KR101634857B1 - Cultivation method of shiitake mushrooms - Google Patents

Abstract

The present invention relates to a method of cultivating a mushroom using a mushroom seed, and more particularly, it relates to a method for cultivating a mushroom of a mushroom cultivated in a first stage and a second stage, The present invention relates to a cultivation method of shiitake mushroom which can supply nutrients contained in a vinegar solution to a growth medium to provide vitality and antiseptic action by a vinegar solution.

Description

{Cultivation method of shiitake mushrooms}

The present invention relates to a method of cultivating a mushroom using a mushroom seed, and more particularly, it relates to a method for cultivating a mushroom of a mushroom cultivated in a first stage and a second stage, The present invention relates to a cultivation method of shiitake mushroom which can supply nutrients contained in a vinegar solution to a growth medium to provide vitality and antiseptic action by a vinegar solution.

Mushrooms are high protein foods and are expected to be a substitute for meat in the future. Especially, according to various recent research reports, glucan and its derivatives contained in mushrooms are known to have anticancer activities.

Mushrooms (basidiomycetes) are cultivated and cultivated under different conditions, but mushrooms are usually cultivated by inoculating the mushroom seedlings into a wood or mushroom culture medium.

The mushroom spawn is a seed for mushroom cultivation. It is called a seed culture including cultured cells and a medium. It is divided into a solid form and a liquid form according to the material and the form of a commonly used culture medium. Seed bacterium, sawdust bacterium and the like, and there is a liquid bacterium in liquid form.

When sawdust seeds are cultivated in oyster mushroom, shiitake mushroom, gentian mushroom, mulberry mushroom, etc., liquid seeds are used for cultivation of oyster mushrooms and various seeds.

Since such mushroom seeds correspond to mushroom seeds, the production and use of seeds of the seeds is a very important factor in determining the success of mushroom cultivation. The production of sawdust as solid seeds was carried out by mixing the sawdust, rice bran (rice bran) and other additives at a certain ratio in a medium of 60-65% moisture content into a PP or PE bottle. In a high pressure sterilizer, After sterilization for 60 minutes, it is cooled to room temperature of 25 ° C, seed germs of 5 ~ 10g per bottle are added and cultured in a culture room for 20-25 days to produce sawdust.

On the other hand, the Korean Registered Application No. 20-0397184 discloses that one end is sharpened and has a length (L) of 50-150 mm and a diameter (D) of 5-15 mm. Which is used for cultivating mushrooms.

However, the above-mentioned needles for the registration room are not described in the technique of increasing the harvesting cycle of the medium or increasing the yield because only the contents to be used only when the seedling is inoculated after the medium is sealed in the container is described .

SUMMARY OF THE INVENTION Accordingly, the present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a method for cultivating shiitake mushroom seedlings at a first stage and a second stage, The present invention also provides a method for cultivating shiitake mushroom using a seedling seedling.

It is another object of the present invention to provide a method for cultivating a mushroom using a needle-seed culture that can maximize the culture efficiency because the needle and the inoculation source cultivated in the primary culture are put together in a container for secondary culture.

It is also an object of the present invention to provide a method for producing a woody plant which is capable of providing a nutrient contained in a vinegar liquid to a growth medium and immersing it in a vinegar solution, And to provide a method for cultivating shiitake mushrooms.

It is also an object of the present invention to provide a method of cultivating a mushroom using a mushroom seed which can prevent the yield from decreasing even if the culture and harvest are repeated a plurality of times because the nutrition needle is additionally inserted into the growth medium.

In order to accomplish the above object, the present invention provides a method of cultivating a mushroom using a seed culture, comprising the steps of: preparing a bar needle immersed in bar in a nutrient solution; A step S2 of providing an inoculation source in which a seed culture is inoculated into a liquid medium or a solid medium and cultured in a primary culture; Step S3 of putting the needle into the container and sterilizing it; (S4) a step of culturing the inoculated source in the sterilized container by secondary culture, thereby producing a needled seed obtained by inoculating and culturing the seed; And a step S5 of inserting the seed culture into a growth medium for tertiary culture.

