CN101643373A - Needle mushroom liquid spawn culture medium and preparation method thereof - Google Patents
Needle mushroom liquid spawn culture medium and preparation method thereof Download PDFInfo
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- CN101643373A CN101643373A CN200810012716A CN200810012716A CN101643373A CN 101643373 A CN101643373 A CN 101643373A CN 200810012716 A CN200810012716 A CN 200810012716A CN 200810012716 A CN200810012716 A CN 200810012716A CN 101643373 A CN101643373 A CN 101643373A
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Abstract
The invention discloses a needle mushroom liquid spawn culture medium and a preparation method thereof, which are characterized in that: according to the formulation of the culture medium, each litterof aqueous solution of the culture medium comprises 3 to 5 grams of soluble starch, 2 to 4 grams of glucose, 0.5 to 1 gram of peptone, 0.2 to 0.5 gram of calcium carbonate, 0.5 to 0.8 gram of magnesium sulfate, 1 to 2 millgrams of vitamin, 0.5 to 0.8 gram of monopotassium phosphate and 0.1 to 1 milliliter of polypropylene epoxy diglycidyl ether; and the preparation method comprises the steps of dissolving the components in water according to the formulation to prepare the culture medium, sterilizing culture solution, inoculating needle mushroom spawn into the culture solution after the sterilization is finished, adjusting culture temperature and introducing purified air for aerobic culture. The manufacturing method effectively solves the problems of the prior art, improves hyphae vitalityby 15 percent and stability by 30 percent, achieves a success rate of seed production up to 80 to 85 percent, and is simple in operation and suitable to be popularized.
Description
Technical field
The present invention relates to a kind of Edible Fungi method, particularly relate to a kind of needle mushroom liquid spawn culture medium and preparation method, belong to the technical field of edible fungi production in the biotechnology.
Background technology
Needle mushroom formal name used at school hair handle money bacterium is commonly called as structure bacterium, plain mushroom, dried mushroom etc., because of its stem is elongated, like Flos Hemerocallis, so claim needle mushroom, belong to Agaricales Tricholomataceae genus flammulina, needle mushroom fine and tender taste, soft cunning, taste is pleasant, and its protein content height is one of world-renowned edible mushrooms.Needle mushroom is produced used bacterial classification liquid spawn and solid spawn, and liquid spawn has low, the advantage that bacterium is fast, biological transformation ratio is high of cost with respect to solid spawn, will be the trend of mushroom industry Development Technology innovation.And liquid spawn why to grow be to absorb the fast reason of nutrient soon, therefore the key point of liquid spawn is that a rational liquid nutrient medium will be arranged, present existing needle mushroom liquid spawn culture medium and preparation method exist and are prepared into that power is low, mycelial growth is incompatible, the operation professional technique require high, do not possess versatility, can only be in the research institutions laboratory and the bigger factory of scale strength just can produce, can't reach general universal shortcoming.Particularly existing liquid nutrient medium does not consider that its mycelial growth decomposes the factor of nutrition that nutrient consumes, promptly only consider the nutritional needs of needle mushroom growth, ignored the nutrition that is consumed in absorbing the nutrient decomposition course, it is bad or the wear out problem of regression of mycelia occurs to late stage of culture mycelial growth therefore can to occur.For example, publication number is a kind of " golden mushroom factory-production of liquid bacterial culture medium and preparation method thereof " that the Chinese invention patent application of CN101041808 provides, and it is characterized in that containing in every liter of substratum aqueous solution: soyabean expeller powder 3g～10g, sucrose 20g～30g, sal epsom 0.3g～1g, potassium primary phosphate 0.5g～2g, Xylo-Mucine 0g～2g, bubble enemy 0.1ml～0.5ml.Its preparation method: take by weighing sucrose, sal epsom, potassium primary phosphate and Xylo-Mucine by proportioning, add boiling water behind the mixing and be stirred to dissolving fully; Take by weighing bean cake powder, defoam after the adding cold water stirring and dissolving; The solution mixing that makes is made mixed solution, be settled to volume required; With adding the bubble enemy after the hydrochloric acid adjustment pH of mixed value; High pressure steam sterilization after the packing mixed solution.The liquid nutrient medium that this technical scheme provides is the same with existing other liquid nutrient medium, not only will not consume nutrition and take into account, and adopt sucrose, some nutrients that can not directly absorb of analysis for soybean powder, causes the not enough mycelial growth of later stage nutrient bad; And complicated process of preparation: synthesize by prescription earlier, reinstall the Autoclave sterilization through the processing of precooking, pack into then triangular flask or fermentor cultivation, the per pass operation is all very loaded down with trivial details, add nutritious bacterium, the fungal contamination of in the process of packing back and forth, being subjected to easily of nutrient solution, reduced production of hybrid seeds success ratio.
Summary of the invention
The objective of the invention is to overcome the prior art above shortcomings, improve, a kind of liquid spawn culture medium of optimum needle mushroom growth is provided and can reaches and popularize general preparation technology by research.The liquid spawn culture medium that the present invention provides fully takes into account the factor that its mycelial growth decomposes nutrition that nutrient consumes, not only consider the nutritional needs of needle mushroom growth, also consider the nutrition that in absorbing the nutrient decomposition course, is consumed, substantially can supply with needle mushroom growth and directly absorb, thereby it is bad or the wear out problem of regression of mycelia occurs to late stage of culture to have solved mycelial growth; The preparation technology that the present invention provides handles raw material, sterilize, cultivate and carry out in an equipment, reduce the packing link, original relatively preparation technology has not only reduced operation easier, in fermentation culture, carry out simultaneously the temperature control aerobic and cultivate, also improve production of hybrid seeds success ratio greatly and strengthened the mycelia activity.
The technical scheme that the present invention provides is: this needle mushroom liquid spawn culture medium is characterized in that the component of substratum is:
Zulkovsky starch 3g～5g, glucose 2g～4g, peptone 0.5g～1g,
Lime carbonate 0.2g～0.5g, sal epsom 0.5g～0.8g, VITAMIN 1mg～2mg,
Potassium primary phosphate 0.5g～0.8g, poly-third Synthesis of Oligo Ethylene Glycol 0.1ml～1ml
1 liter in water.
Described VITAMIN is one or more in VB11, vitamin B12, the VITMAIN B1.
The preparation method of this flammulina velutipes liquid strains that the present invention provides is:
(1) substratum preparation mixes and pours biological fermentation equipment into Zulkovsky starch, glucose, peptone, lime carbonate, VITAMIN, sal epsom, potassium primary phosphate, poly-third Synthesis of Oligo Ethylene Glycol are soluble in water by prescription;
(2) medium sterilization is sterilized culture medium solution in equipment, keep 122 ℃～126 ℃ of temperature, 1.2 kilograms～1.5 kilograms/cm of pressure
2Following sterilization at least 30 minutes keeps not losing under the situation of nutrition with the culture medium solution sterilization thoroughly;
(3) the temperature control aerobic fermentation is cultivated, after sterilization is finished, culture medium solution is cooled to the needle mushroom of access below 25 ℃ bacterial classification, regulates 19 ℃-23 ℃ of culture temperature, feeding purifies air and carries out the aerobic cultivation, can make good flammulina velutipes liquid strains in fermentation culture 3-6 days.
In the culture medium solution process for preparation, each component of substratum (dewater outer) but the addition sequence randomize, described biological fermentation equipment is a conventional equipment.
Compared with prior art, the invention has the beneficial effects as follows: effectively solved the problem that prior art exists, from the upgrowth situation of needle mushroom, can improve 15% mycelia vigor, stability can improve 30%; On preparation technology, original technology production of hybrid seeds success ratio is 50%-65%, and the present invention can reach 80%-85%.And simple more suitable popularizing in the operation.
Embodiment
Embodiment 1:
Take by weighing by prescription: Zulkovsky starch 4g, glucose 2g, peptone 0.5g, lime carbonate 0.2g, sal epsom 0.5g, potassium primary phosphate 0.5g, VITAMIN 1mg, the poly-third Synthesis of Oligo Ethylene Glycol 0.1ml are dissolved in and mix in 1 premium on currency and pour biological fermentation equipment into and sterilize, keep 126 ℃ of temperature, 1.5 kilograms/cm of pressure
2Under sterilized 30-40 minute, under the situation of not losing nutrition that medium sterilization is thorough, after the sterilization, insert the needle mushroom bacterial classification when being cooled to nutrient solution below 25 ℃, 19 ℃ of-23 ℃ of feedings of adjusting culture temperature purify air and carry out the aerobic cultivation, can make good flammulina velutipes liquid strains in fermentation culture 3-6 days.
Embodiment 2:
Take by weighing by prescription: Zulkovsky starch 3g, glucose 4g, peptone 0.7g, lime carbonate 0.4g, sal epsom 0.3g, potassium primary phosphate 0.7g, VITAMIN 1.2mg, poly-third Synthesis of Oligo Ethylene Glycol of 0.1ml are dissolved in mixes in 1 premium on currency and pours biological fermentation equipment into and sterilize, at 122 ℃ of temperature, pressure at 1.2 kilograms/cm
2Under sterilized 40-60 minute.Sterilization back temperature is cooled to and inserts the needle mushroom bacterial classification below 25 ℃, and 19 ℃ of-23 ℃ of feedings of adjusting culture temperature purify air and carry out the aerobic cultivation, can make good flammulina velutipes liquid strains in fermentation culture 3-6 days.
Claims (3)
1. needle mushroom liquid spawn culture medium, the component that it is characterized in that substratum is: Zulkovsky starch 3g～5g, glucose 2g～4g, peptone 0.5g～1g, lime carbonate 0.2g～0.5g, sal epsom 0.5g～0.8g, VITAMIN 1mg～2mg potassium primary phosphate 0.5g～0.8g, 1 liter of poly-third Synthesis of Oligo Ethylene Glycol 0.1ml～1ml water.
2. according to the described needle mushroom liquid spawn culture medium of claim 1, it is characterized in that described VITAMIN is one or more in VB11, vitamin B12, the VITMAIN B1.
3. the preparation method of a flammulina velutipes liquid strains is characterized in that including following steps:
(1) substratum preparation mixes and pours biological fermentation equipment into Zulkovsky starch, glucose, peptone, lime carbonate, VITAMIN, sal epsom, potassium primary phosphate, poly-third Synthesis of Oligo Ethylene Glycol are soluble in water by prescription;
(2) medium sterilization is sterilized culture medium solution in equipment, keep 122 ℃～126 ℃ of temperature, 1.2 kilograms～1.5 kilograms/cm of pressure
2Following sterilization at least 30 minutes keeps not losing under the situation of nutrition with the culture medium solution sterilization thoroughly;
(3) the temperature control aerobic fermentation is cultivated, after sterilization is finished, culture medium solution is cooled to the needle mushroom of access below 25 ℃ bacterial classification, regulates 19 ℃-23 ℃ of culture temperature, feeding purifies air and carries out the aerobic cultivation, can make good flammulina velutipes liquid strains in fermentation culture 3-6 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810012716A CN101643373A (en) | 2008-08-08 | 2008-08-08 | Needle mushroom liquid spawn culture medium and preparation method thereof |
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| CN200810012716A CN101643373A (en) | 2008-08-08 | 2008-08-08 | Needle mushroom liquid spawn culture medium and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102349416A (en) * | 2011-09-08 | 2012-02-15 | 湖南农业大学 | Simple method for preparing liquid needle mushroom spawn |
| CN102910949A (en) * | 2011-08-06 | 2013-02-06 | 天水众兴菌业科技股份有限公司 | Flammulina velutipes liquid strain culture medium and preparation method thereof |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN105638232A (en) * | 2014-11-13 | 2016-06-08 | 上海市农业科学院 | Domestic fungus liquid spawn culture method and medium thereof |
| CN105733967A (en) * | 2016-05-05 | 2016-07-06 | 盐城工学院 | Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof |
| CN107628885A (en) * | 2017-11-06 | 2018-01-26 | 酒泉百山农业开发有限公司 | method for cultivating mushroom |
| CN107937329A (en) * | 2017-11-21 | 2018-04-20 | 石家庄学院 | A kind of method for improving liquid spawn vigor |
-
2008
- 2008-08-08 CN CN200810012716A patent/CN101643373A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102910949A (en) * | 2011-08-06 | 2013-02-06 | 天水众兴菌业科技股份有限公司 | Flammulina velutipes liquid strain culture medium and preparation method thereof |
| CN102349416A (en) * | 2011-09-08 | 2012-02-15 | 湖南农业大学 | Simple method for preparing liquid needle mushroom spawn |
| CN102349416B (en) * | 2011-09-08 | 2012-09-05 | 湖南农业大学 | Simple method for preparing golden mushroom liquid strain |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN103242080B (en) * | 2013-05-20 | 2014-08-13 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN105638232A (en) * | 2014-11-13 | 2016-06-08 | 上海市农业科学院 | Domestic fungus liquid spawn culture method and medium thereof |
| CN105733967A (en) * | 2016-05-05 | 2016-07-06 | 盐城工学院 | Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof |
| CN107628885A (en) * | 2017-11-06 | 2018-01-26 | 酒泉百山农业开发有限公司 | method for cultivating mushroom |
| CN107937329A (en) * | 2017-11-21 | 2018-04-20 | 石家庄学院 | A kind of method for improving liquid spawn vigor |
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Open date: 20100210 |