CN103250638A - Chrysanthemum tissue culture method - Google Patents

Abstract

The invention discloses a chrysanthemum tissue culture method, which comprises the following steps of: tissue culture and water control: during the excessive growth period of chrysanthemum, carrying out water control, so that the chrysanthemum is devitalized; needle piercing: piercing the stem of the lower end of a leaf at a growing point by using a sterilized needle; and fertilization. According to the chrysanthemum tissue culture method disclosed by the invention, the inherent properties and features of original varieties are kept, the reproduction speed is high, the land resource is saved, and an effect of no virus and no pollution can be achieved; through the methods such as needle piercing, dwarfing chrysanthemum can be obtained, thereby enhancing the ornamental value; and through the addition of various fertilizers, the self nutritional value of chrysanthemum is enhanced, and the chrysanthemum is beneficial to the physical health in case of being used for making tea.

Description

A kind of chrysanthemum tissue is cultivated cultivation method

Technical field

The present invention relates generally to a kind of chrysanthemum tissue and cultivates cultivation method, belongs to field of planting.

Background technology

Cultivating chrysanthemum, often to control its height, the strain shape attractive in appearance to obtain compact, have only short just can the making of strain to spend greatly, in great numbers, suitable indoor ornaments, the method that traditional chrysanthemum is downgraded is to postpone cutting time mostly, pinching, regularly spray chlormequat etc., complicated operation, the family planting chrysanthemum is also more and more general now, and the chrysanthemum of kind not only can be used for viewing and admiring, can also be used to making tea etc., therefore the nutritive value that strengthens chrysanthemum also is necessary, cultivates by tissue and obtains the chrysanthemum seedling, has not only kept intrinsic properties and the characteristic of original kind, can also economize the land resource, and virus-free.

Summary of the invention

The object of the invention is exactly in order to provide a kind of chrysanthemum to downgrade processing method.

The present invention is achieved by the following technical solutions:

A kind of chrysanthemum tissue is cultivated cultivation method, and it may further comprise the steps:

(1) tissue is cultivated: get the chrysanthemum spray as explant, use aseptic water washing 4-5 time earlier, with the mercuric chloride solution sterilization of 0.1-0.2%, being inoculated into afterwards in the medium then, is to carry out aseptic culture under 20-22 ℃ in temperature, to taking root, seedling, seedling is taken out the back clean root with clear water, be transplanted in the matrix that sterilization vermiculite and perlite by 1:2-3 mix and cultivate, general incubation time is 5-15 days, can be transplanted in the soil after waiting seedling to become strong;

The weight portion (g) of contained each raw material is in every liter of described medium:

Agar 10-12, dusty yeast 4-5, sucrose 20-30, diammonium hydrogen citrate 1-3, potassium dihydrogen phosphate 2-3, ferrous sulfate heptahydrate 0.2-0.4, remaining be distilled water;

(2) transplant: in the time of transplanting, nutrients is admixed in the basin soil uniformly, nutraceutical consumption is the 2-3% of basin soil, and described nutrients is made up of the raw material mixing grinding of following weight parts:

Silkworm excrement 8-10, brewer's grains 6-8, xylitol slag 2-4, weathered coal 1-2;

(3) control water: chrysanthemum excessive growth period control water, here make it to beat;

(4) acupuncture: in the blade lower end of growing point, use the needle-penetration stem through sterilization;

(5) fertilising: every basin application of organic fertilizers 0.4-0.6kg, bloomed previous month, every 3-5 days, urea, the potassium dihydrogen phosphate of 0.04-0.06%, the mixed liquor that can impose 0.1-0.2% were made liquid manure;

Described fertilizer is made up of the raw material of following weight parts:

Edible fungi residues 30-40, poultry-dung 30-40, distiller's dried grain 8-10, system chaff 8-10, bagasse 4-6, alum powder 2-4, rapeseed dregs 4-5;

Above-mentioned raw materials is mixed the organic material composting agent that grinds back adding 1-3%, add water-water reactor and put fermentation, namely get described fertilizer.

Advantage of the present invention is:

Chrysanthemum tissue of the present invention is cultivated cultivation method, not only keeps intrinsic properties and the characteristic of original kind, and reproduction speed is fast, economize the land resource, can be virus-free, pollution-free, by methods such as acupunctures, the chrysanthemum that can obtain to downgrade, strengthen sight, by the various fertilizer that add, strengthened the nutritive value of chrysanthemum self again, be used for making tea body health benefits.

