KR100834965B1 - Pesticide formulation composed of natural components - Google Patents

Abstract

The present invention relates to a natural insecticidal composition, and more particularly, while containing the extract of fish and red ginseng at the same time, and additionally contains herbal extracts such as cinnabar bark, cheonnyeonja, vulgaris in order to obtain higher activity There is excellent control effect against various crops inhibiting pests, and in particular, the control effect against heating pests such as mite, Chinese cabbage moth, and also relates to a natural pesticidal composition that is harmless to human beings.

Fish, red ginseng, insecticide, Chinese cabbage moth, mites

Description

Pesticide formulation composed of natural components

The present invention relates to a natural insecticidal composition, and more particularly, while containing the extract of fish and red ginseng at the same time, and additionally contains herbal extracts such as cinnabar bark, cheonnyeonja, vulgaris in order to obtain higher activity There is excellent control effect against various crops inhibiting pests, and in particular, the control effect against heating pests such as mite, Chinese cabbage moth, and also relates to a natural pesticidal composition that is harmless to human beings.

Today, environmental pollution such as soil and water pollution caused by the large-scale spraying of various organic synthetic pesticides is a problem, accordingly, research on natural pesticides with low toxicity or no pollution is being actively conducted. In other words, the development of natural pesticides that are harmless to the human body can prevent plant pests and improve the quality and yield of the harvest.

The use of fish and red ginseng as natural pesticides has been intensive since the 1980s (Chinese Patent Nos. 1 176742, 11183902, 1057159, 11194782, 97104754, 1171198). For example, Chinese Patent No. 1171198 discloses natural pesticides containing 0.08% of ginseng alkaloids. In addition, Chinese Patent No. 1,119,478 describes the plant's ginseng and vinegar as raw materials, which is characterized by exerting effect simply by pulverizing and blending the plant, and used for the purpose of controlling vegetables, fruits and cotton pests. In particular, it was limited to the control of cabbage white butterfly larvae, aphids.

However, these conventional techniques have limitations in effectively controlling mites and Chinese cabbage moths, which have recently been classified as heating pests.

The main herbal medicine constituents of the present invention (yu teng) and gosam (ku shen) are well known in Chinese medicine dictionary, Chinese medicine coloring book, People's Republic of China Pharmacopoeia, poisonous Chinese herbal medicine dictionary, poisonous herbal medicine, each of these Herbal medicine has been widely used in general, such as edema, diuretic, antiarrhythmic, etc. caused by various bruises, and has been known to have applied to agriculture because of the insecticidal action. Their action is a modern pathological concept that has the characteristics of excitement and paralysis addiction to the central nerve in general.

The present invention contains the extracts of fish and red ginseng that have been applied as agricultural pesticides in folk medicine, not only to show a broad spectrum of insecticides, but also to realize that the control effect on heating pests is excellent. In addition, if the extract of fish roe and ginseng in addition to extracts of jinryokpi, cheonnyeonja or jinjaja can be maximized the effect.

It is an object of the present invention to provide a natural pesticidal composition having a novel composition which is excellent in controlling pests, in particular, in controlling insect pests.

The present invention is characterized by a natural insecticidal composition containing an effective amount of fish extract and ginseng extract.

In addition, the natural insecticidal composition of the present invention may additionally include one or two or more extracts selected from Goryeopi, Ginseng, and Cheonnyeonri along with the extracts of fish and ginseng.

Briefly summarized the characteristics of the natural plant used as a raw material of the present invention are as follows.

Fishing boats are legumes that use medicinal plants or roots, and are suitable for tropical or subtropical climates. Fishing grows well in a relatively shady environment and is best grown on thick, well-drained land. The main mountain ranges are distributed in Guangdong, Guangxi, Yunnan, Fujian, Hunan, Hainan and Taiwan, which are the southeast of China. Fish roots are very toxic and have insecticidal and poisoning effects. It has the characteristic of killing hundreds of kinds of pests at high speed and it is easily decomposed by light and easily oxidized in the air as measured by the Plant Protection Division of HwaNam Agricultural Academy. It is highly toxic to fish but very weak to mammals.

Ginseng is a dry root of legumes and is produced throughout China. Mainly, Shanxi, Hubei, Henan and Hebei provinces are of high quality. Like warm climates, deep sandy, fertile, well drained sandy or viscous soil. Harvested in spring and autumn, especially in autumn. Dig out the roots, remove the top of the roots and the beard roots, then wash off any mud or sand and dry them in the sun. Sliced fresh roots and dried in the sun are called ginseng slices.

It is a dried fruit of soft fruits, and is distributed in Sichuan, Hubei, Guizhou, Henan, Anhui, Jiangsu, etc., and is spherical in shape, 2 to 3.2 cm in diameter, and yellow to brownish yellow. It is slightly glossy and has a small number of depressions or squatters, and it is divided into 6 to 8 parts, each of which contains brown ovoid seeds. The smell is unusual and the taste is sour.

Cinnabar is the bark of dried trees of lotus and plant lotuses. Its main producing areas are Sichuan, Hubei, Anhui, Jiangsu, Henan and Guizhou, and are also produced in Shandong and Yunnan. Cinnamon has a bitter taste of tosendarin. Apparently, it is irregular flat plate shape and the shape of the cylinder and its width is not constant. It has alternating vertical wrinkles and a brownish-brown shell hole. When the rough shell is removed, the inside is white or yellowish white.

A cultivar belongs to a lotus plant, with lush branches and leaves, with high tree trunks, and is a blue tropical plant. It is characterized by rapid growth, withstanding drought high temperatures and harsh conditions, and as the tree trunk grows high, the roots dig deeper. It is native to India, Myanmar and Pakistan, and is also transplanted to Thailand, Iran, Dominica and China. There is an active agent called azadirachtin in the vulgaris and is known to have various effective functions.

All natural plants described above should be prepared and used according to standard methods. First of all, foreign matters or contaminants are removed, and they are chopped or pulverized for easy extraction. Extraction method was used by dividing non-polar solvent and polar solvent conditions according to the characteristics of the herbal medicine, unlike the conventional method alone or all herbal mixture extraction method.

To prepare a fish extract, fish is extracted using a conventional non-polar organic solvent. Typically, benzene, toluene, xylene, cyclohexane, n-nucleic acid, n-pentane, n-heptane, dichloromethane, chloroform, tetra Conventional solvents such as chloromethane can be used alone or in combination. However, the choice of solvent is to maximize the extraction efficiency of the effective pesticides contained in fish, so use carefully selected. In addition, the extraction time and temperature are extracted, filtered and concentrated three times for 7 hours at a temperature of 80 ° C. or less without thermal decomposition of the active ingredient. The extraction method used in the present invention is sufficient according to the traditional method.

On the other hand, the ginseng extract was extracted with a conventional polar organic solvent, typically methanol, ethanol, isopropanol, n-propanol, benzyl alcohol, propyl cellulose, gamma- butyrolactone, N-methyl-2- Conventional solvents such as pyrrolidone, methyl naphthalene and fatty acid methyl ester can be used alone or in combination. However, the choice of solvent has a great effect on the extraction efficiency of the effective pesticides contained in the ginseng, so it should be carefully calculated and used. In addition, extraction time and temperature are extracted, filtered and concentrated three times at five hours or less at a temperature of 80 ° C. or less without thermal decomposition of the active ingredient. The extraction method used in the present invention was sufficient according to the traditional method.

In addition, it was extracted according to the characteristics of the lotus bark, ginyeo jinja, cheonryun, the same as the extraction process of the extract of fish, and cheonyeonja was extracted in the same manner as the extract of Gosam.

Natural insecticidal composition according to the present invention 20 to 80% by weight of fish extract (based on 3.0% index component rotenone content) and 20 to 80% by weight of red ginseng extract (based on 0.2% indicator component matriline alkaloid content) in the total composition It is included. In addition, the natural insecticidal composition of the present invention may be selectively contained in the range of 0 to 30% by weight, in addition to the fish extract and the ginseng extract, cinnabar peel, vulgaris or cheonnyeonja (based on 7.0% X content) in the total composition. In the composition of the natural insecticidal composition of the present invention, when the content of the fish extract is less than 20% by weight or more than 80% by weight, it is difficult to simultaneously control the mite and the Chinese cabbage moth, which is a heating pest. Even if the content of the ginseng extract is less than 20% by weight or more than 80% by weight, it is difficult to simultaneously control the mites and the Chinese cabbage moths, which are heating pests. In addition, even if it does not contain the additionally included bark bark, ginja or cheonngija, even if the extract of fish and red ginseng can obtain a sufficient pesticidal effect, but excellent insecticidal activity, especially excellent control against heating pests such as mite, Chinese cabbage moth For the purpose of effect, it is preferable to include as an essential ingredient, if the content is contained in excess of 30% by weight in the total composition, rather there is a limit to exhibit the unique control properties of fish roe and ginseng.

