KR20200074625A - Method for preparing sawdust media for cultivating poria cocos spawn using tree died by pine wilt disease - Google Patents

Abstract

The present invention relates to a manufacturing method of sawdust medium for cultivating Poria cocos spores using dead trees died of pine wilt disease. More specifically, the present invention relates to a manufacturing method of sawdust medium for cultivating Poria cocos spores using dead trees died of pine wilt disease. Since the sawdust medium for cultivating Poria cocos spores can be prepared using a discarded dead trees died of pine wilt disease, it is possible to improve the economic feasibility and increase efficiency of cultivation of Poria cocos spores by performing heat treatment after chemical treatment using peracetic acid on sawdust obtained from dead trees died of pine wilt disease.

Description

Method for preparing sawdust media for cultivation of Bokryeong spp. using pine tree nematode disease

The present invention relates to a method for manufacturing a sawdust medium for cultivation of Bokryeong seed germ using pine tree nematode disease, and more specifically, to manufacture a sawdust medium for cultivation of Bokryeong seed germ tree using discarded pine tree nematode disease. For the manufacturing method of sawdust medium for cultivation of cultivation of cultivation of bokryeong seed using pine wood worm disease, which can improve the efficiency of cultivation of cultivar wormworm by performing heat treatment after chemical treatment with peracetic acid for sawdust obtained from dead wood of pine wilt disease will be.

Pine is an evergreen coniferous tree of the coniferous tree, the pine family. It grows all over Korea except for the northern highlands, and suffers much damage from pine nematode disease. Pine nematode disease is a disease caused by pine nematodes, which are parasites that eat parasites and gnaw trees, and the pine nematodes parasitize on the larvae, such as pine brine, and beard beetle, and are transferred to the trees through the vector. However, pine wilt disease has no treatment method so far, and the infection can be confirmed only with the naked eye, so many pine trees are killed by pine wilt disease. Therefore, various methods have been tried to use the pines killed by the pine wilt disease, and in general, the pine wilt disease is killed by using heat treatment of the pine wilt disease, as in the following patent document, and then the firewood is used.

<Patent Document>

Published Patent Publication No. 10-2009-0011397 (published on February 02, 2009) "How to recycle pine tree nematode disease and suspected trees"

However, in the conventional method of treating the dead tree disease of pine wilt disease, there is a problem that the use of dead tree after heat treatment is limited by focusing only on killing the pine tree nematode through heat treatment. In particular, there is a problem in that it is not possible to cultivate Bokryeong using pine tree nematode disease.

The present invention is to solve the problems of the prior art described above,

The present invention is to provide a method for producing a sawdust medium for cultivation of a wormwood seedworm using pinewood wilt disease, which can improve economic efficiency, by using a discarded pine woodworm beetle rotwood tree to improve economic efficiency. There is this.

In addition, the present invention, by performing a heat treatment after the chemical treatment with peracetic acid for sawdust obtained from pine tree nematode disease dead tree, by effectively removing the fungus that inhibits the growth of pine nematodes and pufferia, it is possible to improve the efficiency of cultivation of pufferworm seed germ. It relates to a method of manufacturing a sawdust medium for cultivation of Bokryeong spawn using dead pine tree nematode disease.

The present invention is implemented by an embodiment having the following configuration in order to achieve the above object.

According to an embodiment of the present invention, the method of manufacturing a sawdust medium for cultivation of Bokryeong seed germ according to the present invention includes a sawdust harvesting step of crushing the dead tree of pine wilt disease to obtain sawdust, and a heat treatment step of heat treatment by applying heat to the sawdust It is characterized by.

According to another embodiment of the present invention, a method of manufacturing a sawdust medium for cultivation of bokryeong spawn according to the present invention includes a chemical treatment step of processing a dilute peracetic acid in sawdust obtained in the sawdust harvesting step, and sawdust after the chemical treatment step. A drying step of removing the peracetic acid diluting liquid remaining in the sawdust by removing it from the acetic acid diluent and drying it is further included. The heat treatment step is characterized in that heat is applied to the sawdust dried in the drying step.

