CN103724058A - Microbial fermentation functional organic compound fertilizer and production method thereof - Google Patents
Microbial fermentation functional organic compound fertilizer and production method thereof Download PDFInfo
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Abstract
The invention relates to a microbial fermentation functional organic compound fertilizer and a production method thereof. The method comprises the following steps of: mixing lactobacillus buchneri (i) Lactobacillusbuchneri (/i) CICC (China Center of Industrial Culture Collection) 6004, bacillus cereus (i) Bacilluscereus (/i) ACCC (Agricultural Culture Collection of China) 10117, saccharomycescerevisiae (i) Saccharomyces (/i) (i) cerevisiae (/i) CICC1905, and serratia marcescent (i) Serratia (/i) (i) marcescent (/i) ACCC10119; and fermenting to produce an bio-organic fertilizer. With the adoption of the mixing-fermenting method, the estrogen and medicine residues in excrements of livestocks can be effectively degraded; nematode and fly maggot in the soil can also be eliminated; in addition, the shelf life of the organic fertilizer and the survival time of viable organism can be greatly prolonged, and the germination rate and stress resistance of crops seeds can also be improved, etc.
Description
Technical field
The present invention relates to bioengineering field, relate to a kind of microorganism Fermentation Function compoiste fertilizer and production method thereof.
Background technology
The development of the ecological agriculture is extremely focused in the development of modern agriculture, and the arviculture technology of high yield and high quality be unable to do without fertilizer." agriculture YIZHIHUA, complete in fertile main ".The effect of the reasonable utilization of fertilizer in modern agricultural production is more and more important, and Organic farming becomes agricultural development direction from now on.We know, the use of the too busy to get away chemical fertilizer of high speed development of agricultural, but due to the soil compaction that in agriculture production, long-term abuse chemical fertilizer causes, environmental pollution and quality of agricultural product decline, and the while also causes the deterioration of natural ecological environment.And fertilizer has the irreplaceable special role of chemical fertilizer, it is the essential condition of Sustainable Agricultural high and stable yields.It contains abundant organic matter, can improve water conservation, fertilizer conservation and the ventilation capacity of soil, and therefore, in the process of Agriculture Production, organic fertilizer plays an important role always.
Biological organic fertilizer one be solid waste take feces of livestock and poultry, stalk, agricultural byproducts and food-processing as raw material, the fermented bacterium fermentation that is equipped with single or several functions processes, the organic fertilizer that contains a certain amount of functional microbial.It is neither traditional fertilizer, and microbial-bacterial fertilizer that neither be simple, is the organic combination of the two, can effectively improve the utilization ratio of fertilizer, strengthens crop root vigor and nutrient absorption capability.Therefore, research and development Rational Application bio-organic fertilizer are not only one of important measures that obtain high grade and yield of crops and increase soil fertility, and also preserve the ecological environment, and promote the inexorable trend of agricultural sustainable development.
But the production of existing biological organic fertilizer all be unable to do without feces of livestock and poultry, because the content of organic matter is very high in feces of livestock and poultry, the nutrient contents such as nitrogen, phosphorus, potassium are also higher, are therefore very suitable for the fertilizer of production high-quality.But, in the ight soil of livestock and poultry, contain a large amount of objectionable impuritiess, the pathogenic micro-organism in ight soil, as: avian influenza virus, Ma Likeshi virus etc., these viral micro-organisms can enter soil and shallow ground water with the water in ight soil, the meeting serious harm environment of prolongation in time; Some growth promoters in animal and fowl fodder simultaneously, anticoccidiosis medicine, disease-resistant medicine can excrete with ight soil, infiltrates in water by surface water or underground water and is drunk by people, cause drug residue and heavy metal poisoning, the water pollution that feces of livestock and poultry produces is thus quite serious.
