KR20110068627A - Bag film for cultivating fruit tree having a degradable quality and insecticidable quality and manufacturing method thereof - Google Patents

Abstract

PURPOSE: A bag film for cultivating fruits with disinfection and antibacterial activity is provided to prevent abuse of an insecticide. CONSTITUTION: A bag film for cultivating fruits with disinfection and antibacterial activity comprises: a step of mixing 85 ml of distilled water and 15g of polyvinyl alcohol(PVA) and stirring at 80-85°C for 2-3 hours to prepare a PVA solution; a step of mixing 5-20 weight% of polyethylene glycol and stirring at 80-85°C for 30-90 minutes; a step of mixing 1-20 weight% of glyoxal and stirring at 80-85°C for 15-45 minutes; and a step of pouring a mixture on a flat board and drying at 50-70°C for 10-14 hours.

Description

BAG FILM FOR CULTIVATING FRUIT TREE HAVING A DEGRADABLE QUALITY AND INSECTICIDABLE QUALITY AND MANUFACTURING METHOD THEREOF}

The present invention relates to a fruit film encapsulation film having a degradable and antimicrobial insecticide and a method for manufacturing the same, and more particularly, to provide a fruit film encapsulation film having excellent degradability and antimicrobial insecticidal properties to prevent misuse of the pesticide during the fruit growing In addition, the present invention relates to a fruit film encapsulation film having a degradable and antimicrobial insecticide, and a method of manufacturing the same, which can solve a manpower shortage caused by spraying insecticides.

Generally, in fruit farms, plastic bags or paper bags are put on the fruits to protect the fruits from algae, pests, etc. and the wires are fixed on the top of the bags, or pins are inserted to fix the bags. It prevents pests and makes it possible to grow good quality fruits.

However, no matter how the bag is put, there is no preventive measures or treatments for the control of the germs that are infected by the infected germs or new rainwater before the bag filling operation.

After encapsulation, even if spraying pesticides, the control is not only low, but also can not be treated by the drug, because it is provided as a spawning place of various pests, the secondary damage is serious enough to be serious.

For this reason, in the case of fruits, the number and spreading amount of chemical pesticides sprayed in order to reduce the damage caused by various moths and powdery grains have been a problem, and the situation of causing environmental pollution because the waste paper bags are not easily disassembled.

The present invention was devised in view of the above-described circumstances, and an object of the present invention is to mix the high ginseng extract having antibacterial and insecticidal properties in the production of a fruit film encapsulation film, to prevent misuse of the pesticide during the cultivation of fruit trees, and to prevent farming of pesticides. To solve the labor shortage.

The present invention for achieving the above object, the neutralization degree 1700, saponification degree 98-99.5%, the average molecular weight of 50,000 g of polyvinyl alcohol (PVA: poly vinyl alcohol) mixture of 85ml of distilled water, the polyvinyl alcohol 15g 5 to 20% by weight of polyethylene glycol (Polyethylene Glycol), 1 to 20% by weight of glyoxal (Glyoxal) compared to 15g of the polyvinyl alcohol, prepared by mixing 10-30% by weight of high ginseng extract compared to 15g of the polyvinyl alcohol It is characterized by.

As described above, according to the present invention, by mixing the high ginseng extract having antimicrobial insecticidal properties in the manufacture of the fruit film encapsulation film is prevented from misuse of the pesticides in the cultivation of fruit trees, it is possible to supply more quality fruit water to consumers as well as excellent degradability It prevents environmental pollution and has the effect of solving the labor shortage in rural areas due to the spraying of pesticides.

Hereinafter, with reference to the accompanying drawings will be described in detail the encapsulation film for fruit growing having degradability and antimicrobial sterilization of the present invention.

Degradable and antimicrobial bactericidal fruit film encapsulation film according to the present invention is a mixture of 85 ml of distilled water to 15 g of polyvinyl alcohol (PVA) having a neutralization degree of 1700, saponification degree of 98 to 99.5%, an average molecular weight of 50,000, 5-20% by weight of polyethylene glycol (polyethylene glycol) compared to 15g polyvinyl alcohol, 1-20% by weight of glyoxal (Glyoxal) compared to 15g of the polyvinyl alcohol, 10-30% by weight of high ginseng extract compared to 15g of the polyvinyl alcohol It is prepared by mixing.

