KR100637329B1 - Microbial pesticides having fungicidal and insecticidal activity - Google Patents

Abstract

The present invention relates to a microbial agent having antifungal and insecticidal activity.

The microbial nutrient having an antifungal and insecticidal activity of the present invention is prepared by mixing an insecticidal active bacterium concentrate and an antifungal active bacterium concentrate, a mixture of an extender and a sunscreen agent, and then adding a plant nutrient.

In addition, the antifungal and insecticidal activity of the microbial control agent of the present invention, after mixing the insecticidal active bacterial concentrate and the antifungal active bacterial concentrate, after adding and mixing the mucopolysaccharide and the biomatrix, the plant having insecticidal activity Prepare by adding and mixing the extraction concentrated mixture.

According to the present invention, there is provided a microbial agent having excellent sterilization and insecticidal effect, excellent stability and environmental preservation ability, and excellent growth promoting effect of crops and a method of manufacturing the same.

Antifungal, insecticidal, control, Bacillus, microorganisms, nutrients,

Description

Microbial agent with antifungal and insecticidal activity {MICROBIAL PESTICIDES HAVING FUNGICIDAL AND INSECTICIDAL ACTIVITY}             

1 is a manufacturing process of the microbial nutrient having antifungal and insecticidal activity of the present invention

Figure 2 is a manufacturing process of the microbial control agent having antifungal and insecticidal activity of the present invention

Figure 3 is a manufacturing process of the plant extract concentrated mixture having pesticidal activity

Figure 4 is a state diagram according to the manufacturing form of the microbial complex during the manufacturing process of the composite microbial control agent having antifungal and insecticidal activity of the present invention.

Ⓐ: Antifungal active bacterium (AF ⁺ cell) and insecticidal active bacterium (IC ⁺ cell) are mixed with biogel and MPS or PHB.

Ⓑ encapsulates antifungal active bacteria (AF ⁺ cells) and insecticidal bacteria (IC ⁺ cells) with MPS or PHB and is encapsulated with biogel.

Ⓒ: Antifungal active bacterium (AF ⁺ cell) and insecticidal active bacterium (IC ⁺ cell) are coated with biogel and finally filmed with MPS or PHB.

Ⓓ: Antifungal active bacteria (AF ⁺ cell) and insecticidal active bacteria (IC ⁺ cell) coated with biogel, encapsulated with MPS, and encapsulated using PHB, PHA / PL.

Ⓔ: MPS is mixed with the antifungal active bacterium (AF ⁺ cell), insecticidal active bacterium (IC ⁺ cell) and nitrogen-fixed bacterial culture solution.

The present invention relates to a microbial agent having antifungal activity and insecticidal activity.

Biopesticides are the commercialization of pathogens, host resistant microorganisms, natural pesticides and natural enemies collected in the natural environment to control pests, pathogenic microorganisms and weeds of crops.

Biological pesticides are currently being reported worldwide, including 145 biopesticides, classified into microorganisms, biochemical pesticides including pheromones, plant pesticides, natural enemies and others (Biopesiticide Manual, 1998).

In June 2000, Korea announced the registration criteria and methods of microbial pesticides, and systematically supported the microbial pesticides among the soil microbial products, which are recognized for their bactericidal and insecticidal effects. The opportunity to enter.

These biopesticide products are used in the form of powders, alginate prills, etc., which then increase the effectiveness of the product by spreading the product with a filler as a filler or a mixture of electrodepositer. There was an attempt to let.

Recently, a method of immobilizing spores in a biopolymer such as alginic acid, polyacrylamide, and carrageenan, and then mixing with various additives (nutrition agent, electrodeposition UV blocking agent, enhancer, PGPR, etc.) and spraying on a package has been developed.

However, it can be seen that the conventional microbial pesticide is only a pioneer stage in consideration of the drug persistence, pesticidal activity range, etc. in the soil treatment field, and large success cases have not been reported in the foliar spray field.

Also, the most important thing in commercialization is the most essential requirement that can be commercialized only in the state of production, storage, and processing in consideration of the efficacy, commercial viability, economic feasibility, and the amount and price of additives advantageous for pest control. to be.

Therefore, in the development of biological control agents, it is possible to isolate useful microorganisms with excellent antagonism, secure efficient delivery system technology for microbial drugs, secure efficient mass production technology, increase productivity of active materials by introducing advanced genetic engineering technology, and apply outdoor packaging applications of biological products. More research and development is needed for the development of the lamp.

