CN103724058B - Microbial fermentation functional organic compound fertilizer and production method thereof - Google Patents
Microbial fermentation functional organic compound fertilizer and production method thereof Download PDFInfo
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- CN103724058B CN103724058B CN201410011311.8A CN201410011311A CN103724058B CN 103724058 B CN103724058 B CN 103724058B CN 201410011311 A CN201410011311 A CN 201410011311A CN 103724058 B CN103724058 B CN 103724058B
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Abstract
The invention relates to a microbial fermentation functional organic compound fertilizer and a production method thereof. The method comprises the following steps of: mixing lactobacillus buchneri (i) Lactobacillusbuchneri (/i) CICC (China Center of Industrial Culture Collection) 6004, bacillus cereus (i) Bacilluscereus (/i) ACCC (Agricultural Culture Collection of China) 10117, saccharomycescerevisiae (i) Saccharomyces (/i) (i) cerevisiae (/i) CICC1905, and serratia marcescent (i) Serratia (/i) (i) marcescent (/i) ACCC10119; and fermenting to produce an bio-organic fertilizer. With the adoption of the mixing-fermenting method, the estrogen and medicine residues in excrements of livestocks can be effectively degraded; nematode and fly maggot in the soil can also be eliminated; in addition, the shelf life of the organic fertilizer and the survival time of viable organism can be greatly prolonged, and the germination rate and stress resistance of crops seeds can also be improved, etc.
Description
Technical field
The present invention relates to bioengineering field, relate to a kind of Microbial fermentation functional organic compound fertilizer and production method thereof.
Background technology
The development of the ecological agriculture is extremely focused in the development of modern agriculture, and the arviculture technology of high yield and high quality be unable to do without fertilizer." agriculture YIZHIHUA, complete in fertile main ".The effect of Appropriate application in modern agricultural production of fertilizer is more and more important, and Organic farming becomes agricultural development direction from now on.We know, the high speed development of agricultural be unable to do without the use of chemical fertilizer, but due to the soil compaction abused chemical fertilizer and cause long-term in agriculture production, environmental pollution and quality of agricultural product decline, and also cause the deterioration of natural ecological environment simultaneously.And fertilizer has the irreplaceable special role of chemical fertilizer, it is the essential condition of Sustainable Agricultural high and stable yields.It contains abundant organic matter, can improve the water conservation of soil, fertilizer conservation and ventilation capacity, and therefore in the process of Agriculture Production, organic fertilizer plays an important role always.
Biological organic fertilizer be generally with the solid waste of feces of livestock and poultry, stalk, agricultural byproducts and food-processing for raw material, be equipped with fermented bacterium fermentation processing that is single or several functions and form, the organic fertilizer containing a certain amount of functional microbial.It is neither traditional fertilizer, and microbial-bacterial fertilizer that neither be simple is the organic combination of the two, effectively can improve the utilization ratio of fertilizer, strengthens crop root vigor and nutrient absorption capability.Therefore, acquisition high grade and yield of crops and one of important measures of increasing soil fertility are not only in research and development also Rational Application bio-organic fertilizer, also preserve the ecological environment, promote the inexorable trend of agricultural sustainable development.
But the production of existing biological organic fertilizer all be unable to do without feces of livestock and poultry, because content organic in feces of livestock and poultry is very high, the nutrient contents such as nitrogen, phosphorus, potassium are also higher, are therefore very suitable for the fertilizer of production high-quality.But, containing a large amount of objectionable impuritiess in the ight soil of livestock and poultry, the pathogenic micro-organism in ight soil, as: avian influenza virus, horsepower kirschner virus etc., these viral micro-organisms can enter soil and shallow ground water with the water in ight soil, the harm environment that meeting prolongation is in time serious; Some growth promoters simultaneously in animal and fowl fodder, anticoccidiosis medicine, disease-resistant drug can excrete with ight soil, infiltrates in water and is drunk by people by surface water or underground water, cause drug residue and heavy metal poisoning, the water pollution that feces of livestock and poultry produces thus is quite serious.
