KR101153181B1 - A Method of Preparing the Insecticidal Extract of Sophorae Radix for Moth, the Agents comprising it and the Method of Controling Moth - Google Patents

Abstract

The present invention relates to a ginseng root extract showing a moth moisturizing activity and a moth using the extract, in the present invention, after extracting the ginseng root in a dark state at room temperature for 40 to 60 hours with ethanol to obtain the extract by filtration, it is a reduced pressure concentrator After concentrating at 30 ~ 43 ℃ to provide a method for producing a ginseng root extract to be lyophilized and a moth-proof composition using the obtained ginseng root extract and a moth control method using the same. Since the ginseng root extract according to the present invention alone exhibits excellent moth control activity of more than 80% to 90%, it can be used as an effective mothicide in place of chemical pesticides that have been used.

Red ginseng, moth medicine, Chinese cabbage moth, insecticide, oxymatrine, matrine

Description

A method of preparing the Insecticidal Extract of Sophorae Radix for Moth, the Agents comprising it and the Method of Controling Moth}

The present invention relates to a moth-preventing agent, and more particularly, to a moth-preventing agent and its extraction method using the natural extract from plants as an active ingredient.

In general, chemical insecticides have been used to remove pests such as Chinese cabbage moth, black moth, green chestnut moth, tobacco moth, leaf oyster fly. Chinese cabbage moth ( Plutella) xylostella ) is a pest belonging to the Lepidoptera house moth family, which mainly produces cruciferous crops such as cabbage, radish, and cabbage, and is also called parachute by some farms. Chinese cabbage moth is a global pest that is widely distributed from tropical regions of Southeast Asia such as Philippines and Thailand to high latitudes of northern Canada. In addition, the larvae dig into the epidermis from the back of the leaves of the host crop, leaving only the epidermis and cooling the leaf flesh.In particular, when a lot of crop seedlings occur, the larvae eat the young leaves and leave only the leaf veins, which leads to death. It gives a significant impediment to early growth. It was only in the mid-1980s that this pest began to be a problem in our country, and it began to occur in vegetable growing complexes in some suburbs. The main cause of the increase in the occurrence of Chinese cabbage moths is that the growing conditions of the cabbage, cabbage, radish, etc., which are the main vegetables of cruciferous plants, have been increasing recently, and the conditions of feeding have been improved as the planting of cruciferous vegetables through year-round cultivation through facility cultivation, such as plastic houses, As the cultivation of cruciferous vegetables has become simple and continuous, natural enemies have been reduced due to the concentrated spraying of drugs for pest control of vegetables. The effect is falling, causing more damage and damage. An adult insect is a small moth 6 mm in length and 12 to 16 mm in length. The wings are grayish black or grayish yellow and are pests that mustard crops such as cabbage and cabbage. Eggs are oval, flat, pearlescent, reticulated, pale yellow green. The larvae are cylindrical, slender in front and back, and the body is usually green, but there are many variations such as pale yellow, yellow red, and gray. Occurs several times a year, especially in May-June but rarely occurs in summer and again in autumn. The eggs are spawned one by one on the front and back side of the food grass, and the larvae eat only the leaves along the veins. As it grows, it is parasitic on the back of the leaves and eats, leaving only the outer skin, so if the damage is severe, the whole crop looks white.

Chemical pesticides have been used mainly for moth control, but chemical pesticides cause soil acidification and pollution, toxicity to human beings, and environmental problems due to pesticide residues. Recently, due to increasing awareness of environmental problems and consumers' preference for eco-friendly foods, there is a growing interest in eco-friendly agricultural materials that can replace chemical pesticides. Eco-friendly agricultural materials that can replace chemical insecticides include the use of natural pesticides extracted from plants and the use of biological control factors utilizing natural enemies. In the case of moth control using natural extracts, it is often the level of folk remedies for individual farmers whose effects have not been objectively verified. In addition, there are some products that have been commercialized by some manufacturers, but there are still many problems to be solved before they can be commercialized by replacing chemical insecticides due to limitations in the control effect, difficulty in manufacturing method, and limited shelf life. . In particular, in the case of a product having a poor control effect, it may cause a secondary problem caused by an increase in natural populations, and thus may not be a preferable control method.

