KR20150001859U - Complete culture hardwood medium of Poria cocos cultivated after inoculating spawn to the pine hardwood - Google Patents

Abstract

The present invention relates to a cultivated woody medium for the cultivation of bamboo shoots cultivated after inoculation with bamboo shoots in a pine tree of 10 to 17 cm in diameter and 17 to 23 cm in length, It is possible to reduce the damage caused by pollution, and it is possible to increase the efficiency of cultivation by making it possible to use the idle forest instead of the facility as the cultivation area, and it shows excellent productivity in the sawdust medium, .

Description

{Complete culture hardwood medium of Poria cocos cultivated after inoculating the pine tree seedlings,

The present invention relates to a cultivated woody medium for cultivation of bamboo shoots cultivated after inoculation of bamboo seedlings into pine trees.

Fungi are a class of organisms and consist of yeast, fungi, and mushrooms. Higher plants that have been included in plants in the past to photosynthesize, and silver plants that do not photosynthesize against algae, such as fungi, fungi, fungi and the like. Recently, it has been classified as a systematic group of organisms which is fundamentally different from plants by regeneration, reproduction method, biochemical characteristics, and the like. The fungi are characterized in that there are no anabolic pigments such as chlorophyll in all of them. Therefore, it does not produce nutrients by itself because it is photosynthetic like high plants, so it attaches to other living organisms or organic matter and parasitic or byproducts, and most of the nutrients are hyphae.

The basidiomycetes belong to the family of the fungus, and contain so-called mushrooms. In the fungus, it accounts for about 30% after the fungus. The body consists of tubular cells called many mycelium, forming mostly fleshy fruiting bodies.

Mushrooms are the microorganisms belonging to the bacterium belonging to the organism classification system, and they are mostly used for food, except that they are toxic. These mushrooms are expected to be high-protein foods as a substitute for future meat. Especially, according to various recent research reports, it is known that glucan and its derivatives contained in mushrooms have anticancer activities. It can be seen that it is being developed as health food as well as use.

Mushrooms are cultivated and cultivated under different conditions. Mushrooms are usually cultivated by inoculating mushroom seedlings into logs or mushroom culture medium.

Mushroom seeds (spawn) are seeds for mushroom cultivation and are called seeds, including cultured cells and medium. The mushroom seeds are classified into solid form and liquid form according to the material and form of the medium used in general, sawdust seeds in solid form, and liquid seeds in liquid form. Sawdust seeds are commonly used in the cultivation of boxing mushroom, oyster mushroom, top mushroom, shiitake mushroom, gingko mushroom and woody mud mushroom, box cultivation, encapsulation cultivation, . When solid mushroom seeds with solid form are inoculated into mushroom culture medium, the mushroom seedlings are inoculated near the surface of the culture medium. Since the mushroom culture is not inoculated deep into the culture medium, it takes a long time to transfer the mushroom bacteria to the culture medium. The mushroom cultivation period becomes longer.

Liquid seedlings are cultivated with mushroom seedlings in liquid nutrient solution. The production period of the seedlings is shorter than the production of solid seedlings such as sawdust seedlings, and can be manufactured in large quantities, but it has difficulties in aseptic manipulation techniques. In addition, when the mushroom seeds having a liquid form are inoculated into the mushroom culture medium and germinating the mushroom seeds, the mushroom seedlings are in a liquid state, so that the cultivation period of the mycelium becomes long due to excessive moisture in the medium. In addition, when the mushroom seedlings are inoculated into the mushroom culture medium, the seedlings are inoculated near the surface of the medium, and it takes a long time for the seedlings to spread to the depth of the medium.

Boryeong (伏 笭: Poria cocos ) is a type of mushroom, which is classified as bacillus, and is a double-ring type belonging to the mushroom family. It is round or elongated sclerotial parasitism parasitized on the roots of the pine tree. It is reddish brown or blackish brown on the surface. It is called myeongryeong mushroom or myeongryeong. In Oriental medicine, it is divided into Baekbokryeong, Baekbokryeong, Bokseong, and Byeongryeok (bark). The native areas of Byeongryong can be seen in North America, but the main regions are mostly distributed in Southeast Asia including Korea. According to the choice of sclerotia, bokyong can be divided into Baekbokgyeong and Baekbokyeong, and many varieties can be seen depending on the area where it is wild. Among them, not only the intensity of mycelial growth in artificial cultivation is different but also the quality and the yield of cultivation are different. Therefore, when artificial cultivation is attempted for mass production, it is very important factor to determine the success or failure according to the characteristics of the strain.

