CN103858661A - Semi-artificial cultivation method for boletus edulis - Google Patents
Semi-artificial cultivation method for boletus edulis Download PDFInfo
- Publication number
- CN103858661A CN103858661A CN201210536275.8A CN201210536275A CN103858661A CN 103858661 A CN103858661 A CN 103858661A CN 201210536275 A CN201210536275 A CN 201210536275A CN 103858661 A CN103858661 A CN 103858661A
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- container
- inoculation
- root
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- boletus edulis
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- 241001489124 Boletus edulis Species 0.000 title claims abstract description 12
- 238000012364 cultivation method Methods 0.000 title abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 19
- 239000002689 soil Substances 0.000 claims abstract description 9
- 241000233866 Fungi Species 0.000 claims abstract description 7
- 241000168036 Populus alba Species 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims abstract description 4
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 claims abstract description 3
- 230000009286 beneficial effect Effects 0.000 claims abstract 2
- 230000001580 bacterial effect Effects 0.000 claims description 14
- 239000004033 plastic Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 238000002955 isolation Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 244000309464 bull Species 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 230000004720 fertilization Effects 0.000 claims description 2
- 230000035784 germination Effects 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 238000003973 irrigation Methods 0.000 claims description 2
- 230000002262 irrigation Effects 0.000 claims description 2
- 239000004571 lime Substances 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 238000003801 milling Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- -1 polypropylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 239000011435 rock Substances 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 239000003643 water by type Substances 0.000 claims description 2
- 238000009333 weeding Methods 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 2
- 238000012550 audit Methods 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 241001600007 Phlebopus portentosus Species 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000213788 Suillus aeruginascens Species 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- RYZCLUQMCYZBJQ-UHFFFAOYSA-H lead(2+);dicarbonate;dihydroxide Chemical compound [OH-].[OH-].[Pb+2].[Pb+2].[Pb+2].[O-]C([O-])=O.[O-]C([O-])=O RYZCLUQMCYZBJQ-UHFFFAOYSA-H 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a semi-artificial cultivation method for boletus edulis and other edible mycorrhiza fungi (EMF). Under trees of white poplar and other varieties of trees which can form mycorrhiza with boletus edulis, the strain of boletus edulis is inoculated to roots of young trees or audit trees by using a container inoculation method which can prevent soil microbial contamination, the sterile technique is fully utilized to locally create a relatively isolated environment with microbes as less as possible, so as to be beneficial to continuous growth of stain hyphae of the boletus edulis, and thus symbiosis of the boletus edulis with the roots of a symbiotic plant is successful; inoculation is performed under the tree one year or two years old in early spring, and then boletus edulis sporocarp grows and matures. According to the semi-artificial cultivation method for boletus edulis and other edible mycorrhiza fungi, by using the container inoculation method, the semi-artificial cultivation of the boletus edulis and other edible mycorrhiza fungi can be rapidly achieved and industrialization of the same can also be achieved rapidly; ecological agriculture can be rapidly developed, microbial diseases of the trees can be effectively reduced, the absorption area of the tree roots is enlarged, and the production of woods is improved. According to all semi-artificial cultivation methods for edible mycorrhiza fungi, the container inoculation method can be used for performing the inoculation to the root parts of symbiotic plants.
Description
Technical field
The present invention relates to cepe [Boletus edulis Bull.:Fr] and the semi-artificial cultivation method of other mycorhiza edible mushroom (EMF).
Background technology
At present, semi-artificial cultivation bolete is successfully detected in Phlebopus portentosus and the sticky bolete of brown ring, and the semi-artificial cultivation of cepe have not been reported; Mycorrhizal fungi inoculation method is divided into soil inoculation method, spore inoculating method, the disconnected inoculation method of root, VA Mycorrhizal Fungi transplanting inoculation method, solid or liquid mycelium inoculation and capsules strain inoculation method, has no the report of container inoculation method.
Summary of the invention
The present invention relates to cepe
[Boletus edulis Bull.:Fr]and other mycorhiza edible mushroom
(EMF) semi-artificial cultivation method, at silverwhite poplar or other can form under the tree of seeds of mycorhiza with cepe, on the root system of treelet or one-tenth tree in age, utilize the container inoculation method inoculation cepe bacterial classification that prevents soil living contaminants, can make full use of disinfection technology, the local the least possible environment of miscellaneous bacteria of creating a relative isolation, be conducive to the continued growth of cepe bacterial classification mycelia, so that cepe bacterial classification is in aulophyte root symbiosis success, early spring inoculation then or the cepe fruit body reaching maturity of growing under Second Year tree, utilize container inoculation method can realize fast cepe and the semi-artificial cultivation of other mycorhiza edible mushroom and realize fast industrialization, not only can greatly develop the ecological agriculture, can also effectively reduce the microorganism disease of trees, expand the absorption area of tree root, promote the output of timber.The semi-artificial cultivation method of mycorhiza edible mushroom (EMF) can utilize container inoculation method method to be inoculated into aulophyte root.
