CN107142216A - A kind of stable method for cultivating Phellinus strain of scale - Google Patents
A kind of stable method for cultivating Phellinus strain of scale Download PDFInfo
- Publication number
- CN107142216A CN107142216A CN201710455750.1A CN201710455750A CN107142216A CN 107142216 A CN107142216 A CN 107142216A CN 201710455750 A CN201710455750 A CN 201710455750A CN 107142216 A CN107142216 A CN 107142216A
- Authority
- CN
- China
- Prior art keywords
- phellinus
- temperature
- strain
- phellinus strain
- cultivates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to Phellinus breeding strain technical field, there is provided a kind of stable method for cultivating Phellinus strain of scale.This method is accessed Phellinus strain on zeolite powder and diatomaceous complex in desinfection chamber by the way that by high-temperature sterilization after zeolite powder and composite diatomite, certain temperature is quickly cooled to using liquid nitrogen.Then water, mulberry tree wood sawdust, carbon source, nitrogen source, phosphate fertilizer, potash fertilizer, magnesium sulfate and agar powder prepared media are used, and uses high pressure steam sterilization.Load waffle slab after the complex for being vaccinated with Phellinus bacterium is mixed in proportion with Nutrient medium again, with Plastic Package, be put into culturing room, control the temperature and relative humidity of culturing room, and keep dark state, uniform mycelia is can obtain after culture certain time.Compared with conventional method, the method for the invention cultivates Phellinus strain, and survival rate is high, and large-scale cultivation can be achieved in steady quality.
Description
Technical field
The present invention relates to Phellinus breeding strain technical field, more particularly, to a kind of stable cultivation Phellinus strain of scale
Method.
Background technology
Phellinus is a kind of precious medicinal fungi, is described as " forest gold ".Its fructification is used as medicine, and can control metrorrhagia, blood strangury
With the gynecological disease such as amenorrhoea, its anticancer, antibacterial, anti-oxidant and anti-fibrotic effects are also very significantly, therefore turn into research in recent years
Focus.Japan and research of the South Korea to Phellinus are more and more deep.Because wild Phellinus is incomparable to the requirement of growing environment condition tight
It is severe, and the growth cycle of 20 ~ 30 years is needed, therefore it is extremely rare, supply falls short of demand, and price is very expensive.Thus, artificial culture can be made
There is very big necessity for the Phellinus of pharmaceutical raw material.Separation and cultivation to Phellinus strain are the first of artificial culture Phellinus
Step, it appears particularly important.The research of China in this respect is just in the starting stage, and some existing researchers have been prepared containing various
The Phellinus bacterium culture medium of nutritional ingredient, but obtained strain survival rate is not high, and quality is not sufficiently stable.
Gao etc. has invented a kind of breeding method of Phellinus strain, and the culture medium quality proportioning composition of preparation is：Willow or willow
Tree or mulberry tree wood chip 75~85%, wheat bran 10~15%, rice bran 5~8%, gypsum 1~2%, potassium dihydrogen phosphate 0.2~0.5%,
Magnesium sulfate 0.1~0.3%, the Phellinus strain bacterium germination cultivated is fast, and surface mycelia is non-aging, and 30~35d of culture can be whole
Hair is full, and the Phellinus individual of output is big, simple to operate, and a large amount of artificial cultivations can be achieved.Wang etc. first prepares culture medium, then in nothing
Under collarium border, Phellinus strain block is taken with inoculating tool, move into the middle part of Tube propagation base, beyond the Great Wall cotton seed, then by the test tube after inoculation
Move in constant incubator, in darkroom, incubated 10~15d at 25~35 DEG C can make growth vigorous, the speed of growth
It hurry up, can large scale fermentation production mycelium and extraction active component.Zhang etc. by Phellinus parent species by being inoculated into solid medium
Strain in solid medium, is then inoculated into fluid nutrient medium and cultivates, obtain Phellinus liquid strain by middle lucifuge culture.Solid-state is trained
Supporting component and mass fraction contained by base is：Mulberry wood chips 20%~30%, toothed oak wood chip 40%~50%, wheat bran 20%~27%, brown sugar 1%~
1.5%th, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, land plaster 1~1.5%, moisture content are 60%~63%.This method can make Phellinus
Growth speed is improved up to 30%~40%, is gone out yellow rate and is improved up to 20%, and fructification quality is good, pollute small, repeatable realize.