In addition, chicken manure is untreated to be applied directly in farmland for a long time, by the enrichment causing phosphorus, copper, zinc and trace element thereof, medicine in soil, thus produces toxic action to crop, has a strong impact on growing of crop, crop failure; Although carried out the comprehensive development and utilization to feces of livestock and poultry at present, alleviate the harm of feces of livestock and poultry to environment to a certain extent, but along with improving constantly of people's living standard, the demand of meat egg product is being increased, the sterol material such as oestrogenic hormon, cholesterol in laying hen, dam body enters in environment along with ight soil, cause the severe contamination of environment, add hormone and also to have increased weight widely the pollution of hormone to environment as the use of fodder additives.According to reports, the hormonal activity of steroid substances is much higher than other environmental estrogens class material, and it is mainly derived from the movement of human body and livestock, and these hormones can disturb endocrine dysfunction that is biological and human normal, the sex hormone secretion amount of organism and human body and activity and sperm quantity is caused to reduce, the sickness rate such as the abnormal cancer of reproductive organ increase, and reproductive performance is reduced, the health of offspring and surviving rate decline, and steroid richness amasss persistent existence and the procreation of environmental degradation serious threat biology and the mankind caused.
In addition, it is short that current biological fermentation fertilizer also exists the quality guaranteed period, and the survival benefit of effective microbe is poor; And bacterium quantity keeps in microbiological fermented organic fertilizer, the length of the survival time of bacterium is the important indicator of microbial fertilizer, bacterium survival time is long, active strong, the bacterial strain that accommodative ability of environment is strong with existence colonization ability is the key problem in technology producing biological organic fertilizer, is also the basic guarantee of producing biological organic fertilizer on a large scale.
Oestrogenic hormon contained in the biological organic fertilizer of current production, drug residue are quite high, serious threat is constituted to environment and the healthy of people, how to reduce the impact of this threat environmental pollution, food safety and mankind itself's health, find new having and promote that the green micro organism fertilizer of plant growth effect becomes problem demanding prompt solution.
Summary of the invention
The present invention is directed to many weak points that existing microbial organic fertilizer exists, provide a kind of Microbial fermentation functional organic compound fertilizer and producer thereof, adopt after adopting the method for mixed fermentation simultaneously, the oestrogenic hormon that can effectively degrade in feces of livestock and poultry, drug residue, the nematode in soil and fly maggot can also be killed simultaneously, and substantially increase the quality guaranteed period of fertilizer and the survival time of viable bacteria, the percentage of germination of raising crop seed and crop anti-adversity etc.
Concrete technical scheme of the present invention is: a kind of production method of Microbial fermentation functional organic compound fertilizer, as follows:
Below operate and all aseptically carry out,
(1) cultivation of first class inoculum
The weight percentage of each component of first class inoculum substratum is:
Industry whey powder 2%, industrial Sodium.alpha.-hydroxypropionate 0.4%, industrial anhydrous sodium acetate 0.1%, industrial proteins peptone 1%, bean cake powder 3%, cerelose 1.5%, yeast leaching powder 0.5% and water 91.5%, pH value nature;
Concrete operations: adopt 500mL triangular flask, the in-built above substratum 200mL dissolved of bottle, first adds Lactobacillus buchneri
lactobacillus buchnericICC 6004 0.1mL, then add bacillus cereus
bacillus cereusaCCC10117 0.1mL, then seals mouth by triangular flask gauze, puts into shaking table, 140 rev/min, 37 ± 1 DEG C, cultivates 6 hours, stops shaking table, then access baker's active dried yeast in triangular flask
saccharomyces cerevisiaecICC1905 and Serratia
serratia marcescentthe each 0.1mL of ACCC 10119, and then triangular flask is sealed, put in shaking table, 140 rev/min, 32 ± 1 DEG C, cultivate 10 hours, then stop shaking table, first class inoculum is cultivated and is terminated;
(2), seed tank culture
The weight percentage of each component of seed tank culture base is:
Ammonium sulfate 0.2%, potassium primary phosphate 0.3%, industrial whey powder 1%, industrial Sodium.alpha.-hydroxypropionate 0.5%, industrial anhydrous sodium acetate 0.2%, industrial proteins peptone 1.2%, bean cake powder 4%, cerelose 1.5%, industrial yeast leaching powder 0.5%, sodium-chlor 0.5%, magnesium sulfate 0.1%, lactose 0.2%, oligochitosan 0.3% and water 89.5%, pH nature;
Concrete operations: temperature 30 ± 1 DEG C in tank, above cultured first class inoculum is aseptically inoculated in tank, inoculum size is the 2wt% of substratum in tank, sterile air ventilating fermentation, sterile air consumption is: every 100 kilograms of seed culture fluid per minute sterile airs 0.1 cube, pH nature, mixing speed 140 rev/min, fermentation culture 30 hours, microscopy is qualified without miscellaneous bacteria; After obtaining above-mentioned seed tank culture bacterial classification, this bacterial classification is utilized to adopt prior art fermentation to obtain fertilizer.