Here, PVA is a polymer produced by polymerization of vinyl acetate and saponification of polyvinylacetate obtained therefrom. PVA is an environmentally friendly polymer that is completely decomposed into water and carbon dioxide, and has biodegradability and biocompatibility. In addition, PVA has high strength and modulus of elasticity among general-purpose synthetic films, and thus, it can be applied to industrial materials and various applications.

In addition, the PVA polymer material varies the physical strength of the film by the difference in degree of polymerization and degree of saponification. The physical properties of the film can be confirmed by measuring the physical strength of the PVA film, such as tensile strength, elongation rate Max Force by the degree of polymerization and saponification. In addition, as a factor influencing the strength of the film, the type and the drying temperature of the crosslinking agent used in the film production are affected. In order to observe the mechanical properties of the degradable polymer film, first, several specimens according to the Korean Industrial Standard (KS. M3503) were fabricated using a specimen maker, and the thickness of the specimen was first measured. In this case, the thickness means a thickness when a decomposable film prepared between two parallel discs is applied and a constant pressure is applied. Using a thickness measuring instrument, the thickness of the specimen was measured five times according to the location, the minimum value was taken, and the scale was checked to a dimension of 0.001 mm. At this time, the minimum value is taken because the tensile strength and the elongation rate are cut at the portion where the thickness indicates the minimum value. Using the tensile strength and elongation rate measuring instrument, the moving speed of the specimen folds was arithmetic averaged by measuring 10 specimens under a load of 500 mm / min and 50 kg.

Table 1 below shows the change in mechanical strength due to saponification degree and polymerization degree of PVA, showing the highest tensile strength in P-17A (complete inspection, polymerization degree 1700), but the elongation is the lowest.

Max stress
(N / mm2) Max strain
(%) Max force
(N) Thickness
(mm) P-05A
(Partial inspection, degree of polymerization 500) 20.24 15.08 10.94 0.027 P-17A
(Partial inspection, degree of polymerization 1700) 32.10 60.60 17.35 0.03 P-20A
(Partial inspection, degree of polymerization 2000) 38.64 41.94 20.08 0.03 F-17A
(Complete inspection, degree of polymerization 1700) 51.02 3.10 28.82 0.03

In addition, Table 2 below shows the change in the mechanical strength of the film according to the type and content of the crosslinking agent used in the production of PVA film, when using Glyoxal as a crosslinking agent was relatively high tensile strength of 83.48N / ㎜, such Glyoxal When 0.5 wt% of borax (Na 2 B 4 O 7 .10H 2 O) is added to the content, it can be seen that the tensile strength and the elongation increase.

Max stress
(N / mm2) Max strain
(%) Max force
(N) Thickness
(mm) Glutaric
dialdehyde 54.54 4.98 52.04 0.03 Maleic anhydride 67.84 4.16 52.04 0.03 Glyoxal 83.48 2.44 65.06 0.03 Glyoxal +
Borax (0.5 wt%) 86.96 3.40 73.06 0.03 PVA (F-17A)
15wt% -Glyoxal 2% 39.43 3.80 31.54 0.03 PVA (F-17A)
15wt% -Glyoxal 4% 59.59 3.22 50.06 0.03 PVA (F-17A)
15wt% -Glyoxal 6% 66.36 4.51 58.40 0.03 PVA (F-17A)
15wt% -Glyoxal 8% 70.48 3.5 78.94 0.03 PVA (F-17A)
15wt% -Glyoxal 10% 61.94 3.74 49.56 0.03

In addition, Table 3 below shows the tensile strength of the film by the drying temperature of the film produced can obtain the best results at a drying temperature of 60 ℃.

Max stress
(N / mm2) Max strain
(%) Max force
(N) Thickness
(mm) 40 ℃ 65.82 3.50 33.40 0.03 60 ℃ 69.26 6.92 33.72 0.03 80 ℃ 61.08 4.58 30.02 0.03 100 ℃ 58.02 3.62 27.66 0.03 120 DEG C 57.84 3.38 38.20 0.03

And, looking at the manufacturing step of the fruit film encapsulation film of the present invention,

First, 85 ml of distilled water was mixed with 15 g of polyvinyl alcohol (PVA: polyvinyl alcohol) having a degree of neutralization of 1700, 98 to 99.5%, and an average molecular weight of 50,000, and stirred at 80 to 85 ° C. for 2 to 3 hours to prepare a PVA aqueous solution. do.

