KR102220299B1 - Method for preparing sawdust media for cultivating poria cocos spawn using tree died by pine wilt disease - Google Patents

Abstract

The present invention relates to a method for manufacturing a sawdust medium for cultivating Bokryeong spawns using a pine tree nematode disease dead tree, and more particularly, a sawdust medium for cultivating Bokryeong spawns can be prepared using a discarded pine tree nematode disease dead tree, thereby improving economic efficiency. The production method of sawdust medium for cultivating Bokryeong spawns using pine tree nematode dead trees that can improve the efficiency of Bokryeong spawn culture by performing heat treatment after chemical treatment with peracetic acid on sawdust obtained from dead wood of pine nematode disease will be.

Description

Method for preparing sawdust media for cultivating poria cocos spawn using tree died by pine wilt disease}

The present invention relates to a method for manufacturing a sawdust medium for cultivating Bokryeong spawns using a pine tree nematode disease dead tree, and more particularly, a sawdust medium for cultivating Bokryeong spawns can be prepared using a discarded pine tree nematode disease dead tree, thereby improving economic efficiency. The production method of sawdust medium for cultivating Bokryeong spawns using pine tree nematode dead trees that can improve the efficiency of Bokryeong spawn culture by performing heat treatment after chemical treatment with peracetic acid on sawdust obtained from dead wood of pine nematode disease will be.

Pine is an evergreen coniferous arboreous tree of the coniferous coniferous pine family, and it grows all over Korea except in the northern highlands, and is suffering a lot of damage from pine tree nematode disease. Pine tree nematode disease is a disease caused by pine tree nematode, which is a nematode that is parasitic on pines and eats trees, and the pine tree nematode is parasitic to vector insects such as brush-bearing sky cattle and northern bearded sky cattle, and is transferred to trees through vector insects. However, there is no treatment method for pine tree nematode disease until now, and its infection can only be confirmed with the naked eye, so many pine trees are killed by pine tree nematode disease. Accordingly, various methods have been attempted to use pine trees killed by pine tree nematode disease. In general, the dead tree is used after the dead tree of pine tree nematode is killed by heat treatment as in the following patent documents.

<Patent Literature>

Unexamined Patent Publication No. 10-2009-0011397 (published on February 02, 2009) "Recycling method of trees damaged and suspicious of pine tree nematode disease"

However, the conventional method of treating the dead tree of pine tree nematode disease has a problem in that the use of the dead tree after heat treatment is limited by focusing only on killing the pine tree nematode through heat treatment. In particular, there is a problem that it is not possible to cultivate Bokryeong using the dead wood of pine tree nematode disease.

The present invention is to solve the problems of the prior art described above,

The present invention is to provide a method of manufacturing a sawdust medium for cultivating Bokryeong spores using a pine tree nematode cultivation that can improve economic efficiency by producing a sawdust medium for cultivating Bokryeong spores using discarded pine wood. There is this.

In addition, the present invention performs heat treatment after chemical treatment using peracetic acid on the sawdust obtained from the dead tree of pine nematode disease, effectively removing the fungus that inhibits the growth of pine nematode and bokryeong, thereby improving the efficiency of cultivation of Bokryeong spawns. It is about the manufacturing method of sawdust medium for cultivating Bokryeong spawns using the dead wood of pine nematode disease.

The present invention is implemented by an embodiment having the following configuration in order to achieve the above object.

According to an embodiment of the present invention, a method of manufacturing a sawdust medium for cultivating Bokryeong spawn according to the present invention includes a sawdust acquisition step of obtaining sawdust by pulverizing a pine tree nematode disease dead tree, and a heat treatment step of heat treatment by applying heat to the sawdust. Characterized in that.

According to another embodiment of the present invention, the method of manufacturing a sawdust medium for culturing Bokryeong spawn according to the present invention includes a chemical treatment step of treating the sawdust obtained in the sawdust acquisition step with a diluent peracetic acid, and the sawdust after the chemical treatment step. It further comprises a drying step of removing the peracetic acid dilution remaining in the sawdust by taking out from the acetic acid dilution solution and drying, and in the heat treatment step, heat treatment is performed by applying heat to the sawdust dried in the drying step.

According to another embodiment of the present invention, in the method of manufacturing a sawdust medium for cultivating Bokryeong spawn according to the present invention, the pine tree nematode disease dead tree is secondarily infected by fungi.

