CN101946635A - Method for cultivating needle mushroom by utilziing distilled grains - Google Patents
Method for cultivating needle mushroom by utilziing distilled grains Download PDFInfo
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Abstract
The invention discloses a method for cultivating needle mushroom by utilizing distilled grains, comprising the following steps: preparing a culture medium, producing mushroom bags, sterilizing in a high-pressure sterilization cabinet or a normal-pressure sterilization oven, inoculating strains by an aseptic technique, culturing the mushroom, promoting buds, controlling the growth and development environment of fruiting bodies and the like. The method has the beneficial effects that the distilled grains, cotton seed hulls and bran are used for preparing the culture medium, thus having low cost; and the obtained needle mushroom has the advantages of balanced nutrition, orderly growth and excellent quality.
Description
Technical field
The present invention relates to a kind of cultivation method of Asparagus, particularly relate to a kind of vinasse cultivating flammulina velutipes method.
Background technology
Asparagus is a kind of rich nutrition edible fungus, and wherein zinc content has the effect that promotes children ' s intelligence development and brain tonic than higher, is described as " intelligence development mushroom " and " increasing the intelligence mushroom " in many countries such as Japan.Often edible Asparagus not only can prevent and treat diseases such as hepatopathy and stomach, enteron aisle ulcer, and can resisting fatigue, anti-inflammation, elimination heavy metal salt material, antineoplastic action.
Asparagus grows needs carbon source, nitrogenous source, mineral matter, nutriments such as vitamin, prior art is often selected cotton seed hulls for use, corncob, wheat bran, wood chip, raw material such as lime are mixed with the nutrient source of solid culture medium as the Asparagus growth, a kind of medium of Asparagus is disclosed as Chinese invention patent ublic specification of application CNl879463, the prescription and the weight proportion of its raw material are as follows: cotton seed hulls 30～40%, art bits 30～40%, wheat bran 25～30%, calcium carbonate 1%, because Asparagus decomposes lignin, a little less than the cellulose ability, this is the nutritional need that the prescription of main nutrient source is unfavorable for the Asparagus growth with wood chip, but also exist nutrition unbalanced, it is irregular to grow, and causes prolong degradation drawback under the quality picking time.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of vinasse cultivating flammulina velutipes method is provided, this method adopts vinasse, cotton seed hulls and wheat bran preparation medium, and the Asparagus that plants is balanced in nutrition, and growth is neat, and is best in quality.
Goal of the invention of the present invention is achieved through the following technical solutions: a kind of vinasse cultivating flammulina velutipes method, and it may further comprise the steps:
(1) medium preparation: culture medium prescription is vinasse 30～50%, cotton seed hulls 40～60% and wheat bran 5～10%, the culture medium preparation method is: add 3～4% lime in vinasse, regulate pH value to 6～7 of vinasse, mix by proportioning with cotton seed hulls, wheat bran then and mix thoroughly, add entry again, mix, make moisture content in medium reach 60～65%;
Vinasse are the mixture of wheat and rice hulls, or the mixture of iblet and rice hulls is the vinasse that raw material forms.
