CN102630483A - Method for shortening cultivation period of needle mushroom during production - Google Patents
Method for shortening cultivation period of needle mushroom during production Download PDFInfo
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- CN102630483A CN102630483A CN2012101122878A CN201210112287A CN102630483A CN 102630483 A CN102630483 A CN 102630483A CN 2012101122878 A CN2012101122878 A CN 2012101122878A CN 201210112287 A CN201210112287 A CN 201210112287A CN 102630483 A CN102630483 A CN 102630483A
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Abstract
The invention relates to the field of new edible mushroom cultivation techniques, in particular to a method for shortening a cultivation period of needle mushroom during production. The method comprises the following steps of: with grains as a main raw material, preparing a culture matrix, wherein the grains are rich in nutrition, the culture matrix made of the grains is hardened so that inoculated strains can be easily dispersed and can penetrate into the culture matrix; inoculating by utilizing liquid strains, wherein the cultivation period of the liquid strains is short, the inoculation operation is convenient and fast; and carrying out shaking, clamping and other measures on the culture matrix in a bottle after inoculating so that the inoculated liquid strains are in full contact with the culture matrix in the bottle and cultivated hypha can fully grow in the bottle after 10-20 days. Through the embodiment of the invention, the whole period of the preparation of the mushroom culture matrix only need 25-27 days, but the whole period of the preparation of the mushroom culture matrix by the traditional method needs three months nearly, thus the production efficiency is largely improved; in addition, compared with the traditional method, the method has the advantages that the cultivated needle mushroom is two stubbles more than needle mushroom cultured by the traditional method and the utilization rate of the field is largely improved; and the method disclosed by the invention is more beneficial to industrial, intensive and extensive production of needle mushroom.
Description
Technical field
The present invention relates to a kind of edible fungus culturing new technical field, be specifically related to shorten when a kind of Asparagus is produced the method in its cultivation cycle.
Technical background
Traditional Asparagus production process is divided into into three phases, and the phase I is to make female plant (one-level kind), and the cycle approximately needs 10 days; Second stage is mother's kind to be transferred to related raw material cultivate making original seed (secondary kind), and this stage cultivation cycle approximately needs 25～30 days; Three phases is the original seed for preparing to be transferred to related raw material cultivate and make cultivated species (three grades of kinds), its cycle in this stage approximately need 30～40 days can fruiting.Nearly three months of whole process need, cultivation cycle is long can to increase a lot of costs virtually, simultaneously can be at any time some such as the influence of temperature change fruiting, miss unpredictalbe risk of best sales season or the like and take place, the availability in place is not high yet.
In order to make the plantation Asparagus can produce more considerable benefit, the producer has taked various measures in process of production.Application number is " shortening the method that Asparagus batch production bottle is planted the production cultivation cycle " of 201010254999.4.This invention is a kind of method that Asparagus batch production bottle is planted the production cultivation cycle that shortens; It is characterized in that: Asparagus batch production bottle is planted to produce adopt liquid spawn; The shape of charge level of adjustment composts or fertilisers of cultivating; The adjusting temperature shortens the cultivation of Asparagus bottle and supports the cycle, the concrete operations step: charge level mode smooth in the middle of forming, depression all around is carried out the bottling of Asparagus composts or fertilisers of cultivating after adopting bottling to burrow; Adopt liquid spawn to inoculate; After planting inoculation, cultivates by Asparagus batch production bottle; 17 ℃～19 ℃ in earlier stage of culturing room's temperature; The 2nd～3 day Asparagus mycelia under suitable temperature growth rapidly, the quick front cover of charge level was transferred to Asparagus in 13 ℃～16 ℃ the culturing room after the 5th～6 day; Carry out the later stage cultural hypha, the 17th～20 day full whole material bottle.
This invention is through the shape of charge level of adjustment culture bottle composts or fertilisers of cultivating and inserts liquid spawn, in cultivating the mycelia process, several times changes environmental temperature especially.From the operation of this invention and process, come out, its technology is also quite loaded down with trivial details, influences the operation process to a great extent with seeing; Significantly reduce operating efficiency; Mycelia sends out bottle full also needs 17～20 days, adds and makes female the kind and liquid spawn early stage, and the whole cycle needs 32～35 days.
Summary of the invention
The object of the invention is exactly to overcome the deficiency of prior art and the method that shortens its cultivation cycle when providing brand-new a kind of Asparagus to produce.Specifically comprise: using with grain is that primary raw material prepares culture base-material, and grain is not only nutritious, and the culture base-material of its formation also can not harden, and helps inoculating the back bacterial classification and is prone to disperse and be penetrated in the culture base-material; Applying liquid spawn is inoculated; The strain cultivation cycle is short, inoculate convenient and swift, after the inoculation again through measures such as the culture base-material in the bottle are trembled, buckled; Make the liquid spawn and the interior culture base-material comprehensive engagement of bottle of access, can cover with bottle through 10～12 days cultivation mycelia again; The quality requirement that also comprises flammulina velutipes liquid strains in the implementation process, the cultivating container that is used for fruiting is selected the preparation of mushroom producing culture matrix and management of producing mushroom method etc.
