KR101465096B1 - Composition comprising the extract from the larvae of Hermetia illucens for controlling pathogenic microorganisms in plants - Google Patents
Composition comprising the extract from the larvae of Hermetia illucens for controlling pathogenic microorganisms in plants Download PDFInfo
- Publication number
- KR101465096B1 KR101465096B1 KR1020120112343A KR20120112343A KR101465096B1 KR 101465096 B1 KR101465096 B1 KR 101465096B1 KR 1020120112343 A KR1020120112343 A KR 1020120112343A KR 20120112343 A KR20120112343 A KR 20120112343A KR 101465096 B1 KR101465096 B1 KR 101465096B1
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- present
- composition
- kacc
- larvae
- Prior art date
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a composition for controlling plant pathogens including a larva extract of Hermetia illucens , and more particularly to a plant pathogen control and feed additive composition containing larvae extract as an active ingredient.
Since the larvae extract of the present invention exhibits an antimicrobial activity against plant pathogens, it is effective for the production of a composition for controlling plant pathogens and a fertilizer additive composition containing the larvae extract as an effective ingredient.
Description
The present invention relates to a composition for controlling plant pathogens including a larva extract of Hermetia illucens , and more particularly to a plant pathogen control and feed additive composition comprising a larva extract as an active ingredient.
Hermetia illucens has an elongated shape with a length of 12-20 mm and is generally black, with a large translucent pattern of white or yellowish brown on the second round. On the chest back plate, a light yellowish white hair bundle forms a vertical stripe and the average is white. The wings are transparent but black, with a slight violet shine and a darker front, which may appear to be yellowish brown depending on the angle. The more detailed name is also called 'the hominid of America', and ' Hermetia illucens' in addition to scientific name 'black soldier fly' is also called. It takes about 30 ~ 40 days from egg to adult, and adult survives about 10 days. It is used to improve environmental pollution caused by organic matter because it consumes various organic materials such as food waste and livestock manure.
In the agricultural cultivation of cash crops such as vegetables, it is often difficult to achieve high-quality stable profit of the product by occurrence of seed infectivity, soil infectivity, air (purchase) infectious plant disease. Especially when the disease is likely to cause disease, the production farmer suffers a catastrophic failure. It is indispensable to control plant diseases caused by these hospitals in order that normal cultivation can be carried out, which are mainly filamentous fungi (bacteria), bacteria (bacteria) and viruses, which are recognized as the cause of such infectious diseases.
Background Art [0002] It is a chemical method of administering pesticides (for example, fungicides, bactericides, and antiviral agents), which is widely practiced as an effective method for controlling plants from diseased plants. In recent years, organic synthetic pesticides have been widely spread due to their effects, and they have contributed greatly to improvement of crop production by exerting power in controlling diseases.
However, pest control against pesticide use is problematic in terms of global environmental, social, and agricultural problems such as soil residue of pesticides, food residue, and resistance of drug resistance to pathogenic microorganisms. In addition, excessive use of pesticides is also problematic in terms of safety of agricultural workers.
Accordingly, the present inventor has found that a larva extract of Hermetia illucens has antimicrobial activity against a plant pathogen while searching for a natural substance having antimicrobial activity against a pathogenic microorganism of a crop, thus completing the present invention.
It is therefore an object of the present invention (like Hermetia dongae The present invention also provides a composition for inhibiting plant pathogens, which comprises a larva extract as an active ingredient.
Another object of the present invention is to provide a feed additive composition for controlling plant pathogens, which comprises a larva extract of Hermetia illucens as an active ingredient.
In order to accomplish the object of the present invention, the present invention provides a composition for controlling plant pathogens, which comprises a larva extract of Hermetia illucens as an active ingredient.
In order to accomplish another object of the present invention, the present invention provides a fertilizer additive composition for controlling plant pathogens, comprising a larva extract of Hermetia illucens as an active ingredient.
Hereinafter, the present invention will be described in detail.