The present invention also has following technical characteristic:
1, a kind of Microbial fermentation functional organic compound fertilizer that the production method of a kind of Microbial fermentation functional organic compound fertilizer as above is obtained is adopted.
2, adopt the production method of a kind of Microbial fermentation functional organic compound fertilizer as above, or add 0.5wt% brown sugar in seed tank culture bacterial classification, after dissolving, obtained foliage fertilizer.
3, a production method for Microbial fermentation functional organic compound fertilizer, adopt the method for seed tank culture strain fermentation biological organic fertilizer as above, concrete steps are as follows:
Fill a prescription as follows: fertile for 100kg, do not comprise water and bacterial classification,
Dried poultrymanure 30kg, dry pig manure 53.4kg, the thin powdered rice hulls 10kg of 40 order, lactose 0.1kg, oligochitosan 0.5kg, wheat bran skin 6kg,
Add water 40kg after each material metage mixing to mix thoroughly, pile and park 5 hours, then the cultured seeding tank bacterial classification accounting for dry material 2wt% is added, first add after the dilution proportion of 1:3 with water before adding, limit edged turning, add after bacterial classification is mixed thoroughly and start to stack, need to pile bottom width 1.5 meters, upper wide 1.2 meters, high 1.2 meters, the long long pile do not limit, the section of buttress is trapezoidal or trilateral, adopts the method for artificial turning or mechanical turning, realizes the aerobic state in stacking;
Turning:
(1) high temperature season: during room temps 30-35 DEG C, first day builds heap, turning in second day, and in the 3rd day heap, temperature is 60 DEG C ± 5 DEG C, turning in 4th day, 5th turning limit, the remotest places add seeding tank bacterial classification in windrow, and every cube of material seeding tank bacterial classification 3kg, first adds after the dilution proportion of 1:3 with water before adding, then heap is built, lid film 36 hours, then removes film and continues fermentation 5 days, dries be finished product fertilizer with tower dryer;
(2) the temperature time in: during room temps 10-15 DEG C, first day builds heap, turning in 3rd day, seeding tank bacterial classification is added during turning in the 4th day, every cube of material seeding tank bacterial classification 3kg, first adds after the dilution proportion of 1:3 with water before adding, and then builds heap lid film after 48 hours, remove film continuation fermentation 8 days centres and will turn over 2-3 heap, be finished product fertilizer with tower dryer drying and packaging;
(3) the low temperature time: during room temps 0 DEG C, first day builds heap, turning in 3rd day, turning in the 6th day, adds seeding tank bacterial classification during turning in the 6th day, every cube of material seeding tank bacterial classification 3kg, first add after the dilution proportion of 1:3 with water before adding, then build heap lid film, turning in 96 hours once, altogether need fermentation 15 days, be finished product fertilizer with tower dryer drying and packaging.
In above-mentioned zymotechnique, the microbial strains adopted during the fermentation stable performance degradation capability is strong, and the reproduction speed of bacterium is fast.The microbial strains used, is commercially.
