CN104025903A - Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method - Google Patents
Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method Download PDFInfo
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Abstract
The invention provides a liquid fermentation medium for culturing edible mushroom liquid microbial strains. The liquid fermentation medium for culturing the edible mushroom liquid microbial strains comprises, by weight, 1.5% of glucose, 3% of corn flour, 0.2% of peptone, 2% of soybean meal, 0.1% of MgSO4 7H2O, 40.1% of KH2PO, 10.01% of VB, 0.03% of soybean oil and the balance water. The invention further provides an edible mushroom liquid microbial strain preparation method. The preparation method includes activation of inclining microbial strains, cultivation of first-level liquid microbial strains and liquid fermentation. According to the edible mushroom liquid microbial strain production method, through the schemes such as liquid medium formulation optimization and fermentation condition optimization, the yield of cultivation of the liquid microbial strains can be increased by 32.8% compared with that of bagging cultivation of solid microbial strains, the contamination rate is reduced by 4%, the seed production time is shortened, and the labor cost is reduced.
Description
Technical field
The present invention relates to technical field of bioengineering, particularly a kind of liquid fermentation medium of edible fungi liquid strain and preparation method of liquid spawn of cultivating.
Background technology
Along with the increase of domestic and international market to edible fungus demand, the mushroom industry of China rises with average annual 17% speed.Edible Fungi mode is by traditional family workshop type operation, seasonal pattern of producing, gradually to facility, modernization, anniversary, large-scale production development.The change of the mode of production has driven the exploitation of edible mushroom new technology, new technology, new equipment.In recent years, adopt deep liquid culture technology to prepare edible fungi liquid strain for the production of becoming research and development focus, emerged many liquid fermentation device fabrication producers and many reports that adopts liquid spawn in mushroom, woodear, Asparagus, flat mushroom, grifola frondosus production.Liquid spawn is always applied in edible fungus industrial, anniversary, large-scale production, for reducing production costs, improves the quality of products and has remarkable result.Japan, Korea S, in edible fungus industrial is produced, generally adopt liquid spawn.
Such as, Lingshou County planting edible mushroom area has reached 5,000,000 square metres, 100,000 tons of annual productions, along with the development of planting edible mushroom scale, the requirement of bacterial classification also will increase greatly, need every year 400000 bottles, solid original seed, 2,000 ten thousand bottles of cultivated speciess, and need so a large amount of edible fungus species, and to peasant, produce and caused many difficulties, increased a large amount of costs.And liquid spawn has five large advantages: 1, reduce costs: the liquid spawn expense of each cultivation bag only has 1/5th of solid spawn, and inoculation work efficiency than the high 4-5 of solid vaccination doubly; 2, improve purity: liquid spawn is fast-germination in the sealed environment of integral asepsis, thereby bacterial classification purity is high, guarantee that fruiting is healthy and strong; 3, reduce to pollute: liquid spawn sprouting speed has surpassed miscellaneous bacteria and grown speed, the chance that miscellaneous bacteria is not almost grown, has therefore overcome the technical barrier of living contaminants, has guaranteed product quality; 4, fast-germination liquid spawn has mobile permeability, and after inoculation, each point is sprouted, and speed of production is fast, than solid spawn, can shorten for 1/2 time; 5, remarkable benefit: liquid spawn mycelial growth is vigorous, and cell age is short, and fruiting is neat, quality and output are apparently higher than traditional mode of production, thereby the acquisition benefit of winning the market.
Given this plant situation, fast-developing for edible mushroom, research and development are suitable for the liquid spawn of Edible Fungi, will improve the industrialized level of edible fungus culturing, enhance productivity, increase farmers' income, promote mushroom industry fast-developing, economic and social benefits are obvious.
But there is the problems such as spawn activity is low, pollution rate is high, production is chaotic in edible fungi liquid strain in producing, inoculate and cultivating.
Summary of the invention
The object of the invention is for above-mentioned the deficiencies in the prior art, the edible fungi liquid strain that a kind of spawn activity is high, pollution rate is low production method is provided.