To examine the antibacterial activity of the larvae extract of Hermetia illucens , Pseudomonas sp. marginalis pv . marginalis , KACC 10466), red pepper bacillus ( Xanthomonas campestris pv . vesicatoria , KACC 11153 ), bacterial leaf blight ( Pseudomonas viridiflava , KACC 10391), Pseudomonas sp, (KACC 11132), tobacco wildfire pathogen (Pseudomonas syringae pv tabaci, KACC 12130 .), pepper bacterial jeommunuibyeong bacteria (Xanthomonas campestris pv vesicatoria KACC 12965. .), foot blight bacterium (Pseudomonas solanacearum - homogeneous two people:. Ralstonia solanacearum , RAC (radial diffusion assay) was performed on Xanthomonas axonopodis pv. Glycines (KACC 11151), KACC 10701, and KACC 10701) . As a result, antibacterial activities were shown in all bacteria (Examples <2-2> and (See Fig. 1).
The changes in the antimicrobial activity and the minimum inhibitory concentration (MIC) with time were analyzed by the method of P. viridiflava (KACC 10391), P. syringae pv. Tabaci (KACC 12130), P. marginalis pv. marginalis , KACC 10466) (see Examples <2-3> and <2-4>). In FIG. 2, the absorbance value of the control group rapidly increased with time, but it can be confirmed that the test group containing the larvae extract of the present invention showed no significant increase over time. In addition, in Table 1, the antibacterial activity was observed at all concentrations in Staphylococcus aureus (KACC 10466), 100 mg / ml of bacterial leaf blight bacterium (KACC 10391), 150 mg / ml of tobacco bacterium (KACC 12130) It can be confirmed that it exhibits antibacterial activity.
Accordingly, the present invention provides a composition for controlling plant pathogens, comprising a larva extract as an active ingredient in Donghae and the like.
The larvae extract of the present invention has a controlling effect against Pseudomonas sp. Or Xanthomonas sp. Pathogens.
More specifically, the Pseudomonas sp. Pathogen is selected from the group consisting of Pseudomonas marginalis pv. Marginalis , Pseudomonas viridiflava , Pseudomonas syringae pv. Tabaci , and Pseudomonas solanacearum . The present invention is characterized in that the pathogenic fungi belonging to the genus Zanthomonas are Xanthomonas campestris pv. Vesicatoria or Xanthomonas axonopodis pv. Glycines .
In addition, the larva extract of the present invention is characterized by being an organic solvent extract having 1 to 6 carbon atoms. The organic solvent having 1 to 6 carbon atoms includes acetic acid, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, Benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane. In the present invention, it is preferable that the organic solvent is selected from the group consisting of benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane. Most preferably acetic acid. The acetic acid may be in a concentration of 0.001% to 0.1% with respect to water.
The composition for controlling plant pathogenic bacteria according to the present invention can be formulated by mixing with agriculturally acceptable carrier materials or diluents. Such a composition may also be used as a fertilizer component or as a surfactant or a known plant disease controlling agent as an optional additional ingredient. The term "diluent " in the present invention means an agriculturally acceptable liquid or solid which is added to the larvae extract of the present invention for the convenience of use or used to dilute the larvae extract at the desired active concentration For example talc, kaolin, zeolite, xylene, diatomaceous earth, water and the like.
Formulations for use in spray form, such as water-dispersed concentrates or wet powders, may further comprise a wetting agent, a dispersant, a surfactant, and the like. In addition to the above-mentioned diluents and surfactants, stabilizers, deactivators, adhesion improvers, colorants, penetrating agents, antifoaming agents and the like may be further included.
The composition of the present invention may be formulated into various forms, and wettable powders prepared with kaolin, diatomaceous earth, etc. may be diluted with water and spray applied to leaves or applied to roots before being used as a spray solution. It may also be mixed with an emulsifying agent to obtain a concentrate, which may be diluted with water before application to a plant, or may be prepared, packaged and transported by a powder or granule such as lyophilization, spray drying or rotary drying, It can also be applied to plants. Application methods can be sprayed on leaves, stems or roots, sprayed on soil, or applied to soil by granulation or encapsulation.
