CN106688884A - Chrysanthemum cultivation method - Google Patents
Chrysanthemum cultivation method Download PDFInfo
- Publication number
- CN106688884A CN106688884A CN201611018437.3A CN201611018437A CN106688884A CN 106688884 A CN106688884 A CN 106688884A CN 201611018437 A CN201611018437 A CN 201611018437A CN 106688884 A CN106688884 A CN 106688884A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- chrysanthemum
- explant
- pinching
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a chrysanthemum cultivation method. According to the technical scheme of the invention, the chrysanthemum cultivation method sequentially comprises the following steps: (1) selecting and sterilizing explants; (2) preparing a culture medium; (3) cultivating the explants indoors; (4) transplanting outdoors; (5) pinching; (6) applying a fertilizer. The chrysanthemum cultivation method has the advantages of inhibiting bacterial virus from invading plants, resisting plant diseases and insect pests, enabling the chrysanthemum to grow quickly, increasing the survival rate and increasing economic benefit. In addition, auxin and cytokinin are increased in the culture medium, the mass concentration of the cytokinin is increased, formation of buds is benefited, the mass concentration of the auxin is increased, formation of roots is benefited, when the cytokinin and the auxin are in a certain balanced mass concentration, formation of the callus tissue is benefited.
Description
Technical field
The present invention relates to a kind of cultural method of chrysanthemum.
Background technology：
Chrysanthemum, is used as medicine with flower, is large conventional Chinese medicine, the adenine of glycosides containing chrysanthemum, choline, stachydrine, amino acid, robinin,
The compositions such as borneol level resacetophenone.The traditional Chinese medical science thinks its cold nature, bitter, dry.Nourish the liver to improve visual acuity, wind and heat dispersing the effects such as,
Cure mainly the diseases such as cold, fever, headache, tinnitus, red eye, swell pain, abscess of throat.
Chrysanthemum is liked gentle nice and cool weather and is faced south, slightly dry environment.Can be cold-resistant, flood afraid of water.It is raw in the environment for covering
Length is bad.Growth period requires that soil is slightly moistened, excessively arid, and plant branches out less, and development is slow, yields poorly, especially the nearly florescence,
Water shortage is unable to, is otherwise greatly reduced number of flowers.But excess moisture, then easily cause rotten dead seedling.Chrysanthemum happiness fertilizer, in loose fertilizer
The fertile, well-grown containing humus is abundant, in well-drained folder sandy soil, spends many yield high.Soil is with neutrality to subacidity or micro-
Alkalescence is suitable.All soil sticks the big plot of weight, hypsography low-lying, impeded drainage, saline alkali and should not cultivate.Avoid continuous cropping, exist year after year
Planted on same soil, pest and disease damage is more, and yield and quality significantly decline.Chrysanthemum belongs to short-day plant, and counterglow length is anti-
Should be sensitive, it is desirable to be no more than dailyThe illumination of hour, can just budding and bloom.
However, in the cultivation of chrysanthemum, the training initial stage is especially planted, it is most easily sick.It is subject to aleyrodid, aphid and the chrysanthemum tip
Worm, waits the harm of insect.So that its bulb occurs defect, wound can also ultimately result in kind of a scutellum rot by fungi, virus infection,
This is greatly lowered the survival rate of chrysanthemum, reduces economic benefit.
In addition, in existing implantation methods, all thinking that plantation chrysanthemum is not tight to soil requirement, will not make any to soil
Treatment, so in spite of illness, will can easily cause the chrysanthemum of plantation some common diseases, such as root rot, frost occur in itself due to soil
Mildew, brown spot etc., are also to be processed according to the method for conventional spray agricultural chemicals after there is disease pest, and environment is polluted, and
Soil texture can be also destroyed, food safety and sanitation standard is not met.Therefore, it should provide a kind of new technical scheme solution above-mentioned
Problem.
The content of the invention：
The invention aims to overcome the shortcomings of the above, there is provided one kind can suppress bacterial virus and invade plant, disease-resistant
Insect pest, the cultural method that can be mushroomed out chrysanthemum, increase a kind of chrysanthemum that survival rate is high, increase economic efficiency.
