CN102381896B - Crab-flavor mushroom liquid strain medium formula and preparation method thereof - Google Patents
Crab-flavor mushroom liquid strain medium formula and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the field of bioengineering, and in particular relates to a medium and a preparation method thereof. A crab-flavor mushroom liquid strain medium formula comprises raw materials and an aqueous solution, wherein the raw materials comprise the following components by weight percent: 1.5%-2.5% of white granulated sugar, 0.1%-0.3% of bean pulp powder, 0.1%-0.3% of yeast extract powder, 0.05%-0.1% of monopotassium phosphate and 0.05%-0.1% of magnesium sulfate. The preparation method of the crab-flavor mushroom liquid strain medium formula comprises the following steps of medium preparation, regulation, sterilization and inoculating culture. Because the technical scheme is utilized, after the medium of the invention is used as the medium of crab-flavor mushroom liquid strains, the crab-flavor mushroom mycelium is rapid in growth, strong in activity and high in mycelium content; and the inoculated strain is good in germination rate, short in fermentation period and high in yield.
Description
Technical field
The present invention relates to bioengineering field, relate in particular to a kind of substratum and preparation method thereof.
Background technology
Crab mushroom has the advantages such as taste is fresher than flat mushroom, meat is thicker than sliding mushroom, matter is more tough than mushroom, excellent taste, in crab mushroom, contain and enrich VITAMIN and 17 seed amino acids, wherein Methionin, arginic content, higher than general mushroom class, contribute to teenager's intelligence development to increase, anticancer, reduction cholesterol.Crab mushroom also has unique crab fragrance, is one of world-renowned edible mushrooms.Crab mushroom is produced bacterial classification used solid spawn and liquid spawn, and liquid spawn has the advantages such as cost is low, a bacterium is fast, biological transformation ratio is high with respect to solid spawn, is the trend of Edible Fungi Industry Development technical renovation.And liquid spawn mainly relies on the nutrient that absorbs liquid nutrient medium to grow.Current existing crab-flavor mushroom liquid strain medium and preparation method exist and are prepared into that power is low, mycelial growth inadaptable, operation professional technique require high, do not possess the shortcomings such as versatility, cannot popularize in enormous quantities use.
Summary of the invention
The object of the present invention is to provide a kind of crab-flavor mushroom liquid strain medium formula, to solve the problems of the technologies described above.
Another object of the present invention is to provide a kind of preparation method of crab-flavor mushroom liquid strain medium, to solve the problems of the technologies described above.
Technical problem solved by the invention can realize by the following technical solutions:
Crab-flavor mushroom liquid strain medium formula, comprise raw material and the aqueous solution, described raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.
Described raw material also comprises Semen Maydis powder 0.2%-0.4%.
Described raw material preferably includes following part by weight component: white sugar 2%, bean cake powder 0.2%, Semen Maydis powder 0.3%, yeast soak powder 0.2%, potassium primary phosphate 0.07%, magnesium sulfate 0.08%, sorghum flour 0.1%-0.3%.
The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the crab mushroom mycelial growth that adopts the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Described raw material also comprises following part by weight component: ginkgo leaf powder leach liquor 0.01%-0.5%.The synthetic collagen protein ingredient rich content of yellow glucoside, amino acid and amino acid in Ginkgo Leaf, contributes to growing of yellow golden mushroom mycelium.
The preparation method of crab-flavor mushroom liquid strain medium, comprises the steps:
1) substratum configuration: by the white sugar in raw material, magnesium sulfate, potassium primary phosphate abundant stirring soluble in water, until dissolve;
Bean cake powder in raw material, Semen Maydis powder, yeast are soaked to powder and be added to the water stirring and dissolving, defoam;
The above-mentioned aqueous solution that contains white sugar, magnesium sulfate, potassium primary phosphate and the aqueous solution that contains bean cake powder, Semen Maydis powder, yeast and soak powder are mixed, make medium liquid;
2) adjust: the pH value of adjusting described medium liquid with hydrochloric acid;
3) sterilization: described medium liquid is carried out in disinfection equipment to germicidal treatment; In the situation that not losing nutrition, kill the bacterium in medium liquid.
4) inoculation culture: the medium liquid after germicidal treatment is cooled to below 25 degree in fermentor tank, access crab mushroom bacterial classification, regulates culture temperature 18～20 degree, and described fermentor tank passes into purify air and carries out aerobic cultivation, fermentation culture 5-8 days, can make crab mushroom liquid spawn.
Substratum preparation technology of the present invention is simple to operate, is applicable to batch production, normalizing operation.
Described raw material comprises following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, Semen Maydis powder 0.2%-0.4%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.
Described raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when the described medium liquid of allotment, adds described ginkgo leaf powder leach liquor, stirs, and contains described ginkgo leaf powder leach liquor in described medium liquid.Yeast soaks powder, can adopt yeast extract, as high-quality nitrogenous source.
During germicidal treatment, adopt high temperature and high pressure steam sterilization processing mode, described medium liquid is carried out to germicidal treatment.
Beneficial effect: owing to adopting technique scheme, after the substratum of crab mushroom liquid spawn, crab mushroom mycelial growth is fast, energetic, hyphae content is high using the present invention, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Accompanying drawing explanation
Fig. 1 is preparation method's of the present invention schema.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
Crab-flavor mushroom liquid strain medium formula, comprise raw material and the aqueous solution, raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.Raw material also comprises Semen Maydis powder 0.2%-0.4%.Raw material preferably includes following part by weight component: white sugar 2%, bean cake powder 0.2%, Semen Maydis powder 0.3%, yeast soak powder 0.2%, potassium primary phosphate 0.07%, magnesium sulfate 0.08%.The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the crab mushroom mycelial growth that adopts the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
Raw material also comprises following part by weight component: ginkgo leaf powder leach liquor 0.01%-0.5%.The synthetic collagen protein ingredient rich content of yellow glucoside, amino acid and amino acid in Ginkgo Leaf, contributes to growing of yellow golden mushroom mycelium.Raw material preferably includes following part by weight component: white sugar 1.5%, bean cake powder 0.1%, Semen Maydis powder 0.2%, yeast soak powder 0.2%, potassium primary phosphate 0.09%, magnesium sulfate 0.09%, straw powder 1%, ginkgo leaf powder leach liquor 0.4%, sorghum flour 0.1%-0.3%.
Bean cake powder, as slow effect nitrogenous source, provides nitrogen nutriment.Yeast soaks powder, and as quick-acting nitrogenous sources, Quick is for nitrogen nutriment.Be conducive to crab mushroom Fast Growth.And can effectively guarantee the growth quality of mycelia.Be conducive to mycelia balling-up, and balling-up shape is complete, is conducive to the growing state of visual determination mycelia.
With reference to Fig. 1, the preparation method of crab-flavor mushroom liquid strain medium, comprises the steps: the first step, substratum configuration: by the white sugar in raw material, magnesium sulfate, potassium primary phosphate abundant stirring soluble in water, until dissolve.Bean cake powder in raw material, Semen Maydis powder, yeast are soaked to powder and be added to the water stirring and dissolving, defoam.The above-mentioned aqueous solution that contains white sugar, magnesium sulfate, potassium primary phosphate and the aqueous solution that contains bean cake powder, Semen Maydis powder, yeast and soak powder are mixed, make medium liquid.Raw material when substratum configures in the first step is allocated according to following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, Semen Maydis powder 0.2%-0.4%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%.
Raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when allotment medium liquid, adds ginkgo leaf powder leach liquor, stirs, and contains ginkgo leaf powder leach liquor in medium liquid.
Second step, adjusts: the pH value of adjusting medium liquid with hydrochloric acid;
The 3rd step, sterilization: medium liquid is carried out in disinfection equipment to germicidal treatment; In the situation that not losing nutrition, kill the bacterium in medium liquid.During germicidal treatment, adopt high temperature and high pressure steam sterilization processing mode, medium liquid is carried out to germicidal treatment.
The 4th step, inoculation culture: the medium liquid after germicidal treatment is cooled to below 25 degree in fermentor tank to access crab mushroom bacterial classification, regulate culture temperature 18～20 degree, fermentor tank passes into purify air and carries out aerobic cultivation, and fermentation culture 5-8 days, can make crab mushroom liquid spawn.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (5)
1. crab-flavor mushroom liquid strain medium, comprise raw material and the aqueous solution, it is characterized in that, described raw material comprises following part by weight component: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%, ginkgo leaf powder leach liquor 0.01%-0.5%.
2. crab-flavor mushroom liquid strain medium according to claim 1, is characterized in that: described raw material also comprises Semen Maydis powder 0.2%-0.4%.
3. crab-flavor mushroom liquid strain medium according to claim 2, is characterized in that: described raw material comprises following part by weight component: white sugar 2%, bean cake powder 0.2%, Semen Maydis powder 0.3%, yeast soak powder 0.2%, potassium primary phosphate 0.07%, magnesium sulfate 0.08%, sorghum flour 0.1%-0.3%.
4. the preparation method of crab mushroom liquid spawn, is characterized in that, comprises the steps:
1) substratum configuration: by the white sugar in raw material, magnesium sulfate, potassium primary phosphate abundant stirring soluble in water, until dissolve;
Bean cake powder in raw material, Semen Maydis powder, yeast are soaked to powder and be added to the water stirring and dissolving, defoam;
The above-mentioned aqueous solution that contains white sugar, magnesium sulfate, potassium primary phosphate and the aqueous solution that contains bean cake powder, Semen Maydis powder, yeast and soak powder are mixed, make medium liquid;
2) adjust: the pH value of adjusting described medium liquid with hydrochloric acid;
3) sterilization: described medium liquid is carried out in disinfection equipment to germicidal treatment; In the situation that not losing nutrition, kill the bacterium in medium liquid;
4) inoculation culture: the medium liquid after germicidal treatment is cooled to below 25 degree in fermentor tank, access crab mushroom bacterial classification, regulates culture temperature 18～20 degree, and described fermentor tank passes into purify air and carries out aerobic cultivation, fermentation culture 5-8 days, can make crab mushroom liquid spawn;
Described raw material comprises following part by weight composition: white sugar 1.5%-2.5%, bean cake powder 0.1%-0.3%, Semen Maydis powder 0.2%-0.4%, yeast soak powder 0.1%-0.3%, potassium primary phosphate 0.05%-0.1%, magnesium sulfate 0.05%-0.1%;
Described raw material also comprises ginkgo leaf powder leach liquor 0.01%-0.5%, when the described medium liquid of allotment, adds described ginkgo leaf powder leach liquor, stirs.
5. the preparation method of crab mushroom liquid spawn according to claim 4, is characterized in that, during germicidal treatment, adopts high temperature and high pressure steam sterilization processing mode, and described medium liquid is carried out to germicidal treatment.
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| CN102964152A (en) * | 2012-08-30 | 2013-03-13 | 上海雪榕生物科技股份有限公司 | Culture medium formula and preparation method of white beech mushroom liquid spawn |
| CN102972208B (en) * | 2012-12-12 | 2015-01-07 | 天水众兴菌业科技股份有限公司 | Method for preparing edible mushroom liquefied strains |
| CN102976852A (en) * | 2012-12-12 | 2013-03-20 | 天水众兴菌业科技股份有限公司 | Water-soluble culture medium for edible fungus liquefaction strain |
| CN105837324B (en) * | 2016-03-28 | 2017-04-12 | 东莞市合心生物科技有限公司 | Hypsizigus marmoreus liquid strain medium and planting method |
| CN106047720A (en) * | 2016-06-27 | 2016-10-26 | 合肥福泉现代农业科技有限公司 | Red-date-peel-powder-based Hypsizygus marmoreus culture medium and Hypsizygus marmoreus liquid strain preparation method |
| CN112840962B (en) * | 2021-04-14 | 2022-08-23 | 江苏海洋大学 | Preparation method of Hypsizygus marmoreus liquid strain |
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| CN101293791A (en) * | 2008-06-13 | 2008-10-29 | 上海浦东天厨菇业有限公司 | Liquid bacterial culture medium for industrial preparation of hypsizygus marmoreus and preparation method thereof |
| CN101317527A (en) * | 2007-06-08 | 2008-12-10 | 上海丰科生物科技股份有限公司 | Process for preparing protospecies of white beech mushroom |
| CN101743843A (en) * | 2008-12-18 | 2010-06-23 | 上海丰科生物科技股份有限公司 | Method for cultivating brown crab taste mushroom |
| CN101891515A (en) * | 2009-05-18 | 2010-11-24 | 李畅 | Hypsizigus marmoreus compost |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101317527A (en) * | 2007-06-08 | 2008-12-10 | 上海丰科生物科技股份有限公司 | Process for preparing protospecies of white beech mushroom |
| CN101293791A (en) * | 2008-06-13 | 2008-10-29 | 上海浦东天厨菇业有限公司 | Liquid bacterial culture medium for industrial preparation of hypsizygus marmoreus and preparation method thereof |
| CN101743843A (en) * | 2008-12-18 | 2010-06-23 | 上海丰科生物科技股份有限公司 | Method for cultivating brown crab taste mushroom |
| CN101891515A (en) * | 2009-05-18 | 2010-11-24 | 李畅 | Hypsizigus marmoreus compost |
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