Bepyeong has been widely used as a herbal medicine ingredient since ancient times, and its ancestors have widely used in the daily life such as Bokryeongjae, Bokryeokguksuo, Bokryeongcha, Bokryongju, and skin beauty. It was used as a precious herbal medicine and herbal medicine so that there was a record of the old document that it is spiritual if it takes long time for bokryeong. It is buried in the basement, and when it is picked, it uses a skewer to pierce the ground deeply. The consumption of natural herb has been decreasing in recent years, but the consumption of herbal medicine has continued to increase. Because of this disparity in demand and supply, most of the ginseng consumed in the Chinese herbal medicine market is dependent on imports from foreign countries such as China (underground cultivation bell), and in recent years it has been used to make ginseng as food, cosmetics, Attempts are increasing. In particular, bark (bark) has been found to contain a large amount of physiological activating factors in recent studies, and active research is underway.

Bokseong is the whole of the pine root, which is parasitic to the dead pine root, and the pine root in the bokryeong is partly differentiated from the pine root. In recent years, as it is difficult to artificially cultivate the ginseng, it is possible to distribute the ginseng as a whole.

In general, the cultivation of Bokryeong mushroom (Bokyeong) and Bokseong was carried out by cultivation method (refer to Fig. 1) in which pine trees were buried in the soil together with Bokyeong seedlings in Korea in the 1990s, See Fig. 2), which has been made up of ground cultivation in a facility house or an existing mushroom grower. For this purpose, not only pine but also various wood such as conifer and hardwood are cut to 20 to 30 cm, then layered or poured into a plastic bag to inoculate and deposit the seed bacterium using sawdust or corn- It is necessary to manage the aging and growth stages without removing or removing the seeds used in the inoculation without removing or removing the vinyl that has been blanketed in the room or the room of the facility, It maintains temperature and humidity, and it is a method to cultivate clean bokyu and bokhin which does not contain pesticides or heavy metals in the inside of the facility or on the ground. This is a way to produce bokyong and bokhin on the ground without burying the wood after the inoculation. However, the above method has disadvantages such as damage due to contamination due to non-sterilization, and additional efficiency of facility production due to the additional cost of the facility.

Accordingly, the present inventor has made efforts to develop a medium for cultivation of bamboo shoots using a new cultivated woody medium which is superior to the conventional mushroom seedlings in efficiency of seedling administration and excellent efficiency of mushroom fungus germination in the medium. As a result, It is advantageous to reduce the damage caused by soil contamination by using the cultured finished wood medium after inoculation of the seedlings at 17 cm to 23 cm in length and cultivating the cultivation area by using the idle forest rather than the facility, The present invention has been completed by confirming that it can be effectively used as a bacterium for the cultivation of bamboo seeds using a cultivated woody medium for cultivation of baeobyeong mushroom which can improve efficiency and exhibit excellent productivity in sawdust bag medium.

The object of the present invention is to provide a cultivated wood medium cultured after inoculation with a bamboo shoot seedlings in a pine (chrysanthemum) tree having a diameter of 10 cm to 17 cm and a length of 17 cm to 23 cm.

In order to solve the above-mentioned object, the present invention provides a culture medium for cultivation of bamboo shoots cultivated after inoculation with bamboo shoots in a pine tree (chestnut tree) having a diameter of 10 cm to 17 cm and a length of 17 cm to 23 cm.

The present invention also relates to a method for producing a heat-resistant plastic bag, comprising the steps of: 1) placing a pine tree (log) wood having a diameter of 10 cm to 17 cm and a length of 17 cm to 23 cm into a heat-resistant plastic bag;

2) placing the logs of step 1) in a autoclave and sterilizing;

3) cooling the sterilized log of step 2) in a clean room;

4) inoculating the cooled seedlings of step 3) with the bacterium seedlings; And

And 5) culturing the inoculated cultured wood medium of step 4). The present invention also provides a method of cultivating the cultured medium.