Korean Patent Publication No. 054108 (A novel Bacillus thuringiensis BP865 having both insecticidal and antifungal properties), Bacillus thuringiensis subspecies Kustakii (Bacillus) having both insecticidal and insecticidal growth inhibitory effects on insect pests and phytopathogenic fungi thuringiensis subsp.kurstaki) BP865 (KCTC 8689P) is disclosed.

In Korean Patent Publication No. 10-0245514 (streptomyces strain producing antifungal substances and pesticides and a biopesticide composition containing a culture medium thereof), strains of the genus Streptomyces producing antifungal substances, pesticides and chitin degradation inhibitors And it relates to a biopesticide, characterized in that the culture medium of the strain is bonded to dry starch, crude protein and rock powder.

However, the conventional invention as described above has a problem that the antifungal and insecticidal activity is lowered, the stability and environmental preservation ability is lowered, and the growth promoting effect of the crop is not great.

In order to solve the above object, an object of the present invention is to provide a microbial agent for comprehensive control and a method of producing the same, having excellent sterilization and insecticidal effects, excellent stability and environmental preservation ability, and excellent growth promoting effect of crops.

The present invention relates to a microbial agent having antifungal and insecticidal activity.

More specifically, the microbial agent having antifungal and insecticidal activity of the present invention relates to a microbial nutrient having antifungal and insecticidal activity and a microbial control agent having antifungal and insecticidal activity.

The microbial nutrient having an antifungal and insecticidal activity of the present invention is cultivated Bacillus thuringiensis strain showing the insecticidal activity, and then concentrated to prepare a bactericidal active bacterium concentrate, Bacillus subtilis showing antifungal activity ( Bacillus subtilis ), and then concentrated to produce antifungal active bacterial concentrate, and then prepared insecticidal active bacterial concentrate and the antifungal active bacterial concentrate prepared to prepare a bacterial concentrate mixture, and then to a bacterial concentrate mixture It is prepared by mixing a filler and a sunscreen, and a microbial nutrient is prepared by adding a plant nutrient to the microbial mixture.

The microbial control agent having an antifungal and insecticidal activity of the present invention is cultivated Bacillus thuringiensis strain showing the insecticidal activity, and then concentrated to produce an insecticidal bacterium concentrate, and Bacillus subtilis showing the antifungal activity ( Bacillus subtilis ) After culturing the strain, and concentrated to prepare antifungal active bacterial concentrate, and then prepared insecticidal active bacterial concentrate and antifungal active bacterial concentrate mixed to prepare a bacterial concentrate mixture, muco to the bacterial concentrate mixture A microsaccharide is prepared by adding and mixing a polysaccharide and a biomatrix, and a microbial control agent is prepared by adding and mixing a plant extract concentrated mixture having pesticidal activity to the microbial complex.

Insecticidal bacterium in the present invention uses Bacillus thuringiensis strain.

After culturing Bacillus thuringiensis strain, concentrated 10-fold by using a centrifuge to produce a bacterial concentrate, and at the time of cultivation by adjusting the carbon source, nitrogen source and phosphate source and adding amino acids and vitamins, Cultivate and adjust to maximize the pesticidal effect.

In the present invention, the antifungal bacterium uses Bacillus subtilis strain.

After culturing Bacillus subtilis strains, concentrated bacteria were concentrated 10 times using a centrifugal separator to prepare bacterial concentrates.In this case, the optimum culture conditions and insecticides of the strains were added by adding amino acids and vitamins while controlling the carbon source, nitrogen source and phosphate source. Cultivate and adjust to maximize the effect.

In the process of preparing the microbial nutrient of the present invention, the microbial mixture is prepared by mixing 10 to 20 wt% of the sunscreen agent with respect to the weight of the bacterium concentrate mixture, and 70 to 90 wt% of the bulking agent in the bacterial concentrate mixture prepared in the present invention. do. In this case, the electrodeposition agent may be optionally added, and when added, 10 wt% of the bacterial concentrate is added to prepare a microbial mixture.

The microbial nutrients prepared in this way are excellent in controlling plant diseases because they contain antifungal active bacteria as well as the growth effect of crops when sprayed on plants.