According to another embodiment of the present invention, in the method of manufacturing sawdust medium for cultivation of Bokryeong spawn according to the present invention, the pine tree nematode disease is characterized by secondary infection by fungi.

According to another embodiment of the present invention, in the method of manufacturing sawdust medium for cultivating bokryeong spawn according to the present invention, the peracetic acid diluent is mixed with peracetic acid in water, and a dilute peracetic acid solution having a concentration of 0.8 to 1.2% is used. It is characterized by being.

According to another embodiment of the present invention, in the method of manufacturing sawdust medium for cultivation of Bokryeong spawn according to the present invention, the chemical treatment step is characterized in that sawdust is immersed in a dilute peracetic acid solution for 25 to 35 seconds.

According to another embodiment of the present invention, in the manufacturing method of the sawdust medium for cultivation of Bokryeong seeds according to the present invention, the heat treatment step is characterized in that it is performed by applying high pressure steam to the dried sawdust.

According to another embodiment of the present invention, in the method of manufacturing a sawdust medium for cultivation of Bokryeong seeds according to the present invention, the heat treatment step is performed by adding high pressure steam to the dried sawdust and heat-treating it at 115 to 130° C. for 70 to 120 minutes. , After heat treatment, it is characterized in that the water content of sawdust has 50 to 70%.

According to another embodiment of the present invention, the method of cultivation of Bokryeong according to the present invention is inoculated with a sawdust medium in a sawdust medium and maintained at a temperature of 24 to 28°C for 2 to 3 weeks to form a sawdust culture. And a lumber formation step of forming a lumber for inoculating sawdust cultivation by heat-treating and treating the pine tree nematode disease chemical tree, and a cultivation cultivation step of inoculating sawdust cultivation in the lumber and culturing to produce bokyeong. The sawdust badge is characterized in that the sawdust badge manufactured by the method of any one of claims 3 to 7 is used.

According to another embodiment of the present invention, in the cultivation method of Bokryeong according to the present invention, the tree formation step is a chemical treatment step of processing a dilute peracetic acid solution on a tree obtained from a pine tree nematode disease, and a chemical treatment in the chemical treatment step And a drying step of drying the treated wood to remove the peracetic acid diluent remaining in the wood, and a heat treatment step of applying heat to the dried wood in the drying step, wherein the pine wilt disease dead wood is molded by fungi. It is characterized by being infected with tea.

According to another embodiment of the present invention, in the method for cultivating bokryeong according to the present invention, the wood-forming step is prepared by mixing the peracetic acid with water to prepare a dilute peracetic acid to have a concentration of 0.8 to 1.2%. And, the wood is immersed in the peracetic acid dilution for 25 to 35 seconds, or the wood is sprayed with the dilute peracetic acid for 25 to 35 seconds, and the heat treatment step is performed by applying high-pressure steam to the dried wood to 115 to 130 It is characterized in that it is carried out by heat treatment for 100 to 150 minutes at ℃.

The present invention can obtain the following effects by the present embodiment.

The present invention can produce a sawdust medium for cultivation of Bokryeong spawn using dead pine tree nematode disease, which can improve the economic efficiency.

In addition, the present invention, by performing a heat treatment after chemical treatment with peracetic acid on sawdust obtained from pine tree nematode disease dead tree, by effectively removing the fungus that inhibits the growth of pine nematodes and pufferia, it is possible to improve the efficiency of cultivation of pufferworm spp. It has an effect.

1 to 3 is a picture for confirming the degree of contamination of the sawdust badge produced by the method of manufacturing a sawdust badge according to an embodiment of the present invention.
4 to 6 is a picture for confirming the degree of contamination of the wood produced by the wood manufacturing method according to another embodiment of the present invention.
Figure 7 is a photograph showing the appearance of the bokryeong spawn in the wood produced by the method of manufacturing wood according to another embodiment of the present invention.
Figure 8 is a photograph showing a cultivated bokyeong by the cultivation method of bokryong according to another embodiment of the present invention.

