KR20070090472A - The artificial mass-productions of oyster mushroom using roots of tissue-cultured mountain ginseng - Google Patents

Abstract

A method of artificially cultivating oyster mushroom(Pleurotus ostreatus) by addition of an extract, culture, meal or concentrate of tissue-cultured wild ginseng root during the cultivation of mushroom is provided. The method provides useful components such as saponin to mushroom and is environmentally friendly. During the artificial cultivation of mushroom, a tissue-cultured wild ginseng root extract, tissue-cultured wild ginseng root culture, tissue-cultured wild ginseng root meal or tissue-cultured wild ginseng root concentrate is added along with sawdust, cottonseed meal and beet pulp. The mushroom is Pleurotus ostreatus, Flammulina velutipes, Lentinus lepideus, Pleurotus eryngii and Lentinus edodes.

Description

 The artificial mass-productions of oyster mushroom using roots of tissue-cultured mountain ginseng}

The present invention relates to a method of artificial cultivation of wild ginseng root oyster mushroom.

Pleurotus eryngii is a higher fungus belonging to basidiomycetes and is the most consumed type of mushrooms produced in Korea. Oyster mushrooms are rich in fiber and have a good effect on diet, and contain protein, carbohydrates and fats. In particular, since it contains many polysaccharides such as beta glucan, it is effective for anti-cancer effects, and it is known to be a good food for preventing adult diseases due to its low cholesterol. As the consumption is steadily increasing, and mushrooms are fungi themselves, they cannot use pesticides such as antibacterial agents. It is attracting attention as an eco-friendly pesticide-free agricultural product.

The method of cultivating mushrooms by absorbing elements of a single substance known to be beneficial to our bodies, such as selenium or germanium, has been studied. However, the production of oyster mushrooms containing saponin from wild ginseng has not been successful. This could not be applied to relatively inexpensive mushrooms because of the rareness and high price of conventional ginseng, but recently, the development of wild ginseng cultured muscle culture technology, and the ginseng culture root, wild ginseng cultured gourd, wild ginseng extract, etc. It is expected to be able to increase the value of mushrooms by applying them to mushrooms, and to produce and supply high-functional mushrooms.

Wild ginseng cultivated roots used as a raw material for mushroom cultivation are grown aseptically on board, so there are no residual pesticides, no heavy metals, no harmful bacteria. In addition to saponins owned by ginseng such as Rb1, Rb2, Re, Rg1, Rg2, Rg3, Rh, etc., they contain special saponins that only wild ginseng such as Rf have, so it can regulate immune function, anticancer effect, anti-fatigue effect, and promote learning function. It has an excellent function and saponin content is higher than ordinary ginseng and has a different DNA structure than ginseng. Therefore, the research value of artificial cultivation method of high functional mushrooms containing saponin using these raw materials and by-products is very high.

In order to solve the limitations of the conventional functional mushroom production as described above, to provide an artificial cultivation method of wild ginseng culture root oyster mushroom.

Still another object of the present invention is to prepare and mix wild ginseng culture filtrate and wild ginseng culture roots in mushroom mycelium cultivation process, environment control during mushroom development and wild ginseng culture root extract in growth process to be effectively absorbed by mushrooms. Consists of steps.

The present invention relates to a method of artificial culture of wild ginseng mushroom using wild ginseng culture root extract and culture filtrate, wild ginseng culture root.

The wild ginseng culture root oyster mushroom of the present invention contains a special saponin which wild ginseng has, and has the function of wild ginseng with special effects, and therefore can be ingested together mushrooms and wild ginseng pharmacological effect Has the advantage of being superior.

In addition, another example of the present invention can use the remaining ginseng culture filtrate and the remaining gourd after extracting wild ginseng culture root in the culture medium in the culture process of wild ginseng root can be recycled by-products and tissue culture of wild ginseng culture root is mushroom culture and Likewise, since continuous production is possible, fresh raw materials can be secured continuously, which enables the production of wild ginseng root lodges steadily and stably along with wild ginseng tissue culture. Wild ginseng cultures are cultured for more than 2 months to produce and harvest wild ginseng culture roots. After harvesting, a significant amount of saponin is dissolved in the culture solution provided through wild ginseng tissue culture, and this culture filtrate containing saponin is cultivated when cultivated It will be used again as a culture medium. In addition, alcohol extract of wild ginseng tissue culture root is used for the product using the extract, and the remaining wild ginseng tissue culture root gourd as an extract by-product is recycled back to the mushroom medium.