Then, 5 to 20% by weight of polyethylene glycol (Polyethylene Glycol) is added to 15 g of the polyvinyl alcohol as a plastic material and stirred at 80 to 85 ° C. for 30 to 90 minutes.

Thereafter, glyoxal (Glyoxal) is mixed with 1 to 20% by weight compared to the polyvinyl alcohol 15g and stirred for 15 to 45 minutes at 80 ~ 85 ℃.

Thereafter, the mixture is mixed evenly to the surface of the glyoxal evenly poured on a flat plate, dried for 10 to 14 hours in a dryer at 50 ~ 70 ℃, separated from the plate to complete the film production for making a fruit tree bag do.

Here, 1 g of distilled water was mixed with 100 g of powdered ginseng, heated at 70 to 80 ° C. for 1 to 2 hours, filtered, and concentrated through a rotary vacuum evaporator (polyvinyl alcohol). 10 ~ 30% by weight of ginseng extract compared to 15g of PVA aqueous solution preparation step, or polyethylene glycol agitation next step, glyoxal agitation and then any one of the steps selected by mixing and stirring.

In addition, all the ginseng extracts are mixed to add a phosphoric acid (phosphoric acid) to the stirred solution to the bar that can be adjusted to pH 2-3.

And, the ginseng used in the present invention is washed with distilled water in natural ginseng collected in nature and then dried in a shade to use completely dried.

Here, the ginseng is a perennial herb belonging to the legume, also called the cane of the thief, and its height is 80 ~ 100cm, and the pale yellow flowers in the shape of butterflies are shot in the summer. Blooming at the end of stems and branches, forming a legume (莢果), the roots are medicinal, coming from the mountains or fields, distributed throughout the country.

In the present invention, in order to leach a bioactive substance from the ginseng, and extract useful components therefrom, a distillation extraction apparatus of Rotavapor R-205 (BUCHI, SWITZERLAND) and FM-909T (HANIL, KOREA) grinder were used. Looking at the extraction yield of each solvent to select a suitable solvent for extracting bioactive substances from the ginseng, it can be seen that the high solvent in methanol and water extracts as shown in Table 1 below.

(Unit%) Sample Methanol Ethyl Acetate Ethanol Water (80 ℃) Sophora flavescens
powder 6.6 4.5 6.0 17.4

It can be seen that the water extraction yield is the highest as 17.4%, it is preferable to use water as the extraction solvent.

Here, the solvent-specific extraction of the sample is to quantitate 100g of each sample, and repeatedly extract methanol, ethyl acetate and ethanol three times with 1L each, and distilled water is heated to 80g at 1 ℃ for 1 to 2 hours at 100g of sample and then filtered. Concentrate on a rotary vacuum evaporator and use it as sample.

 In addition, looking at the matrin components according to the extraction conditions of high ginseng extract of the present invention, the matrin content for each solvent is 8205.8 mg / L in butanol solvent, 5012.8 mg / L in distilled water, 380.1 mg / L in ethyl alcohol, methanol Case 29020.7 mg / L, no nucleic acid was detected. Considering the economics and the process, the yield of matrin for distilled water and methanol extraction was the highest.

In the meantime, the antibacterial test of Gosam extract will be described.

In the present invention, the strain used for the antibacterial test of the high ginseng extract is Escherichia coli (KCCM 11234), Staphylococcus aureus (KCCM 12214), Glomerella cingulata (KCCM 11219), Alternaria kikuchiana (KCCM 11260), Botryotinia fuckeliana (KCCM 32314), Pseudomonas syringae (KCCM 41336) was used from the Korean spawn association. The culture of the strain was shaken in luria-bertani medium (LB, pH7.0, 37 ℃) medium, trypicase soy agar (TSA, pH7.2 37 ℃), Nutrient agar (NA, pH 7.0, 30 ℃) for 24hr. , fungi was shaken for 72 hours in PDA medium (pH 7.0, 24 ℃). These compositions were used to add 15% of Agar when using a solid medium. Phosphate buffer was used as the diluent, and the composition of the medium used for the growth of the strain is shown in Tables 5 to 9 below.

Composition of phosphate buffer Components K2HPO4 6 g KH2PO4 2 g Distilled water 1.0L

Composition of LB medium Components Trypton 10g Yest extract 5 g NaCl 10g Agar 15 g Distilled water 1.0L

Composition of trypicase soy agar Components Pancreatic digest of casein 17 g Pancreatic digest of soybean meal 3.0 g NaCl 5.0 g K2HPO4 2.5 g Agar 15 g Distilled water 1.0L

Components of PDA medium Components Potato Dextrose Broth 24 g Agar 15 g Distilled water  1 L

Components of Nutrient medium Components Beef extract 3.0 g Eptone 5.0 g Agar 15 g Distilled water 1.0L

The antimicrobial activity was measured by a disk diffusion test, optical density test and colony counting test.

Looking at the disc diffusion test (Disc diffusion test), after culturing 1-2 days in the optimum growth conditions of each strain was inoculated and suspended inoculated so that 1 × 10 ⁶ cells per 1ml medium. After sufficiently drying the soft agar on which the cells were spread, the paper disc was settled by aseptic operation (Autoclave 121 ° C, 15 min), and 100 µl of each extract solution was injected thereon to obtain optimal growth temperature of the cells. After incubation for time, the activity was searched for the presence of a clean zone.

In addition, the fiber was prepared by electrospinning the polymer solution containing the high ginseng extract extract, and the paper disc method was applied to confirm the antibacterial activity of the electrospun fiber containing the high ginseng extract. The fiber produced by electrospinning was cut to a constant size to 6 mm by using a punching machine (punching machine) and then subjected to a disc diffusion test (disc diffusion test). After placing the 6mm diameter specimen on the medium plated with 100µl strain and incubating for 24 hours at the optimal growth temperature of the smeared cells, the presence of proliferative hypoglycemia was examined through a video microscope (camsoope, sometech Ine, Korea). The diameter of the proliferative hypotonic ring was measured.

In addition, the optical density test (Optical density test) is inoculated 1ml strain to 100ml Potato dexterous broth and Nutrient broth growth medium of each strain. Add to the inoculated broth in the form of a solution based on the weight of the electrospun fiber containing each extract incubated in the optimum growth conditions (36 ℃, 180rpm) in a shaker at the same time every 4 hours from the inoculation time It was measured at 640 nm with a UV-2401PC spectrophotometer (SHIMADZU. Japan). In addition, the broth containing the extract was used as a blank to prevent the effect of the photochemical turbidity measurement by the turbidity of the extract.

The photochemical turbidity measurement of the electrospun fibers containing the respective extracts was measured at intervals of 4 hours at optimum growth conditions in shaker incubators after inoculating 1 ml of strain into 100 ml of each broth and adding 0.5 g of electrospun fibers.

In addition, the antibacterial activity of each fiber against Staphylococcus S. aureus was measured by colony counting method. Sufficient growth of the S. aureus strain was diluted 10 ⁴ fold by dilution phosphate buffer by serial dilution method. In 1 ml of the diluted strain and 99 ml of phosphate buffer solution, 0.5 g of the fiber of each ginseng extract was added, and colony counting was performed after 24 hours.

And, in order to test the insecticidal effect on the extract of red ginseng, peach heartworm ( Carposina sasakii Walsingham ) larvae were tested and leaf disk method was used as a test for the toxicity of peach heartworm larvae. The insecticidal effect of the larvae was laid on a 10 × 10 cm pear leaf and placed in a cotton-washed chalet moistened with water, inoculated with 30 peach heartworm larvae, and settled for 30 minutes. Sprayed for 5 seconds and then dried. The treated chalets were stored at room temperature and examined for fertilization rates after 24 and 72 hours. Life and death discrimination was regarded as a dead individual that did not move the length of the body by contacting the larvae with the tip of the brush through an anatomical microscope.

 After extracting ginseng with ethyl acetate and distilled water, concentrated to 1/16 and diluting it to 50, 100, 200 and 400 times, the insecticidal effect of peach heartworm larvae was tested. As shown in Table 10 below, the hatching rate of 50% was suppressed in 50 times dilution of ethyl acetate and distilled water extract.

Ethylacetate Water Treated water Hatching water Incubation rate (%) Treated water Hatching water Incubation rate (%) 50 times 34 17 50.0 32 16 48.4 100 times 26 13 51.9 60 29 48.0 200 times 39 24 61.5 50 29 59.1 400 times 39 31 79.3 32 28 87.6 No treatment 32 29 89.6 32 29 89.6

In addition, the spawning water of the adult was 10-12 untreated in ethyl acetate extract as shown in Table 11 and Table 11 below, but was lower by 0.2 after 72 hours in the 50-fold dilution treatment, and 3.5 peach cored moths in distilled water extract. It was confirmed that the suppression of scattering water.