The present invention realizes through following technical scheme:
Shorten the method in its cultivation cycle when 1, Asparagus is produced; Comprise in the technical scheme: using with grain is that primary raw material prepares culture base-material; The quality requirement of flammulina velutipes liquid strains, the cultivating container that is used for fruiting is selected the preparation of mushroom producing culture matrix and management of producing mushroom method.
Shorten the method in its cultivation cycle when 2, Asparagus is produced, use with grain in the technical scheme that to be that primary raw material prepares the method for culture base-material following:
(1) raw material and the proportioning formed: paddy 82%, weed tree sawdust 9%, wheat bran 5%, corn flour 3%, land plaster 1%, water is an amount of;
(2) preparation: earlier place clear water to soak paddy and pull the elimination excessive moisture out after 40～48 hours; Then mix and the resulting mixture that stirs, admix an amount of clear water at last and about 50%, form culture base-material until the water content of mixture with weed tree sawdust, wheat bran, corn flour, land plaster.
Shorten the method in its cultivation cycle when 3, Asparagus is produced, the quality requirement of flammulina velutipes liquid strains in the technical scheme: the liquid spawn cultivation period at 10 days with even, energetic, good, the free of contamination liquid spawn of quality of interior, mycelia (ball) size.
Shorten the method in its cultivation cycle when 4, Asparagus is produced, the cultivating container that is used for fruiting in the technical scheme is selected: but the wide-mouth plastic bottle that white is transparent, capacity is the 850ml high temperature high voltage resistant.
Shorten the method in its cultivation cycle when 5, Asparagus is produced, the preparation of mushroom producing culture matrix and management of producing mushroom method are following in the technical scheme:
(1) culture base-material bottling: with the culture base-material culture bottle of packing into, every bottledly to shoulder, seal;
(2) mattress that goes out: the blake bottle that culture matrix will be housed is placed into inherent 1.4～1.5 kilograms/cm of pressure cooker
2Pressure, temperature are to keep sterilization 2 hours under 115～121 ℃ the high pressure, hot conditions; When the inherent temperature of normal pressure pot has reached 100 ℃ and keep such temperature sterilization 6～8 hours;
(3) loose metal: after the sterilization, while hot blake bottle is trembled, buckled, shake up culture matrix in the bottle, in case culture matrix hardens into figure in the bottle;
(4) inoculation: under gnotobasis, insert flammulina velutipes liquid strains, inoculum concentration is 25～30ml/ bottle;
(5) material shaking: after inserting liquid spawn, again blake bottle is trembled, buckled, make the interior culture matrix comprehensive engagement of liquid spawn and bottle;
(6) cultivation: postvaccinal blake bottle is moved to the culturing room lucifuge cultivate, and be 60～65% at 22～25 ℃, relative air humidity, cover with bottle through cultivating 10～12 days mycelia with the temperature adjusting of culturing room;
(7) management of producing mushroom: the blake bottle that will cover with mycelia moves on to the mushroom producing culture chamber, and gathers and even the completion of whole Asparagus recovery process by Routine Management to fruit body.
The existing following advantage of the present invention:
1, proposed by the invention enrich production technology simple, be prone to implement.
2, the used primary raw material of embodiment of the present invention---paddy, its raw material sources are extensive, easily tissue and cheap; In addition, paddy kernel is not only nutritious, and the culture base-material of its formation also can not harden, and helps inoculating the back bacterial classification and is prone to disperse and be penetrated in the culture base-material.
3, the present invention is in implementation process, and the water content of culture base-material is controlled at about 50%, and such behave is for when the inoculation, can the multiple access liquid spawn, and the bacterial classification amount is big, and it is many to sprout point, can cover with bottle soon; And at ordinary times; When the water content of raw material in the bottle 55～60% the time; Insert the liquid spawn amount and can only be controlled at below the 10ml/ bottle, as insert the water content that too much liquid spawn will increase bottle interior raw material, the culture base-material environment that humidity is overweight can influence sprouting and the growth of mycelia on the contrary.
4, the present invention has used liquid spawn and has inoculated; Liquid spawn is cultivated and can obtain in 3～5 days; Simultaneously liquid spawn not only inoculates simple and efficiently, but also has flowable, characteristics such as is prone to disperse, may penetrate in the material, and the inoculation back sends out that bacterium point is many, the bacterial classification sprouting is quick.