In the present invention, after applying the ginseng culture root filtrate as a step of applying to the mushroom medium, the ginseng culture root gourd after the mushroom development and mushroom growth process, finally applied concentrated mountain ginseng cultured root extract can produce wild ginseng culture root oyster mushroom containing a large amount of ginseng cultured root components It is.

The temperature, roughness, carbon dioxide concentration, humidity, etc. for growing other oyster mushrooms are generally similar to methods known to those skilled in the art.

The following examples are intended to illustrate the present invention, the protection scope of the present invention is not limited to the following embodiments.

Example

Growing oyster mushrooms by adding wild ginseng root culture and wild ginseng root (bak) in medium production process

In the present invention, the mushroom spawn for cultivating wild ginseng root oyster mushroom was used Spring Cultivation No. 2, and the solid spawn prepared with sawdust was used. The wild ginseng cultivated root oyster mushroom medium was composed of sawdust 50%, beet pulp 30%, and cottonseed foil 20%. Each wild ginseng culture root and wild ginseng culture filtrate were cultivated using the table, and the results were compared and analyzed.

Each mushroom medium was weighed 600g in 850ml bottle of medium culture bottle, sterilized at 103 ℃ for 240 minutes, cooled to 18 ℃ and inoculated with 10g / bottle of inoculum prepared in advance for 30 days in 18-23 ℃ culture room. After incubation, the bacteria were scratched for 5 days, and groundwater was used to maintain 95% relative humidity for 3 days of growth.

[Table 1] Cultivation of Pleurotus eryngii Using Wild Ginseng Culture Root and Wild Ginseng Culture Root

Wild ginseng culture filtrate Wild ginseng culture moisture Saponin content Example 1      0.5g    300 ml    0.08mg / g Example 2     5ml    295ml    0.04 mg / g Example 3     5ml      0.5g    295ml    0.15ml / g Example 4    10 ml       1 g    290 ml    0.21ml / g

  As shown in the examples, saponins not contained in normal mushrooms were analyzed with 0.08 mg / g and 0.04 mg / g, respectively, when added to wild ginseng culture filtrate or wild ginseng cultured saponin. The content increased in proportion to the input concentration.

Example

Cultivation of Pleurotus eryngii Mushroom Using Wild Ginseng Root Extract during Growth

In the same way as in Example 4, up to the mushroom growth stage before growth, the wild ginseng root extract was mixed with groundwater and concentration instead of general groundwater to maintain the essential moisture for mushroom growth in the growth stage. Saponin content was analyzed when mushrooms were harvested at%

[Table 2]

Wild Ginseng Cultured Root Extract Concentration Processing time Saponin content Example 5     100mg / ml      6hr    0.58 mg / g Example 6     100mg / ml     12hr    0.92 mg / g Example 7     200 mg / ml     12hr    1.29mg / g

  As shown in Example 4 above, when the wild ginseng extract foil and wild ginseng culture filtrate were added only to the culture medium in the culture conditions, and only the groundwater was treated under the growing conditions, the saponin content was very low as 0.21 mg / g. In the same conditions as in Example 4, the saponin content was increased proportionally as the concentration of wild ginseng cultured root extract and the treatment time were longer under the growing conditions.

The present invention relates to an artificial cultivation method of wild ginseng culture root oyster mushroom, by cultivating oyster mushroom using wild ginseng cultured filtrate, wild ginseng cultured root extract and wild ginseng cultured root extract, which mushroom can absorb the saponin components that were not present in the existing mushroom components. In combination with the tissue culture technology of wild ginseng, it is possible to consume the excellent ingredients of eco-friendly mass-produced mushrooms and useful ingredients of wild ginseng at an affordable price, which has an advantage in preventing geriatric diseases and health.

Claims (1)

In the process of artificial cultivation of mushrooms, various kinds of wild ginseng roots, such as wild ginseng root extract, wild ginseng root culture, wild ginseng root, or wild ginseng root concentrate were applied to zelkova mushroom, enoki mushroom, pine mushroom, shiitake mushroom, shiitake mushroom, etc. How to grow high functional mushrooms containing saponins