Embodiment
The semi-artificial cultivation of cepe, it is characterized in that carrying out according to the following steps: a, Isolation and Culture: field acquisition to cepe under sterile working, cut in time the tissue of a fritter between cap and stem, put into mother culture media and cultivate, within 10-15 days, grow mycelia, b, bacterial classification expands numerous: through wheat or the iblet of germination treatment, mix a little magnesium sulfate, dipotassium hydrogen phosphate and lime, after mixing, pack in vial or polypropylene strain bag, for expand numerous bacterial classification container adopt tampon or silica gel plug as stopper to meet the air requirements of mycelial growth, 120 DEG C of sterilizings 120 minutes, after cooling, can inoculate original seed mycelia, half-light was cultivated in 1 month, between culture period, rock several times, so that mycelia is evenly growth in whole container, approach cover with before for inoculating tree root, so that mycelia continued growth after inoculation, obtain the mycelia most with infecting potential, c, tree root inoculation: excavate white poplar root circle portion soil, expose the lateral root of root system, the processing of inoculation position cleaning and sterilizing, then 20-50 gram of bacterial classification put into container jaggy, container can be plastic bottles, paper bottle or plastic bag, paper bag, container is wanted slot milling, to meet the demand of mycelial growth to air, allow the container that cepe bacterial classification is housed near white poplar sterilization place tree root root, and allow tree root root embed in the breach of container, bacterial classification is fully contacted with plant root, then use closed material, can be plastic paper or paper, breach is closed, after having inoculated, fix, restore to the original state at inoculation position earthing, aforesaid operations, hand, tool in operation, container and closed material all will be through disinfecting, the method can effectively prevent the pollution of soil miscellaneous bacteria, d, cultivate fruiting: ridging, loosen the soil, weeding, can be after inoculation 15 days, sprinkler irrigation fertilization waters, inoculation in spring, general 3-4 month or Second Year can grow cepe fruit body in white poplar sylvan life, and the fruit body growing is the same with wild state.
Claims (2)
1. the semi-artificial cultivation of cepe [Boletus edulis Bull.:Fr], it is characterized in that carrying out according to the following steps: a, Isolation and Culture: field acquisition to cepe under sterile working, cut in time the tissue of a fritter between cap and stem, put into mother culture media and cultivate, within 10-15 days, grow mycelia, b, bacterial classification expands numerous: through wheat or the iblet of germination treatment, mix a little magnesium sulfate, dipotassium hydrogen phosphate and lime, after mixing, pack in vial or polypropylene strain bag, for expand numerous bacterial classification container adopt tampon or silica gel plug as stopper to meet the air requirements of mycelial growth, 120 DEG C of sterilizings 120 minutes, after cooling, can inoculate original seed mycelia, half-light was cultivated in 1 month, between culture period, rock several times, so that mycelia is evenly growth in whole container, approach cover with before for inoculating tree root, so that mycelia continued growth after inoculation, obtain the mycelia most with infecting potential, c, tree root inoculation: excavate white poplar root circle portion soil, expose the lateral root of root system, the processing of inoculation position cleaning and sterilizing, then 20-50 gram of bacterial classification put into container jaggy, container can be plastic bottles, paper bottle or plastic bag, paper bag, container is wanted slot milling, to meet the demand of mycelial growth to air, allow the container that cepe bacterial classification is housed near white poplar sterilization place tree root root, and allow tree root root embed in the breach of container, bacterial classification is fully contacted with plant root, then use closed material, can be plastic paper or paper, breach is closed, after having inoculated, fix, restore to the original state at inoculation position earthing, aforesaid operations, hand, operation tool, container and closed material all will be through disinfecting, the method can effectively prevent the pollution of soil miscellaneous bacteria, d, cultivate fruiting: ridging, loosen the soil, weeding, can be after inoculation 15 days, sprinkler irrigation fertilization waters, inoculation in spring, general 3-4 month or Second Year can grow cepe fruit body in white poplar sylvan life, and the fruit body growing is the same with wild state.
