KR101640906B1 - Control composition of plant disease and noxious insects using microorganism and natural materials and producing method thereof - Google Patents

Abstract

The present invention relates to a composition for controlling harmful pests of plant horticulture using natural products and microorganisms and a method for preparing the same, and is a composition for controlling harmful insects of plant horticulture, which comprises an extract of a plant of Sophora flavescens and a strain containing antimicrobial activity , So that the pests can be controlled.

Description

TECHNICAL FIELD The present invention relates to a composition for controlling harmful plants and plants using natural products and microorganisms and a method for producing the same,

The present invention relates to a composition for controlling harmful insect pests and a method for preparing the same, and more particularly, to a composition for controlling harmful plants and plants of horticulture using natural materials and microorganisms and a method for producing the same.

The present invention also relates to a method for controlling pests of institutionalized horticulture.

In general, chemical insecticides have been used mainly for the control of moths, but chemical insecticides cause acidification and pollution problems of the soil, toxicity to the drawing, and environmental problems due to residual pesticide components. Due to recent environmental awareness and consumers' preference for eco-friendly foods, interest in eco-friendly farm products that can replace chemical pesticides has been growing, and its use is increasing.

Examples of eco-friendly farm products that can replace chemical insecticides include the use of plant-derived natural insecticides and the use of biological control agents utilizing natural enemies. In the case of moth control with natural extracts, it is often the case for individual farmers who have not been tested objectively. In addition, some products have been commercialized by some manufacturers, but they still have a lot of problems to be solved in order to replace chemical insecticides due to limitations of the effect of control, difficulty of manufacturing method, limited circulation period, etc. .

As an eco-friendly farming material, it has been attempted to use a ginseng root extract which is a natural plant ingredient having an insecticidal effect. However, most of them have been used in combination with other plants known to have an insecticidal effect from the past, It was not possible to use it as a substitute for chemical insecticide or as a moth control agent for controlling moths.

Gosam (Sophora flavescens) is a perennial herb of soybean and is a plant mainly distributed in Korea, Japan, China, and Siberia. In one room, dried root is called gamseng, and it is tasted, and it is prescribed to indigestion, neuralgia, hepatitis, jaundice, hemorrhoids, etc. In private, it is used as fever and pain medicine by dulling stem or leaf.

It is green with 80 ~ 100cm but it is black when it is young. Its stem is straight and has 15 ~ 40 small leaves, long oval or long ovate, 2 ~ 4cm long, 7 ~ 15mm wide, with long petiole, Do.

In other words, dried roots in one room called gosam, taste and ginseng has the efficacy of indigestion, neuralgia, hepatitis, jaundice, hemorrhoids and prescribes, in private, stem or leaf is also used as an insecticide.

Examples of such techniques are described in documents 1 and 2 below.

For example, Patent Literature 1 discloses a method in which the roots of ginseng are placed in 90 to 99% ethanol in an amount of 5 to 15 times the volume, extracted at 40 to 60 hours in a dark state at room temperature, filtered through a filter paper to obtain an extract, Concentrating the extract at 30 to 43 째 C with a vacuum concentrator, and lyophilizing the concentrated extract to produce a ginseng root extract for moth control.

In Patent Document 2, the roots of the roots are collected, shredded and finely pulverized to finely pulverize the roots. Then, the roots are washed with water having a volume of 20 times the weight of the roots of the roots of the roots, hexane, Alcohol, or a mixed solvent thereof having a mixing ratio of about 1: 0.1 to 1:10, preferably ethanol for about 8 days at about 5 to 80 占 폚, preferably about 24 占 폚, The supernatant was recovered by vacuum filtration using an extraction method such as extraction or ultrasonic extraction, and then the above procedure was repeated 2 to 5 times, preferably twice. The supernatant was collected and concentrated under reduced pressure to obtain a crude extract Based on the total weight of the composition.