For achieving the above object, the technical solution used in the present invention is as follows:
A liquid fermentation medium for edible fungi liquid strain, comprises according to percentage by weight: glucose 1.5%, corn flour 3%, peptone 0.2%, analysis for soybean powder 2%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
10.01%, soya-bean oil 0.03%, and surplus is water.
Wherein, preferably, described edible mushroom is Pleuotus nebrodensis Quel or flat mushroom-89.Flat mushroom-89 are flat mushroom CCEF_ (89), be the strain excellent selecting from external kind, its anti-polygamy is strong, and output is high, of fine quality, this bacterial strain in Hebei, tens provinces and cities such as Henan, Shandong, Jiangsu, Shaanxi, Shanxi and Beijing, Tianjin promote plantation.
A preparation method for edible fungi liquid strain, comprises the following steps:
1) activation of slant strains: slant strains is put on PDA medium, in 25 ℃ of constant temperature culture 7 days, as first class inoculum;
2) cultivation of level liquid bacterial classification: seed liquid nutrient medium is inserted in 300ml triangular flask, and the loading amount of every bottle is 70～90ml, by the first class inoculum that after seed liquid medium sterilization, inoculation step 1 obtains under gnotobasis, is placed in shaking table and cultivates 7 days;
3) liquid fermentation: fermentation broth claimed in claim 1 is inserted in fermentation tank, by after liquid nutrient medium sterilizing under gnotobasis the liquid spawn of inoculation step 2 gained, inoculum concentration is 8～12% of medium by weight percentage, and stir culture 3 days, makes mycelium.
Wherein, preferably, the seed liquor medium adopting in described step 2 comprises by weight percentage: corn flour 3%, wheat bran 2%, glucose 2%, peptone 0.5%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
110mg/L, soya-bean oil 0.03%.
Wherein, preferably, the inoculum concentration in described step 2 is hundred ratio meters by weight, are 8～12% of medium.
Wherein, preferably, in described step 2, Initial pH is 5.5～6.5, and shaking speed is 200～220r/min.
Wherein, preferably, in described step 3, the condition of liquid fermentation is: temperature is 25 ℃, fermentation tank pressure 0.05MPa, and rotating speed of agitator 160～200rpm, throughput is 0.6 (V/Vmin).
Wherein, preferably, described edible mushroom is Pleuotus nebrodensis Quel or flat mushroom-89.
In liquid fermentation medium, carbon source is the important substance that forms somatic cells, is also the required main energy source of edible mushroom vital movement.The utilization of Pleuotus nebrodensis Quel or flat mushroom-89 pair carbon source is quite extensive, both can utilize the compounded carbons of complicated component, (corn flour, starch etc.), also can utilize the little molecule carbon sources such as monose and disaccharide, as (glucose, sucrose etc.), even can absorb alcohols, as (sorbierite).If single carbon source for liquid culture, considers from mycelia dry weight, monose is that glucose is best, and in compounded carbons, corn flour is best.From application, produce reality, the price of corn flour is well below the price of glucose, thereby the economic benefit of corn flour is far away higher than the economic benefit of glucose.
In Pleuotus nebrodensis Quel or flat mushroom-89 liquid culture, different carbon sources has material impact to mycelial form.Wherein, the medium of compounded carbons can be turned out a large amount of bacterium balls, and bacterium bulb diameter is compared with uniformity, and wherein bacterium bulb diameter minimum is maize powder medium, bacterium bulb diameter average out to 2.1mm, secondly soluble starch, glucose, sucrose.By contrast, what little molecule carbon source medium culture went out take mycelia as many, and bacterium ball is less.Tracing it to its cause, is that nutrient component is abundanter because the composition of compounded carbons is more complicated, and the inside contains more solid material, thereby the viscosity of medium is increased.Due in certain scope, viscosity increases the formation that is conducive to bacterium ball, and too high or too low viscosity is all unfavorable for the Growth and reproduction of bacterium ball.And little molecule carbon source, because it can directly be absorbed by mycelia, even mycelia branch, extension in a short time, mycelial growth becomes greatly feature with chap, thereby in the later stage, occur oligotrophy, and cause the inner anoxic of mycelia, forming larger mycelia fragment has consequently affected the formation of bacterium ball.But observe to find, with little molecules such as glucose, do carbon source, early stage mycelial growth more vigorous, the later stage may decline to some extent because oligotrophy causes biomass.So, compounded carbons and little molecule carbon source are combined to best results.