The purpose of the present invention is achieved through the following technical solutions,
A kind of cultural method of chrysanthemum, it is characterised in that comprise the following steps：
(1), the selection of explant and sterilizing：Stem apex, stem section and lateral bud from chrysanthemum as explant, in 5~August,
The chrysanthemum tender stem young sprout of robust growth is intercepted, the stem section and the lateral bud of health of 3cm after terminal bud, terminal bud is taken, is cleaned with clear water, then
With 60%-80% 25-35s of Ethanol Treatment, 0.5%-1.5% mercuric chloride processes 3-5min, and aseptic water washing 3-5 times is standby,
To stem apex and bud, need to carefully be peeled off under anatomical lens, and the tip of 0.5~1.5mm is cut with knife, be uprightly placed on culture medium,
Stem section is then directly placed on culture medium；
(2), culture medium processed：In parts by weight, its formula is made up of the culture medium following component：N15-P15-K17 1-2
Part, potassium chloride 1-1.5 parts, urea 1-1.5 parts, 1-1.5 parts of calcium nitrate type composite fertilizer N12.2-P0-K0-Ca24.5, EDDHA chelas
0.5-0.8 parts of iron is closed, each composition of the above halves and is made root media, and a certain amount of cell division is added in above-mentioned culture medium
Element and auxin, are configured to differential medium, and the basic element of cell division uses benzylaminopurine, 1-5mg/L, only in differential medium
Middle addition, auxin uses methyl α-naphthyl acetate, and consumption is 0.005-0.015mg/L in differential medium, is used in root media
It is 0.1-0.3mg/L to measure；
(3), indoor culture explant:Explant is initially positioned on differential medium and is grown, entered after differentiation is sprouted
Row squamous subculture, when further culture makes bud grow to 25-35mm, is transferred on root media, it is grown up to whole plant,
4-6d after inoculation explant, cultivates under half-light, awards the 12~16h of illumination of 1400~2000lx, cultivation temperature daily later
It is 23-26 DEG C；
(4), outdoor transplanting：Garden ground requires that the soil is porous, and draining is good, and soil will be made whole good ground through ploughing deeply thin rake
Into high ridge, the culture medium of seedling root is cleaned, is planted on seedbed, starting 2-4d will take cool canopy and spray water in time or spray,
Avoid intense light irradiation and ensure humidity high, illumination progressively can be suitably increased after 1~2d, water daily 1~2 time, summer high-temperature is poured
Water 2~3 times, and weed with a hoe in time and apply fertilizer, ensure seedling healthy growth；
(5) pinching：First time pinching is carried out when height of seedling is to 25cm, second pinching is carried out after 15~20d, then be spaced 15
~20d carries out third time pinching, after third time pinching, 200~300g calcium magnesium phosphate is sprayed to water 30 by every mu to plant blade face
~50kg；
(6) apply fertilizer：After buddingging, to plant foliage-spray foliar fertilizer once, every mu sprays 200~300g to every 8~10d every time
Calcium magnesium phosphate is to 30~50kg of water, and 150~200g potassium sulfates are to 20~50kg of water, when it is fickle in love scatter 2/3 when by harvest.
The present invention has advantages below compared with prior art：
1st, explant is sterilized repeatedly before sowing, is eliminated virus, the bacterium of female parent, improve surviving for transplanting
Rate, further suppress the germination and growth of germ spore, serve the effect for preventing germ from invading plant, reduce courses of infection
Chance.
2nd, auxin and the basic element of cell division are increased in the medium, and the mass concentration for improving the basic element of cell division is conducive to bud
Formation, improve auxin mass concentration, be conducive to the formation of root, both be in certain balance mass concentration when, be conducive to healing
The formation of injured tissue.
Specific embodiment：
In order to deepen the understanding of the present invention, below in conjunction with example, the invention will be further described, and the embodiment is only
For explaining the present invention, it is not intended to limit the scope of the present invention..