On the other hand, in the case of herbal medicines obtained from natural plants, the content of the active ingredient varies greatly depending on the origin, collection time, storage period and storage condition. For each extract, the indicator material is selected and the composition ratio is used as the content of the indicator material. It is more preferable to limit. In the present invention, rotenone was selected as an indicator of fish, and an alkaloid matrine was selected as an indicator of red ginseng. Plants were selected by their X content.

Therefore, the natural pesticidal composition of the present invention can limit its composition as the ratio of the indicator material and the X content, and the natural pesticidal composition according to the present invention is based on 100 g of the total composition of 0.6 to 2.4 g of rotenone and 0.04 to martine. 0.16 g and the X content of additional softwoods are contained 0 to 2.1 g. In constructing the natural pesticidal composition of the present invention, the reason for limiting the content ratio of each active active ingredient is the same as the reason for limiting the above-mentioned natural plant extract.

As described above, the natural insecticidal composition according to the present invention can obtain excellent insecticidal effect by containing the fish extract (rotenone) and red ginseng extract (martrin), in addition to the vulgaris, vulgaris or The insecticides were able to maximize the insecticidal effect. Especially, the control effect of mite and Chinese cabbage moth, which is classified as a heating pest, was excellent.

According to the present invention as described above, natural plant extracts were obtained according to the following preparation examples, and natural insecticidal compositions were prepared according to the following examples. Activity was measured. In addition, the following Preparation Examples and Examples as an embodiment, the present invention is not limited by these Preparation Examples and Examples.

Preparation Example 1 Preparation of Fish Extract

After washing with water to remove contaminants, chopped and crushed the dried fish, 440 g, 1,365 ml toluene was added, and the mixture was incubated at 50 ° C for 7 hours, and then refluxed at 70 ° C for 10 hours. The filtrate was collected and the residue was added to 1,090 ml of toluene and re-extracted for 6 hours at 70 ° C. The filtrate was again collected and 1,000 ml toluene was added to the residue and extracted at 70 ° C for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 2 Preparation of Ginseng Extract

After mixing 58 g of red ginseng sliced at 2.0 to 4.0 cm evenly, the mixture was incubated at 410 ml of 90% ethanol for 14 hours at 50 ° C, and then refluxed at 70 ° C for 3 hours. The filtrate was collected and the residue was re-extracted at 70 ° C. for 2 hours by adding 275 ml of 90% by volume ethanol to the residue, and the filtrate was extracted again at 70 ° C. for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 3 Preparation of Cinnamon Extract

After washing with water to remove contaminants, finely dried dried pulp was chopped and pulverized, 440 g of 1,365 ml toluene was added thereto, the mixture was warmed at 50 ° C for 7 hours, and then refluxed at 70 ° C for 10 hours. The filtrate was collected and the residue was added to 1,090 ml of toluene and re-extracted for 6 hours at 70 ° C. The filtrate was again collected and 1,000 ml toluene was added to the residue and extracted at 70 ° C for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 4: Preparation of the extract

After washing with water to remove contaminants, the dried dried finer was chopped and pulverized, 440 g of 1,365 ml of toluene was added thereto, the mixture was warmed at 50 ° C for 7 hours, and then refluxed at 70 ° C for 10 hours. The filtrate was collected and the residue was added to 1,090 ml of toluene and re-extracted for 6 hours at 70 ° C. The filtrate was again collected and 1,000 ml toluene was added to the residue and extracted at 70 ° C for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 5 Preparation of Cheonnyeonja Extract

58 g of thin ginseng sliced at about 2.0 to 4.0 cm was evenly mixed, and after immersing in 410 ml of 90% ethanol for 14 hours at 50 ° C, the mixture was refluxed at 70 ° C for 3 hours. The filtrate was collected and the residue was re-extracted for 2 hours at 70 ° C. with 275 ml of 90% by volume ethanol, and the filtrate was further extracted at 70 ° C. for 2 hours with respect to the residue. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 6 Preparation of Fish Extract

1,365 ml of toluene was added to 440 g of dried fish and 15 g of dried pulp, which was then incubated at 50 ° C. for 7 hours, followed by extraction at 70 ° C. for 10 hours. The filtrate was collected and the residue was added to 1,090 ml of toluene and re-extracted for 6 hours at 70 ° C. The filtrate was again collected and 1,000 ml toluene was added to the residue and extracted at 70 ° C for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 7 Preparation of Mixture of Fish and Fuchsia

440 g of dried fish and 58 g of phosphorus were added to 1,365 ml toluene, and the mixture was warmed at 50 ° C for 7 hours, and then refluxed at 70 ° C for 10 hours. The filtrate was collected, the residue was added to 1,090 ml of toluene and re-extracted for 6 hours at 70 ° C. The filtrate was again collected and 1,000 ml toluene was added to the residue, followed by extraction at 70 ° C for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 8 Preparation of Mixture of Fish, Porridge and Cinnamon

1,365 ml of toluene was added to 440 g of dried fish, 58 g of dried fish and 15 g of red pepper, and the mixture was refluxed at 70 ° C. for 7 hours and then refluxed at 70 ° C. for 10 hours. The filtrate was collected, the residue was added to 1,090 ml of toluene and re-extracted for 6 hours at 70 ° C. The filtrate was again collected and 1,000 ml toluene was added to the residue, followed by extraction at 70 ° C for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Preparation Example 9 Preparation of Red Ginseng and Stellar Extract

Mix 58 g of red ginseng, cut into 2.0 to 4.0 cm, and 30 g of pulverized pulverum so as to break up the seeds of the seed. Then, incubate at 50 ° C for 14 hours in 410 ml of 90% ethanol, and reflux at 70 ° C for 3 hours. It was. The filtrates were collected, 275 mL of 90% ethanol was added to the residue, and the mixture was re-extracted for 6 hours at 70 ° C., and the filtrates were further extracted at 70 ° C. for 2 hours. The filtrates were combined and concentrated to a 210 ml volume.

Reference Example: Selection of Indicators

HPLC analysis of the rotenone compound, the index of fish extract, was carried out using Waters' solvent gradient pump. As a solvent, 0.1N aqueous solution of phosphoric acid / acetonitrile was started at 90:10 ratio and up to 90 minutes. The gradient was applied to a ratio of 0: 100. The column was Phenomenex C ₁₈ and the ultraviolet detector was measured at 295 nm. Samples were injected 10 microns each through an auto sampler. The results were analyzed by the company's Autochro software. In addition, the alkaloid materin, which is a high ginseng indicator, was added 1.6203 g of a sample to a separatory filter, added to 2 ml of concentrated ammonia water, and 10 ml of chloroform was added thereto. After three repeated extractions, the mixture was evaporated in a water bath. The residue was evaporated again using 3 ml of neutral anhydrous ethanol, 10 ml of ethyl ether was added, dissolved, and 10 ml of 0.01 M sulfuric acid solution was added to the ether. After cooling, 15 ml of pure water was added and titrated with 0.0176 M sodium hydroxide solution together with 2-3 drops of methyl orange indicator. In the blank test, 10 ml of 0.01 M sulfuric acid solution and 15 ml of pure water were added, and 2 to 3 drops of methyl orange were carried out.

The indicator substances selected by the above method are as follows.

Example: Preparation of Natural Insecticidal Compositions

Each natural plant extract obtained by the method of Preparation Example was mixed in the ratio as shown in Table 1 to prepare a natural pesticidal composition. At this time, polyoxyethylene sorbitan monooleate ("emulsifier 1", Koremul SFT-85 superconcentrate) and polyoxyethylene octyl phenyl ether ("emulsifier 2" Koremul OP-10) as standard emulsifiers for uniform emulsification of the composition Hwaseong) was used each 4% by weight based on the total amount of the herbal extracts described above. In addition, industrial ethanol (Jin Chemical Pharmaceutical Co., Ltd.) was used as a solvent for matching the percentage of the composition.

division Example (% by weight) One 2 3 4 5 Fish extract 45 - - - 65 Ginseng extract - 45 - - - Fishery, Cinnamon Extract - 45 - - - Fishing boat, vulgaris extract - - 45 - - Effervescent, red pepper, vulgaris extract - - 45 - Red Ginseng, Cinnamon Extract 45 - 45 45 25 Emulsifiers 1 6.0 6.0 6.0 6.0 6.0 Emulsifier 2 4.0 4.0 4.0 4.0 4.0

division Comparative example (% by weight) One 2 3 4 5 6 Fish extract 90 - - - - 15 Ginseng extract - 90 - 80 - 15 Cinnabar Extract - - 90 - - 10 Phosphorus extract - - - 10 - 15 Puncture extract - - - 90 35 Emulsifiers 1 6.0 6.0 6.0 6.0 6.0 6.0 Emulsifier 2 4.0 4.0 4.0 4.0 4.0 4.0

Experimental Example:

In order to compare the activity of the natural insecticidal composition prepared in the composition of Table 1a and Table 1b for the efficacy of the following experiment was carried out.

Experimental Example 1: Test result of spotted mite

Two adult spotted spider mite (TSSM; Tetranychus urticae) were caught on a 3 cm diameter Gangnam leaf disc, sprayed with a constant solution in a hood and tested for insecticide after 24 and 48 hours, respectively. It is shown in Table 2 below.

Experimental Example 2: Chinese cabbage moth drug efficacy test results

Soak cabbage leaves of 5 cm in diameter for 30 seconds in the prepared solution, dry them in a hood, and inoculate 20 3 larvae of Chinese cabbage moth (DBM; Diamond-back moth, plutella xylostella) each for 24 hours and 48 hours. After the passage of time, the insecticidal rate was assayed and shown in Table 3 below.                     

Spotted Mite Drug Test Results (% Pesticide) division Elapsed time Treatment concentration (dilution drainage) 50 times 100 times 500 times Control (untreated) 24 hours 0 0 0 48 hours 0 0 0 Example 1 24 hours 90 10 10 48 hours 100 25 10 Example 2 24 hours 95 10 0 48 hours 100 10 0 Example 3 24 hours 100 85 80 48 hours 100 100 90 Example 4 24 hours 86 30 10 48 hours 95 55 10 Example 5 24 hours 95 35 25 48 hours 100 45 30 Comparative Example 1 24 hours 20 0 0 48 hours 25 0 5 Comparative Example 2 24 hours 0 0 0 48 hours 0 0 0 Comparative Example 3 24 hours 45 20 5 48 hours 60 25 5 Comparative Example 4 24 hours 100 20 10 48 hours 100 45 25 Comparative Example 5 24 hours 20 5 5 48 hours 50 5 5 Comparative Example 6 24 hours 40 35 10 48 hours 75 40 15

Chinese cabbage leaf moth drug test result (% insecticide) division Elapsed time Treatment concentration (dilution drainage) 100 times 500 times 1,000 times Control (untreated) 24 hours 0 0 0 48 hours 0 0 0 Example 1 24 hours 100 75 50 48 hours 100 85 60 Example 2 24 hours 100 95 60 48 hours 100 100 85 Example 3 24 hours 100 60 50 48 hours 100 65 50 Example 4 24 hours 90 55 10 48 hours 100 70 30 Example 5 24 hours 95 100 85 48 hours 100 100 95 Comparative Example 1 24 hours 100 45 5 48 hours 100 55 15 Comparative Example 2 24 hours 70 45 5 48 hours 85 50 15 Comparative Example 3 24 hours 50 25 10 48 hours 95 30 10 Comparative Example 4 24 hours 80 40 15 48 hours 85 60 15 Comparative Example 5 24 hours 80 25 0 48 hours 80 25 0 Comparative Example 6 24 hours 75 10 0 48 hours 90 35 5

As described above, the natural insecticidal composition of the present invention is composed of herbal extracts, which are completely harmless to human beings, but have excellent control effects against various crops inhibiting pests, and particularly, control against heating pests such as mites and Chinese cabbage moths. As it is excellent, it is useful as a next generation pesticide.

Claims (7)

Natural insecticidal composition containing an effective amount of fish extract and ginseng extract. The natural insecticidal composition according to claim 1, wherein 20 to 80% by weight of the fish extract and the ginseng extract are contained. The natural insecticidal composition according to claim 1 or 2, wherein 0.6 to 2.4 g of rotenone and 0.04 to 0.16 g of matrin are contained as an indicator substance based on 100 g of the composition. The natural insecticidal composition according to claim 1, wherein said composition further contains extracts of one or two or more softwoods selected from bark bark, bark bark, or bark. 5. The natural insecticidal composition according to claim 4, wherein the extract of the soft fruit is contained in the total composition in a range of more than 0% to 30% by weight. The method according to claim 4 or 5, based on 100 g of the composition, the content of 0.6 to 2.4 g of rotenone, 0.04 to 0.16 g of matriline, and the X content of additional hardened bark, physician or vulgaris, respectively, 0 to 2.1. natural insecticidal composition, characterized in that it contains g. The natural insecticidal composition according to any one of claims 1, 2, 4 or 5, which is useful for controlling pests of a heating agent selected from mites and Chinese cabbage moths.