In addition, chicken manure is untreated to be applied directly in farmland for a long time, by the enrichment that causes phosphorus, copper, zinc and trace element thereof, medicine in soil, thereby to doing deposits yields toxic action, has a strong impact on growing of crop, crop failure; Although carried out the comprehensive development and utilization to feces of livestock and poultry at present, alleviated to a certain extent the harm of feces of livestock and poultry to environment, but along with improving constantly of people's living standard, the demand of meat egg product is being increased, the sterol materials such as oestrogenic hormon in laying hen, dam body, cholesterol are along with ight soil enters in environment, cause the severe contamination of environment, add hormone as the use of the fodder additives pollution of hormone to environment of also having increased weight widely.According to reports, the hormonal activity of steroid substances is much higher than other environmental estrogens class material, and it is mainly derived from the movement of human body and livestock, and these hormones can disturb endocrine dysfunction biological and human normal, cause sex hormone secretion amount and the active and sperm quantity minimizing of organism and human body, the sickness rate such as the abnormal cancer of reproductive organ increase, and reproductive performance is reduced, offspring's health and surviving rate decline, rich long-pending serious threat of the environmental degradation biology causing of steroid and the mankind's persistent existence and procreation.
In addition, it is short that current biological fermentation fertilizer exists the quality guaranteed period, the survival weak effect of effective microbe; And bacterium quantity keeps in microbiological fermented organic fertilizer, the length of the survival time of bacterium is the important indicator of microbial fertilizer, bacterium survival time is long, active strong, accommodative ability of environment and the strong bacterial strain of existence colonization ability are the key problem in technology of producing biological organic fertilizer, are also the basic guarantees of producing biological organic fertilizer on a large scale.
In the biological organic fertilizer of producing at present, contained oestrogenic hormon, drug residue is quite high, environment and people healthy formed to serious threat, how to reduce the impact of this threat environmental pollution, food safety and mankind itself's health, find new having and promote the green micro organism fertilizer of plant growth effect to become problem demanding prompt solution.
Summary of the invention
The present invention is directed to many weak points that existing microbial organic fertilizer exists, a kind of microorganism Fermentation Function compoiste fertilizer and producer thereof are provided, adopt and adopt after the method for mixed fermentation simultaneously, the oestrogenic hormon that can effectively degrade in feces of livestock and poultry, drug residue, can also kill nematode and the fly maggot in soil, and greatly improve the quality guaranteed period of fertilizer and the survival time of viable bacteria, the percentage of germination of raising crop seed and crop anti-adversity etc. simultaneously.
Concrete technical scheme of the present invention is: a kind of production method of microorganism Fermentation Function compoiste fertilizer is as follows:
Below operation is all carried out under aseptic condition,
(1) cultivation of first class inoculum
The weight percentage of the each component of first class inoculum substratum is:
Industry whey powder 2%, industrial Sodium.alpha.-hydroxypropionate 0.4%, industrial anhydrous sodium acetate 0.1%, industrial peptone 1%, bean cake powder 3%, cerelose 1.5%, yeast soak powder 0.5% and water 91.5%, pH value nature;
Concrete operations: adopt 500mL triangular flask, the in-built above substratum 200mL having dissolved of bottle, first add Lactobacillus buchneri Lactobacillus buchneri CICC60040.1mL, add again bacillus cereus Bacillus cereus ACCC101170.1mL, then triangular flask is sealed to mouth with gauze, put into shaking table, 140 turn/per minutes, 37 ± 1 ℃, cultivate 6 hours, stop shaking table, in triangular flask, access baker's active dried yeast Saccharomyces cerevisiae CICC1905 and the each 0.1mL of the husky Lei Shi Serratia marcescent ACCC10119 of cement again, and then triangular flask is sealed, put in shaking table, 140 turn/per minutes, 32 ± 1 ℃, cultivate 10 hours, then stop shaking table, first class inoculum is cultivated and is finished,
(2), seed tank culture
The weight percentage of the each component of seed tank culture base is:
Ammonium sulfate 0.2%, potassium primary phosphate 0.3%, industrial whey powder 1%, industrial Sodium.alpha.-hydroxypropionate 0.5%, industrial anhydrous sodium acetate 0.2%, industrial peptone 1.2%, bean cake powder 4%, cerelose 1.5%, industrial yeast are soaked powder 0.5%, sodium-chlor 0.5%, magnesium sulfate 0.1%, lactose 0.2%, oligochitosan 0.3% and water 89.5%, pH nature;
Concrete operations: 30 ± 1 ℃ of temperature in tank, above cultured first class inoculum is inoculated in tank under aseptic condition, inoculum size is the 2wt% of substratum in tank, sterile air ventilating fermentation, sterile air consumption is: every 0.1 cube of sterile air for 100 kilograms of seed culture fluid per minutes, pH nature, turn/per minute of mixing speed 140, fermentation culture 30 hours, microscopy is qualified without miscellaneous bacteria; Obtain after above-mentioned seed tank culture bacterial classification, utilize this bacterial classification to adopt prior art fermentation to obtain fertilizer.
