactobacillus (Lactobacillus plantarum) or Lactobacterium acidophilum (Lactobacillus acidophilus).
2. the preparation method of the described microbe soil restoration agent of claim 1, this method comprises the steps:
(1) respectively the mother of photosynthetic bacterium, genus bacillus, actinomycetes and milk-acid bacteria is planted and be inoculated in the one-level substratum and cultivate, obtain first class inoculum;
(2) respectively above-mentioned first class inoculum is inoculated in secondary medium and cultivates, obtain second class inoculum;
(3) respectively above-mentioned second class inoculum is inoculated in fermention medium and cultivates, obtain zymocyte liquid;
(4) with each zymocyte liquid in proportion mixing promptly get liquid microbe fertilizer, the volume of said each fermented liquid by volume part is counted: actinomycetes fermentation liquor 1～10, photosynthetic bacterium fermented liquid 1～10, fermentation of bacillus liquid 1～20, streptococcus acidi lactici fermented solution 1～10;
The difference sprinkling amount of (5) microbial fertilizer being pressed absorption carrier is 20%～40%, and humidity is 35%, mixes to stir promptly getting with carrier.
3. the preparation method of microbe soil restoration agent according to claim 2 is characterized in that, inoculum size 20%～50% when the photosynthetic bacterium one-level is cultivated in the said step (1); Cultivate pH6～10; 28～36 ℃ of culture temperature, intensity of illumination 3000～4000 luxs, incubation time 7～10 days; Inoculum size 5%～15% when the genus bacillus one-level is cultivated is cultivated pH6～8,28～34 ℃ of culture temperature, incubation time 2～3 days; Inoculum size 10%～50% when the actinomycetes one-level is cultivated is cultivated pH6～8,27～32 ℃ of culture temperature, incubation time 5～7 days; Inoculum size 5%～20% when the milk-acid bacteria one-level is cultivated is cultivated pH6～8,28～34 ℃ of culture temperature, incubation time 2～5 days.
4. the preparation method of microbe soil restoration agent according to claim 2; It is characterized in that the photosynthetic bacterium secondary is cultivated and is intermittent type aerobic culture, inoculum size 20%～50% in the said step (2); Cultivate pH6～10; 28～36 ℃ of culture temperature, intensity of illumination 3000～4000 luxs, incubation time 7～10 days; The genus bacillus secondary is cultivated and is aerobic culture, and inoculum size 5%～15% is cultivated pH6～8,28～32 ℃ of culture temperature, incubation time 2～5 days; The actinomycetes secondary is cultivated and is aerobic culture, and inoculum size 20%～50% is cultivated pH6～8,27～32 ℃ of culture temperature, incubation time 5～7 days; The milk-acid bacteria secondary is cultivated to stuffiness and is cultivated, and inoculum size 5%～20% is cultivated pH6～8,28～34 ℃ of culture temperature, incubation time 2～5 days.
5. the preparation method of microbe soil restoration agent according to claim 2 is characterized in that, the photosynthetic bacterium fermentation culture is the intermittent type aerobic culture in the said step (3); Second class inoculum is inoculated in the 500L seeding tank, and inoculum size 20%～50% is cultivated pH7～8; 28～36 ℃ of culture temperature, intensity of illumination 3000～4000 luxs, incubation time 7～10 days; Be inoculated in 2 tons of fermentor tanks afterwards, inoculum size 20%～50% is cultivated pH7～8; 28～36 ℃ of culture temperature, intensity of illumination 3000～4000 luxs, incubation time 7～10 days; Fermentation of bacillus is cultivated to aerobic culture, second class inoculum is inoculated in the 200L seeding tank, inoculum size 5%～15%; Cultivate pH6～8,28～32 ℃ of culture temperature, incubation time 2～3 days; Be inoculated in 2 tons of fermentor tanks afterwards, inoculum size 20%～50% is cultivated pH7～8; 28～36 ℃ of culture temperature, incubation time 2～3 days; Actinomycete fermentation is cultivated to aerobic culture, second class inoculum is inoculated in the 500mL seeding tank, inoculum size 20%～50%; Cultivate pH6～8,27～32 ℃ of culture temperature, incubation time is inoculated in 2 tons of fermentor tanks after 5～7 days; Inoculum size 20%～50%; Cultivate pH7～8,28～36 ℃ of culture temperature, incubation time 7～10 days; Lactobacillus-fermented is cultivated to stuffiness and is cultivated, and second class inoculum is inoculated in the 200L seeding tank, inoculum size 5%～20%; Cultivate pH6～8,28～32 ℃ of culture temperature, incubation time 2～3 days; Be inoculated in 2 tons of fermentor tanks afterwards, inoculum size 5%～20% is cultivated pH7～8; 28～36 ℃ of culture temperature, incubation time 2～3 days.
6. the application of the described microbe soil restoration agent of claim 1 in rehabilitating soil.
7. the application of the described microbe soil restoration agent of claim 1 in tobacco, cotton, paddy rice, vegetables, melon and fruit plant husbandry.
8. the method for use of the described microbe soil restoration agent of claim 1 is characterized in that mixing use, usage quantity first with base manure, composite fertilizer: 2～3kg/ mu, later annual maintenance: 1～2kg/ mu.
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