In addition, the pesticide-active plant extract concentrated mixture used in the preparation of the microbial control agent having antifungal activity and insecticidal activity of the present invention, after extracting alcohols of Ginseng, red ginseng, bran, and night, respectively, concentrated to prepare a concentrated extract, camellia, The hot water extract of Jinrang, Sagunja, white paper, and Yeo-ro, respectively, concentrated to prepare concentrated extracts, and then, the concentrated extracts prepared by mixing the prepared concentrated extracts in the same ratio are used.

The microbial nutritional agent and the microbial control agent of the present invention can be used in liquid and powder state, and are diluted to 250 to 1,200 times when used. When applied by the direct spray method, the insecticidal effect is large within 24 hours, the effect is also fast, and there is no harm to the natural insects and insects, so it is a comprehensive microbial control agent with environmental protection function.

On the other hand, the biomatrices used in the present invention is to maintain the supply of nutrients, protection of insecticidal active bacteria, increase germination rate, short-term and long-term growth, etc. in order for insecticidal bacteria to exhibit pesticidal activity while maintaining survival in a natural environmental ecosystem or plant. Used conventional biomatrices.

Korean Patent Publication No. 10-0325632 (microbial having antifungal activity against pathogenic fungi, an antifungal microbial pesticide containing the same and a method for preparing the same), and Korean Patent Application Laid-Open No. 2002-0090249 (antifungal) A novel microbial bacilli Bacillus subtilis IB 3 and a microbial pesticide containing the same) are disclosed in the biomatrix.

Microbial nutrients and microbial control agents having antifungal activity and insecticidal activity of the present invention are excellent in antifungal and pesticidal effects against fungi and pests that cause harm to the following plants and animals.

Ie, plant pathogenic fungi Pyricularia oryzae , Rhyzoctonia solani , Rotting disease ( Mucor spp.), Receiving disease ( Pythium ultimum ), Tomato rotani ( Alternaria solani ), Root disease ( Fusarium oxysporum ), Apple decay disease ( Bodeaoshaeria dothi ), Sesame seed disease ( Cochliobolus myabeanus ), ash fungus ( Botrytis cinerea ), pepper anthrax ( Colletotrichum gloeosporioides ), watermelon creeper blight ( Mycosphaerela melonis ), pepper blight ( Phytophthora capsici ), tomato blight ( Phytophthora red rust) ( Puccinia recondita f.sp. tritici ), Candida albicans , etc. have excellent antifungal activity.

Also, pests of crops, Brown PlantHopper (BPH), Two Spotted SpiderMite (TSSM), Tobacco Cutworm, TCW: Tobacco CutWorm, Blueworm, Oyster Fly, Aphid, Pear Tree, Green Beetle Moth, Deep Moth It has excellent insecticidal activity against insects, greenhouse dusts, slugs, aphids, rice weevils, pear moths, and moths.

The present invention shows an excellent effect on fungi and pests harmful to plants and animals as described above, and can be usefully used as a multifunctional environmentally-friendly microorganisms and pesticides with non-toxic environmental preservation and purification for natural enemies, bees, and human beings.

That is, the microorganism of the present invention may be used as an active ingredient such as soil improving agent, composting agent, foliar spraying agent or irrigation spraying agent, in addition to insecticidal and antifungal activity, having organic matter degrading ability, pesticide resistance and pesticide degrading ability.

The present invention is to determine whether the antifungal active bacteria of the present invention have an environmental purification ability, the results of the organic decomposition, pesticide resolution and pesticide resistance, etc., the antifungal active bacteria are all excellent in organic matter decomposition ability, chitin resolution and lipid degradation capacity It was confirmed to be strong.

In addition, the antifungal active bacterium is resistant to 21 kinds of pesticides including herbicides, fungicides and insecticides, and exhibits resistance to various types of pesticides since it degrades pesticides. Therefore, the antifungal active bacterium of the present invention can be used as an environmentally friendly multifunctional antifungal agent useful for environmental conservation and purification.

In addition, the antifungal active strains of the present invention did not affect the growth of the plant by measuring the seed germination rate, so that it did not inhibit the seed germination rate at all, which is useful for storage and preservation as well as microbial pesticides and seed encapsulation. Could confirm.

The microbial preparation for comprehensive control having antifungal and insecticidal activity of the present invention can be modified and manufactured in a conventional manner in the form of a raw agent, a hydrating agent, and a coating granule according to the use, and can be diluted to 400 to 1,200 times when used.

Hereinafter, the microbial agent for comprehensive control having antifungal and insecticidal activity of the present invention will be described in detail through Examples and Experimental Examples, but these are not intended to limit the scope of the present invention.

