CN1837354A - Ganoderma lucidum hypha powder or ganoderma lucidum tea and dual fermentation process thereof - Google Patents
Ganoderma lucidum hypha powder or ganoderma lucidum tea and dual fermentation process thereof Download PDFInfo
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- CN1837354A CN1837354A CN 200610035009 CN200610035009A CN1837354A CN 1837354 A CN1837354 A CN 1837354A CN 200610035009 CN200610035009 CN 200610035009 CN 200610035009 A CN200610035009 A CN 200610035009A CN 1837354 A CN1837354 A CN 1837354A
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Abstract
The invention discloses a lucid ganoderma hypha powder or lucid ganoderma tea and dual ferment technology, which comprises the following parts: lucid ganoderma mother kind, liquid hypha and ferment culture medium, wherein the lucid ganoderma mother kind adapts the improved PDA culture medium; the method adapts traditional fungal lucid ganoderma and high-activity yeast to proceed dual ferment based on main raw material of good-quality soybean or tea, which proceeds oxygen-enriched ferment for 1-4 h at 20-40 deg.c; the material is deactivated for 40-120 min at 0.08-0.15Mpa, which is switched-in the lucid ganoderma hypha to ferment for 20-40 days; when the hypha is dried and grinded, the product is formed. The invention displays high-product rate, which reduces the cost.
Description
Technical field:
The present invention relates to fungi ganoderma lucidum prod and zymotechnique flow process thereof.Specifically be fermentation, realize the efficient conversion of glossy ganoderma by high-activity yeast bacterium and glossy ganoderma.
Background technology:
Glossy ganoderma has a large amount of artificial cultures as the traditional a kind of medicinal fungi of China in China, production form comprise sporophore, spore powder with and deep processed product.Begin to utilize fermentation engineering to obtain mycelium from the seventies and make suitable formulation, fermentation process mainly is to adopt deep liquid to cultivate.In recent years, the tealeaves of employing also being arranged as fermented substrate, is a kind of novel biological fermentation tea.The shortcoming that the above-mentioned mode of production exists has: the one, and glossy ganoderma entity, spore powder production cycle are long, and quality product is uneven; The 2nd, using liquid submerged fermentation technology is produced mycelium, and meta-bolites is single, and yield rate is low, the production cost height; The 3rd, the solid-state fermentation technology of substance fails to make mineral substance to organise fully, and the meta-bolites of glossy ganoderma is not discharged effectively, and output is low, moreover the auxiliary material consumption is big.
Summary of the invention:
In order to overcome the shortcoming of prior art, the purpose of this invention is to provide and a kind ofly utilize high-activity yeast and glossy ganoderma to retransmit ferment technology again to produce Ganoderma lucidum mycelium powder or Ganoderma tea, so that glossy ganoderma is realized efficient the conversion, its meta-bolites is discharged fully, and have the output height, auxiliary material consumption is few, and meta-bolites is abundant, the yield rate height, the advantage that production cost is low.
In order to achieve the above object, the present invention by the following technical solutions: Ganoderma lucidum mycelium powder or Ganoderma tea comprise the female kind of glossy ganoderma, liquid spawn and fermention medium, the PDA substratum of glossy ganoderma mother culture media employing improvement; Prescription of liquid spawn and content are: glucose 2.0-4.0%, sucrose 0.8-2.0%, peptone 0.25-1.0%, yeast extract 0.2-0.8%, potassium primary phosphate KH
2PO
40.12-0.25%, sal epsom MgSo
40.02-0.12%, agar powder 0.06-0.15%, VITMAIN B1 VB
10.002-0.008%, defoamer 0.06-0.15%, pH value: nature; The prescription and the content of supporting base are joined in fermentation: soybean/tealeaves 68-80%, broken corn 8-18%, high-activity yeast 1.0-2.0%, glucose 2-7%, sucrose 3-9%, lime carbonate CaCO
30.8-1.6%, potassium primary phosphate KH
2PO
40.4-1.6%, sal epsom MgSO
40.3-0.9, zinc sulfate ZnSO
47H
2O0.12-0.25%, Sodium Selenite Na
2SeO
30.008-0.15%, and an amount of water.
