KR102111068B1 - Method for preparing log for cultivating poria cocos using tree died by pine wilt disease - Google Patents

Abstract

The present invention relates to a production method of xylem in Cudrania tricuspidata for cultivating poria cocos using a tree died by a pine wilt disease and, more specifically, to a production method of xylem in Cudrania tricuspidata for cultivating poria cocos using a tree died by a pine wilt disease, which improves economic efficiency by using a discarded tree died by a pine wilt disease and producing xylem in Cudrania tricuspidata for cultivating poria cocos, and improves the efficiency of poria cocos cultivation by performing heat treatment after chemical treatment using peracetic acid on the trees died by pine wilt disease.

Description

Method for preparing log for cultivating poria cocos using tree died by pine wilt disease}

The present invention relates to a method for manufacturing lumber for cultivation of bokyeong using pine tree nematode disease, more specifically, it is possible to manufacture lumber for cultivation of bokyeong using the old pine tree nematode disease that is discarded, thereby improving economic efficiency, and pine tree nematode disease It is about a method of manufacturing a lumber for cultivation of bokyeong using pine tree nematode disease that can improve the efficiency of cultivation of bokyeong by performing heat treatment after chemical treatment with peracetic acid.

Pine is an evergreen coniferous tree of the coniferous tree, the pine family. It grows all over Korea except for the northern highlands, and suffers a lot of damage from pine nematode disease. Pine nematode disease is a disease caused by pine nematodes, a nematode that parasites and eats trees, and the pine nematodes parasitize on the parasites such as the pine brine, and the northern beard sky, and are transferred to the trees through the vector. However, pine wilt disease has no treatment method so far, and the infection can be confirmed only with the naked eye, so many pine trees are killed by pine wilt disease. Therefore, various methods have been tried to use the pines killed by the pine wilt disease, and in general, the pine wilt disease is killed by using a heat treatment of the pine wilt disease as described in the following patent literature, and then the firewood is used.

<Patent Document>

Published Patent Publication No. 10-2009-0011397 (published on February 02, 2009) "How to recycle pine tree nematode disease and suspicious trees"

However, the conventional method of treating pine tree nematode disease has a problem in that the use of dead tree after heat treatment is limited by focusing only on killing the pine tree nematode through heat treatment. In particular, there is a problem that it is not possible to cultivate Bokryeong by using dead pine trees.

The present invention is to solve the problems of the prior art described above,

An object of the present invention is to provide a method of manufacturing a lumber for cultivation of bok-ryeong using pine lumber beetle ardwood, which can improve economic efficiency by manufacturing a lumber for cultivation of bok-ryeong using dead wood of pine wilt disease.

In addition, the present invention performs a heat treatment after chemical treatment with peracetic acid for pine tree nematode disease, pine tree nematode disease, which can effectively improve the efficiency of cultivation of cultivated worms by effectively removing molds that inhibit the growth of pine worms and worms The purpose of the present invention is to provide a method of manufacturing a lumber for cultivation of bokyeong using dead wood.

The present invention is implemented by an embodiment having the following configuration in order to achieve the above object.

According to an embodiment of the present invention, the method of manufacturing a lumber for cultivation of bokryeong according to the present invention includes a raw material obtaining step of obtaining a raw material by cutting a dead tree of pine wilt disease, and a heat treatment step of applying heat to the raw material to heat it. Is done.

According to another embodiment of the present invention, the method of manufacturing a lumber for cultivation of bokyeong according to the present invention includes a chemical treatment step of processing a dilute peracetic acid in the raw material obtained in the raw material obtaining step, and drying the raw material chemically treated in the chemical treatment step. It further comprises a drying step of removing the peracetic acid diluent remaining in the wood, and the heat treatment step is characterized in that heat is applied to the wood dried in the drying step.

According to another embodiment of the present invention, in the method of manufacturing lumber for cultivation of bokyeong according to the present invention, the pine tree nematode disease is characterized by being secondaryly infected by fungi.

According to another embodiment of the present invention, in the method of manufacturing a lumber for cultivation of bokryeong according to the present invention, the peracetic acid diluent is characterized in that a peracetic acid diluent is used by mixing peracetic acid with water to have a concentration of 0.8 to 1.2%. Is done.