Hereinafter, a method of manufacturing sawdust medium for cultivation of Bokryeong spores using pine tree nematode disease according to the present invention and a method of cultivating Bokryeong using sawdust medium produced by the above-described manufacturing method will be described in detail with reference to the accompanying drawings. Unless otherwise specified, all terms in this specification are the same as the general meaning of the term understood by a person skilled in the art to which the present invention belongs, and if there is a conflict with the meaning of the term used in this specification, It follows the definition used in the specification. In addition, detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention are omitted. Throughout the specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated.

Method of manufacturing a sawdust medium for cultivation of Bokryeong seed germ using pine tree nematode disease according to an embodiment of the present invention is to form a sawdust medium for cultivation of Bokryeong seed germ by chemically treating the sawdust obtained from dead pine tree nematode treatment It includes the obtaining step, the chemical treatment step, the drying step, the heat treatment step, and the mixing step. The pine tree nematode disease dead tree refers to a pine tree that has been cut off by pine tree nematode disease, and is mostly secondaryly infected by fungi such as blue mold and Fusarium. The bokryeong spontaneously grows from dead pine trees. When the common pine tree nematode dead tree is used by simple heat treatment as in the prior art, it is possible to kill the pine wiltworm, but the cultivation of the bokryeong is impossible, and the present invention is secondary infection by fungi. It was completed to enable efficient cultivation of Bokryeong using the common pine wilt disease, and will be described in detail below.

The sawdust harvesting step is a step of obtaining sawdust by crushing the dead tree of pine wilt disease, and a conventional method of obtaining sawdust by crushing wood may be used.

The chemical treatment step is a step of processing a peracetic acid diluent in the sawdust obtained in the sawdust harvesting step (for example, immersing the sawdust in a dilute peracetic acid for a certain time), mixing peracetic acid with water to increase the concentration of 0.8 to 1.2%. A dilute peracetic acid solution is used and is preferably immersed for 25 to 35 seconds.

The drying step is a step of removing the sawdust from the dilute peracetic acid after the chemical treatment step and drying it, for example, may be performed by drying for 160 to 200 minutes using an oven set at 50°C. If the sawdust is not properly dried in the drying step, a dilute peracetic acid remains in the sawdust, thereby inhibiting the culture of the bokryeong spawn.

In the heat treatment step, heat is applied to the sawdust dried in the drying step, and high-pressure steam is added to the dried sawdust to heat treatment at 115 to 130° C. for 70 to 120 minutes, and the water content of sawdust after heat treatment is 50 to 70. It is desirable to have %. In the heat treatment step, the heat treatment is performed using steam, so that sawdust can have a water content capable of cultivating the bokryeong spawn. The heat treatment step may be performed using an apparatus such as an autoclave.

The mixing step is a step of forming a sawdust medium by mixing rice flour, calcium, and the like with the sawdust heat-treated in the heat treatment step. It is possible to kill the pine wilt disease by performing the heat treatment step directly on the sawdust obtained by crushing the pine wilt disease, but the present invention is not capable of cultivation of the Bokyeong spp. After treatment and drying, heat treatment is performed to obtain a sawdust medium capable of cultivating Bokryeong spawn.

Another embodiment of the present invention relates to a sawdust medium produced by a method of manufacturing a sawdust medium for cultivation of Bokryeong spawn using pine wilt disease.

Referring to FIGS. 1 to 8, a method of cultivating bok-ryeong using sawdust medium prepared by a method of manufacturing sawdust medium for cultivation of bok-ryeong seeds using pine tree nematode disease according to another embodiment of the present invention will be described. The method of cultivation is the seeding culture step of inoculating the sawdust medium after inoculating the seed germ seed, and then cultivating the sawdust culture to form sawdust cultures, and the heat treatment after chemical treatment of the pine tree wilt disease dead wood to form the seedlings for inoculating the sawdust cultures. , Bokryeong culture step of inoculating sawdust cultivation seed in the tree and then incubating to produce Bokryeong. The sawdust badge is a sawdust badge prepared by a method of manufacturing a sawdust medium for cultivation of Bokryeong spp. using the pine tree nematode dead tree described above, so a detailed description thereof will be omitted.

