CN102887743A - Process for producing special bio-organic fertilizer for bananas by taking diatom oozes as additives and product of special bio-organic fertilizer - Google Patents

Process for producing special bio-organic fertilizer for bananas by taking diatom oozes as additives and product of special bio-organic fertilizer Download PDF

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CN102887743A
CN102887743A CN2012104363861A CN201210436386A CN102887743A CN 102887743 A CN102887743 A CN 102887743A CN 2012104363861 A CN2012104363861 A CN 2012104363861A CN 201210436386 A CN201210436386 A CN 201210436386A CN 102887743 A CN102887743 A CN 102887743A
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organic fertilizer
fertilizer
content
banana
bio
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沈其荣
李�荣
黄炎
杨兴明
张苗
施娟娟
曹亮亮
刘红军
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JIANGSU XINTIANDI BIO-FERTILIZER ENGINEERING CENTER CO LTD
Nanjing Agricultural University
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JIANGSU XINTIANDI BIO-FERTILIZER ENGINEERING CENTER CO LTD
Nanjing Agricultural University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention provides a process for producing a special bio-organic fertilizer for bananas by taking diatom oozes as additives and a product of the special bio-organic fertilizer, belonging to the agricultural intensive production technology. The used formulas are decomposed ordinary chicken manure fertilizers (93% dry weight by weight ratio, the same below) and diatom oozes dredged from the lake Taihu (7%). The used strain is the special growth-promoting biocontrol bacillus NJN-6 for bananas. The process flows are as follows: mixing the raw materials according to the optimal formula, inoculating 5% of functional bacteria, carrying out windrow fermentation, carrying out turning once every day and carrying out fermentation for seven days. For the produced fertilizer, the beneficial viable count is more than 2*10<7>CFU/g (dry weight of the fertilizer, the same below), the total nitrogen content is 2-3%, the total nitrogen-phosphorus-potassium nutrient is 5-7%, the organic content exceeds 45% and the contents of microcystins LR and RR are lower than 4mu g/kg. The fertilizer is rich in vast beneficial amino acids and has good growth-promoting effect and strong disease resistance.

Description

Technique take algae mud as Additive Production banana bio-organic fertilizer special and products thereof
One, technical field
The invention provides a kind of technique take algae mud as Additive Production banana bio-organic fertilizer special and products thereof, belong to the intensive agriculture production technology, this biological organic fertilizer is rich in the function yeast of a large amount of promotion Banana Growths, contain simultaneously a large amount of total free aminoacidss, can significantly promote the generation of Banana Growth, prevention and control blight.
Two, background technology
Along with the constant growth of productivity, the improving constantly of people's living standard, a large amount of trade effluents and sanitary sewage enter in river, lake and the reservoir, and the nutritive substance that enters water body constantly increases, and causes the eutrophication of water body in lake to be on the rise.Body eutrophication can cause the explosive breeding of the big area of blue-green algae.The amount reproduction of blue-green algae not only can suppress the growth of other algae, and the transparency of water body is descended, and also can discharge poisonous metabolic substd to water body, and after a large amount of blue-green algae death, can cause the dissolved oxygen wretched insufficiency of water body, produces stench.Consisted of serious threat for ecotope and human health.The method of removing blue-green algae is a lot, directly removes blue-green algae in the water body, and shifts out water body, namely salvages the blue-green algae method, and this is that very effective and academia does not have controversial a kind of method at present.Behind the blue algae bloom of Taihu Lake, directly salvage blue-green algae as emergency schedule, have every day more than 1000 ton of blue algae water from the water body of Taihu Lake, to salvage, approximately 180,000 tons of accumulative totals.Processing after the salvaging of blue-green algae and the salvaging is a large problem demanding prompt solution in the present stage Taihu Lake governance process.But blue-green algae has obvious detrimentally affect to domestic animal, poultry growth, causes the sex change of animal liver, kidney, should not directly utilize.In today of energy shortage, environmental pollution, discarded