CN112806215A - Method for preparing mushroom liquid strain and method for producing mushroom stick - Google Patents
Method for preparing mushroom liquid strain and method for producing mushroom stick Download PDFInfo
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- CN112806215A CN112806215A CN202110305539.8A CN202110305539A CN112806215A CN 112806215 A CN112806215 A CN 112806215A CN 202110305539 A CN202110305539 A CN 202110305539A CN 112806215 A CN112806215 A CN 112806215A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/30—Accessories for use before inoculation of spawn, e.g. sterilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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Abstract
The invention relates to a method for preparing mushroom liquid strains and a method for matching and producing mushroom sticks, which comprises the following steps: (1) preparing a secondary liquid strain culture medium; (2) sterilizing and inoculating the second-level liquid strain; (3) culturing a secondary liquid strain; (4) preparing a solid high-quality fungus stick cultivation material of the third-class strain; (5) sterilizing and inoculating the third-class solid high-quality fungus sticks; (6) and (5) culturing the third-class solid high-quality fungus sticks. The invention adopts a brand new strain formula for culturing the second-stage liquid strains of the mushrooms, has the advantages of short culture time, high purity, strong activity and the like, has high survival rate after inoculation, is quick to spawn, can shorten the culture time of the third-stage solid high-quality fungus sticks, reduces the labor cost, and is beneficial to improving the yield and the quality of the mushrooms.
Description
Technical Field
The invention relates to the field of edible mushroom cultivation, in particular to a method for manufacturing mushroom liquid strains and matching production of high-quality environment-friendly mushroom sticks.
Background
Shiitake mushroom (Lentinusedodes) Belonging to the order Agaricales (Agaricales) Tricholomataceae (A. B)Tricholomatacete) Lentinus (genus: (A)Lentinus) The edible fungus is the second largest edible fungus in the world, one of the largest edible fungi in China, and is a famous edible and medicinal fungus. The mushroom is rich in nutrition, delicious in taste and pleasant in flavor, and the amino acid content necessary for a human body is rich and accounts for about 35.7 percent of the total amount of the amino acid. At present, the technology for preparing the liquid mushroom spawn is mature day by day, the liquid mushroom spawn has short production period and regular fungus age, and the solid mushroom spawn is inoculated by the liquid mushroom spawn, so that the liquid mushroom spawn has the advantages of quick flowing, easy dispersion, quick germination, quick field planting and the like.
Disclosure of Invention
The invention aims to screen and optimize carbon sources, nitrogen sources, inorganic salts and the like of second-level species and third-level species bacterium sticks, provides a liquid strain formula and a high-quality bacterium stick formula for a mushroom planting technology, aims to plant high-yield and high-quality mushroom bacterium sticks, improves the bacterium growing efficiency and shortens the bacterium growing time.
The technical scheme of the invention is as follows: a method for preparing mushroom liquid strains comprises the following steps:
(1) preparing a second-grade mushroom liquid strain culture medium: the components by mass percentage are as follows: 10% of corn flour, 6% of brown sugar, 2% of glucose, 1% of bran, 0.5% of monopotassium phosphate, 0.2% of magnesium sulfate heptahydrate, 0.1% of edible oil and VB1 10mg, the balance being distilled water, to constant volume1000mL of the solution;
(2) and (3) secondary liquid strain sterilization and inoculation: sterilizing at 121 ℃ for more than 60 min, cooling to 23-28 ℃, and inoculating the mother strains of the mushrooms;
(3) and (3) secondary liquid strain culture: inoculating Lentinus Edodes strain, dark culturing at 25 + -2 deg.C in 110r/min constant temperature shaking table, and obtaining second-stage liquid strain with uniform cell size and clear culture medium within 5 d.
The pH value of the secondary liquid strain culture medium in the step (1) is 6.0-6.5.
And (3) sterilizing at the high temperature of 121 ℃ for 60 min in the step (2), cooling to 25 ℃, inoculating the mushroom stock, wherein the stock is the dominant hypha obtained by inoculating the preserved mushroom slant culture medium to the activated plate culture medium.