Embodiment 1：
A kind of cultural method of chrysanthemum, comprises the following steps：
(1), the selection of explant and sterilizing：Stem apex, stem section and lateral bud from chrysanthemum as explant, in 5~August,
The chrysanthemum tender stem young sprout of robust growth is intercepted, the stem section and the lateral bud of health of 3cm after terminal bud, terminal bud is taken, is cleaned with clear water, then
With 60% Ethanol Treatment 25s, 0.5% mercuric chloride treatment 3min, aseptic water washing 3 times is standby, to stem apex and bud, need to be under anatomical lens
Carefully peel off, and the tip of 0.5mm is cut with knife, be uprightly placed on culture medium, stem section is then directly placed on culture medium；
(2), culture medium processed：In parts by weight, its formula is made up of the culture medium following component：1 part of N15-P15-K17,
1 part of potassium chloride, 1 part of urea, 1 part of calcium nitrate type composite fertilizer N12.2-P0-K0-Ca24.5,0.5 part of EDDHA chelated irons, the above
Each composition halves and is made root media, and a certain amount of basic element of cell division and auxin are added in above-mentioned culture medium, is configured to
Differential medium, the basic element of cell division uses benzylaminopurine, 1mg/L only to be added in differential medium, and auxin uses naphthalene
Acetic acid, consumption is 0.005mg/L in differential medium, and consumption is 0.1mg/L in root media；
(3), indoor culture explant:Explant is initially positioned on differential medium and is grown, entered after differentiation is sprouted
Row squamous subculture, when further culture makes bud grow to 25mm, is transferred on root media, it is grown up to whole plant, connects
4d after explant is planted, is cultivated under half-light, award the illumination 12h of 1400lx daily later, cultivation temperature is 23 DEG C；
(4), outdoor transplanting：Garden ground requires that the soil is porous, and draining is good, and soil will be made whole good ground through ploughing deeply thin rake
Into high ridge, the culture medium of seedling root is cleaned, is planted on seedbed, starting 2-4d will take cool canopy and spray water in time or spray,
Avoid intense light irradiation and ensure humidity high, illumination progressively can be suitably increased after 1d, water daily 1 time, summer high-temperature waters 2 times,
And weed with a hoe and apply fertilizer in time, ensure seedling healthy growth；
(5) pinching：First time pinching is carried out when height of seedling is to 25cm, second pinching is carried out after 15d, then is spaced 15d to enter
Row third time pinching, after third time pinching, 200g calcium magnesium phosphate is sprayed to water 30kg to plant blade face by every mu；
(6) apply fertilizer：After buddingging, every mu sprays 200g calcium magnesium phosphate every time per 8d to plant foliage-spray foliar fertilizer once
To water 30kg, and 150g potassium sulfates are to water 20kg, when it is fickle in love scatter 2/3 when by harvest.
Embodiment two
A kind of cultural method of chrysanthemum, comprises the following steps：
(1), the selection of explant and sterilizing：Stem apex, stem section and lateral bud from chrysanthemum as explant, in 5~August,
The chrysanthemum tender stem young sprout of robust growth is intercepted, the stem section and the lateral bud of health of 3cm after terminal bud, terminal bud is taken, is cleaned with clear water, then
With 70% Ethanol Treatment 30s, 1% mercuric chloride treatment 4min, aseptic water washing 4 times is standby, to stem apex and bud, need to be in anatomical lens bearing
Thin stripping, and the tip of 1mm is cut with knife, uprightly it is placed on culture medium, stem section is then directly placed on culture medium；
(2), culture medium processed：In parts by weight, its formula is made up of the culture medium following component：N15-P15-K17 1.5
Part, 1.3 parts of potassium chloride, 1.3 parts of urea, 1.3 parts of calcium nitrate type composite fertilizer N12.2-P0-K0-Ca24.5, EDDHA chelated irons
0.7 part, each composition of the above halves and is made root media, and a certain amount of basic element of cell division and growth are added in above-mentioned culture medium
Element, is configured to differential medium, and the basic element of cell division uses benzylaminopurine, and 3mg/L is only added in differential medium, raw
Element long uses methyl α-naphthyl acetate, and consumption is 0.1mg/L in differential medium, and consumption is 0.2mg/L in root media；
(3), indoor culture explant:Explant is initially positioned on differential medium and is grown, entered after differentiation is sprouted
Row squamous subculture, when further culture makes bud grow to 25-35mm, is transferred on root media, it is grown up to whole plant,
5d after inoculation explant, cultivates under half-light, awards the illumination 14h of 1700lx daily later, and cultivation temperature is 24 DEG C；
(4), outdoor transplanting：Garden ground requires that the soil is porous, and draining is good, and soil will be made whole good ground through ploughing deeply thin rake
Into high ridge, the culture medium of seedling root is cleaned, is planted on seedbed, starting 2-4d will take cool canopy and spray water in time or spray,
Avoid intense light irradiation and ensure humidity high, illumination progressively can be suitably increased after 2d, water daily 2 times, summer high-temperature waters 3 times,
And weed with a hoe and apply fertilizer in time, ensure seedling healthy growth；
(5) pinching：First time pinching is carried out when height of seedling is to 25cm, second pinching is carried out after 17d, then is spaced 17d to enter
Row third time pinching, after third time pinching, 250g calcium magnesium phosphate is sprayed to water 40kg to plant blade face by every mu；
(6) apply fertilizer：After buddingging, every mu sprays 250g calcium magnesium phosphate every time per 9d to plant foliage-spray foliar fertilizer once
To water 40kg, and 180g potassium sulfates are to water 35kg, when it is fickle in love scatter 2/3 when by harvest.