The seeding culture step is a step of inoculating the sawdust medium after inoculating the Bokryeong seed germ to form a sawdust culture seed, and a conventional method of inoculating and culturing the sawdust medium obtained from dead pine can be used. For example, the sawdust medium can be placed in a container and liquid bokryeong seeds are added and maintained at a temperature of 24 to 28° C. for 2 to 3 weeks to form sawdust cultures.

The lumber formation step is a step of forming a lumber for inoculating sawdust cultivation by chemically treating the pine tree nematode disease dead wood, and includes a raw material harvesting step, a chemical treatment step, a drying step, and a heat treatment step.

The step of acquiring wood is a step of obtaining wood having a certain diameter and length by cutting dead pine trees.

The chemical treatment step may be performed by treating the raw wood obtained in the wood harvesting step with a peracetic acid diluent, for example, spraying the peracetic acid diluent on the wood or immersing the raw material in a peracetic acid diluent for a certain period of time, and peracetic acid Dilute peracetic acid to have a concentration of 0.8 to 1.2% by mixing with water, and it is preferable to spray or immerse for 25 to 35 seconds.

The drying step is a step of drying the wood chemically treated in the chemical treatment step, for example, it may be performed by drying for 160 to 400 minutes using an oven set at 50°C. If the wood is not properly dried in the drying step, a dilute peracetic acid remains in the wood, thereby inhibiting the cultivation of the puffer fish.

The heat treatment step is a step of applying heat to the dried wood in the drying step, and it is preferable to heat the steam at 115 to 130° C. for 100 to 150 minutes by adding high-pressure steam to the dried wood. In the heat treatment step, heat treatment is performed using steam, so that the wood can have a moisture content capable of cultivating bok-ryeong. The heat treatment step may be performed using an apparatus such as an autoclave. It is possible to kill the pine wilt disease by performing the heat treatment step directly on the wood obtained by cutting the pine wilt disease, but the present invention is impossible to grow pine trees. Then, heat treatment is performed to obtain a lumber capable of cultivation in Fueryeong.

The cultivation step is a step of inoculating the wood obtained in the wood formation step and inoculating the sawdust culture obtained in the seed culture step, followed by cultivation to produce bokyeong, and inoculating the wood from the dead pine tree and inoculating the wood with sawdust culture. Method of can be used. For example, the whole rice cultured seed can be inoculated into a tree and maintained at a temperature of 24 to 28° C. for 5 to 7 months to obtain bokryeong.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for explaining the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of sawdust samples

1. Sawdust is obtained by crushing the dead tree of pine wilt disease in which blue mold is growing, and the sawdust is immersed in a 1% peracetic acid solution for 30 seconds, then taken out and dried for 180 minutes using an oven set at 50°C. The sawdust was treated with high pressure steam at 121°C for 1 hour and 30 minutes using an autoclave to obtain sawdust sample 1.

2. Sawdust is obtained by crushing the dead tree of the pine wilt disease in which blue mold is growing, and the sawdust is immersed in a 1% peracetic acid solution for 30 seconds, then taken out and used autoclave for 1 hour at 121°C under high pressure steam. After processing for 30 minutes, sawdust sample 2 was obtained.

3. Sawdust is obtained by crushing the dead tree of pine wilt disease where blue mold is growing, and the sawdust is heat-treated at 121°C for 1 hour and 30 minutes with high pressure steam using an autoclave, and the heat-treated sawdust is 1% and After immersing in an aqueous acetic acid solution for 30 seconds, it was taken out and dried for 180 minutes using an oven set at 50°C to obtain sawdust sample 3.

4. Sawdust sample 4 was obtained under the same conditions as in Example 1, except that a 0.5% aqueous peracetic acid solution was used instead of a 1% aqueous peracetic acid solution.

5. Sawdust sample 5 was obtained under the same conditions as in Example 1 except that a 2% aqueous peracetic acid solution was used instead of a 1% aqueous peracetic acid solution.

6. Sawdust sample 6 was obtained under the same conditions as in Example 1, except that a 0.3% aqueous hydrogen peroxide solution was used instead of a 1% aqueous peracetic acid solution.

7. Sawdust obtained by crushing a normal pine tree (not affected by pine tree nematode disease and not contaminated with green mold) was treated with high pressure steam at 121°C for 1 hour and 30 minutes using an autoclave to obtain sawdust sample 7. .