Example 1 Preparation of Bacterial Concentrate Having Insecticidal Activity

Two strains, KCTC 1508 and KCTC1509, were prepared from Bacillus thuringiensis strains showing insecticidal activity deposited at the Korea Biotechnology Research Institute Gene Bank (KCTC).

The strain was incubated in GPB medium (Table 1) at 30 ° C. for 72 hours and then concentrated 10-fold using a centrifuge to prepare a bacterial concentrate.

Table 1 GPB Medium Composition

Furtherance Content (g / ℓ) Remarks Glucose 20 Separately sterilized and mixed Peptone 5 (NH ₄ ) ₂ SO ₄ 4 K ₂ HPO ₄ One MgSO ₄ .7H ₂ O One MnSO ₄ .7H ₂ O 0.07 CaCl ₂ .2H ₂ 0 0.08 pH 7.0 with 2N NaOH, 1N HCl

Example 2 Preparation of Antifungal Active Bacterial Concentrate

The inventors of the present invention, Bacillus subtilis amylolytics (KCTC 8913P), Bacillus subtilis EBM 13 (KCTC 0984BP) exhibiting strong antifungal activity against pathogenic fungi deposited by the Institute of Biotechnology Research Institute of Korea Institute of Science and Technology Was used.

To the Glu-MOPS (pH 7.0) medium, 0.5 vol% of peptone, beef extract and tryptone as nitrogen sources, fructose, galactose and glycerol were respectively added as carbon sources, vitamin B1 and phosphoric acid were added, and arginine, histidine, Medium was prepared by adding isoleucine, methionine, proline, threonine, tryptophan, valine.

After inoculating the prepared microorganisms and incubated for 72 hours at 30 ℃, it was concentrated 10 times using a centrifuge to prepare a bacterial concentrate.

Example 3 Preparation of Plant Extract Concentrated Mixture Having Insecticidal Activity

On the market, they prepared Goren, Ginseng, Saekbaek, Noodles, Camellia, Betel, Sagunja, White Paper and Jeolla.

Cinnamon (root), red ginseng (root, stem), cypress (leaves, stem) and wild ginseng (root, stem) are ground and soaked in 2 kg of 75% Et-OH. Extraction was added with shaking for 4 hours.

After filtering the alcohol extract, and concentrated to 6 times while heating to 90 ℃ to prepare an extract concentrate 1, respectively.

Camellia (flowers, leaves), betel nuts (roots), sandals (roots), white paper (shells), and paths (roots) are not crushed, and 1 kg each of 10 l of water is heated and heated to 90 ° C for 24 hours. Extracted.

6 L of 75% Et-OH was added to 1 L of the hot water extract, heated to 90 ° C. for 6 hours, concentrated, and filtered to prepare extract concentrate 2.

The prepared extract concentrate 1 and the extract concentrate 2 were mixed in the same ratio, and then filtered by adjusting the volume with 30% Et-OH to prepare a plant extract concentrate mixture showing the final pesticidal activity.

Example 4 Preparation of Microbial Nutrient Having Antifungal and Insecticidal Activity

Insecticidal bacteria concentrate of Example 1 was prepared.

The antifungal active bacterial concentrate of Example 2 was prepared.

Commercial phytonutrients (containing nitrogen, phosphoric acid, potassium, amino acids, other minerals, etc.) were commercially available and prepared.

Insecticidal active bacterial concentrate was prepared by mixing the prepared insecticidal and bacterial concentrations in the same ratio.

500 g of the bacterial concentrate mixture was mixed with 415 g of a filler and 80 g of a UV blocker to prepare a microbial mixture.

To 100 g of this microbial mixture, 400 ml of the plant nutrient was prepared to prepare a microbial nutrient having antifungal and insecticidal activity.

Example 5 Preparation of Microbial Control Agent Having Antifungal and Insecticidal Activity

Insecticidal active bacterial concentrate of Example 1 and antifungal active bacterial concentrate of Example 2 were prepared.

Mucopolysaccharide (MPS), a biopolymer , was cultured at 30 ° C. under nitrogen nitrogen bacteria Rhizobium loti and Rhizobium meliloti to prepare a culture solution.

The polymer materials shown in Table 2 were mixed.

A biopolymer material in the mixture of poly-beta-hydroxy-alkanoates (PHB) for mixing and, Rhizobium loti, Rhizobium meliloti the above-mentioned number of microbial cells, 10 ⁸ to 10 ¹⁰ per gram polymer mixture as biopolymer synthesis microorganism is Mixing to make a biomatrix.

