KR20160087512A - Cultivating method of tree ear and the composition of cultur medium - Google Patents

Abstract

The purpose of the present invention is to provide a method for cultivating Auricularia auricula-judae and a culture medium composition for cultivating Auricularia auricula-judae which contribute to income increases of mushroom cultivators by giving the vitality of spawns inoculated into a culture medium, preventing the contamination of spoilage bacteria, promoting the growth of mushrooms, and increasing an amount of harvest. A method for cultivating Auricularia auricula-judae according to the present invention comprises: a step of preparing a culture medium comprising corncobs, wheat bran, cottonseed hull, gypsum, urea, calcium, and sulfur; a step of putting the culture medium into a culture container, sealing the culture container containing the culture medium, heating the culture container to sterilize the culture medium, and cooling the heated culture container to cool the culture medium; a step of inoculating Auricularia auricula-judae spawns into the cooled culture medium; a step of performing a dark cultivation process on the Auricularia auricula-judae spawns to cultivate mycelia; a step of performing a bright cultivation process on the Auricularia auricula-judae mycelia passed through the dark cultivation process; and a step of putting the culture container comprising the mushroom mycelia into cultivation facilities to cultivate the mushroom mycelia into mushrooms.

Description

TECHNICAL FIELD [0001] The present invention relates to a method of cultivating a mushroom,

More particularly, the present invention relates to a method for cultivating a mushroom, and more particularly, to a mushroom cultivation method and a mushroom cultivation method which can increase the yield of the mushroom, The present invention relates to a method of cultivation of a mushroom and a medium composition which contribute to the improvement of income of the mushroom.

In general, mushrooms are nutrient-requiring microorganisms that do not have photosynthesis but utilize organic carbon.

Mushroom belonging to the basidiomycetes has fruiting body that grows and develops by decomposing various organic materials. It is a food material which has a higher nutritional value than ordinary vegetables, and is a typical health food which is known to excel in hypertension, diabetes and anticancer effect It is one.

In recent years, as the level of living has improved and interest in the public health has increased, interest in concentrating clean agricultural products and high nutritional foods has been increasing, and efforts have been made to add the desired nutrients, elements or substances to existing agricultural products, Is continuing.

On the other hand, as a method of cultivating these mushrooms, there is known a method of growing the logs using hardwoods such as hardwoods and a method of cultivating them in facilities having certain facilities. However, the above-mentioned timber cultivation method is easy to manage and has a small facility cost. On the other hand, the recovery rate of mushroom is low compared to the amount of raw material, and it is difficult to purchase timber. Recently, Is growing rapidly in Korea.

At this time, in the aforementioned facility cultivation method, the medium produced by inoculating the seeds into the sawdust medium is usually used, and the sawdust medium itself is packed in a box to carry out single-phase cultivation. Since such a single-phase cultivation is a well- Is omitted. In addition, the cultivation method is described in detail in Korean Patent Laid-Open Publication No. 85-909 (Method for cultivating shiitake using hardwood sawdust) and Publication No. 90-7303 (Method for cultivating sawdust in an elevation).

According to the conventional single-phase cultivation method, the vitality of seed bacterium is lowered, the problem of contamination by germs and fungi is not solved, and only a uniform product which can not differentiate from conventional mushrooms can be harvested.

As the trend of rural agriculture and the aging of the labor force are intensifying, mushroom cultivation is limited in economic efficiency, and scientific and high value added mushroom cultivation is urgently needed.

DISCLOSURE Technical Problem The present invention has been conceived to solve the problems of the prior art as described above, and it is an object of the present invention to provide vigor of seeds inoculated on a medium, to prevent pollution of germs and to promote growth of mushroom, And to provide a cultivation method and a culture medium composition of a mushroom which contributes to the promotion of income of a mushroom cultivator.

The technical problem of the present invention as described above is achieved by the following means.

(1) preparing a culture medium containing corn cob, bran, cottonseed, gypsum, urea, calcium and sulfur; The medium is sealed in a culture container, and then heated and cooled for sterilization. Inoculation of the throat mushroom seeds into the cooled medium; Culturing the Mycelium of Mycobacterium tuberculosis through mycelial culture; Culturing the mycelia of the throat after being cultured in the dark; And a mushroom mycelium in a cultivation vessel and cultivating the mushroom with a mushroom.

(2) In the above (1)

The medium is composed of 40 to 50 wt% of corncob, 20 to 30 wt% of bran, 20 to 25 wt% of cottonseed, 2 to 5 wt% of gypsum, 0.1 to 1.0 wt% of urea, 0.1 to 0.5 wt% of calcium, 0.1% by weight, based on the total weight of the mushroom.

(3) In the above (2)

And 5 to 10% by weight of peat moss.

(4) In the above (3)

And 1 to 5% by weight of a wood vinegar.

(5) In the above (4)

And 1 to 5% by weight of a silk peptide.

(6) The mushroom grown by any one of the above methods.

(7) A flame retardant composition comprising 40 to 50 wt% of corncob, 20 to 30 wt% of bran, 20 to 25 wt% of cottonseed, 2 to 5 wt% of gypsum, 0.1 to 1.0 wt% of urea, 0.1 to 0.5 wt% To 0.1% by weight of the composition.