According to another embodiment of the present invention, in the method of manufacturing a sawdust medium for cultivating Bokryeong spawns according to the present invention, the peracetic acid dilution liquid is used to have a concentration of 0.8 to 1.2% by mixing peracetic acid in water It is characterized by being.

According to another embodiment of the present invention, in the method for manufacturing a sawdust medium for culturing Bokryeong spawn according to the present invention, the chemical treatment step is characterized in that the sawdust is immersed in a diluent of peracetic acid for 25 to 35 seconds.

According to another embodiment of the present invention, in the method of manufacturing a sawdust medium for culturing Bokryeong spawn according to the present invention, the heat treatment step is characterized in that it is performed by applying high-pressure steam to the dried sawdust.

According to another embodiment of the present invention, in the method of manufacturing a sawdust medium for cultivating Bokryeong spawn according to the present invention, the heat treatment step is performed by applying high-pressure steam to the dried sawdust and heat treatment at 115 to 130°C for 70 to 120 minutes. It is characterized in that the water content of the sawdust after heat treatment is 50 to 70%.

According to another embodiment of the present invention, the cultivation method of Bokryeong according to the present invention is a seed culture that forms a sawdust cultured seed by inoculating the Bokryeong seed in a sawdust medium and maintaining it at a temperature of 24 to 28°C for 2 to 3 weeks. Steps, including the step of forming a tree for inoculating sawdust cultured seed by heat treatment after chemical treatment of the dead tree of pine tree nematode disease, and a bokryeong culture step of inoculating and culturing the sawdust cultured seed tree to produce bokryeong. And, the sawdust medium is characterized in that the sawdust medium manufactured by the manufacturing method of any one of claims 3 to 7 is used.

According to another embodiment of the present invention, in the cultivation method of Bokryeong according to the present invention, in the step of forming a tree, a chemical treatment step of treating a dilute peracetic acid on a log obtained from a dead tree of pine nematode disease, and a chemical treatment step in the chemical treatment step A drying step of drying the treated logs to remove the peracetic acid dilution remaining on the logs, and a heat treatment step of heat-treating the logs dried in the drying step by applying heat, wherein the pine tree nematode dead wood is 2 It is characterized by secondary infection.

According to another embodiment of the present invention, in the cultivation method of bokryeong according to the present invention, in the step of forming a tree, the chemical treatment step is to prepare a diluted peracetic acid solution having a concentration of 0.8 to 1.2% by mixing peracetic acid with water. And, the log is immersed in the peracetic acid diluent for 25 to 35 seconds, or the peracetic acid diluent is sprayed on the log for 25 to 35 seconds, and the heat treatment step is performed by applying high pressure steam to the dried log to 115 to 130 It characterized in that it is carried out by heat treatment for 100 to 150 minutes at ℃.

The present invention can obtain the following effects by the above embodiment.

The present invention can produce a sawdust medium for cultivating Bokryeong spawns using the discarded pine tree nematode dead wood, there is an effect that can improve the economy.

In addition, the present invention performs heat treatment after chemical treatment using peracetic acid on the sawdust obtained from the dead tree of pine nematode disease, effectively removing the fungus that inhibits the growth of pine nematode and bokryeong, thereby improving the efficiency of cultivation of Bokryeong spawns. There is an effect.

1 to 3 are photographs for confirming the degree of contamination of the sawdust medium manufactured by the method of manufacturing a sawdust medium according to an embodiment of the present invention.
4 to 6 are photographs for confirming the degree of contamination of the timber manufactured by the method of manufacturing a timber according to another embodiment of the present invention.
7 is a photograph showing a state in which Bokryeong spawns are established on a tree manufactured by a method for manufacturing a tree according to another embodiment of the present invention.
Figure 8 is a photograph showing a bokryeong cultivated by the cultivation method of bokryeong according to another embodiment of the present invention.

Hereinafter, a method of manufacturing a sawdust medium for cultivating Bokryeong spawn using a pine tree nematode dead tree according to the present invention and a method of cultivating boryeong using the sawdust medium prepared by the above manufacturing method will be described in detail with reference to the accompanying drawings. Unless otherwise defined, all terms in this specification are the same as the general meaning of the terms understood by those of ordinary skill in the art to which the present invention belongs, and in case of conflict with the meaning of terms used herein, In accordance with the definitions used in the specification. In addition, detailed descriptions of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless specifically stated to the contrary.