Zhu etc. uses the matter such as 75~85 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 10~20 parts of wheat bran, 2~10 parts of corn flour, 0.5~1.5 part of sucrose, and addition
The water of amount, is prepared for culture medium.Phellinus strain is inoculated in into above-mentioned culture medium to be cultivated, phellinus igniarius mycelium is obtained, solved
Time-consuming for the artificial culture technique of Phellinus, and cost is high, yields poorly, price high shortcoming.Wang is investigated a kind of Phellinus strain
Fluid nutrient medium preparation method and fermentation process, it is 30~50g/L, KH for 10~25g/L, beancake powder to use glucose2PO4
For 1~5g/L, MgSO4For 1~2g/L, appropriate pH is 5.5~6.5.The medium component of this method is simple, and formula and proportioning are closed
Reason, can promote mycelial growth to develop, and improve strain activity, so as to improve mycelium production, dry mycelium production is up to 13.5g/L
More than.
The method that Phellinus thalline is cultivated using above-mentioned culture medium, all achieved in terms of Phellinus thalline is cultivated it is certain into
Effect, but there are common defects in the stability of strain survival rate and quality, a major reason is that strain is difficult in the medium
To be uniformly distributed, it is also uneven to the nutrient absorption in culture medium, can locally be cultured base and accelerate the ripening, make its aging, or even extremely
Die, this significantly reduces the production efficiency of Phellinus strain and product quality, influence the medical value of Phellinus.For such case,
It is contemplated that using the method being inoculated with advance, promote Phellinus strain in the medium can stable and uniform disperse, while focusing on training
Support trophic component and the proportioning of base, it is ensured that Phellinus strain can be absorbed to sufficient, balanced, stable nutrition from culture medium, so that
Its survival rate and quality stability are improved, the stable cultivation of scale is realized.
The content of the invention
It is an object of the invention to provide a kind of stable method for cultivating Phellinus strain of scale, conventional method can be solved
Survival rate is low, the unstable shortcoming of quality, topmost, and creative application zeolite powder is inoculated with advance with diatomaceous complex
Strain, guarantee is provided for strain being uniformly distributed in the medium with uniform pickup nutrition.
Concrete technical scheme of the present invention is as follows：
A kind of stable method for cultivating Phellinus strain of scale, by by high-temperature sterilization after zeolite powder and composite diatomite, utilizing
Liquid nitrogen is quickly cooled to certain temperature, accesses Phellinus strain on zeolite powder and diatomaceous complex in desinfection chamber.Then use
Water, mulberry tree wood sawdust, carbon source, nitrogen source, phosphate fertilizer, potash fertilizer, magnesium sulfate and agar powder prepared media, and gone out using high steam
Bacterium.Load waffle slab after the complex for being vaccinated with Phellinus bacterium is mixed in proportion with Nutrient medium again, with Plastic Package, be put into culture
Interior, controls the temperature and relative humidity of culturing room, and keeps dark state, and uniform bacterium is can obtain after culture certain time
Silk.What is prepared comprises the following steps that：
（1）Zeolite powder and diatomite are mixed to prepare complex by certain mass ratio, then using high-temperature sterilization, then liquid is used
Nitrogen is cooled down, and after temperature is reduced to 20 ~ 25 DEG C, is transferred in desinfection chamber, and Phellinus strain is accessed on complex；
（2）Culture medium is prepared by certain component ratio, the basis of culture medium includes water, mulberry tree wood sawdust, carbon source, nitrogen
Source, phosphate fertilizer, potash fertilizer, magnesium sulfate and agar powder, then sterilize at certain temperature and pressure, obtain aseptic culture medium；
（3）By step（1）The complex and step that are vaccinated with Phellinus strain of gained（2）Obtained culture medium is mixed in proportion
Afterwards, load waffle slab, with Plastic Package, be put into culturing room, under certain temperature, relative humidity and pH value, keep dark
After state, 25 ~ 30d of culture, you can obtain uniform mycelia.