Embodiment three
A kind of cultural method of chrysanthemum, comprises the following steps：
(1), the selection of explant and sterilizing：Stem apex, stem section and lateral bud from chrysanthemum as explant, in 5~August,
The chrysanthemum tender stem young sprout of robust growth is intercepted, the stem section and the lateral bud of health of 3cm after terminal bud, terminal bud is taken, is cleaned with clear water, then
With 80% Ethanol Treatment 35s, 1.5% mercuric chloride treatment 5min, aseptic water washing 5 times is standby, to stem apex and bud, need to be under anatomical lens
Carefully peel off, and the tip of 1.5mm is cut with knife, be uprightly placed on culture medium, stem section is then directly placed on culture medium；
(2), culture medium processed：In parts by weight, its formula is made up of the culture medium following component：2 parts of N15-P15-K17,
1.5 parts of potassium chloride, 1.5 parts of urea, 1.5 parts of calcium nitrate type composite fertilizer N12.2-P0-K0-Ca24.5, EDDHA chelated irons 0.8
Part, each composition of the above halves and is made root media, and a certain amount of basic element of cell division and auxin are added in above-mentioned culture medium,
Differential medium is configured to, the basic element of cell division uses benzylaminopurine, 5mg/L only to be added in differential medium, auxin
Using methyl α-naphthyl acetate, consumption is 0.015mg/L in differential medium, and consumption is 0.3mg/L in root media；
(3), indoor culture explant:Explant is initially positioned on differential medium and is grown, entered after differentiation is sprouted
Row squamous subculture, when further culture makes bud grow to 35mm, is transferred on root media, it is grown up to whole plant, connects
6d after explant is planted, is cultivated under half-light, award the illumination 16h of 2000lx daily later, cultivation temperature is 26 DEG C；
(4), outdoor transplanting：Garden ground requires that the soil is porous, and draining is good, and soil will be made whole good ground through ploughing deeply thin rake
Into high ridge, the culture medium of seedling root is cleaned, is planted on seedbed, starting 2-4d will take cool canopy and spray water in time or spray,
Avoid intense light irradiation and ensure humidity high, illumination progressively can be suitably increased after 2d, water daily 2 times, summer high-temperature waters 3 times,
And weed with a hoe and apply fertilizer in time, ensure seedling healthy growth；
(5) pinching：First time pinching is carried out when height of seedling is to 25cm, second pinching is carried out after 20d, then is spaced 20d to enter
Row third time pinching, after third time pinching, 300g calcium magnesium phosphate is sprayed to water 50kg to plant blade face by every mu；
(6) apply fertilizer：After buddingging, every mu sprays 300g calcium magnesium phosphate every time per 10d to plant foliage-spray foliar fertilizer once
To water 50kg, and 200g potassium sulfates are to water 50kg, when it is fickle in love scatter 2/3 when by harvest.
Explanation embodiments of the present invention are the foregoing is only, is not intended to limit the invention, for the technology of this area
For personnel, all any modification, equivalent substitution and improvements within the spirit and principles in the present invention, made etc. should be included in
Within protection scope of the present invention.