Korean Registered Patent No. 10-1153181 (registered on May 30, 2012) Korean Registered Patent No. 10-1077178 (registered on October 20, 2011)

However, in the conventional techniques as described above, there is a problem that mass production is difficult, insecticidal power is insufficient, and therefore, it is not actually used and applied only in a laboratory.

Disclosure of the Invention The object of the present invention is to solve the above-mentioned problems, and an object of the present invention is to provide a pesticide complex controlling composition using an extract of a ginseng plant and a strain showing antibacterial activity, .

Another object of the present invention is to provide a composition for controlling harmful insect pests and a method for producing the same, which can produce a large amount of a combined insect pest controlling agent.

It is another object of the present invention to provide a control method for spraying and controlling pests of the horticulture.

To achieve the above object, the present invention provides a composition for controlling harmful insects of horticultural crops, which comprises an extract of a plant of Sophora flavescens and a strain showing antimicrobial activity.

In the composition of the present invention, the strain is Streptomyces spp.

The composition of the present invention is characterized in that the composition ratio of the extract to the strain is 30 to 70:70 to 30% by volume.

In addition, the composition of the present invention is characterized in that the composition ratio of the extract and the strain is 40: 60 by volume.

In addition, the composition according to the present invention may further comprise an adjuvant, wherein the adjuvant is added in a volume ratio of 1 to 10% based on 100% of the combined preparation of the extract and the strain.

In the composition according to the present invention, the adjuvant is polyoxyethylene dodecyl monoether.

(A) culturing a strain exhibiting an antimicrobial activity, (b) cultivating a strain exhibiting antimicrobial activity, and (b) Preparing a plant extract of Sophora flavescens; (c) mixing the strain cultivated in step (a) with the extract of the ginseng plant prepared in step (b).

(D) mixing the adjuvant in the step (c), wherein the adjuvant is selected from the group consisting of polyoxyethylene dodecyl mono ether ).

In the method of the present invention, the mixing ratio of the extract to the strain is 40: 60 by volume, and the adjuvant is 1 to 10% by volume relative to 100% of the combined extract of the extract and the strain Is added.

In addition, in the method for producing a composition for controlling harmful insect pests according to the present invention, the extract of step (b) is mixed after the culture of the strain according to the step (a).

In order to accomplish the above object, the present invention also provides a method for controlling harmful insects in a horticultural field, wherein the composition for controlling harmful insect pests includes any one of moths, moths, aphids, .

In order to achieve the above-mentioned object, the present invention also provides a method for controlling harmful insects in horticulture, which is characterized in that the above-described composition for controlling harmful insect pests is a plant pathogenic insecticide.

In the controlling method according to the present invention, the controlling treatment is a spraying treatment.

As described above, according to the composition of the present invention, it is possible to provide a complex control agent for pest insecticides, which is a pest insecticide containing extracts of the insect plants and a strain exhibiting antimicrobial activity, Room ( Spodoptera litura ), cigarette smoking in Louis ( Bemisia tabaci ) Adults, Plodia interpunctella ) Adult, cherry tree ( Amphitetranychus viennensis ) can be effectively controlled.

Further, according to the composition of the present invention and the method for producing the same, phytophthora capsici Leonian KACC is also obtained an effect that can control the 40 180 (plague).

In addition, according to the pesticide composite control agent composition and the production method thereof according to the present invention, it is also possible to obtain an effect of mass production of a pest-controlling compound controlling agent.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photograph showing the state of a strain having excellent sterilizing power applied to the present invention,
FIG. 2 is a photograph showing the result of insecticidal test for each dose of the adjuvant to tobacco,
FIG. 3 is a view showing a process for preparing a composition of a plant horticultural pest insecticide complex using a natural product and a microorganism according to the present invention. FIG.

These and other objects and novel features of the present invention will become more apparent from the description of the present specification and the accompanying drawings.

Hereinafter, the configuration of the present invention will be described with reference to the drawings.

First, microbial screening with excellent sterilizing power is described.