According to another embodiment of the present invention, in the method of manufacturing the lumber for cultivation of bokyeong according to the present invention, the chemical treatment step immerses the wood in a dilute peracetic acid for 25 to 35 seconds, or dilutes the peracetic acid diluent in a raw material 25 to 35 It is characterized by being carried out by spraying for seconds.

According to another embodiment of the present invention, in the method of manufacturing the lumber for cultivation of bokyeong according to the present invention, the heat treatment step is characterized in that it is performed by applying high-pressure steam to the dried wood.

According to another embodiment of the present invention, in the method of manufacturing the lumber for cultivation of bokyeong according to the present invention, the heat treatment step is performed by applying high pressure steam to the dried wood and heat-treating it at 115 to 130° C. for 100 to 150 minutes. It is characterized by.

According to another embodiment of the present invention, the cultivation method of Bokryeong according to the present invention is a sawdust medium forming step of forming sawdust medium for cultivation of Bokryeong spawn by chemically treating the sawdust obtained from pine wilt disease, and then sawdust. A seed culture step of inoculating the medium with inoculated bokryeong seeds and forming a sawdust culture seed by incubation, and a incubation step of inoculating sawdust culture seeds in a tree and maintaining it at a temperature of 24 to 28°C for 5 to 7 months to produce bokryeong. Including, wherein the wood is characterized in that the wood produced by the manufacturing method of any one of claims 3 to 7.

According to another embodiment of the present invention, in the cultivation method of Bokryeong according to the present invention, the sawdust medium forming step is a chemical treatment step of processing a dilute peracetic acid solution on the sawdust obtained from pine tree wilt disease, and after the chemical treatment step And drying by removing sawdust from peracetic acid diluent and drying to remove the peracetic acid diluent remaining in the sawdust, and heat treatment by applying heat to sawdust dried in the drying step, wherein the pine wilt disease dead wood is moldy It is characterized by secondary infection by.

According to another embodiment of the present invention, in the cultivation method of Bokryeong in accordance with the present invention, the chemical treatment step prepares a peracetic acid dilution solution by mixing peracetic acid with water to have a concentration of 0.8 to 1.2%, and sawdust. It is performed by immersing in dilute peracetic acid for 25 to 35 seconds, and in the heat treatment step, high-pressure steam is added to the dried sawdust and heat-treated at 115 to 130°C for 70 to 120 minutes, so that the water content of sawdust after heat treatment is 50 to 70%. Characterized in that to have.

The present invention can obtain the following effects by the present embodiment.

The present invention has an effect that can improve the economic efficiency by producing a lumber for cultivation of bokyeong using dead wood pine wilt disease.

In addition, the present invention has an effect that can improve the efficiency of cultivation of bokyeong by effectively removing the fungus that inhibits the growth of pine elegans and boknyeong by performing heat treatment after chemical treatment with peracetic acid for pine tree nematode disease dead trees. .

1 to 3 is a picture for confirming the degree of contamination of the sawdust badge produced by the method of manufacturing a sawdust badge according to an embodiment of the present invention.
4 to 6 is a picture for confirming the degree of contamination of the wood produced by the wood manufacturing method according to another embodiment of the present invention.
Figure 7 is a photograph showing the appearance of the bokryeong spawn on the wood produced by the method of manufacturing wood according to another embodiment of the present invention.
Figure 8 is a photograph showing a cultivated bokyeong by the cultivation method of bokryong according to another embodiment of the present invention.

Hereinafter will be described in detail with reference to the accompanying drawings, a method of manufacturing a lumber for cultivation of bokryeong using pine tree nematode disease according to the present invention and a cultivation method of bokyeong using the lumber produced by the manufacturing method. Unless otherwise specified, all terms in this specification are the same as the general meaning of the term understood by a person skilled in the art to which the present invention belongs, and if there is a conflict with the meaning of the term used in the present specification, It follows the definition used in the specification. In addition, detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention are omitted. Throughout the specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated.