8. Sawdust was obtained by crushing the dead wood of the pine wilt disease where green mold was growing, and the sawdust was used as sawdust sample 8.

9. Sawdust is obtained by crushing the dead tree of pine wilt disease in which blue mold is growing, and the sawdust is immersed in a 1% peracetic acid solution for 30 seconds, then taken out and dried for 180 minutes using an oven set at 50°C. Sample 9 was obtained.

10. Sawdust was obtained by crushing the dead tree of the pine wilt disease in which blue mold is growing, and the sawdust was treated with autoclave at 121°C for 1 hour and 30 minutes to obtain sawdust sample 10.

<Example 2> Confirmation of contamination of sawdust sample

1. Put 10 ml of each sawdust sample obtained in Example 1 and 30 ml of sterile water into a 50 ml conical tube, vortex and apply 200 µl of the supernatant to potato agar medium (PDA), after 7 days, photographed in FIGS. 1 to 3 Table 1 shows the use of sawdust sample 1 in FIG. 1, sawdust sample 7 in FIG. 2, and sawdust sample 10 in FIG. 3, and also records the type of colony growing, the number of colonies, and the area of contamination. It is shown in.

2. Referring to FIGS. 1 to 3 and Table 1, while the chemical treatment and heat treatment using a 1% peracetic acid solution for sawdust obtained from the pine wilt diseased tree with blue mold growing, while contamination does not occur, chemical treatment B. When only one of the heat treatments is performed or the concentration of the aqueous peracetic acid solution is low, it can be confirmed that contamination occurred.

Colony type Colony Count Contaminated area (%) Sawdust Sample 1 0 0 0 Sawdust Sample 2 0 0 0 Sawdust Sample 3 0 0 0 Sawdust Sample 4 One 2 6 Sawdust Sample 5 0 0 0 Sawdust Sample 6 0 0 0 Sawdust Sample 7 0 0 0 Sawdust Sample 8 2 10 100 Sawdust Sample 9 One 5 30 Sawdust Sample 10 One 4 15

<Example 3> Culture of Bokryeong seeds using sawdust samples

1. After mixing 2 cm 3 of heat-treated rice bran per 10 cm 3 of each sawdust sample, 0.03 g of Ca, put it in a container, inoculate 0.07 g of liquid Pyeongnyeong spawn, incubate at 26° C. for 7 days, and confirm the degree of spawning. It is shown in Table 2. If nothing grows on the sawdust sample, it is evaluated as X, and it is evaluated as 1 to 5 according to the ratio of the fungus seed germ and other fungi growing on the sawdust sample, and it is evaluated as 1 if only the germ germ is grown, and 2 if the germ germ grows more than other fungi. , It was evaluated as 3 if the fungus was different from the other fungi, 4 if the fungus was less than the other fungus, and 5 if the fungus was only grown.

2. Referring to Table 2, in the case of heat treatment after chemical treatment with 1% peracetic acid solution for sawdust obtained from the pine wilt disease of pine wilt disease, where blue mold grows, the Bokryeong spawn grows well, but does not undergo drying after chemical treatment. It can be seen that when using a high concentration of peracetic acid, not only other fungi, but also bacteria of Bokryeong are not located. In addition, if the concentration of peracetic acid is too low or heat treatment is performed, other fungi will grow as well as bacterial seeds. It can be seen that the seed bacterium does not grow, and hydrogen peroxide is harmful to the culture of the Bokryeong spawn even though it is used at a low concentration.

Sawdust Sample 1 Sawdust Sample 2 Sawdust Sample 3 Sawdust Sample 4 Sawdust Sample 5 Degree of survival One X X 3 X Sawdust Sample 6 Sawdust Sample 7 Sawdust Sample 8 Sawdust Sample 9 Sawdust Sample 10 Degree of survival X One 5 X 3

<Example 4> Confirming the nematode present in the sawdust sample

As a result of detecting pine nematodes using Baerman Funnel for sawdust samples 1, 7, and 8, pine nematodes were not detected in sawdust samples 1 and 7, but re- nematodes were detected in sawdust samples 8. Through this, it can be seen that pine wilt disease can be killed when heat treatment is performed after chemical treatment using 1% peracetic acid solution of sawdust obtained from dead pine tree nematode disease where blue mold is growing.