Wherein adopt Lactobacillus buchneri
lactobacillus buchneriafter CICC 6004, in the fermenting process of fertilizer, hormonal substance can be hydrolyzed to carbonic acid gas and water, effectively eliminate the oestrogenic hormon in fertilizer, and select bacillus cereus
bacillus cereusaCCC10117, drug residue (containing in because of feces of livestock and poultry) principal degradation colistin in the fermenting process of fertilizer in effective degraded fertilizer, duomycin, can reduce the two content greatly, and the baker's active dried yeast of the high yield trehalose that contriver adopts
saccharomyces cerevisiaecICC1905; the trehalose produced is discharged in the middle of fertilizer; the seed of trehalose to crop itself has the effect of stratification; trehalose has protection biomass cells and biologically active substance activity under the unsuitable environmental condition such as dehydration, arid, high temperature, freezing, high osmotic pressure exempts from destroyed function; and play important provide protection to biological large point of membrane protein matter and nucleic acid etc., it is necessary for adding baker's active dried yeast thus in fermenting organism fertilizer.Can secrete a large amount of trehalose of accumulation because of this yeast protects the bacterium of various microorganism in the middle of fertilizer to live and enzymic activity, extends the quality guaranteed period of fertilizer; Also can improve diastatic activity in crop, prove that this product trehalose yeast is to the cold-resistant drought-enduring performance significant role of farm crop paddy rice, wheat through test;
The Serratia adopted
serratia marcescentaCCC 10119 secretes multiple chitinase in the process of fermentation fertilizer, effectively can kill the nematode in soil and fly maggot.
In addition, contriver is when seed tank culture bacterial classification, lactose and oligochitosan is added respectively in formula, make the active substance needed for generation that bacterial classification can be more stable, lactose can be stablized baker's active dried yeast and produce more trehalose, the active dry yeast not adding lactose through test of many times produces trehalose 15%(in dry yeast), and the active dry yeast adding lactose produces trehalose 25%(in dry yeast); Fecund goes out 10%, so just substantially increases the result of use of fertilizer, extends the fertile quality guaranteed period, reduces production cost, increase economic efficiency; And after adding oligochitosan, bacillus cereus degraded fertilizer drug residue, particularly colistin and duomycin all degrade more than 90%, improve 20% with the bacillus cereus phase specific degradation rate not adding oligochitosan, Be very effective, this is also tremendous improvement compared to prior art.
The various bacterial classifications adopted, can provide nutrition for soil fungi, and the relation by forming mutualism with plant plays fixed nitrogen, phosphorus decomposing, potassium decomposing, promotion plant can produce organic nutrient; Produce a large amount of organic acid and ferment can improve disease resistance ability during fermentation simultaneously, promote plant growth.
In sum, after adopting the method for this mixed fermentation provided by the present invention, the oestrogenic hormon that can effectively degrade in feces of livestock and poultry, drug residue, the nematode in soil and fly maggot can also be killed simultaneously, and substantially increase the quality guaranteed period of fertilizer and the survival time of viable bacteria, the percentage of germination of raising crop seed and crop anti-adversity etc., a kind of functional green bio fertilizer of final acquisition.
Embodiment
Further illustrate the present invention below in conjunction with embodiment, those skilled in the art can be made more fully to understand the present invention, but do not limit the present invention in any way.
Embodiment 1:
The cultivation of first class inoculum
The weight percent of each component of first class inoculum substratum is:
Industry whey powder 2%, industrial Sodium.alpha.-hydroxypropionate 0.4%, industrial anhydrous sodium acetate 0.1%, industrial proteins peptone 1%, bean cake powder 3%, cerelose 1.5%, yeast leaching powder 0.5%, tap water 91.5%, pH value nature, above mix and blend dissolves for subsequent use.
Concrete operations: first get 500ml triangular flask, every bottled above substratum 200ml dissolved, then by triangular flask 6-8 layer gauze sealing, then with the wrapping of one deck kraft paper, puts into mediclave steam sterilizing; First connect the heating source of sterilizer, when the vapor pressure in sterilizer reaches 0.05 MPa, open the drain tap on sterilizer, discharge freezing air, evacuation time 6-8 minute; When the tensimeter on sterilizer makes zero, close drain tap, continue heating, when the vapor pressure in sterilizer reaches 0.11-0.14 MPa, keep 35 minutes, then sterilizer is left heating source, allow its Temperature fall to 30 DEG C ± 2 DEG C (prior art), then in Bechtop, open triangular flask, in each triangular flask, first add Lactobacillus buchneri
lactobacillus buchnericICC 6004 0.1mL, then add bacillus cereus
bacillus cereusaCCC10117 0.1mL, then seals mouth by triangular flask gauze, puts into shaking table 37 ± 1 DEG C, cultivate 6 hours for 140 rpms, stop shaking table, taking-up triangular flask is put into Bechtop and is opened triangular flask gauze, then accesses baker's active dried yeast in triangular flask
saccharomyces cerevisiaecICC1905 and Serratia
serratia marcescentthe each 0.1mL of ACCC 10119, and then triangular flask is sealed, to put in shaking table 32 ± 1 DEG C, 140 rpms, cultivate 10 hours, then stop shaking table, first class inoculum is cultivated and is terminated;
The sterilizing of fermentation equipment pipeline and sterilizing filter
Open each valve of each inlet and outlet piping valve and sterile air pipeline, pass into the steam of 0.12-0.14 MPa, allow steam and pipeline valve UNICOM have steam to discharge, ventilate 10 minutes, then close each drain tap, allow vapor pressure in pipeline keep 0.12-0.14 MPa, keep 40 minutes.