(8) In the above (7)

Peat moss 5-10 wt%, wood vinegar 1-5 wt%, and silk peptide 1-5 wt%.

(9) In the above (7)

And 0.1 to 1% by weight of an ethanol extract or extract of garlic.

As described above, according to the present invention, it is possible to increase the yield of the mushroom cultivation farmer by enhancing the vigor of the seedlings inoculated on the culture medium, preventing the contamination of the microbes and promoting the growth of the mushroom.

FIG. 1 is a flow chart of cultivation of a mushroom using a medium according to the present invention.

The method for growing mushroom using the sawdust medium according to the present invention comprises:

Preparing a culture medium containing corn cob, bran, cottonseed, gypsum, urea, calcium and sulfur; The medium is sealed in a culture container, and then heated and cooled for sterilization. Inoculation of the throat mushroom seeds into the cooled medium; Culturing the Mycelium of Mycobacterium tuberculosis through mycelial culture; Culturing the mycelia of the throat after being cultured in the dark; And a mushroom mycelial body, into a grower, and culturing the mushroom with the mushroom.

Hereinafter, the contents of the present invention will be described in more detail with reference to the embodiment shown in FIG.

The sawdust medium for mushroom cultivation according to the present invention preferably contains 40 to 50 wt% of corncob, 20 to 30 wt% of wheat bran, 20 to 25 wt% of cottonseed, 2 to 5 wt% of gypsum, 0.1 to 1.0 wt% of urea, 0.1 to 0.5% by weight, and 0.01 to 0.1% by weight sulfur.

When the content of each component is below the lower limit value, it is difficult to reduce the yield of the mushroom or to provide the desired physiological activity, and the content of each component If the upper limit of the range is exceeded, there is a risk that the yield will be lowered.

Accordingly, in a preferred embodiment of the present invention, there is provided a method for producing a gypsum comprising 40 to 50 wt% of corncob, 20 to 30 wt% of bran, 20 to 25 wt% of cottonseed, 2 to 5 wt% of gypsum, 0.1 to 1.0 wt% 0.5% by weight, and 0.01% by weight to 0.1% by weight sulfur, respectively, to satisfy the optimal conditions for both enhancing vitality and increasing yield of the mushroom.

In the present invention, not only staining due to blue mold can be prevented by adding sulfur to the sawdust medium, but also by allowing the sulfur component to migrate to the inside of the mushroom, the anticancer action and various skin diseases , Tack recovery, cholesterol synthesis inhibition and thrombolysis, bone strengthening, inflammation elimination and sterilization, diuretic and constipation inhibiting action, and diabetic disease improvement.

The sulfur content is preferably 0.01 to 0.1 wt%, and if it is added in an amount of less than 0.01 wt%, the migration rate of sulfur is significantly lowered. If the content is more than 0.1 wt%, the growth of the mushroom may be inhibited, And added in the above range.

The sawdust medium according to the present invention may further comprise at least one or more components selected from the group consisting of peat moss, vinegary and silk peptides. The content of the peat moss, vinegar solution and silk peptide is not particularly limited, but it is preferably 5 to 10% by weight of peat moss, 1 to 5% by weight of a vinegar solution and 1 to 5% by weight of a silk peptide.

By adding the peat moss, vinegar solution and silk peptide to the medium, it is possible to more completely block contamination from germs and fungi, and it is possible to sufficiently supply various minerals and nutrients necessary for the growth of mushroom, The present invention provides an effect of further increasing the power consumption.

In order to provide the mushroom with a selenium component which is well known to enhance immunological activity, the present invention preferably further comprises 0.1 to 1% by weight of the ethanol extract or extract of garlic based on the weight of the culture composition.

As shown in FIG. 1, the method of cultivating mushroom using the sawdust medium according to the present invention comprises the steps of preparing a culture composition comprising a corncob, wheat bran, cottonseed, gypsum, urea, calcium and sulfur, Is required.

The thus prepared culture medium is placed in a culture container and the culture container is placed in a sterilizer and incubated at 90 to 110 ° C, preferably 100 ° C for 50 to 70 minutes, preferably 60 minutes, 120 to 125 ° C, preferably 121 Deg.] C for 80 to 100 minutes, preferably 90 minutes, and then the medium is cooled to 20 to 25 [deg.] C to prepare a medium.

The method of growing the mushroom using the sterilized medium as described above is as follows.

The inoculated medium is inoculated at a temperature of 20 to 25 DEG C, preferably 21 to 22 DEG C, and a humidity of 50% or more, preferably 60 to 70% at 30 To 60 days, preferably 30 days.

After dark culture is terminated, light cultivation is carried out by providing sunlight for 30 to 60 days, preferably 30 days. Thus, after light cultivation is completed, the seedlings are placed on the grower.

According to the present invention, the mushroom can be harvested after culturing for about 5 to 8 days, preferably one week, after incidence.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood, however, that these examples are provided so that the scope of the present invention is not limited thereto.