<Example 5> Preparation of wood sample

1.Preparing a tree with a diameter of 15 cm and a length of 20 cm by cutting the pine tree nematode disease growing blue mold, immersing the tree in a 1% peracetic acid solution for 30 seconds and taking it out and using an oven set to 50°C. After drying for 250 minutes, the dried wood is packaged in a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of puffer fish equipped with a ventilation filter) and treated with high pressure steam at 121°C for 2 hours using an autoclave. By doing so, a sample 1 of the wood was obtained.

2. Cut a pine tree nematode disease tree growing blue mold, prepare a tree having a diameter of 15 cm and a length of 20 cm, immerse the tree in a 1% aqueous peracetic acid solution for 30 seconds, take it out and pack it in a heat-resistant polypropylene bag ( The sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter) Using autoclave, it was treated with high-pressure steam at 121°C for 2 hours to obtain a lumber sample 2.

3. Cut the dead tree of pine wilt disease growing on green mold, prepare solid wood having a diameter of 15 cm and a length of 20 cm, and pack the wood with a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter) ) Heat treatment at 121℃ for 2 hours with high pressure steam using autoclave, take out the wood from the bag, immerse it in 1% peracetic acid solution for 30 seconds, take it out and use it for 50 minutes using the oven set at 50℃ It was dried and re-packaged with a heat-resistant polypropylene bag (the sealed part was sealed with a cap for cultivation of puffer fish equipped with a ventilation filter) to obtain a woody sample 3.

4. Except for using a 0.5% peracetic acid aqueous solution in place of the 1% peracetic acid aqueous solution, other conditions were the same as those in Example 2, to obtain a lumber sample 4.

5. Other conditions were the same as those in Example 2 1, except that a 2% aqueous peracetic acid solution was used instead of a 1% aqueous peracetic acid solution, to obtain a title mark 5.

6. Prepare a log having a diameter of 15cm and a length of 20cm by cutting the pine tree nematode tree growing blue mold, and immersing the log in 80% methanol aqueous solution for 30 seconds, then taking it out and using the oven set at 50°C. 300 Dry sufficiently for a minute and pack the dried wood in a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of cultivated puffer fish equipped with a ventilation filter) and treated with high pressure steam at 121°C for 2 hours using an autoclave. Thus, a sample 6 of the wood was obtained.

7. Cut a normal pine tree (not contaminated with pine tree nematode and not contaminated with blue mold) to prepare a tree having a diameter of 15 cm and a length of 20 cm, and packing the tree with a heat-resistant polypropylene bag (the sealing part is a ventilation filter. Sealed with a cap for cultivation of the installed bokyeong) The autoclave was used for 2 hours at 121°C with high-pressure steam to obtain a lumber sample 7.

8. Cut a pine tree nematode disease tree growing blue mold, prepare a tree having a diameter of 15 cm and a length of 20 cm, and wrap the tree with a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter) ) The sample of wood was 8.

9. Prepare a log having a diameter of 15cm and a length of 20cm by cutting the pine tree nematode disease growing blue mold, and immersing the log in a 1% peracetic acid solution for 30 seconds and taking it out and using an oven set at 50℃. It was dried for 250 minutes and packaged with a heat-resistant polypropylene bag (the sealing part was sealed with a cap for cultivation of bok-ryeong equipped with a ventilation filter) to obtain a wood sample 9.

10 Cut a dead tree of pine wilt disease growing on green mold, prepare a tree having a diameter of 15 cm and a length of 20 cm, and pack the tree with a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter). The autoclave was treated with high pressure steam at 121°C for 2 hours to obtain a woody sample 10.

