CN106490070A - A kind of biological insecticides of preventing and treating rice leaf roller and preparation method thereof - Google Patents
A kind of biological insecticides of preventing and treating rice leaf roller and preparation method thereof Download PDFInfo
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- CN106490070A CN106490070A CN201610926214.0A CN201610926214A CN106490070A CN 106490070 A CN106490070 A CN 106490070A CN 201610926214 A CN201610926214 A CN 201610926214A CN 106490070 A CN106490070 A CN 106490070A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
Abstract
The invention provides a kind of biological insecticides of preventing and treating rice leaf roller, belong to technical field of pesticide.A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Aglaia odorata spend 10 ~ 30 parts, 10 ~ 30 parts of the fruit of Rangoon creeper, 5 ~ 20 parts of needle juniper, white drench 5 ~ 20 parts of grass, 5 ~ 20 parts of cuckoo meat, 5 ~ 15 parts of Inula britannica chinensis, 3 ~ 12 parts of vernonia anthelmintica, 3 ~ 12 parts of elm bark, 3 ~ 12 parts of the tuber of stemona, 5 ~ 10 parts of allicin, 1 ~ 5 part of bacteria agent, the bacteria agent is by 25 ~ 35% thuringiensis of mass percent, 30 ~ 40% bacillus licheniformis and 35% ~ 45% Streptomyces jingyangensis.Insecticide of the present invention is drawn materials environmental protection, to paddy rice without any harm, and efficiently can kill rice leaf roller, and disease-resistant rate can reach 100%, and Long-Time Service is without the resistance to the action of a drug.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of biological insecticides of preventing and treating rice leaf roller and its preparation
Method.
Background technology
Rice leaf roller [Cnaph alocrocis medinalis (Guenee)] belongs to Lepidoptera, and Pyralidae is the southeast
A kind of migratory pest of sub- and Northeast Asia hazard rice.Host in addition to paddy rice, also does not have found to take food and completes under field conditions (factors)
Other plants of generation.Rice leaf roller mainly with larva cause harm rice leaf affect Rice Population growth and yield：From tiller
Phase to heading stage, newly hatched larvae take food epicuticle and green meat leaf texture in the tender leaf sheath seam near lobus cardiacus or lobus cardiacus, enter
Climb to after two ages near blade tip, spinning knot volume rice leaf is ensconced in bud and takes food meat leaf into bud, with turning strain Damage habits, appetite with
Age increases and increases.Gnaw blade and form white bars spot, cause the minimizing of photosynthetic area and dry biomass, affect light cooperation
With；Aggrieved heavy rice field is a piece of withered white, has a strong impact on the quality and yield of paddy rice.
Rice leaf roller from 2003 since national outburst, due to different geographical cropping system, benign climate, host
Characteristic etc. that abundant, natural enemy pest controlling ability declines, pesticide application technology is poor, the generation of the anti-medicine that cures mainly medicament and insect migrate multiple because
Element affects, and causes the insect outburst frequency to increase, maintains higher level always.For a long time, people are mainly using chemical agriculture
Medicine controls diseases and pests of agronomic crop.Due to the long-term a large amount of and Reusability of chemical pesticide, dirt is generated to soil, water body and air
Dye, make agricultural byproducts Pesticide Residues increase, also directly compromise the mankind health and existence and insect to chemical pesticide
The resistance to the action of a drug is also being improved year by year, while the cost accounting more and more higher chemical pesticide control insect of crops, has also killed day
Enemy and other beneficial organisms, destroy the ecological balance.Progress, expanding economy, Environmental security and food with science and technology and society
Product become safely the key subjects that the whole mankind pays close attention to jointly.High poison, the production of high residue chemical pesticide and use is limited, is pushed greatly
Extensively using safety, low toxicity, the biological insecticides of environment-friendly type, a developing direction is had become.