It is preferred that, step（1）The zeolite powder is 3 with diatomaceous mass ratio：2~2：1；
It is preferred that, step（1）The temperature of the high-temperature sterilization is 200 ~ 220 DEG C；
It is preferred that, step（2）The carbon source is glucose, maltose, sucrose or mannitol；
It is preferred that, step（2）The nitrogen source is organic nitrogen source, such as peptone, beancake powder or wheat bran；
It is preferred that, step（2）The phosphate fertilizer is potassium dihydrogen phosphate or dipotassium hydrogen phosphate with potash fertilizer；
It is preferred that, step（2）The parts by weight of each composition of culture medium are 5 ~ 10 parts of mulberry tree wood sawdust, 2 ~ 3 parts of agar powder, carbon source
2 ~ 3 parts, 4 ~ 6 parts of nitrogen source, 1 ~ 2 part of phosphate fertilizer potash fertilizer, 0.5 ~ 1 part of magnesium sulfate, 8-10 parts of water；
It is preferred that, step（2）The temperature of the high pressure steam sterilization is 121 DEG C, and pressure is 0.1MPa；
It is preferred that, step（2）The time of the high pressure steam sterilization is to be not less than 2h；
It is preferred that, step（3）The mixing quality ratio of the complex and culture medium is 0.2:1~0.3:1；
It is preferred that, step（3）The temperature control is 25 ~ 30 DEG C, and relative humidity control is 80% ~ 85%, and pH value control is 5 ~ 7.
Phellinus growing environment is special, and its breeding strain difficulty is big, and the breeding method of routine techniques, strain survival rate is low, matter
Amount is unstable, it is difficult to realize large-scale cultivation.The method applied in the present invention, by by high temperature after zeolite powder and composite diatomite
Sterilizing, be quickly cooled to certain temperature using liquid nitrogen, be transferred in desinfection chamber, by Phellinus strain access zeolite powder with it is diatomaceous multiple
On zoarium.Then mulberry tree wood sawdust, carbon source, nitrogen source, phosphate fertilizer, potash fertilizer, magnesium sulfate and agar powder are added to the water well mixed,
Culture medium is prefabricated into, and uses high pressure steam sterilization.Agar powder plays a part of adjusting viscosity so that various nutritional ingredients can be
It is even to be stably dispersed in culture medium.Load waffle slab after the complex for being vaccinated with Phellinus bacterium is mixed in proportion with Nutrient medium again,
With Plastic Package, it is put into culturing room, controls the temperature and relative humidity of culturing room, and keeps dark state, the timing of culture one
Between after i.e. can obtain uniform mycelia.For Phellinus strain, using starch, fructose or corn flour as carbon source, with dusty yeast or
Inorganic matter is as nitrogen source, and effect is undesirable, therefore the present invention uses glucose, maltose, sucrose or mannitol for carbon source, adopts
It is nitrogen source with peptone, beancake powder or wheat bran, mycelial growth can be made very fast, bacterium colony is more dense.Zeolite powder is made with diatomite
Complex be inoculated with strain in advance, strain can be made to be uniformly dispersed, be of the invention to the nutritional ingredient uniform pickup in culture medium
The advantage protruded the most.