The slack tank sterilizing of seeding tank and fermentor tank
Close each valve, open the sewage draining valve of tank interlayer, then open the steam that open steam valve passes into 0.12-0.14 MPa in tank, timing is started when reaching 121 ± 1 DEG C in tank, sterilization time is 40 minutes, then closes flushbottom tank valve, and in tank, temperature drops between 25-35 DEG C for subsequent use.
Seed tank culture
The weight percent of each component of seed tank culture base
Ammonium sulfate 0.2%, potassium primary phosphate 0.3%, industrial whey powder 1%, industry Sodium.alpha.-hydroxypropionate 0.5%, industrial anhydrous sodium acetate 0.2%, industrial proteins peptone 1.2%, bean cake powder 4%, cerelose 1.5%, industrial yeast leaching powder 0.5%, sodium-chlor 0.5%, magnesium sulfate 0.1%, lactose 0.2%, oligochitosan 0.3%, tap water 89.5%, pH nature.
Concrete operations: first the water used in formula is put in the tank of slack tank sterilizing, and start stirrer, then the starting material in formula are added respectively, then steam heating is passed into, when utilizing jacket steam to be heated to 95 DEG C, use direct steam heating instead again, when vapor pressure in tank reaches 0.12-0.14 MPa, be incubated 35 minutes, sterilising effect can be reached, then steam off, when temperature in tank drops to 30 ± 1 DEG C, above cultured first class inoculum is aseptically inoculated in tank, inoculum size is the 2wt% of substratum weight in tank, then inoculation lid is built, start sterile air ventilating fermentation, sterile air consumption is every 100 kilograms of seed culture fluid per minute sterile airs 0.1 cube, pH nature, mixing speed 140 turns of per minutes, fermentation culture 30 hours, microscopy is qualified without miscellaneous bacteria, more than be called seed tank culture bacterial classification.
Embodiment 2:
Utilize the method for above-mentioned seed tank culture strain fermentation biological organic fertilizer, concrete steps are as follows:
Fill a prescription as follows: fertile for 100kg, do not comprise water and bacterial classification
Dried poultrymanure 30kg, dry pig manure 53.4kg, the thin powdered rice hulls 10kg of 40 order, lactose 0.1kg, oligochitosan 0.5kg, wheat bran skin 6kg, add tap water 40kg after each material metage mixing to mix thoroughly, pile and park 5 hours, then the above cultured seeding tank bacterial classification (2wt% of dry material) of 2wt% is added, first add by after the dilution proportion of 1:3 with tap water before adding, both 1kg bacterium liquid 3kg tap water, limit edged turning, add after bacterial classification is mixed thoroughly and start to stack, need to pile bottom width 1.5 meters, upper wide 1.2 meters, high 1.2 meters, the long long pile do not limit.The section of buttress is trapezoidal or trilateral, adopts the method for artificial turning or mechanical turning, realizes the aerobic state in stacking.
Turning: when high temperature season, room temps, 30-35 DEG C, first day builds heap, turning in second day, in the 3rd day heap, temperature is 60 DEG C ± 5 DEG C, turning in the 4th day, 5th turning limit, the remotest places add bacterial classification-tap water diluent (adding in addition) that weight ratio is 1:3 in windrow, every cube of material, with pure bacterium liquid 3kg, then builds heap, lid film 36 hours, then film is removed and continue fermentation 5 days, dry with tower dryer and be finished product fertilizer.
In order to adapt to the situation of differing temps, contriver further defines: the middle temperature time: room temps, during 10-15 DEG C, first day builds heap, turning in the 3rd day, and during turning in the 4th day, turning limit, limit adds bacterial classification-tap water diluent that weight ratio is 1:3 in heap, pure bacterium liquid 3 ㎏ of every square stock, then build heap lid film after 48 hours, remove film continuation fermentation 8 days centres and will turn over 2-3 heap, be finished product fertilizer with tower dryer drying and packaging.
And low temperature season, room temps, 0 DEG C time, first day builds heap, turning in 3rd day, turning in 6th day, during turning in the 6th day, turning limit, limit weight ratio is the bacterial classification-tap water diluent of 1:3, pure bacterium liquid 3 ㎏ of every square stock, then heap lid film is built, turning in 96 hours once, altogether needs fermentation 15 days, is finished product fertilizer with tower dryer drying and packaging.
The fertilizer utilizing aforesaid method to obtain has long quality-guarantee period, viable bacteria rate is high, compared with general microbial organic fertilizer, the quality guaranteed period of general microbial organic fertilizer is 6 months, viable bacteria rate keeps 20%, and the biological organic fertilizer quality guaranteed period utilizing this technology to produce is 12 months, viable bacteria rate keeps 90%, substantially increases result of use.
The organic fertilizer product index of above-mentioned acquisition is as following table 1:
| Project | Technical indicator | |
| Living bacteria count (cfu, hundred million g) | ≥ | 500000000 |
| Organic (in butt) % | ≥ | 45 |
| Moisture % | ≤ | 15 |
| PH value | ≤ | 5-9 |
| Excrement colibacillus group is several/g | ≤ | 50 |
| Nematode and fly maggot death toll % | ≥ | 95 |
| Jin Mei Su ㎎/100g | ≤ | 3 |
| Bacillus adhaerens Su ㎎/100g | ≤ | 2 |
| Steroid hormone μ g/100g | ≤ | 2 |
| Normal temperature is preserved | ≥ | 12 months |
The quality guaranteed period of general microbial organic fertilizer is 12 months, and viable bacteria rate keeps 80%.
Prior art obtains following (commercially available prod) table 2 of organic fertilizer product index:
| Project | Technical indicator | |
| Living bacteria count (cfu, hundred million g) | ≥ | 200000000 |
| Organic (in butt) % | ≥ | 35 |
| Moisture % | ≤ | 20 |
| PH value | ≤ | 5-9 |
| Excrement colibacillus group is several/g | ≤ | 100 |
| Nematode and fly maggot death toll % | ≥ | 75 |
| Jin Mei Su ㎎/100g | ≤ | 9 |
| Bacillus adhaerens Su ㎎/100g | ≤ | 6 |
| Steroid hormone μ g/100g | ≤ | 8 |
| Normal temperature is preserved | ≥ | 6 months |
The quality guaranteed period of general microbial organic fertilizer is 6 months, and viable bacteria rate keeps 20%.
Add serratia marcescens, nematode and fly maggot death toll synopsis 3:
| Group | Nematode and fly maggot death toll |
| Add serratia marcescens | ≥95% |
| Do not add serratia marcescens | ≥75% |
Visible: by the contrast of his-and-hers watches 1, table 2, the objectionable impurities of table 1 far below table 2, this fertilizer oestrogenic hormon and drug residue low, nematode and fly maggot death toll height long quality-guarantee period, effective viable bacteria content is high.
The fertilizer utilizing above-described embodiment to obtain carries out field test:
The microbial organic fertilizer (solid) that first every mu of ground use this technology to ferment is as base fertilizer:
1. the liquid microbe fertilizer that this technology is produced is utilized, dilute by 1:30(tap water, namely 1kg liquid microbe fertilizer adds tap water 30kg) soak seed rice, soak time is 48 hours, and percentage of germination improves 55% compared with control group.Seedling growth is even, well developed root system, after cultivation to Post flowering 3 days again with this liquid microbe bacterial manure foliage spray 1 time, every mu of ground 4kg Liquid Fertilizer dilutes 30 times, weighs, during results compared with control group, with the full seed of this fertilizer, every mu of volume increase 110 kilograms, paddy rice, have received good benefit.