biomass energy is converted into the available chemical energy source of people, more and more become the focus that people pay close attention to.So both can solve problem of environmental pollution, again can alleviating energy crisis.Blue-green algae mainly contains microcystic aeruginosa, Anabaena Flos-aquae and variation anabena etc., above 3 kinds of blue-green algaes contain abundant nutritive ingredient, there is the expert that the amino acid of these several algaes and the content of lipid acid are measured, and contrasts with other algae (green alga, hidden algae, Euglena and diatom).The aminoacids content of finding three kinds of blue-green algaes is all higher than other algae, wherein all the highest with leucine content in Microcystis aeruginosa and the anabena.Salvage the water content of algae mud between 86-89%, the full N content of algae mud dry-matter is 7.5-8.5%, and full P content is 0.44%, and full K content is 0.5%; Organic content is greater than 70%.(1, Shen Yinwu, Liu Yongding, Wu Guoqiao, etc. the mechanical removal [J] of eutrophic lake Dian Chi bloom blue algae. the hydrobiont journal, 2004,25 (2): research Southern Yangtze University Master's thesis 2009 page 2 of 131-136.2, the quick aerobic compost of blue-green algae).But raw material blue-green algae the inside contains the macromolecular substance such as toxin, albumen, without processing meeting secondary pollution environment (nitrogen, phosphorus, algae toxin), and nutrient can not be directly by plants utilization (Du Jing, " research of Taihu Lake bloom blue algae resource technology " [D], Agricultural University Of Nanjing, 2008, master thesis; Han Zhiguo, Wu Baowang, Zheng Xiesheng, etc. the cyanophycean toxin progress (summary) [J] in the poisons in freshwater. Ji'nan University's journal: natural science edition, 2001,22 (3): 129-134; Yang Hailin, Li Kelang, Zhang Ling, etc. the research [J] of blue algae resource innoxious use technology. biotechnology, 2008,18 (6): 95-98).
Therefore imperative to the wasted resources of salvaging algae mud in recent years.Salvage in the algae mud and contain the abundant nutrition compositions such as vegetable-protein, polysaccharide, this patent is salvaged the amino acid that enriches that algae mud is rich in from Taihu Lake, utilize the short living microorganism of such protein resource and common Chicken dung compost secondary solid fermentation banana, developed the special-purpose short biological organic fertilizer of giving birth to of banana.
Biological organic fertilizer refer to microorganisms with specific functions with mainly take plant and animal residues (such as feces of livestock and poultry, agricultural crop straw etc.) for originating and having the fertilizer of microbial fertilizer and fertilizer effect through the class that harmless treatment, the organic materials that becomes thoroughly decomposed are composited concurrently.Its essential characteristic is to contain microorganism specific function, that show certain fertilizer effect, and the vital movement of these functional microorganisms is key factors that biological organic fertilizer is better than other fertilizer.Can produce in the various active substance dissolves soil insoluble compound after biological organic fertilizer is used and absorb nutrient to increase soil fertility and to be conducive to farm crop; Producing antibacterial substance and growth regulator reduces the generation of crop soil-borne disease insect pest and promotes that crop steadily and surely grows.Therefore the key that biological organic fertilizer plays a role is beneficial microorganism quantity in the fertilizer, studies different prescriptions and secondary solid zymotechnique and guarantees that viable count reaches the highest in the fertilizer and become particularly important.Simply with functional microorganism and plant and animal residues (such as feces of livestock and poultry, agricultural crop straw etc.) for the source and carry out mixed stack through harmless treatment, the organic materials that becomes thoroughly decomposed and be difficult to obtain high-quality biological organic fertilizer, early-stage Study shows in plant and animal residues and to add the quantity that a small amount of exogenous amino acid Secondary Fermentation functional microorganism can significantly increase functional microorganism, improves fertilizer quality.The kind of exogenous amino acid is a lot, and such as dish cake, soya-bean cake etc., but this amino acid cost is very high, and especially soya-bean cake is sought the market competitiveness that then can increase substantially biological organic fertilizer with the low-cost amino acid of development of new.