A kind of stable method for cultivating Phellinus strain of scale, compared with prior art, the characteristics of it is protruded and excellent
Effect is：
1. the present invention is inoculated with strain in advance using zeolite powder and diatomaceous complex, preferably strain is protected to be sought by the external world
Support the unstable interference of base, reduce strain it is scattered uneven, it is local accelerated the ripening by Nutrient medium, the risk of too fast growth or death, into
Motility rate is high；
It is that growth provides safeguard 2. each nutritional ingredient of the prefabricated culture medium of the present invention is balanced, and its growing environment is homogeneous, has
Beneficial to the Phellinus strain for cultivating steady quality；
3. operating process of the present invention is simple and easy to apply, it is easy to control, Phellinus strain is cultivated suitable for extensive.
Embodiment
Below by way of embodiment, the present invention is described in further detail, but this should not be interpreted as to the present invention
Scope be only limitted to following example.In the case where not departing from above method thought of the present invention, according to ordinary skill
Various replacements or change that knowledge and customary means are made, should be included in the scope of the present invention.
Embodiment 1
A kind of stable method for cultivating Phellinus strain of scale, the detailed process that it cultivates Phellinus strain is as follows：
15kg zeolite powders and 10kg diatomite are mixed to prepare complex, the high-temperature sterilization at 210 DEG C, then carried out with liquid nitrogen cold
But, after temperature is reduced to 25 DEG C, it is transferred in desinfection chamber, Phellinus strain is accessed on complex；Then 5kg mulberry tree sawmilling is taken
Bits, 3kg glucose, 5kg peptones, 2kg potassium dihydrogen phosphates, 0.5kg magnesium sulfate and 3kg agar powders, are mixed simultaneously with 81.5kg water
Stir, be then 121 DEG C in temperature, pressure is under 0.1MPa, steam sterilizing 2h obtains aseptic culture medium；Then will
25kg is vaccinated with after the complex of Phellinus strain mixes with obtained 100kg aseptic culture mediums, is loaded waffle slab, is used plastic seal
Dress, is put into culturing room, temperature be 30 DEG C, relative humidity be that 80%, pH value is under conditions of 6, to keep dark state, culture
After 28d, you can obtain uniform mycelia.
Through actual verification, the Phellinus strain survival rate that embodiment 1 is cultivated is high, and mycelial growth rate is very fast, steady quality,
Large-scale production can be achieved.The survival rate of Phellinus strain and the data of mycelial growth rate are as shown in table 1 in embodiment 1.
Embodiment 2
A kind of stable method for cultivating Phellinus strain of scale, the detailed process that it cultivates Phellinus strain is as follows：
12kg zeolite powders and 8kg diatomite are mixed to prepare complex, the high-temperature sterilization at 200 DEG C, then cooled down with liquid nitrogen,
After temperature is reduced to 20 DEG C, it is transferred in desinfection chamber, Phellinus strain is accessed on complex；Then take 8kg mulberry treies wood sawdust,
2kg maltose, 4kg beancake powders, 1kg dipotassium hydrogen phosphates, 1kg magnesium sulfate and 2kg agar powders, are mixed and stirred for 10kg water
It is even, it is then 121 DEG C in temperature, pressure is under 0.1MPa, steam sterilizing 2h obtains aseptic culture medium；Then 20kg is inoculated with
After the complex of Phellinus strain is mixed with obtained 100kg aseptic culture mediums, load waffle slab, with Plastic Package, be put into training
Support interior, temperature be 25 DEG C, relative humidity be that 80%, pH value is under conditions of 5, to keep after dark state, culture 30d, you can
Obtain uniform mycelia.
Through actual verification, the Phellinus strain survival rate that embodiment 2 is cultivated is high, and mycelial growth rate is very fast, steady quality,
Large-scale production can be achieved.The survival rate of Phellinus strain and the data of mycelial growth rate are as shown in table 1 in embodiment 2.