Carbon source is the basis of fermentation medium, and it is cyto-architectural important composition composition, is again the primary raw material that thalline produces reserve substance in various metabolites and cell.On carbon source is selected, also to consider the impact of carbon source on bacterium nodule number.The bacterium nodule number of compounded carbons is obviously more than little molecule carbon source, and maximum is the bacterium nodule number of maize powder medium, and the poorest is sorbierite; Producing the reason of this result, is mainly that compounded carbons nutrition is abundanter, not only for Pleuotus nebrodensis Quel liquid culture provides carbon source, wherein also contains some growth factor, thereby is more conducive to the growth of Pleuotus nebrodensis Quel.Because bacterium nodule number is many, can reach in unit volume and have the more bacterium point of sending out, be more conducive to the cultivation of next stage.
In compounded carbons, corn flour is being all best carbon source aspect biomass, bacterium bulb diameter and bacterium nodule number, and little molecule is best with glucose.Consider the cost in production, this test is definite take corn flour as compounded carbons, and little molecule be take glucose as carbon source.Because compounded carbons is the nutriment slowly utilizing, and little molecule carbon source has the feature being absorbed by mycelium fast, in order mutually to cover the shortage, reaches the effect of optimum carbon source by add little molecule carbon source in compounded carbons.
Nitrogenous source is edible mushroom cell synthetic protein and the requisite primary raw material of nucleic acid, plays very important effect in submerged fermentation process.From Pleuotus nebrodensis Quel and flat mushroom-89, analysis for soybean powder, peptone, wheat bran, all can well grow, and in urea, ammonium sulfate, potassium nitrate undergrowth, illustrate that Pleuotus nebrodensis Quel is not good to the utilization of inorganic nitrogen, wherein using analysis for soybean powder during as nitrogenous source, mycelial growth amount is the highest, and peptone takes second place.The features such as consider from economic aspect, analysis for soybean powder is as agricultural product convenient sources, with low cost, simultaneously peptone as growth because being conducive to the growth of mycelia.
In level liquid bacterial classification condition of culture, Initial pH mycelia between 5.5-6.5 can grow preferably, and wherein when pH value 6.5, the pregnant amount of growth of bacterium is the highest.When inoculum concentration is less than 10%, along with the increase of inoculum concentration, biomass also increases, but while surpassing 10%, because mycelium production is not subject to the impact of inoculum concentration substantially, therefore determines that 10% inoculum concentration be the best.With the hypha biomass of 80mL liquid amount gained, for the highest, higher than the liquid amount hypha biomass of 80mL, decline to some extent, reason is that liquid amount is many, and throughput is just few, and in medium, dissolved oxygen is also few, cannot meet the needs of thalline vigorous growth, causes under biomass.Shaking speed is too low, makes hypoventilation, thereby makes biomass lower, and when rotating speed is 210r/min, biomass is maximum.
In liquid fermentation, when throughput is 0.6 (V/Vmin), bacterium ball is large, and and many, look orange, and foam situation is comparatively moderate.When mycelium inoculation amount is 8～12%, rapidly, fermentation period is short for the breeding of thalline primary growth, and while being fermentation termination, the accessory substance of generation is less.And observe while cultivating 3d left and right, it is minimum 3.5 that pH drops to, and observes mycelium and find that now mycelial growth is vigorous, and sturdy, mycelium pellet quantity is more.Therefore, pH value is cultivated one of terminal reference index in 3.5 left and right as judgement.