Ethylacetate Water 24 hours 48 hours 72 hours 24 hours 48 hours 72 hours 50 times 0.6 1.1 0.2 4.0 2.1 3.5 100 times 1.1 0.4 2.8 5.9 4.8 3.5 200 times 1.0 1.4 2.4 4.3 4.1 4.1 400 times 2.6 2.2 1.6 3.8 5.6 4.3 No treatment 10.4 10.6 12.0 10.4 10.6 12.0

In addition, the insecticidal rate of the larvae of the peach heart moth was 72. As shown in Fig. 4 and Table 12 below, ethyl acetate and distilled water extract showed 79.2, 72.2% of the insecticide, respectively.

Ethylacetate Water Density before treatment 24 hours 72 hours Density before treatment 24 hours 72 hours 50 times 30 42.2 79.2 30 45.6 72.2 100 times 30 40.0 70.0 30 38.9 60.0 200 times 30 36.7 52.2 30 31.1 52.2 400 times 30 32.2 50.0 30 27.8 51.1 No treatment 30 2.2 6.7 30 2.2 6.7

As the above, the high ginseng extract is mixed in the fruit film encapsulation film to have antibacterial and insecticidal properties in the fruit tree to prevent the misuse of the insecticide during the fruit growing, to solve the labor shortage in rural areas required for the spraying of the insecticide, and to be excellent in degradability. Pollution can be prevented.

As described above in detail with respect to the present invention, those skilled in the art to which the present invention pertains will be capable of various modifications without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the above-described embodiments, but should be determined not only by the patent registration scope described later but by those equivalent to the claims.

1 is a block diagram showing the manufacturing step of the fruit film encapsulation film having a degradable and antibacterial insecticide according to the present invention,

Figure 2 is a graph showing the hatching rate of peach heart moth eggs by the treatment of ginseng extract,

Figure 3 is a graph showing the number of eggs of adult female peach heart moth adult ginseng extract treatment,

Figure 4 is a graph showing the insecticidal rate for peach heartworm larvae of ginseng extract.

Claims (3)

15 g of polyvinyl alcohol (PVA: polyvinyl alcohol) having a neutralization degree of 1700, a saponification degree of 98 to 99.5%, and an average molecular weight of 50,000, a mixture of 85 ml of distilled water, and 5 to 20 wt% of polyethylene glycol compared to 15 g of the polyvinyl alcohol. Glycol), 1-20% by weight of glyoxal (Glyoxal) compared to 15g of the polyvinyl alcohol, 10-30% by weight of high ginseng extract compared to 15g of the polyvinyl alcohol, characterized in that the degradability and antimicrobial insecticide The fruit film encapsulation having. Mixing an aqueous solution of 85 ml of polyvinyl alcohol (PVA: polyvinyl alcohol) to 85 ml and stirring the solution at 80 to 85 ° C. for 2 to 3 hours to prepare an aqueous solution of PVA; Stirring step of mixing 30 to 90 minutes at 80 ~ 85 ℃ by mixing 5 to 20% by weight of polyethylene glycol (Polyethylene Glycol) used as a plastic material compared to 15 g of polyvinyl alcohol (PVA: polyvinyl alcohol) in the aqueous solution manufacturing step Wow; A glyoxal mixing step of mixing 1 to 20% by weight of glyoxal (Glyoxal) with respect to 15 g of polyvinyl alcohol (PVA: polyvinyl alcohol) in the aqueous solution manufacturing step and stirring at 80 to 85 ° C. for 15 to 45 minutes; After the mixture of the glyoxal mixing step is evenly poured on a flat plate, the decomposing and antimicrobial insecticide cultivation encapsulation film prepared through a drying step of drying for 10 to 14 hours in a dryer of 50 ~ 70 ℃ Way. The method of claim 2, 1 g of distilled water mixed with 100 g of powdered ginseng, heated at 70 to 80 ° C. for 1 to 2 hours, and then filtered and concentrated through a rotary vacuum evaporator; The high ginseng extract mixing step of mixing the concentrated ginseng extract 10 ~ 30% by weight compared to 15g of polyvinyl alcohol (PVA: polyvinyl alcohol) in the aqueous solution manufacturing step is the aqueous solution manufacturing step, stirring step, glyoxal mixing step Method of producing a fruit film encapsulation film having a degradable and antibacterial insecticide, characterized in that any one of the following steps.

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