The method of manufacturing a lumber for cultivation of bokryeong using pine tree nematode disease according to an embodiment of the present invention is to heat the pine tree nematode disease tree after chemical treatment to form a tree for inoculating sawdust cultures, wood harvesting step, chemical treatment It includes a step, a drying step, and a heat treatment step. The pine tree nematode disease dead tree refers to a pine tree that has been pinched by a pine tree nematode disease, and is mostly secondaryly infected by fungi such as blue mold and Fusarium. The bokryeong spontaneously grows from dead pine trees. When the common pine tree nematode dead tree is used as simple heat treatment as in the prior art, it is possible to kill the pine wiltworm, but the cultivation of the bokryeong is impossible, and the present invention is secondary infection by fungi. It was completed to enable efficient cultivation of Bokryeong using the common pine wilt disease, and will be described in detail below.

The wood harvesting step is a step of obtaining a wood having a predetermined diameter and length by cutting pine dead wood.

The chemical treatment step may be performed by spraying a dilute peracetic acid on the raw wood obtained in the raw wood harvesting step, for example, by spraying the dilute peracetic acid on the raw wood or by immersing the raw material in a peracetic acid diluted solution for a certain period of time, peracetic acid Is mixed with water and a dilute peracetic acid having a concentration of 0.8 to 1.2% is used, and it is preferable to spray or immerse for 25 to 35 seconds.

The drying step is a step of drying the wood chemically treated in the chemical treatment step, for example, it may be performed by drying for 160 to 400 minutes using an oven set at 50°C. If the wood is not properly dried in the drying step, a dilute peracetic acid remains in the wood, thereby inhibiting the cultivation of the puffer fish.

The heat treatment step is a step of applying heat to the dried wood in the drying step, and it is preferable to heat the steam at 115 to 130° C. for 100 to 150 minutes by adding high-pressure steam to the dried wood. In the heat treatment step, heat treatment is performed using steam, so that the wood can have a water content capable of cultivating bok-ryeong. The heat treatment step may be performed using an apparatus such as an autoclave. It is possible to kill the pine wilt disease by immediately performing the heat treatment step on the wood obtained by cutting the pine wilt disease, but the present invention is impossible to cultivate bokyeong. After the heat treatment is performed to obtain a lumber capable of cultivation of bokyeong.

Another embodiment of the present invention relates to a lumber produced by a method of manufacturing lumber for cultivation of bokyeong using pine tree nematode disease.

Referring to Figures 1 to 8 of the cultivation method of bokryeong using lumber produced by a method of manufacturing a lumber for cultivation of bokyeong using pine tree nematode disease according to another embodiment of the present invention, the cultivation method of the bokyeong pine Sawdust medium forming step of forming sawdust medium for cultivation of bokreung seed germ by heat treatment after sawdust obtained from a dead tree disease, and seed cultivation step of culturing sawdust medium after inoculating bokreung seed germ to form sawdust culture , Bokryeong culture step of inoculating sawdust cultivation seed in the tree and then incubating to produce Bokryeong. As the above-mentioned tree, the tree manufactured by the method of manufacturing the tree for cultivation of bok-ryeong using the pine tree nematode dead tree described above is used, so a detailed description thereof will be omitted.

The sawdust medium forming step is a step of forming a sawdust medium for cultivation of cultivars in Bokryeong by chemically heat-treating sawdust obtained from pine tree nematode disease, sawdust harvesting step, chemical treatment step, drying step, heat treatment step, mixing step, etc. Includes.

The sawdust harvesting step is a step of obtaining sawdust by crushing the dead tree of pine wilt disease, and a conventional method of obtaining sawdust by crushing wood may be used.

The chemical treatment step is a step of processing a peracetic acid diluent in the sawdust obtained in the sawdust obtaining step (for example, dipping the sawdust in a dilute peracetic acid for a certain time), mixing peracetic acid with water to increase the concentration of 0.8 to 1.2%. A dilute peracetic acid solution is used and is preferably immersed for 25 to 35 seconds.