And maximum at fermentation initial stage dissolved oxygen content, along with the mycelia that carries out of fermenting conforms after beginning raised growth gradually, dissolved oxygen starts to decline gradually.
To be finished vigor the highest with interior at 12h for liquid spawn of the present invention, and 10 ℃ of the bests of storage temperature, should be with inoculation in 3 days.
The invention has the beneficial effects as follows: edible fungi liquid strain production method of the present invention, by schemes such as liquid nutrient medium formulation optimization and fermentation condition optimization, can make liquid spawn compared with solid spawn cultivating in bag volume increase 32.8%, pollution rate reduces by 4%, save the production of hybrid seeds time, reduce labor cost.
Embodiment
Below the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making all other embodiment that obtain under creative work prerequisite, belong to the scope of protection of the invention.
A liquid fermentation medium for edible fungi liquid strain, comprises according to percentage by weight: glucose 1.5%, corn flour 3%, peptone 0.2%, analysis for soybean powder 2%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
10.01%, soya-bean oil 0.03%, and surplus is water.
A preparation method for edible fungi liquid strain, comprises the following steps:
1) activation of slant strains: slant strains is put on PDA medium, in 25 ℃ of constant temperature culture 7 days, as first class inoculum;
2) cultivation of level liquid bacterial classification: seed liquid nutrient medium is inserted in 300ml triangular flask, and the loading amount of every bottle is 70～90ml, by the first class inoculum that after seed liquid medium sterilization, inoculation step 1 obtains under gnotobasis, is placed in shaking table and cultivates 7 days;
3) liquid fermentation: fermentation broth claimed in claim 1 is inserted in fermentation tank, by after liquid nutrient medium sterilizing under gnotobasis the liquid spawn of inoculation step 2 gained, inoculum concentration is 8～12% of medium by weight percentage, and stir culture 3 days, makes mycelium.
The making of liquid spawn and the technological process of cultivation thereof: the cultivation-liquid spawn of slant strains-level liquid bacterial classification-receive in planting material and cultivate-cultivation management-fruiting.
For trying plant formulation: corncob 40%, wood chip 40%, corn flour 8%, rice chaff 8%, gypsum 1%, superphosphate 1%, sugar 2%, potassium dihydrogen phosphate 0.2%, moisture content 60%, pH value is 6.5.
By cultivation bag, be 17 * 33cm, the poly-interior alkene plastics bag that thickness is 0.045cm, every sacked material is 400g.Planting material is installed in bag, with forefinger, two angles of bag are pressed in bag, compress composts or fertilisers of cultivating, then continue feed and use compacting, after filling, in the middle of bag, with taper wooden stick, prick a hole, extract wooden stick, object is liquid spawn to be poured into medium the inside.Then by cultivation bag autoclaving, after sterilizing is cooling, under gnotobasis, connect liquid spawn.
Embodiment 1
A preparation method for Pleuotus nebrodensis Quel liquid spawn, comprises the following steps:
1) activation of slant strains: slant strains is put on PDA medium, in 25 ℃ of constant temperature culture 7 days, as first class inoculum;
2) cultivation of level liquid bacterial classification: seed liquid nutrient medium is inserted in 300ml triangular flask, the loading amount of every bottle is 80ml, by the first class inoculum that after seed liquid medium sterilization, inoculation step 1 obtains under gnotobasis, inoculum concentration is hundred ratio meters by weight, for 8～12% of medium, be placed in shaking table and cultivate 7 days; Seed liquid nutrient medium is: corn flour 3%, wheat bran 2%, glucose 2%, peptone 0.5%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
110mg/L, soya-bean oil 0.03%.Initial pH is 5.5, and shaking speed is 210r/min.