The method of manufacturing a sawdust medium for cultivating Bokryeong spawns using a pine tree nematode dead tree according to an embodiment of the present invention is to form a sawdust medium for cultivating Bokryeong spawns by heat treatment after chemical treatment of sawdust obtained from a pine tree nematode dead tree. It includes an obtaining step, a chemical treatment step, a drying step, a heat treatment step, and a mixing step. The pine tree nematode disease dead tree refers to a pine tree that has been cut down by pine tree nematode disease, and is mostly secondary infection by fungi such as blue fungi and Fusarium genus. Bokryeong is native to dead pines, and if the above general pine tree nematode disease dead tree is used by simple heat treatment as in the prior art, it is possible to kill the pine tree nematode, but cultivation of bokryeong is impossible, and the present invention is secondary infection by fungi. It was completed in order to be able to efficiently cultivate Bokryeong using common pine tree nematode disease dead trees, which will be described in detail below.

The sawdust acquisition step is a step of obtaining sawdust by pulverizing the dead wood of pine tree nematode disease, and a conventional method of obtaining sawdust by crushing the wood may be used.

The chemical treatment step is a step of treating the sawdust obtained in the sawdust acquisition step with a diluted peracetic acid solution (e.g., immersing the sawdust in a diluent of peracetic acid for a certain period of time), and mixing peracetic acid with water to obtain a concentration of 0.8 to 1.2%. A diluent of peracetic acid is used and is preferably immersed for 25 to 35 seconds.

The drying step is a step of drying the sawdust after removing the sawdust from the peracetic acid diluent after the chemical treatment step. For example, it may be dried for 160 to 200 minutes using an oven set at 50°C. If the sawdust is not properly dried in the drying step, a diluent of peracetic acid remains in the sawdust, thereby inhibiting the cultivation of the Bokryeong spawn.

The heat treatment step is a step of applying heat to the sawdust dried in the drying step, and heat treatment at 115 to 130°C for 70 to 120 minutes by applying high pressure steam to the dried sawdust, so that the moisture content of the sawdust after the heat treatment is 50 to 70 It is desirable to have %. In the heat treatment step, since heat treatment is performed using steam, the sawdust may have a moisture content capable of cultivating Bokryeong spawns. The heat treatment step may be performed using an apparatus such as an autoclave.

The mixing step is a step of forming a sawdust medium by mixing rice bran, calcium, and the like with the sawdust heat-treated in the heat treatment step. The heat treatment step can be performed on the sawdust obtained by pulverizing the dead tree of pine tree nematode disease, but it is possible to kill the pine tree nematode, but cultivation of Bokryeong spawn is not possible.The present invention uses a peracetic acid solution for the sawdust obtained from the dead tree of pine tree nematode disease. After treatment and drying, heat treatment is performed to obtain a sawdust medium capable of cultivating Bokryeong spawn.

Another embodiment of the present invention relates to a sawdust medium prepared by a method of manufacturing a sawdust medium for cultivating Bokryeong spawns using a pine tree nematode dead wood.

Referring to Figs. 1 to 8, the cultivation method of Bokryeong using the sawdust medium prepared by the method of manufacturing a sawdust medium for cultivating Bokryeong spawns using a pine tree nematode dead tree according to another embodiment of the present invention will be described with reference to Figs. The cultivation method includes a seed cultivation step in which Bokryeong seed is inoculated on a sawdust medium and then cultured to form a sawdust cultivated seed, and a cultivation step of forming a seed tree for inoculating the sawdust cultivated seed by heat treatment after chemical treatment of the dead tree of pine tree nematode disease. , And a bokryeong culture step of producing bokryeong by inoculating the sawdust cultured spawn on the tree and then culturing it. The sawdust medium is a sawdust medium prepared by the method of manufacturing a sawdust medium for cultivating Bokryeong spawns using the aforementioned pine tree nematode dead wood, so a detailed description will be omitted.

