CN110574638B

CN110574638B - A device that is used for field inoculation of delicious boletus

Info

Publication number: CN110574638B
Application number: CN201911037126.5A
Authority: CN
Inventors: 蔡静芸; 赵斌; 易筑刚; 展茂魁; 路纪芳; 孙亚茹
Original assignee: ZUNYI INSTITUTE OF FORESTRY
Current assignee: ZUNYI INSTITUTE OF FORESTRY
Priority date: 2019-10-29
Filing date: 2019-10-29
Publication date: 2021-10-19
Anticipated expiration: 2039-10-29
Also published as: CN110574638A

Abstract

The invention discloses a device for field inoculation of boletus edulis, which comprises: the belt body is sequentially provided with a plurality of cones along the length direction of the belt body, partial shallow layer roots of young saplings are dug out, the roots are dragged to the ground surface, the belt body is spirally wound on the roots along the length direction of the roots, the cone bodies are partially penetrated into the roots, hypha and nutrients of boletus edulis are filled in inner cavities of the cone bodies, a plurality of vent holes are formed in the bottom of the cone bodies, and degradation materials are mixed in the cone bodies. The cone with hyphae is punctured into the root of the young sapling by the winding belt body, so that the interference of mixed bacteria can be effectively reduced, and the operation is relatively simple.

Description

A device that is used for field inoculation of delicious boletus

Technical Field

The invention relates to the technical field of edible fungus cultivation, in particular to a device for field inoculation of boletus edulis.

Background

Boletus edulis belongs to one of Basidiomycotina, Hymenomycetes, Agaricales and Boletaceae, and is a worldwide distributed fungus with high economic value for both food and medicine. China has various provinces and high yield in southwest regions. The boletus edulis has fleshy and tender meat, aromatic flavor, delicious taste, rich nutrition and unique flavor. In addition to certain nutritive value, the product has higher medicinal value, and animal experiment and clinical research results show that the boletus edulis contains various alkaloids, and can treat lumbago, leg pain, numbness of hands and feet, discopathy, tetany of limbs, and abnormal leucorrhea of women; the polysaccharide and the basic protein can resist tumor and virus and regulate the immune function of human body. As a precious wild fungus, the boletus edulis is an important export mushroom in China, but because the boletus edulis is a mycorrhizal fungus which is symbiotic with higher plants and is difficult to grow on an artificial culture medium, the boletus edulis of commercial trade at present is all from wild resources collected in forests, and cannot meet the increasing demand.

In order to expand the yield of the semi-artificially cultured boletus edulis, the invention provides a device for field inoculation of the boletus edulis, which can reduce the interference of mixed bacteria and simplify the operation steps during inoculation.

Disclosure of Invention

The invention overcomes the defects of the prior art and provides a device for field inoculation of boletus edulis.

In order to achieve the purpose, the invention adopts the technical scheme that: a device for field inoculation of boletus edulis comprising: the belt body is sequentially provided with a plurality of cones along the length direction of the belt body, partial shallow roots of young saplings are dug out, the roots are dragged to the ground surface, the belt body is spirally wound on the roots along the length direction of the roots, the cone parts penetrate into the roots, hypha and nutrients of boletus edulis are filled in an inner cavity of each cone, a plurality of vent holes are formed in the bottom of each cone, and degradation materials are mixed in the cones.

In a preferred embodiment of the present invention, the degradable material is a starch-based degradable material.

In a preferred embodiment of the present invention, the cone is a plastic bottle which can be used as a container for low-pressure cultivation during the initial cultivation of hyphae.

In a preferred embodiment of the invention, each of said roots is pierced with 4-8 cones.

In a preferred embodiment of the present invention, after the root is wrapped with the tape and the cone is penetrated into the root, the surface of the root is covered with the soil slurry after the sterilization treatment.

In a preferred embodiment of the present invention, the edge of the band body is coated with a bactericide for preventing the mixture of foreign bacteria.

In a preferred embodiment of the invention, the tree species of the sapling is cyclobalanopsis glauca, and the age of the tree is 3-6 years.

In a preferred embodiment of the invention, the volume ratio of the materials in the cone to the air is 1 (20-40).

In a preferred embodiment of the present invention, the top of the cone is provided with an opening, and the opening is provided with a cutting edge.

In a preferred embodiment of the invention, the mass of hyphae of the strain in a single cone is 30-50 g.

The invention solves the defects in the background technology, and has the following beneficial effects:

(1) the invention is used for the inoculation of the field boletus edulis, the cone filled with hyphae is punctured into the root of the young sapling by the winding belt body, the interference of mixed bacteria can be reduced, and the operation is relatively simple.

(2) The material that the cone adopted can take place to degrade after a period, and the earlier stage of cone promptly can regard as the supporting body of protector and hypha, and the later stage can degrade and remove the relative encapsulated situation of hypha by oneself, simplifies the operating procedure, has improved efficiency.

(3) The cone is coated by the treated soil pulp, and a small amount of bactericide is coated on the edge of the belt body, so that a relatively sterile environment can be effectively provided for the growth of the bolete, and the growth of the bolete is facilitated.