3) liquid fermentation: liquid fermentation medium is inserted in fermentation tank, by after liquid nutrient medium sterilizing under gnotobasis the liquid spawn of inoculation step 2 gained, inoculum concentration is 10% of medium by weight percentage, stir culture 3 days, makes mycelium.The condition of liquid fermentation is: temperature is 25 ℃, fermentation tank pressure 0.05MPa, and rotating speed of agitator 180rpm, throughput is 0.6 (V/Vmin).
Liquid seed culture medium: glucose 1.5%, corn flour 3%, peptone 0.2%, analysis for soybean powder 2%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
10.01%, soya-bean oil 0.03%, and surplus is water.
Embodiment 2
A preparation method for Pleuotus nebrodensis Quel liquid spawn, comprises the following steps:
1) activation of slant strains: slant strains is put on PDA medium, in 25 ℃ of constant temperature culture 7 days, as first class inoculum;
2) cultivation of level liquid bacterial classification: seed liquid nutrient medium is inserted in 300ml triangular flask, the loading amount of every bottle is 70ml, by the first class inoculum that after seed liquid medium sterilization, inoculation step 1 obtains under gnotobasis, inoculum concentration is hundred ratio meters by weight, for 12% of medium, be placed in shaking table and cultivate 7 days; Seed liquid nutrient medium is: corn flour 3%, wheat bran 2%, glucose 2%, peptone 0.5%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
110mg/L, soya-bean oil 0.03%.Initial pH is 6.0, and shaking speed is 200r/min.
3) liquid fermentation: liquid fermentation medium is inserted in fermentation tank, by after liquid nutrient medium sterilizing under gnotobasis the liquid spawn of inoculation step 2 gained, inoculum concentration is 8% of medium by weight percentage, stir culture 3 days, makes mycelium.The condition of liquid fermentation is: temperature is 25 ℃, fermentation tank pressure 0.05MPa, and rotating speed of agitator 160rpm, throughput is 0.6 (V/Vmin).
Liquid seed culture medium: glucose 1.5%, corn flour 3%, peptone 0.2%, analysis for soybean powder 2%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
10.01%, soya-bean oil 0.03%, and surplus is water.
Embodiment 3
A preparation method for Pleuotus nebrodensis Quel liquid spawn, comprises the following steps:
1) activation of slant strains: slant strains is put on PDA medium, in 25 ℃ of constant temperature culture 7 days, as first class inoculum;
2) cultivation of level liquid bacterial classification: seed liquid nutrient medium is inserted in 300ml triangular flask, the loading amount of every bottle is 90ml, by the first class inoculum that after seed liquid medium sterilization, inoculation step 1 obtains under gnotobasis, inoculum concentration is hundred ratio meters by weight, for 8% of medium, be placed in shaking table and cultivate 7 days; Seed liquid nutrient medium is: corn flour 3%, wheat bran 2%, glucose 2%, peptone 0.5%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
110mg/L, soya-bean oil 0.03%.Initial pH is 6.5, and shaking speed is 220r/min.
3) liquid fermentation: liquid fermentation medium is inserted in fermentation tank, by after liquid nutrient medium sterilizing under gnotobasis the liquid spawn of inoculation step 2 gained, inoculum concentration is 12% of medium by weight percentage, stir culture 3 days, makes mycelium.The condition of liquid fermentation is: temperature is 25 ℃, fermentation tank pressure 0.05MPa, and rotating speed of agitator 200rpm, throughput is 0.6 (V/Vmin).
Liquid seed culture medium: glucose 1.5%, corn flour 3%, peptone 0.2%, analysis for soybean powder 2%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
10.01%, soya-bean oil 0.03%, and surplus is water.
The Pleuotus nebrodensis Quel liquid spawn quality and technical index of above-described embodiment system is as shown in table 1:
Table 1 Pleuotus nebrodensis Quel liquid spawn quality and technical index
Embodiment 4 Pleuotus nebrodensis Quel liquid spawns and solid spawn bag are planted comparative trial,
Liquid spawn and solid spawn are to output comparison
Test shows, connects the output of liquid spawn, is all not less than the output of solid spawn.When inoculum concentration is 10%, bag average yield reaches 269.3g, and biological efficiency is 67.3%, and the output of solid spawn is 202.7g, and biological efficiency is 50.7%.Concrete condition is in Table 2.