Three, summary of the invention
Technical problem the object of the invention is to salvage algae mud resource for Taihu Lake can't take full advantage of problem with biological organic fertilizer production cost costliness, develop a kind of prescription and technique of novel banana bio-organic fertilizer special, reduce the production cost of biological organic fertilizer, the banana bio-organic fertilizer special of production can significantly promote the generation of Banana Growth, prevention and control blight.
Technical scheme
Take algae mud as Additive Production banana bio-organic fertilizer special technique, comprising:
1) culture presevation number is inoculated into the PDA nutrient solution for the growth-promoting antagonistic bacterium NJN-6 of CGMCC NO.3183, carry out liquid fermenting production, the condition of fermentative production is: pH6.0~7.5,30~35 ℃ of leavening temperature scopes, stirring velocity is 180~300 rev/mins, the fermentation middle and later periods forms gemma, and fermentation time is 48h, makes to contain bacterium or gemma amount 〉=1 * 10 in the fermented liquid 10Individual/ml.Used PDA nutrient solution compound method is, to prepare the 1L substratum as example: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling, add after filtering the 20g common sucrose in the filtrate, be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.
2) Chicken Manure Compost after will becoming thoroughly decomposed and algae mud evenly mix by the dry weight ratio at 9.3: 0.7;
3) the growth-promoting antagonistic bacterium fermented liquid is inoculated in Chicken Manure Compost after becoming thoroughly decomposed and the algae mud mixture and carried out solid fermentation, add function yeast bacterium liquid 50L in the SOLID ORGANIC material per ton, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, ferment and finished afterwards in 7 days, functional bacterial content can reach 2 * 10 7Individual/more than the g, the water content that is no more than under 60 ℃ the condition microbial organic fertilizer in temperature at last is evaporated to below 30%, and packing is dispatched from the factory and is finished product banana bio-organic fertilizer special; Its germination index of Chicken Manure Compost after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=45%, and the organonitrogen quality is 2-3% than content, the water content mass ratio is 25-30%.
With useful viable count 2 * 10 in the microorganism organic fertilizer of above-mentioned Recipe production 7More than the CFU/g (fertilizer dry weight), total nitrogen content is 2-3%, and total nitrogen phosphorus potassium nutrition is 5-7%, organic content 〉=45%, and the content of microcysin LR and RR all is lower than 4ug/kg in this product.
The mentioned microorganism organic fertilizer is exclusively used in the generation that promotes Banana Growth and preventing and controlling banana fusarium wilt.
Beneficial effect the invention provides a kind of prescription and technique of the low-cost banana bio-organic fertilizer special take algae mud as additive.
It is low, easy to use that the banana special microorganism fertilizer that uses this invention to produce has a production cost, shortly gives birth to effective advantage, is fit to banana planting zone big area and uses.The present invention is for preserving the ecological environment, and the protection people's is healthy, and the added value that improves agricultural-food has great importance.
Product is compared with product in the market has following advantage:
1) this patent utilizes Taihu Lake to salvage algae mud and the common fertile conduct prescription of Chicken dung, the special-purpose Biocontrol Bacillus Bacillus of secondary solid fermentation banana amyloliquefaciens NJN-6, development banana bio-organic fertilizer special, this prescription and technique can increase considerably the quantity of functional microorganism in this novel biological fertilizer, decrease the cost of biological organic fertilizer, the simultaneously effect by microorganism, macromole algae albumen in the blue-green algae can be broken down into micromolecular amino acid, can directly utilize for plant, the algae toxin can be greatly reduced, and the product of production has the extremely strong market competitiveness.
