CN104488549B - The high temperature of HUAZIGU goes out mushroom cultivation method - Google Patents
The high temperature of HUAZIGU goes out mushroom cultivation method Download PDFInfo
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- CN104488549B CN104488549B CN201410775560.4A CN201410775560A CN104488549B CN 104488549 B CN104488549 B CN 104488549B CN 201410775560 A CN201410775560 A CN 201410775560A CN 104488549 B CN104488549 B CN 104488549B
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- culture medium
- huazigu
- temperature
- cultivating bag
- high temperature
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 23
- 238000012364 cultivation method Methods 0.000 title claims abstract description 11
- 239000001963 growth medium Substances 0.000 claims abstract description 24
- 230000001954 sterilising effect Effects 0.000 claims abstract description 16
- 230000035784 germination Effects 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 239000002023 wood Substances 0.000 claims abstract description 8
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 7
- 235000013312 flour Nutrition 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 7
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 6
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims abstract description 6
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 6
- 238000011177 media preparation Methods 0.000 claims abstract description 4
- 238000012258 culturing Methods 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 229920000742 Cotton Polymers 0.000 claims description 4
- 241000233866 Fungi Species 0.000 claims description 4
- 238000005057 refrigeration Methods 0.000 claims description 4
- 235000007685 Pleurotus columbinus Nutrition 0.000 claims description 3
- 240000001462 Pleurotus ostreatus Species 0.000 claims description 3
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims description 3
- 239000011121 hardwood Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 244000168667 Pholiota nameko Species 0.000 description 2
- 235000014528 Pholiota nameko Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000002686 mushroom body Anatomy 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 241000722337 Pholiota Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 240000006794 Volvariella volvacea Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 235000019628 coolness Nutrition 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The high temperature that the invention discloses a kind of HUAZIGU goes out mushroom cultivation method, comprises the steps: culture medium preparation, culture medium pack, normal-pressure sterilization, cools down, inoculates, training orientation, opens bag, accelerating germination management, growth management, gathers.Wherein, the formula of culture medium includes the raw material of following mass percent: wood flour 65～75%, wheat bran 15～20%, calcium superphosphate 0.5～2%, Calx 0.5～2%, Gypsum Fibrosum 0.5～2%.The HUAZIGU not only yield that the inventive method produces is high, and complete appearance is attractive in appearance, and can realize the high temperature season fresh mushroom of supply, it is possible to meet the needs in market；Production cost is low, is beneficial to realize objective economic benefit, carries out popularization and application.
Description
Technical field
The present invention relates to fungus growing technique field, the high temperature fruiting particularly relating to a kind of HUAZIGU is planted
Culture method.
Background technology
HUAZIGU has another name called Pholiota nameko, Pholiota nameko, and Japan cries and receives U.S. mushroom, formal name used at school pholiotanamekio.
On botany classification belong to Eumycota, Basidiomycotina, Basidiomycetes, Agaricales, cortina Cordycepps,
Pholiota.Belong to rare kind, originate in Japan, from the seventies middle period, start from south, Liaoning Province
Area, existing main producing region is the ground such as northern Hebei, Liaoning, Heilungkiang.
HUAZIGU is a kind of low form mushrooms, and the cultivation of HUAZIGU is currently with wood flour as major ingredient, then joins
With Testa Tritici, Calx and some additives as adjuvant, along with culturing raw material reduces increasingly, its cultivation yield
And cultivation is regional, environmental condition is all restricted, it is impossible to meet the demand in market.
Summary of the invention
The technical problem to be solved is to provide a kind of cultivation low cost, yield height, is suitable to work
The high temperature of the HUAZIGU that factory's metaplasia is produced goes out mushroom cultivation method.
For solving above-mentioned technical problem, the technical scheme is that
The high temperature of HUAZIGU goes out mushroom cultivation method, comprises the steps:
(1) culture medium preparation
Culture medium prescription includes the raw material of following mass percent: wood flour 65～75%, wheat bran 15～20%,
Calcium superphosphate 0.5～2%, Calx 0.5～2%, Gypsum Fibrosum 0.5～2%；Wherein, wood flour preferably employs wealthy
Leaf tree hardwood is considered to be worth doing, and wheat bran is as nutrient source.
The mixing of various raw materials being mixed thoroughly by culture medium prescription, moisture content in medium is 63～65%, pH value
7～7.5；
(2) culture medium pack
Culture medium is dispensed in pocket, the pocket of charged is put and forms cultivating bag without cotton neck ring；
(3) normal-pressure sterilization
Cultivating bag is carried out normal-pressure sterilization, and sterilising temp is maintained at more than 98 DEG C, continues sterilizing 8h；
(4) cooling
Cultivating bag after sterilizing is cooled to less than 22 DEG C in advance；
(5) inoculation
Sterile working；The inoculum concentration of strain is inoculation tree fungus one in every cultivating bag；
(6) training orientation
Culturing room is first cleaned, sterilizing；Postvaccinal cultivating bag is put in the culturing room of dark,
Training orientation is divided into early stage to cultivate, mid-term cultivates, the later stage cultivates three phases, the humidity of three phases
Control 60～70%, wherein:
Early stage is cultivated: cultivates indoor temperature and controls at 15～18 DEG C, training orientation 30～40d, cultivation
In bag, temperature controls below 22 DEG C；
Mid-term cultivates: cultivates indoor temperature and controls at 20～22 DEG C, and training orientation 20～30d is carried out
Scattered light is irradiated, and annesl, in cultivating bag, temperature controls below 25 DEG C；
Later stage cultivates: cultivates indoor temperature and controls, at 18～23 DEG C, to proceed the cultivation of 30～40d,
Control to cultivate indoor temperature and form the temperature difference, lay foundation for fruiting, culturing room's complete darkness, cultivating bag
Temperature controls at 20 ± 0.5 DEG C；
(7) bag is opened
Cultured cultivating bag is transported to mushroom room, takes off neck ring, cut bottom the pocket of bacterium rod growing
Fall, cut water supplement port in the bottom of culture medium, return moisturizing in time after bacterium；
(8) accelerating germination management
Accelerating germination condition: temperature controls at 12～18 DEG C, humidity >=90%, CO2Concentration 1800ppm with
Under；Illuminance is 300～800Lux；Germination time is 10～15d；
(9) growth management
Temperature controls at 12～18 DEG C, humidity >=90%, CO2Concentration 1300～1600ppm, fruiting
Peak period adds forced ventilation, and oxygen, temperature, humidity three are coordinated；
(10) gather
When HUAZIGU mushroom umbrella present Pleurotus ostreatus top, light yellow time, gather in time.
As preferably, in step (1), culture medium prescription includes the raw material of following mass percent: wood
Bits 75%, wheat bran 22%, calcium superphosphate 1%, Calx 1%, Gypsum Fibrosum 1%.
As preferably, in step (6), when the later stage cultivates, control temperature back and forth by refrigeration unit, control
The temperature difference in culturing room processed.
As preferably, in step (7), cut " well " font water supplement port in the bottom of culture medium.
Owing to have employed technique scheme, the HUAZIGU not only yield that the inventive method produces is high, and
And complete appearance is attractive in appearance, and the high temperature season fresh mushroom of supply can be realized, it is possible to meet the needs in market；Raw
Produce low cost, be beneficial to realize objective economic benefit, carry out popularization and application.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.
The high temperature of HUAZIGU goes out mushroom cultivation method, comprises the steps:
(1) culture medium preparation
Culture medium prescription includes the raw material of following mass percent: broad leaf tree hardwood bits 75%, wheat bran 22%,
Calcium superphosphate 1%, Calx 1%, Gypsum Fibrosum 1%.The pocket dress 0.5kg siccative of each 17cm × 38cm；
The mixing of various raw materials being mixed thoroughly by culture medium prescription, moisture content in medium is 63～65%, pH value
7～7.5；
(2) culture medium pack
Culture medium punching press sack filling machine is dispensed in plastic bag, the pocket of charged is put without cotton neck
Circle forms cultivating bag；
(3) normal-pressure sterilization
Cultivating bag being carried out normal-pressure sterilization, cultivating bag is placed on plastic crate, entrucking is shifted onto in autoclave,
Going out steam bottom autoclave, temperature reaches 99 DEG C, timing and continuous 8h；
(4) cooling
Shift the cultivating bag after sterilizing onto fore-cooling room, lower the temperature by refrigeration unit, be cooled to less than 22 DEG C in advance；
(5) inoculation
Normal sterile operates；The inoculum concentration of strain is tree fungus one in every cultivating bag；
(6) training orientation
Culturing room is first cleaned, and sterilizes with antibacterial；Postvaccinal cultivating bag is put into the culturing room of dark
In, training orientation is divided into early stage to cultivate, mid-term cultivates, the later stage cultivates three phases, three phases
Humid control is 60～70%, wherein:
Early stage is cultivated: cultivates indoor temperature and controls at 15～18 DEG C, training orientation 30～40d, cultivation
In bag, temperature controls below 22 DEG C；
Mid-term cultivates: cultivates indoor temperature and controls at 20～22 DEG C, and training orientation 20～30d is carried out
Scattered light is irradiated, and annesl, in cultivating bag, temperature controls below 25 DEG C；
Later stage cultivates: cultivates indoor temperature and controls, at 18～23 DEG C, to proceed the cultivation of 30～40d,
Control temperature back and forth by refrigeration unit, manual control is cultivated indoor temperature and is formed the temperature difference, beats base for fruiting
Plinth, culturing room's complete darkness, the temperature of cultivating bag controls at 20 ± 0.5 DEG C；
(7) bag is opened
Cultured cultivating bag is transported to freezer mushroom room, takes off without cotton neck ring, growing moulding of bacterium rod
Pocket and bottom plastic slice off, and cut one " well " word mouth in the bottom of culture medium, return moisturizing in time after bacterium；
(8) accelerating germination management
Accelerating germination condition: temperature controls at 12～18 DEG C, humidity >=90%, CO2Concentration 1800ppm with
Under；Illuminance is 300～800Lux；Germination time is 10～15d；
(9) growth management
Temperature controls at 12～18 DEG C, humidity >=90%, CO2Concentration 1300～1600ppm, fruiting
Peak period adds forced ventilation, and oxygen, temperature, humidity three are coordinated；
(10) gather
When HUAZIGU mushroom umbrella present Pleurotus ostreatus top, light yellow time, gather in time.
(11) postharvest handling
HUAZIGU puts into 2～5 DEG C of freezer pre-coolings after gathering, during pre-cooling, every basket is staggered and stacks；For
Domestic market sales need pre-cooling 4h, need more than pre-cooling 8h for foreign markets；Cut head to exist
Carry out under the conditions of 18 DEG C, prune unclean epidermis and arrangement mushroom shape；Classification is carried out during cutting mushroom,
Mushroom bodies at different levels require mushroom lid rounding, and mushroom body is uniform, fresh, pure white；Use polyethylene knuckle plastic bag
Subpackage, evacuation preserves in tightening bag mouth and being moved into 0 DEG C of freezer.
The foregoing is only the schematic detailed description of the invention of the present invention, be not limited to the present invention's
Scope.Any those skilled in the art, institute on the premise of without departing from the design of the present invention and principle
The equivalent variations made and amendment, all should belong to the scope of protection of the invention.
Claims (5)
1. the high temperature of HUAZIGU goes out mushroom cultivation method, it is characterised in that comprise the steps:
(1) culture medium preparation
Culture medium prescription includes the raw material of following mass percent: wood flour 65～75%, wheat bran 15～20%,
Calcium superphosphate 0.5～2%, Calx 0.5～2%, Gypsum Fibrosum 0.5～2%；
By culture medium prescription by various raw materials mixing mix thoroughly, moisture content in medium is 63～65%, pH value 7～
7.5；
(2) culture medium pack
Culture medium is dispensed in pocket, the pocket of charged is put neck ring and forms cultivating bag；
(3) normal-pressure sterilization
Cultivating bag is carried out normal-pressure sterilization, and sterilising temp is maintained at more than 98 DEG C, continues sterilizing 8h；
(4) cooling
Cultivating bag after sterilizing is cooled to less than 22 DEG C in advance；
(5) inoculation
Sterile working；The inoculum concentration of strain is inoculation tree fungus one in every cultivating bag；
(6) training orientation
Culturing room is first cleaned, sterilizing；Postvaccinal cultivating bag is put in the culturing room of dark, training
Foster management is divided into early stage to cultivate, mid-term cultivates, the later stage cultivates three phases, the humid control of three phases
60～70%, wherein:
Early stage is cultivated: cultivates indoor temperature and controls at 15～18 DEG C, training orientation 30～40d, cultivating bag
Interior temperature controls below 22 DEG C；
Mid-term cultivates: cultivates indoor temperature and controls at 20～22 DEG C, and training orientation 20～30d dissipates
Penetrating light to irradiate, annesl, in cultivating bag, temperature controls below 25 DEG C；
Later stage cultivates: cultivates indoor temperature and controls, at 18～23 DEG C, to proceed the cultivation of 30～40d,
Control temperature by refrigeration unit back and forth, control to cultivate indoor temperature and form the temperature difference, lay foundation for fruiting, training
Supporting room complete darkness, the temperature in cultivating bag controls at 20 ± 0.5 DEG C；
(7) bag is opened
Cultured cultivating bag is transported to mushroom room, takes off neck ring, slice off bottom the pocket of bacterium rod growing,
Cut " well " font water supplement port in the bottom of culture medium, return moisturizing in time after bacterium；
(8) accelerating germination management
Accelerating germination condition: temperature controls at 12～18 DEG C, humidity >=90%, CO2Concentration 1800ppm with
Under；Illuminance is 300～800Lux；Germination time is 10～15d；
(9) growth management
Temperature controls at 12～18 DEG C, humidity >=90%, CO2Concentration is 1300～1600ppm, and fruiting is high
The peak phase adds forced ventilation；
(10) gather.
2. the high temperature of HUAZIGU as claimed in claim 1 goes out mushroom cultivation method, it is characterised in that: step
Suddenly, in (1), culture medium prescription includes the raw material of following mass percent: wood flour 75%, wheat bran 22%,
Calcium superphosphate 1%, Calx 1%, Gypsum Fibrosum 1%.
3. the high temperature of HUAZIGU as claimed in claim 1 goes out mushroom cultivation method, it is characterised in that: institute
State wood flour and use broad leaf tree hardwood bits.
4. the high temperature of HUAZIGU as claimed in claim 1 goes out mushroom cultivation method, it is characterised in that: step
Suddenly, in (2), described neck ring uses without cotton neck ring.
5. the high temperature of HUAZIGU as claimed in claim 1 goes out mushroom cultivation method, it is characterised in that: step
Suddenly in (10), when HUAZIGU mushroom umbrella present Pleurotus ostreatus top, light yellow time, gather in time.
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| CN201410775560.4A CN104488549B (en) | 2014-12-15 | 2014-12-15 | The high temperature of HUAZIGU goes out mushroom cultivation method |
Applications Claiming Priority (1)
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| CN201410775560.4A CN104488549B (en) | 2014-12-15 | 2014-12-15 | The high temperature of HUAZIGU goes out mushroom cultivation method |
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| CN104488549A CN104488549A (en) | 2015-04-08 |
| CN104488549B true CN104488549B (en) | 2016-08-17 |
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| CN201410775560.4A Expired - Fee Related CN104488549B (en) | 2014-12-15 | 2014-12-15 | The high temperature of HUAZIGU goes out mushroom cultivation method |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866191A (en) * | 2015-12-14 | 2017-06-20 | 灵武市森保科技开发有限公司 | A kind of edible fungus stick that branch is pruned containing jujube tree and preparation method thereof |
| CN106212049A (en) * | 2016-08-24 | 2016-12-14 | 莒县鑫垚食用菌研究所 | A kind of Pholiota nameko inoculation method |
| CN108834755A (en) * | 2018-08-13 | 2018-11-20 | 重庆市映山红食用菌有限公司 | The cultural method of ripe pholiota nameko in a kind of |
| CN111919664A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Pholiota nameko culture medium and pholiota nameko culture method |
| CN112119845A (en) * | 2020-09-28 | 2020-12-25 | 马鞍山源之美农业科技有限公司 | Seedling raising process for vegetable seeds |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1363207A (en) * | 2001-10-26 | 2002-08-14 | 赵忠阳 | Prescription for culture of mushroom by fermentation with raw materials and culturing method thereof |
| JP2004147545A (en) * | 2002-10-30 | 2004-05-27 | Chikuma Kasei:Kk | Method for cultivating oudemansiella venosolamellata |
| JP4429085B2 (en) * | 2004-06-08 | 2010-03-10 | 有限会社コンペックス | Method for producing sterilized fungus bed composition for straw cultivation and straw cultivation method |
| CN101941866A (en) * | 2010-08-10 | 2011-01-12 | 葛树军 | Pholiota nameko(T.Tto)S.Ito et Imai) facility culture substrate prescription and production process thereof |
| CN101946633A (en) * | 2010-08-25 | 2011-01-19 | 庄河市农业技术推广中心 | Method for culturing pholiota nameko without pergola |
| CN102144496B (en) * | 2011-03-29 | 2012-12-05 | 福建绿宝食品集团有限公司 | Method for cultivating nameko mushrooms |
| CN102613006B (en) * | 2012-04-20 | 2014-06-25 | 沈阳蕈丰食用菌科技有限公司 | Pholiota nameko culture method |
| CN102726214A (en) * | 2012-07-06 | 2012-10-17 | 福建农林大学 | Method for cultivating organic Pholiota namek with weeds |
| CN103420723A (en) * | 2013-07-26 | 2013-12-04 | 邬金飞 | Pholiota nameko cultivation material compatibility and method for preparing cultivation material |
| CN103881931B (en) * | 2014-04-04 | 2016-01-20 | 王会森 | A kind of HUAZIGU and cultivating method thereof |
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