(2) the bacterium bag is produced: with medium pack into polypropylene plastics pocket or polyethylene plastic bag, the plastic sack specification is folding footpath 17～22cm, length 30～33cm, inserting diameter at the pocket middle part then is 2cm, length is 13～14cm sticking plaster, set up the neck ring that diameter is 5cm at sack, seal with plastic film;
(3) sterilization: with pocket pack into the sterilization frame in or the layer frame in, 6 in every frame or every layer charging bag, in autoclave cabinet or normal-pressure sterilization kitchen range, sterilize, when in autoclave cabinet, sterilizing, at pressure is sterilization treatment 3～4 hours under 0.1～0.2 MPa pressure, when in the normal-pressure sterilization kitchen range, sterilizing, be 98～100 ℃ in temperature and handled 18～20 hours down;
(4) inoculation: when pocket was cooled to below 28 ℃, sterile working inserted bacterial classification, during inoculation sticking plaster in the pocket was taken out, and bacterial classification is put into bag and is pressed into the pocket middle part, covered the pocket surface simultaneously, and sack seals with aseptic paper;
(5) cultivate a bacterium: cultivating indoor cultivation, indoor temperature is controlled at 19 ℃～25 ℃, and mycelial growth after the purseful of perhaps growing, begins to enter management of producing mushroom apart from the low 2～3cm of bag;
(6) urge flower bud: change neck ring on the bacterium bag sack into into 7cm neck ring, dig out sack endosexine bacterial classification then, thickness is 1～2cm, add water then in bag, amount of water is 30～50mL, uprightly discharge 1 day after, with bacterium bag handstand discharge water, make sack not have ponding, uprightly be emitted on the bedstead then, perhaps accumbency is on ground or bedstead, covered with plastic film is preserved moisture, and temperature is controlled at 10～15 ℃, and intensity of illumination is controlled at 5～10 luxs, after fruit body forms, take off and get plastic film;
(7) sporophore growth developing environment control: the bacterium bag that will form original hase or grow a large amount of young mushrooms uprightly is emitted on the bedstead, and temperature is controlled at 5～10 ℃, and intensity of illumination is controlled at 3～5 luxs, CO
2Concentration is controlled at 7000～9000mg/kg, when fruit body grows into 4～6cm when high, put plastic sack on sack, the plastic sack specification is 18～22cm for the folding footpath, and length is 33～45cm, one end of plastic sack is fixed on the sack, open plastic sack and make it to be tubular, pricking sack, and stay a hole with the elastic ring, the aperture is 1～2cm, gathers when fruit body length reaches 16～20cm length.
The invention has the beneficial effects as follows: adopt vinasse, cotton seed hulls and wheat bran preparation medium, cost is low, and the Asparagus that plants is balanced in nutrition, and growth is neat, and is best in quality.
Embodiment
Further describe technical scheme of the present invention below in conjunction with embodiment, but protection scope of the present invention is not limited to described embodiment.
Embodiment 1:A kind of vinasse cultivating flammulina velutipes method, it may further comprise the steps:
(1) medium preparation: culture medium prescription is vinasse 30%, cotton seed hulls 60% and wheat bran 10%, the culture medium preparation method is: the lime of adding 3% in vinasse, regulate the pH value to 6 of vinasse, mix by formula rate with cotton seed hulls, wheat bran then and mix thoroughly, add entry again, mix, make moisture content in medium reach 60%.
Wherein, described vinasse are that the mixture of wheat and rice hulls is the vinasse that raw material forms.
(2) the bacterium bag is produced: with the medium polypropylene plastics pocket of packing into, the plastic sack specification is folding footpath 17cm, length 30cm, and inserting diameter at the pocket middle part then is 2cm, length is the 13cm sticking plaster, sets up the neck ring that diameter is 5cm at sack, seals with plastic film.
(3) sterilization: pocket is packed in the sterilization frame, and 6 in every frame charging bag is sterilized in autoclave cabinet, is sterilization treatment 4 hours under the 0.1 MPa pressure at pressure.
(4) inoculation: when pocket was cooled to 25 ℃, sterile working inserted bacterial classification, during inoculation sticking plaster in the pocket was taken out, and bacterial classification is put into bag and is pressed into the pocket middle part, covered the pocket surface simultaneously, and sack seals with aseptic paper.
(5) cultivate a bacterium: cultivating indoor cultivation, indoor temperature is controlled at 19 ℃, and mycelial growth begins to enter management of producing mushroom apart from low 2 cm of bag.
(6) urge flower bud: change neck ring on the bacterium bag sack into into 7cm neck ring, dig out sack endosexine bacterial classification then, thickness is 1cm, add water then in bag, amount of water is 30mL, uprightly discharge 1 day after, with bacterium bag handstand discharge water, make sack not have ponding, uprightly be emitted on the bedstead then, perhaps accumbency is on ground or bedstead, covered with plastic film is preserved moisture, and temperature is controlled at 10 ℃, and intensity of illumination is controlled at 10 luxs, after fruit body forms, take off and get plastic film.