Table 2 liquid spawn and solid spawn bag are planted output comparison (unit: g)
From table 2, be not difficult to find, the fruiting date of liquid spawn shifts to an earlier date than solid spawn, the fastest reaches 40 days in advance, and higher than the output of solid spawn by the output of liquid spawn, its reason may be that liquid spawn is active strong, the germination point that the mobility of liquid makes increases, and mycelia day, long speed was very fast, thereby required time shortens.The increase of its output is because the sprouting of liquid spawn is neat, because the medium list of liquid spawn contains good nutriment, has increased the nutrient component of culture bag list, so liquid spawn is high compared with the output of solid spawn simultaneously.
The comparison of fruit body quality
The commercial quality of Pleuotus nebrodensis Quel is consist of the outward appearance of product and numerous internal factors comprehensive, mainly comprises organoleptic quality, nutritional quality and safety requirements etc.The Sales Channel of Pleuotus nebrodensis Quel is mainly foreign trade, outlet at present, therefore, the requirement of quality is improved gradually the benefit that good quality has just had.From the characteristic of outward appearance, by the length of stem and the diameter of cap, weigh judgement, result is as table 3.
The comparison of the fruit body quality of table 3 liquid spawn and solid spawn
Pleuotus nebrodensis Quel liquid strain cultivation entity quality close with solid spawn.Pleuotus nebrodensis Quel color and luster is pure white, and mushroom matter is tender and crisp, is hemispherical when cap is immature, and edge is involute, after grow up gradually open and flat.By table 3, can find out, liquid spawn the stem of entity longer, cap is greater than solid spawn.Under identical management condition, the reason of its difference may be that the nutrition of liquid spawn itself is slightly better than solid spawn, so length is more sturdy.
The performance analysis of liquid strain cultivation
Liquid spawn, as a kind of new technology, has good economic benefit, and economic benefit is to calculate by the input of the various means of production (labour, machinery, fertilizer, agricultural chemicals, seed, the energy etc.) and the output of use value.Relation in this production between input and output, essence is after material production resource devotes certain agricultural production, under people's work and the promotion of mechanization power, a kind of mass exchange relation producing by means of various elemental effects.This material relation, by the conversion of the magnitude of value, is reflected as the economic relation between cost consumption and product income.Simply, be exactly economic benefit=gained-institute expense.High being of economic benefit of liquid spawn produced edible mushroom with it, and production can be carried out according to high yield, high-quality, low consumed requirement.Therefore, according to cultivation research and test data above, carried out the benefit comparison of liquid spawn and solid spawn, result is as table 4.
The every thousand bag liquid bacterial classifications of table 4 Pleuotus nebrodensis Quel and solid spawn production cost comparison (unit: unit)
| Project | Liquid spawn produces | Solid spawn produces |
| Raw material cost | 350 | 350 |
| Labour cost | 460 | 630 |
| Charges for water and electricity | 90 | 40 |
| Bacterial classification takes | 15 | 170 |
| Implement depreciation cost | 120 | 50 |
| Other expenditure | 100 | 100 |
| Total expense | 1135 | 1340 |
| Average bag output (g) | 269.3 | 202.7 |
| Market price | 15 | 15 |
The economic benefit Y of every thousand bags that can calculate Pleuotus nebrodensis Quel liquid spawn by table is:
Y=15 * 269.3/1000 * 1000-1135=2904.5 (unit);
The economic benefit Y of solid spawn is: Y=15X202.7/1000X1000-1340=1700.5 (unit),
This is with batch different difference of production gained, can find out by the Pleuotus nebrodensis Quel benefit that liquid spawn produces and can have more 1204 yuan than solid spawn.Thereby, can show that the production of liquid spawn has a good application prospect.