In the method for cultivating shiitake mushroom according to the present invention, the tree is immersed in the nutrient solution for 12 hours to 2 days, and the nutrient solution is diluted to 1 / 250-1 / 150 weight ratio of wood vinegar and water .

In the step S2 of culturing the shiitake mushroom using the seed germ according to the present invention, the seed is firstly cultured for 10 to 20 days, and the seed culture is secondarily cultured for 20 to 30 days in the step S4. do.

In addition, the growth medium of the method of cultivating shiitake mushroom according to the present invention is inserted into an encapsulation member to have a rectangular tubular shape, and a plurality of the seed propagules are inserted at regular intervals.

In the method of cultivating mushroom using the seed-seed culture according to the present invention, the seed cultured in the method is cultivated, and a band member is attached to the hole formed in the bag member so as to control the ventilation, A vent portion made of any one of cotton and sponge, and an adhesive portion formed on one surface of the vent portion and detachably fixed to the sealing member.

In step S5 of the method for cultivating shiitake mushroom according to the present invention, when the mushroom bacterium is deposited on the growth medium, the seed bacterium is removed, and in the state where the nutrient needle is additionally inserted into the removed site, The nutritional needle is characterized in that the needle is put into boiled nutrient solution diluted with wood vinegar and boiled for 30 to 60 minutes.

In the method for cultivating shiitake mushroom according to the present invention, grooves formed in a long length from the upper side to the lower side are formed. In step S5, when the mushroom bacteria are deposited on the growth medium, Or a third culture is performed in a state in which the nutrients are injected into the grooves, wherein the nutrient needle is put in a boiled nutrient solution diluted with wood vinegar and boiled for 30 to 60 minutes.

According to the method of cultivating shiitake mushroom using the seed-seed culture according to the present invention having the above-described constitution, since the shiitake mushroom seed culture is preliminarily cultivated in the first and second stages, it is possible to rapidly activate the growth medium and significantly reduce the culture period .

In addition, according to the method of cultivating shiitake mushroom using the seed germ seed according to the present invention, since the needles and the inoculation source in which the seed germ is firstly cultivated are put together in a container, the cultivation efficiency can be maximized.

According to the method of cultivating shiitake mushroom using the seedling seeds according to the present invention, since the bar-shaped wood is immersed in the vinegar solution, nutritional components contained in the vinegar solution are supplied, thereby providing vitality to the growth medium, There is an effect that antiseptic action can be expected.

In addition, according to the method of cultivating shiitake mushroom using the seed germ seed according to the present invention, since the nutrient gum needle is additionally inserted into the growth medium, the yield can be prevented from decreasing even if culturing and harvesting are repeated a plurality of times.

1 is a block diagram showing an embodiment of a method for cultivating shiitake mushroom using a bacterium according to the present invention.
2 is a perspective view showing a step of step S1 according to the present invention.
FIG. 3 is a view showing a state in which a seed culture is inoculated into a culture medium and subjected to primary culture in step S2 according to the present invention. FIG.
FIG. 4 is a view showing step S3 according to the present invention.
5 is a diagram showing step S4 according to the present invention.
6 is a perspective view and a cross-sectional view showing a state in which a seed culture is inserted into a growth medium according to the present invention.
FIG. 7 is a cross-sectional view showing a state in which a nutrition needle is inserted into a growth medium in step S5 according to the present invention. FIG.
FIG. 8 is a cross-sectional view showing a state in which a groove is formed in a necklace according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. In addition, the terms described below are defined in consideration of the functions of the present invention, and these may vary depending on the intention of the user, the operator, or the precedent. Therefore, the definition should be based on the contents throughout this specification.

1 is a block diagram showing an embodiment of a method for cultivating shiitake mushroom using a bacterium according to the present invention.