The present invention also provides a fertilizer additive composition for controlling plant pathogens, comprising a larva extract as an effective ingredient of the present invention.
The fertilizer additive composition of the present invention can be added to a conventional fertilizer (for example, a fertilizer containing nitric acid, phosphoric acid, or potassium) or a mixture with a bactericide, a pesticide, a herbicide, a plant growth regulator, It is used in formulation.
The fertilizer additive composition of the present invention exhibits an antimicrobial activity against a pathogen occurring in a crop, thereby preventing a crop from being infected with a disease.
The content of the fertilizer additive composition of the present invention to be added to the fertilizer is not limited thereto, but is preferably 0.001 to 20 wt%, more preferably 0.01 to 10 wt% with respect to the total weight of the total fertilizer.
Since the larvae extract of the present invention exhibits an antimicrobial activity against plant pathogens, it is effective for the production of a composition for controlling plant pathogens and a fertilizer additive composition containing the larvae extract as an effective ingredient.
Of the pathogens used in the experiment - the diameter (mm), X-axis of the stop ring (Inhibition zone) - 1 is a result of measuring the radial diffusion assay (RDA) the antimicrobial activity of larval extract or the like dongae of the present invention (Y-axis. KACC NO.)
FIG. 2 (A) shows the result of the antimicrobial activity test for KACC 10391 ( P. viridiflava ) of the larvae extract of the present invention over time. ( Y axis - Absorbance, X axis - Hour (Hour))
FIG. 2B shows the result of the antimicrobial activity test for KACC 12130 ( P. syringae pv. Tabaci) of the larvae extract of the present invention over time. ( Y axis - Absorbance, X axis - Hour (Hour))
FIG . 2C shows the result of the antimicrobial activity test for KACC 10466 ( P. marginalis pv. Marginalis ) of the larvae extract of the present invention over time. ( Y axis - Absorbance, X axis - Hour (Hour))
Hereinafter, the present invention will be described in detail.
However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
≪ Example 1 >
Production of larvae extract from Dongae
Larvae were washed three times in sterilized water for 10 ~ 15 days and then lyophilized. The larvae were pulverized into lyophilized Donghae, and mixed with 0.01% acetic acid at a ratio of 10% (w / v). The mixture was subjected to a mixing process for 4 to 12 hours and purified using a 0.45-μm membrane filter. The extracts were once lyophilized and stored at -80 ° C until use.
< Example 2>
Antimicrobial activity measurement of larvae extract from Dongae
<2-1> Preparation and Culture of Experimental Strain
Pseudomonas spp . , KACC 10466), Pseudomonas spp.,
The cultivated plant pathogenic bacterium was subcultured on Trypton soya broth agar, Luria broth agar, and Nutrient agar medium, and then single colony was taken in a solid medium and a liquid medium.
<2-2> Radial diffusion analysis method RDA , radial diffusion assay )through Dong Ae Verification of the antimicrobial activity of larvae extract
All the strains of the above Example <2-1> were used for the radial diffusion analysis.
The strains were mixed with sterilized underlay gel for RDA at a concentration of about 10 < 6 > CFU / ml and solidified in a 100 mm square plate. At this time, a larva extract (250 mg / ml) was poured into a homogenate of Example 1 with a hole having a diameter of 5 mm, and the extract was incubated at 29 캜 for 3 hours to diffuse into the gel. After culturing, overlay gel for RDA was poured and hardened, followed by overnight incubation at 29 ° C. The antifungal activity of each peptide can be confirmed by the size of the inhibition zone appearing in the gel.
A photograph of the above test results and a graph of the size of the low-ring-opening diameter are shown in Fig. It can be confirmed that a low-kink is formed for all the strains tested in [Fig. 1]. Especially, the antimicrobial activity is active in the stem germ ( P. marginalis pv. Marginalis , KACC 10466). On the other hand, 0.01% acetic acid, which did not contain the larvae extract, was not found in the control group.