<Example 6> Confirmation of contamination of wood samples

1. For each wood sample obtained in Example 5, a hole (25 cm ² ) was formed using a drill to collect sawdust to a depth of 10 cm from the top surface, and then 10 ml of each sawdust and 30 ml of sterile water were added to a 50 ml conical tube. After putting vortex, 200 μl of the supernatant was smeared on potato agar medium (PDA), 7 days later, photographed and shown in FIGS. 4 to 6 (in the case of FIG. 4, using sawdust obtained from the lumber sample 1, in the case of FIG. 5) The use of sawdust obtained from, the use of sawdust obtained from wood sample 10 in the case of Figure 6), the type of colony (contamination) growing, the number of colonies, and the area of contamination are recorded in Table 3.

2. Referring to FIGS. 4 to 6 and Table 3, when the chemical treatment and heat treatment with 1% peracetic acid solution were performed on the trees obtained from the pine tree nematode disease growing trees with blue mold, the contamination did not occur, whereas the chemical treatment B. When only one of the heat treatments is performed or the concentration of the aqueous peracetic acid solution is low, it can be confirmed that contamination occurred.

Colony type Colony Count Contaminated area (%) Timber Sample 1 0 0 0 Timber Sample 2 0 0 0 Woodwork Sample 3 0 0 0 Timber Sample 4 One 5 10 Subject Sample 5 0 0 0 Timber Sample 6 0 0 0 Timber Sample 7 0 0 0 Timber Sample 8 One 50 90 Timber Sample 9 One 17 45 Timber Sample 10 2 13 30

<Example 7> Bokryeong culture using the woody sample

1. Sawdust sample (using sawdust sample 1 obtained in Example 1 1) 2 cm 3 of heat-treated rice per 10 cm 3, mixed with 0.03 g of Ca, placed in a container, inoculated with 0.07 g of liquid bokjae spawn, inoculated for 2 weeks at 26℃ After incubation, 5 g of sawdust cultured bacteria obtained after incubation was inoculated into each woody sample and cultured for 7 days at 26° C., and then photographed and shown in FIG. 7 (in the case of FIG. 7, woody sample 1 was used). It was confirmed and shown in Table 4. It was evaluated as X if nothing grew on the wood sample, and was evaluated as 1 to 5 according to the ratio of the fungal seed germ and other fungi growing on the wood sample, evaluated as 1 if only the germ germ grew, and 2 if the germ germ grew more than other fungi. , It was evaluated as 3 if the fungus was different from the other fungi, 4 if the fungus was less than the other fungus, and 5 if the fungus was only grown.

2. Referring to Table 4, when heat treatment is performed after chemical treatment using 1% peracetic acid solution for the raw material obtained from the dead tree of the pine wilt disease where the blue mold grows, the Bokryeong seed germ is well attached to the tree (also through FIG. 7) It can be seen that the seed germ adhered well to the tree), and if it does not undergo a drying process after chemical treatment or uses a high concentration of peracetic acid, it can be seen that the seed fungus as well as other fungi cannot be found in the tree. In addition, if the concentration of peracetic acid is too low or heat treatment is performed, other fungi, as well as cultivars of bok-ryeong, will grow on the lumber. It can be confirmed that the seed germ does not adhere to the head, and when chemical treatment is performed with methanol, the seed germ does not work well on the head.

Sawdust Sample 1 Sawdust Sample 2 Sawdust Sample 3 Sawdust Sample 4 Sawdust Sample 5 Degree of survival One X X 4 X Sawdust Sample 6 Sawdust Sample 7 Sawdust Sample 8 Sawdust Sample 9 Sawdust Sample 10 Degree of survival X One 5 X 3

<Example 8> Confirming the nematode present in the wood sample

As a result of detecting pine wilt using the Baerman Funnel for sawdust for the wood samples 1, 7 and 8 obtained in Example 6, pine wood wilt was not detected in the sawdust of wood samples 1 and 7, but the sawdust of wood sample 8 was re- nematode Was detected. Through this, it can be seen that the pine tree nematode can be killed when heat treatment is performed after chemical treatment using a 1% aqueous peracetic acid solution of the tree obtained from the dead tree of the pine wilt disease where blue mold is growing.