(7) sporophore growth developing environment control: the bacterium bag that will form original hase or grow a large amount of young mushrooms uprightly is emitted on the bedstead, and temperature is controlled at 8 ℃, and intensity of illumination is controlled at 5 luxs, CO
2Concentration is controlled at 7000mg/kg, when fruit body grows into 4cm when high, put plastic sack on sack, the plastic sack specification is 22cm for the folding footpath, and length is 45cm, one end of plastic sack is fixed on the sack, open plastic sack and make it to be tubular, pricking sack, and stay a hole with the elastic ring, the aperture is 2cm, gathers when fruit body length reaches 20cm length.
Embodiment 2:A kind of vinasse cultivating flammulina velutipes method, it may further comprise the steps:
(1) medium preparation: culture medium prescription is vinasse 40%, cotton seed hulls 50% and wheat bran 10%, the culture medium preparation method is: the lime of adding 4% in vinasse, regulate the pH value to 7 of vinasse, mix by proportioning with cotton seed hulls, wheat bran then and mix thoroughly, add entry again, mix, make moisture content in medium reach 65%;
Vinasse are that the mixture of iblet and rice hulls is the vinasse that raw material forms.
(2) the bacterium bag is produced: with the medium polyethylene plastic bag of packing into, the plastic sack specification is folding footpath 22cm, length 33cm, and inserting diameter at the pocket middle part then is 2cm, length is the 14cm sticking plaster, sets up the neck ring that diameter is 5cm at sack, seals with plastic film;
(3) sterilization: pocket is packed in layer frame, and 6 in every layer of charging bag sterilized in the normal-pressure sterilization kitchen range, is 98 ℃ in temperature and handles 20 hours down;
(4) inoculation: when pocket was cooled to 20 ℃, sterile working inserted bacterial classification, during inoculation sticking plaster in the pocket was taken out, and bacterial classification is put into bag and is pressed into the pocket middle part, covered the pocket surface simultaneously, and sack seals with aseptic paper;
(5) cultivate a bacterium: cultivating indoor cultivation, indoor temperature is controlled at 20 ℃, and mycelial growth begins to enter management of producing mushroom apart from the low 3cm of bag;
(6) urge flower bud: change neck ring on the bacterium bag sack into into 7cm neck ring, dig out sack endosexine bacterial classification then, thickness is 2cm, add water then in bag, amount of water is 50mL, uprightly discharge 1 day after, with bacterium bag handstand discharge water, make sack not have ponding, uprightly be emitted on the bedstead then, perhaps accumbency is on ground or bedstead, covered with plastic film is preserved moisture, and temperature is controlled at 13 ℃, and intensity of illumination is controlled at 5 luxs, after fruit body forms, take off and get plastic film;
(7) sporophore growth developing environment control: the bacterium bag that will form original hase or grow a large amount of young mushrooms uprightly is emitted on the bedstead, and temperature is controlled at 9 ℃, and intensity of illumination is controlled at 3 luxs, CO
2Concentration is controlled at 9000mg/kg, when fruit body grows into 6cm when high, put plastic sack on sack, the plastic sack specification is 18cm for the folding footpath, and length is 33cm, one end of plastic sack is fixed on the sack, open plastic sack and make it to be tubular, pricking sack, and stay a hole with the elastic ring, the aperture is 1cm, gathers when fruit body length reaches 16cm length.
Embodiment 3:A kind of vinasse cultivating flammulina velutipes method, it may further comprise the steps:
(1) medium preparation: culture medium prescription is vinasse 40%, cotton seed hulls 53% and wheat bran 7%, the culture medium preparation method is: the lime of adding 3.5% in vinasse, regulate the pH value to 6.5 of vinasse, mix by proportioning with cotton seed hulls, wheat bran then and mix thoroughly, add entry again, mix, make moisture content in medium reach 62%;
Vinasse are that the mixture of wheat and rice hulls is the vinasse that raw material forms.