In the present invention, a microorganism having excellent sterilizing power was selected and identified from one strain of Micro Innovation Inc., one strain belonging to Keimyung University Bacteriology Laboratory, and three microbial strains isolated from soil samples. Soil samples were diluted to 10 ² to 10 ³ and then plated on oat meal plate (oat meal 30 g, agar 15 g / L) in an amount of 100 μl each, dried, and cultured at 28 ° C for 7 days. The strains grown on the plate medium were sterilized by pure separation.

The microorganisms having excellent bactericidal activity were identified from various strains, and then, as shown in Table 1 below, Bacillus The strains exhibiting antimicrobial activity of 18 mm in subtilis PCI 219 were selected. The microorganisms having excellent germicidal activity were identified as actinomycetes by microscopic examination as shown in A and B of FIG. 1, and named as SG strain ( Streptomyces spp.).

Fig. 1 (A) is a photograph showing a state of being inoculated on a YM medium and cultured at 28 캜 for 7 days, and Fig. 1 (B) is a microscope photograph showing a 400-fold magnification of Fig.

Table 1 shows the results of the transformation of E. coli KCTC 1682 and Bacillus subtilis It exhibits antimicrobial activity against PCI 219.

The extracts of Sophora flavescens plants to be applied to the present invention are obtained by purchasing roots of ginseng root, washing and drying and powdering the roots, and then spraying the seeds with hexane, chloroform, ethyl acetate extraction with cold extraction, reflux cooling extraction or ultrasonic extraction for 1 to 10 hours at room temperature with C1 to C4 lower alcohols such as ethyl acetate, methanol, ethanol or the like or a mixed solvent thereof at room temperature Method, and the supernatant is recovered by vacuum filtration. The above procedure is repeated three times, and the supernatant is collected and concentrated under reduced pressure to obtain the extract. However, the extract of the alpine plant applied to the present invention is not limited to the above-mentioned method, but may be carried out by applying a known method.

In the present invention, when the microorganism is cultured in the presence of the extract, the antifungal activity is increased but the microorganism has an effect on the growth of the strain. Therefore, it is expected that the microorganism will be a problem in commercialization. I thought about how to do it.

Bacillus a 18mm antimicrobial activity of the subtilis PCI 219 shown strain (hereinafter, 'SG strains (Streptomyces spp.)'quot;) and to a Sophora flavescens extract were mixed in a composition ratio as shown in Table 2 eseo 35 ± 2 ℃ after the composite formulation The packed cell volume, inhibition rate against plant pathogens, and control rate against pests were determined through aging changes at intervals of 4 weeks for 12 weeks.

Table 2 shows the combined formulation ratio.

The aging change was carried out according to the 'criteria and method of aging change test (common to microbial pesticides and biochemical pesticides)' of the 'Examination methods for registration of biological pesticides and registration application documents' notified by Rural Development Administration.

The pesticide complex control composition composition according to the present invention treats any one of pests, moths, aphids, mites and powdery moths.

In addition, the composition according to the present invention is effective for controlling a disease caused by a plant pathogen.

Phytophthora capsici Leonian KACC 40180 (Plasmodium vivax), which has an excellent antifungal activity against plant pathogens, was used. The samples were collected and diluted 1,000 times with 20 μl of paper disc (= 6mm, Whatman Co.) , Dried, and placed on a plate culture medium containing a black stain. After incubation at 30 ° C for 7 days, the diameter of the inhibitory zone produced was determined.

The insecticidal rate of insect pests was maintained at 25 ± 2 ℃, light intensity 16L: 8D, and relative humidity of 50 ~ 60% in the 3rd instar larvae of Spodoptera litura susceptible strain distributed in Andong, Korea. . The insecticidal effect was obtained by inoculating caleb leaves with 8 cm diameter petridish (φ90 mm) with filter paper soaked in distilled water, inoculating 10 larvae of Tobacco spp. For 10 seconds each with a hand sprayer and dried in a hood for 2 hours. Each treatment was carried out in 3 replicates per treatment and untreated was sprayed with distilled water. After treatment, the mortality rate was measured 48 hours after storage in the breeding room.