Attempts have been made to use ginseng root extract as a natural plant ingredient with insecticidal effects, but most of them have been mixed with other plants known to have insecticidal effects in the past. It could not be used or used as a moth-preventing agent for controlling moths.

One aspect of the present invention is to provide a method for effectively extracting the active ingredient so that the ginseng root extract has a sufficient moth-proofing effect, and thus the extracted ginseng root extract.

Another aspect of the present invention is to provide an effective moth-proof composition and a method for controlling moths using the same as the active ingredient ginseng root extract according to the present invention.

Other objects and advantages of the present invention will be described below and will be better understood by practice of the present invention.

In the present invention, a method for obtaining a ginseng root extract exhibiting excellent moth-proofing activity and a ginseng root extract thus obtained are provided.

Method for producing a ginseng root extract having a moth-proofing activity of the present invention,

(A) step of obtaining the extract by filtering the ginseng root in 5 to 15 volume times of ethanol for 40 to 60 hours in a dark state at room temperature and then filtered with a filter paper;

(b) completely extracting the extract obtained above at 30-43 ° C. with a reduced pressure concentrator;

(c) lyophilizing the concentrated extract.

Ginseng is a perennial plant that grows on legumes, sunny meadows, plains, roadsides, sandy lands, and sunny ocher lands, and is native to Korea, Japan, China, and Siberia. Sophora Root is the total alkaloid content of 1.1% oxy-Mart Lin (Lin -N- _{Mart-oxide) C 15 H 24 N 2 O} , Mart Lin C ₁₅ H ₂₄ N ₂ O, parcel ranol C ₁₅ H ₂₄ N ₂ O, Anagiline C ₁₅ H ₂ 0N ₂ O, Methylcytazine C ₁₂ H ₁₆ N ₂ O, Baptifolin C ₁₅ H ₂ 0N ₂ O ₂ , Sopocarpine C ₁₅ H ₂₄ N ₂ O? H ₂ O, Allomatrine It contains components such as C ₁₅ H ₂₆ N ₂ , and also contains about 13% of sugar. Dried roots of ginseng have the taste and efficacy of ginseng, and have been used for the treatment of indigestion, neuralgia, hepatitis, jaundice, and hemorrhoids. Due to this efficacy, there are many examples of using ginseng as one of the plant insecticide components.

In order to obtain a plant extract having insecticidal activity from plants such as ginseng, alcohol extraction after hot water extraction, alcohol extraction while heating to a constant temperature, or heating and concentration after alcohol extraction have been used. However, the present inventors systematically studied the ginseng, and concluded that it is necessary to extract alcohol for a long time at room temperature without cancer in order to obtain an extract with high insecticidal activity from the ginseng. To this end, in the present invention, goose root is placed in 5 to 15 times the volume of ethanol and extracted 40 to 60 hours in a dark state at room temperature, followed by filtration with filter paper to separate only the extract. Ethanol is preferably at least 90% ethanol, more preferably at least 95% ethanol. The extraction time which soaks the root of ginseng root in ethanol becomes like this. Preferably it is 48 hours or more. The ginseng root used for extraction is preferably dried to 1 to 2 mm after drying to 5% or less of water content. The extract thus obtained is concentrated completely at 30 ~ 43 ℃ using a vacuum concentrator, wherein the temperature does not exceed 43 ℃. At higher temperatures, the pesticidal activity is reduced. More preferably, it does not exceed 40 degreeC. The concentrate thus obtained is lyophilized using a lyophilizer, whereby lyophilization can prevent loss of the active ingredient over time. Thus obtained ginseng root extract of the present invention has a high control effect on the moth as confirmed through the examples.

In addition, in the present invention, there is provided a moth-prepared composition comprising the ginseng root extract obtained as above as an active ingredient. The composition may further comprise an aqueous solution of 5-15% ethanol in an amount capable of diluting the Gosam root extract by 100 to 2,000 times. Preferably, after diluting the ginseng root extract of the present invention in an ethanol aqueous solution, a surfactant serving as an electrodeposition agent is added at 0.1 to 2% by weight. As the surfactant, a nonionic surfactant is preferable. For example, TWEEN-80 (PEO (20) -Sorbitan Monooleate), TWEEN-60 (PEO (20) -Sorbitan Monostearate), etc., which have been confirmed to be safe to human body, may be used. Can be. In addition, the present composition contains nimtri extract with azadiractin as an active ingredient, deris extract with rotenone as an active ingredient, and tusendanin for enhancing insecticidal effects or expanding the insecticidal range. Known plant extracts known in the insecticidal effect, such as mulberry extract as an active ingredient may be further included. In addition, the ginseng root extract of the present invention can be used as a compound insecticide in combination with other insecticide components as well as moths.

In addition, the present invention provides a method for controlling moths, including diluting the ginseng root extract of the present invention 100 to 2,000 times in 5 to 15% aqueous ethanol solution and then spraying the plant. Preferably, the ginseng root extract is diluted 500-1,000 times in 5-15% ethanol aqueous solution, and more preferably, the ginseng root extract is diluted in 10% ethanol aqueous solution. In addition, preferably, the ginseng root extract of the present invention is diluted in an ethanol aqueous solution, and then, a surfactant (TWEEN 80, etc.), which serves as an electrodeposition agent, is added in an amount of 0.1 to 2% by weight to evenly spread the entire leaf surface of the plant.

The ginseng root extract obtained according to the present invention can be used as an effective moth-proofing agent in place of chemical pesticides that have been used since it shows excellent moth-preserving activity of 80% to 90% or more, as it is confirmed through the examples. have. Moths using the ginseng root extract of the present invention can solve the soil pollution, toxicity problems to the human body, it can be effectively used in the moths have shown resistance to chemical pesticides. In addition, the ginseng root extract of the present invention can be used as a compound insecticide in combination with other insecticide components.

Hereinafter, the present invention will be described in more detail with reference to specific examples and test examples. However, these examples are only for illustrating the present invention in more detail, the scope of the present invention is not limited by these examples.

Example  One

Red ginseng root  Preparation of Extract

(1) The dried ginseng root was dried to 5% or less of water content, and then ground to a size of 1 to 2 mm.

(2) 100 g of ground ginseng root was put in 1,000 ml of 95% ethanol and extracted for 48 hours in a dark state at room temperature.

(3) The obtained extract was filtered through filter paper and concentrated completely at 40 ° C. using a vacuum concentrator.

(4) The concentrated extract was dried in a lyophilizer to obtain the ginseng root extract of the present invention.

Test Example

Red ginseng root  Extract Cotton moth  Control effect test

(1) First, the ginseng root extract prepared in Example 1 was diluted 500 times and 1,000 times in a 10% ethanol aqueous solution to prepare a solution to be sprayed (“Ginseng root extract diluent”).

(2) When the Chinese cabbage moth ( Plutella xylostella ) was formed to some extent, the pre-density was investigated, and then the distilled root extract of Ginseng root was sprayed and the control value was examined after 3 and 7 days. The test was carried out in the cabbage field prepared according to the general practice, and no pesticide was applied at the test packaging before and after the test period, and the planting distance was 75 × 40 cm. With 6 test spheres, 1, 3 and 5 spheres treated with 1,000-fold dilutions, and 2, 4 and 6 spheres treated with 500-fold dilutions. In addition, 1 and 2 balls (Test Examples 1 and 2) were tested in one repetition, 3 and 4 balls (Test Examples 3 and 4) were tested in 2 repetitions, and 5 and 6 balls (Test Examples 5 and 6) were 3 tested. Test was repeated. The area per each test port was 20 m 2. The results using the 1,000-fold dilution are shown in Tables 1 and 2 below. Table 1 shows the results of day 3 after drug treatment, and Table 2 shows the results of day 7 after drug treatment.

-Exam period: August 2008

-Test Place: Cheongju, Chungbuk

-Arrangement and area of test strip: 3 repetition of the ingot method

-Target pests: Chinese cabbage moth ( Plutella) xylostella )

-Test crops (varieties): Chinese cabbage (Happy Summer Cabbage)

-Insect Pests: The average density of Chinese cabbage moths was 132.7 before treatment.

Comparative example

Three commercially available plant moth preparations of K, P and G companies using natural extracts as Comparative Examples 1, 2 and 3 were tested in the same manner as in Test Example 5, respectively. Compared with. The results are shown in Table 3 below.

Weakness test

1,000 dilutions and 500-fold dilutions were made in the same manner as the control effect test, and a weakening test was performed using the 1,000-fold dilutions as the reference amount and the 500-fold dilutions as the double dose. Six treatment groups were used in the same manner, 1 and 2 balls were tested in one repetition, 3 and 4 balls were tested in two repetitions, and 5 and 6 balls were tested in three repetitions. The area per each test port was 5 m 2. The survey method was to examine whether there was any apparent weakness in the standard dose and the volume. When there is no degree of weakness at all, it is set as “0”, and when there is any degree, it is divided into 5 stages of “1 to 5” and compared with the non-treated group. Evaluation was performed 3, 5. 7 days after drug treatment. As a result, in all six test groups, no apparent deterioration was observed in the standard dose and the same amount as the untreated group.

Claims (9)

(A) step of obtaining the extract by filtering the ginseng root in 90% ~ 99% ethanol of 5 to 15% by volume 40 to 60 hours in the dark at room temperature and then filtered with a filter paper; (b) concentrating the extract obtained above at 30-43 ° C. with a reduced pressure concentrator; (c) lyophilizing the concentrated extract; manufacturing method of high ginseng root extract for moths comprising a. The method according to claim 1, wherein the gosam root is put in 95% ethanol and extracted for at least 48 hours. Ginseng roots were obtained by adding 90 to 99% ethanol in a volume of 5 to 15% by volume and extracting for 40 to 60 hours in a dark state at room temperature, filtering with a filter paper, and then concentrating at 30 to 43 ° C. using a vacuum condenser and freeze drying. Moth-proof composition comprising the extract as an active ingredient. The moth formulation according to claim 3, further comprising an aqueous 5-15% ethanol solution in an amount capable of diluting the Gosam root extract by 100 to 2,000 times. The method of claim 4, wherein the nymph extract; Deris extract; Moth-prepared composition further comprising at least one of mulberry extract. According to claim 4 or 5, wherein the moth formulation composition further comprises 0.1 to 2% by weight of a surfactant that serves as an electrodeposition agent. Ginseng roots were obtained by adding 90 to 99% ethanol in a volume of 5 to 15% by volume and extracting for 40 to 60 hours in a dark state at room temperature, filtering with a filter paper, and then concentrating at 30 to 43 ° C. using a vacuum condenser and freeze drying. Moth control method comprising spraying the plant after diluting the extract 100 to 2,000 times in 5 ~ 15% ethanol aqueous solution. According to claim 7, wherein the moth control method characterized in that diluting the ginseng root extract 500 to 1,000 times in 5 to 15% aqueous ethanol solution. The method of claim 7 or 8, wherein after diluting the ginseng root extract in an ethanol aqueous solution, 0.1 to 2% by weight of a surfactant serving as an electrodeposition agent is added and sprayed.