5, the present invention is in implementation process, and the blake bottle after the sterilization through trembling, buckle loose metal, is prevented that effectively bottle interior the culture base-material from hardening, after inserting liquid spawn, liquid spawn in loose culture base-material, receive when mobile resistance few, permeate darker; And insert blake bottle behind the liquid spawn once more through trembling bottle, liquid spawn is spread all in the bottle very soon, and can with the culture base-material comprehensive engagement, bacterial classification is once sprout, cover with soon bottle.
6, through embodiment of the present invention, preparing its whole cycle of mushroom culture medium matter only needs 25～27 days, and traditional method needs nearly 3 months, and production efficiency improves greatly; Comparable conventional method mode is cultivated more than two batches more in 1 year, and the availability in place increases substantially.
7, through embodiment of the present invention, more help Asparagus batch production, intensification, large-scale production.
Embodiment
Below in conjunction with embodiment method of the present invention is further specified.
Asparagus shortens the method in its cultivation cycle when producing, embodiment is following:
Shorten the method in its cultivation cycle when 1, Asparagus is produced; Comprise in the technical scheme: using with grain is that primary raw material prepares culture base-material; The quality requirement of flammulina velutipes liquid strains, the cultivating container that is used for fruiting is selected the preparation of mushroom producing culture matrix and management of producing mushroom method.
Shorten the method in its cultivation cycle when 2, Asparagus is produced, use with grain in the technical scheme that to be that primary raw material prepares the method for culture base-material following:
(1) raw material and the proportioning formed: paddy 82%, weed tree sawdust 9%, wheat bran 5%, corn flour 3%, land plaster 1%, water is an amount of;
(2) preparation: earlier place clear water to soak paddy and pull the elimination excessive moisture out after 40～48 hours; Then mix and the resulting mixture that stirs, admix an amount of clear water at last and about 50%, form culture base-material until the water content of mixture with weed tree sawdust, wheat bran, corn flour, land plaster.
Shorten the method in its cultivation cycle when 3, Asparagus is produced, the quality requirement of flammulina velutipes liquid strains in the technical scheme: the liquid spawn cultivation period at 10 days with even, energetic, good, the free of contamination liquid spawn of quality of interior, mycelia (ball) size.
Shorten the method in its cultivation cycle when 4, Asparagus is produced, the cultivating container that is used for fruiting in the technical scheme is selected: but the wide-mouth plastic bottle that white is transparent, capacity is the 850ml high temperature high voltage resistant.
Shorten the method in its cultivation cycle when 5, Asparagus is produced, the preparation of mushroom producing culture matrix and management of producing mushroom method are following in the technical scheme:
(1) culture base-material bottling: with the culture base-material culture bottle of packing into, every bottledly to shoulder, seal;
(2) mattress that goes out: the blake bottle that culture matrix will be housed is placed into inherent 1.4～1.5 kilograms/cm of pressure cooker
2Pressure, temperature are to keep sterilization 2 hours under 115～121 ℃ the high pressure, hot conditions; When the inherent temperature of normal pressure pot has reached 100 ℃ and keep such temperature sterilization 6～8 hours;
(3) loose metal: after the sterilization, while hot blake bottle is trembled, buckled, shake up culture matrix in the bottle, in case culture matrix hardens into figure in the bottle;
(4) inoculation: under gnotobasis, insert flammulina velutipes liquid strains, inoculum concentration is 25～30ml/ bottle;
(5) material shaking: after inserting liquid spawn, again blake bottle is trembled, buckled, make the interior culture matrix comprehensive engagement of liquid spawn and bottle;
(6) cultivation: postvaccinal blake bottle is moved to the culturing room lucifuge cultivate, and be 60～65% at 22～25 ℃, relative air humidity, cover with bottle through cultivating 10～12 days mycelia with the temperature adjusting of culturing room;
(7) management of producing mushroom: the blake bottle that will cover with mycelia moves on to the mushroom producing culture chamber, and gathers and even the completion of whole Asparagus recovery process by Routine Management to fruit body.
Claims (5)
1. shorten the method in its cultivation cycle when Asparagus is produced; Its characteristic comprises: using with grain is that primary raw material prepares culture base-material; The quality requirement of flammulina velutipes liquid strains, the cultivating container that is used for fruiting is selected the preparation of mushroom producing culture matrix and management of producing mushroom method.
2. shorten the method in its cultivation cycle when producing according to the Asparagus described in the claim 1, it is characterized in that using with grain that to be that primary raw material prepares the method for culture base-material following:
(1) raw material and the proportioning formed: paddy 82%, weed tree sawdust 9%, wheat bran 5%, corn flour 3%, land plaster 1%, water is an amount of;
(2) preparation: earlier place clear water to soak paddy and pull the elimination excessive moisture out after 40～48 hours; Then mix and the resulting mixture that stirs, admix an amount of clear water at last and about 50%, form culture base-material until the water content of mixture with weed tree sawdust, wheat bran, corn flour, land plaster.