Biological insecticides be directly using biogenic bioactivator or biological living mycelium, half armful of crystal,
Insect viruses etc. as agricultural chemicals, including microbial insecticide, farm antibiotics, botanical pesticide, biochemical pesticides, transgenosis
Biological insecticides and natural enemy biological insecticides.Compared with chemical pesticide, biological insecticides have the advantage that：1. biological insecticides
Toxicity generally low than chemical pesticide, to person poultry safety, great majority have no toxic side effect；2. selective strong, specially to harmful organism mark
Target, does not kill pest natural enemy, is not for non-target organism, is " protection type agricultural chemicals "；3. low-residual, efficiently.On the whole can be with
Avoid the problem of environmental pollution that chemical pesticide brings；4. the resistance to the action of a drug is not likely to produce, long service life, dispenser cost are low, is " effect
Benefit agricultural chemicals "；5. as a constituent of integrated pest control, the usage amount of chemical pesticide can be greatly reduced, and not
Affect crop yield.
China's living resources are enriched, and have the Biological Genetic Resources in the whole world, provide favourable bar for developing biological insecticides
Part.The patent of Application No. 200710053522 discloses a kind of plant powder pesticide and preparation method thereof, its parts by weight
Plant material：Macleaya cordata 200-300, wolf's milk 300-400, polygonum flaccidum 200-300, unpeeled RADIX GLYCYRRHIZAE 50-150, Chinese atropanthe root 300-400,
Corter pseudolaricis 150-250, root bark of tree peony 200-300, hairyvein agrimony 150-250, short-tube lycoris 150-250.The invention is to rice leaf roll worm, dark greyish green
Worm, aphid have preferable lethal effect., more than 96%, the death rate is more than 80% for anti-food rate to insect.Application No.
201310302262 patent discloses a kind of rice leaf roller PuGV Dipel biological insecticides and preparation side
Method, the biological insecticides agent is by rice leaf roller PuGV, Dipel, sodium lignin sulfonate, methylene dinaphthalene sulphur
Sour sodium and kaolin are formulated by a certain percentage.The invention insecticidal spectrum is wide, instant effect, product stability are good, to environment and people
Poultry safety, noresidue, can effective control rice leaf roller generation and harm, be suitable to be applied on paddy rice in a large number, particularly without
Apply on public hazards rice and organic rice.Technology disclosed above shows that biogenic insecticide is in paddy rice rice leaf roller
There are in terms of preventing and treating certain potential advantages, consistent with the long-range target that China advocates development pollution-less agriculture.Meanwhile, we
It should also be recognized that, China's living resources are enriched, and many potential resources need to be developed, and are killed in biology with giving full play to China
Resources advantage in terms of worm agent particularly Farm chemical of Chinese berbs.It should be noted that while biological insecticides are evolving, also depositing
Which is annoying in some distinct issues and difficulty to advance, such as biological insecticides knock down speed slowly, and the term of validity is shorter, phase
Narrower to insecticidal spectrum, not strong to pest population controling power in a short time, it is impossible to rapid reduction insect insect density etc..
Content of the invention
In view of this, the technical problem to be solved is to provide a kind of Biocidal of preventing and treating rice leaf roller
Agent, insecticidal effect are stable, knock down speed to insect soon, and control time is long.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Aglaia odorata spends 10 ~ 30
Part, 10 ~ 30 parts of the fruit of Rangoon creeper, 5 ~ 20 parts of needle juniper, 5 ~ 20 parts of white pouring grass, 5 ~ 20 parts of cuckoo meat, 5 ~ 15 parts of Inula britannica chinensis, vernonia anthelmintica
3 ~ 12 parts, 3 ~ 12 parts of elm bark, 3 ~ 12 parts of the tuber of stemona, 5 ~ 10 parts of allicin, 1 ~ 5 part of bacteria agent, the bacteria agent is by quality
25 ~ 35% thuringiensis of percentage, 30 ~ 40% bacillus licheniformis and 35% ~ 45% Streptomyces jingyangensis.
Preferably, the bacteria agent by 30% thuringiensis of mass percent, 35% bacillus licheniformis and
35% Streptomyces jingyangensis.