Reducing costs the benefit of generation: adopt liquid spawn, the shared cost of each cultivation bag bacterial classification is 0.02 yuan, is 0.17 yuan and adopt solid spawn.The pollution rate that employing solid spawn produces, all in 6% left and right, is 2% left and right and adopt liquid spawn to pollute, and yield rate improves 4 white branches, can increase by 116.2 yuan/1000 bags of net incomes.So, adopting liquid spawn, the benefit that reduces costs generation can reach 5～8%.
The benefit that the shortening time produces: adopt liquid strain cultivation Pleuotus nebrodensis Quel, the production of hybrid seeds time of traditional solid spawn is shortened to 30 days by 3 wheat harvesting periods, the time that cultivation bag is sent out bacterium is only in the past half.Due to the shortening of liquid spawn production cycle, cultivation family can be transformed existing facility, just can carry out entire-year standardized production, and product can go on the market in advance, and yes is doubled and redoubled for its benefit.
Improve the benefit that output produces: through test of many times, adopt every bag of output of liquid strain cultivation Pleuotus nebrodensis Quel to improve 32.8% left and right.
Reduce the benefit that recruitment produces: solid spawn produces spice, bottling (bag), sterilizing, inoculation, bacteria, complicated operation, labour intensity is large.And adopt liquid spawn not only can save these production links, and order also improves 2～3 times than solid efficiency in inoculation operation.Comprehensive above various factors, a small-sized cultivation family adopts the comparable employing solid spawn of liquid spawn to reduce more than 50 of recruitments, by 20 yuan of works, just can save 1000 yuan.
Above analysis result fully proves, liquid spawn has significant economic benefit and social benefit.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. a liquid fermentation medium for edible fungi liquid strain, is characterized in that, according to percentage by weight, contains: glucose 1.5%, corn flour 3%, peptone 0.2%, analysis for soybean powder 2%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
10.01%, soya-bean oil 0.03%, and surplus is water.
2. the liquid fermentation medium of a kind of edible fungi liquid strain according to claim 1, is characterized in that: described edible mushroom is Pleuotus nebrodensis Quel or flat mushroom-89.
3. a preparation method for edible fungi liquid strain, is characterized in that, comprises the following steps:
1) activation of slant strains: slant strains is put on PDA medium, in 25 ℃ of constant temperature culture 7 days, as first class inoculum;
2) cultivation of level liquid bacterial classification: seed liquid nutrient medium is inserted in 300ml triangular flask, and the loading amount of every bottle is 70～90ml, by the first class inoculum that after seed liquid medium sterilization, inoculation step 1 obtains under gnotobasis, is placed in shaking table and cultivates 7 days;
3) liquid fermentation: liquid fermentation medium claimed in claim 1 is inserted in fermentation tank, by after liquid nutrient medium sterilizing under gnotobasis the liquid spawn of inoculation step 2 gained, inoculum concentration is 8～12% of medium by weight percentage, and stir culture 3～7 days, makes mycelium.
4. the preparation method of a kind of edible fungi liquid strain according to claim 3, is characterized in that, the seed liquor medium adopting in described step 2 contains by weight percentage: corn flour 3%, wheat bran 2%, glucose 2%, peptone 0.5%, MgSO
47H
2o0.1%, KH
2pO
40.1%, VB
110mg/L, soya-bean oil 0.03%.
5. the preparation method of a kind of edible fungi liquid strain according to claim 3, is characterized in that, the inoculum concentration in described step 2 is hundred ratio meters by weight, are 8～12% of medium.
6. the preparation method of a kind of edible fungi liquid strain according to claim 3, is characterized in that, in described step 2, Initial pH is 5.5～6.5, and shaking speed is 200～220r/min.
7. the preparation method of a kind of edible fungi liquid strain according to claim 3, it is characterized in that, in described step 3, the condition of liquid fermentation is: temperature is 25 ℃, fermentation tank pressure 0.05MPa, rotating speed of agitator 160～200rpm, throughput is 0.6 (V/Vmin).