3. shorten the method in its cultivation cycle when producing according to the Asparagus described in the claim 1, it is characterized in that the quality requirement of flammulina velutipes liquid strains: the liquid spawn cultivation period at 10 days with even, energetic, good, the free of contamination liquid spawn of quality of interior, mycelia (ball) size.
4. shorten the method in its cultivation cycle when producing according to the Asparagus described in the claim 1, it is characterized in that being used for the cultivating container selection of fruiting: but the wide-mouth plastic bottle that white is transparent, capacity is the 850ml high temperature high voltage resistant.
5. shorten the method in its cultivation cycle when producing according to the Asparagus described in the claim 1, it is characterized in that the preparation of mushroom producing culture matrix and management of producing mushroom method are following:
(1) culture base-material bottling: with the culture base-material culture bottle of packing into, every bottledly to shoulder, seal;
(2) mattress that goes out: the blake bottle that culture matrix will be housed is placed into inherent 1.4～1.5 kilograms/cm of pressure cooker
2Pressure, temperature are to keep sterilization 2 hours under 115～121 ℃ the high pressure, hot conditions; When the inherent temperature of normal pressure pot has reached 100 ℃ and keep such temperature sterilization 6～8 hours;
(3) loose metal: after the sterilization, while hot blake bottle is trembled, buckled, shake up culture matrix in the bottle, in case culture matrix hardens into figure in the bottle;
(4) inoculation: under gnotobasis, insert flammulina velutipes liquid strains, inoculum concentration is 25～30ml/ bottle;
(5) material shaking: after inserting liquid spawn, again blake bottle is trembled, buckled, make the interior culture matrix comprehensive engagement of liquid spawn and bottle;
(6) cultivation: postvaccinal blake bottle is moved to the culturing room lucifuge cultivate, and be 60～65% at 22～25 ℃, relative air humidity, cover with bottle through cultivating 10～12 days mycelia with the temperature adjusting of culturing room;
(7) management of producing mushroom: the blake bottle that will cover with mycelia moves on to the mushroom producing culture chamber, and gathers and even the completion of whole Asparagus recovery process by Routine Management to fruit body.
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| CN2012101122878A CN102630483A (en) | 2012-04-16 | 2012-04-16 | Method for shortening cultivation period of needle mushroom during production |
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Cited By (8)
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| CN103342614A (en) * | 2013-07-18 | 2013-10-09 | 邬金飞 | Pleurotus nebrodensis protospecies compost formula and manufacturing method of compost |
| CN104119126A (en) * | 2013-04-26 | 2014-10-29 | 如意情集团股份有限公司 | Production process for flammulina velutipes medium |
| CN104303837A (en) * | 2014-10-13 | 2015-01-28 | 上海市农业科学院 | Secondary fungus culture method for mushroom factory production |
| CN105052534A (en) * | 2015-07-13 | 2015-11-18 | 河北大学 | Method for cultivating needle mushrooms by using wild jujube branch crumbs |
| CN105087389A (en) * | 2015-07-18 | 2015-11-25 | 周永和 | Method for producing black fungus primary strain by virtue of solid mixed medium |
| CN105112300A (en) * | 2015-08-31 | 2015-12-02 | 雷色香 | Needle mushroom mycelium blocks |
| CN105123266A (en) * | 2015-08-31 | 2015-12-09 | 雷色香 | Enoki mushroom powder |
| CN111955286A (en) * | 2020-08-28 | 2020-11-20 | 平泉市希才应用菌科技发展有限公司 | Preparation method of edible fungus liquid strain |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104119126A (en) * | 2013-04-26 | 2014-10-29 | 如意情集团股份有限公司 | Production process for flammulina velutipes medium |
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| CN104303837A (en) * | 2014-10-13 | 2015-01-28 | 上海市农业科学院 | Secondary fungus culture method for mushroom factory production |
| CN105052534A (en) * | 2015-07-13 | 2015-11-18 | 河北大学 | Method for cultivating needle mushrooms by using wild jujube branch crumbs |
| CN105087389A (en) * | 2015-07-18 | 2015-11-25 | 周永和 | Method for producing black fungus primary strain by virtue of solid mixed medium |
| CN105112300A (en) * | 2015-08-31 | 2015-12-02 | 雷色香 | Needle mushroom mycelium blocks |
| CN105123266A (en) * | 2015-08-31 | 2015-12-09 | 雷色香 | Enoki mushroom powder |
| CN111955286A (en) * | 2020-08-28 | 2020-11-20 | 平泉市希才应用菌科技发展有限公司 | Preparation method of edible fungus liquid strain |
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Application publication date: 20120815 |