Preferably, the biological insecticides of the preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice is young
20 ~ 25 parts of orchid, 15 ~ 20 parts of the fruit of Rangoon creeper, 8 ~ 12 parts of needle juniper, white drench 8 ~ 12 parts of grass, 8 ~ 12 parts of cuckoo meat, 8 ~ 12 parts of Inula britannica chinensis,
6 ~ 8 parts of vernonia anthelmintica, 6 ~ 8 parts of elm bark, 6 ~ 8 parts of the tuber of stemona, 6 ~ 8 parts of allicin, 2 ~ 3 parts of bacteria agent.
Preferably, the biological insecticides of the preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice is young
25 parts of orchid, 15 parts of the fruit of Rangoon creeper, 12 parts of needle juniper, 8 parts of white pouring grass, 12 parts of cuckoo meat, 12 parts of Inula britannica chinensis, 8 parts of vernonia anthelmintica, elm
6 parts of bark, 6 parts of the tuber of stemona, 8 parts of allicin, 2 parts of bacteria agent.
A kind of process for preparing bio-insecticide of preventing and treating rice leaf roller, comprises the following steps：
1）The Aglaia odorata for weighing dry weight portion respectively spends 10 ~ 30 parts, in vain 10 ~ 30 parts of the fruit of Rangoon creeper, pouring careless 5 ~ 20 parts, cuckoo meat
3 ~ 12 parts of 5 ~ 20 parts, 3 ~ 12 parts of vernonia anthelmintica, 3 ~ 12 parts of elm bark and the tuber of stemona, mixing are carried out after freezing under nitrogen protection
Crush, obtain comminuting matter I；The needle juniper 5 ~ 20 part and Inula britannica chinensis 5 ~ 15 part of dry weight portion are weighed respectively, and mixing is crushed,
Obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 ~ 3 times of its weight, it is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 medicinal extract, adds volume fraction in the medicinal extract
For 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, kept for 18 ~ 24 hours, then filter, obtain filtrate III and sediment, by institute
State the lotion that relative density when filtrate III is evaporated to 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with organic solvent, sub-cloud solution, vacuum revolving instrument steams organic solvent, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain active ingredient I；
5）The active ingredient I is mixed with 5 ~ 10 weight portion of allicin, 1 ~ 5 weight portion of bacteria agent, 2000 ~ 3000rpm is stirred
Mix 5 ~ 10 minutes, obtain final product.
Preferably, the w/v 1g of the medicinal extract and the ethanol：10~20ml.
Preferably, the organic solvent is dichloromethane.
1）1）Present invention applicant is on the basis of studying to Rice Cropping, it is proposed that a kind of life of preventing and treating rice leaf roller
Thing insecticide, the biological insecticides can efficiently kill rice leaf roller to paddy rice without any harm, and killing rate reaches 100%,
Long-Time Service is without the resistance to the action of a drug.Meanwhile, the resistance against diseases that the crops such as paddy rice can also be strengthened using the biological insecticides.Remove paddy rice
Can be beyond use, other can also be used by the crops of rice leaf roller harm.Biological insecticides of the present invention are multiple by science
With acquisition, in practice it has proved which can not only effectively kill insect pest, and can feed corps nutrient, resistance against diseases is improved, shown
Writing reduces the probability for suffering from insect pest of paddy rice.