Embodiment 3
A kind of stable method for cultivating Phellinus strain of scale, the detailed process that it cultivates Phellinus strain is as follows：
20kg zeolite powders and 10kg diatomite are mixed to prepare complex, the high-temperature sterilization at 220 DEG C, then carried out with liquid nitrogen cold
But, after temperature is reduced to 25 DEG C, it is transferred in desinfection chamber, Phellinus strain is accessed on complex；Then 7kg mulberry tree sawmilling is taken
Bits, 3kg sucrose, 5kg wheat brans, 1kg potassium dihydrogen phosphates, 0.5kg magnesium sulfate and 3kg agar powders, are mixed and stirred for 8.5kg water
It is even, it is then 121 DEG C in temperature, pressure is under 0.1MPa, steam sterilizing 2h obtains aseptic culture medium；Then 30kg is inoculated with
After the complex of Phellinus strain is mixed with obtained 100kg aseptic culture mediums, load waffle slab, with Plastic Package, be put into training
Support interior, temperature be 25 DEG C, relative humidity be that 85%, pH value is under conditions of 7, to keep after dark state, culture 28d, you can
Obtain uniform mycelia.
Through actual verification, the Phellinus strain survival rate that embodiment 3 is cultivated is high, and mycelial growth rate is very fast, steady quality,
Large-scale production can be achieved.The survival rate of Phellinus strain and the data of mycelial growth rate are as shown in table 1 in embodiment 3.
Embodiment 4
A kind of stable method for cultivating Phellinus strain of scale, the detailed process that it cultivates Phellinus strain is as follows：
15kg zeolite powders and 10kg diatomite are mixed to prepare complex, the high-temperature sterilization at 210 DEG C, then carried out with liquid nitrogen cold
But, after temperature is reduced to 25 DEG C, it is transferred in desinfection chamber, Phellinus strain is accessed on complex；Then 10kg mulberry treies are taken to saw
Wood chip, 3kg mannitol, 4kg peptones, 2kg dipotassium hydrogen phosphates, 0.5kg magnesium sulfate and 2kg agar powders, are mixed simultaneously with 9.5kg water
Stir, be then 121 DEG C in temperature, pressure is under 0.1MPa, steam sterilizing 2h obtains aseptic culture medium；Then will
25kg is vaccinated with after the complex of Phellinus strain mixes with obtained 100kg aseptic culture mediums, is loaded waffle slab, is used plastic seal
Dress, is put into culturing room, temperature be 30 DEG C, relative humidity be that 80%, pH value is under conditions of 6, to keep dark state, culture
After 25d, you can obtain uniform mycelia.
Through actual verification, the Phellinus strain survival rate that embodiment 4 is cultivated is high, and mycelial growth rate is very fast, steady quality,
Large-scale production can be achieved.The survival rate of Phellinus strain and the data of mycelial growth rate are as shown in table 1 in embodiment 4.
Embodiment 5
A kind of stable method for cultivating Phellinus strain of scale, the detailed process that it cultivates Phellinus strain is as follows：
20kg zeolite powders and 10kg diatomite are mixed to prepare complex, the high-temperature sterilization at 200 DEG C, then carried out with liquid nitrogen cold
But, after temperature is reduced to 20 DEG C, it is transferred in desinfection chamber, Phellinus strain is accessed on complex；Then 8kg mulberry tree sawmilling is taken
Bits, 2kg glucose, 6kg beancake powders, 1kg dipotassium hydrogen phosphates, 1kg magnesium sulfate and 2kg agar powders, are mixed and stirred for 8kg water
It is even, it is then 121 DEG C in temperature, pressure is under 0.1MPa, steam sterilizing 2h obtains aseptic culture medium；Then 30kg is inoculated with
After the complex of Phellinus strain is mixed with obtained 100kg aseptic culture mediums, load waffle slab, with Plastic Package, be put into training
Support interior, temperature be 30 DEG C, relative humidity be that 85%, pH value is under conditions of 5, to keep after dark state, culture 30d, you can
Obtain uniform mycelia.
Through actual verification, the Phellinus strain survival rate that embodiment 5 is cultivated is high, and mycelial growth rate is very fast, steady quality,
Large-scale production can be achieved.The survival rate of Phellinus strain and the data of mycelial growth rate are as shown in table 1 in embodiment 5.