The invention relates to a matching production method of high-quality solid fungus sticks, which comprises the following steps:
(1) preparing a solid fungus stick cultivation material for the third-class strain: weighing the cultivation raw materials according to the mass percentage, and preparing a formula of the three-stage solid fungus stick cultivation material: 71% of broad-leaved tree sawdust, 8% of corncob scraps, 18% of bran, 1% of gypsum, 0.5% of lime, 0.5% of calcium carbonate, 0.5% of corn flour and 0.5% of soybean meal;
(2) sterilizing and inoculating a three-stage strain solid bacteria stick: sterilizing the fungus sticks obtained in the step (1) at the high temperature and high pressure of 121 ℃ and in the environment of 101kpa for more than 4 hours; cooling the fungus bags to 25-28 ℃; respectively injecting 5 mL of the secondary liquid strain obtained in the step (3) to 1/5, 2/5, 3/5 and 4/5 positions of a bacteria stick by using a 5 mL sterile injector on an ultra-clean workbench, and directly puncturing the surface of the bacteria stick; promoting the bacterial liquid to be uniformly distributed on the surface of the material to finish inoculation;
(3) culturing a third-class solid fungus stick: and (3) placing the fungus sticks inoculated in the step (2) on a culture frame, transferring the fungus sticks to a culture room for dark ventilation culture, and obtaining the fungus sticks which can be moved to a greenhouse shelf for culture after hyphae fully grow.
The raw materials of the three-level strain cultivation material are weighed according to the mass percentage in the step (1), are uniformly stirred to prepare the cultivation material with the matrix water content of 55% -60% and the pH value of 6.0, then are filled into a polyethylene fungus bag with the specification of 17cm multiplied by 55cm multiplied by 0.005cm, a sheathing bag is sheathed after the sealing, the opening of the sheathing bag is sealed by a lantern ring combination, and the length of a fungus stick is 44cm +/-1 cm.
In the step (2): sterilizing the fungus sticks obtained in the step (4) at high temperature and high pressure for more than 4 hours at the temperature of 121 ℃ and under the environment of 101 kpa; cooling the fungus bag to 25 ℃; respectively injecting 5 mL of the secondary liquid strain obtained in the step (3) to 1/5, 2/5, 3/5 and 4/5 positions of a bacteria stick by using a 5 mL sterile injector on an ultra-clean workbench, and directly puncturing the surface of the bacteria stick; promoting the bacterial liquid to be uniformly distributed on the surface of the material to finish inoculation;
and (3) placing the inoculated bacteria sticks obtained in the step (5) on a culture frame of a culture room, culturing the bacteria at a constant temperature of about 25 ℃, and keeping the humidity at 40-60% for dark ventilation culture.
Compared with the prior art, the invention has the advantages that: by adopting the technical operation, the second-stage liquid spawn of the mushroom is short in culture time, high in purity, fast and neat in spawn running, strong in hypha and more beneficial to inoculation of third-stage spawn, the hypha full-bag time of the inoculated mushroom sticks is shortened compared with the hypha sticks inoculated by solid spawn, meanwhile, the cultivation material of the third-stage spawn sticks is made of raw materials such as environment-friendly carbon sources and nitrogen sources, the cost is saved, and the quality of the planted mushroom is better.
Detailed Description
A method for preparing mushroom liquid strains and a matching production method of high-quality environment-friendly mushroom sticks comprises the following steps:
(1) preparing a second-grade mushroom liquid strain culture medium: the components by mass percentage are as follows: 10% of corn flour, 6% of brown sugar, 2% of glucose, 1% of bran, 0.5% of monopotassium phosphate, 0.2% of magnesium sulfate heptahydrate, 0.1% of edible oil and VB1 10mg, and the balance of distilled water, and the volume is fixed to 1000 mL;
(2) and (3) secondary liquid strain sterilization and inoculation: sterilizing at 121 deg.C for more than 60 min, cooling to 25 deg.C, inoculating Lentinus Edodes;
(3) and (3) secondary liquid strain culture: inoculating Lentinus Edodes strain, dark culturing in constant temperature shaking table at 25 deg.C and 110r/min, and obtaining second-stage liquid strain with uniform cell size and clear culture medium within 5 d; the culture time of the liquid strains is only 5 days, the solid strains can grow over the strain bags for at least two months, the liquid strains have low culture pollution rate and high purity, the strains grow well, the hyphae are strong, the inoculation of the third-level strains is more convenient, the second-level liquid strains are directly injected into the third-level strains in the inoculation process, and the pollution rate is low; the second-stage solid strain also needs to break the strain rod into small fragments and then put into the third-stage strain, so the operation process is complex and long in time, and the pollution rate is high.
(4) Preparing a solid fungus stick cultivation material for the third-class strain: weighing the cultivation raw materials according to the mass percentage, and preparing a formula of the three-stage solid fungus stick cultivation material: 71% of broad-leaved tree sawdust, 8% of corncob scraps, 18% of bran, 1% of gypsum, 0.5% of lime, 0.5% of calcium carbonate, 0.5% of corn flour and 0.5% of soybean meal; uniformly stirring the mixture to prepare a cultivation material with the matrix of 55-60 percent of water content and the pH value of 6.0, then filling the cultivation material into a polyethylene fungus bag with the specification of 17cm multiplied by 55cm multiplied by 0.005cm, sealing the bag opening, sleeving a bag, sealing the bag opening by using a lantern ring combination, and ensuring the length of a fungus stick to be 44cm +/-1 cm.
(5) Sterilizing and inoculating a three-stage strain solid bacteria stick: sterilizing the fungus sticks obtained in the step (4) at the high temperature and high pressure of 121 ℃ and in the environment of 101kpa for more than 4 hours; cooling the fungus bags to 25-28 ℃; respectively injecting 5 mL of the secondary liquid strain obtained in the step (3) to 1/5, 2/5, 3/5 and 4/5 positions of a bacteria stick by using a 5 mL sterile injector on an ultra-clean workbench, and directly puncturing the surface of the bacteria stick; promoting the bacterial liquid to be uniformly distributed on the surface of the material to finish inoculation;
(6) culturing a third-class solid fungus stick: and (4) placing the fungus sticks inoculated in the step (5) on a culture frame, transferring the fungus sticks to a culture room for dark ventilation culture, and obtaining the fungus sticks which can be moved to a greenhouse shelf for culture after hyphae fully grow.
The technical features of the present invention are described above, and the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A method for preparing mushroom liquid strains is characterized by comprising the following steps:
(1) preparing a second-grade mushroom liquid strain culture medium: componentsThe weight percentages are as follows: 10% of corn flour, 6% of brown sugar, 2% of glucose, 1% of bran, 0.5% of monopotassium phosphate, 0.2% of magnesium sulfate heptahydrate, 0.1% of edible oil and VB1 10mg, and the balance of distilled water, and the volume is fixed to 1000 mL;
(2) and (3) secondary liquid strain sterilization and inoculation: sterilizing at 121 deg.C for more than 60 min, cooling to 23-28 deg.C, inoculating Lentinus Edodes stock;
(3) and (3) secondary liquid strain culture: inoculating Lentinus Edodes strain, dark culturing at 25 + -2 deg.C in 110r/min constant temperature shaking table, and obtaining second-stage liquid strain with uniform cell size and clear culture medium within 5 d.
2. The method for producing a liquid spawn of Lentinus edodes according to claim 1, wherein the secondary liquid spawn culture medium in step (1) has a pH of 6.0 to 6.5.
3. The method for preparing liquid spawn of Lentinus edodes according to claim 1, wherein in step (2), the liquid spawn of Lentinus edodes is sterilized at 121 deg.C for 60 min, cooled to 25 deg.C, and inoculated with Lentinus edodes stock culture, which is the activated dominant hypha of Lentinus edodes slant culture medium.
4. A matching production method of high-quality solid fungus sticks is characterized by comprising the following steps:
(1) preparing a solid fungus stick cultivation material for the third-class strain: weighing the cultivation raw materials according to the mass percentage, and preparing a formula of the three-stage solid fungus stick cultivation material: 71% of broad-leaved tree sawdust, 8% of corncob scraps, 18% of bran, 1% of gypsum, 0.5% of lime, 0.5% of calcium carbonate, 0.5% of corn flour and 0.5% of soybean meal;
(2) sterilizing and inoculating a three-stage strain solid bacteria stick: sterilizing the fungus sticks obtained in the step (1) at the high temperature and high pressure of 121 ℃ and in the environment of 101kpa for more than 4 hours; cooling the fungus bags to 25-28 ℃; injecting 5 mL of the secondary liquid strain prepared by the method in claim 1 into 1/5, 2/5, 3/5 and 4/5 positions of a bacteria stick respectively by using a 5 mL sterile syringe on an ultra-clean bench, and directly puncturing the surface of the bacteria stick; promoting the bacterial liquid to be uniformly distributed on the surface of the cultivation material to finish inoculation;
(3) culturing a third-class solid fungus stick: and (3) placing the fungus sticks inoculated in the step (2) on a culture frame, transferring the fungus sticks to a culture room for dark ventilation culture, and obtaining the fungus sticks which can be moved to a greenhouse shelf for culture after hyphae fully grow.
5. The method for producing high-quality solid mushroom sticks as claimed in claim 4, wherein the step (1) comprises weighing the raw materials of the third-class mushroom culture material according to the mass percentage, uniformly stirring the raw materials to prepare the culture material with the substrate water content of 55-60% and the pH value of 6.0, putting the culture material into a polyethylene mushroom bag with the specification of 17cm x 55cm x 0.005cm, sealing the bag, sleeving the bag, sealing the bag opening with a lantern ring combination, and the length of the mushroom stick is 44cm +/-1 cm.
6. The method for producing high-quality solid mushroom sticks according to claim 4, wherein in the step (2): the fungus sack was cooled to 25 ℃.
7. The method for producing the high-quality solid fungus sticks in a matched mode according to claim 4, wherein the fungus sticks inoculated in the step (2) are placed on a culture frame of a culture room, fungus is cultured at a constant temperature of 25 ℃, and humidity is kept between 40% and 60% in a dark ventilation mode for culture.
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| CN202110305539.8A CN112806215A (en) | 2021-03-23 | 2021-03-23 | Method for preparing mushroom liquid strain and method for producing mushroom stick |
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| CN202110305539.8A CN112806215A (en) | 2021-03-23 | 2021-03-23 | Method for preparing mushroom liquid strain and method for producing mushroom stick |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113892393A (en) * | 2021-10-17 | 2022-01-07 | 夏建红 | Shiitake mushroom stick for plateau area and preparation method thereof |
Citations (5)
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| CN104956918A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Method for preparing mushroom sticks |
| CN105176839A (en) * | 2015-09-10 | 2015-12-23 | 井冈山井祥菌草生态科技股份有限公司 | Culture medium of mushroom liquid strain and method of producing mushroom bacterium stick |
| CN108967038A (en) * | 2018-08-28 | 2018-12-11 | 铜陵盛牛菌业有限责任公司 | A kind of method of liquid strain cultivation mushroom |
| CN108990693A (en) * | 2018-07-05 | 2018-12-14 | 贵州润瑞菌业有限责任公司 | A kind of mushroom crossbreeding and breeding method |
| CN109197382A (en) * | 2018-09-06 | 2019-01-15 | 凤台诺恒农业发展有限公司 | A kind of method that batch production prepares lentinus edodes strain stick |
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2021
- 2021-03-23 CN CN202110305539.8A patent/CN112806215A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104956918A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Method for preparing mushroom sticks |
| CN105176839A (en) * | 2015-09-10 | 2015-12-23 | 井冈山井祥菌草生态科技股份有限公司 | Culture medium of mushroom liquid strain and method of producing mushroom bacterium stick |
| CN108990693A (en) * | 2018-07-05 | 2018-12-14 | 贵州润瑞菌业有限责任公司 | A kind of mushroom crossbreeding and breeding method |
| CN108967038A (en) * | 2018-08-28 | 2018-12-11 | 铜陵盛牛菌业有限责任公司 | A kind of method of liquid strain cultivation mushroom |
| CN109197382A (en) * | 2018-09-06 | 2019-01-15 | 凤台诺恒农业发展有限公司 | A kind of method that batch production prepares lentinus edodes strain stick |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113892393A (en) * | 2021-10-17 | 2022-01-07 | 夏建红 | Shiitake mushroom stick for plateau area and preparation method thereof |
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