The seed cultivation step is a step of inoculating the sawdust medium with Bokryeong seed and culturing it to form a sawdust culture seed, and a conventional method of inoculating and culturing the sawdust medium obtained from a dead pine tree may be used. For example, the sawdust medium may be put into a container, and liquid bokryeong seeds may be added and maintained at a temperature of 24 to 28°C for 2 to 3 weeks to form sawdust cultured seeds.

The timber forming step is a step of forming a timber for inoculating the sawdust cultured spawn by chemical treatment of the dead tree of pine tree nematode disease and then heat treatment, and includes a timber acquisition step, a chemical treatment step, a drying step, a heat treatment step, and the like.

The log-receiving step is a step of cutting the dead wood of pine tree nematode disease to obtain a log having a predetermined diameter and length.

The chemical treatment step is a step of treating the log obtained in the log acquisition step with a dilute peracetic acid solution, for example, spraying a diluent of peracetic acid on the log or immersing the log in a diluent of peracetic acid for a certain period of time. A diluent of peracetic acid prepared by mixing with water to have a concentration of 0.8 to 1.2% is used, and spraying or immersion for 25 to 35 seconds is preferable.

The drying step is a step of drying the wood chemically treated in the chemical treatment step, for example, may be performed by drying for 160 to 400 minutes using an oven set at 50°C. If the log is not properly dried in the drying step, a diluent of peracetic acid remains in the log, thereby inhibiting the cultivation of the bokryeong.

The heat treatment step is a step of applying heat to the wood dried in the drying step, and heat treatment is performed at 115 to 130°C for 100 to 150 minutes by applying high pressure steam to the dried wood. In the heat treatment step, since the heat treatment is performed using steam, the wood may have a moisture content capable of cultivating Bokryeong. The heat treatment step may be performed using an apparatus such as an autoclave. Although the above heat treatment step can be performed on the log obtained by cutting the dead tree of pine tree nematode disease, the heat treatment step can be performed to kill the pine tree nematode, but cultivation of bokryeong is not possible.In the present invention, the log obtained from the dead tree of pine tree nematode disease is treated with peracetic acid and dried. After the heat treatment is performed to obtain a tree that can be grown bokryeong.

The bokryeong cultivation step is a step of inoculating and culturing the sawdust cultured spawn obtained in the seedling cultivation step to the stock tree obtained in the birch formation step to produce bokryeong. The method of can be used. For example, it is possible to obtain bokryeong by inoculating the whole rice cultured seed germs on a tree and maintaining a temperature of 24 to 28°C for 5 to 7 months.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for describing the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of sawdust sample

1. Sawdust was obtained by crushing the dead tree of pine tree nematode disease in which blue mold is growing, and the sawdust was immersed in 1% peracetic acid aqueous solution for 30 seconds, then taken out and dried for 180 minutes using an oven set at 50℃, and dried. The sawdust was treated with high pressure steam at 121° C. for 1 hour and 30 minutes using an autoclave to obtain a sawdust sample 1.

2. Sawdust was obtained by crushing the dead wood of pine nematode disease where green mold is growing, and the sawdust was immersed in 1% peracetic acid aqueous solution for 30 seconds, and then taken out using high-pressure steam at 121°C for 1 hour using an autoclave. Treated for 30 minutes to obtain a sawdust sample 2.

3. Sawdust was obtained by crushing the deadwood of pine nematode disease where green mold is growing, and the sawdust was heat-treated at 121°C for 1 hour and 30 minutes with high-pressure steam using an autoclave, and the heat-treated sawdust was 1% and After immersing in an aqueous acetic acid solution for 30 seconds, it was taken out and dried for 180 minutes using an oven set at 50° C. to obtain a sawdust sample 3.

4. A sawdust sample 4 was obtained under the same conditions as in Example 1 except that 0.5% aqueous peracetic acid was used instead of the aqueous 1% peracetic acid solution.

5. A sawdust sample 5 was obtained under the same conditions as in Example 1 except that a 2% aqueous peracetic acid solution was used instead of the 1% aqueous peracetic acid solution.

6. A sawdust sample 6 was obtained under the same conditions as in Example 1 except that a 0.3% aqueous hydrogen peroxide solution was used instead of the 1% aqueous peracetic acid solution.

7. Sawdust obtained by crushing a normal pine tree (not affected by pine tree nematode disease and not contaminated with blue mold) was treated with high-pressure steam at 121°C for 1 hour 30 minutes using an autoclave to obtain a sawdust sample 7. .