2）The raw materials used wide material sources of the present invention, safety and environmental protection.Aglaia odorata is spent, another name：Sieve flower, Milan flower, tree orchid is patrolled,
Gas delicate fragrance, includes multiple volatile oil components.The fruit of Rangoon creeper, another name：Scoop ask son,History gentleman, four gentleman,Combretaceae、The fruit of Rangoon creeper belongs to
Climb up by holding on to shape shrub.Needle juniper, is the branches and leaves and cone of cupressaceae plant needle juniper, includes multiple volatile oil components.White pouring grass, is Urticaceae
The herb of plant Herba Pileae longicaulis.Cuckoo meat, another name：RedCuckoo, quick-fried caneCuckoo, bamboo locust, oryza chinensis, mythimna separata, lice, software can be killed and moved
Thing etc., can also prevent and treat the late blight of potato.Inula britannica chinensis, also known as：Jin Fohua, goldBuddhist grassDeng belonging to composite family, Inula perennial
Draft.Vernonia anthelmintica, is the fruit of feverfew vernonia anthelmintica, containing multiple volatile oil components.Elm bark, beUlmaceaePlant
The dry bark or root skin of elm Ulmus pumila L., can be used for urinary obstruction, stranguria with turbid discharge, oedema, ulcer carbuncle on the back, erysipelas, scabies
Tinea.The tuber of stemona, is the root of Stemonaceae plant radix stemonae sessilifoliae, Radix stemonae japonicae and radix stemonae tuberosae.Above-mentioned raw materials are applicants through for many years
Research, screening, compound gained through science；And be used in combination with bacteria agent, help each other, growth week whole to paddy rice
The rice leaf roller that phase occurs has obvious prevention effect, after the absorbable utilization of paddy rice, is conducive to the degeneration-resistant energy for improving paddy rice
Power, and insecticide of the present invention does not have any injury to non-target object.
Specific embodiment
For a better understanding of the present invention, present disclosure, but this are further fairly set out with reference to embodiment
Bright protection content is not limited solely to the following examples.In the following description, give a large amount of concrete details so as to
More thorough understanding of the invention is provided.It will be apparent, however, to one skilled in the art that the present invention can be with
It is carried out without the need for one or more of these details.
Embodiment 1：A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice
25 parts of young orchid, 15 parts of the fruit of Rangoon creeper, 12 parts of needle juniper, white drench 8 parts of grass, 12 parts of cuckoo meat, 12 parts of Inula britannica chinensis, 8 parts of vernonia anthelmintica,
6 parts of elm bark, 6 parts of the tuber of stemona, 8 parts of allicin, 2 parts of bacteria agent, 30% Su Yun gold bud of the bacteria agent by mass percent
Born of the same parents bacillus, 35% bacillus licheniformis and 35% Streptomyces jingyangensis.
Bacteria agent used by of the invention, thuringiensis, bacillus licheniformis and Streptomyces jingyangensis can pass through market
Channel is commercially available.Wherein, thuringiensis, Bacillus thuringiensis, living bacteria count >=20,000,000,000/gram；
Bacillus licheniformis, Bacillus licheniformis, living bacteria count >=1,000,000,000/gram；Streptomyces jingyangensis,
Streptomyces Jingyangensis, living bacteria count >=200,000,000/gram.
A kind of process for preparing bio-insecticide of preventing and treating rice leaf roller, comprises the following steps：
1）Dry above-mentioned Aglaia odorata flower, the fruit of Rangoon creeper, Bai Lincao, cuckoo meat, vernonia anthelmintica, elm bark and hundred are weighed respectively
Portion, mixing, is crushed after freezing under nitrogen protection, obtains comminuting matter I；Dry above-mentioned needle juniper and sheathed is weighed respectively
Flower, mixing are crushed, and obtain comminuting matter II；
2）The water of 5 times of its weight is added in the comminuting matter I, 50 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 times of its weight, 55 DEG C are heated to, are kept
35 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.13 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
28 DEG C, kept for 24 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.23 lotion；
3）The water of its 4 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 38 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain active ingredient I；
5）The active ingredient I is mixed with allicin, bacteria agent, 2500rpm is stirred 8 minutes, is obtained final product.
The medicinal extract is 1g with the w/v of the ethanol：16ml.
The using method of biological insecticides of the preventing and treating rice leaf roller is：Add the biological insecticides insecticide weight
The water that more than 500 times of amount, is thoroughly mixed, makes spraying agent.
Embodiment 2：A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice
25 parts of young orchid, 15 parts of the fruit of Rangoon creeper, 12 parts of needle juniper, white drench 8 parts of grass, 12 parts of cuckoo meat, 12 parts of Inula britannica chinensis, 8 parts of vernonia anthelmintica,
6 parts of elm bark, 6 parts of the tuber of stemona, 7 parts of allicin, 3 parts of bacteria agent, 25% Su Yun gold bud of the bacteria agent by mass percent
Born of the same parents bacillus, 30% bacillus licheniformis and 45% Streptomyces jingyangensis.