The present invention relates to a cultivated woody medium for cultivation of bamboo shoots cultivated after inoculation of bamboo shoots in the pine trees, and by using the cultivated woody medium, it is possible to reduce the damage caused by pollution when the soil is buried, The use of idle forests has the characteristic of enhancing the efficiency of cultivation.

FIG. 1 is a view showing a method of cultivating a pine tree, which is one of the methods of artificial cultivation in Korea, along with a bryophyte.
FIG. 2 is a view showing a method of cultivating non-reclamation rice gardens using a plastic bag, which is one of the conventional artificial cultivation methods in Korea.
Fig. 3 is a graph showing the results obtained by cutting a green lumber at a diameter of 12 cm to 15 cm and a length of 20 cm into a heat-resistant plastic bag, sterilizing the lumber at 121 캜 for 90 minutes, And then incubated for 30 to 45 days in a culture room at 20 to 25 DEG C for 30 to 45 days. Then, the seeds are buried in idle forests in about 3 to 4 months, Fig.

Hereinafter, the present invention will be described in detail.

The present invention provides a cultivated woody medium for cultivation of bamboo shoots cultured after inoculation of bamboo seedlings into a pine tree (jangsong) having a diameter of 10 cm to 17 cm and a length of 17 cm to 23 cm.

The above-mentioned culture medium for cultivation of bamboo shoots comprises the steps of (1) placing a pine (chrysoptera) wood having a diameter of 10 cm to 17 cm and a length of 17 cm to 23 cm into a heat resistant plastic bag;

2) placing the logs of step 1) in a autoclave and sterilizing;

3) cooling the sterilized log of step 2) in a clean room;

4) inoculating the cooled seedlings of step 3) with the bacterium seedlings; And

5) culturing the inoculated cultured wood medium of step 4), but it is not limited thereto.

It is preferable that the pine of step 1) is any one of the South pine tree, the gold pine tree, the pine tree, the pine tree, the pine tree, and the pine tree.

In addition, the pine can be used without being specifically cultivated or commercially available.

The sterilization of step 2) is preferably sterilized at 110 to 125 ° C for 70 to 110 minutes, more preferably at 119 to 123 ° C for 80 to 100 minutes, but is not limited thereto.

By the above-mentioned sterilization, it is possible to reduce the damage caused by pollution caused by non-sterilization which is a disadvantage of the traditional method of cultivation which has been used in Korea.

The cooling in step 3) is preferably carried out at 10 to 20 ° C for 180 minutes to 300 minutes, more preferably at 12 to 18 ° C for 200 to 280 minutes, but is not limited thereto.

The bacterium of step 4) is preferably a bacterium or a bacterium. The bacterium solid seed bacterium is preferably a bacterium sawdust bacterium, a bacterium cereal bacterium or a bacteriostatic horse seed bacterium, and most preferably a bacterium sawdust bacterium, but is not limited thereto.

In addition, the bacterium is preferably used for cultivation of Bokryeong (mackerel mushroom) or bacillus, but is not limited thereto.

The inoculation of step 4) is preferably inoculated outside or inside the pine tree, more preferably, but not necessarily, inoculated into the pine tree.

The cultured medium log of step 5) is preferably sawdust medium or grainy medium, but is not limited thereto.

The culture of step 5) is preferably cultivated for 30 to 45 days in an incubation room at 20 to 25 DEG C, more preferably for 35 to 45 days in an incubation room at 20 to 24 DEG C, It does not.

In addition, the present invention provides a cultivation method of bokryeong cultivated for 12 to 36 months after being buried in an idle forest using the above-mentioned cultured medium for cultivation of bokryeong.

Since there is no additional cost of the facility through the cultivation in the above-mentioned idle forest, there is an advantage that the economical and industrial efficiency of the production is increased.

In a specific example, the present inventors used bamboo shoot seedlings having a diameter of 12 cm to 15 cm and a length of 22 cm to inoculate bamboo seedlings for about 6 months using seedling broths cultured for 43 days, As a result, it was confirmed that 52 g of Bokryeong was harvested.

Therefore, the culture medium after culturing the bamboo shoots in the pine trees of 10 to 17 cm in diameter and 17 to 23 cm in length has been used in Korea through the sterilization step It is possible to reduce the damage caused by pollution caused by non-sterilization which is a disadvantage of the whole bamboo cultivation method, and it is possible to cultivate in the idle forest, so that there is no additional cost of the facility, It can be used as a medium.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the content of the present invention is not limited to the following examples.

< Example  1> Bacillus cultivation  Cultivation completed Logwood  Produce

Gyeongbuk Province Gyeongju-si The carpis was cut to a diameter of 12 cm to 15 cm and a length of 20 cm to 22 cm. Then, the high-pressure sterilizer and air in the medium were removed by opening the exhaust valve when the pressure was about 0.7 kg / cm ³ . Then, the to close the exhaust valve, and gradually pressure 1.0 kg / cm ³ To 1.2 kg / cm &lt; ^{3 &} gt ;, allowing the temperature to reach 121 DEG C and sterilization for 90 minutes. During sterilization, the pressure in the high-pressure sterilizer continued to rise, so the exhaust valve was occasionally opened to maintain the pressure at 1.2 kg / cm ³ . After the sterilization was finished, the pressure in the high pressure sterilizer was gradually lowered, and when the needle of the pressure gauge was pointing to zero, the door was opened to take out the spike. The sterilized sorghum was cooled from room temperature to about 15 ° C for 180 minutes to 300 minutes. The bactericidal seeds were inoculated with bivalve sawdust seeds (MKACC 04790) and then cultured in a culture room at 20 to 25 캜 for 30 to 45 days.

< Example  2> Bacillus cultivation  Cultivation completed Logwood badge  Survey of production volume of bamboo cultivated by using

The following experiment was carried out to investigate the yield of cultivated gyohyeong grown using the gyoza cultured medium for bamboo shoot cultivation prepared in <Example 1>.

Specifically, we used a general vinyl house grower located in Soseong - gun, Kyungsangbuk - do to produce. The cultivated woody medium for bamboo shoot cultivation produced in Example 1 was buried in idle forest in April 2012 and cultivated with bamboo shoots and harvested in October 2013.

As a result, as shown in Table 1, the medium (mycelial density: +++) cultured for 43 days in the culture room was buried in the idle forest in April 2012 and cultivated for 18 months, The weight of the bamboo was 52 g.

Claims (9)

A cultivated woody medium for cultivation of bamboo shoots cultivated after inoculation of bamboo seedlings into a pine (jangsong) log of 10 to 17 cm in diameter and 17 to 23 cm in length.
[Claim 3] The log leaf as set forth in claim 1, wherein the pine tree is any one of an Abyssinian pine, a golden pine tree, a pine tree, a pine tree, a pine tree, a pine tree,
[Claim 3] The culture medium of claim 1, wherein the bacterium is a bacterium sawdust seedling.
[Claim 2] The method according to claim 1, wherein the bacterium is used for cultivation of bokryeong (mackerel mushroom) or bacillus.
[Claim 5] The culture medium of claim 1, wherein the culture medium is a sawdust medium or a ground medium.
The method according to claim 1, wherein the culture medium
1) placing a pine tree (log) wood having a diameter of 10 cm to 17 cm and a length of 17 cm to 23 cm into a heat-resistant plastic bag;
2) placing the logs of step 1) in a autoclave and sterilizing;
3) cooling the sterilized log of step 2) in a clean room;
4) inoculating the cooled seedlings of step 3) with the bacterium seedlings; And
And 5) culturing the inoculated cultured wood medium of step 4).
[Claim 7] The method according to claim 6, wherein the sterilization of step 2) is performed at 110 [deg.] C to 125 [deg.] C for 70-110 minutes.
The method according to claim 6, wherein the cooling of step 3) is performed at a temperature of 10 to 20 DEG C for 180 to 300 minutes.
[Claim 7] The method according to claim 6, wherein the culture of step 5) is cultivated for 30 days to 45 days in a culture room at 20 [deg.] C to 25 [deg.] C.

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