8. Sawdust was obtained by crushing the deadwood of pine tree nematode disease in which green mold was growing, and the sawdust was used as sawdust sample 8.

9. Sawdust was obtained by crushing the dead tree of pine nematode disease where green mold is growing, and the sawdust was immersed in 1% peracetic acid aqueous solution for 30 seconds and then taken out and dried for 180 minutes using an oven set at 50°C. Sample 9 was obtained.

10. Sawdust was obtained by crushing the dead wood of pine nematode disease growing green mold, and the sawdust was treated with high pressure steam at 121°C for 1 hour 30 minutes using an autoclave to obtain a sawdust sample 10.

<Example 2> Confirmation of contamination of sawdust samples

1. Put 10 ml of each sawdust sample obtained in Example 1 and 30 ml of sterilized water into a 50 ml Conical Tube, vortex 200µl of the supernatant, and smear 200µl of the supernatant on potato agar medium (PDA). (In the case of FIG. 1, sawdust sample 1 is used, in the case of FIG. 2, sawdust sample 7 is used, and in the case of FIG. 3, sawdust sample 10 is used), and the type of growing colony, number of colony, and contamination area are recorded in Table 1 Shown in.

2. Referring to Figs. 1 to 3 and Table 1, when chemical treatment and heat treatment using 1% peracetic acid aqueous solution are performed on sawdust obtained from the dead wood of pine nematode disease in which blue mold is growing, contamination does not occur, whereas chemical treatment B. When only one of the heat treatment is performed or the concentration of the aqueous peracetic acid solution is low, it can be confirmed that contamination has occurred.

Colony type Colony Count Pollution area (%) Sawdust sample 1 0 0 0 Sawdust sample 2 0 0 0 Sawdust sample 3 0 0 0 Sawdust sample 4 One 2 6 Sawdust sample 5 0 0 0 Sawdust Sample 6 0 0 0 Sawdust Sample 7 0 0 0 Sawdust Sample 8 2 10 100 Sawdust sample 9 One 5 30 Sawdust sample 10 One 4 15

<Example 3> Bokryeong seed culture using sawdust sample

1. After mixing 2 cm 3 of heat-treated rice bran and 0.03 g of Ca per 10 cm 3 of each sawdust sample, put it in a container, inoculate 0.07 g of liquid Bokryeong seed, and incubate at 26°C for 7 days, and then check the extent of seeding. It is shown in Table 2. If nothing grows in the sawdust sample, it was evaluated as X, and it was evaluated as 1 to 5 depending on the ratio of the Bokryeong spawn growing on the sawdust sample and other fungi. , 3 if the Bokryeong spawn was similar to other fungi, 4 if the Bokryeong spawn grew less than other fungi, and 5 if only other fungi were grown.

2. As shown in Table 2, when heat treatment is performed after chemical treatment using 1% peracetic acid aqueous solution for sawdust obtained from dead wood of pine tree nematode in which blue mold is growing, bokryeong spawns grow well, but do not undergo drying after chemical treatment. It can be seen that if a high concentration of peracetic acid is used, not only other fungi but also Bokryeong spores cannot be found. In addition, if the concentration of peracetic acid is too low or only heat treatment, not only the Bokryeong spawn but also other fungi will grow.If only chemical treatment or chemical treatment after heat treatment, the environment of the sawdust medium cannot be bonded to the growth of Bokryeong spawn. It can be seen that the spawn cannot grow, and hydrogen peroxide is harmful to the cultivation of Bokryeong spawn even though it was used at a low concentration.

Sawdust sample 1 Sawdust sample 2 Sawdust sample 3 Sawdust sample 4 Sawdust sample 5 Survival One X X 3 X Sawdust Sample 6 Sawdust Sample 7 Sawdust Sample 8 Sawdust sample 9 Sawdust sample 10 Survival X One 5 X 3

<Example 4> Confirmation of re-nematodes present in sawdust sample

Pine tree nematodes were not detected in sawdust samples 1, 7 and 8 using Baerman Funnel, but pine tree nematodes were not detected in sawdust samples 1 and 7, but ash nematodes were detected in sawdust sample 8. Through this, it can be seen that if the sawdust obtained from the dead tree of pine tree nematode disease growing blue mold is chemically treated with 1% peracetic acid aqueous solution and then heat treatment is performed, the pine tree nematode can be killed.