The drying step is a step of removing the sawdust from the peracetic acid dilution after the chemical treatment step and drying it, for example, it may be performed by drying for 160 to 200 minutes using an oven set at 50°C. If the sawdust is not properly dried in the drying step, a peracetic acid diluent remains in the sawdust, thereby inhibiting the culture of the Bokryeong spawn.

In the heat treatment step, heat is applied to the sawdust dried in the drying step, and high-pressure steam is added to the dried sawdust to heat treatment at 115 to 130° C. for 70 to 120 minutes, so that the water content of sawdust after heat treatment is 50 to 70. It is desirable to have %. In the heat treatment step, heat treatment is performed using steam, so that sawdust can have a water content capable of cultivation of Bokryeong spawn. The heat treatment step may be performed using an apparatus such as an autoclave.

The mixing step is a step of forming a sawdust medium by mixing rice bran, calcium, and the like with the sawdust heat-treated in the heat treatment step. It is possible to kill the pine wilt disease by performing the heat treatment step directly on the sawdust obtained by crushing the pine wilt disease, but the present invention is not capable of cultivation of the Bokyeong spp. After treatment and drying, heat treatment is performed to obtain a sawdust medium capable of cultivating Bokryeong spawn.

The seed culture step is a step of inoculating the sawdust medium obtained in the sawdust medium forming step and then incubating to form a sawdust culture seed, and then incubating the sawdust medium obtained from the dead pine tree and inoculating the seed germ seed germ. This can be used. For example, the sawdust medium can be placed in a container and liquid bokryeong seeds are added and maintained at a temperature of 24 to 28° C. for 2 to 3 weeks to form sawdust cultures.

The cultivation step is a step of inoculating sawdust cultivation obtained in the seed cultivation step and then incubating the cultivation to produce bokyeong, and a conventional method of inoculating and cultivating sawdust cultivation in the lumber obtained from dead pine trees is used. Can be. For example, the whole rice cultured seed can be inoculated into a tree and maintained at a temperature of 24 to 28° C. for 5 to 7 months to obtain bokryeong.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for explaining the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of sawdust samples

1. Sawdust is obtained by crushing the dead tree of pine wilt disease in which blue mold grows, and the sawdust is immersed in a 1% peracetic acid solution for 30 seconds, then taken out and dried for 180 minutes using an oven set at 50°C. The sawdust was treated with high pressure steam at 121° C. for 1 hour and 30 minutes using an autoclave to obtain sawdust sample 1.

2. Sawdust is obtained by crushing the dead tree of pine wilt disease in which blue mold is growing, and the sawdust is immersed in a 1% peracetic acid solution for 30 seconds, then taken out and used autoclave for 1 hour at 121°C with high pressure steam. After processing for 30 minutes, sawdust sample 2 was obtained.

3. Sawdust is obtained by crushing the dead tree of pine wilt disease in which blue mold is growing, and the sawdust is heat-treated at 121°C for 1 hour and 30 minutes with high-pressure steam using an autoclave, and the heat-treated sawdust is 1% and After immersing in acetic acid aqueous solution for 30 seconds, it was taken out and dried for 180 minutes using an oven set at 50° C. to obtain sawdust sample 3.

4. Sawdust sample 4 was obtained under the same conditions as in Example 1, except that a 0.5% peracetic acid aqueous solution was used instead of the 1% peracetic acid aqueous solution.

5. Sawdust sample 5 was obtained under the same conditions as in Example 1 except that a 2% aqueous peracetic acid solution was used instead of a 1% aqueous peracetic acid solution.

6. Sawdust sample 6 was obtained under the same conditions as in Example 1 except that a 0.3% hydrogen peroxide aqueous solution was used instead of the 1% peracetic acid aqueous solution.

7. Sawdust obtained by crushing a normal pine tree (not affected by pine tree nematode disease and not contaminated with green mold) was treated with autoclave for 1 hour and 30 minutes at 121°C with high pressure steam to obtain sawdust sample 7. .

8. Sawdust was obtained by crushing the dead wood of the pine wilt disease where green mold was growing, and the sawdust was used as sawdust sample 8.