<2-3> Verification of antimicrobial activity over time
The antimicrobial activity of the larvae extract of the present invention over time was examined. Among the strains listed in Example <2-1>, P. viridiflava (KACC 10391), P. syringae pv. Tabaci,
20 ml of the above strain was added to 20 ml of the liquid medium, and then 1 ml (250 ml) of the larvae extract was applied to Dongae and the like. The treated medium was incubated at 29 DEG C with shaking, and the degree of growth was checked every 2 hours.
The absorbance measurement results are shown in Fig. In FIG. 2, the number of strains increased exponentially in the control group, while the number of strains in the experimental group containing the extract of the present invention was significantly lower than that of the control (0.01% acetic acid) after 16 hours have.
<2-4> Minimum inhibitory concentration ( minimal inhibitory concentration , MIC ) Measure
MIC means the minimum concentration required to inhibit the growth of the strain. Among the strains listed in the above Example <2-1>, P. viridiflava (KACC 10391), P. syringae pv . tabaci , KACC 12130), Staphylococcus aureus ( P. marginalis pv . marginalis , KACC 10466) were used.
Each of the above strains was adjusted to a concentration of 10 6 CFU / ml, and the extract of the present invention diluted to 50, 100, 150, 200, 250 ug / ml in an agar well having a diameter of 5 mm was prepared at various concentrations, And cultured at 29 DEG C for 48 hours. The MIC was determined by measuring the diameter of the hamstrings. As a control group, 0.01% acetic acid which did not contain larvae extract was used.
The measurement results are shown in Table 1 below.
strain
Since the larvae extract of the present invention exhibits antibacterial activity against plant pathogens, it can be used for the production of a composition for controlling plant pathogens and a fertilizer additive composition containing larvae extract as an effective ingredient, .
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020120112343A KR101465096B1 (en) | 2012-10-10 | 2012-10-10 | Composition comprising the extract from the larvae of Hermetia illucens for controlling pathogenic microorganisms in plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020120112343A KR101465096B1 (en) | 2012-10-10 | 2012-10-10 | Composition comprising the extract from the larvae of Hermetia illucens for controlling pathogenic microorganisms in plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20140046193A KR20140046193A (en) | 2014-04-18 |
| KR101465096B1 true KR101465096B1 (en) | 2014-11-25 |
Family
ID=50653239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020120112343A KR101465096B1 (en) | 2012-10-10 | 2012-10-10 | Composition comprising the extract from the larvae of Hermetia illucens for controlling pathogenic microorganisms in plants |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR101465096B1 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3026746B1 (en) | 2014-10-03 | 2021-09-10 | Pierre Furtos | PROCESS FOR THE PRODUCTION OF TARGET ANTIBIOTICS FROM INSECTS |
| KR101876939B1 (en) * | 2015-11-10 | 2018-07-11 | 전남대학교산학협력단 | Composition for controlling plant disease comprising Aspergillus persii strain producing penicillic acid as effective component and uses thereof |
| KR101961098B1 (en) * | 2016-09-28 | 2019-03-25 | 동국대학교 경주캠퍼스 산학협력단 | Method of manufacturing Hermetia illucens larvae to produce antibacterial substance and Antibiotic Compostion comprising the larvae |
| KR102114996B1 (en) * | 2017-08-31 | 2020-06-05 | 동국대학교 경주캠퍼스 산학협력단 | An Antimicrobial Composition comprising an Extract of an Hermetia illucens |
| CN107549126A (en) * | 2017-10-27 | 2018-01-09 | 银川保绿特生物技术有限公司 | Method for breeding black soldier fly larvae by using kitchen residual food base material |
| KR102181470B1 (en) * | 2019-02-21 | 2020-11-24 | 청주대학교 산학협력단 | Powder of the black soldier fly larva bred using dropwort feed containing germanium and composition for anti-oxidant and anti-cancer comprising the same |
| KR102303449B1 (en) * | 2020-04-16 | 2021-09-23 | 주식회사 파미니티 | A composition for promoting growth of ginseng and enhancing resistance to damages by blight and harmful insects, and a method for cultivating ginseng using the same |