The present invention also has following technical characterictic:
1, a kind of microorganism Fermentation Function compoiste fertilizer that adopts the production method of a kind of microorganism Fermentation Function compoiste fertilizer as above to make.
2, adopt the production method of a kind of microorganism Fermentation Function compoiste fertilizer as above, or in seed tank culture bacterial classification, add 0.5wt% brown sugar, after dissolving, make foliage fertilizer.
3, a production method for microorganism Fermentation Function compoiste fertilizer, adopts the method for seed tank culture strain fermentation biological organic fertilizer as above, and concrete steps are as follows:
Fill a prescription as follows: take 100kg fertilizer as example, do not comprise water and bacterial classification,
Dried poultrymanure 30kg, dry pig manure 53.4kg, the thin powdered rice hulls 10kg of 40 order, lactose 0.1kg, oligochitosan 0.5kg, wheat bran skin 6kg,
Each material metage adds water 40kg to mix thoroughly after mixing, pile and park 5 hours, then add the cultured seeding tank bacterial classification that accounts for dry material 2wt%, before adding, first water is by weight adding after the dilution proportion of 1: 3, and limit edged turning, starts to stack after adding bacterial classification to mix thoroughly, need to pile 1.5 meters of bottom width, upper wide 1.2 meters, high 1.2 meters, the long long pile of not limitting, the section of buttress is trapezoidal or trilateral, adopts the method for artificial turning or mechanical turning, realizes the aerobic state in stacking;
Turning:
(1) high temperature season: in the time of room temps 30-35 ℃, first day is built heap, turning in second day, and in the 3rd day heap, temperature is 60 ℃ ± 5 ℃, turning in the 4th day, the 5th turning limit, the remotest places add seeding tank bacterial classification in windrow, every cube of seeding tank bacterial classification 3kg for material, and before adding, first water adds after the dilution proportion of 1: 3, then build heap, lid film 36 hours, then removes film and continues fermentation 5 days, with tower dryer, dries and is finished product fertilizer;
(2) the temperature time in: in the time of room temps 10-15 ℃, first day is built heap, turning in the 3rd day, during turning in the 4th day, add seeding tank bacterial classification, every cube of seeding tank bacterial classification 3kg for material, before adding, first water adds after the dilution proportion of 1: 3, then builds heap and covers film after 48 hours, remove film and continue fermentation and will turn over 2-3 time heap in the middle of 8 days, be finished product fertilizer with tower dryer drying and packaging;
(3) the low temperature time: during 0 ℃ of room temps, first day is built heap, turning in the 3rd day, turning in the 6th day, adds seeding tank bacterial classification during turning in the 6th day, every cube of seeding tank bacterial classification 3kg for material, before adding, first water adds after the dilution proportion of 1: 3, then builds heap and covers film, and turning in 96 hours once, altogether need to ferment 15 days, be finished product fertilizer with tower dryer drying and packaging.
In above-mentioned zymotechnique, the microbial strains adopting stable performance during the fermentation, degradation capability is strong, and the reproduction speed of bacterium is fast.The microbial strains of using, is commercially available obtaining.