<Example 5> Preparation of wood sample

1. Prepare a log having a diameter of 15cm and a length of 20cm by cutting the dead tree of pine tree nematode in which blue fungus is growing. After immersing the log in 1% peracetic acid solution for 30 seconds, take it out and use an oven set at 50℃. After drying for 250 minutes, the dried wood is wrapped in a heat-resistant polypropylene bag (the mouth is sealed with a cap for cultivation of Bokryeong equipped with a ventilation filter) and treated with high-pressure steam at 121°C for 2 hours using an autoclave. Thus, a stock sample 1 was obtained.

2. Prepare a log having a diameter of 15 cm and a length of 20 cm by cutting the dead tree of pine tree nematode in which blue mold is growing, and immerse the log in 1% peracetic acid solution for 30 seconds, take it out, and wrap it in a heat-resistant polypropylene bag ( The mouth-sealed part was sealed with a cap for cultivation of Bokryeong equipped with a ventilation filter.) Treated with high-pressure steam at 121°C for 2 hours using an autoclave to obtain Jawood Sample 2.

3. Prepare a log with a diameter of 15cm and a length of 20cm by cutting the dead tree of pine tree nematode in which green fungus is growing, and wrap the log with a heat-resistant polypropylene bag (the mouthpiece is sealed with a cap for Bokryeong cultivation equipped with a ventilation filter. ) Heat treatment at 121°C for 2 hours with high-pressure steam using an autoclave, remove the log from the bag, immerse it in 1% peracetic acid aqueous solution for 30 seconds, and then take it out and use an oven set at 50°C for 250 minutes. After drying and packaging again with heat-resistant polypropylene bags (the mouthpiece portion was sealed with a cap for cultivation of Bokryeong equipped with a ventilation filter), Jawood Sample 3 was obtained.

4. Except for using 0.5% peracetic acid aqueous solution instead of 1% peracetic acid aqueous solution, other conditions were carried out in the same manner as in Example 2 1, to obtain a purple tree sample 4.

5. Except for using a 2% aqueous peracetic acid solution instead of a 1% aqueous peracetic acid solution, other conditions were carried out in the same manner as in Example 2 1 to obtain a target table 5.

6. Prepare a log having a diameter of 15cm and a length of 20cm by cutting the dead tree of pine tree nematode in which blue fungus is growing. After immersing the log in 80% methanol aqueous solution for 30 seconds, take it out and use an oven set at 50°C to 300 Dry enough for a minute and wrap the dried log in a heat-resistant polypropylene bag (sealed with a cap for Bokryeong cultivation equipped with a ventilation filter) and treated with high-pressure steam at 121°C for 2 hours using an autoclave. Thus, a stock sample 6 was obtained.

7. Prepare a solid wood having a diameter of 15 cm and a length of 20 cm by cutting a normal pine tree (not affected by pine tree nematode disease and not contaminated with blue mold), and wrap the log in a heat-resistant polypropylene bag. Sealed with the attached Bokryeong cultivation cap) Using an autoclave, it was treated with high-pressure steam at 121° C. for 2 hours to obtain Jawood Sample 7.

8. Prepare a log with a diameter of 15 cm and a length of 20 cm by cutting the dead tree of pine tree nematode in which green fungus is growing, and wrap the log in a heat-resistant polypropylene bag (the mouth part is sealed with a cap for Bokryeong cultivation equipped with a ventilation filter. ) It was set as the tree sample 8.

9. Prepare a log having a diameter of 15cm and a length of 20cm by cutting the dead tree of pine tree nematode in which blue fungus is growing. After immersing the log in 1% peracetic acid solution for 30 seconds, take it out and use an oven set at 50℃. After drying for 250 minutes, it was packaged in a heat-resistant polypropylene bag (the part of the mouth was sealed with a cap for cultivation of Bokryeong equipped with a ventilation filter) to obtain a jawood sample 9.

10 Prepare a log having a diameter of 15 cm and a length of 20 cm by cutting the dead tree of pine tree nematode in which green fungus is growing, and wrap the log in a heat-resistant polypropylene bag (the mouth part is sealed with a cap for Bokryeong cultivation equipped with a ventilation filter) Treatment with high-pressure steam at 121° C. for 2 hours using an autoclave to obtain a jawood sample 10.