Drawings

The invention is further explained below with reference to the figures and examples;

FIG. 1 is a schematic illustration of tree root placement in accordance with a preferred embodiment of the present invention;

FIG. 2 is a schematic view of the band and cone of the preferred embodiment of the present invention;

FIG. 3 is a schematic view of the opening of the cone of the preferred embodiment of the present invention;

fig. 4 is a schematic view of a preferred embodiment of the present invention showing the winding of the tape;

FIG. 5 is a schematic diagram of a preferred embodiment of the present invention after completion of inoculation;

in the figure: 1. a belt body; 2. a cone; 3. a blade; 4. soil slurry; 5. sapling; 6. a tree root; 7. and (7) a soil pit.

Detailed Description

The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.

As shown in fig. 1 to 5, a device for field inoculation of boletus edulis, comprising: the belt body 1 is sequentially provided with a plurality of cones 2 along the length direction of the belt body 1, partial shallow tree roots 6 of young saplings 5 are dug out, the tree roots 6 are dragged to the ground surface, the belt body 1 is spirally wound on the tree roots 6 along the length direction of the tree roots 6, the cone body part penetrates into the tree roots 6, hypha and nutrients of boletus edulis filled in an inner cavity of the cone body, a plurality of air holes are formed in the bottom of the cone body, and degradation materials are mixed in the cone body.

The inoculation steps of the invention are as follows: firstly, injecting cultured hyphae and a certain amount of materials required for growth into a cone 2, wherein the volume ratio of the materials in the cone 2 to air is about 1:30, the mass of the hyphae of a strain in a single cone 2 is about 40g, the top of the cone 2 is provided with an opening, a knife edge 3 is attached to the opening, the opening is conveniently penetrated and rotationally fixed on a tree root 6, and the material of the knife edge 3 is basically the same as that of the cone and can be automatically degraded. Selecting young seedlings 5 which are relatively healthy and have no obvious diseases in the green and old Onggu forest, wherein the ages of the seedlings 5 are 3-6 years, digging soil pits 7 at the roots of the seedlings 5, the thickness of each soil pit 7 is not more than 15cm, digging out relatively shallow healthy roots 6 of the seedlings 5 completely, placing a section of the roots 6 which is relatively uniform in thickness at the center of each soil pit 7, and inserting 4-8 cones 2 into the roots 6 of the section. (too much affects the uptake of nutrients by the bolete hyphae in each cone 2, and thus the yield.)

Cone 2 is fixed on the area body 1, and cone 2 can be the plastic bottle, is convenient for transport and can regard as the container that the hypha initial stage was cultivated the time low pressure and cultivate, can mix into partial starch base degradation material during the cone 2 preparation, will take the area body 1 winding to pierce tree root 6 in the one section that the thickness is even relatively, the top opening structure of cone 2. After the winding and fixing of the belt body 1 are finished, the processed soil slurry 4 is wrapped by the tree root 6 wound with the belt body 1 and is filled in the whole soil pit 7. Before winding, the belt body 1 can be coated with a small amount of bactericide at the edges of the two sides of the belt body 1 to prevent the mixed bacteria from the outside. Whether the mixture of material and hyphae stored in the cones 2 is in a liquid or solid state, all of the cones 2 need to penetrate the roots 6 from the area above the roots 6 in order to promote the rate of germination. And when the cone is buried in the soil slurry, the cone is inverted to ensure that a material hypha mixture in the cone is accumulated at the opening of the cone, the mixture is directly contacted with the incision of the tree root, and the hypha inoculation work is finished after the cone penetrates into the tree root. The time required for the lysis of the cone 2 in the whole area is within 3 months, while the ground-breaking time of the inoculated boletus edulis is generally different from 3-4 months to half a year.

In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. A device for field inoculation of boletus edulis comprising: the belt body is sequentially provided with a plurality of cones along the length direction of the belt body, hypha and nutrients of boletus edulis are filled in the inner cavities of the cones, the bottom of each cone is provided with a plurality of vent holes, and degradation materials are mixed in the cones; the edge of the belt body is coated with a bactericide for preventing the mixing of external bacteria; the top of the cone is provided with an opening, and a cutting edge is additionally arranged at the opening;

the inoculation method by adopting the device for field inoculation of boletus edulis comprises the following steps:

digging out part of shallow roots of young saplings, dragging the roots to the ground surface, spirally winding the belt body on the roots along the length direction of the roots, and penetrating the cone parts into the roots;

and after the tree root is wound by the belt body and the cone penetrates into the tree root, the surface of the tree root is coated by soil slurry subjected to sterilization treatment.

2. The device of claim 1, wherein the device is used for field inoculation of Boletus edulis, and the device comprises: the degradable material is starch-based degradable material.

3. The device of claim 1, wherein the device is used for field inoculation of Boletus edulis, and the device comprises: the cone is a plastic bottle which can be used as a container for low-pressure cultivation during the initial cultivation of hyphae.

4. The device of claim 1, wherein the device is used for field inoculation of Boletus edulis, and the device comprises: each of the roots is pierced with 4-8 cones.

5. The device of claim 1, wherein the device is used for field inoculation of Boletus edulis, and the device comprises: the tree species of the sapling is cyclobalanopsis glauca, and the tree age is 3-6 years.

6. The device of claim 1, wherein the device is used for field inoculation of Boletus edulis, and the device comprises: the volume ratio of the materials in the cone to the air is 1 (20-40).

7. The device of claim 1, wherein the device is used for field inoculation of Boletus edulis, and the device comprises: the mass of hyphae of the strain in a single cone is 30-50 g.