Wherein adopt after Lactobacillus buchneri Lactobacillus buchneri CICC6004, in the fermenting process of fertilizer, hormonal substance can be hydrolyzed to carbonic acid gas and water, effectively removed the oestrogenic hormon in fertilizer, and select bacillus cereus Bacillus cereus ACCC10117, the effective colistin of mainly degrading of the drug residue (containing in because of feces of livestock and poultry) in degraded fertilizer in the fermenting process of fertilizer, duomycin, can greatly reduce the two content, and the baker's active dried yeast Saccharomyces cerevisiae CICC1905 of the high yield trehalose that contriver adopts, the trehalose producing is discharged in the middle of fertilizer, trehalose itself has the effect of stratification to the seed of crop, trehalose has protection biomass cells and biologically active substance is dewatering, arid, high temperature, freezing, under the unsuitable environmental conditions such as high osmotic pressure, activity is exempted from destroyed function, and to the biological large point of important provide protection of performance such as membrane protein matter and nucleic acid, it is necessary in fermenting organism fertilizer, adding baker's active dried yeast thus.Because of this yeast, can secrete and accumulate a large amount of trehaloses and protect the bacterium of various microorganisms in the middle of fertilizer to live and enzymic activity, extend the quality guaranteed period of fertilizer; Also can improve diastatic activity in crop, the cold-resistant drought-enduring performance significant role through this product trehalose yeast of evidence to farm crop paddy rice, wheat;
The husky Lei Shi Serratia of the cement marcescent ACCC10119 adopting secretes multiple chitinase in the process of fermentation fertilizer, can effectively kill nematode and fly maggot in soil.
In addition, contriver is when seed tank culture bacterial classification, lactose and oligochitosan in formula, have been added respectively, make the required active substance of generation that bacterial classification can be more stable, lactose can be stablized baker's active dried yeast and produce more trehalose, through test of many times, do not add the active dry yeast of lactose to produce trehalose 15% (in dry yeast), and add the active dry yeast of lactose to produce trehalose 25% (in dry yeast); Fecund goes out 10%, has so just greatly improved the result of use of fertilizer, has extended the fertile quality guaranteed period, has reduced production cost, increases economic efficiency; And add after oligochitosan, bacillus cereus degraded fertilizer drug residue, particularly colistin and duomycin have all been degraded more than 90%, have improved 20% with the bacillus cereus phase specific degradation rate that does not add oligochitosan, effect is remarkable, and this is also very big progress compared to prior art.
The various bacterial classifications that adopt, can provide nutrition for soil fungi, can play fixed nitrogen, phosphorus decomposing, potassium decomposing, promotion plant and can produce organic nutrient by forming the relation of mutualism with plant; While fermenting, produce a large amount of organic acids and ferment simultaneously and can improve disease resistance ability, promote plant growth.
In sum, adopt after the method for this mixed fermentation provided by the present invention, the oestrogenic hormon that can effectively degrade in feces of livestock and poultry, drug residue, can also kill nematode and the fly maggot in soil simultaneously, and greatly improved the quality guaranteed period of fertilizer and the survival time of viable bacteria, the percentage of germination of raising crop seed and crop anti-adversity etc., finally obtain a kind of functional green bio fertilizer.
Embodiment
Below in conjunction with embodiment, further illustrate the present invention, can make those skilled in the art more fully understand the present invention, but not limit the present invention in any way.
Embodiment 1:
The cultivation of first class inoculum
The weight percent of the each component of first class inoculum substratum is:
Industry whey powder 2%, industrial Sodium.alpha.-hydroxypropionate 0.4%, industrial anhydrous sodium acetate 0.1%, industrial peptone 1%, bean cake powder 3%, cerelose 1.5%, yeast soaks powder 0.5%, tap water 91.5%, pH value nature, above mix and blend dissolves standby.
Concrete operations: first get 500ml triangular flask, every bottled above substratum 200ml having dissolved, then by 6-8 layer gauze sealing for triangular flask, then with the wrapping of one deck kraft paper, puts into mediclave steam sterilizing, first connect the heating source of sterilizer, when the vapor pressure in sterilizer reaches 0.05 MPa, open the drain tap on sterilizer, discharge freezing air, evacuation time 6-8 minute, when the tensimeter on sterilizer makes zero, close drain tap, continue heating, when the vapor pressure in sterilizer reaches 0.11-0.14 MPa, keep 35 minutes, then sterilizer is left to heating source, allow it naturally cool to 30 ℃ ± 2 ℃ (prior art), then in Bechtop, open triangular flask, in each triangular flask, first add Lactobacillus buchneri Lactobacillus buchneri CICC60040.1mL, add again bacillus cereus Bacillus cereus ACCC101170.1mL, then triangular flask is sealed to mouth with gauze, put into 37 ± 1 ℃ of shaking tables, cultivate 6 hours for 140 rpms, stop shaking table, taking-up triangular flask is put into Bechtop and is opened triangular flask gauze, in triangular flask, access baker's active dried yeast Saccharomyces cerevisiae CICC1905 and the each 0.1mL of the husky Lei Shi Serratia marcescentACCC10119 of cement again, and then triangular flask is sealed, put in shaking table 32 ± 1 ℃, 140 rpms, cultivate 10 hours, then stop shaking table, first class inoculum is cultivated and is finished,
The sterilizing of fermentation equipment pipeline and sterilizing filter
Open each valve of each inlet and outlet piping valve and sterile air pipeline, pass into the steam of 0.12-0.14 MPa, allow steam and pipeline valve UNICOM and have steam discharge, ventilate 10 minutes, then close each drain tap, allow the interior vapor pressure of pipeline keep 0.12-0.14 MPa, keep 40 minutes.
The slack tank sterilizing of seeding tank and fermentor tank
Close each valve, open the sewage draining valve of tank interlayer, then open open steam valve to the steam that passes into 0.12-0.14 MPa in tank, when reaching 121 ± 1 ℃ in tank, start timing, sterilization time is 40 minutes, then closes flushbottom tank valve, and in tank, temperature drops between 25-35 ℃ standby.
Seed tank culture
The weight percent of the each component of seed tank culture base
Ammonium sulfate 0.2%, potassium primary phosphate 0.3%, industrial whey powder 1%, industry Sodium.alpha.-hydroxypropionate 0.5%, industrial anhydrous sodium acetate 0.2%, industrial peptone 1.2%, bean cake powder 4%, cerelose 1.5%, industrial yeast is soaked powder 0.5%, sodium-chlor 0.5%, magnesium sulfate 0.1%, lactose 0.2%, oligochitosan 0.3%, tap water 89.5%, pH nature.
Concrete operations: first the water using in formula is put in the tank of slack tank sterilizing, and start stirrer, then add respectively the starting material in formula, then pass into steam heating, while utilizing jacket steam to be heated to 95 ℃, use again open steam heating instead, when vapor pressure in tank reaches 0.12-0.14 MPa, be incubated 35 minutes, can reach sterilising effect, then steam off, when temperature in tank drops to 30 ± 1 ℃, above cultured first class inoculum is inoculated in tank under aseptic condition, inoculum size is the 2wt% of substratum weight in tank, then build inoculation lid, start sterile air ventilating fermentation, sterile air consumption is 0.1 cube of every 100 kilograms of seed culture fluid per minutes sterile air, pH nature, mixing speed 140 turns per minute, fermentation culture 30 hours, microscopy is qualified without miscellaneous bacteria, more than be called seed tank culture bacterial classification.
Embodiment 2:
The method of utilizing above-mentioned seed tank culture strain fermentation biological organic fertilizer, concrete steps are as follows:
Fill a prescription as follows: take 100kg fertilizer as example, do not comprise water and bacterial classification
Dried poultrymanure 30kg, dry pig manure 53.4kg, the thin powdered rice hulls 10kg of 40 order, lactose 0.1kg, oligochitosan 0.5kg, wheat bran skin 6kg, each material metage adds tap water 40kg to mix thoroughly after mixing, pile and park 5 hours, then add the above cultured seeding tank bacterial classification (2wt% of dry material) of 2wt%, before adding, first with tap water, add after by the dilution proportion of 1: 3, both 1kg bacterium liquid 3kg tap water, limit edged turning, after adding bacterial classification to mix thoroughly, start to stack, need to pile 1.5 meters of bottom width, upper wide 1.2 meters, high 1.2 meters, the long long pile of not limitting.The section of buttress is trapezoidal or trilateral, adopts the method for artificial turning or mechanical turning, realizes the aerobic state in stacking.