2. mycorhiza edible mushroom (EMF) can utilize container inoculation method to be inoculated into aulophyte root, the method makes full use of the technology of disinfecting in the woods out of doors, the local the least possible environment of miscellaneous bacteria of creating a relative isolation, be conducive to the continued growth of mycorhiza edible fungus species, be beneficial to bacterial classification in aulophyte root symbiosis success, realize the object of semi-artificial cultivation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210536275.8A CN103858661A (en) | 2012-12-07 | 2012-12-07 | Semi-artificial cultivation method for boletus edulis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210536275.8A CN103858661A (en) | 2012-12-07 | 2012-12-07 | Semi-artificial cultivation method for boletus edulis |
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| Publication Number | Publication Date |
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| CN103858661A true CN103858661A (en) | 2014-06-18 |
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| CN201210536275.8A Pending CN103858661A (en) | 2012-12-07 | 2012-12-07 | Semi-artificial cultivation method for boletus edulis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875185A (en) * | 2014-11-24 | 2016-08-24 | 黑龙江省林副特产研究所 | Artificial symbiotic cultivation method for fungi |
| CN106134769A (en) * | 2016-07-20 | 2016-11-23 | 贵州师范大学 | A kind of manufacture method of Boletus edulis Bull ex Franch original seed |
| JP2017038541A (en) * | 2015-08-18 | 2017-02-23 | ホクト株式会社 | Cultivation method of boletus |
| CN110574638A (en) * | 2019-10-29 | 2019-12-17 | 遵义市林业科学研究所 | a device that is used for field inoculation of delicious boletus |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02142423A (en) * | 1988-11-22 | 1990-05-31 | Tomoaki Otsuka | Preparation of mycorrhiza-formable fungus |
| JPH05316876A (en) * | 1992-05-21 | 1993-12-03 | New Oji Paper Co Ltd | Method of forming matsutake white |
| JPH05328841A (en) * | 1992-05-29 | 1993-12-14 | Pentel Kk | Mycorrhizal mycorrhizal method |
| TW283622B (en) * | 1993-09-28 | 1996-08-21 | House Food Industrial Co | |
| CN101669430A (en) * | 2009-09-24 | 2010-03-17 | 云南省热带作物科学研究所 | Semi-artificial culture method of phlebopus portentosus |
| CN102715013A (en) * | 2012-06-19 | 2012-10-10 | 云南大学 | Culture method for inhibiting browning of bolete mycelium |
-
2012
- 2012-12-07 CN CN201210536275.8A patent/CN103858661A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02142423A (en) * | 1988-11-22 | 1990-05-31 | Tomoaki Otsuka | Preparation of mycorrhiza-formable fungus |
| JPH05316876A (en) * | 1992-05-21 | 1993-12-03 | New Oji Paper Co Ltd | Method of forming matsutake white |
| JPH05328841A (en) * | 1992-05-29 | 1993-12-14 | Pentel Kk | Mycorrhizal mycorrhizal method |
| TW283622B (en) * | 1993-09-28 | 1996-08-21 | House Food Industrial Co | |
| CN101669430A (en) * | 2009-09-24 | 2010-03-17 | 云南省热带作物科学研究所 | Semi-artificial culture method of phlebopus portentosus |
| CN102715013A (en) * | 2012-06-19 | 2012-10-10 | 云南大学 | Culture method for inhibiting browning of bolete mycelium |
Non-Patent Citations (3)
| Title |
|---|
| 付绍春 等: "美味牛肝菌生态特性与栽培技术研究进展", 《湖南林业科技》 * |
| 弓明钦 等: "《菌根型食用菌及其半人工栽培》", 31 August 2007 * |
| 纪开萍 等: "暗褐网柄牛肝菌半人工模拟栽培及"宿主树"根系上菌丝生长的持久性", 《微生物学通报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875185A (en) * | 2014-11-24 | 2016-08-24 | 黑龙江省林副特产研究所 | Artificial symbiotic cultivation method for fungi |
| JP2017038541A (en) * | 2015-08-18 | 2017-02-23 | ホクト株式会社 | Cultivation method of boletus |
| CN106134769A (en) * | 2016-07-20 | 2016-11-23 | 贵州师范大学 | A kind of manufacture method of Boletus edulis Bull ex Franch original seed |
| CN110574638A (en) * | 2019-10-29 | 2019-12-17 | 遵义市林业科学研究所 | a device that is used for field inoculation of delicious boletus |
| CN110574638B (en) * | 2019-10-29 | 2021-10-19 | 遵义市林业科学研究所 | A device that is used for field inoculation of delicious boletus |
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Application publication date: 20140618 |