2) product utilization Taihu Lake salvaging algae mud of the present invention is solid fermentation raw materials, can reduce the raw materials cost of biological organic fertilizer.Protein content can be up to more than 50% in the algae mud, and amino acid profiles is more complete, and the content of high efficient strain reaches 2 * 10 in the fertilizer 7More than the CFU/g (fertilizer dry weight), surpass national standard 2 * 10 7Individual/g, energy formation advantage group after being manured into soil, and under in soil, surviving.Contain abundant dissolved organic carbon nitrogen and the total free aminoacids of A wide selection of colours and designs in the fertilizer product, can provide very superior growing environment to function yeast, greatly improved the ability that function is grown and brought into play to growth-promoting antagonistic bacterium deciding in soil.
3) content of algae toxin LR and RR (microcystin-LR and microcystin-RR) in the employing liquid chromatography for measuring biological organic fertilizer, the result shows that the content of microcysin LR and RR all is lower than 4ug/kg (fertilizer dry weight), the content of microcysin LR and RR is respectively 3.1mg/kg (algae mud fresh weight) and 62.16mg/kg (algae mud fresh weight) in the pure algae mud of raw material and we record, therefore can be with the content of algae toxin LR and RR below 4ug/kg (fertilizer dry weight) behind the algae mud recycling the art of this patent solid fermentation the bimester of the placement, the standard that does not also have at present algae toxin in the fertilizer both at home and abroad, standard in the underground water is only arranged, be lower than 1ug/kg.
4) test-results shows, after banana is used this product on seedling stage, can significantly promote Banana Growth.
Four, description of drawings
The number change of Fig. 1 different content algae mud and Chicken dung blended solid fermentation NJN-6 bacterial strain
The quantity of NJN-6 bacterial strain in the fermentation of Fig. 2 biological organic fertilizer
Fig. 3 algae mud adds amino acid whose content in the biological organic fertilizer
Fig. 4 pot test effect figure
Five, embodiment
1, the acquisition of algae mud
Salvage the station by the Taihu Lake periphery and provide free, placed algae mud storage pool 2 months.
2, the production of function yeast microbial inoculum
With culture presevation number be CGMCC NO.3183 growth-promoting antagonistic bacterium NJN-6 (public, see and authorize patent of invention ZL200910183361.3, prevent and kill off Antagonistic Fungi and the microorganism organic fertilizer thereof of continuous cropping banana Panama wilt disease.) be inoculated into the PDA nutrient solution and carry out liquid fermenting production, fermentation condition is: pH6.0-7.0,30-35 ℃ of leavening temperature scope, stirring velocity are 180-300 rev/min, fermentation time is 48 hours, fermented liquid contain bacterium or gemma amount 〉=10 10Individual/ml.Used PDA nutrient solution compound method is, to prepare the 1L substratum as example: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling, add after filtering the 20g common sucrose in the filtrate, be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.
3, solid fermentation generates biological organic fertilizer
3.1 the acquisition of solid fermentation prescription
At first algae mud is added in the Chicken dung compost with different gradients, carry out solid fermentation, add function yeast bacterium liquid 50L in the SOLID ORGANIC material per ton, turning every day is 1 time in the solid fermentation process, makes the solid fermentation temperature be no more than 50 ℃, generally ferments and finishes afterwards in 7 days.The quantity of the function yeast in each processing of the rear mensuration of having fermented, the result shows that such as figure the compost that adds 7% (dry weight) algae mud is the optimum handling in the algae mud gradient, function yeast quantity reaches 6.7 * 10 7CFU/g (dry weight) (Fig. 1).Its germination index of Chicken Manure Compost after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=45%, and the organonitrogen quality is 2-3% than content, the water content mass ratio is 25-30%.Carry out the detection of residual algae toxin, method is as follows: add the acetic acid of 100mL5% in the 100g fertilizer ,-70 and normal temperature under multigelation repeatedly after, the centrifugal 10min of 12000r/min takes out respectively supernatant liquor (I) and precipitation; To precipitate with the concussion of 100mL80% methanol solution and extract 4h, centrifugal 15min gets supernatant liquor (II); With supernatant liquor (I) with after supernatant liquor (II) fully mixes supernatant liquor (III), supernatant liquor (III) is transferred to 3 with dilute hydrochloric acid with pH, centrifugal after the standing over night, abandon residue, get supernatant liquor (IV), after supernatant liquor (IV) transferred to 7 with weak ammonia with pH, 40 ℃ of rotary evaporations were removed methyl alcohol, remaining sample is crossed 0.45 μ m filter membrane get algae toxin crude extract.Use successively 20mL100% methyl alcohol, 20mL ultrapure water activation C18 solid-phase extraction column (500mg/6ml), algae toxin crude extract is carried out enrichment by solid-phase extraction column concentrated, use respectively again 20mL10% and 20ml20% methanol solution drip washing stationary phase, the Microcystin of using at last 10mL80% methanol solution (adding 0.05% trifluoroacetic acid) will be adsorbed on the stationary phase elutes, Microcystin behind the purifying is removed methyl alcohol by rotary evaporation get the MCs storing solution, after the storing solution lyophilize, to 2ml, liquid is for the upper machine testing of HPLC with 20% methanol constant volume.