Turning: when high temperature season, room temps, 30-35 ℃, first day is built heap, turning in second day, in the 3rd day heap, temperature is 60 ℃ ± 5 ℃, turning in the 4th day, the 5th turning limit, the remotest places are bacterial classification-tap water diluent (adding in addition) of 1: 3 to adding weight ratio in windrow, every cube of pure bacterium liquid 3kg for material, then builds heap, lid film 36 hours, then film is removed and continue fermentation 5 days, with tower dryer, dry and be finished product fertilizer.
In order to adapt to the situation of differing temps, contriver has also determined: the middle temperature time: room temps, in the time of 10-15 ℃, first day is built heap, turning in the 3rd day, and during turning in the 4th day, turning limit, limit is bacterial classification-tap water diluent of 1: 3 to adding weight ratio in heap, pure bacterium liquid 3kg for every square stock, then build heap and cover film after 48 hours, remove film and continue 8 days centres of fermentation and will turn over heap 2-3 time, be finished product fertilizer with tower dryer drying and packaging.
And low temperature season, room temps, 0 ℃ time, first day is built heap, turning in the 3rd day, turning in the 6th day, bacterial classification-tap water diluent that during turning in the 6th day, turning limit, limit weight ratio is 1: 3, pure bacterium liquid 3kg for every square stock, then build heap and cover film, turning in 96 hours once, needs to ferment 15 days altogether, is finished product fertilizer with tower dryer drying and packaging.
The fertilizer that utilizes aforesaid method to obtain has long quality-guarantee period, viable bacteria rate is high, compare with one microbial organic fertilizer, the quality guaranteed period of one microbial organic fertilizer is 6 months, viable bacteria rate keeps 20%, and the biological organic fertilizer quality guaranteed period of utilizing this technology to produce is 12 months, viable bacteria rate keeps 90%, has greatly improved result of use.
The organic fertilizer product index of above-mentioned acquisition is as following table 1:
The quality guaranteed period of one microbial organic fertilizer is 12 months, and viable bacteria rate keeps 80%.
Prior art obtains following (commercially available prod) table 2 of organic fertilizer product index:
The quality guaranteed period of one microbial organic fertilizer is 6 months, and viable bacteria rate keeps 20%.
Add serratia marcescens, nematode and fly maggot death toll synopsis 3:
Visible: by the contrast of his-and-hers watches 1, table 2, the objectionable impurities of table 1 is far below table 2, and this fertilizer oestrogenic hormon and drug residue are low, nematode and the high long quality-guarantee period of fly maggot death toll, effectively viable bacteria content is high.
The fertilizer that utilizes above-described embodiment to obtain carries out field test:
The microbial organic fertilizer (solid) that first every mu of ground used this technology fermentation is as base fertilizer:
1. the liquid microbe fertilizer that utilizes this technology to produce, soaks seed rice by 1: 30 (with tap water dilution, 1kg liquid microbe fertilizer adds tap water 30kg), and soak time is 48 hours, and percentage of germination is compared and improved 55% with control group.Seedling growth is even, and well developed root system is used this liquid microbe bacterial manure foliage spray 1 time for latter 3 days again to blooming after cultivation, every mu of 30 times of 4kg Liquid Fertilizer dilution for ground, weighs during results, compares with control group, with this fertile full seed, every mu is increased production 110 kilograms, paddy rice, has received good benefit.
2. by weight 1: 30 times of diluted liquid fertilizer, soak Wheat Species 2 hours, then sowing, effectively promote the sprouting of wheat seed, improve wheat seed germinating rate, seedling is strong, well developed root system, after wheat flower, within 5 days, press 1: 30 times of foliage spray once, use 3kg Liquid Fertilizer for every mu, results are weighed and are compared with control group, experimental group wheatland wheat does not lodge, growth is even, full seed, and every mu is increased production 85 kilograms.