<Example 6> Confirmation of contamination of wood sample

^{1. A hole (25 cm 2} ) was formed using a drill for each of the wood samples obtained in Example 5, and sawdust was collected from the top to a depth of 10 cm, and then 10 ml of each sawdust and 30 ml of sterilized water were added to a 50 ml Conical Tube. After putting vortex, 200 µl of the supernatant was smeared on potato agar medium (PDA), photographed after 7 days, and shown in Figs. 4 to 6 (in the case of Fig. 4, sawdust obtained from the wood sample 1 is used, in the case of Fig. The use of sawdust obtained from, in the case of FIG. 6, the use of sawdust obtained from the wood sample 10), and the type of colony (contamination) growing, the number of colony, and the contamination area were recorded and shown in Table 3.

2. Referring to Figs. 4 to 6 and Table 3, when chemical treatment and heat treatment using 1% peracetic acid aqueous solution are performed on the tree obtained from the dead tree of pine nematode disease in which blue mold is growing, contamination does not occur, whereas chemical treatment B. When only one of the heat treatment is performed or the concentration of the aqueous peracetic acid solution is low, it can be confirmed that contamination has occurred.

Colony type Colony Count Pollution area (%) Arbor sample 1 0 0 0 Arbor sample 2 0 0 0 Arbor sample 3 0 0 0 Arbor sample 4 One 5 10 Wood sample 5 0 0 0 Arbor sample 6 0 0 0 Arbor sample 7 0 0 0 Arbor sample 8 One 50 90 Arbor sample 9 One 17 45 Wood sample 10 2 13 30

<Example 7> Bokryeong culture using Jamok sample

1. Sawdust sample (use of sawdust sample 1 obtained in Example 1) 2 cm 3 of rice bran and 0.03 g of Ca were mixed per 10 cm 3 and placed in a container and inoculated with 0.07 g of bokryeong seed in liquid form for 2 weeks at 26°C. 5g of sawdust cultured seeds obtained after cultivation during cultivation was inoculated into each seed tree sample and incubated at 26°C for 7 days, and then photographed and shown in FIG. It is shown in Table 4 to confirm. If nothing grows in the Jawood sample, it was evaluated as X, and it was evaluated as 1 to 5 depending on the ratio of the Bokryeong seed bacteria growing on the Jawood sample and other fungi. , 3 if the Bokryeong spawn was similar to other fungi, 4 if the Bokryeong spawn grew less than other fungi, and 5 if only other fungi were grown.

2. Looking at Table 4, when heat treatment after chemical treatment using 1% peracetic acid aqueous solution is performed on the logs obtained from the dead tree of pine tree nematode in which blue mold is growing, Bokryeong spawns are well established on the tree (see also Bokryeong It can be seen that the seed germs are well established on the tree tree), and if the drying process is not carried out after chemical treatment, or when a high concentration of peracetic acid is used, not only other fungi but also the Bokryeong seed germs cannot be found in the tree tree. In addition, if the concentration of peracetic acid is too low or only heat treatment, not only the Bokryeong spawn but also other fungi will grow on the tree, and if only chemical treatment or chemical treatment after heat treatment, the environment of the tree cannot be bonded to the growth of the Bokryeong spawn. It can be seen that the seed germs do not grow on the tree trees, and when chemical treatment with methanol is performed, the Bokryeong seeds do not grow well on the tree trees.

Arbor sample 1 Arbor sample 2 Arbor sample 3 Arbor sample 4 Wood sample 5 Survival One X X 4 X Arbor sample 6 Arbor sample 7 Arbor sample 8 Arbor sample 9 Wood sample 10 Survival X One 5 X 3

<Example 8> Confirmation of re-nematodes present in a tree sample

Pine tree nematodes were not detected in the sawdust of Jawood samples 1, 7 and 8 obtained in Example 6 using Baerman Funnel, but the sawdust of Jawood Sample 8 was not Was detected. Through this, it can be seen that when the solid wood obtained from the dead tree of pine tree nematode in which blue mold is growing is subjected to chemical treatment using 1% peracetic acid aqueous solution and then heat treatment is performed, the pine tree nematode can be killed.

