CN103314770A

CN103314770A - Fluid culture preparing method for bamboo fungus

Info

Publication number: CN103314770A
Application number: CN2012100798900A
Authority: CN
Inventors: 黄文培; 游玲; 王超
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-03-25
Filing date: 2012-03-25
Publication date: 2013-09-25

Abstract

The invention provides a culture preparing method for bamboo fungus, which includes the following steps: adopting a kernel solid medium for culture activation under proper culture condition, adopting soybean fluid medium to cultivate activated cultures, and finally obtaining a bamboo fungus fluid culture with uniform and dense mycelium pellets with proper sizes, then inoculating the culture to a bamboo slag solid medium for cultivation, and obtaining the cultivated culture. According to the method, time in culture preparation is shortened, the culture preparation efficiency is improved, and the cultivated culture is suitable for traditional cultivation patterns.

Description

A kind of liquid spawn preparation method of Cultivation of Dictyophora

Technical field

The present invention relates to the liquid producing method for seed of Cultivation of Dictyophora, main with the Cultivation of Dictyophora bacterial classification inoculation in the soybean liquid nutrient medium of certain prescription, utilize the shaking table oscillation action to form a large amount of tiny mycelium pellets, shorten the production of hybrid seeds time, improve seed production efficiency.

Background technology

Cultivation of Dictyophora has another name called bamboo ginseng, grenadine bacterium, belongs to Basidiomycota, Basidiomycetes, and Phallales, Phallaceae is a kind of edible and medicinal fungi of preciousness.From eighties in 20th century first since the domestication, after for many years production practices, selected and be fit to tame high yield and high quality bacterial strain, cultivate in a large number in provinces and cities such as Fujian, Sichuan, at present main seed production way is for utilizing bamboo slag solid culture medium to prepare one-level and secondary cultivated species, prepare 50 tons of cultivated speciess and need 4-5 labour time spent 8-12 month, and through repeatedly long-time expansion cultivation and switching, easily cause the degeneration of mycelia, the fruit body cultivation underproduction.

Summary of the invention

The objective of the invention is to replace the conventional solid seed production way with the mode of the liquid production of hybrid seeds, to shorten the production of hybrid seeds time and to reduce labour intensity.

Wheat culture medium prescription: wheat 95%, weed tree sawdust 5%.Fresh wheat soaks 12 h, washes 3 times, and boiling water boiling adds water cooling to without the white heart, adds 5% weed tree sawdust and mixes thoroughly, 121 ℃ of sterilization 30 min.

The prescription of soybean liquid nutrient medium: soybean extract 200 ml, soluble starch 20 g, peptone 10 g, dusty yeast 10 g, potassium dihydrogen phosphate 3 g, magnesium sulfate 1 g, calcium carbonate 1 g, vitamin b1 0.01 g distilled water 800 ml, pH 6.0.

The wherein preparation of soybean extract: 20 g fresh soyabeans are pulverized, boiling water boiling 10 min, three layers of filtered through gauze are settled to 200ml and namely get soybean extract.

The object of the present invention is achieved like this: inoculate first 1 test tube slant bacterial classification (the mycelium weight in wet base is 0.5-1 g approximately) in above-mentioned wheat medium, cultivate 20-30 d for 25 ℃, until grow dense mycelia, add again the above-mentioned soya broth (liquid amount 40%) of 20 times of quality, 25 ℃, 150 r/min cultivate 7 d, form suitable size and the intensive mycelium pellet of homogeneous, i.e. liquid spawn.

Effect of the present invention is: adopt traditional producing method for seed, the experienced operator inoculates 800-1000 bottle meter every day, adopts the liquid seed production way, the experienced operator can inoculate the 6000-7000 bottle every day, efficient improves greatly, makes 50 tons of cultivated speciess, foreshortens to 3-4 month from original 8-12 month.Under the prerequisite of shortening time, assurance strain quality, improved seed production efficiency, produce for the Cultivation of Dictyophora batch production and lay a good foundation.

Embodiment

Embodiment 1:

Get wheat 950 g flushing 2-3 time of going mouldy of commercially available nothing, clear water soak at room temperature 12 h, filtration and washing 3 times, add poach to wheat without the white heart, filter, add cold water and be cooled to room temperature, filter, the wet wheat after the filtration adds 50 g weed tree sawdusts and mixes thoroughly, the pH nature, equivalent is distributed into 4 500 ml triangular flasks, and 121 ℃ of sterilization 30 min are cooled to room temperature, every bottle graft enters 1 test tube slant and preserves bacterial classification (the mycelia weight in wet base is 0.5-1 g approximately), the jog triangular flask is evenly distributed between the wheat bacterial classification, and 25 ℃ of constant temperature culture are until mycelia is covered with triangular flask.

Get 250 ml triangular flasks, 121 ℃ of empty bottle sterilization 20min, above-mentioned kernel culture 5 g are transferred in this triangular flask, 25 ℃ are cultured to and grow dense aerial hyphae, add 100 ml at 121 ℃ of sterilization 20min again, and are cooled to the soybean liquid nutrient medium of room temperature, 25 ℃ again, 150 r/min shaken cultivation, 7 d, inoculate aseptic bamboo slag medium by 2% mass ratio immediately, 25 ℃ are cultured to full bottle, can be directly as cultivated species.Adopt traditional sowing, management method, use the dictyophora phalloidea yield and quality of producing behind this cultivated species slightly to be better than the Cultivation of Dictyophora that uses the traditional cultivation kind to produce.

Claims

1. the soybean liquid culture based formulas that is used for the Cultivation of Dictyophora bacterial classification: soybean extract 200g, soluble starch 20 g, peptone 10 g, dusty yeast 10 g, potassium dihydrogen phosphate 3g, magnesium sulfate 1 g, calcium carbonate 1g, vitamin b10.01g distilled water 800ml, pH value 6.

2. above-mentioned two kinds of medium are occupation modes: inoculate 1 test tube slant bacterial classification (the mycelium weight in wet base is 0.5-1 g approximately) in the wheat medium, cultivate 20-30 d for 25 ℃, until grow dense mycelia, add again the aforesaid liquid medium (liquid amount 40%) of 20 times of quality, 25 ℃, 150 r/min cultivate 7 d.