(2) the bacterium bag is produced: with the medium polypropylene plastics pocket of packing into, the plastic sack specification is folding footpath 20cm, length 32cm, and inserting diameter at the pocket middle part then is 2cm, length is the 13cm sticking plaster, sets up the neck ring that diameter is 5cm at sack, seals with plastic film;
(3) sterilization: pocket is packed in the sterilization frame, and 6 in every frame charging bag is sterilized in autoclave cabinet, is sterilization treatment 3 hours under the 0.2 MPa pressure at pressure;
(4) inoculation: when pocket was cooled to 27 ℃, sterile working inserted bacterial classification, during inoculation sticking plaster in the pocket was taken out, and bacterial classification is put into bag and is pressed into the pocket middle part, covered the pocket surface simultaneously, and sack seals with aseptic paper;
(5) cultivate a bacterium: cultivating indoor cultivation, indoor temperature is controlled at 25 ℃, after the mycelial growth purseful, begins to enter management of producing mushroom;
(6) urge flower bud: change neck ring on the bacterium bag sack into into 7cm neck ring, dig out sack endosexine bacterial classification then, thickness is 1.5cm, add water then in bag, amount of water is 40mL, uprightly discharge 1 day after, with bacterium bag handstand discharge water, make sack not have ponding, uprightly be emitted on the bedstead then, perhaps accumbency is on ground or bedstead, covered with plastic film is preserved moisture, and temperature is controlled at 14 ℃, and intensity of illumination is controlled at 8 luxs, after fruit body forms, take off and get plastic film;
(7) sporophore growth developing environment control: the bacterium bag that will form original hase or grow a large amount of young mushrooms uprightly is emitted on the bedstead, and temperature is controlled at 10 ℃, and intensity of illumination is controlled at 4 luxs, CO
2Concentration is controlled at 8000mg/kg, when fruit body grows into 5cm when high, put plastic sack on sack, the plastic sack specification is 20cm for the folding footpath, and length is 40cm, one end of plastic sack is fixed on the sack, open plastic sack and make it to be tubular, pricking sack, and stay a hole with the elastic ring, the aperture is 1.5cm, gathers when fruit body length reaches 18cm length.
Embodiment 4:A kind of vinasse cultivating flammulina velutipes method, it may further comprise the steps:
(1) medium preparation: culture medium prescription is vinasse 50%, cotton seed hulls 45% and wheat bran 5%, the culture medium preparation method is: the lime of adding 4% in vinasse, regulate the pH value to 7 of vinasse, mix by proportioning with cotton seed hulls, wheat bran then and mix thoroughly, add entry again, mix, make moisture content in medium reach 63%;
Vinasse are that the mixture of iblet and rice hulls is the vinasse that raw material forms.
(2) the bacterium bag is produced: with medium pack into polypropylene plastics pocket or polyethylene plastic bag, the plastic sack specification is folding footpath 18cm, length 31cm, inserting diameter at the pocket middle part then is 2cm, length is the 14cm sticking plaster, sets up the neck ring that diameter is 5cm at sack, seals with plastic film;
(3) sterilization: pocket is packed in layer frame, and 6 in every layer of charging bag sterilized in the normal-pressure sterilization kitchen range, is 100 ℃ in temperature and handles 18 hours down;
(4) inoculation: when pocket was cooled to 28 ℃, sterile working inserted bacterial classification, during inoculation sticking plaster in the pocket was taken out, and bacterial classification is put into bag and is pressed into the pocket middle part, covered the pocket surface simultaneously, and sack seals with aseptic paper;
(5) cultivate a bacterium: cultivating indoor cultivation, indoor temperature is controlled at 20 ℃, and mycelial growth begins to enter management of producing mushroom apart from low 2 .5cm of bag;
(6) urge flower bud: change neck ring on the bacterium bag sack into into 7cm neck ring, dig out sack endosexine bacterial classification then, thickness is 2cm, add water then in bag, amount of water is 35mL, uprightly discharge 1 day after, with bacterium bag handstand discharge water, make sack not have ponding, uprightly be emitted on the bedstead then, perhaps accumbency is on ground or bedstead, covered with plastic film is preserved moisture, and temperature is controlled at 15 ℃, and intensity of illumination is controlled at 6 luxs, after fruit body forms, take off and get plastic film;
(7) sporophore growth developing environment control: the bacterium bag that will form original hase or grow a large amount of young mushrooms uprightly is emitted on the bedstead, and temperature is controlled at 5 ℃, and intensity of illumination is controlled at 5 luxs, CO
2Concentration is controlled at 7500mg/kg, when fruit body grows into 5cm when high, put plastic sack on sack, the plastic sack specification is 19cm for the folding footpath, and length is 35cm, one end of plastic sack is fixed on the sack, open plastic sack and make it to be tubular, pricking sack, and stay a hole with the elastic ring, the aperture is 2cm, gathers when fruit body length reaches 17cm length.
Claims (2)
1. vinasse cultivating flammulina velutipes method, it is characterized in that: it may further comprise the steps:
(1) medium preparation: culture medium prescription is vinasse 30～50%, cotton seed hulls 40～60% and wheat bran 5～10%, the culture medium preparation method is: add 3～4% lime in vinasse, regulate pH value to 6～7 of vinasse, mix by proportioning with cotton seed hulls, wheat bran then and mix thoroughly, add entry again, mix, make moisture content in medium reach 60～65%;
(2) the bacterium bag is produced: with medium pack into polypropylene plastics pocket or polyethylene plastic bag, the plastic sack specification is folding footpath 17～22cm, length 30～33cm, inserting diameter at the pocket middle part then is 2cm, length is 13～14cm sticking plaster, set up the neck ring that diameter is 5cm at sack, seal with plastic film;
(3) sterilization: with pocket pack into the sterilization frame in or the layer frame in, 6 in every frame or every layer charging bag, in autoclave cabinet or normal-pressure sterilization kitchen range, sterilize, when in autoclave cabinet, sterilizing, at pressure is sterilization treatment 3～4 hours under 0.1～0.2 MPa pressure, when in the normal-pressure sterilization kitchen range, sterilizing, be 98～100 ℃ in temperature and handled 18～20 hours down;
(4) inoculation: when pocket was cooled to below 28 ℃, sterile working inserted bacterial classification, during inoculation sticking plaster in the pocket was taken out, and bacterial classification is put into bag and is pressed into the pocket middle part, covered the pocket surface simultaneously, and sack seals with aseptic paper;
(5) cultivate a bacterium: cultivating indoor cultivation, indoor temperature is controlled at 19 ℃～25 ℃, and mycelial growth after the purseful of perhaps growing, begins to enter management of producing mushroom apart from the low 2～3cm of bag;
(6) urge flower bud: change neck ring on the bacterium bag sack into into 7cm neck ring, dig out sack endosexine bacterial classification then, thickness is 1～2cm, add water then in bag, amount of water is 30～50mL, uprightly discharge 1 day after, with bacterium bag handstand discharge water, make sack not have ponding, uprightly be emitted on the bedstead then, perhaps accumbency is on ground or bedstead, covered with plastic film is preserved moisture, and temperature is controlled at 10～15 ℃, and intensity of illumination is controlled at 5～10 luxs, after fruit body forms, take off and get plastic film;
(7) sporophore growth developing environment control: the bacterium bag that will form original hase or grow a large amount of young mushrooms uprightly is emitted on the bedstead, and temperature is controlled at 5～10 ℃, and intensity of illumination is controlled at 3～5 luxs, CO
2Concentration is controlled at 7000～9000mg/kg, when fruit body grows into 4～6cm when high, put plastic sack on sack, the plastic sack specification is 18～22cm for the folding footpath, and length is 33～45cm, one end of plastic sack is fixed on the sack, open plastic sack and make it to be tubular, pricking sack, and stay a hole with the elastic ring, the aperture is 1～2cm, gathers when fruit body length reaches 16～20cm length.