Embodiment

Embodiment 1

A kind of chrysanthemum tissue is cultivated cultivation method, and it may further comprise the steps:

(1) tissue is cultivated: get the chrysanthemum spray as explant, use aseptic water washing 5 times earlier, with the mercuric chloride solution sterilization of 0.1-0.2%, being inoculated into afterwards in the medium then, is to carry out aseptic culture under 22 ℃ in temperature, to taking root, seedling, seedling is taken out the back clean root with clear water, be transplanted in the matrix that sterilization vermiculite and perlite by 1:3 mix and cultivate, general incubation time is 15 days, can be transplanted in the soil after waiting seedling to become strong;

The weight portion (g) of contained each raw material is in every liter of described medium:

Agar 12, dusty yeast 5, sucrose 30, diammonium hydrogen citrate 3, potassium dihydrogen phosphate 3, ferrous sulfate heptahydrate 0.4, remaining be distilled water;

(2) transplant: in the time of transplanting, nutrients is admixed in the basin soil uniformly, nutraceutical consumption is 3% of basin soil, and described nutrients is made up of the raw material mixing grinding of following weight parts:

Silkworm excrement 10, brewer's grains 8, xylitol slag 4, weathered coal 2;

(3) control water: chrysanthemum excessive growth period control water, here make it to beat;

(4) acupuncture: in the blade lower end of growing point, use the needle-penetration stem through sterilization;

(5) fertilising: every basin application of organic fertilizers 0.6kg, it is previous month to bloom, and every 5 days, can impose 0.2% urea, 0.06% potassium dihydrogen phosphate, mixed liquor made liquid manure;

Described fertilizer is made up of the raw material of following weight parts:

Edible fungi residues 40, poultry-dung 40, distiller's dried grain 10, system chaff 10, bagasse 6, alum powder 4, rapeseed dregs 5;

Above-mentioned raw materials is mixed the organic material composting agent that grinds back adding 1-3%, add water-water reactor and put fermentation, namely get described fertilizer.

The chrysanthemum plant height that the present invention produces is no more than 35 centimetres.The chrysanthemum quality that the present invention grows is good, it is dense to grow, growing power is strong, reception luminosity height, the diseases prevention performance is good, belongs to 100% natural green chrysanthemum, effectively overcome because the deficiency of various nutrition fertilizer, easily cause chrysanthemum blade face Huang, the rareness of blooming, longer chrysanthemum stature little, premunition is poor, easily suffers from the defective of various damage by disease and insect.

Claims (1)

1. a chrysanthemum tissue is cultivated cultivation method, it is characterized in that it may further comprise the steps:

(1) tissue is cultivated: get the chrysanthemum spray as explant, use aseptic water washing 4-5 time earlier, with the mercuric chloride solution sterilization of 0.1-0.2%, being inoculated into afterwards in the medium then, is to carry out aseptic culture under 20-22 ℃ in temperature, to taking root, seedling, seedling is taken out the back clean root with clear water, be transplanted in the matrix that sterilization vermiculite and perlite by 1:2-3 mix and cultivate, general incubation time is 5-15 days, can be transplanted in the soil after waiting seedling to become strong;

The weight portion (g) of contained each raw material is in every liter of described medium:

Agar 10-12, dusty yeast 4-5, sucrose 20-30, diammonium hydrogen citrate 1-3, potassium dihydrogen phosphate 2-3, ferrous sulfate heptahydrate 0.2-0.4, remaining be distilled water;

(2) transplant: in the time of transplanting, nutrients is admixed in the basin soil uniformly, nutraceutical consumption is the 2-3% of basin soil, and described nutrients is made up of the raw material mixing grinding of following weight parts:

Silkworm excrement 8-10, brewer's grains 6-8, xylitol slag 2-4, weathered coal 1-2;

(3) control water: chrysanthemum excessive growth period control water, here make it to beat;

(4) acupuncture: in the blade lower end of growing point, use the needle-penetration stem through sterilization;

(5) fertilising: every basin application of organic fertilizers 0.4-0.6kg, bloomed previous month, every 3-5 days, urea, the potassium dihydrogen phosphate of 0.04-0.06%, the mixed liquor that can impose 0.1-0.2% were made liquid manure;

Described fertilizer is made up of the raw material of following weight parts:

Edible fungi residues 30-40, poultry-dung 30-40, distiller's dried grain 8-10, system chaff 8-10, bagasse 4-6, alum powder 2-4, rapeseed dregs 4-5;

Above-mentioned raw materials is mixed the organic material composting agent that grinds back adding 1-3%, add water-water reactor and put fermentation, namely get described fertilizer.