As shown in Table 3, when the selected ginseng extract was mixed with five kinds of composition ratio, the compression cell volume of the microorganism was observed to be about 15% for 12 weeks, I could. The inhibition rate of plant pathogens and the insecticidal rate of insect pests were the highest at 60% of the SG strain of sample 4 and 40% of the ginseng extract, indicating that the combination of sample 4 had the best effect. In addition, the efficacy of the combination product was 10% in 12 weeks, indicating that the duration of efficacy of the combination product was also good.

Table 3 shows the compression cell volume (P.C.V.), the inhibition rate of plant pathogens, and the insecticidal rate according to aging time.

In Table 3, the rate of inhibition of plant pathogens and the insect pest insecticide ratio in the composition of 60% of the SG strain and 40% of the ginseng extract in the sample 4 are about 60 to 70% The purpose of this study was to select an adjuvant for increasing the efficacy in the formulation of combination control agents.

Among the nonionic surfactants used in general agricultural applications, the physical properties of four surfactants with high dispersibility and proven stability were examined (Table 4). Table 4 shows the adjuvants used in the test.

Four types of surfactants are listed in the Inert Ingredients List of the pesticide products prescribed by the US Environmental Protection Agency (EPA) specified in the Agriculture, Forestry and Livestock Food Ordinance 'Enforcement of the Act on the Promotion of Environmentally Friendly Farming and Fisheries and Management of Organic Foods' 3 or 4, only those substances that can be used as adjuvants in the production of organic agricultural materials were selected.

Next, the physical properties of the adjuvants for enhancing the efficacy of the mixed preparations were examined.

① Diffusion test

Each of the adjuvants was diluted 2,000 times on a flat table and dropped 20 쨉 l three times. Four minutes later, the diffusion area was examined.

② Adhesion test

Each of the supplements was diluted 2,000 times, and the pepper leaves were immersed in a beaker for 5 seconds and placed on a test table.

③ Drying time test

Each of the supplements was diluted 2,000 times, the pepper leaves were immersed in a beaker for 5 seconds, placed on a test table, and the time when the test agent was dried at 90% or more was measured.

④ Pore force test

Each supernatant was diluted 2,000 times, and the cells were shaken 20 times by adding a quantitative flask, and the shape of the supernatant after 10 minutes was compared.

The test results were as follows.

① Diffusion test

As a result of the diffusion test on the test table with each adjuvant, the best adjuvant was polyoxyethylene dodecyl mono ether, followed by Polyoxyethylene sorbitan monooleate, Polyoxyethylene (1,1,3, 3-tetramethylbutyl) phenyl ether, and sodium bis (2-ethylhexyl) sulfosuccinate.

Table 5 shows the degree of diffusion for each adjuvant.

② Adhesion test

Polyoxyethylene sorbitan monooleate, polyoxyethylene (1,1,3,3-tetramethylbutyl) phenyl ether, and sodium bis (1,1,3,3-tetramethylbutyl) phenyl ether were the most adherent agents. 2-ethylhexyl) sulfosuccinate.

Table 6 shows the degree of adhesion of each adjuvant.

③ Drying time test

Polyoxyethylene sorbitan monooleate (PLE) was the fastest adjuvant for pepper leaves. Polyoxyethylene dodecyl mono ether (BKS-1), Polyoxyethylene (1,1,3,3-tetramethylbutyl) phenyl ether, Sodium bis -ethylhexyl) sulfosuccinate.

Table 7 shows the drying time for each adjuvant.

④ Pore force test

As a result of the vesicidal test, the best adjuvant was polyoxyethylene dodecyl mono ether, followed by Polyoxyethylene sorbitan monooleate, Polyoxyethylene (1,1,3,3-tetramethylbutyl) phenyl ether and Sodium bis (2-ethylhexyl) sulfosuccinate .

Table 8 shows the vapors per supporter.

Next, the efficacy of the adjuvant to increase the efficacy of the combined preparation was investigated.