The content of algae toxin LR and RR (microcystin-LR and microcystin-RR) in the employing liquid chromatography for measuring biological organic fertilizer, the result shows that the content of microcysin LR and RR all is lower than 4ug/kg (fertilizer dry weight), the content of microcysin LR and RR is respectively 3.1mg/kg (algae mud fresh weight) and 62.16mg/kg (algae mud fresh weight) in the pure algae mud of raw material and we record, therefore can be with the content of algae toxin LR and RR below 4ug/kg (fertilizer dry weight) behind the algae mud recycling the art of this patent solid fermentation the bimester of the placement, the standard that does not also have at present algae toxin in the fertilizer both at home and abroad, standard in the underground water is only arranged, be lower than 1ug/kg.
3.2 the fermentation of solid raft generates the finished product fertilizer
According to above-mentioned composition and engineering, algae mud (dry weight ratio is salvaged in Chicken Manure Compost after will becoming thoroughly decomposed and Taihu Lake, 9.3: 0.7) evenly mix, with growth-promoting antagonistic bacterium NJN-6 access hybrid reactor, add function yeast bacterium liquid 50L in the SOLID ORGANIC material per ton and carry out solid fermentation, middle turning every day 1 time makes the solid fermentation temperature be no more than 50 ℃, generally ferments to finish afterwards in 7 days; Design chicken manure decomposed manure in contrast.After the fermentation ends in the novel biological fertilizer function yeast quantity reach 8.2 * 10 7CFU/g (dry weight) (Fig. 2) surpasses 1 order of magnitude of chicken manure decomposed manure.Its germination index of Chicken Manure Compost after used the becoming thoroughly decomposed is more than 98%, and quality of organic matter ratio content 〉=45%, organonitrogen mass ratio are 2-3%, water content mass ratio 25-30%.The novel banana bio-organic fertilizer special that is developed into contains 8.0 * 10 7The growth-promoting antagonistic bacterium NJN-6 that CFU/g (dry weight) is above, total nitrogen content is 2.0-3.0%, and total nitrogen phosphorus potassium nutrition is 5-7%, and organic content is greater than 45%.Above result show the composition and engineering of developing fully can maximized fermentative production novel biological fertilizer, and this biological organic fertilizer can meet national standard fully.
3.3 the aminoacids content in the biological organic fertilizer and composition
Utilize ninhydrin colorimetry to measure the content of the low-cost bio fertilizer Free Amino Acids of developing, the results are shown in Figure 3, after fermentation was finished, the content (7.9g/kg) of novel biological fertilizer (BIO) Free Amino Acids directly added the content (2.2g/kg) of fertilizer (OF) Free Amino Acids that growth-promoting antagonistic bacterium strain solid fermentation forms considerably beyond the contrast chicken manure.