2. a kind of vinasse cultivating flammulina velutipes method according to claim 1, it is characterized in that: described vinasse are the mixture of wheat and rice hulls, or the mixture of iblet and rice hulls is the vinasse that raw material forms.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577839A (en) * | 2011-12-01 | 2012-07-18 | 浙江塔牌绍兴酒有限公司 | Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium |
| CN102584363A (en) * | 2011-12-01 | 2012-07-18 | 浙江塔牌绍兴酒有限公司 | Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium |
| CN103508798A (en) * | 2013-09-27 | 2014-01-15 | 合肥市天丰菌业科技有限公司 | Enokitake cultivation material using silkworm excrement as raw material and preparation method thereof |
| CN104151012A (en) * | 2014-06-09 | 2014-11-19 | 泰安生力源生物工程有限公司 | Method for preparing mushroom cultivation base material from fresh vinasse |
| CN104326840A (en) * | 2014-11-24 | 2015-02-04 | 王惠莹 | Needle mushroom culture medium and preparation method thereof |
| CN104838993A (en) * | 2015-04-20 | 2015-08-19 | 江苏华绿生物科技股份有限公司 | Distiller grain composite culture medium and application thereof in factory-like white needle mushroom cultivation |
| CN105638246A (en) * | 2016-02-03 | 2016-06-08 | 毕节市中药研究所 | Method for cultivating gastrodia-elata-armillaria-mellea production strains through vinasse |
| CN106631336A (en) * | 2016-09-28 | 2017-05-10 | 四川省农业科学院土壤肥料研究所 | Culture medium for high-yield cultivation of flammulina velutipes and preparation method of culture medium |
| CN110337987A (en) * | 2019-07-31 | 2019-10-18 | 重庆市大足区陈氏食用菌股份合作社 | A kind of golden mushroom plantation method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577839A (en) * | 2011-12-01 | 2012-07-18 | 浙江塔牌绍兴酒有限公司 | Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium |
| CN102584363A (en) * | 2011-12-01 | 2012-07-18 | 浙江塔牌绍兴酒有限公司 | Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium |
| CN102577839B (en) * | 2011-12-01 | 2013-07-31 | 浙江塔牌绍兴酒有限公司 | Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium |
| CN102584363B (en) * | 2011-12-01 | 2013-11-27 | 浙江塔牌绍兴酒有限公司 | Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium |
| CN103508798A (en) * | 2013-09-27 | 2014-01-15 | 合肥市天丰菌业科技有限公司 | Enokitake cultivation material using silkworm excrement as raw material and preparation method thereof |
| CN104151012A (en) * | 2014-06-09 | 2014-11-19 | 泰安生力源生物工程有限公司 | Method for preparing mushroom cultivation base material from fresh vinasse |
| CN104326840A (en) * | 2014-11-24 | 2015-02-04 | 王惠莹 | Needle mushroom culture medium and preparation method thereof |
| CN104838993A (en) * | 2015-04-20 | 2015-08-19 | 江苏华绿生物科技股份有限公司 | Distiller grain composite culture medium and application thereof in factory-like white needle mushroom cultivation |
| CN105638246A (en) * | 2016-02-03 | 2016-06-08 | 毕节市中药研究所 | Method for cultivating gastrodia-elata-armillaria-mellea production strains through vinasse |
| CN106631336A (en) * | 2016-09-28 | 2017-05-10 | 四川省农业科学院土壤肥料研究所 | Culture medium for high-yield cultivation of flammulina velutipes and preparation method of culture medium |
| CN110337987A (en) * | 2019-07-31 | 2019-10-18 | 重庆市大足区陈氏食用菌股份合作社 | A kind of golden mushroom plantation method |
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Application publication date: 20110119 |