2. by weight 1:30 times of diluted liquid fertilizer, leaching Wheat Species 2 hours, then sows, the sprouting of effective promotion wheat seed, improves wheat seed germinating rate, Miao Zhuan, well developed root system, within 5 days after wheat flower, press 1:30 times of foliage spray once, use 3kg Liquid Fertilizer for every mu, results are weighed compared with control group, experimental group wheatland wheat does not lodge, evenly, full seed, every mu is increased production 85 kilograms in growth.
3. apple thank spend after 5 days by weight 1:30 times of diluted liquid fertilizer, then carry out foliage spray, young fruit period foliage spray 1 time again, compared with control group, generally can increase production 25%, pol is high, for shelf-stable, can shift to an earlier date listing in 15 days.
4. after 1:30 times of diluted liquid fertilizer, soak seed of Fructus Cucumidis sativi 8 hours, can promote seed germination, Miao Zhuan, Post flowering 2 days foliage spray 1:30 times diluents can improve cucumber yield 25%.
Experimental example 3:
After contriver adds lactose and chitosan, contrast the impact of net result, result is as follows:
the active dry yeast adding lactose produces the contrast table 4 of trehalose
Add oligochitosan aborning, improve the effect of short lactobacillus degraded hormone, also improve the effect of bacillus cereus degraded drug residue simultaneously.
estrogenic mensuration effect table 5 in short lactobacillus degraded fertilizer
| Group | Degraded proestrogen content | Estrogen content after degraded | Degradation rate |
| Test group | 100g fertilizer is containing oestrogenic hormon 100 μ g | 100g fertilizer is containing oestrogenic hormon 30 μ g | 70% |
| Control group | 100g fertilizer is containing oestrogenic hormon 100 μ g | 100g fertilizer is containing oestrogenic hormon 100 μ g | 0 |
add the measurement result table 6 of the strain fermentation fertilizer hormone of oligochitosan
wax-like gemma bar degraded medicine residue analysis result table 7 in fertilizer
add the bacillus cereus degraded fertilizer Chinese medicine residue analysis result table 8 of oligochitosan
Visible, add the bacterial classification of lactose and oligochitosan cultivation, make the active substance needed for generation that bacterial classification can be more stable, lactose energy stabilizing active dry yeast produces more trehalose, the active dry yeast not adding lactose through test of many times produces trehalose 15%(in dry yeast), and the active dry yeast adding lactose produces trehalose 25%(in dry yeast); Fecund goes out 10%, so just substantially increases the result of use of fertilizer, extends the fertile quality guaranteed period, reduces production cost, increase economic efficiency; And after adding chitosan, bacillus cereus degraded fertilizer drug residue, particularly colistin and duomycin all degrade more than 90%, improve 20% with the bacillus cereus degradation rate not adding oligochitosan, Be very effective, this is also tremendous improvement compared to prior art.
Claims (3)
1. a production method for Microbial fermentation functional organic compound fertilizer, is characterized in that, method is as follows:
Concrete formula is as follows: fertile for 100kg, does not comprise water and bacterial classification,
Dried poultrymanure 30kg, dry pig manure 53.4kg, the thin powdered rice hulls 10kg of 40 order, lactose 0.1kg, oligochitosan 0.5kg, wheat bran skin 6kg,
Add water 40kg after each material metage mixing to mix thoroughly, pile and park 5 hours, then the cultured seeding tank bacterial classification accounting for dry material 2wt% is added, first add after the dilution proportion of 1:3 with water before adding, limit edged turning, add after bacterial classification is mixed thoroughly and start to stack, need to pile bottom width 1.5 meters, upper wide 1.2 meters, high 1.2 meters, the long long pile do not limit, the section of buttress is trapezoidal or trilateral, adopts the method for artificial turning or mechanical turning, realizes the aerobic state in stacking;
Turning:
(1) high temperature season: during room temps 30-35 DEG C, first day builds heap, turning in second day, and in the 3rd day heap, temperature is 60 DEG C ± 5 DEG C, turning in 4th day, 5th turning limit, the remotest places add seeding tank bacterial classification in windrow, and every cube of material seeding tank bacterial classification 3kg, first adds after the dilution proportion of 1:3 with water before adding, then heap is built, lid film 36 hours, then removes film and continues fermentation 5 days, dries be finished product fertilizer with tower dryer;