9. Sawdust is obtained by crushing the dead tree of pine wilt disease in which blue mold is growing, and the sawdust is immersed in a 1% peracetic acid solution for 30 seconds, then taken out and dried for 180 minutes using an oven set at 50°C. Sample 9 was obtained.

10. Sawdust was obtained by crushing the dead tree of the pine wilt disease in which blue mold was growing, and the sawdust was treated with high pressure steam at 121°C for 1 hour and 30 minutes using an autoclave to obtain sawdust sample 10.

<Example 2> Confirmation of contamination of sawdust sample

1. Put 10 ml of each sawdust sample obtained in Example 1 and 30 ml of sterile water into a 50 ml conical tube, vortex and apply 200 µl of the supernatant on potato agar (PDA), after 7 days, photographed in FIGS. 1 to 3 Table 1 shows the type of growing colony (contamination), the number of colonies, and the area of contamination.(Table 1 is used for sawdust sample 1 in FIG. 1, sawdust sample 7 in FIG. 2, and sawdust sample 10 in FIG. 3) It is shown in.

2. Referring to FIGS. 1 to 3 and Table 1, while the chemical treatment and heat treatment using a 1% aqueous peracetic acid solution for sawdust obtained from the pine wilt diseased tree that grows blue mold, chemical contamination does not occur. B It can be confirmed that contamination occurs when only one of the heat treatments is performed or the concentration of the aqueous peracetic acid solution is low.

Colony type Colony Count Contaminated area (%) Sawdust Sample 1 0 0 0 Sawdust Sample 2 0 0 0 Sawdust Sample 3 0 0 0 Sawdust Sample 4 One 2 6 Sawdust Sample 5 0 0 0 Sawdust Sample 6 0 0 0 Sawdust Sample 7 0 0 0 Sawdust Sample 8 2 10 100 Sawdust Sample 9 One 5 30 Sawdust Sample 10 One 4 15

<Example 3> Culture of Bokryeong seeds using sawdust samples

1. After mixing 3 cm 3 of heat-treated rice bran, Ca 0.03 g per 10 cm 3 of each sawdust sample, put it in a container, inoculate 0.07 g of liquid bokryeong spawn, incubate at 26° C. for 7 days, and confirm the degree of spawning. It is shown in Table 2. If nothing grows on the sawdust sample, it is evaluated as X, and it is evaluated as 1 to 5 according to the ratio of the fungal seed germ and other fungi growing on the sawdust sample. It is evaluated as 1 if only the germ germ is grown, and 2 if the germ germ grows more than other fungi. , It was evaluated as 3 if the fungus was different from other fungi, 4 if the fungus was less than other fungus, and 5 if only the other fungus was grown.

2. Referring to Table 2, in the case of heat treatment after chemical treatment using 1% peracetic acid solution for sawdust obtained from the pine tree wilt diseased tree growing blue fungus, Bokryeong spawn grows well, but does not undergo drying after chemical treatment. It can be seen that when using a high concentration of peracetic acid, not only other fungi, but also bacteria of Bokryeong are not located. In addition, if the concentration of peracetic acid is too low or heat treatment is performed, other fungi will grow, as well as bok-ryeong seeds, and if the chemical treatment or chemical treatment is performed after heat treatment, the environment of sawdust medium will not adhere to the growth of bok-ryeong seeds, resulting in bok-ryeong. It can be seen that the seed germ does not grow, and hydrogen peroxide is harmful to the culture of Bokryeong seed germ even though it is used at a low concentration.

Sawdust Sample 1 Sawdust Sample 2 Sawdust Sample 3 Sawdust Sample 4 Sawdust Sample 5 Degree of survival One X X 3 X Sawdust Sample 6 Sawdust Sample 7 Sawdust Sample 8 Sawdust Sample 9 Sawdust Sample 10 Degree of survival X One 5 X 3

<Example 4> Confirm the nematode present in the sawdust sample

As a result of detecting pine nematodes using Baerman Funnel for sawdust samples 1, 7, and 8, pine nematodes were not detected in sawdust samples 1 and 7, but re- nematodes were detected in sawdust samples 8. Through this, it can be seen that the pine wilt disease can be killed when heat treatment is performed after chemical treatment with 1% peracetic acid solution of sawdust obtained from the dead tree of the pine wilt disease where blue mold is growing.