Embodiment 6
A kind of stable method for cultivating Phellinus strain of scale, the detailed process that it cultivates Phellinus strain is as follows：
15kg zeolite powders and 10kg diatomite are mixed to prepare complex, the high-temperature sterilization at 220 DEG C, then carried out with liquid nitrogen cold
But, after temperature is reduced to 25 DEG C, it is transferred in desinfection chamber, Phellinus strain is accessed on complex；Then 6kg mulberry tree sawmilling is taken
Bits, 2kg glucose, 5kg wheat brans, 2kg potassium dihydrogen phosphates, 0.5kg magnesium sulfate and 3kg agar powders, are mixed and stirred for 9kg water
It is even, it is then 121 DEG C in temperature, pressure is under 0.1MPa, steam sterilizing 2h obtains aseptic culture medium；Then 25kg is inoculated with
After the complex of Phellinus strain is mixed with obtained 100kg aseptic culture mediums, load waffle slab, with Plastic Package, be put into training
Support interior, temperature be 25 DEG C, relative humidity be that 80%, pH value is under conditions of 7, to keep after dark state, culture 30d, you can
Obtain uniform mycelia.
Through actual verification, the Phellinus strain survival rate that embodiment 6 is cultivated is high, and mycelial growth rate is very fast, steady quality,
Large-scale production can be achieved.The survival rate of Phellinus strain and the data of mycelial growth rate are as shown in table 1 in embodiment 6.
Table 1：
| Specific embodiment | Strain survival rate（%） | Mycelial growth rate（cm/d） |
| Embodiment 1 | 82.5 | 1.15 |
| Embodiment 2 | 84.6 | 1.21 |
| Embodiment 3 | 83.1 | 1.36 |
| Embodiment 4 | 82.8 | 1.27 |
| Embodiment 5 | 83.4 | 1.33 |
| Embodiment 6 | 81.5 | 1.28 |
Claims (10)
1. a kind of stable method for cultivating Phellinus strain of scale, it is characterised in that by by after zeolite powder and composite diatomite
High-temperature sterilization, certain temperature is quickly cooled to using liquid nitrogen, desinfection chamber by Phellinus strain access zeolite powder with it is diatomaceous multiple
On zoarium；Then water, mulberry tree wood sawdust, carbon source, nitrogen source, phosphate fertilizer, potash fertilizer, magnesium sulfate and agar powder prepared media are used, and is adopted
Use high pressure steam sterilization；Load waffle slab after the complex for being vaccinated with Phellinus bacterium is mixed in proportion with Nutrient medium again, use plastics
Encapsulation, is put into culturing room, controls the temperature and relative humidity of culturing room, and keeps dark state, after culture certain time i.e.
It can obtain uniform mycelia；What is prepared comprises the following steps that：
（1）Zeolite powder and diatomite are mixed to prepare complex by certain mass ratio, then using high-temperature sterilization, then liquid is used
Nitrogen is cooled down, and after temperature is reduced to 20 ~ 25 DEG C, is transferred in desinfection chamber, and Phellinus strain is accessed on complex；
（2）Culture medium is prepared by certain component ratio, the basis of culture medium includes water, mulberry tree wood sawdust, carbon source, nitrogen
Source, phosphate fertilizer, potash fertilizer, magnesium sulfate and agar powder, then sterilize at certain temperature and pressure, obtain aseptic culture medium；
（3）By step（1）The complex and step that are vaccinated with Phellinus strain of gained（2）Obtained culture medium is mixed in proportion
Afterwards, load waffle slab, with Plastic Package, be put into culturing room, under certain temperature, relative humidity and pH value, keep dark
After state, 25 ~ 30d of culture, you can obtain uniform mycelia.
2. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（1）Institute
It is 3 that zeolite powder, which is stated, with diatomaceous mass ratio：2~2：1.
3. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（1）Institute
The temperature for stating high-temperature sterilization is 200 ~ 220 DEG C.
4. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（2）Institute
Carbon source is stated for glucose, maltose, sucrose or mannitol.
5. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（2）Institute
Nitrogen source is stated for organic nitrogen source peptone, beancake powder or wheat bran.
6. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（2）Institute
It is potassium dihydrogen phosphate or dipotassium hydrogen phosphate that phosphate fertilizer, which is stated, with potash fertilizer.
7. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（2）Institute
The parts by weight for stating each composition of culture medium are 5 ~ 10 parts of mulberry tree wood sawdust, 2 ~ 3 parts of agar powder, 2 ~ 3 parts of carbon source, 4 ~ 6 parts of nitrogen source, phosphate fertilizer
1 ~ 2 part of potash fertilizer, 0.5 ~ 1 part of magnesium sulfate, 8-10 parts of water.
8. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（2）Institute
The temperature for stating high pressure steam sterilization is 121 DEG C, and pressure is 0.1MPa；The time of the high pressure steam sterilization is to be not less than 2h.
9. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（3）Institute
The mixing quality ratio for stating complex and culture medium is 0.2:1~0.3:1.
10. a kind of method that scale stabilization cultivates Phellinus strain according to claim 1, it is characterised in that：Step（3）Institute
It is 25 ~ 30 DEG C to state temperature control, and relative humidity control is 80% ~ 85%, and pH value control is 5 ~ 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710455750.1A CN107142216B (en) | 2017-06-16 | 2017-06-16 | Method for stably cultivating phellinus igniarius strains on large scale |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710455750.1A CN107142216B (en) | 2017-06-16 | 2017-06-16 | Method for stably cultivating phellinus igniarius strains on large scale |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107142216A true CN107142216A (en) | 2017-09-08 |
| CN107142216B CN107142216B (en) | 2021-02-09 |
Family
ID=59781410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710455750.1A Active CN107142216B (en) | 2017-06-16 | 2017-06-16 | Method for stably cultivating phellinus igniarius strains on large scale |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107142216B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108782018A (en) * | 2018-06-28 | 2018-11-13 | 安康学院 | A kind of cultural method of Phellinus strain |
| CN109168958A (en) * | 2018-10-11 | 2019-01-11 | 安康学院 | A kind of Phellinus cultural method |
| CN110337986A (en) * | 2019-07-10 | 2019-10-18 | 杭州丝绸之路文化艺术有限公司 | A kind of Spawn incubation method of Phellinus |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816256A (en) * | 2009-02-27 | 2010-09-01 | 上海市农业科学院 | Method for cultivating microbial strain of phellinus linteus and planting phellinus linteus |
| CN102212485A (en) * | 2011-05-09 | 2011-10-12 | 中国农业科学院农业资源与农业区划研究所 | Trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases |
| WO2012018422A1 (en) * | 2010-08-03 | 2012-02-09 | Ferro Corporation | Polymer composite foams |
| CN104151037A (en) * | 2014-07-12 | 2014-11-19 | 合肥奕涵农牧科技有限公司 | Hypsizigus marmoreus cultivation material taking mulberry sawdust as raw material and preparation method thereof |
| CN105850501A (en) * | 2016-04-21 | 2016-08-17 | 富源县好源农业综合有限责任公司 | Method of planting Poria cocos using pine tree stumps |
-
2017
- 2017-06-16 CN CN201710455750.1A patent/CN107142216B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816256A (en) * | 2009-02-27 | 2010-09-01 | 上海市农业科学院 | Method for cultivating microbial strain of phellinus linteus and planting phellinus linteus |
| WO2012018422A1 (en) * | 2010-08-03 | 2012-02-09 | Ferro Corporation | Polymer composite foams |
| CN102212485A (en) * | 2011-05-09 | 2011-10-12 | 中国农业科学院农业资源与农业区划研究所 | Trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases |
| CN104151037A (en) * | 2014-07-12 | 2014-11-19 | 合肥奕涵农牧科技有限公司 | Hypsizigus marmoreus cultivation material taking mulberry sawdust as raw material and preparation method thereof |
| CN105850501A (en) * | 2016-04-21 | 2016-08-17 | 富源县好源农业综合有限责任公司 | Method of planting Poria cocos using pine tree stumps |
Non-Patent Citations (2)
| Title |
|---|
| 姜明: "不同 pH 值和培养基对桑黄菌丝生长的影响", 《北方园艺》 * |
| 秦俊哲: "桑黄菌丝的最适培养条件研究", 《食用菌》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108782018A (en) * | 2018-06-28 | 2018-11-13 | 安康学院 | A kind of cultural method of Phellinus strain |
| CN109168958A (en) * | 2018-10-11 | 2019-01-11 | 安康学院 | A kind of Phellinus cultural method |
| CN109168958B (en) * | 2018-10-11 | 2020-11-24 | 安康学院 | Phellinus igniarius cultivation method |
| CN110337986A (en) * | 2019-07-10 | 2019-10-18 | 杭州丝绸之路文化艺术有限公司 | A kind of Spawn incubation method of Phellinus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107142216B (en) | 2021-02-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101703214A (en) | Lucid Ganoderma hypra powder or Lucid Ganoderma tea and double fermentation process | |
| CN103734482B (en) | The production method of a kind of feed addictive " mortierella Diding culture " | |
| CN101822170A (en) | Method for producing Anrodia camphorata mycelia based on solid-state surface culture | |
| CN103289937B (en) | Method for producing bacillus laterosporus live bacteria by high density solid fermentation | |
| CN106011022B (en) | A kind of rose yellow streptomycete solid fermentation culture medium and its preparation and fermentation process | |
| CN102391034A (en) | Formula and preparation method of Pleurotus eryngii liquid strain culture medium | |
| CN105441337A (en) | Preparation method of cultivated strain of tremella aurantialba | |
| CN109566268A (en) | Effective original seed of a kind of gold ear and preparation method thereof | |
| CN107142216A (en) | A kind of stable method for cultivating Phellinus strain of scale | |
| CN103250556A (en) | Method for cultivating rhodiola rosea bag material healthcare pleurotus nebrodensis | |
| CN102964170A (en) | Formula and preparation method of agaricus bisporus liquid spawn | |
| CN102381896B (en) | Crab-flavor mushroom liquid strain medium formula and preparation method thereof | |
| CN107384824A (en) | Stalk rotten agent soon | |
| CN104261942A (en) | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain | |
| CN103314770A (en) | Fluid culture preparing method for bamboo fungus | |
| CN104087539B (en) | Streptomyces microflavus solid fermentation culture medium, preparation method and fermentation process thereof | |
| CN109234176A (en) | Cordyceps sinensis mother culture media and preparation method thereof | |
| CN103881930A (en) | Solid culture medium and culture method of beauveria | |
| CN105985913A (en) | Formula and preparation method of pleurotus eryngii liquid strain medium | |
| CN102964156A (en) | Culture medium formula and preparation method of Pleurotus nebrodensis liquid spawn | |
| CN107094499B (en) | Method for stably cultivating phellinus igniarius by using grain powder as culture medium | |
| CN103858659A (en) | Bag cultivation method of black fungi | |
| CN111264303B (en) | Lyophyllum fumosoroseum cultivar and preparation method thereof | |
| CN106831037A (en) | A kind of edible fungus liquid culture growth medium and preparation method thereof | |
| CN103849592A (en) | Production method for streptomycete spores |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210112 Address after: 321025 houshanwan, shangshao village, bailongqiao Town, Wucheng District, Jinhua City, Zhejiang Province Applicant after: JINHUA TINGMEI TECHNOLOGY Co.,Ltd. Address before: 610091, Sichuan, Chengdu province Qingyang dragon industrial port, East Sea 4 Applicant before: CHENGDU NEW KELI CHEMICAL SCIENCE Co.,Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |