CN106831037A - A kind of edible fungus liquid culture growth medium and preparation method thereof - Google Patents
A kind of edible fungus liquid culture growth medium and preparation method thereof Download PDFInfo
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- CN106831037A CN106831037A CN201510873397.XA CN201510873397A CN106831037A CN 106831037 A CN106831037 A CN 106831037A CN 201510873397 A CN201510873397 A CN 201510873397A CN 106831037 A CN106831037 A CN 106831037A
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Abstract
The invention provides a kind of edible fungus liquid culture growth medium, including the aqueous solution, the aqueous solution includes the raw material of following weight ratio ingredient：Dusty yeast 0.1-0.5%, sugar 1.5-5%, bean powder 0.1-0.5%, potassium dihydrogen phosphate 0.05-0.1%, magnesium sulfate 0.05-0.1%.The preparation method of the culture medium mainly comprises the following steps：1）Culture medium is configured：By the sucrose in raw material, magnesium sulfate, potassium dihydrogen phosphate is soluble in water is sufficiently stirred for, until being dissolved into semi-finished product solution；Again by stirring and dissolving in the bean powder in the raw material, corn flour, the dusty yeast addition semi-finished product solution, medium liquid is brewed to；2）Culture medium is adjusted：The pH value of the medium liquid is adjusted with PH conditioning agents；3）Medium sterilization；4）Culture medium inoculated.The present invention can improve the survival rate of hypha of edible fungus, hypha of edible fungus is grown fast, energetic, good stability.
Description
Technical field
The present invention relates to bioengineering field, more particularly to a kind of culture medium and preparation method thereof.
Background technology
Strain used by Edible Fungi has solid spawn and liquid spawn; liquid spawn has the advantages such as incubation time is short, hair bacterium is fast, biological transformation ratio is high, strain purity is high with respect to solid spawn; and scale of being more convenient for, continuous production, it is the trend of mushroom industry batch production method cane technological innovation.Existing edible fungus liquid culture growth medium exists and is prepared into that power is low, mycelial growth is not suitable with present, after incubation the phase easily there is the problem of the aging regression of mycelia, and operation professional technique requirement is high, the shortcomings of do not possess versatility, it is impossible to high-volume popularization is used.
The content of the invention
It is an object of the present invention to provide a kind of edible fungus liquid culture growth medium, solves above technical problem.
Another object of the present invention is to, there is provided a kind of preparation method of edible fungi liquid strain, solve above technical problem.
Technical problem solved by the invention can be realized using following technical scheme：
Edible fungus liquid culture growth medium, including raw material, the aqueous solution, the aqueous solution are contained within the raw material, it is characterised in that in the aqueous solution, raw material include following weight than component：Dusty yeast 0.1-0.5%, sugar 1.5-5%, bean powder 0.1-0.5%, potassium dihydrogen phosphate 0.05-0.1%, magnesium sulfate 0.05-0.1%.
The raw material also includes corn flour 0.3-0.5%.
The sugar can use sucrose, glucose or maltose can also be used, it is preferred that using sucrose, with respect to other sugars, sucrose is more suitable for the carbon source of edible mushroom, and the reaction between the sucrose and dusty yeast, the reaction between versus glucose, maltose and dusty yeast is more stable, cost is more cheap, and utilizing status are more preferably.
The raw material preferably includes following weight than combination：Dusty yeast 0.2%, sucrose 3%, bean powder 0.2%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.08%, corn flour 0.2%.
Said components are most basic component in the present invention, and these components mostly abundance, cheap, are easy to largely use, and carry out volume production.And the hypha of edible fungus cultivated using the present invention, quickly, energetic, mycelial density is higher in same sprout time for mycelial growth.
The raw material also includes following density fractions：Vitamin 0-0.5%, folic acid 0-0.5%.
Using at least one in vitamin B1 or vitamin B2, vitamin B1, vitamin B2 can promote the growth of hypha of edible fungus to the vitamin.
Folic acid can promote the growth of hypha of edible fungus as the nitrogen source needed for a kind of growth of hypha of edible fungus, appropriate folic acid.
The preparation method of edible fungus liquid culture growth medium, it is characterised in that comprise the following steps：
1）Culture medium is configured：By the sucrose in the raw material, magnesium sulfate, potassium dihydrogen phosphate is soluble in water is sufficiently stirred for, until being dissolved into semi-finished product solution；Again by stirring and dissolving in the bean powder in the raw material, corn flour, the dusty yeast addition semi-finished product solution, medium liquid is brewed to.
2）Culture medium is adjusted：The pH value of the medium liquid is adjusted with PH conditioning agents.
3）Medium sterilization：The medium liquid is poured into fermentation tank, the sterilizing of at least 30 minutes is carried out in feeding sterilizing installation, in the case where nutrition is not lost, kill the bacterium in the medium liquid.
4）Inoculation：After the completion of sterilizing, medium liquid is cooled to 25 DEG C, accesses edible fungus species, and adjust to 20-22 DEG C the liquid medium temperature, then, the medium liquid is carried out into aerobic culture, fermented and cultured can have to edible fungus species best in quality in 7-10 days.
Step 3）In, when being sterilized to the medium liquid, sterilising temp is 121-125 DEG C, and pressure is 0.105-0.135Mpa.Under high-temperature high-pressure state, the bacterium in the medium liquid can be preferably removed, it is to avoid hypha of edible fungus is contaminated in cultivating process.
Beneficial effect：Due to using above-mentioned technical proposal, the present invention to improve the survival rate of hypha of edible fungus, hypha of edible fungus is set to grow fast, energetic, good stability, after inoculation plantation, mycelia strong stress resistance is difficult aging, can fast-growth sprouting.
Specific embodiment
Edible fungus liquid culture growth medium, including raw material, the aqueous solution, the aqueous solution are contained within raw material, in aqueous, raw material include following weight than component：Dusty yeast 0.1-0.5%, sugar 1.5-5%, bean powder 0.1-0.5%, potassium dihydrogen phosphate 0.05-0.1%, magnesium sulfate 0.05-0.1%.Raw material also includes corn flour 0.3-0.5%.Sugar can use sucrose, glucose or maltose can also be used, it is preferred that using sucrose, with respect to other sugars, sucrose is more suitable for the carbon source of edible mushroom, and the reaction between sucrose and dusty yeast, the reaction between versus glucose, maltose and dusty yeast is more stable, cost is more cheap, and utilizing status are more preferably.
Raw material preferably includes following weight than combination：Dusty yeast 0.2%, sucrose 3%, bean powder 0.2%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.08%, corn flour 0.2%.Said components are most basic component in the present invention, and these components mostly abundance, cheap, are easy to largely use, and carry out volume production.And the hypha of edible fungus cultivated using the present invention, quickly, energetic, mycelial density is higher in same sprout time for mycelial growth.
Raw material also includes following density fractions：Vitamin 0-0.5%, folic acid 0-0.5%.Using at least one in vitamin B1 or vitamin B2, vitamin B1, vitamin B2 can promote the growth of hypha of edible fungus to vitamin.And folic acid grows required nitrogen source as a kind of hypha of edible fungus, appropriate folic acid can promote the growth of hypha of edible fungus.
Bean powder can provide enough nitrogen nutriment, be conducive to mycelia fast-growth as quick-acting nitrogen sources as effect nitrogen source late, yeast powder for the growth of hypha of edible fungus.And sucrose, corn flour can be engaged promotion mycelial growth as carbon source with nitrogen source, and the weight between carbon source and nitrogen source is than controlling 5:1 to 20:1, preferably 10:1.
In practical operation, can fall into vitamin and folic acid and suitably adjusted on the basis of raw material preferred compositions.
Embodiment 1：
Raw material includes following weight than combination：Dusty yeast 0.3%, sucrose 2%, bean powder 0.2%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.08%, corn flour 0.2%, vitamin 0.1%.
It is sufficiently stirred for the sucrose in raw material, magnesium sulfate, potassium dihydrogen phosphate, vitamin are soluble in water according to the above ratio, until being dissolved into semi-finished product solution；Bean powder again in raw material, corn flour, dusty yeast are proportionally added into stirring and dissolving in semi-finished product solution, are brewed to medium liquid.
PH is being carried out to medium liquid with hydrochloric acid to adjusting, its pH value is controlled in 6.5-7.0, keeping neutral, so as to edible fungi growth.Then the medium liquid for finishing will be modulated to be put into fermentation tank, and fermentation tank is put into sterilizing installation and sterilized at least 30 minutes, during sterilizing, 121-123 DEG C of keeping temperature, pressure is 0.11-0.12Mpa.Under high-temperature high-pressure state, the bacterium in medium liquid can be preferably removed, it is to avoid hypha of edible fungus is contaminated in cultivating process.
Medium liquid is then cooled to 25 DEG C, and prepare to access edible bacterial vaccine inoculation in liquid medium body, during inoculation, the temperature control of culture medium solution is at 20-21 DEG C, after the completion of inoculation, culture medium containing edible fungus species is put into aerobic raising chamber, the aerobic fermentation culture of 7-10 days by a definite date is carried out.
Embodiment 2：
Raw material includes following weight than combination：Dusty yeast 0.35%, glucose 3%, bean powder 0.2%, potassium dihydrogen phosphate 0.06%, magnesium sulfate 0.06%, corn flour 0.3%, vitamin 0.1%, folic acid 0.1%.
Raw material is mixed according to the above ratio and is poured into the aqueous solution, stirring and dissolving after being brewed to medium liquid, is adjusted using potassium dihydrogen phosphate, dipotassium hydrogen phosphate to the pH value of medium liquid, the pH value of medium liquid is tended to neutral.Then fermentation tank is being poured into medium liquid, is being put into sterilizing installation the high-temperature sterilization for carrying out 1 hour, 123-125 DEG C of sterilising temp is being kept during sterilizing, pressure is being 0.120-0.135Mpa.Medium liquid is then cooled to 25 DEG C, begin preparing for accessing edible fungus species, and after liquid medium temperature is adjusted to 21-22 DEG C is inoculated with, medium liquid containing edible fungus species is put into fermentation tank, send into aerobic raising chamber, fermentation is cultivated 7-10 days and can obtain edible fungus species best in quality.
Claims (8)
1. a kind of edible fungus liquid culture growth medium, it is characterised in that including the aqueous solution, the aqueous solution includes the raw material of following weight ratio ingredient：Dusty yeast 0.1-0.5%, sugar 1.5-5%, bean powder 0.1-0.5%, potassium dihydrogen phosphate 0.05-0.1%, magnesium sulfate 0.05-0.1%.
2. edible fungus liquid culture growth medium according to claim 1, it is characterised in that：The raw material also includes corn flour 0.3-0.5%.
3. edible fungus liquid culture growth medium according to claim 1, it is characterised in that：The sugar uses sucrose.
4. edible fungus liquid culture growth medium according to claim 3, it is characterised in that：The raw material includes following weight than combination：Dusty yeast 0.2%, sucrose 3%, bean powder 0.2%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.08%, corn flour 0.2%.
5. edible fungus liquid culture growth medium according to claim 4, it is characterised in that：The raw material also includes following density fractions：Vitamin 0-0.5%, folic acid 0-0.5%.
6. edible fungus liquid culture growth medium according to claim 4, it is characterised in that：The vitamin is using at least one in vitamin B 1 or vitamin B2.
7. a kind of preparation method of edible fungus liquid culture growth medium, it is characterised in that comprise the following steps：1）Culture medium is configured：By the sucrose in raw material, magnesium sulfate, potassium dihydrogen phosphate is soluble in water is sufficiently stirred for, until being dissolved into semi-finished product solution；Again by stirring and dissolving in the bean powder in the raw material, corn flour, the dusty yeast addition semi-finished product solution, medium liquid is brewed to；2）Culture medium is adjusted：The pH value of the medium liquid is adjusted with PH conditioning agents；3）Medium sterilization：The medium liquid is poured into fermentation tank, the sterilizing of at least 30 minutes is carried out in feeding sterilizing installation, in the case where nutrition is not lost, kill the bacterium in the medium liquid；4）Culture medium inoculated：After the completion of sterilizing, the medium liquid is cooled to 25 DEG C, accesses edible fungus species, and the liquid medium temperature is adjusted to after 20-22 DEG C, the medium liquid is carried out into aerobic culture, fermented and cultured obtains edible fungus species in 7-10 days.
8. the preparation method of edible fungi liquid strain according to claim 7, it is characterised in that：Step 3）In, when being sterilized to the medium liquid, sterilising temp is 121-125 DEG C, and pressure is 0.105-0.135Mpa.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108207493A (en) * | 2018-02-09 | 2018-06-29 | 山东省农业科学院农业资源与环境研究所 | A kind of grass corruption type edible fungus liquid culture growth medium, preparation method and application |
| CN108207492A (en) * | 2018-02-09 | 2018-06-29 | 山东省农业科学院农业资源与环境研究所 | A kind of Wood rot type edible fungus liquid culture growth medium, preparation method and application |
| CN112646726A (en) * | 2019-10-11 | 2021-04-13 | 孙傲 | Culture medium for black fungus fermentation, fermentation method and fermented beverage |
-
2015
- 2015-12-03 CN CN201510873397.XA patent/CN106831037A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108207493A (en) * | 2018-02-09 | 2018-06-29 | 山东省农业科学院农业资源与环境研究所 | A kind of grass corruption type edible fungus liquid culture growth medium, preparation method and application |
| CN108207492A (en) * | 2018-02-09 | 2018-06-29 | 山东省农业科学院农业资源与环境研究所 | A kind of Wood rot type edible fungus liquid culture growth medium, preparation method and application |
| CN112646726A (en) * | 2019-10-11 | 2021-04-13 | 孙傲 | Culture medium for black fungus fermentation, fermentation method and fermented beverage |
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