<Example 5> Preparation of wood sample

1.Preparing a tree with a diameter of 15cm and a length of 20cm by cutting the dead tree of pine wilt disease in which blue mold is growing, immersing the tree in 1% peracetic acid solution for 30 seconds and taking it out and using an oven set at 50℃ After drying for 250 minutes, the dried wood is packaged in a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of cultivated puffer fish equipped with a ventilation filter) and treated for 2 hours at 121°C with high pressure steam using an autoclave. By doing so, a sample 1 of the wood was obtained.

2. Cut the dead tree of pine wilt disease in which blue mold is growing, prepare a tree having a diameter of 15 cm and a length of 20 cm, immerse the tree in 1% peracetic acid solution for 30 seconds, take it out and pack it in a heat-resistant polypropylene bag ( The sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter.) Autoclave was used for 2 hours at 121° C. with high pressure steam to obtain a lumber sample 2.

3. Cut the dead tree of pine wilt disease growing on green mold, prepare solid wood having a diameter of 15cm and a length of 20cm, and wrap the wood with a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter) ) Heat treatment at 121℃ for 2 hours with high pressure steam using autoclave, take out the wood from the bag, immerse it in 1% peracetic acid solution for 30 seconds, take it out and use it for 50 minutes using the oven set at 50℃ The dried and heat-resistant polypropylene bag was re-packed (the sealed part was sealed with a cap for cultivation of puffer fish equipped with a ventilation filter) to obtain a woody sample 3.

4. Except for using a 0.5% peracetic acid aqueous solution in place of the 1% peracetic acid aqueous solution, other conditions were the same as those in Example 2, to obtain a lumber sample 4.

5. Other conditions were the same as those in Example 2 1, except that a 2% aqueous peracetic acid solution was used instead of a 1% aqueous peracetic acid solution, to obtain a title label 5.

6. Prepare a log having a diameter of 15cm and a length of 20cm by cutting the pine tree nematode tree growing blue mold, and immersing the log in 80% methanol aqueous solution for 30 seconds and taking it out and using the oven set at 50℃. Dry sufficiently for a minute, and pack the dried wood with a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of puffer fish equipped with a ventilation filter) and treated with high pressure steam at 121°C for 2 hours using an autoclave. Thus, a sample 6 of the wood was obtained.

7. Cut a normal pine tree (not contaminated with pine tree nematode and not contaminated with blue mold) to prepare a tree having a diameter of 15 cm and a length of 20 cm, and packaging the tree with a heat-resistant polypropylene bag (the sealing part is a ventilation filter. Sealed with a cap for cultivation of the installed bokyeong) The autoclave was used for 2 hours at 121°C with high-pressure steam to obtain a lumber sample 7.

8. Cut the dead tree of pine wilt disease in which blue mold is growing, prepare solid wood having a diameter of 15 cm and a length of 20 cm, and wrap the wood with a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter) ) The sample of wood was 8.

9. Prepare a log having a diameter of 15cm and a length of 20cm by cutting the dead tree of pine wilt disease in which green mold is growing, immerse the log in a 1% peracetic acid solution for 30 seconds, take it out and use an oven set at 50℃ It was dried for 250 minutes and packaged in a heat-resistant polypropylene bag (the sealing part was sealed with a cap for cultivation of bokyeong equipped with a ventilation filter) to obtain a woody sample 9.

10 Cut a dead tree of pine nematode disease, where blue mold is growing, prepare a tree with a diameter of 15 cm and a length of 20 cm, and pack the tree with a heat-resistant polypropylene bag (the sealing part is sealed with a cap for cultivation of bokyeong equipped with a ventilation filter). The autoclave was treated with high-pressure steam at 121°C for 2 hours to obtain a woody sample 10.