First, the inhibition rate against plant pathogenic bacteria was examined.

The physical properties of the adjuvant were evaluated as the best effect of polyoxyethylene dodecyl mono ether. The inhibition rate of plant pathogens was confirmed to be 1,000 times dilution by adding 1 to 10% by volume of 100%

As described above, the inhibition rate against phytopathogenic fungi is Phytophthora was conducted for capsici Leonian KACC 40180 (plague), the 20㎕ to 1,000-fold dilution of the sample was collected paper disc (= 6mm, Whatman Co. ) was added to the dry laid on a plate medium containing the test strains The diameter of the inhibition range generated after culturing at 30 ° C for 7 days was measured and determined.

As described above, the insecticidal rate of insects was 25 ± 2 ℃ in temperature, 16L: 8D in light condition and 50 ~ 60% in relative humidity in the 3rd instar larvae of Tobacco spp. The cage leaves were cut into 8 cm diameter and placed on filter paper, which was soaked in distilled water. Ten birds of tobacco budworm larvae were inoculated, and the compound preparation was inoculated for 10 seconds at 1,000 times concentration. hand sprayer and dried in a hood for 2 hours. Each treatment was carried out in 3 replicates per treatment and untreated was sprayed with distilled water. After treatment, the mortality rate was measured 48 hours after storage in the breeding room.

As shown in Table 9 below, when the polyoxyethylene dodecyl mono ether was added, the inhibition rate and pest insecticidal rate against the plant pathogenic bacteria were increased. The addition ratio was the most effective when added in a 10% volume ratio to 100% of the composite preparation sample 4, and the result was lower than that. Therefore, it was concluded that the addition of the adjuvant in a 10% volume ratio to 100% of the sample preparation 4 of the combination preparation would be ideal for optimal formulation.

Table 9 shows the rate of inhibition of plant pathogens and the insecticidal rate against pests by the input ratio of polyoxyethylene dodecyl mono ether.

Next, the efficacy of the functional group farewell to various insect pests of the selection aid was verified.

In case of insect pests, it is generally controlled by the action mechanism by contact or wet when it is controlled by the plant extract. Further experiments have been conducted on how the selected adjuvant has an effect on the action mechanism.

The tobacco is Louis ( Bemisia tabaci) was observed insecticidal rate after processing in a manner that the holster each formulation between parafilm in order to test the oral toxicity heupjeup them directly for adults. The amount of the used treatment formulation was 300 μL for each treatment group with a dilution factor of 1,000, and 20% sucrose diluted solution was commonly used for all treatments. Tobacco powder was used in the greenhouse of the Department of Agricultural Biology, Kyungpook National University.

As a result of observation for 48 hours after the treatment, the addition ratio of the auxiliaries generally used in the farms was 10%, the insecticidal rate was 100% at 20%, 93.33 ± 3.85% at 5% And 75.55 ± 5.87%, respectively. As a result of checking the insecticidal power by time, it was confirmed that the insecticidal rate was the best when the amount of the adjuvant was 10%.

Table 10 shows the oral toxicity of various concentrations of the adjuvant, and Fig. 2 is a photograph showing the results of the insecticidal test for each dose of the adjuvant to the tobacco.

Next, the insecticidal effect on the moth moth was carried out.

Plodia interpunctella) to the Indian Mealmoth feeding 1g to test the oral toxicity of the adult-fold dilution 1,000 mixed evenly to 1mL was added to each of the processing distinguished, then dried to feed for one day to measure the insecticidal rate and emergence rate.

Hwangryonggam moth was used in the greenhouse of Kyungpook National University.

As shown in Table 10, 10%> 20%> 5%> 0% of the insecticidal rates of the insecticides were higher in the order of 0% (Table 11).

As a result of observation for 30 days after the treatment, the feral rate was not alienated when the supplementary inputs were 5%, 10%, 20%, and 5% when no supplementary agent was added (Table 12)

Table 11 shows the insecticidal rate of each formulation against the moth moth, and Table 12 shows the rate of occurrence of each formulation relative to the moth moth.

Next, insecticidal effect on cherry mite was carried out.

Amphitetranychus viennensis ) were sprayed at a distance of 50cm with a dilution of 1,000 times at each leaf surface attached to the cherry tree mite. The mortality rate was monitored for 18 hours in Chare (φ90mm).

As a result of observation for 18 hours after the treatment, the dosage of supplementary adjuvant was 10% at 20%, 100% at 5%, and 43.3 ± 3.3 at 5% . From the above results, it was confirmed that the insecticide rate was the best and economical when the supplementary dose was 10%.

Table 13 shows the insecticidal rate of each formulation against cherry mite.

The optimal formulation of the combination preparation according to the invention was then determined.

Based on the conclusions from the above experiments, a composite preparation sample was prepared with the composition of Table 14 as the optimal mixing condition. The sample was changed at intervals of 2 days at 35 ± 2 ° C for 4 weeks at 0 ± 2 ° C for 12 weeks Physical activity, inhibition rate against plant pathogens and control rate against pests.

Table 14 shows the optimal formulation composition of the combination preparation.

The aging change was carried out according to the 'criteria and method of aging change test (common to microbial pesticides and biochemical pesticides)' of the 'Examination methods for registration of biological pesticides and registration application documents' notified by Rural Development Administration.

Changes in physical properties were judged by visual inspection at dilution. The inhibition rate against phytopathogenic fungi was Phytophthora was conducted for capsici Leonian KACC 40180 (plague), the 20㎕ to 1,000-fold dilution of the sample was collected paper disc (= 6mm, Whatman Co. ) was added to the dry laid on a plate medium containing the test strains The diameter of the inhibition range generated after culturing at 30 ° C for 7 days was measured and determined.

The insecticidal rate of insect pests was maintained at 25 ± 2 ℃ in temperature, 16L: 8D in light condition and 50 ~ 60% in relative humidity in the 3rd instar larvae of Tobacco spp. , And the insecticidal effect was obtained by inoculating 10 ml of tobacco budworm larvae and third instar larvae in a shale (φ90 mm) in which filter paper moistened with distilled water was cut to a diameter of 8 cm, and the compound preparation was sprayed for 10 seconds with a manual sprayer And dried in a hood for 2 hours. Each treatment was carried out in 3 replicates per treatment and untreated was sprayed with distilled water. After treatment, the mortality rate was measured 48 hours after storage in the breeding room.

As a result of examining physical properties, inhibition rate of plant pathogens and insecticidal rate against insect pests according to aging change of samples prepared with optimal combination composition of combination preparation, it was found that the physical properties and efficacy of the combination preparation were remarkably stable . In addition, it is shown that the stabilization for 12 weeks at 35 ± 2 ℃ on the aging change standard indicates that the efficacy is guaranteed for 1 year. Therefore, it is also possible to set the guarantee period of the prototype product to 1 year or more in the future.

Table 15 shows physical properties, plant pathogen inhibition rate, and pest control rate at 35 ± 2 ° C over time, and Table 16 shows physical properties, plant pathogen inhibition rate, and pest control rate at the time of 0 ± 2 ° C over time.

Next, prototype preparation and on-site evaluation of the combined preparation were carried out.

The prototype was manufactured through the manufacturing process of FIG. 3 with the composition composition of the final combination as shown in Table 15, and the indoor pot test for the blight of the red pepper and the tobacco cracking room was performed.

FIG. 3 is a view showing a process for producing a composition of a plant and horticultural pest insecticide complex using a natural product and a microorganism according to the present invention.

As shown in FIG. 3, pre-culture is performed with a seed medium and a production medium so that the pH is 7, followed by mainculture. The culture conditions were 30 ° C, 200 rpm, air volume 0.5 vvm, and cultured in a 300 L pilot plant for four days at 150 L working volume.

In this culturing process, the culture solution is stirred by a motor to increase the culturing speed. To this cultured medium, the extract of Bombyx mori L. plant was mixed with polyoxyethylene dodecyl mono ether, which is an adjuvant, and filtered to produce a final product, a complex formulation.

That is, in the present invention, as shown in FIG. 3, the microorganism was cultured alone, and then the plant extract, Gossam extract, was mixed.

Phytophthora capsici Leoniana KACC 40180 (Plague) pathogens were harvested for the SG strain and the mixed strains were mixed with the soil (80 ml on the soil: 20 ml, 10 ⁵ spores / ㎖) The leaf surface was treated with the diluted water at a concentration of 1,000 times per week for one to three times. Patients were observed at intervals of 7 days after the first week of treatment.

The control for insect pests was placed in an acrylic box for insect breeding (30 cm in length, 30 cm in height, 28.5 cm in height) with a diameter of 8 ㎝ and a height of 7 ㎝, planted with red pepper seedlings, Respectively. After 1 hour to allow larvae to settle completely on the leaves, they were sprayed at a distance of 50 cm at a concentration of 1,000 times. Forty-eight hours after the treatment, the presence or absence of death of the pest was determined by determining that the larvae were dead if they were unable to raise the larva by using a thin brush. One port was repeated with 3 iterations.

In order to determine whether the prototype product was weak or not, on the spot, we sprayed three times per week for the dilution factor of 1,000 times and the amount of dilution, which was the actual treatment concentration, and observed the weakness every 7 days after the treatment. The evaluation of the weakness was carried out according to the 'criteria and method of the weak test' of the 'Pesticide registration test standards and methods' notified by Rural Development Administration.

In the case of pepper blight, the pathogen alone treatment showed 96.7% incidence in the final investigation, but in the case of single treatment, 43.3% of the treated plants showed 56.7%. In the case of 3 treatments, the incidence rate was about 8.3%, showing more than 90% control (Table 17).

After 24 hours of treatment, 80.0% of control was observed in the tobacco budworm, and 86.7% of control was observed after 48 hours (Table 18).

Table 17 shows the control of the pepper lice inhibition by the number of treatments of the prototype, Table 18 shows the control value against the pests after the treatment of the prototype, and Table 19 shows the occurrence of the weakness after the treatment of the prototype.

Although the present invention has been described in detail with reference to the above embodiments, it is needless to say that the present invention is not limited to the above-described embodiments, and various modifications may be made without departing from the spirit of the present invention.

By using the composition of the present invention and the method for producing the same, it is possible to control the pests of the horticultural plants.

Claims (13)

delete delete delete delete delete delete A method for producing a composition for controlling harmful insects,
(a) culturing Streptomyces spp. as a strain exhibiting antimicrobial activity at 28 ° C for 7 days,
(b) preparing a plant extract of Sophora flavescens,
(c) mixing the extract of step (b) after culture of the strain according to step (a)
(d) mixing the adjuvant in step (c)
In step (b), the roots of the ginseng plant were washed, dried and powdered, and extracted with chloroform or ethyl acetate in a volume of 20 times the weight of the ginseng powder for 10 hours at room temperature To obtain a crude extract,
The adjuvant is polyoxyethylene dodecyl monoether,
Wherein the mixing ratio of the extract to the strain is 40: 60 by volume, and the adjuvant is added in an amount of 10% by volume relative to 100% of the combined extract of the extract and the strain. delete delete delete As a method for controlling pests of the horticulture in the facility,
Characterized in that the composition produced by the method for producing a pest-control compound composition according to claim 7 is a pest control composition, wherein any one of pests, aphids, mites, and powdery mushrooms is treated. As a method for controlling pests of the horticulture in the facility,
Characterized in that the composition produced by the method for producing a composition for controlling pest combinatorial disease as set forth in claim 7 is an antiseptic treatment for a disease caused by a plant pathogenic bacterium. 12. The method according to claim 11 or 12,
Wherein the controlling treatment is a spraying treatment.

Images