3. apple thank spend after 5 days by weight 1: 30 times of diluted liquid fertilizer, then carry out foliage spray, young fruit period foliage spray 1 time again, compares with control group, one can increase production 25%, pol is high, resistance to storage can shift to an earlier date listing in 15 days.
4. after 1: 30 times of diluted liquid fertilizer, soak seed of Fructus Cucumidis sativi 8 hours, can promote seed germination, seedling is strong, and 1: the 30 times of diluent of foliage spray in latter 2 days of blooming can improve cucumber yield 25%.
Experimental example 4:
Contriver contrasts the impact of net result after lactose and chitosan are added, and result is as follows:
Add the active dry yeast of lactose to produce the contrast table 4 of trehalose
Add aborning oligochitosan, improved the effect of short lactobacillus degraded hormone, also improved the effect of bacillus cereus degraded drug residue simultaneously.
Estrogenic mensuration effect table 5 in short lactobacillus degraded fertilizer
Add the measurement result table 6 of the strain fermentation fertilizer hormone of oligochitosan
Wax-like gemma bar degraded medicine residue analysis result table 7 in fertilizer
Add the bacillus cereus degraded fertilizer Chinese medicine residue analysis result table 8 of oligochitosan
Visible, the bacterial classification that has added lactose and oligochitosan to cultivate, make the required active substance of generation that bacterial classification can be more stable, lactose energy stabilizing active dry yeast produces more trehalose, through test of many times, do not add the active dry yeast of lactose to produce trehalose 15% (in dry yeast), and add the active dry yeast of lactose to produce trehalose 25% (in dry yeast); Fecund goes out 10%, has so just greatly improved the result of use of fertilizer, has extended the fertile quality guaranteed period, has reduced production cost, increases economic efficiency; And add after chitosan, bacillus cereus degraded fertilizer drug residue, particularly colistin and duomycin have all been degraded more than 90%, have improved 20% with the bacillus cereus degradation rate that does not add oligochitosan, effect is remarkable, and this is also very big progress compared to prior art.
Claims (4)
1. a production method for microorganism Fermentation Function compoiste fertilizer, is characterized in that, method is as follows: following operation is all carried out under aseptic condition,
(1) cultivation of first class inoculum
The weight percentage of the each component of first class inoculum substratum is:
Industry whey powder 2%, industrial Sodium.alpha.-hydroxypropionate 0.4%, industrial anhydrous sodium acetate 0.1%, industrial peptone 1%, bean cake powder 3%, cerelose 1.5%, yeast soak powder 0.5% and water 91.5%, pH value nature;
Concrete operations: adopt 500mL triangular flask, the in-built above substratum 200mL having dissolved of bottle, first adds Lactobacillus buchneri
lactobacillus buchnericICC 6004 0.1mL, then add bacillus cereus
bacillus cereusaCCC10117 0.1mL, then seals mouth by triangular flask with gauze, puts into shaking table, 140 turn/per minutes, and 37 ± 1 ℃, cultivate 6 hours, stop shaking table, then access baker's active dried yeast in triangular flask
saccharomyces cerevisiaethe husky Lei Shi of CICC1905 and cement
serratia marcescentthe each 0.1mL of ACCC 10119, and then triangular flask is sealed, put in shaking table, 140 turn/per minutes, 32 ± 1 ℃, cultivate 10 hours, then stop shaking table, first class inoculum is cultivated and is finished;
(2), seed tank culture
The weight percentage of the each component of seed tank culture base is:
Ammonium sulfate 0.2%, potassium primary phosphate 0.3%, industrial whey powder 1%, industrial Sodium.alpha.-hydroxypropionate 0.5%, industrial anhydrous sodium acetate 0.2%, industrial peptone 1.2%, bean cake powder 4%, cerelose 1.5%, industrial yeast are soaked powder 0.5%, sodium-chlor 0.5%, magnesium sulfate 0.1%, lactose 0.2%, oligochitosan 0.3% and water 89.5%, pH nature;
Concrete operations: 30 ± 1 ℃ of temperature in tank, above cultured first class inoculum is inoculated in tank under aseptic condition, inoculum size is the 2wt% of substratum in tank, sterile air ventilating fermentation, sterile air consumption is: every 0.1 cube of sterile air for 100 kilograms of seed culture fluid per minutes, pH nature, turn/per minute of mixing speed 140, fermentation culture 30 hours, microscopy is qualified without miscellaneous bacteria; Obtain after above-mentioned seed tank culture bacterial classification, utilize this bacterial classification to adopt prior art fermentation to obtain fertilizer.