<Example 6> Confirmation of contamination of wood samples

1. For each wood sample obtained in Example 5, a hole (25 cm ² ) was formed using a drill to collect sawdust to a depth of 10 cm from the top surface, and then 10 ml of each sawdust and 30 ml of sterile water were added to a 50 ml conical tube. After putting vortex, 200 µl of the supernatant was applied to potato agar medium (PDA), 7 days later, photographed and shown in FIGS. 4 to 6 (in the case of FIG. 4, sawdust obtained from lumber sample 1, in FIG. 5, lumber sample 7 The use of sawdust obtained from, the use of sawdust obtained from wood sample 10 in the case of Figure 6), the type of colony (contamination) growing, the number of colonies, and the area of contamination are recorded in Table 3.

2. Referring to FIGS. 4 to 6 and Table 3, when the chemical treatment and heat treatment using a 1% aqueous peracetic acid solution are performed on the trees obtained from the pine tree nematode disease growing trees with blue fungi, the chemical treatment does not occur. B. When only one of the heat treatments is performed or the concentration of the aqueous peracetic acid solution is low, it can be confirmed that contamination occurred.

Colony type Colony Count Contaminated area (%) Timber Sample 1 0 0 0 Timber Sample 2 0 0 0 Woodwork Sample 3 0 0 0 Timber Sample 4 One 5 10 Subject Sample 5 0 0 0 Timber Sample 6 0 0 0 Timber Sample 7 0 0 0 Timber Sample 8 One 50 90 Timber Sample 9 One 17 45 Timber Sample 10 2 13 30

<Example 7> Bokryeong culture using a sample of wood

1. Sawdust sample (using sawdust sample 1 obtained in Example 1 1) 2 cm 3 of heat-treated rice per 10 cm 3, mixed with 0.03 g of Ca, placed in a container, inoculated with 0.07 g of liquid puffer seed strain, inoculated for 2 weeks at 26° C. After incubation, 5 g of sawdust cultured bacteria obtained after incubation was inoculated into each wood sample, and cultured for 7 days at 26° C., and then photographed and shown in FIG. 7 (in the case of FIG. 7, wood sample 1 was used). It was confirmed and shown in Table 4. It was evaluated as X if nothing grew on the wood sample, and was evaluated as 1 to 5 according to the ratio of the fungal germ and other fungi growing on the wood sample, and evaluated as 1 if only the germ germ grew, and 2 if the germ germ grew more than other fungi. , It was evaluated as 3 if the fungus was different from other fungi, 4 if the fungus was less than the other fungus, and 5 if the fungus was only grown.

2. Referring to Table 4, when heat treatment is performed after chemical treatment using 1% peracetic acid solution for the raw material obtained from the dead tree of the pine wilt disease where blue mold is growing, Bokryeong seed germ is well attached to the tree (also through FIG. 7) It can be seen that the seed bacteria adhered well to the tree), and after chemical treatment, the drying process is not carried out, or when high concentration of peracetic acid is used, it can be seen that the seed fungus as well as other fungi cannot be found in the tree. In addition, if the concentration of peracetic acid is too low or heat treatment is performed, other fungi, as well as cultivars in bok-ryeong, will grow in the lumber. It can be confirmed that the seed germ does not adhere to the head, and when chemically treated with methanol, the seed germ does not work well on the head.

Timber Sample 1 Timber Sample 2 Woodwork Sample 3 Timber Sample 4 Subject Sample 5 Degree of survival One X X 4 X Timber Sample 6 Timber Sample 7 Timber Sample 8 Timber Sample 9 Timber Sample 10 Degree of survival X One 5 X 3

<Example 8> Confirming the nematode present in the wood sample

As a result of detecting the pine wiltworm using Baerman Funnel for sawdust for the sawdust samples 1, 7 and 8 obtained in Example 6, the pine wiltworm was not detected in sawdust of sawdust samples 1 and 7, but the sawdust of sawdust sample 8 was re- nematode Was detected. Through this, it can be seen that the pine tree nematode can be killed when heat treatment is performed after chemical treatment with 1% peracetic acid solution of the raw material obtained from the dead tree of the pine wilt disease where blue mold is growing.