<Example 9> Cultivation of Bokryeong using pine tree nematode disease

Sawdust sample (use of sawdust sample 1 obtained in Example 1 1) 2 cm 3 of heat-treated rice per 10 cm 3, mixed with 0.03 g of Ca, placed in a container, inoculated with 0.07 g of liquid bokryeong spawn, incubated at 26° C. for 2 weeks After inoculation of 5 g of sawdust cultures obtained after the inoculation into each wood sample obtained in Example 5, cultured at 26° C. for 6 months, cultivation of boknyeong, photographed and shown in FIG. As can be seen in Figure 8, by using the pine tree nematode dead tree, sawdust badge, and make a tree, using this it can be confirmed that the well-cultivated bokyeong.

In the above, the applicant has described the preferred embodiments of the present invention, but these embodiments are only one embodiment for implementing the technical concept of the present invention and any modification or modification of the present invention as long as the technical concept of the present invention is implemented It should be construed as being within the scope.

Claims (10)

A method for producing sawdust medium for cultivation of Bokryeong spawn, characterized in that it comprises a sawdust harvesting step of obtaining sawdust by crushing the dead tree of pine wilt disease and a heat treatment step of applying heat to the sawdust. According to claim 1,
The manufacturing method of the sawdust medium for cultivation of Bokryeong seeds is a chemical treatment step of processing a dilute peracetic acid in sawdust obtained in the sawdust harvesting step, and after removing the sawdust from a peracetic acid diluent and drying it, remaining in the sawdust Further comprising a drying step of removing the acetic acid diluent,
In the heat treatment step, a method of manufacturing a sawdust medium for cultivation of bokryeong seeds, characterized in that heat is applied to the sawdust dried in the drying step. According to claim 2, The pine wilt disease dead wood
Method for producing sawdust medium for cultivation of Bokryeong spawn, characterized by being secondaryly infected by fungi. The method of claim 3, wherein the dilute peracetic acid
A method of manufacturing sawdust medium for cultivating bokryeong spawn, characterized in that a dilute peracetic acid is used in which peracetic acid is mixed with water to have a concentration of 0.8 to 1.2%. The method of claim 4, wherein the chemical treatment step
Method of producing a sawdust medium for cultivating bokryeong spawn, characterized in that the sawdust is immersed in dilute peracetic acid for 25 to 35 seconds. The method of claim 3, wherein the heat treatment step
Method of manufacturing a sawdust medium for cultivation of Bokryeong seed bacteria, characterized in that it is performed by applying high pressure steam to dried sawdust. The method of claim 3, wherein the heat treatment step
A method of manufacturing sawdust medium for cultivation of Bokryeong spawn, characterized in that high-pressure steam is added to the dried sawdust and heat-treated at 115 to 130°C for 70 to 120 minutes, so that the water content of sawdust is 50 to 70% after heat treatment. After inoculating the sawdust medium with sawdust medium for 2 to 3 weeks at a temperature of 24 to 28°C, a seed culture step of forming sawdust cultures, and chemical treatment of the pine tree wilt disease dead tree, followed by heat treatment to inoculate the sawdust cultures And a cultivation step of forming a lumber for the cultivation, and a cultivation step of inoculating sawdust cultivation on the lumber and then incubating to produce bokyeong.
The sawdust badge is a cultivation method of Bokryeong, characterized in that the sawdust badge manufactured by the method of any one of claims 3 to 7. The method of claim 8,
The tree formation step includes a chemical treatment step of treating a peracetic acid diluent on a tree obtained from a pine tree nematode disease, and a drying step of drying the chemically treated tree in the chemical treatment step to remove the peracetic acid diluent remaining in the tree. , Including the heat treatment step of heat treatment by applying heat to the wood dried in the drying step,
The pine wilt disease dead wood is a secondary infection method characterized in that the secondary infection by fungi. The method of claim 9,
In the chemical treatment step, a peracetic acid dilution solution is prepared by mixing peracetic acid with water to have a concentration of 0.8 to 1.2%, and the wood is immersed in the peracetic acid dilution solution for 25 to 35 seconds, or the peracetic acid dilution solution is 25 It is done by spraying for 35 seconds,
The heat treatment step is a high-pressure steam is applied to the dried wood by heat treatment at 115 to 130 ℃ for 100 to 150 minutes, characterized in that the cultivation method of bokryeong.

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