The complete processing of Ganoderma lucidum mycelium powder or Ganoderma tea is: adopt tradition to carry out double fermentation with fungi glossy ganoderma and high-activity yeast, being in the raw material of major ingredient with high quality soybean or tealeaves, is to carry out oxygen enrichment fermentation 1-4 hour under 20 ℃-40 ℃ with high-activity yeast in room temperature; After pressure is to carry out 40-120 minute deactivation under the 0.08-0.15Mpa, insert the ganoderma lucidum liquid bacterial classification again and carried out the aerobic pure-blood ferment 20-40 days, treat that mycelium covers with technologies such as back drying, pulverizing and forms Ganoderma lucidum mycelium powder or Ganoderma teas.
The complete processing of Ganoderma lucidum mycelium powder or Ganoderma tea, comprise female cultivation, the preparation of liquid spawn and the preparation of fermention medium of planting of glossy ganoderma, it is substratum that the glossy ganoderma mother culture media adopts improvement PDA, divide the threading test tube with prepared culture medium, after sterilizing, inoculate and cultivating, both can obtain that glossy ganoderma is female to be planted; The preparation method of liquid spawn: obtained the one grade fermemtation bacterial classification before this, take by weighing in proportion after the raw material of liquid fermentation medium removed defoamer, put into after the water heating for dissolving triangular flask packing then with 100-2000ml, the liquid amount of each triangular flask is 50-1000ml, in temperature is 121-127 ℃, pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, treat to begin inoculation after its cooling, carry out shaking culture again after waiting its kind ferfas silk to sprout, the rotating speed of vibrating machine remains on 100-200 rev/min, cultivates and can obtain the one grade fermemtation kind after 4-7 days; The preparation of second order fermentation bacterial classification: employing eats or medicinal fungi liquid spawn deep layer incubator carries out fermentation culture, put into the water dissolving back that is mixed after raw material in the liquid fermentation medium prescription taken by weighing in proportion and add incubator, in temperature is 121-127 ℃, pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, the one grade fermemtation bacterial classification is inserted in the cooling back, carry out aerated culture then, can obtain the second order fermentation bacterial classification in 6-8 days, the second order fermentation bacterial classification can be used for solid state fermentation.The preparation of fermention medium: in soybean: water=ratio took by weighing soybean in 1.0: 2.0, had measured water, earlier with Sodium Selenite Na
2SeO
3Soluble in water, again soybean bubble is opened and inhaled permeablely, drain away the water after 3-10 hour, stir after putting into other raw material again, add high-activity yeast at last.The pH value keeps nature, is to carry out oxygen enrichment fermentation 1-3 hour under 20 ℃-40 ℃ in room temperature; The solid fermentation container of then above-mentioned mixing raw material being packed into; In temperature is 131-127 ℃, and pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, and the every bottle graft in natural step-down cooling back is gone into the ganoderma lucidum liquid bacterial classification; Be that 20 ℃-35 ℃, relative air humidity are condition bottom fermentation 20-40 days of 50-65% in temperature then, when mycelium covers with bottle, stop fermentation; It is under 60-80 ℃ in temperature that the back is finished in fermentation, carries out 10-18 hour oven dry processing, until its water content≤5%; Dried tunning is pulverized, and granularity is a standard to cross the 60-100 order.
The raw material of fermention medium all adopts food grade materials.
The fungi glossy ganoderma can be adopted red sesame, purple sesame, black sesame, blue or green sesame, Bai Zhi, rainbow conk or yellow sesame.
Advantage of the present invention is through saccharomycetic conversion, enrichment, absorption, auxiliary material is organised, again through the biological fermentation of glossy ganoderma, the product nutritive ingredient height that forms: protein 38.8%, hydrolysis total amino acid 31.5g/100g, polysaccharide 3.96%, calcium 0.81%, iron 0.0054%, zinc 0.044%, selenium 90.9mg/kg.Realize the accumulation of effectively conversion and meta-bolites, and can make glossy ganoderma realize efficient the conversion, meta-bolites is discharged fully, and has the output height, and auxiliary material consumption is few, and meta-bolites is abundant, yield rate height, the advantage that production cost is low.
Embodiment:
The invention will be further described below in conjunction with embodiment.
Principle of the present invention is to adopt tradition to carry out double fermentation with fungi glossy ganoderma and high-activity yeast, is being in the raw material of major ingredient with high quality soybean or tealeaves, is to carry out oxygen enrichment fermentation 1-4 hour under 20 ℃-40 ℃ with high-activity yeast in room temperature; After pressure is to carry out 40-120 minute deactivation under the 0.08-0.15Mpa, insert the ganoderma lucidum liquid bacterial classification again and carried out the aerobic pure-blood ferment 20-40 days, treat that mycelium covers with technologies such as back drying, pulverizing and forms Ganoderma lucidum mycelium powder or Ganoderma teas.
Embodiment one, adopt red sesame, mother culture media adopts the PDA substratum of improvement; Prescription of liquid spawn and content are: 2 parts of glucose, 0.8 part of sucrose, 0.3 part of peptone, 0.5 part of yeast extract, potassium primary phosphate KH
2PO
40.2 part, sal epsom MgSo
40.1 part, 0.1 part of agar powder, VITMAIN B1 VB
10.004 part, 0.08 part of defoamer, pH value: nature; The prescription and the content of supporting base are joined in fermentation: 70 parts of soybean/tealeaves, 10 parts of broken corns, 1.5 parts of high-activity yeasts, 4 parts of glucose, 5 parts of sucrose, lime carbonate CaCO
30.8 part, potassium primary phosphate KH
2PO
41 part, sal epsom MgSO
40.5 part, zinc sulfate ZnSO
47H
2O0.2 part, Sodium Selenite Na
2SeO
30.08 part, and an amount of water.
It is substratum that red sesame mother culture media adopts improvement PDA, prepared culture medium is divided put into test tube, after sterilizing, inoculate and cultivating, obtains that the cloud glossy ganoderma is female to be planted; The preparation method of liquid spawn: obtained the one grade fermemtation bacterial classification before this, take by weighing by part after the raw material of liquid fermentation medium removed defoamer, put into after the water heating for dissolving triangular flask packing then with 100-2000ml, the liquid amount of each triangular flask is 50-1000ml, in temperature is 121-127 ℃, pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, treat to begin inoculation after its cooling, carry out shaking culture again after waiting its kind ferfas silk to sprout, the rotating speed of vibrating machine remains on 100-200 rev/min, cultivates and can obtain the one grade fermemtation kind after 4-7 days; The preparation of second order fermentation bacterial classification: employing eats or medicinal fungi liquid spawn deep layer incubator carries out fermentation culture, put into the water dissolving back that is mixed after raw material in the liquid fermentation medium prescription taken by weighing in proportion and add incubator, in temperature is 121-127 ℃, pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, the one grade fermemtation bacterial classification is inserted in the cooling back, carry out aerated culture then, can obtain the second order fermentation bacterial classification in 6-8 days, the second order fermentation bacterial classification can be used for solid state fermentation.The preparation of fermention medium: in soybean: water=ratio took by weighing soybean in 1.0: 2.0, had measured water, earlier with Sodium Selenite Na
2SeO
3Soluble in water, again soybean bubble is opened and inhaled permeablely, drain away the water after 3-10 hour, stir after putting into other raw material again, add high-activity yeast at last.The pH value keeps nature, is to carry out oxygen enrichment fermentation 1-3 hour under 20 ℃-40 ℃ in room temperature; The solid fermentation container of then above-mentioned mixing raw material being packed into; In temperature is 121-127 ℃, and pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, and the every bottle graft in natural step-down cooling back is gone into the ganoderma lucidum liquid bacterial classification; Be that 20 ℃-35 ℃, relative air humidity are condition bottom fermentation 20-40 days of 50-65% in temperature then, when mycelium covers with bottle, stop fermentation; It is under 60-80 ℃ in temperature that the back is finished in fermentation, carries out 10-18 hour oven dry processing, until its water content≤5%; Dried tunning is pulverized, and granularity is a standard to cross the 60-100 order.The raw material of fermention medium all adopts food grade materials.
The fungi glossy ganoderma also can be adopted purple sesame, black sesame, blue or green sesame, Bai Zhi, rainbow conk or yellow sesame.
Claims (5)
1, Ganoderma lucidum mycelium powder or Ganoderma tea comprise the female kind of glossy ganoderma, liquid spawn and fermention medium, it is characterized in that the glossy ganoderma mother culture media adopts the PDA substratum of improvement; Prescription of liquid spawn and content are: glucose 2.0-4.0%, sucrose 0.8-2.0%, peptone 0.25-1.0%, yeast extract 0.2-0.8%, potassium primary phosphate KH
2PO
40.12-0.25%, sal epsom MgSo
40.02-0.12%, agar powder 0.06-0.15%, VITMAIN B1 VB
10.002-0.008%, defoamer 0.06-0.15%, pH value: nature; The prescription and the content of supporting base are joined in fermentation: soybean/tealeaves 68-80%, broken corn 8-18%, high-activity yeast 1.0-2.0%, glucose 2-7%, sucrose 3-9%, lime carbonate CaCO
30.8-1.6%, potassium primary phosphate KH
2PO
40.4-1.6%, sal epsom MgSO
40.3-0.9, zinc sulfate ZnSO
47H
2O0.12-0.25%, Sodium Selenite Na
2SeO
30.008-0.15%, and an amount of water.
2, the double fermentation process of a kind of Ganoderma lucidum mycelium powder as claimed in claim 1 or Ganoderma tea, it is characterized in that adopting tradition to carry out double fermentation with fungi glossy ganoderma and high-activity yeast, being in the raw material of major ingredient with high quality soybean or tealeaves, is to carry out oxygen enrichment fermentation 1-4 hour under 20 ℃-40 ℃ with high-activity yeast in room temperature; After pressure is to carry out 40-120 minute deactivation under the 0.08-0.15MPa, insert the ganoderma lucidum liquid bacterial classification again and carried out the aerobic pure-blood ferment 20-40 days, treat that mycelium covers with technologies such as back drying, pulverizing and forms Ganoderma lucidum mycelium powder or Ganoderma teas.
3, the complete processing of Ganoderma lucidum mycelium powder according to claim 2 or Ganoderma tea, comprise female cultivation, the preparation of liquid spawn and the preparation of fermention medium of planting of glossy ganoderma, it is characterized in that it is substratum that the glossy ganoderma mother culture media adopts improvement PDA, divide the threading test tube with prepared culture medium, after sterilizing, inoculate and cultivating, both can obtain that glossy ganoderma is female to be planted; The preparation method of liquid spawn: obtained the one grade fermemtation bacterial classification before this, take by weighing in proportion after the raw material of liquid fermentation medium removed defoamer, put into after the water heating for dissolving triangular flask packing then with 100-2000ml, the liquid amount of each triangular flask is 50-1000ml, in temperature is 121-127 ℃, pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, treat to begin inoculation after its cooling, carry out shaking culture again after waiting its kind ferfas silk to sprout, the rotating speed of vibrating machine remains on 100-200 rev/min, cultivates and can obtain the one grade fermemtation kind after 4-7 days; The preparation of second order fermentation bacterial classification: employing eats or medicinal fungi liquid spawn deep layer incubator carries out fermentation culture, put into the water dissolving back that is mixed after raw material in the liquid fermentation medium prescription taken by weighing in proportion and add incubator, in temperature is 121-127 ℃, pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, the one grade fermemtation bacterial classification is inserted in the cooling back, carry out aerated culture then, can obtain the second order fermentation bacterial classification in 6-8 days, the second order fermentation bacterial classification can be used for solid state fermentation.The preparation of fermention medium: in soybean: water=ratio took by weighing soybean in 1.0: 2.0, had measured water, earlier with Sodium Selenite Na
2SeO
3Soluble in water, again soybean bubble is opened and inhaled permeablely, drain away the water after 3-10 hour, stir after putting into other raw material again, add high-activity yeast at last.The pH value keeps nature, is to carry out oxygen enrichment fermentation 1-3 hour under 20 ℃-40 ℃ in room temperature; The solid fermentation container of then above-mentioned mixing raw material being packed into; In temperature is 131-127 ℃, and pressure is to sterilize 20-60 minute under the 0.11-0.15MPa condition, and the every bottle graft in natural step-down cooling back is gone into the ganoderma lucidum liquid bacterial classification; Be that 20 ℃-35 ℃, relative air humidity are condition bottom fermentation 20-40 days of 50-65% in temperature then, when mycelium covers with bottle, stop fermentation; It is under 60-80 ℃ in temperature that the back is finished in fermentation, carries out 10-18 hour oven dry processing, until its water content≤5%; Dried tunning is pulverized, and granularity is a standard to cross the 60-100 order.
4, Ganoderma lucidum mycelium powder according to claim 2 or Ganoderma tea is characterized in that adopting the two-stage liquid fermentation to obtain liquid spawn and inoculate.
5, Ganoderma lucidum mycelium powder according to claim 1 or Ganoderma tea is characterized in that the fungi glossy ganoderma can adopt red sesame, purple sesame, black sesame, blue or green sesame, Bai Zhi, rainbow conk or yellow sesame.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101624308B (en) * | 2009-08-14 | 2011-07-20 | 福建仙芝楼生物科技有限公司 | Ganoderma lucidum culture medium |
| CN101560118B (en) * | 2009-02-26 | 2012-07-04 | 浙江大学 | Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method |
| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN105613845A (en) * | 2015-12-23 | 2016-06-01 | 安徽省霍山县轩芝缘生物科技有限公司 | Detoxifying and beautifying ganoderma lucidum tea |
| CN105918513A (en) * | 2016-06-12 | 2016-09-07 | 于飞 | Lactarius deliciosus hypha fermenting tea and processing method thereof |
| CN106262807A (en) * | 2016-07-29 | 2017-01-04 | 江苏中祥高科技实业有限公司 | A kind of liquid fermentation height Ganoderma triterpenoids Ganoderma ferment and preparation technology thereof |
| CN107647009A (en) * | 2017-08-14 | 2018-02-02 | 湖南味菇坊生物科技股份有限公司 | Wave fine and soft ganoderma tea and preparation method |
| CN107711693A (en) * | 2017-10-27 | 2018-02-23 | 惠东县华昊生物科技有限公司 | A kind of ganoderma lucidum egg |
| CN115428848A (en) * | 2022-09-14 | 2022-12-06 | 韶关学院 | Ganoderma lucidum mycelium tea and preparation method thereof |
-
2006
- 2006-04-17 CN CN 200610035009 patent/CN1837354A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560118B (en) * | 2009-02-26 | 2012-07-04 | 浙江大学 | Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method |
| CN101624308B (en) * | 2009-08-14 | 2011-07-20 | 福建仙芝楼生物科技有限公司 | Ganoderma lucidum culture medium |
| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN103238464B (en) * | 2013-05-17 | 2014-10-08 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN105613845A (en) * | 2015-12-23 | 2016-06-01 | 安徽省霍山县轩芝缘生物科技有限公司 | Detoxifying and beautifying ganoderma lucidum tea |
| CN105918513A (en) * | 2016-06-12 | 2016-09-07 | 于飞 | Lactarius deliciosus hypha fermenting tea and processing method thereof |
| CN106262807A (en) * | 2016-07-29 | 2017-01-04 | 江苏中祥高科技实业有限公司 | A kind of liquid fermentation height Ganoderma triterpenoids Ganoderma ferment and preparation technology thereof |
| CN107647009A (en) * | 2017-08-14 | 2018-02-02 | 湖南味菇坊生物科技股份有限公司 | Wave fine and soft ganoderma tea and preparation method |
| CN107711693A (en) * | 2017-10-27 | 2018-02-23 | 惠东县华昊生物科技有限公司 | A kind of ganoderma lucidum egg |
| CN115428848A (en) * | 2022-09-14 | 2022-12-06 | 韶关学院 | Ganoderma lucidum mycelium tea and preparation method thereof |
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