The biomatrices were sterilized and then cooled and prepared at 50 ° C.

A bacterium concentrate mixture was prepared by mixing the Bacillus thuringiensis bacterial concentrate of Example 1 and the Bacillus subtilis bacterial concentrate of Example 2 at the same ratio.

500 ml of the mucopolysaccharide (MPS) prepared in 500 ml of this bacterial concentrate and 200 g of the biomatrix were mixed and encapsulated.

At this time, the biomatrix was prepared so that the number of cells per gram of the polymer material 10 ⁸ ~ 10 ¹⁰ , and then mixed uniformly in a homogenizer (homogenizer) to make microorganisms evenly fixed in the membrane secondary microbial complex was prepared.

The microbial control agent having the antifungal and insecticidal activity of the present invention was prepared by mixing 0.4 l of the insecticidal active plant extract concentrated mixture with 100 g of the microbial complex.

<Table 2> Polymer Materials

ingredient Amount Soybean Flour + Seaweed Powder 400 g (280 + 120) glucose 2 g peptone 2 g MgCl ₂ 0.04 g CaCO3 2 g FeSO ₄ .7H ₂ 0 120 mg MnCl ₂ .4H ₂ O 20 mg KH ₂ PO ₄ 0.1 g Vermiculite 40 g H ₂ O 1 ℓ

Comparative Example 1 Comparative Preparation of Microbial Control Agents

To prepare a microbial control agent in the same manner as in Example 5, the microorganisms used were used only in Example antifungal active bacterial concentrate. That is, the pesticide active bacterial concentrate of Example 1 and the pesticide active plant extract concentrated mixture of Example 3 were prepared without the addition of a microbial control agent.

Experimental Example 1 Investigation of Safety and Toxicity of Antifungal and Insecticidal Microbial Control Agents

An antifungal and insecticidal active microbial control agent of Example 5 of the present invention was prepared.

The prepared microbial control agent was cultivated by applying to the lettuce culture packaging.

The cultivated lettuce was referred to the National Agricultural Products Quality Management Service for harmful pesticide composition and toxicity analysis, and the survey results are shown in Table 4 below. This result shows that there is no problem in applying the microbial agent of the present invention to organically grown farms.

<Table 4> Stability and Toxicity Survey Results

Black item Lettuce Sample score and weight 1 point / 2kg Inspection July 1, 2003 Inspection items Alachlor, Biphenox, Bifenthrin, Chinomethionate, Chlorophenapyr, Chlorotaronyl, Cyfluthrin, Cyhalothrin, Cypermethrin, Deltamethrin, Diclofluanide, Dicopol, Difenokazole , Endosulfan, espenvalrate, etoprofos, feminarimol, phenprophrin, fenvalrate, flucitrinate, flufenoxuron, fluvalinate, holepet, iprodione, isoprothiolan, meso Tribuzin, oxadione, oxyfluoroorpene, fenconazole, permethrin forrate, posalon, procmidone, propazite, prothiophosphate, pyrazophosphpyridabene, tetradipon, torplophosphamyl, traral Rometrin, triadimefon, trituralin, triflumizol, vinclozoline, bitteranol, buprofezin, chlorpyrifos, chlorpyrifosmethyl, diazinone, dimethoate, edifene, efien, phenytro Chion, Phenobuca , Phenyl, Pyrylazole, Hexaconazole, Iprobenps, Malathion, Metalaccil, Methc Dathione, Michaelrobutanyl, Parathion, Fendimethalin, Pentoate, Phosmet, Pyrimicarb, Pyrimiphos Methyl, propenophos, simazine, tebuconazole, tervus, chiobencarb, carbendazim, diflubenzuron, dimethomorph, imidacloprid, teflubenzuron, cabaril, cabofuran, mechiocarb, Mesomil (more than 82 ingredients) Test result Not detected: 82 components

Experimental Example 2 Analysis of Bactericidal Insecticidal Activity of Antifungal and Insecticidal Microbial Control Agents

An antifungal and insecticidal active microbial control agent of Example 5 of the present invention was prepared.

An antifungal active microbial control agent of Comparative Example 1 was prepared.

The bactericidal activity was tested by inoculation of each pathogen after 1 day of treatment.

The analysis results are shown in Tables 5 and 6 below.