[Example 1]

720 kg of corncob, 360 kg of wheat bran, 360 kg of cottonseed, 42 kg of gypsum, 4.8 kg of urea, 5.0 kg of calcium and 1.0 kg of sulfur were blended to form a medium for cultivation of 1400 thorny mushrooms.

[Example 2]

620 kg of koncove, 100 kg of peat moss, 360 kg of wheat bran, 360 kg of cottonseed, 42 kg of gypsum, 4.8 kg of urea, 5.0 kg of calcium and 1.0 kg of sulfur were blended to form 1400 thistle mushroom culture media.

[Example 3]

620 kg of corncob, 100 kg of peat moss, 25 kg of silk peptide, 335 kg of wheat bran, 360 kg of cottonseed, 42 kg of gypsum, 4.8 kg of urea, 5.0 kg of calcium and 1.0 kg of sulfur were mixed to form a medium for cultivation of 1400 thorny mushrooms .

[Example 4]

1400 sows were cultivated by mixing 620 kg of konkov, 100 kg of peat moss, 25 kg of silk peptide, 25 kg of wood vinegar, 335 kg of wheat bran, 335 kg of cottonseed, 42 kg of gypsum, 4.8 kg of urea, 5.0 kg of calcium and 1.0 kg of sulfur Medium was established.

[Example 5]

10 kg of garlic extract and 10 kg of garlic extract were added to a mixture of 620 kg of konkov, 100 kg of peat moss, 25 kg of silk peptide, 25 kg of wood vinegar, 335 kg of bran, 335 kg of cottonseed, 42 kg of gypsum, 4.8 kg of urea, To form a medium for cultivation of 1400 throat mushrooms.

[Experimental Example 1] Cultivation of thrips

The medium prepared in Examples 1 to 5 was placed in a cylindrical plastic bag having a diameter of 14 cm and a height of 12 cm and bundled. The medium container was placed in a sterilizer and sterilized at 100 ° C for 60 minutes at 121 ° C for 90 minutes, and then the medium was cooled to 20 to 25 ° C.

After the inoculated seeds were inoculated into the sterilized medium as described above, the inoculated medium was cultured for 30 days at 22 ° C and 60% humidity for 30 days. After termination of the arm cultivation, the medium was further cultured for 30 days, and then cultured for 1 week in a grower, and the mushroom was harvested.

As a comparative example, when the mushroom was cultivated under the same conditions using oak sawdust and commercial sawdust medium containing the rice bran as the main raw material, the yield was 11% on average in Example 1, 13% in Example 2, An increase of 15% and 17%, respectively, for Example 4 was observed.

[Experimental Example 2] Sulfur content

The compositions of the sulfurous amino acids in the mushroom were compared with those of the mushroom according to the comparative example. The results are shown in Tables 1 and 2, respectively.

(Unit: wet basis, mg / g) Sample Cysteine Methionine Example 1 0.128 0.007 Example 2 0.131 0.009 Example 3 0.133 0.013 Example 4 0.135 0.017 Example 5 0.140 0.022 Comparative Example 0.074 0.003

As described above, cultivation of the mushroom using the culture medium according to the present invention showed that the cultivation period was shortened and the yield was increased, as well as the sulfur amino acid was increased, so that the mushroom was well absorbed into the mushroom of the sulfur component. In case of Example 5, it was judged that the sulfuric amino acid content was larger due to the supply of the allysine component, which is a sulfur compound of garlic.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. It can be understood that

Claims (9)

Preparing a culture medium containing corn cob, bran, cottonseed, gypsum, urea, calcium and sulfur; The medium is sealed in a culture container, and then heated and cooled for sterilization. Inoculation of the throat mushroom seeds into the cooled medium; Culturing the Mycelium of Mycobacterium tuberculosis through mycelial culture; Culturing the mycelia of the throat after being cultured in the dark; And culturing the mushroom with a mushroom, wherein the mushroom is cultivated in a cultivation vessel containing the mushroom mycelium. The method according to claim 1,
The medium is composed of 40 to 50 wt% of corncob, 20 to 30 wt% of bran, 20 to 25 wt% of cottonseed, 2 to 5 wt% of gypsum, 0.1 to 1.0 wt% of urea, 0.1 to 0.5 wt% of calcium, 0.1% by weight, based on the total weight of the mushroom. 3. The method of claim 2,
And 5 to 10% by weight of peat moss. The method of claim 3,
And 1 to 5% by weight of a wood vinegar. 5. The method of claim 4,
And 1 to 5% by weight of a silk peptide. A mushroom grown by the method of any one of claims 1 to 5. 20 to 30% by weight of bark, 20 to 25% by weight of cottonseed, 2 to 5% by weight of gypsum, 0.1 to 1.0% by weight of urea, 0.1 to 0.5% by weight of calcium and 0.01 to 0.1% by weight of sulfur Lt; RTI ID = 0.0 >% < / RTI > 8. The method of claim 7,
Peat moss 5-10 wt%, wood vinegar 1-5 wt%, and silk peptide 1-5 wt%. 8. The method of claim 7,
And 0.1 to 1% by weight of an ethanol extract or extract of garlic.

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