2. a kind of microorganism Fermentation Function compoiste fertilizer that the production method of a kind of microorganism Fermentation Function compoiste fertilizer according to claim 1 makes.
3. the production method of a kind of microorganism Fermentation Function compoiste fertilizer according to claim 1, is characterized in that: or in seed tank culture bacterial classification, add 0.5wt% brown sugar, after dissolving, make foliage fertilizer.
4. the production method of a kind of microorganism Fermentation Function compoiste fertilizer according to claim 1, is characterized in that, adopts the method for seed tank culture strain fermentation biological organic fertilizer, and concrete steps are as follows:
Concrete formula is as follows: take 100kg fertilizer as example, do not comprise water and bacterial classification,
Dried poultrymanure 30kg, dry pig manure 53.4kg, the thin powdered rice hulls 10kg of 40 order, lactose 0.1kg, oligochitosan 0.5kg, wheat bran skin 6kg,
Each material metage adds water 40kg to mix thoroughly after mixing, pile and park 5 hours, then add the cultured seeding tank bacterial classification that accounts for dry material 2wt%, before adding, first water is by weight adding after the dilution proportion of 1:3, and limit edged turning, starts to stack after adding bacterial classification to mix thoroughly, need to pile 1.5 meters of bottom width, upper wide 1.2 meters, high 1.2 meters, the long long pile of not limitting, the section of buttress is trapezoidal or trilateral, adopts the method for artificial turning or mechanical turning, realizes the aerobic state in stacking;
Turning:
(1) high temperature season: in the time of room temps 30-35 ℃, first day is built heap, turning in second day, and in the 3rd day heap, temperature is 60 ℃ ± 5 ℃, turning in the 4th day, the 5th turning limit, the remotest places add seeding tank bacterial classification in windrow, every cube of seeding tank bacterial classification 3kg for material, and before adding, first water adds after the dilution proportion of 1:3, then build heap, lid film 36 hours, then removes film and continues fermentation 5 days, with tower dryer, dries and is finished product fertilizer;
(2) the temperature time in: in the time of room temps 10-15 ℃, first day is built heap, turning in the 3rd day, during turning in the 4th day, add seeding tank bacterial classification, every cube of seeding tank bacterial classification 3kg for material, before adding, first water adds after the dilution proportion of 1:3, then builds heap and covers film after 48 hours, remove film and continue fermentation and will turn over 2-3 time heap in the middle of 8 days, be finished product fertilizer with tower dryer drying and packaging;
(3) the low temperature time: during 0 ℃ of room temps, first day is built heap, turning in the 3rd day, turning in the 6th day, adds seeding tank bacterial classification during turning in the 6th day, every cube of seeding tank bacterial classification 3kg for material, before adding, first water adds after the dilution proportion of 1:3, then builds heap and covers film, and turning in 96 hours once, altogether need to ferment 15 days, be finished product fertilizer with tower dryer drying and packaging.
Priority Applications (1)
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| CN106520637A (en) * | 2016-12-21 | 2017-03-22 | 中国科学院上海高等研究院 | Compound microbial agent and preparation method and application thereof |
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| CN108046859A (en) * | 2017-12-15 | 2018-05-18 | 北京泰然悦斯莱特农业科技发展有限公司 | The preparation method of organic fertilizer fermentation agent, organic fertilizer and the two |
| CN111793585A (en) * | 2020-08-12 | 2020-10-20 | 杭州新原起生物科技有限公司 | Lactobacillus buchneri, culture method thereof and application thereof in agricultural planting |
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| CN114107128A (en) * | 2021-12-15 | 2022-03-01 | 四川汇邦环保科技有限公司 | Microbial agent for degrading colistin sulfate and application thereof |
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