(2) the temperature time in: during room temps 10-15 DEG C, first day builds heap, turning in 3rd day, seeding tank bacterial classification is added during turning in the 4th day, every cube of material seeding tank bacterial classification 3kg, first adds after the dilution proportion of 1:3 with water before adding, and then builds heap lid film after 48 hours, remove film continuation fermentation 8 days centres and will turn over 2-3 heap, be finished product fertilizer with tower dryer drying and packaging;
(3) the low temperature time: during room temps 0 DEG C, first day builds heap, turning in 3rd day, turning in the 6th day, adds seeding tank bacterial classification during turning in the 6th day, every cube of material seeding tank bacterial classification 3kg, first add after the dilution proportion of 1:3 with water before adding, then build heap lid film, turning in 96 hours once, altogether need fermentation 15 days, be finished product fertilizer with tower dryer drying and packaging;
Wherein said seeding tank bacterial classification, cultural method is as follows, below operates and all aseptically carries out,
(1) cultivation of first class inoculum
The weight percentage of each component of first class inoculum substratum is:
Industry whey powder 2%, industrial Sodium.alpha.-hydroxypropionate 0.4%, industrial anhydrous sodium acetate 0.1%, industrial proteins peptone 1%, bean cake powder 3%, cerelose 1.5%, yeast leaching powder 0.5% and water 91.5%, pH value nature;
Concrete operations: adopt 500mL triangular flask, the in-built above substratum 200mL dissolved of bottle, first add Lactobacillus buchneri (
lactobacillus buchneri) CICC 6004 0.1mL, then add bacillus cereus (
bacillus cereus) ACCC10117 0.1mL, then triangular flask gauze is sealed mouth, puts into shaking table, 140 rev/min, 37 ± 1 DEG C, cultivate 6 hours, stop shaking table, then access in triangular flask baker's active dried yeast (
saccharomyces cerevisiae) CICC1905 and Serratia (
serratia marcescent) each 0.1mL of ACCC 10119, and then triangular flask is sealed, put in shaking table, 140 rev/min, 32 ± 1 DEG C, cultivate 10 hours, then stop shaking table, first class inoculum is cultivated and is terminated;
(2), seed tank culture
The weight percentage of each component of seed tank culture base is:
Ammonium sulfate 0.2%, potassium primary phosphate 0.3%, industrial whey powder 1%, industrial Sodium.alpha.-hydroxypropionate 0.5%, industrial anhydrous sodium acetate 0.2%, industrial proteins peptone 1.2%, bean cake powder 4%, cerelose 1.5%, industrial yeast leaching powder 0.5%, sodium-chlor 0.5%, magnesium sulfate 0.1%, lactose 0.2%, oligochitosan 0.3% and water 89.5%, pH nature;
Concrete operations: temperature 30 ± 1 DEG C in tank, above cultured first class inoculum is aseptically inoculated in tank, inoculum size is the 2wt% of substratum in tank, sterile air ventilating fermentation, sterile air consumption is: every 100 kilograms of seed culture fluid per minute sterile airs 0.1 cube, pH nature, mixing speed 140 rev/min, fermentation culture 30 hours, microscopy is qualified without miscellaneous bacteria.
2. the production method of a kind of Microbial fermentation functional organic compound fertilizer according to claim 1, is characterized in that: or 0.5wt% brown sugar is added in seed tank culture bacterial classification, after dissolving, obtained foliage fertilizer.
3. a kind of Microbial fermentation functional organic compound fertilizer that the production method of a kind of Microbial fermentation functional organic compound fertilizer according to claim 1 is obtained.
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| CN106701646B (en) * | 2017-03-30 | 2019-05-24 | 广东省农业科学院农业资源与环境研究所 | One plant of tetracycline degradation bacteria and its application in livestock excrement compostingization processing |
| CN108046859A (en) * | 2017-12-15 | 2018-05-18 | 北京泰然悦斯莱特农业科技发展有限公司 | The preparation method of organic fertilizer fermentation agent, organic fertilizer and the two |
| CN111793585B (en) * | 2020-08-12 | 2023-06-30 | 杭州新原起生物科技有限公司 | Lactobacillus buchneri, culture method thereof and application thereof in agricultural planting |
| CN114107128A (en) * | 2021-12-15 | 2022-03-01 | 四川汇邦环保科技有限公司 | Microbial agent for degrading colistin sulfate and application thereof |
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