4, short the giving birth in greenhouse tested
Establish altogether 4 processing, the novel short living biological organic fertilizer that is respectively this patent development processes (BIO), the Chicken Manure Compost after becoming thoroughly decomposed is processed (OF), fertilizer is processed the not fertilizer processing (OFL) behind the connection function bacteria solid fermentation of (OFJ) and biological organic fertilizer formula behind the Chicken Manure Compost access function bacteria solid fermentation after becoming thoroughly decomposed.Each processes 18 basins, every basin dress 10kg soil, and the addition of fertilizer is 2%.Fertilizer and soil fully mix, Routine Management.Results of regular determination Banana Growth index.Result such as Fig. 4, table one, table two, table three, table four, table five, table six, table seven, table eight and table nine.1) plant height: compare with the OF processing, OFJ processes the plant height that can increase plant, but difference is not remarkable, behind the interpolation algae mud, can significantly increase the plant height of plant in chicken manure.Compare with other three processing, the application of the biological organic fertilizer BIO of this patent development can significantly increase the plant height of plant.2) stem is thick: process with OF and compare, it is thick that OFJ processes the stem that can increase plant, front 30 days significant differences, and behind the interpolation algae mud, the stem that can significantly increase plant is thick in chicken manure.Process with OF and OFJ and to compare, the stem that the application of the biological organic fertilizer BIO of this patent development can significantly increase plant is thick, but to process difference not remarkable with OFL.3) fall three leaf leaf areas: process with OF and compare, OFJ processes the three leaf leaf areas of falling that can increase plant, but difference is not remarkable, in chicken manure, add algae mud after, can significantly increase the three leaf leaf areas of falling of plant, significant difference.Process with OF and OFJ and to compare, the stem that the application of the biological organic fertilizer BIO of this patent development can significantly increase plant is thick, compares front 30 days significant differences but process with OFL.4) the chlorophyll content difference of three leaf chlorophyll content: OF and OFJ processing is little.Process with OF and OFJ and to compare, OFL processes and the application of the biological organic fertilizer BIO that this patent is developed can significantly increase the chlorophyll content of plant, significant difference.5) blade quantity: compare with the OF processing, OFJ processes can significantly increase blade quantity, significant difference.Process with OF and OFJ and to compare, OFL processes and the application of the biological organic fertilizer BIO that this patent is developed can significantly increase the blade content of plant, significant difference.6) overground part fresh weight and dry weight: process with OF and to compare, OFJ processes can overground part fresh weight and dry weight, but does not reach significant difference.Process with OF and OFJ and to compare, OFL processes and the application of the biological organic fertilizer BIO that this patent is developed can significantly increase overground part fresh weight and the dry weight of plant, significant difference.Compare with the OFL processing, after the application of the biological organic fertilizer of this patent development, the difference of overground part fresh weight reaches significantly, and the difference of dry weight reached remarkable at the 50th day.7) difference was little between underground part fresh weight and dry weight: OF and OFJ processed.Process with OF and OFJ and to compare, OFL processes and the application of the biological organic fertilizer BIO that this patent is developed can significantly increase underground part fresh weight and the dry weight of plant, significant difference.Process with OFL and to compare, the application of the biological organic fertilizer BIO of this patent development, the difference of underground part fresh weight and dry weight is remarkable when the 20th day and 40 days.
Test-results integral body shows, the application of function stem can promote the growth of banana plant, the composition and engineering of this research is the secondary solid fermentation efficiency of promotion functions bacterium significantly, increases the quantity of function yeast in the fertilizer and the nutrient of fertilizer, finally promotes the growth of banana plant.