<Example 9> Cultivation of Bokryeong using pine tree nematode disease dead tree

Sawdust sample (using the sawdust sample 1 obtained in Example 1) 2 cm 3 of heat-treated rice bran and 0.03 g of Ca were mixed per 10 cm 3 and placed in a container and inoculated with 0.07 g of Bokryeong seed in liquid form and incubated at 26°C for 2 weeks After inoculation of 5 g of the sawdust cultured seed bacteria obtained in Example 5, and inoculating each seedling sample obtained in Example 5 and cultivating Bokryeong for 6 months at 26° C., photographed and shown in FIG. 8. As can be seen in FIG. 8, it can be seen that even if a sawdust medium and a birch are made using the deadwood of the pine tree nematode, and using the same, bokryeong is well cultivated.

In the above, the applicant has described the preferred embodiments of the present invention, but such embodiments are only one embodiment that implements the technical idea of the present invention, and any change or modification of the present invention as long as the technical idea of the present invention is implemented. It should be construed as falling within the scope.

Claims (10)

A chemical treatment step of crushing a pine tree nematode disease dead tree secondary infected by fungi to obtain sawdust; a chemical treatment step of treating a dilute solution of peracetic acid produced by mixing peracetic acid and water with the sawdust obtained in the sawdust obtaining step; and the chemical A drying step of drying the sawdust treated chemically in the treatment step to remove the diluted peracetic acid remaining in the sawdust, and a heat treatment step of heat treatment by applying heat to the sawdust dried in the drying step. Manufacturing method of sawdust medium. delete delete The method of claim 1, wherein the peracetic acid diluent is
A method of manufacturing a sawdust medium for cultivating Bokryeong spawns, characterized in that a diluent of peracetic acid is used to have a concentration of 0.8 to 1.2% by mixing peracetic acid with water. The method of claim 1, wherein the chemical treatment step
Method for producing a sawdust medium for culturing Bokryeong spawns, characterized in that the sawdust is immersed in a diluted solution of peracetic acid for 25 to 35 seconds. The method of claim 1, wherein the heat treatment step
Method for producing a sawdust medium for cultivating Bokryeong spawns, characterized in that the high-pressure steam is applied to the dried sawdust. The method of claim 1, wherein the heat treatment step
A method of manufacturing a sawdust medium for culturing Bokryeong spawns, characterized in that high-pressure steam is added to the dried sawdust and heat-treated at 115 to 130°C for 70 to 120 minutes, so that the water content of the sawdust after the heat treatment is 50 to 70%. After inoculating Bokryeong spawn in sawdust medium, the seed culture step is maintained at a temperature of 24 to 28°C for 2 to 3 weeks to form sawdust cultured spawns, and sawdust cultured spawns are inoculated by heat treatment after chemical treatment of the dead tree of pine tree nematode disease. And a bokryeong cultivation step of forming bokryeong by culturing after inoculating sawdust cultivated spawn on the bokryeong, and
The sawdust medium is a chemical treatment step of pulverizing pine tree nematode disease dead trees secondary infected by fungi to obtain sawdust, and a dilute solution of peracetic acid produced by mixing peracetic acid and water with the sawdust obtained in the sawdust obtaining step And, a drying step of drying the sawdust chemically treated in the chemical treatment step to remove the peracetic acid diluent remaining in the sawdust, and a heat treatment step of heat treatment by applying heat to the sawdust dried in the drying step. How to cultivate Bokryeong. The method of claim 8,
The timber forming step includes a chemical treatment step of treating a diluent of peracetic acid on the log obtained from the dead wood of pine nematode disease, and a drying step of removing the diluent of peracetic acid remaining in the log by drying the chemically treated log in the chemical treatment step. And a heat treatment step of heat treatment by applying heat to the log dried in the drying step. The method of claim 9,
In the chemical treatment step, peracetic acid is mixed with water to prepare a peracetic acid dilution to have a concentration of 0.8 to 1.2%, and the log is immersed in the peracetic acid dilution for 25 to 35 seconds, or the peracetic acid dilution is added to the log for 25 seconds. By spraying for 35 seconds,
The heat treatment step is a cultivation method of bokryeong characterized in that it is carried out by applying high-pressure steam to the dried logs and heat treatment at 115 to 130 ℃ for 100 to 150 minutes.

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