<Example 9> Cultivation of Bokryeong using pine tree nematode dead tree

Sawdust sample (using sawdust sample 1 obtained in Example 1 1) 2 cm 3 of heat-treated rice per 10 cm 3, mixed with 0.03 g of Ca, placed in a container, inoculated with 0.07 g of liquid puffer seed strain and incubated at 26° C. for 2 weeks After inoculation of 5 g of sawdust cultures obtained after inoculation into each wood sample obtained in Example 5, cultured at 26° C. for 6 months, cultivated boknyeong, and photographed and shown in FIG. 8. As can be seen in Figure 8, by using the pine tree nematode deadwood, sawdust badge, making a tree and using it can be confirmed that the well-cultivated bokyeong.

In the above, the applicant has described the preferred embodiments of the present invention, but these embodiments are only one embodiment for implementing the technical concept of the present invention and any modification or modification of the present invention as long as the technical concept of the present invention is implemented It should be construed as being within the scope.

Claims (10)

A wood harvesting step of obtaining a log by cutting dead pine trees of pine wilt disease, which is secondaryly infected by fungi, and a chemical treatment step of processing a peracetic acid diluted solution produced by mixing peracetic acid and water in the wood obtained in the wood harvesting step, and the chemical A drying step for drying the chemically treated wood in the treatment step to remove the peracetic acid diluent remaining in the wood, and a heat treatment step of applying heat to the dried wood in the drying step to heat treatment. Method of manufacturing. delete delete The method of claim 1, wherein the peracetic acid diluent
A method of manufacturing lumber for cultivation of bok-ryeong, characterized in that a dilute peracetic acid is used by mixing peracetic acid with water to have a concentration of 0.8 to 1.2%. The method of claim 1, wherein the chemical treatment step
Method for producing lumber for cultivation of bokyeong, characterized in that the raw wood is immersed in dilute peracetic acid for 25 to 35 seconds, or sprayed with dilute peracetic acid for 25 to 35 seconds. The method of claim 1, wherein the heat treatment step
Method of producing a lumber for cultivation of bokyeong, characterized in that it is performed by applying high-pressure steam to the dried wood. The method of claim 1, wherein the heat treatment step
Method of manufacturing a lumber for cultivation of bokyeong, characterized in that it is carried out by applying high-pressure steam to the dried wood at a temperature of 115 to 130° C. for 100 to 150 minutes. Sawdust medium forming step of forming sawdust medium for cultivation of cultivars in Bokryeong by chemically treating the sawdust obtained from pine tree nematode disease and heat treatment, and cultivating cultivation of sawdust cultivation after inoculating the sawdust medium and incubating it Wow, after inoculating sawdust cultivation spores on the lumber, and maintaining the temperature of 24 to 28 ℃ for 5 to 7 months, including a cultivation step of cultivating bokyeong,
The wood is a wood harvesting step to obtain the raw wood by cutting the dead tree of pine wilt disease, which is secondaryly infected by fungi, and a chemical treatment step of processing a diluted peracetic acid produced by mixing peracetic acid and water in the wood obtained in the wood harvesting step, , Drying by removing the peracetic acid diluent remaining in the raw material by drying the chemically treated raw material in the chemical treatment step, and being obtained through a heat treatment step of heat treatment by applying heat to the dried tree in the drying step. How to cultivate Bokryeong. The method of claim 8,
The sawdust medium forming step is a chemical treatment step of processing a dilute peracetic acid on sawdust obtained from pine tree nematode disease, and after removing the sawdust from the peracetic acid diluent, drying to remove the peracetic acid diluent remaining in the sawdust. A drying step and a cultivation method of bokryeong comprising a heat treatment step of applying heat to the sawdust dried in the drying step to heat treatment. The method of claim 9,
The chemical treatment step of the sawdust medium forming step is performed by preparing peracetic acid dilution solution by mixing peracetic acid with water to have a concentration of 0.8 to 1.2%, and dipping sawdust in the peracetic acid dilution solution for 25 to 35 seconds,
In the heat treatment step of the sawdust medium forming step, high-pressure steam is added to the dried sawdust and heat-treated at 115 to 130° C. for 70 to 120 minutes, so that the water content of sawdust after heat treatment is 50 to 70%. How to grow.

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