<Table 5> Sterilization Effect

division Comparative Example 1 Example 5 Rice Fever (RCB) 69 79 Rice Leaf House Blight (RSB) 10 20 Tomato Gray Mold Disease (TGM) 31 44 Tomato Plague (TLB) 70 86 Wheat Red Rust (WLR) 43 90 Barley Powder (BPM) 25 25 Cucumber Gray Mold Disease (CGM) 83 - Cucumber powdery mildew (CPM) 87 -

Table 6 Insecticidal Effects

division Comparative Example 1 Example 5 BPH (BPH) 24 h 0 100 48 h 0 100 Chinese cabbage moth (DBM) 24 h 0 100 48 h 0 100 Spotted Ngae (TSSM) 24 h 0 100 48 h 0 100 Tobacco Seminar (TCW) 24 h 0 80 48 h 0 100 Whisker 24 h 0 100 48 h 0 100 Gullfly 24 h 0 100 48 h 0 100

* The number indicated indicates the control effect in%.

(100: 100% control rate, 0: no drug effect)

As shown in Table 5, the microbial water control agent having the antifungal and insecticidal activity of the present invention, when treated with the antifungal active microbial control agent of Comparative Example 1 of rice bleeding disease, tomato late blight, cucumber gray mold, powdery mildew Controls were 69%, 70%, 83% and 87%, respectively. This is almost the chemical pesticide control price.

However, when the microbial control agent having antifungal and insecticidal activity of the present invention was treated, it was found that sterilization control values of rice fever, tomato late blight, and wheat red rust were all enhanced.

In addition, as shown in Table 6 it can be seen that the pesticidal effect of the microbial control agent of the present invention was also excellent. That is, it can be seen that the microbial control agent of the present invention has a large insecticidal effect as well as a bactericidal effect.

Experimental Example 3 Greenhouse and Packaging Activity Tests of Microbial Nutrients with Antifungal and Insecticidal Activities

The microbial nutrient having antifungal and insecticidal activity of Example 4 of the present invention was prepared by diluting 250 times with tap water.

In order to control cucumber powdery mildew, Downy mildew, and Black Star disease, which occur naturally in cucumber greenhouses greenhouse farmhouse located in Sangju, Gyeongbuk, by diluting the antifungal active strain of the present invention, spraying twice a week directly on the leaf surface showing severe symptoms Antagonism was tested.

As a result, it showed the effect of delaying the development of lesions. Therefore, it was found that the microbial nutrient of the present invention has high antifungal activity.

Experimental Example 4 Effect Test of Microbial Nutrients with Antifungal and Insecticidal Activities

Microbial nutrients having antifungal and insecticidal activity of Example 4 of the present invention were prepared.

The crops were tomato (variety: dotarang), cucumber (variety: fresh green tea), red pepper (variety: green mine), cabbage (variety: yellow), and five mountain crops.

Experiments were divided into experimental, standard fertilization control, and no control.

Experimental Example 4 treatment group of the present invention was diluted 250 times and 500 times each in tap water and sprayed evenly on the surface of the crop by about 35 ml per week.

The standard fertilizer control was diluted once a week by diluting horticulture liquor Nargen 2000 (Crew) Co., Ltd. 1,000 times.

In the untreated area, nothing but water was treated.

1. Soil Survey Experiment

Soil electrical conductivity and soil acidity did not change significantly between treatments.

Soil pH was in the range of 5.17 to 5.84, with no significant differences between treatments.

Soil electrical conductivity (EC) was in the range of EC 28.4-33.7 실험 / cm in the experimental group, 30.5-32.7 ㎲ / cm in the untreated control, and 30.3-33.4 ㎲ / cm in the standard fertilizer.

That is, the microbial nutrient of the present invention did not appear to significantly change the acidity or electrical conductivity of the soil.

In addition, immediately after harvesting the cultivated crops, the soil was collected by treatment to examine the microbial density, showing a difference between the treated and untreated groups (Table 3).

The total bacterial density of the soil was less than 10 ³ cfu / cm ^{3 in the} untreated control and standard fertilization, but 10 ³ ~ 10 ⁵ cfu / cm ^{3 in} the microbial nutritional treatment of Example 4. Therefore, it can be said that bacteria, which are microorganisms of these microbial nutrients, also settle well in the soil.

2. Growth promotion effect test

All crops were harvested and examined within 35 days of planting, regardless of plant type and treatment.

By taking the above-ground relative to the surface of each plant were measured and plant height to choejeongah (shoot apical meristem) to 0.5 cm units, 0.1 g was units measure the fresh weight up, the leaf number and ipmyeonjeok in conventional manner 0.1 cm ² units Measured to the following Table 7 and 8.

<Table 7> Measurements of Ground Height and Raw Weight by Crops

division Example 4 Treatment Tool Standard Fertilizer No treatment 250-fold dilution 500 times dilution 1,000-fold dilution tomato Extra long (cm) 45.2 (171.9) a 39.6 (150.6) b 28.8 (109.5) de 26.3 (-) e Raw weight (g) 18.3 (181.1) a 12.1 (119.8) bc 10.6 (105.0) c 10.1 (-) c cucumber Extra long (cm) 141.0 (131.4) a 121.8 (113.5) b 109.2 (101.8) de 107.3 (-) e Raw weight (g) 35.8 (182.7) a 27.5 (140.3) b 22.3 (113.8) d 19.6 (-) d pepper Extra long (cm) 24.6 (190.7) a 17.7 (137.2) bcd 15.9 (123.3) bcd 12.9 (-) d Raw weight (g) 6.3 (150.0) a 5.2 (123.8) abcd 4.6 (109.5) cd 4.2 (-) d cabbage Extra long (cm) 21.6 (131.7) a 16.2 (98.8) cd 17.8 (108.5) bc 16.4 (-) cd Raw weight (g) 10.7 (175.4) a 6.4 (104.9) b 6.9 (113.1) b 6.1 (-) b Dolsangat Extra long (cm) 20.4 (114.6) abc 19.8 (111.2) abc 18.5 (103.9) abc 17.8 (-) bc Raw weight (g) 10.7 (142.7) ab 8.7 (116.0) bc 7.6 (101.3) c 7.5 (-) c

* Numbers in parentheses are percentages of untreated control

* The same alphabet within the same growth characteristics has no statistically significant difference in Duncan's multiple test (P = 0.05)

<Table 8> Results of Leaf and Leaf Area Measurement by Crops

division Example 4 Treatment Tool Standard Fertilizer No treatment 250-fold dilution 500 times dilution 1,000-fold dilution tomato Coniferous tree 8.7 (138.1) a 7.4 (117.5) bc 6.8 (107.9) cd 6.3 (-) d Leaf area (㎠) 415.3 (165.9) a 310.2 (123.9) b 274.9 (109.8) c 250.3 (-) c cucumber Coniferous tree 13.1 (172.4) a 12.8 (168.4) a 8.8 (115.8) c 7.6 (-) d Leaf area (㎠) 957.7 (229.9) a 853.6 (204.9) b 558.3 (134.0) d 416.6 (-) e pepper Coniferous tree 12.3 (186.4) a 10.1 (153.0) abc 10.2 (154.5) bcd 6.6 (-) e Leaf area (㎠) 369.7 (181.7) a 298.4 (146.6) abc 250.6 (123.1) abc 203.5 (-) c cabbage Coniferous tree 8.3 (112.2) a 8.1 (109.5) ab 8.1 (109.5) ab 7.4 (-) bc Leaf area (㎠) 274.3 (177.5) a 168.0 (108.7) bc 170.8 (110.6) bc 154.5 (-) c Dolsangat Coniferous tree 6.4 (116.4) ab 6.2 (112.7) ab 6.8 (123.6) a 5.5 (-) b Leaf area (㎠) 197.8 (150.9) ab 191.4 (146.0) ab 127.5 (97.3) b 131.1 (-) b

* Numbers in parentheses are percentages of untreated control

* The same alphabet within the same growth characteristics has no statistically significant difference in Duncan's multiple test (P = 0.05)

As shown in Tables 7 and 8 above, it was found that the most accelerated growth in all crops was 250 times the microbial nutritional treatment of the present invention.

That is, tomato showed 50 ~ 80% increase in height, fresh weight, and leaf area of 250 times the microbial nutritional treatment. Cucumber showed more than 80% fresh weight and 130% leaf area.

In addition, the pepper growth rate and leaf number growth rate of the 250 times the microbial nutritional supplements were higher than 80%, and the cabbage showed the above 70% growth of the ground weight and the leaf area of the 250 times the microbial nutritional treatments.

According to the present invention, a microbial nutritional agent microbial control agent excellent in antifungal activity and insecticidal activity is provided.

In addition, excellent stability and environmental preservation ability, and excellent microbial nutrient and microbial control agent for the growth and growth effect of the crop is provided.