Referring to FIG. 1, the method of cultivating shiitake mushroom using the seed germ seed according to the present invention can be largely divided into steps S1 to S5.

Specifically, the method for cultivating shiitake mushroom using the seed germ according to the present invention comprises the steps of preparing a grommet, a step S2 of preparing an inoculation source in which a seed culture is first inoculated into a liquid medium or a solid medium, A step S3 of sterilizing the sterilized seedlings, a step S4 in which the seedlings are inoculated into the sterilized container to carry out secondary cultivation, thereby producing a seedling seedling inoculated and cultured in the seedling needle; and And S5 step of tertiary cultivation.

2 is a perspective view showing a step of step S1 according to the present invention.

Referring to FIG. 2, the step of step S1 according to the present invention can be exemplified by immersing a bar-shaped tree in a nutrient solution for 12 hours to 2 days.

The wood may be, but not limited to, oak.

When the height of the growth medium is 8 cm, the tree may have a diameter of 2 to 2 cm and a length of 5 to 7 cm, and may be variously formed depending on the size of the medium and the position of insertion. In addition, the tree can be used with a sharp end so that it can be easily inserted into a hardened medium.

The nutrient solution may be exemplified by a diluted wood vinegar solution in water.

The vinegar liquid is characterized not only by containing nutritional components necessary for mushroom growth but also by controlling the propagation of various bacteria due to its antibacterial or sterilizing effect.

The nutrient solution preferably has a weight ratio of wood vinegar to water of 1/25 to 1/150.

If the weight ratio of the vinegar solution to water is less than 1/250, the effect due to the vinegar solution can not be sufficiently exhibited. If the weight ratio is larger than 1/150, the yield of the mushroom is inhibited and the yield is reduced.

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FIG. 3 is a view showing a state in which a seed culture is inoculated into a culture medium and subjected to primary culture in step S2 according to the present invention. FIG.

Referring to FIG. 3, step S2 of the present invention provides an inoculation source in which a seed culture is inoculated to a solid medium containing water as a main component or a solid medium containing sawdust as a main component.

In addition, it is preferable that the step S2) is performed by primary culturing the seedlings for 10 to 20 days.

FIG. 4 is a view showing step S3 according to the present invention.

Referring to FIG. 4, step S3 according to the present invention is a sterilization method in which a needles are put into a container, which can be sterilized by high pressure sterilization and sterilization.

In the case of pasteurization, sterilization may be performed for 60 to 90 minutes in a sterilizer maintained at 121 占 폚.

The container may be a bottle or a plastic bag, and it is preferable that a lid having a ventilating structure is formed.

5 is a diagram showing step S4 according to the present invention.

Referring to FIG. 5, step S4 according to the present invention is to produce a seed-seed culture, which is prepared by putting the inoculum prepared in step S2 into a sterilized container together with a needle, followed by secondary culture.

In addition, it is preferable that the seed culture is secondary cultured for 20 to 30 days in step S4.

On the other hand, since the needle is immersed in the nutrient solution through step S1, the secondary culture in step S4 is well performed due to the nutrient components impregnated in the needle.

6 is a perspective view and a cross-sectional view showing a state in which a seed culture is inserted into a growth medium according to the present invention.

Referring to FIG. 6, step S5 according to the present invention is a step of inserting a seed culture into a growth medium for a tertiary culture, wherein the seed culture is inoculated into the growth medium using the seed culture of the first culture and the second culture.

The growth medium is obtained by mixing oak sawdust and rice bran (rice bran) at a predetermined weight ratio, for example, 8: 2 by weight, and sealing the bag.

In addition, the growth medium is filled in the bag member to have a rectangular tubular shape. Specifically, the growth medium has a height of 7 to 10 cm and a length of 30 to 50 cm.

The growth medium is placed on the floor and inserted into the seedling seedlings at predetermined intervals. At this time, the seedling seedlings are inserted in a state in which the bagging area at the position where the seedling seedling is inserted is previously disinfected with alcohol or the like.

After the seed culture is inserted, the band member is attached to the hole formed in the sealing member.

However, it is preferable that the band member is configured to be detachable and ventilated.

Specifically, the band member may include a vent portion formed of one of a fabric, a cotton and a sponge so as to be ventilated at one side, and an adhesive portion formed on one surface of the vent portion and detachably fixed to the sealing member.

The ventilation portion covers all of the holes, and the adhesive portion is formed in a sheet-like shape, and fine holes are formed to smooth the ventilation.

The tertiary culture in the step S5 is only about 30 to 40 days, which is about 1/3 of that in the conventional encapsulation culture.

FIG. 7 is a cross-sectional view showing a state in which a nutrition needle is inserted into a growth medium in step S5 according to the present invention. FIG.

Referring to FIG. 7, in step S5, when the mushroom microorganisms are deposited on the growth medium, the microorganism is removed and the microorganism is further cultured in a state where the nutrient needle is additionally inserted into the removed microorganism.

The nutritional needles can be exemplified by the needles being boiled for 30 to 60 minutes in a nutrient solution prepared by diluting a wood vinegar solution.

In particular, if the wood is dense, such as oak, it will be sterilized if it is boiled with boiled vinegar.

FIG. 8 is a cross-sectional view showing a state in which a groove is formed in a necklace according to the present invention.

Referring to FIG. 8, the needle may be formed to have a long groove extending from the upper end to the lower end.

In the step S5, when the mushroom microorganism is deposited on the growth medium, the nutrition needle may be inserted into the groove, or the third culture may be performed while the nutrient solution is injected into the groove.

As described above, when the nutritional needle is directly inserted into the growth medium or inserted into the groove formed in the needle, the yield of the mushroom can be increased and the period of cultivation can be shortened. In particular, And it is more effective when cultivating and harvesting shiitake mushroom several times in the same medium.

Hereinafter, the method for cultivating shiitake mushroom using the seed germ according to the present invention will be described in more detail with reference to preferred embodiments.

1. The oak ends are sharpened, processed into wood with a diameter of 0.7 cm and a length of 6 cm, and then immersed in nutrient solution containing wood vinegar to prepare a needle.

2. Prepare an inoculated source of shiitake mushroom inoculated on a liquid medium consisting of 20% by weight of sugar, 2% by weight of starch and a residual water for 15 days.

3. Place the needle in a plastic bag and sterilize in a sterilizer at 121 ° C for 70 minutes. At this time, an opening of the plastic bag is provided with a cap capable of ventilation.

4. Place the inoculum in a sterilized plastic bag containing a needle, and incubate for 22 days.

5. A growth medium comprising oak sawdust and rice bran (rice bran) at a weight ratio of 8: 2 is prepared, and the growth medium is sealed in a bag. The growth medium is sterilized at 120 DEG C for 90 minutes. At this time, the growth medium has a diameter of 8 to 9 cm and a length of 40 cm.

6. Three mature seedlings were inserted into the growth medium at intervals of 10 cm, and 210 g of mushroom was harvested in 35 days.

While the present invention has been described in connection with certain exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Therefore, it is to be understood that the present invention is not limited to the above-described embodiments. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims. It is also to be understood that the invention includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

Claims (7)

In the cultivation method of shiitake mushroom,
A step S1 of immersing a bar-shaped tree in a nutrient solution containing a wood vinegar to prepare a needles;
Step S2 of preparing an inoculation source inoculated with a seed medium in a liquid medium comprising sugar, starch and water and culturing the seed medium for 10 to 20 days;
Sterilizing the needle;
(B) culturing the inoculated seedlings in a sterilized container together with the sterilized needles and incubating them for 20 to 30 days;
And a step S5 of culturing the seed culture by inserting the seed culture into a growth medium,
The above-mentioned needles are obtained by immersing the tree in the nutrient solution for 12 hours to 2 days,
Wherein the nutrient solution is diluted with a mixture of wood vinegar and water at a weight ratio of 1 / 250-1 / 150.

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