8. according to the preparation method of a kind of edible fungi liquid strain described in claim 3～7, it is characterized in that, described edible mushroom is Pleuotus nebrodensis Quel or flat mushroom-89.
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| CN106497798A (en) * | 2016-10-27 | 2017-03-15 | 稼圃麟（廊坊）生物科技发展有限公司 | Edible fungus species are carried out with fluid medium and the propagation method of rapid expansion breeding |
| CN106754414A (en) * | 2016-12-16 | 2017-05-31 | 中国科学院天津工业生物技术研究所 | A kind of siberian Ginseng P.E induction fermentation black fungus bacterial and its mycelia preparation |
| CN106993465A (en) * | 2016-01-22 | 2017-08-01 | 许昌世纪香生物科技有限公司 | Pleurotus nebrodensis liquid spawn is cultivated and Pleurotus nebrodensis bottle plants industrial production method |
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| CN115812946A (en) * | 2023-01-05 | 2023-03-21 | 南京师范大学 | Method for preparing edible fungus mushroom sauce |
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| CN104782384A (en) * | 2015-03-19 | 2015-07-22 | 黄秀英 | Method for recovering ganoderma lucidum solid strain into liquid strain |
| CN104756767B (en) * | 2015-04-29 | 2016-11-30 | 天津农学院 | A kind of high density pleurotus eryngii liquid strain and fermentation process thereof |
| CN104756767A (en) * | 2015-04-29 | 2015-07-08 | 天津农学院 | High-density pleurotus eryngii liquid strain and fermentation method thereof |
| CN106993465A (en) * | 2016-01-22 | 2017-08-01 | 许昌世纪香生物科技有限公司 | Pleurotus nebrodensis liquid spawn is cultivated and Pleurotus nebrodensis bottle plants industrial production method |
| CN106497798B (en) * | 2016-10-27 | 2018-03-16 | 稼圃麟（廊坊）生物科技发展有限公司 | Edible fungus species are carried out with the fluid nutrient medium and propagation method of rapid expansion breeding |
| CN106497798A (en) * | 2016-10-27 | 2017-03-15 | 稼圃麟（廊坊）生物科技发展有限公司 | Edible fungus species are carried out with fluid medium and the propagation method of rapid expansion breeding |
| CN106754414A (en) * | 2016-12-16 | 2017-05-31 | 中国科学院天津工业生物技术研究所 | A kind of siberian Ginseng P.E induction fermentation black fungus bacterial and its mycelia preparation |
| CN107189948A (en) * | 2017-06-07 | 2017-09-22 | 河南省农业科学院植物营养与资源环境研究所 | A kind of culture medium and cultural method of promotion flat mushroom liquid mycelia fast-growth |
| CN107641601A (en) * | 2017-10-20 | 2018-01-30 | 翔天农业开发集团股份有限公司 | A kind of liquid fermentation strain domestication method |
| CN108718915A (en) * | 2018-04-25 | 2018-11-02 | 广西壮族自治区农业科学院微生物研究所 | Improve the culture medium and cultural method of pleurotus edible fungus yield |
| CN108849240A (en) * | 2018-07-10 | 2018-11-23 | 山东省农业科学院农业资源与环境研究所 | A kind of edible fungi liquid strain precision fermentation medium and application |
| CN111034539A (en) * | 2018-10-15 | 2020-04-21 | 于智勇 | Liquid culture medium for edible fungi |
| CN110583371A (en) * | 2019-09-23 | 2019-12-20 | 贵州同辉食用菌发展有限公司 | Fermentation method of mushroom liquid strain with high activity and high stability |
| CN113812302A (en) * | 2021-10-11 | 2021-12-21 | 中国农业科学院农业资源与农业区划研究所 | Method for preparing liquid edible fungus strain |
| CN115812946A (en) * | 2023-01-05 | 2023-03-21 | 南京师范大学 | Method for preparing edible fungus mushroom sauce |
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