CN105638232A - Domestic fungus liquid spawn culture method and medium thereof - Google Patents
Domestic fungus liquid spawn culture method and medium thereof Download PDFInfo
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Abstract
The invention provides a domestic fungus liquid spawn culture method and a medium thereof; the method comprises the following steps: a, providing micro-nano bubble water and medium raw materials of preset quantity, adding the medium raw materials into the micro-nano bubble water so as to obtain the bacterial strain medium, wherein the medium raw materials comprise bacterial strain culture needed carbon source, nitrogen source and inorganic salt; b, planting domestic fungus bacterial strain into the bacterial strain medium for the culturing process. The micro-nano bubble water is employed to replace tap water in the configuration of liquid spawn medium, and the novel medium is used for culturing the domestic fungus bacterial strain; compared with a conventional liquid spawn culture method, the novel method can obviously improve liquid spawn yield.
Description
Technical field
The invention belongs to biological technical field, particularly relate to a kind of edible fungus liquid culture growth medium and use this substratum to carry out the method for edible fungi liquid strain cultivation.
Background technology
Micro-nano liquid-gas interface technology is the new branch of science that new development in nearly more than ten years is got up, and can be produced in a liquid " micro-nano bubble " by this technology, thus form micro-nanobubble water. The micro-bubble of diameter between 50 microns to tens nanometer is produced when micro-nano bubble refers to that bubble occurs.
For common water body, micro-nanobubble water has great dispersity, about has ten million to receive micron bubble in the micro-nano water body of every milliliter. The oxygenation effect of micro-nano bubble in water body is quite high, and only a few hours just can make the water body dissolved oxygen (DO) in a big way improve rapidly. In addition, due to the vast number of nano bubble micro-in water body, in thermal motion process, micro-nano bubble and organism probability of collision are very big, be conducive to the oxygen carried by bubble or nutritive substance to penetrate in organism, organism absorption oxygen and nutritive substance are had certain activation. Micro-nano bubble is little owing to having bubble size, and the features such as specific surface area is big, adsorption efficiency height, lift velocity is slow in water, have important using value in fields such as flotation waste water treatment, water body oxygenation, bio-pharmaceuticals, precision chemical reactions.
Within 1948, mushroom has successfully been carried out Submerged liquid culturation by the U.S., has opened the mankind and has carried out the prelude that edible fungus liquid is cultivated and liquid spawn is studied. Liquid spawn is applied to the production of edible mushrooms, for edible mushrooms industry from the very complicated traditional mode of production, cycle length, cost height, with experience, spell labour, handicraft workshop formula to automatization, stdn, mass-producing upgrading be significant. Liquid spawn germination point is many, cell age is consistent, sprout after access planting material fast, field planting early, yield rate height, both having shortened the production cycle turn improves production efficiency.
For the cultivation of edible fungi liquid strain, except the nutritive substance of necessity, substratum also needs certain dissolved oxygen content, to provide the oxygen required for bacterial classification respiratory metabolism. Owing to tap water is generally having certain dissolved oxygen, with the substratum of tap water preparation also contains certain dissolved oxygen, it is provided that needed for the metabolism of bacterial classification, therefore traditional liquid spawn culture medium can't increase the oxygen level in substratum specially. But, the dissolved oxygen in tap water is limited after all, can only meet predetermined amount bacterial classification metabolism needed for, can not fully meet the demand of whole bacterial classification.
Pleurotus eryngii is that exploitation cultivation successfully integrates edible, medicinal, the Rare edible fungus new variety of dietotherapy.Pleurotus eryngii is nutritious, the mineral substance such as rich in proteins, carbohydrate, VITAMIN and calcium, magnesium, copper, zinc, there is reducing blood-fat, decreasing cholesterol, promotion gastro-intestinal digestion, enhancing body immunological competence, prevent the effects such as cardiovascular diseases, pole is liked by people, has higher cultivating value and commercial value. Pleurotus eryngii liquid spawn is the focus that China's Pleurotus eryngii industrial is produced in recent years. At present, the domestic research and development major part to Pleurotus eryngii liquid spawn technology concentrates in the optimization of Pleurotus eryngii liquid culture based formulas and fermentation technique. The culture medium prescription of Pleurotus eryngii liquid culture is simple as far as possible, is easy to standardized production to control the quality of liquid spawn.
Up to the present, the impact of edible fungi growth is not yet had relevant report by micro-nanobubble water.
Summary of the invention
It is an object of the present invention to provide a kind of edible fungi liquid strain cultural method, it uses micro-nanobubble water to replace the substratum of tap water obtaining liq bacterial classification, and use this substratum to be cultivated by the bacterial classification of edible mushrooms, the method, compared with traditional strain cultivation method, can significantly improve the output of liquid spawn.
Another object of the present invention is to provide a kind of edible fungi liquid strain cultural method, this method increases the dissolved oxygen content in substratum, and the oxygen being dissolved in substratum in the medium the residence time long.
Another object of the present invention is to provide a kind of edible fungi liquid strain cultural method, and the method can be applied to the substratum of different ingredients, for improving the output of liquid spawn.
Another object of the present invention is to provide a kind of edible fungi liquid strain cultural method, and the method does not need complicated step or extra additive, so that it may to improve the output of liquid spawn.
Another object of the present invention is to provide a kind of edible fungi liquid strain cultural method, and the method is used for wood-decay fungi if Pleurotus eryngii, needle mushroom etc. or straw rotting fungus are such as the cultivation of the liquid spawn such as Twospore Mushroom, straw mushroom, can improve the output of liquid spawn.
Another object of the present invention is to provide a kind of edible fungi liquid strain cultural method, and the method is simple, cost is low, energy-saving efficiency height, need not change the formula of substratum, so that it may so that the output increased of liquid spawn.
Another object of the present invention is to provide a kind of edible fungus liquid culture growth medium, this substratum uses micro-nanobubble water to replace the substratum of tap water obtaining liq bacterial classification, use this substratum that edible fungus species is carried out fermentation culture, the output of liquid spawn can be significantly improved, and when reaching the bacterial classification output wanted, it is possible to obviously shorten incubation time.
Another object of the present invention is to provide a kind of edible fungus liquid culture growth medium, and the dissolved oxygen content in this substratum is higher than the dissolved oxygen content of traditional substratum.
For reaching above object, the present invention provides the cultural method of a kind of edible fungi liquid strain, and it comprises the following steps:
A) by dissolving culture medium raw material in micro-nanobubble water, bacterium culture medium is obtained, described substratum
Raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt; With
B) bacterial classification of edible mushrooms is accessed described bacterium culture medium, cultivate.
Further, before step a, also comprise a step c: use micro-nano bubble aerating apparatus to produce micro-nano bubble in water body, form micro-nanobubble water.
Further, before step b, a steps d is also comprised: described substratum is carried out sterilizing.
Further, in step a, the micro-nano bubble in described micro-nanobubble water is of a size of 50-150nm.
Further, in step a and step c, the gas of the micro-nano bubble in described micro-nanobubble water is air.
Further, the dissolved oxygen content of the described micro-nanobubble water in step a is higher than the dissolved oxygen content of ordinary tap water.
Further, the water body used in step c is the water of more than three class standards.
Further, the culture medium raw material in step a comprises: white sugar 10-30g/L, bean cake powder 2-5g/L, MgSO40.2-1.0g/L��K2HPO40.2-1.2g/L��
Further, the edible mushrooms in step (b) is selected from wood-decay fungi and straw rotting fungus, the one that described wood-decay fungi is selected from Pleurotus eryngii and needle mushroom, the one that described straw rotting fungus is selected from Twospore Mushroom and straw mushroom.
Further, in described bacterium culture medium, the numerical value of dissolved oxygen is 10-15mg/L.
The present invention also provides the distortion of the cultural method of above-described a kind of edible fungi liquid strain, and it comprises the following steps:
E () provides the water of predetermined amount and the culture medium raw material of predetermined amount, added by described culture medium raw material in described water, forms mixing solutions, and described culture medium raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt;
F () produces micro-nano bubble by micro-nano bubble aerating apparatus in described mixing solutions, obtain bacterium culture medium; With
G the bacterial classification of edible mushrooms is accessed described bacterium culture medium by (), cultivate.
Further, before step (g), a step (d) is also comprised: described substratum is carried out sterilizing.
The present invention also provides a kind of edible fungus liquid culture growth medium, its component comprises micro-nanobubble water of predetermined amount and is dissolved in the culture medium raw material of the predetermined amount of described micro-nanobubble water, and described culture medium raw material comprises cultivates the necessary carbon source of edible fungus species, nitrogenous source and inorganic salt.
Edible fungus liquid culture growth medium provided by the invention is obtained by the technique comprised the following steps:
Steps A: produce micro-nano bubble in water by micro-nano bubble aerating apparatus, form micro-nanobubble water; With
Step B: the culture medium raw material adding predetermined amount in described micro-nanobubble water, forms substratum, and described culture medium raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt.
Edible fungus liquid culture growth medium provided by the invention can also be obtained by the technique comprised the following steps:
Step C: Xiang Shuizhong adds the culture medium raw material of predetermined amount, forms mixing solutions, and described culture medium raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt; With
Step D: produce micro-nano bubble in described mixing solutions by micro-nano bubble aerating apparatus, obtain bacterium culture medium.
Accompanying drawing explanation
Fig. 1 is the interfacial state schematic diagram of micro-nano bubble, shows " two film " structure of micro-nano bubble and water termination.
Embodiment
Those skilled in the art below describe for disclosing the present invention so that can realize the present invention. Preferred embodiment in below describing only as an example, it may occur to persons skilled in the art that other apparent modification. The ultimate principle of the present invention defined in the following description can be applied to other embodiments, deformation program, improvement scheme, equivalent and not deviate from the other technologies scheme of the spirit and scope of the present invention.
The present invention provides the cultural method of a kind of edible fungi liquid strain, comprises the following steps:
A () provides micro-nanobubble water of predetermined amount and the culture medium raw material of predetermined amount, described culture medium raw material is added in described micro-nanobubble water, preparation obtains bacterium culture medium, and described culture medium raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt.That is, by adding a culture medium raw material in a micro-nanobubble water, form a bacterium culture medium.
B the bacterial classification of edible mushrooms is accessed described bacterium culture medium by (), cultivate.
Preferably, can increase by a step before step (a): (c) uses micro-nano bubble aerating apparatus to produce micro-nano bubble in water body, forms micro-nanobubble water.
The glassware for drinking water used in step (c) has certain purity, it may be preferred that the water used in step c is the water of more than three class standards.
In step (a), the micro-nano bubble in described micro-nanobubble water is of a size of 10 nanometers-50 microns. By selecting suitable micro-nano bubble aerating apparatus, it is possible to make the size of micro-nano bubble preferably reach 50-150 nanometer.
Preferably, in step (a), the gas of described micro-nano bubble is air, that is, by micro-nano bubble aerating apparatus, air is injected water and prepares micro-nanobubble water.
Preferably, dissolved oxygen amount (DO) value of the described nanobubble water in step (a) is higher than the dissolved oxygen content of ordinary tap water. By producing micro-nano bubble in ordinary tap water, form described micro-nanobubble water, owing to micro-nano bubble has the effect increasing dissolved oxygen content, so the DO content of described micro-nanobubble water is higher than the DO content of the ordinary tap water not containing micro-nano bubble. It should be noted that experimentally result, the scope preferred 10-15mg/L of the DO value in the edible fungus species substratum of the present invention under culture temperature. That is, edible fungus species substratum is suitable in this preferred OK range, and the effect not only ensureing to increase dissolved oxygen content can prevent again dissolved oxygen too much, suppresses the growth of edible mushrooms.
In step (a), the carbon source in described culture medium raw material mainly selects one or more from following material: sugar and sugared derivative, organic acid and alcohols material etc., such as glucose, sucrose, Semen Maydis powder, wheat bran, rice bran, sawmilling, cotton seed hulls. In described culture medium raw material, nitrogenous source mainly selects one or more from following material: the inorganic nitrogens such as the organonitrogens such as protein, amino acid, urea and ammonia, ammonium salt, nitrate, such as corn, bean cake powder. Described inorganic salt mainly select one or more from the inorganic salt comprising the elements such as phosphorus, Calcium Magnesium Sulphur, potassium, such as potassium primary phosphate, magnesium sulfate. Described culture medium raw material selects different kinds and consumption according to different edible fungus species.
Preferably, the bacterial classification in step (b) is Pleurotus eryngii bacterial classification.
Preferably, the culture medium raw material in step (a) and ratio in the medium thereof are: white sugar 10-30g/L, bean cake powder 2-5g/L, MgSO40.2-1.0g/L��K2HPO40.2-1.2g/L��
Preferably, step (b) constant temperature concussion at 15-30 DEG C is cultivated 3-10 days. Preferably, step b constant temperature concussion at 25 DEG C is cultivated 7 days.
Micro-nanobubble water in step (c) can also use other devices of similar micro-nano bubble aerating apparatus to obtain.
If the water body used in step (c) does not reach three class standard water, first the water body used can be purified, the method of purification can adopt various purifying methods conventional at present, micro-nanobubble water can also be utilized, remove the organism in water, as lower in solubleness such as agricultural chemicals and organism that toxicity ratio is bigger. Its method injects micro-nano bubble in stand-by water, and through after a while, the water surface has floated one layer of film, is removed by this layer of film and just can remove quite a lot of organism.
Preferably, a step (d) can be increased before step (b): described substratum is carried out sterilizing.
In step (d), the method for sterilizing can adopt the conventional various methods that substratum carries out sterilizing, such as high-temperature sterilization. According to practical situation, suitable sterilising method should be selected.
Following step is the distortion of the cultural method of above-described a kind of edible fungi liquid strain, comprises the following steps:
E () provides the water of predetermined amount and the culture medium raw material of predetermined amount, added by described culture medium raw material in described water, forms mixing solutions, and described culture medium raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt. In other words, by adding a culture medium raw material in a water body, form mixing solutions.
F () produces micro-nano bubble by micro-nano bubble aerating apparatus in described mixing solutions, obtain bacterium culture medium.
G the bacterial classification of edible mushrooms is accessed described bacterium culture medium by (), cultivate.
That is the injection of micro-nano bubble both can inject water with described culture medium raw material before water mixes, it is also possible to injects mixed solution after water mixes with described culture medium raw material. Namely water can be obtained micro-nanobubble water by the process of micro-nano bubble aerating apparatus, then culture medium raw material be added in described micro-nanobubble water, to obtain described bacterium culture medium; Can also be that described culture medium raw material is first dissolved completely in water, to obtain the substratum work in-process of liquid, then make described substratum work in-process produce micro-nano bubble by the process of micro-nano bubble aerating apparatus, thus obtain having the described bacterium culture medium of micro-nano bubble.
Step (e) uses the water with certain purity, it may be preferred that the water body of use is the water of more than three class standards.
In step (e), the carbon source in described culture medium raw material mainly selects one or more from following material: sugar and sugared derivative, organic acid and alcohols material etc., such as glucose, sucrose, Semen Maydis powder, wheat bran, rice bran, sawmilling, cotton seed hulls. In described culture medium raw material, nitrogenous source mainly selects one or more from following material: the inorganic nitrogens such as the organonitrogens such as protein, amino acid, urea and ammonia, ammonium salt, nitrate, such as corn, bean cake powder. Described inorganic salt mainly select one or more from the inorganic salt comprising the elements such as phosphorus, Calcium Magnesium Sulphur, potassium, such as potassium primary phosphate, magnesium sulfate. Described culture medium raw material selects different kinds and consumption according to different edible fungus species.
Preferably, culture medium raw material and the ratio thereof in step (e) is: white sugar 10-30g/L, bean cake powder 2-5g/L, MgSO40.2-1.0g/L��K2HPO40.2-1.2g/L��
In step (f), it is possible to use other devices of similar micro-nano bubble aerating apparatus produce micro-nano bubble in described mixing solutions.
In step (f), the micro-nano bubble in described mixing solutions is of a size of 10 nanometers-50 microns. Preferably, the micro-nano bubble produced in described mixing solutions is of a size of 50-150nm.
Preferably, in step (f), the gas of described micro-nano bubble is air.
In described mixing solutions, inject micro-nano bubble by micro-nano bubble aerating apparatus, the DO value of the described mixing solutions obtained can be made to increase. If the water body used in step (e) does not reach three class standard water, first the water body used can be purified, the method of purification can adopt various purifying methods conventional at present, micro-nanobubble water can also be utilized, remove the organism in water, as lower in solubleness such as agricultural chemicals and organism that toxicity ratio is bigger.Its method injects micro-nano bubble in stand-by water, and through after a while, the water surface has floated one layer of film, is removed by this layer of film and just can remove quite a lot of organism.
Preferably, a step (d) can be increased before step (g): described substratum is carried out sterilizing.
In step (d), the method for sterilizing can adopt the conventional various methods that substratum carries out sterilizing, such as high-temperature sterilization. According to practical situation, suitable sterilising method should be selected.
The present invention also provides the substratum of a kind of edible fungi liquid strain, and its component comprises micro-nanobubble water of predetermined amount and the culture medium raw material of predetermined amount, and described culture medium raw material comprises cultivates the necessary carbon source of edible fungus species, nitrogenous source and inorganic salt.
Wherein, described micro-nanobubble water by micro-nano bubble aerating apparatus produce in water body micro-nano bubble obtain. Micro-nano bubble in described micro-nanobubble water is of a size of 10 nanometers-50 microns. Preferably, micro-nano bubble is of a size of 50-150nm. The gas of described micro-nano bubble is air. The DO value of described micro-nanobubble water is higher than the DO value injecting the water body before micro-nano bubble.
Edible fungus liquid culture growth medium provided by the invention is obtained by the technique comprised the following steps:
(A) in water, produce micro-nano bubble by micro-nano bubble aerating apparatus, form micro-nanobubble water.
(B) adding the culture medium raw material of predetermined amount in described micro-nanobubble water, form substratum, described culture medium raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt.
Preferably, the water used in step (A) is the water of more than three class standards.
As distortion, the edible fungus liquid culture growth medium of the present invention can also be obtained by the technique comprised the following steps:
(C) Xiang Shuizhong adds the culture medium raw material of predetermined amount, forms mixing solutions, and described culture medium raw material includes cultivates the necessary carbon source of bacterial classification, nitrogenous source and inorganic salt.
(D) in described mixing solutions, produce micro-nano bubble by micro-nano bubble aerating apparatus, obtain bacterium culture medium.
Preferably, the water used in step (C) is the water of more than three class standards.
The ratio of described micro-nanobubble water and described culture medium raw material, according to the practical situation used, with reference to the ratio-dependent of ortho-water and culture medium raw material.
The ultimate principle of the present invention is as follows:
1) air with the micron even size dispersion of nanometer size in aqueous phase, define micro-nano liquid-gas interface system, this kind of system has many features: the oxygen content in water can be very high, water also exists the oxygen that molecule dissolves form and micro-nano rice bubble shape two kinds of forms, thus in water body, oxygen significantly increases, and can exceed the several times of solubleness.
2) as shown in Figure 1, on micro-nano liquid-gas interface, oxygen more easily trends towards interfacial layer than nitrogen, because the electronegativity of oxygen is more suitable for forming hydrogen bond with water, makes the micro-nano rice bubble formation " two membrane structure " in water body, thus has good stability.
3) micro-nano bubble has great interface, when interface changes (such as coalescence), has very big energy variation, thus brings very big activity, some biologies are had activation.
4) micro-nano bubble has great dispersity, micro-nano rice water body about has in every 1 milliliter ten million micro-nano bubble, in thermal motion process very big with organism probability of collision, it is easier to penetrate in organism, absorbed by many cells aerobe body, and the materials such as the close oxygen albumen existed in organism absorb oxygen, and promote the absorption of nitre nitrogen, and then the energy needed for aerobic repiration of the organism such as carbohydrate, lipid, protein in organism is provided, to help body growth.
5) area of micro-nano rice bubble interface is very big, organism in water body is more easily adsorbed on liquid-vapo(u)r interface, the organism on the water surface is made to reduce like this, increase water_air exchange, the effect improving water quality can be reached, when processes such as micro-nano bubble generation coalescences, stronger oxygenizement is had to occur, again can the water quality of purifying water body further.
According to above principle, it may be determined that the bacterium culture medium that the tap water that the present invention uses micro-nanobubble water to replace tradition to use is prepared is conducive to the growth of bacterial classification, it is possible to bring useful effect.
The edible fungi liquid strain cultural method of the present invention is applicable to the cultivation of various edible fungi, is also applicable to the substratum being made up of the culture medium raw material of different components, different ratios. Described edible mushrooms can be that wood-decay fungi is if Pleurotus eryngii, needle mushroom etc. or straw rotting fungus are such as Twospore Mushroom, straw mushroom etc. In following examples, for the edible fungus species of the present invention as Pleurotus eryngii bacterial classification is specifically described.
In the examples below, Pleurotus eryngii bacterial classification is selected to carry out concrete experiment, so that the useful effect of the present invention to be described. The experimental technique of unreceipted concrete condition in the following example, usual conveniently condition, condition or the condition of production firm's recommendation as described in " molecular cloning: laboratory manual " (New York: cold whole Hong Kong laboratory Press of the U.S., 1989) carry out.
Embodiment 1, DO value carry out Pleurotus eryngii strain cultivation for the micro-nanobubble water of 10mg/L
The DO value of offer predetermined amount is micro-nanobubble water of 10mg/L, and the tap water of predetermined amount, and its DO value is 6mg/L. Thering is provided two parts of culture medium raw materials, it comprises respectively: white sugar 20g/L, bean cake powder 3g/L, MgSO40.6g/L��K2HPO40.6g/L, uses micro-nanobubble water and tap water to dissolve fixed appearance respectively, forms two parts of bacterium culture mediums.
The Pleurotus eryngii bacterial classification of equivalent is accessed respectively in two parts of bacterium culture mediums. At 25 DEG C, constant-temperature shaking culture is after 7 days, and the nutrient solution filter paper filtering getting 150ml from above-mentioned two parts of nutrient solutions respectively goes out bacterium ball, and washing is for several times, then putting into loft drier, at 105 DEG C, dry 2h, is dried, weigh after cooling, to measure the biomass of Pleurotus eryngii mycelium pellet. Result is as shown in table 1.
Table 1, DO value are that the micro-nanobubble water of 10mg/L is on the impact of Pleurotus eryngii liquid spawn biomass
| DO value (mg/L) | Hypha biomass (g/L) | |
| Tap water | 6 | 3.67 |
| Micro-nanobubble water | 10 | 4.50 |
Embodiment 2, DO value carry out Pleurotus eryngii strain cultivation for the micro-nanobubble water of 15mg/L
The DO value of offer predetermined amount is micro-nanobubble water of 15mg/L, and the tap water of predetermined amount, and its DO value is 6mg/L. Thering is provided two parts of culture medium raw materials, it comprises respectively: white sugar 20g/L, bean cake powder 3g/L, MgSO40.6g/L��K2HPO40.6g/L, uses micro-nanobubble water and tap water to dissolve fixed appearance respectively, forms two parts of bacterium culture mediums.
The Pleurotus eryngii bacterial classification of equivalent is accessed respectively in two parts of bacterium culture mediums. At 25 DEG C, constant-temperature shaking culture is after 7 days, and the nutrient solution filter paper filtering getting 150ml from above-mentioned two parts of nutrient solutions respectively goes out bacterium ball, and washing is for several times, then putting into loft drier, at 105 DEG C, dry 2h, is dried, weigh after cooling, to measure the biomass of Pleurotus eryngii mycelium pellet. Result is as shown in table 2.
Table 2, DO value are that the micro-nanobubble water of 15mg/L is on the impact of Pleurotus eryngii liquid spawn biomass
| DO value (mg/L) | Hypha biomass (g/L) | |
| Tap water | 6 | 3.67 |
| Micro-nanobubble water | 15 | 4.67 |
According to above two embodiments it may be seen that the substratum using micro-nanobubble water to prepare more is conducive to the growth of Pleurotus eryngii bacterial classification than the substratum that tap water is prepared, it is possible to significantly improve the output of Pleurotus eryngii mycelia, also namely improve the output of Pleurotus eryngii liquid spawn.
Embodiment 3, DO value are the Pleurotus eryngii strain cultivation that the micro-nanobubble water of 15mg/L carries out different time
The DO value of offer predetermined amount is micro-nanobubble water of 15mg/L, and the tap water of predetermined amount, and its DO value is 6mg/L.Thering is provided two parts of culture medium raw materials, it comprises respectively: white sugar 20g/L, bean cake powder 3g/L, MgSO40.6g/L��K2HPO40.6g/L, uses micro-nanobubble water and tap water to dissolve fixed appearance respectively, forms two parts of bacterium culture mediums.
The Pleurotus eryngii bacterial classification of equivalent is accessed respectively in two parts of bacterium culture mediums. At 25 DEG C, constant-temperature shaking culture is after 6 days and 7 days respectively, and the nutrient solution filter paper filtering getting 150ml from above-mentioned two parts of nutrient solutions respectively goes out bacterium ball, and washing is for several times, then putting into loft drier, at 105 DEG C, dry 2h, is dried, weigh after cooling, to measure the biomass of Pleurotus eryngii mycelium pellet. Result is as shown in table 3.
Table 3, DO value are that the micro-nanobubble water of 15mg/L is on the impact of Pleurotus eryngii liquid spawn biomass
As can be seen from the above table, adopt the liquid spawn culture medium of micro-nanobubble water than the liquid spawn culture medium adopting tap water, it is possible to roughly to put forward the hypha biomass reaching substantially identical the day before yesterday. That is, because have employed the liquid spawn culture medium of micro-nanobubble water, when reaching the hypha biomass wanted, the required time shortens greatly, like this, when large-scale production and application, it is possible to improve production benefit significantly.
Embodiment 4, DO value are the fermentation culture that the micro-nanobubble water of 15mg/L carries out Pleurotus eryngii liquid spawn
Preparing two parts of fermention mediums, described fermention medium is identical with the ratio of the nutritional medium raw material of the liquid spawn culture medium in described embodiment 2, namely can be white sugar 10-30g/L, bean cake powder 2-5g/L, MgSO40.2-1.0g/L��K2HPO40.2-1.2g/L. And it should be noted that the water in two parts of described fermention mediums is micro-nanobubble water and tap water respectively.
Above-mentioned fermention medium is poured in two fermentor tanks respectively, after sterilizing, after its temperature cools, as placed a water shower device on tank body with rapid cooling tank body, can connect liquid matrix bacterial classification when tank body temperature degree is cooled to below 25 DEG C. In this preferred embodiment, then by embodiment 2 cultivate 7 days liquid spawn respectively with 1-2 �� and identical inoculum size accesses in described fermentor tank, fermentor cultivation temperature is 21-25 DEG C, leads to into sterile air, and air pressure is 0.5Mpa. Cultivate and measure hypha biomass after 7 days, the results are shown in following table 4.
Table 4, DO value are the impact that Pleurotus eryngii liquid fungus seed is cultivated by the micro-nanobubble water of 15mg/L
| DO value (mg/L) | Hypha biomass (g/L) | |
| Tap water | 6 | 4.24 |
| Micro-nanobubble water | 15 | 5.21 |
It may be seen that work as in described fermention medium from experiment above, when utilizing micro-nanobubble water to dissolve culture medium raw material, when culture condition is the same, the hypha biomass finally obtained improves significantly. It should be noted that when traditional liquid fungus seed is cultivated, all need to lead to into sterile air. And the present invention uses micro-nanobubble water to increase dissolved oxygen, and improve water quality, thus when identical incubation time, hypha biomass significantly improves, and when reaching identical hypha biomass, required incubation time is short. And when the situation that hypha biomass requirement is lower, it is possible to do not need to lead to into air when fermentation culture, so more significantly decrease production cost, it is to increase production benefit.
It should be understood by those skilled in the art that, the embodiments of the invention shown in foregoing description and accompanying drawing only do not limit the present invention. The object of the present invention is complete and effectively realizes. The function of the present invention and structural principle are shown in an embodiment and are illustrated, are not deviating under described principle, and embodiments of the present invention can have any distortion or amendment.
Claims (10)
1. the cultural method of an edible fungi liquid strain, it is characterised in that, comprise the following steps:
A (), by dissolving culture medium raw material in micro-nanobubble water, forms a bacterium culture medium; With
B the bacterial classification of edible mushrooms is accessed described bacterium culture medium and cultivates by ().
2. the cultural method of edible fungi liquid strain as claimed in claim 1, it is characterized in that, a step (c) was also comprised: use micro-nano bubble aerating apparatus to produce micro-nano bubble in water, form micro-nanobubble water before step (a).
3. the cultural method of edible fungi liquid strain as claimed in claim 1, it is characterised in that, the gas of the micro-nano bubble in described micro-nanobubble water is air, and the micro-nano bubble in described micro-nanobubble water is of a size of 50-150nm.
4. the cultural method of edible fungi liquid strain as claimed in claim 1, it is characterised in that, described edible mushrooms is selected from wood-decay fungi and straw rotting fungus, the one that described wood-decay fungi is selected from Pleurotus eryngii and needle mushroom, the one that described straw rotting fungus is selected from Twospore Mushroom and straw mushroom.
5. the cultural method of edible fungi liquid strain as claimed in claim 1, it is characterised in that, the culture medium raw material in step (a) comprises: white sugar 10-30g/L, bean cake powder 2-5g/L, MgSO40.2-1.0g/L and K2HPO40.2-1.2g/L��
6. the cultural method of edible fungi liquid strain as claimed in claim 1, it is characterised in that, in described bacterium culture medium, the numerical value of dissolved oxygen is 10-15mg/L.
7. the cultural method of edible fungi liquid strain as claimed in claim 1, it is characterized in that, in step (a), water is obtained micro-nanobubble water by the process of micro-nano bubble aerating apparatus, then described culture medium raw material is added in described micro-nanobubble water, to obtain described bacterium culture medium; Or, described culture medium raw material is first dissolved completely in water, to obtain the substratum work in-process of liquid, then make described substratum work in-process produce micro-nano bubble by the process of micro-nano bubble aerating apparatus, thus obtain having the described bacterium culture medium of micro-nano bubble.
8. an edible fungus liquid culture growth medium, it is characterized in that, comprise: micro-nanobubble water of predetermined amount and the culture medium raw material of predetermined amount being dissolved in described micro-nanobubble water, described culture medium raw material comprises cultivates the necessary carbon source of edible fungus species, nitrogenous source and inorganic salt.
9. edible fungus liquid culture growth medium as claimed in claim 8, it is characterised in that, the gas of the micro-nano bubble in described micro-nanobubble water is air, and the micro-nano bubble in described micro-nanobubble water is of a size of 50-150nm.
10. edible fungus liquid culture growth medium as claimed in claim 8 or 9, it is characterised in that, described culture medium raw material comprises: white sugar 10-30g/L, bean cake powder 2-5g/L, MgSO40.2-1.0g/L and K2HPO40.2-1.2g/L��
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