A kind of process for preparing bio-insecticide of preventing and treating rice leaf roller, comprises the following steps：
1）Weigh respectively the Aglaia odorata flower of dry above-mentioned weight, the fruit of Rangoon creeper, Bai Lincao, cuckoo meat, vernonia anthelmintica,
Elm bark and the tuber of stemona, mixing, are crushed after freezing under nitrogen protection, obtain comminuting matter I；Weigh respectively dry above-mentioned
The needle juniper and Inula britannica chinensis of weight, mixing are crushed, and obtain comminuting matter II；
2）The water of 4 times of its weight is added in the comminuting matter I, 40 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 times of its weight, 50 DEG C are heated to, are kept
38 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.15 medicinal extract, adds the ethanol solution that volume fraction is 80%, temperature in the medicinal extract
25 DEG C, kept for 24 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.25 lotion；
3）The water of its 3 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 35 minutes, and distillation is extracted 2 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain active ingredient I；
5）The active ingredient I is mixed with allicin, bacteria agent, 2000rpm is stirred 10 minutes, is obtained final product.
The medicinal extract is 1g with the w/v of the ethanol：12ml.
The using method of the biological insecticides of the preventing and treating rice leaf roller is repeated no more with embodiment 1.
Embodiment 3：A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice
20 parts of young orchid, 20 parts of the fruit of Rangoon creeper, 8 parts of needle juniper, 12 parts of white pouring grass, 8 parts of cuckoo meat, 8 parts of Inula britannica chinensis, 6 parts of vernonia anthelmintica, elm
7 parts of bark, 7 parts of the tuber of stemona, 6 parts of allicin, 3 parts of bacteria agent, 35% Su Yun gold brood cell of the bacteria agent by mass percent
Bacillus, 30% bacillus licheniformis and 35% Streptomyces jingyangensis.
A kind of process for preparing bio-insecticide of preventing and treating rice leaf roller, comprises the following steps：
1）The Aglaia odorata flower of dry above-mentioned weight, the fruit of Rangoon creeper, needle juniper, cuckoo meat, vernonia anthelmintica, elm are weighed respectively
Bark and the tuber of stemona, mixing, are crushed after freezing under nitrogen protection, obtain comminuting matter I；Weigh respectively dry above-mentioned heavy
The white pouring grass of amount part proportioning and Inula britannica chinensis, mixing, crush, obtain comminuting matter II；
2）The water of 6 times of its weight is added in the comminuting matter I, 50 DEG C are heated to, and is kept for 45 minutes, is filtered while hot, obtain
Filtrate I and the dregs of a decoction I, filtrate are cooled down, sealing preserve；In the water that the dregs of a decoction I add 3 times of its weight, 60 DEG C are heated to, are kept
40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and obtains filtrate, by the filtrate
When being concentrated into 75 DEG C, relative density is 1.12 medicinal extract, adds the ethanol solution that volume fraction is 75%, temperature in the medicinal extract
30 DEG C, kept for 20 hours, then filter, obtain filtrate III and sediment, relative when the filtrate III is evaporated to 55 DEG C
Density is 1.21 lotion；
3）The water of its 5 times of weight of weight being added in the comminuting matter II, being added in distilling flask, is distilled, control is steamed
Evaporating speed and the drop of solution 2 ~ 3 being oozed for backflow per second, distillation time is 40 minutes, and distillation is extracted 3 times, by extract merging dichloro
Methane is extracted, sub-cloud solution, and vacuum revolving instrument steams dichloromethane, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain active ingredient I；
5）The active ingredient I is mixed with allicin, bacteria agent, 2500rpm is stirred 5 minutes, is obtained final product.
The medicinal extract is 1g with the w/v of the ethanol：18ml.
The using method of the biological insecticides of the preventing and treating rice leaf roller is repeated no more with embodiment 1.
Embodiment 4：A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice
10 parts of young orchid, 30 parts of the fruit of Rangoon creeper, 5 parts of needle juniper, 5 parts of white pouring grass, 5 parts of cuckoo meat, 15 parts of Inula britannica chinensis, 3 parts of vernonia anthelmintica, elm
12 parts of bark, 10 parts of the tuber of stemona, 5 parts of allicin, 1 part of bacteria agent, 28% Su Yun gold bud of the bacteria agent by mass percent
Born of the same parents bacillus, 32% bacillus licheniformis and 40% Streptomyces jingyangensis.
Embodiment 5：A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice
30 parts of young orchid, 10 parts of the fruit of Rangoon creeper, 20 parts of needle juniper, 20 parts of white pouring grass, 20 parts of cuckoo meat, 5 parts of Inula britannica chinensis, vernonia anthelmintica 10
Part, 3 parts of elm bark, 12 parts of the tuber of stemona, 9 parts of allicin, 4 parts of bacteria agent, 32% Su Yun of the bacteria agent by mass percent
Golden bacillus, 30% bacillus licheniformis and 38% Streptomyces jingyangensis.
Embodiment 6：A kind of biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Rice
15 parts of young orchid, 25 parts of the fruit of Rangoon creeper, 15 parts of needle juniper, 15 parts of white pouring grass, 15 parts of cuckoo meat, 6 parts of Inula britannica chinensis, vernonia anthelmintica 12
Part, 10 parts of elm bark, 3 parts of the tuber of stemona, 10 parts of allicin, 5 parts of bacteria agent, the bacteria agent are revived by the 25% of mass percent
Cloud gold bacillus, 40% bacillus licheniformis and 35% Streptomyces jingyangensis.
Embodiment 7：The biological insecticides of the preventing and treating rice leaf roller described by the present embodiment, as different from Example 1：
The biological insecticides of the preventing and treating rice leaf roller, also include the following raw material of weight portion：1.5 parts of alkyl benzene sulfonate calcium salt, water
0.8 part of 0.2 part of poplar acid sodium and sodium chloride.
Embodiment 8：The biological insecticides of the preventing and treating rice leaf roller described by the present embodiment, different from embodiment 1
It is：The biological insecticides of the preventing and treating rice leaf roller, also include the following raw material of weight portion：1.2 parts of alkyl benzene sulfonate calcium salt,
0.6 part of 0.1 part of sodium salicylate and sodium chloride.
Compared with Example 1, the stability of biological insecticides aqua is higher for comparative example 7 ~ 8, Shelf-life 15 ~ 20 days；
Wettability more preferably, reduces mu consumption 10 ~ 15%.
Comparative example 1：The biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Aglaia odorata
Spend 25 parts, 15 parts of the fruit of Rangoon creeper, 12 parts of needle juniper, white drench 8 parts of grass, 12 parts of cuckoo meat, 12 parts of Inula britannica chinensis, 8 parts of vernonia anthelmintica, elm
6 parts of skin, 6 parts of the tuber of stemona.
Comparative example 2：The biological insecticides of preventing and treating rice leaf roller, are prepared from by the raw material of following weight portion：Aglaia odorata
Spend 25 parts, 15 parts of the fruit of Rangoon creeper, 12 parts of needle juniper, white drench 8 parts of grass, 12 parts of cuckoo meat, 12 parts of Inula britannica chinensis, 8 parts of vernonia anthelmintica, elm
6 parts of skin, 6 parts of the tuber of stemona, 8 parts of allicin
Comparative example 3：The biological insecticides of preventing and treating rice leaf roller, by 30% thuringiensis of mass percent, 35% ground
Clothing bacillus and 35% Streptomyces jingyangensis.
Effect assessment
1.1 experimental design
9 process of field reagent experimental design, respectively：
Experimental group：A1~A6：It is followed successively by the biological insecticides of the preparation of embodiment 1 ~ 2,3 ~ 4,7 ~ 8；
Control group：B1~B3：It is followed successively by the biological insecticides of the preparation of comparative example 1 ~ 3；B0：Non- dispenser is used as blank.
Often process and be repeated 3 times, altogether 30 cells, be 100m per plot area2, random alignment.
1.2 efficacy survey
Determine at 5 points at random per cell, per 10 cave rice strains, after medication, 1,5,12d respectively investigate 1 rice leaf roller of record
Harm leaf roll number, the total plant greenery number in rice cave, after medication, 1,5 d record rice leaf roller always borer population living.The data money that investigation is obtained
Material is calculated as the following formula：
Leaf roll rate/%=leaf rolls number/investigation total number of sheets × 100；
Hundred cave worm amounts/%=always live borer population/investigation rice cave number × 100；
Pest controlling efficiency/%=[check plot leaf roll rate-treatment region leaf roll rate]/check plot leaf roll rate × 100；
Insecticide efficiency/%=[hundred cave worm amount of check plot-hundred cave worm amount for the treatment of region] hundred cave worm amount × 100 of/check plot.
1.3 result
Different agents are shown in Table 1 to the prevention effect of rice leaf roller.
Prevention effect of 1 different agents of table to rice leaf roller
As can be seen from Table 1,1）Pest control effect：For the present invention, in the 1st day for treating, the pest control effect of embodiment A1
Highest, and less with experimental group A2 ~ A6 differences, after reagent, the investigation result of the 5th day and the 12nd day can be seen, experimental group control
Evil lasts are stable, and the effect for being applied to the 1st day is most obvious, and speedup slows down afterwards, illustrates insecticide of the present invention to insect
Control speed ratio is very fast, and effect stability.For comparative example, B1 ~ B3 differs greatly in the prevention effect of the 1st day, and
It is less than 45%；Investigation result the 5th day for treating and the 12nd day shows, prolongation over time, pest control effect percentage speedup
Slowly, final pest control effect is still significantly lower than experimental group.Above-mentioned contrast finds out, control effect of the present invention to rice leaf roller
Stable and notable.
2）Insecticidal effect：Experimental group A1 of the present invention ~ A2, A5 ~ A6 reaches 100%, A3 ~ A4 summaries in the insecticidal effect of the 5th day
Low, respectively 99.5% and 99.1%.And control group to the insecticidal effect at the end of control time is below 60%, and difference is substantially,
Above-mentioned comparative illustration, the insecticidal effect of insecticide of the present invention are notable, and desinsection fruit is quick, and effect stability fully shows this
Bright insecticide Synergistic, is significantly better than that mixture component, and than mixture component preventive effect faster, more stable.
Although the foregoing describing the specific embodiment of the present invention, those familiar with the art should manage
Solution, the specific embodiment described by us are merely exemplary, rather than for the restriction to the scope of the present invention, are familiar with this
Equivalent modification and change that the technical staff in field is made in the spirit according to the present invention, should all cover the present invention's
In scope of the claimed protection.
Claims (7)
1. a kind of preventing and treating rice leaf roller biological insecticides, it is characterised in that：It is prepared from by the raw material of following weight portion：Rice
10 ~ 30 parts of young orchid, 10 ~ 30 parts of the fruit of Rangoon creeper, 5 ~ 20 parts of needle juniper, 5 ~ 20 parts of white pouring grass, 5 ~ 20 parts of cuckoo meat, Inula britannica chinensis 5 ~ 15
Part, 3 ~ 12 parts of vernonia anthelmintica, 3 ~ 12 parts of elm bark, 3 ~ 12 parts of the tuber of stemona, 5 ~ 10 parts of allicin, 1 ~ 5 part of bacteria agent, the life
Thing microbial inoculum is by 25 ~ 35% thuringiensis of mass percent, 30 ~ 40% bacillus licheniformis and 35% ~ 45% Jingyang strepto-
Bacterium.
2. as claimed in claim 1 preventing and treating rice leaf roller biological insecticides, it is characterised in that：The bacteria agent is by matter
30% thuringiensis of amount percentage, 35% bacillus licheniformis and 35% Streptomyces jingyangensis.
3. as claimed in claim 2 preventing and treating rice leaf roller biological insecticides, it is characterised in that：Aglaia odorata spend 20 ~ 25 parts,
15 ~ 20 parts of the fruit of Rangoon creeper, 8 ~ 12 parts of needle juniper, 8 ~ 12 parts of white pouring grass, 8 ~ 12 parts of cuckoo meat, 8 ~ 12 parts of Inula britannica chinensis, vernonia anthelmintica 6 ~ 8
Part, 6 ~ 8 parts of elm bark, 6 ~ 8 parts of the tuber of stemona, 6 ~ 8 parts of allicin, 2 ~ 3 parts of bacteria agent.
4. as claimed in claim 3 preventing and treating rice leaf roller biological insecticides, it is characterised in that：Original by following weight portion
Material is prepared from：Aglaia odorata spend 25 parts, 15 parts of the fruit of Rangoon creeper, 12 parts of needle juniper, white drench 8 parts of grass, 12 parts of cuckoo meat, 12 parts of Inula britannica chinensis,
8 parts of vernonia anthelmintica, 6 parts of elm bark, 6 parts of the tuber of stemona, 8 parts of allicin, 2 parts of bacteria agent.
5. a kind of preventing and treating rice leaf roller process for preparing bio-insecticide, it is characterised in that：Comprise the following steps：
1）The Aglaia odorata for weighing dry weight portion respectively spends 10 ~ 30 parts, in vain 10 ~ 30 parts of the fruit of Rangoon creeper, pouring careless 5 ~ 20 parts, cuckoo meat
3 ~ 12 parts of 5 ~ 20 parts, 3 ~ 12 parts of vernonia anthelmintica, 3 ~ 12 parts of elm bark and the tuber of stemona, mixing are carried out after freezing under nitrogen protection
Crush, obtain comminuting matter I；The needle juniper 5 ~ 20 part and Inula britannica chinensis 5 ~ 15 part of dry weight portion are weighed respectively, and mixing is crushed,
Obtain comminuting matter II；
2）The water of 4 ~ 6 times of its weight is added in the comminuting matter I, 40 ~ 50 DEG C are heated to, and is kept for 40 ~ 50 minutes, mistake while hot
Filter, obtains filtrate I and the dregs of a decoction I, filtrate is cooled down, sealing preserve；In the water that the dregs of a decoction I add 2 ~ 3 times of its weight, it is heated to
50 ~ 60 DEG C, kept for 30 ~ 40 minutes, filter while hot, obtain filtrate II and the dregs of a decoction II；The filtrate I and filtrate II are merged and is obtained
Filtrate, when the filtrate is concentrated into 75 DEG C, relative density is 1.12 ~ 1.15 medicinal extract, adds volume fraction in the medicinal extract
For 75 ~ 80% ethanol solution, 25 ~ 30 DEG C of temperature, kept for 18 ~ 24 hours, then filter, obtain filtrate III and sediment, by institute
State the lotion that relative density when filtrate III is evaporated to 55 DEG C is 1.20 ~ 1.25；
3）The water of its 3 ~ 5 times of weight of weight is added in the comminuting matter II, is added in distilling flask, is distilled, control
Distillation speed oozes the drop of solution 2 ~ 3 for backflow per second, and distillation time is 35 ~ 40 minutes, and distillation is extracted 2 ~ 3 times, and extract is closed
And extracted with organic solvent, sub-cloud solution, vacuum revolving instrument steams organic solvent, obtains final product extract；
4）The lotion and extract are separately dried, are crushed, merged, obtain active ingredient I；
5）The active ingredient I is mixed with 5 ~ 10 weight portion of allicin, 1 ~ 5 weight portion of bacteria agent, 2000 ~ 3000rpm is stirred
Mix 5 ~ 10 minutes, obtain final product.
6. as claimed in claim 5 preventing and treating rice leaf roller process for preparing bio-insecticide, it is characterised in that：The leaching
The w/v 1g of cream and the ethanol：10~20ml.
7. as claimed in claim 5 preventing and treating rice leaf roller process for preparing bio-insecticide, it is characterised in that：Described have
Machine solvent is dichloromethane.
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