In addition, there is provided a method for producing microbial nutrients and microbial control agents excellent in antifungal activity and insecticidal activity.

Claims (8)

In the method for producing a microbial nutrient, Bacillus thuringiensis strains showing insecticidal activity were cultured by adding amino acids and vitamins while controlling carbon source, nitrogen source and phosphate source, and then concentrated 10-fold to prepare pesticide-active bacterial concentrates, Bacillus subtilis strain showing antifungal activity was incubated with amino acids and vitamins while controlling carbon source, nitrogen source and phosphate source, and then concentrated 10-fold to prepare antifungal active bacterial concentrate. After mixing the prepared insecticidal active bacterial concentrate and the antifungal active bacterial concentrate in the same ratio to prepare a bacterial concentrate mixture, A microbial mixture is prepared by mixing a filler and a sunscreen with the bacterial concentrate mixture. Adding a plant nutrient to this microbial mixture to produce a microbial nutrient having antifungal and insecticidal activity, Method for preparing microbial nutrient having antifungal and insecticidal activity. The method of claim 1, In the preparation of the microbial mixture, the microbial mixture is characterized by mixing 10-20% by weight of the sunscreen agent in the bacterial concentration mixture with respect to the weight of the bacterial concentration mixture, 70-90% by weight of the extender, plant nutrients per 100 g of the microbial mixture It is characterized by the addition of 400 ml to prepare a micronutrient Method for preparing microbial nutrient having antifungal and insecticidal activity. Prepared by the method of claim 1, Microbial nutrient having antifungal and insecticidal activity. In the method for producing a microbial control agent, Bacillus thuringiensis strains showing insecticidal activity were cultured by adding amino acids and vitamins while controlling carbon source, nitrogen source and phosphate source, and then concentrated 10-fold to prepare pesticide-active bacterial concentrates, Bacillus subtilis strain showing antifungal activity was incubated with amino acids and vitamins while controlling carbon source, nitrogen source and phosphate source, and then concentrated 10-fold to prepare antifungal active bacterial concentrate. After mixing the prepared insecticidal active bacterial concentrate and the antifungal active bacterial concentrate in the same ratio to prepare a bacterial concentrate mixture, The microbial complex is prepared by adding and mixing mucopolysaccharide and biomatrix to the bacterial concentrate mixture, The microbial complex consists of adding a plant extract concentrated mixture having insecticidal activity and mixing the same to prepare a microbial control agent having antifungal and insecticidal activity. Method for producing a microbial control agent having antifungal and insecticidal activity. The method of claim 4, wherein In the preparation of the microbial complex, characterized in that it is prepared by mixing the bacterial concentration mixture in a ratio of 1 liter of mucopolysaccharide and 400 g of biomatrix per liter of bacterial concentration mixture, Method for producing a microbial control agent having antifungal and insecticidal activity. The method of claim 4, wherein The concentrated plant extracts with insecticidal activity are alcohol extracts of ginseng, red ginseng, branch vein, and wild gland, respectively, and then concentrated to prepare concentrated extracts, respectively. After each extract is prepared, characterized in that the concentrated extract mixture is prepared by mixing all the same ratio, respectively, to produce a concentrated plant extract mixture, Method for producing a microbial control agent having antifungal and insecticidal activity. Prepared by the method of any one of claims 4 to 6, Microbial control agent having antifungal and insecticidal activity. The antimicrobial and insecticidal activity of the microorganism control agent of claim 7, Pyricularia oryzae , Rhizoctonia solani , Mucor spp., Pythium ultimum , Tomato Alternaria solani), Root rot (Fusarium oxysporum), apples rot (Botryoshaeria dothidea), kkaessi blotch (Cochliobolus myabeanus), jaetbit mildew (Botrytis cinerea), red pepper anthracnose (Colletotrichum gloeosporioides), watermelon blight (Mycosphaerela melonis), pepper blight ( Phytophthora capsici ), tomato late blight ( Phytophthora infestans ), wheat red rust ( Puccinia recondita f.sp. tritici ), candidiasis ( Candida albicans ), rice plant (BPH: Brown PlantHopper), spotted mite (TSSM: Two Spotted Spider Mite), tobacco Giant moth (TCW: Tobacco CutWorm), worm, oyster fly, aphid, pear tree, green leaf moth, carnivorous moth, whiskers, greenhouse dust, slug, aphid, rice weevil, pear How to control the one or more pests and fungal species selected one.

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