Table one different fertilizer is for the unit that affects of plant plant height: cm
Table two different fertilizer for plants stems thick affect unit: mm
Figure BSA00000800276100072
Table three different fertilizer affects unit: cm for what plant fell three leaf areas 2
Table four different fertilizer is for the impact of three leaf chlorophyll contents of plant
Figure BSA00000800276100074
Figure BSA00000800276100081
Table five different fertilizer is for the impact of plant leaf quantity
Figure BSA00000800276100082
Table six different fertilizer is for the impact (unit: g) of plant overground part fresh weight
Figure BSA00000800276100083
Table seven different fertilizer is for the impact (unit: g) of plant overground part dry weight
Figure BSA00000800276100084
Table eight different fertilizer is for the impact (unit: g) of plant underground part fresh weight
Figure BSA00000800276100085
Table nine different fertilizer is for the impact (unit: g) of plant underground part dry weight
Figure BSA00000800276100086

Claims (4)

1. take algae mud as Additive Production banana bio-organic fertilizer special technique, comprising:
1) culture presevation number is inoculated into the PDA nutrient solution for the special-purpose short antagonistic Bacillus NJN-6 that gives birth to of the banana of CGMCC NO.3183, carry out liquid fermenting production, the condition of fermentative production is: pH6.0~7.5,30~35 ℃ of leavening temperature scopes, stirring velocity is 180~300 rev/mins, the fermentation middle and later periods forms gemma, and fermentation time is 48h, makes to contain bacterium or gemma amount 〉=1 * 10 in the fermented liquid 10Individual/ml; Used PDA nutrient solution compound method is, to prepare the 1L substratum as example: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling, add after filtering the 20g common sucrose in the filtrate, be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min;
2) Chicken Manure Compost and the algae mud after will becoming thoroughly decomposed evenly mixes than 9.3: 0.7 by dry weight;
3) the growth-promoting antagonistic bacterium fermented liquid is inoculated in Chicken Manure Compost after becoming thoroughly decomposed and the algae mud mixture and carried out solid fermentation, add function yeast bacterium liquid 50L in the SOLID ORGANIC material per ton, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, ferment and finished afterwards in 7 days, functional bacterial content can reach 2 * 10 7Individual/more than the g, the water content that is no more than under 60 ℃ the condition microbial organic fertilizer in temperature at last is evaporated to below 30%, and packing is dispatched from the factory and is finished product banana bio-organic fertilizer special; Its germination index of Chicken Manure Compost after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=45%, and the organonitrogen quality is 2-3% than content, the water content mass ratio is 25-30%.
2. the banana bio-organic fertilizer special product of the described explained hereafter of claim 1, the content of microcysin LR and RR all is lower than 4ug/kg in this product.
3. banana bio-organic fertilizer special product according to claim 1, function yeast NJN-6 viable count is greater than 2 * 10 in this product 7CFU/g fertilizer dry weight, total nitrogen content are 2.0-3.0%, and total nitrogen phosphorus potassium nutrition is 5-7%, organic content 〉=45%.
4. claim 2 and the 3 described products application in promoting Banana Growth and preventing and controlling banana fusarium wilt.
CN2012104363861A 2012-11-01 2012-11-01 Process for producing special bio-organic fertilizer for bananas by taking diatom oozes as additives and product of special bio-organic fertilizer Pending CN102887743A (en)

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CN104030855A (en) * 2014-07-07 2014-09-10 江苏天成科技集团有限公司 Production process for relay fermentation of novel plant growth promoting rhizobacteria type bio-organic fertilizer
CN104961543A (en) * 2015-06-04 2015-10-07 广西贵港市丰收肥业有限公司 Special organic fertilizer for banana and preparation method thereof
CN106495905A (en) * 2016-11-17 2017-03-15 郑州诚合信息技术有限公司 A kind of fertilizer of preventing and treating banana blight
CN110967305A (en) * 2019-11-25 2020-04-07 重庆工商大学 Test paper box for rapidly detecting compost maturity and using method thereof

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CN104030855A (en) * 2014-07-07 2014-09-10 江苏天成科技集团有限公司 Production process for relay fermentation of novel plant growth promoting rhizobacteria type bio-organic fertilizer
CN104961543A (en) * 2015-06-04 2015-10-07 广西贵港市丰收肥业有限公司 Special organic fertilizer for banana and preparation method thereof
CN106495905A (en) * 2016-11-17 2017-03-15 郑州诚合信息技术有限公司 A kind of fertilizer of preventing and treating banana blight
CN110967305A (en) * 2019-11-25 2020-04-07 重庆工商大学 Test paper box for rapidly detecting compost maturity and using method thereof

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