| KR102316468B1 (en) * | 2020-04-16 | 2021-10-22 | 주식회사 파미니티 | A composition for promoting growth of ginseng and enhancing resistance to damages by blight and harmful insects, and a method for cultivating ginseng using the same |
| KR102260276B1 (en) * | 2021-01-19 | 2021-06-03 | (주)서일특수목재 | Wood preservation composition |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100557327B1 (en) | 2004-11-08 | 2006-03-06 | (주)바이오드림스 | Composition for controlling pathogenic microorganisms in plants |
-
2012
- 2012-10-10 KR KR1020120112343A patent/KR101465096B1/en active IP Right Grant
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100557327B1 (en) | 2004-11-08 | 2006-03-06 | (주)바이오드림스 | Composition for controlling pathogenic microorganisms in plants |
Non-Patent Citations (2)
| Title |
|---|
| Entomological Research 42 (2012) 219-226 * |
| ESA ANNUAL MEETINGS,2011 * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20140046193A (en) | 2014-04-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101465096B1 (en) | Composition comprising the extract from the larvae of Hermetia illucens for controlling pathogenic microorganisms in plants | |
| Harish et al. | Use of plant extracts and biocontrol agents for the management of brown spot disease in rice | |
| KR101715431B1 (en) | Plant pathogen inhibitor combinations and methods of use | |
| Kharwar et al. | Diversity and antimicrobial activity of endophytic fungal community isolated from medicinal plant Cinnamomum camphora | |
| US11723360B2 (en) | Application of methylmalonic acid in the preparation of nematode insecticides | |
| CN109890210A (en) | For treating the composition and method of citrus greening disease | |
| KR101695918B1 (en) | Streptomyces badius SP6C4 strain having antimicirobial activity against insect pathogen or strawberry fungal disease pathogen isolated from strawberry pollen and uses thereof | |
| CN105831175A (en) | Liquid compound biological multi-control fungicide and preparation method and application thereof | |
| KR20140071145A (en) | Novel Paenibacillus polymyxa AB-15 strain and use the same | |
| KR20120068674A (en) | Streptomyces griseus big105 for controlling plant diseases and uses thereof | |
| CN103503930A (en) | Botanical pesticide composition and application thereof | |
| KR101695916B1 (en) | Streptomyces badius SF7B6 strain having antimicrobial activity against pathogen for plant fungal disease isolated from flower of strawberry and uses thereof | |
| KR20160000537A (en) | New microorganism Beauveria bassiana FG274 and Microbial control agent for the prevention of Spodoptera exigua larva | |
| Saju et al. | In vitro evaluation of biocontrol agents, botanicals and fungicides against Pestalotiopsis sp. infecting large cardamom (Amomum subulatum Roxb.) | |
| KR101859926B1 (en) | Composition for controlling bacterial canker containing Actinidia arguta extract or its fraction as effective component and uses thereof | |
| KR20200057643A (en) | Novel Beauveria bassiana KNU-101 Strain with Improved Insecticidal Effect and Spore Production and Uses thereof | |
| KR20150113255A (en) | Beauveria bassiana having insect pathogenicityand agent for prevention of rice vermin using the same | |
| KR101795781B1 (en) | Mixed strain having nematicidal effect against root knot nematode and uses thereof | |
| CN106614642B (en) | Seed treatment agent composition and application thereof | |
| CN102484994B (en) | Synergy composition of matrine and chlorantraniliprole | |
| Narasimhan et al. | Efficacy of new EC formulations of neem oil and pungam oil for the management of sheath rot disease of rice | |
| KR101245582B1 (en) | Composition for controlling of rice blast containing aralia continentalis extract and a method of controlling rice blast using the composition | |
| CN113475529B (en) | Nematode preparation for preventing and treating flower slugs and preparation method and application thereof | |
| KR101148789B1 (en) | Microorganism for preventing Sclerotinia rot and uses thereof | |
| KR100976060B1 (en) | The novel antifungal bacterial paenibacillus polymyxa kc028 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant |