CN102910949A - Flammulina velutipes liquid strain culture medium and preparation method thereof - Google Patents

Flammulina velutipes liquid strain culture medium and preparation method thereof Download PDF

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Publication number
CN102910949A
CN102910949A CN2011102320706A CN201110232070A CN102910949A CN 102910949 A CN102910949 A CN 102910949A CN 2011102320706 A CN2011102320706 A CN 2011102320706A CN 201110232070 A CN201110232070 A CN 201110232070A CN 102910949 A CN102910949 A CN 102910949A
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China
Prior art keywords
juice
culture medium
raw
water
peptone
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Pending
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CN2011102320706A
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Chinese (zh)
Inventor
陶军
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TIANSHUI ZHONGXING BIO-TECHNOLOGY Co Ltd
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TIANSHUI ZHONGXING BIO-TECHNOLOGY Co Ltd
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Priority to CN2011102320706A priority Critical patent/CN102910949A/en
Publication of CN102910949A publication Critical patent/CN102910949A/en
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Abstract

The invention discloses a flammulina velutipes liquid strain culture medium and a preparation method thereof; and the culture medium comprises the following raw materials in percentage by weight: 0.25-0.35 % of peptone, 1.2-1.8 % of glucose, 0.8-1.2 % of KH2PO4, 0.03-0.07 % of MgSO4, 0.008-0.012 % of VB, 2-3 % of corn flour, 1.5-2.5 % of bran and the balance of water; and the preparation method comprises the following steps of: 1) preparation of raw materials: weighing the raw materials according to raw material composition, respectively adding 10 times of water to the brans and the corn flour so as to boil up the brans and the corn flour for 20 minutes, and filtering the boiled materials, and taking a juice for future use; 2) preparation of the culture medium: mixing a corn flour juice and a bran juice, dissolving KH2PO4, MgSO4, the peptone and glucose in the water, adding the dissolved materials to the mixed juice, crushing the VB1 and adding the crushed VB1 to the mixed juice; and 3) sterilization of the culture medium: filling the prepared culture medium into biological fermentation equipment, sterilizing and cooling the culture medium so as to obtain the flammulina velutipes liquid strain culture medium.

Description

Needle mushroom liquid spawn culture medium and preparation method thereof
Technical field
The present invention relates to the Edible Fungi technology, be specifically related to a kind of needle mushroom liquid spawn culture medium and preparation method thereof, belong to the Edible Fungi field in the biotechnology.
Background technology
In the prior art, solid spawn is used in the production of needle mushroom mostly, uses solid spawn to have following shortcoming: the production cycle is long, strain quality is difficult to control, the fruiting reguarity is difficult to control, it is slow to send out bacterium speed; Began in recent years the research that someone carries out the liquid fungus seed culture technique, and the strain cultivation key problem in technology is that a rational substratum will be arranged, existing needle mushroom liquid spawn culture medium and preparation method thereof exists and is prepared into the shortcoming that power is low, mycelial growth inadaptable, the requirement of operation professional technique is high, do not possess versatility, can only in Laboratory Production, can't popularize.
Summary of the invention
Technical problem to be solved by this invention provides a kind of needle mushroom liquid spawn culture medium and preparation method thereof.
For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of needle mushroom liquid spawn culture medium, it is comprised of the raw material of following weight per-cent: peptone 0.25~0.35%, glucose 1.2~1.8%, KH 2PO 40.8~1.2%, MgSO 40.03~0.07%, VB 10.008~0.012%, Semen Maydis powder 2~3%, wheat bran 1.5~2.5%, surplus is water; Its preparation method comprises the steps: 1) raw material prepares to form by described raw material and takes by weighing raw material, the wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use, the Semen Maydis powder that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use; 2) the substratum preparation mixes Semen Maydis powder juice and wheat bran juice, with KH 2PO 4, MgSO 4Add behind the molten water in the described mixing juice, will add in the described mixing juice, again with VB behind peptone, the molten water of glucose again 1Pulverize in the described mixing juice of rear adding, adjust pH value between 6~6.5 with hydrochloric acid or the sodium hydroxide of concentration 10%; 3) medium sterilization keeps 122~126 ℃ of temperature, pressure 1.2~1.5Kg/cm with the described substratum that the prepares biological fermentation equipment of packing into 2Then lower sterilization 30~35 minutes cools off for subsequent use.
Preferably, described substratum is comprised of the raw material of following weight per-cent: peptone 0.3%, glucose 1.5%, KH 2PO 41%, MgSO 40.05%, VB 10.01%, Semen Maydis powder 2.5%, wheat bran 2%, surplus is water; Its preparation method preceding method is identical, repeats no more.
The invention has the beneficial effects as follows, the prescription that substratum adopts is reasonable, nutritious, can satisfy the culture propagation nutritional need, raw material sources are abundant, and cheap, preparation technology is simple to operate, be applicable to the batch production operation, from the needle mushroom production status, can improve 15% mycelia vigor, stability can improve 30%, on preparation technology, production of hybrid seeds success ratio of the present invention can reach 75~82%.
Embodiment
Embodiment 1
A kind of needle mushroom liquid spawn culture medium, it is comprised of the raw material of following weight per-cent: peptone 0.25, glucose 1.2%, KH 2PO 40.8%, MgSO 40.03%, VB 10.012%, Semen Maydis powder 3%, wheat bran 2.5%, surplus is water; Its preparation method comprises the steps: 1) raw material prepares to form by described raw material and takes by weighing raw material, the wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use, the Semen Maydis powder that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use; 2) the substratum preparation mixes Semen Maydis powder juice and wheat bran juice, with KH 2PO 4, MgSO 4Add behind the molten water in the described mixing juice, will add in the described mixing juice, again with VB behind peptone, the molten water of glucose again 1Pulverize in the described mixing juice of rear adding, adjusting pH value with the hydrochloric acid of concentration 10% or sodium hydroxide is 6.5; 3) medium sterilization keeps 122 ℃ of temperature, pressure 1.2Kg/cm with the described substratum that the prepares biological fermentation equipment of packing into 2Then lower sterilization 30~35 minutes cools off for subsequent use.
Embodiment 2
A kind of needle mushroom liquid spawn culture medium, it is comprised of the raw material of following weight per-cent: peptone 0.35%, glucose 1.8%, KH 2PO 41.2%, MgSO 40.07%, VB 10.008%, Semen Maydis powder 2%, wheat bran 1.5%, surplus is water; Its preparation method comprises the steps: 1) raw material prepares to form by described raw material and takes by weighing raw material, the wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use, the Semen Maydis powder that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use; 2) the substratum preparation mixes Semen Maydis powder juice and wheat bran juice, with KH 2PO 4, MgSO 4Add behind the molten water in the described mixing juice, will add in the described mixing juice, again with VB behind peptone, the molten water of glucose again 1Pulverize in the described mixing juice of rear adding, adjusting pH value with the hydrochloric acid of concentration 10% or sodium hydroxide is 6; 3) medium sterilization keeps 126 ℃ of temperature, pressure 1.5Kg/cm with the described substratum that the prepares biological fermentation equipment of packing into 2Then lower sterilization 30~35 minutes cools off for subsequent use.
Embodiment 3
A kind of needle mushroom liquid spawn culture medium, it is comprised of the raw material of following weight per-cent: peptone 0.3%, glucose 1.5%, KH 2PO 41%, MgSO 40.05%, VB 10.01%, Semen Maydis powder 2.5%, wheat bran 2%, surplus is water; Its preparation method comprises the steps: 1) raw material prepares to form by described raw material and takes by weighing raw material, the wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use, the Semen Maydis powder that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use; 2) the substratum preparation mixes Semen Maydis powder juice and wheat bran juice, with KH 2PO 4, MgSO 4Add behind the molten water in the described mixing juice, will add in the described mixing juice, again with VB behind peptone, the molten water of glucose again 1Pulverize in the described mixing juice of rear adding, adjusting pH value with the hydrochloric acid of concentration 10% or sodium hydroxide is being 6.2; 3) medium sterilization keeps 122 ℃ of temperature, pressure 1.5Kg/cm with the described substratum that the prepares biological fermentation equipment of packing into 2Then lower sterilization 30~35 minutes cools off for subsequent use.
Embodiment 4
A kind of needle mushroom liquid spawn culture medium, it is comprised of the raw material of following weight per-cent: peptone 0.28%, glucose 1.3%, KH 2PO 40.9%, MgSO 40.06%, VB 10.009%, Semen Maydis powder 2.8%, wheat bran 2.3%, surplus is water; Its preparation method comprises the steps: 1) raw material prepares to form by described raw material and takes by weighing raw material, the wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use, the Semen Maydis powder that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use; 2) the substratum preparation mixes Semen Maydis powder juice and wheat bran juice, with KH 2PO 4, MgSO 4Add behind the molten water in the described mixing juice, will add in the described mixing juice, again with VB behind peptone, the molten water of glucose again 1Pulverize in the described mixing juice of rear adding, adjusting pH value with the hydrochloric acid of concentration 10% or sodium hydroxide is 6.3; 3) medium sterilization keeps 125 ℃ of temperature, pressure 1.3Kg/cm with the described substratum that the prepares biological fermentation equipment of packing into 2Then lower sterilization 30~35 minutes cools off for subsequent use.

Claims (3)

1. a needle mushroom liquid spawn culture medium is characterized in that, it is comprised of the raw material of following weight per-cent: peptone 0.25~0.35%, glucose 1.2~1.8%, KH 2PO 40.8~1.2%, MgSO 40.03~0.07%, VB 10.008~0.012%, Semen Maydis powder 2~3%, wheat bran 1.5~2.5%, surplus is water.
2. needle mushroom liquid spawn culture medium according to claim 1 is characterized in that, described substratum is comprised of the raw material of following weight per-cent: peptone 0.3%, glucose 1.5%, KH 2PO 41%, MgSO 40.05%, VB 10.01%, Semen Maydis powder 2.5%, wheat bran 2%, surplus is water.
3. the preparation method of a claim 1 or 2 described needle mushroom liquid spawn culture mediums is characterized in that, described method comprises the steps:
1) raw material is prepared to form by described raw material and is taken by weighing raw material, and the wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, get juice for subsequent use, the Semen Maydis powder that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, with 6~7 layers of filtered through gauze, gets juice for subsequent use;
2) the substratum preparation mixes Semen Maydis powder juice and wheat bran juice, with KH 2PO 4, MgSO 4Add behind the molten water in the described mixing juice, will add in the described mixing juice, again with VB behind peptone, the molten water of glucose again 1Pulverize in the described mixing juice of rear adding, adjust pH value between 6~6.5 with hydrochloric acid or the sodium hydroxide of concentration 10%;
3) medium sterilization keeps 122~126 ℃ of temperature, pressure 1.2~1.5Kg/cm with the described substratum that the prepares biological fermentation equipment of packing into 2Then lower sterilization 30~35 minutes cools off for subsequent use.
CN2011102320706A 2011-08-06 2011-08-06 Flammulina velutipes liquid strain culture medium and preparation method thereof Pending CN102910949A (en)

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Publication number Priority date Publication date Assignee Title
CN103214294A (en) * 2013-03-08 2013-07-24 湖北富士峰生物科技有限公司 Secondary fruiting nutrient solution for golden mushroom and preparation method
CN104838894A (en) * 2015-06-05 2015-08-19 电白中茂生物科技有限公司 Culture method for white needle mushroom segment form liquid strain
CN105638232A (en) * 2014-11-13 2016-06-08 上海市农业科学院 Domestic fungus liquid spawn culture method and medium thereof
CN105733967A (en) * 2016-05-05 2016-07-06 盐城工学院 Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103214294A (en) * 2013-03-08 2013-07-24 湖北富士峰生物科技有限公司 Secondary fruiting nutrient solution for golden mushroom and preparation method
CN103214294B (en) * 2013-03-08 2014-08-27 湖北富士峰生物科技有限公司 Secondary fruiting nutrient solution for golden mushroom and preparation method
CN105638232A (en) * 2014-11-13 2016-06-08 上海市农业科学院 Domestic fungus liquid spawn culture method and medium thereof
CN104838894A (en) * 2015-06-05 2015-08-19 电白中茂生物科技有限公司 Culture method for white needle mushroom segment form liquid strain
CN105733967A (en) * 2016-05-05 2016-07-06 盐城工学院 Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof

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