Claims (1)
1. a kind of cultural method of chrysanthemum, it is characterised in that comprise the following steps：
(1), the selection of explant and sterilizing：Stem apex, stem section and lateral bud from chrysanthemum, in 5~August, are intercepted as explant
The chrysanthemum tender stem young sprout of robust growth, takes the stem section and the lateral bud of health of 3cm after terminal bud, terminal bud, is cleaned with clear water, Ran Houyong
60%-80% 25-35s of Ethanol Treatment, 0.5%-1.5% mercuric chloride processes 3-5min, and aseptic water washing 3-5 times is standby, right
Stem apex and bud, need to carefully be peeled off under anatomical lens, and the tip of 0.5~1.5mm is cut with knife, be uprightly placed on culture medium, stem
Duan Ze is directly placed on culture medium；
(2), culture medium processed：In parts by weight, its formula is made up of the culture medium following component：N15-P15-K171-2 parts, chlorine
Change 1-1.5 parts of potassium, urea 1-1.5 parts, 1-1.5 parts of calcium nitrate type composite fertilizer N12.2-P0-K0-Ca24.5, EDDHA chelated irons
0.5-0.8 parts, each composition of the above halves and is made root media, added in above-mentioned culture medium a certain amount of basic element of cell division and
Auxin, is configured to differential medium, and the basic element of cell division uses benzylaminopurine, 1-5mg/L only to add in differential medium
Enter, auxin uses methyl α-naphthyl acetate, consumption is 0.005-0.015mg/L in differential medium, and consumption is in root media
0.1-0.3mg/L；
(3), indoor culture explant:By explant be initially positioned on differential medium grow, carried out after differentiation is sprouted after
It is commissioned to train foster, when further culture makes bud grow to 25-35mm, is transferred on root media, it is grown up to whole plant, is inoculated with
4-6d after explant, cultivates under half-light, awards the 12~16h of illumination of 1400~2000lx daily later, and cultivation temperature is 23-
26℃；
(4), outdoor transplanting：Garden ground requires that the soil is porous, and draining is good, and whole good ground will be made height by soil through ploughing deeply thin rake
Furrow, the culture medium of seedling root is cleaned, and is planted on seedbed, and starting 2-4d will take cool canopy and spray water in time or spray, it is to avoid
Intense light irradiation simultaneously ensures humidity high, and illumination progressively can be suitably increased after 1~2d, waters daily 1~2 time, summer high-temperature waters 2~
3 times, and weed with a hoe in time and apply fertilizer, ensure seedling healthy growth；
(5) pinching：First time pinching is carried out when height of seedling is to 25cm, second pinching is carried out after 15~20d, then it is spaced 15~
20d carries out third time pinching, after third time pinching, to plant blade face by every mu spray 200~300g calcium magnesium phosphate to water 30~
50kg；
(6) apply fertilizer：After buddingging, to plant foliage-spray foliar fertilizer once, every mu sprays 200~300g calcium and magnesiums to every 8~10d every time
Phosphate fertilizer is to 30~50kg of water, and 150~200g potassium sulfates are to 20~50kg of water, when it is fickle in love scatter 2/3 when by harvest.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611018437.3A CN106688884A (en) | 2016-11-18 | 2016-11-18 | Chrysanthemum cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611018437.3A CN106688884A (en) | 2016-11-18 | 2016-11-18 | Chrysanthemum cultivation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106688884A true CN106688884A (en) | 2017-05-24 |
Family
ID=58940586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611018437.3A Pending CN106688884A (en) | 2016-11-18 | 2016-11-18 | Chrysanthemum cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106688884A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651717A (en) * | 2019-11-20 | 2020-01-07 | 四川云辰园林科技有限公司 | Chrysanthemum seedling tissue culture detoxification and rapid propagation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4038778A (en) * | 1976-09-27 | 1977-08-02 | Gte Laboratories Incorporated | Tissue culture technique for asexual plant reproduction and a medium for use therewith |
| CN102301952A (en) * | 2011-07-19 | 2012-01-04 | 北京林业大学 | Method for breeding chamomile |
| CN103250638A (en) * | 2013-04-17 | 2013-08-21 | 池州市天健生态农业发展有限公司 | Chrysanthemum tissue culture method |
| CN103340152A (en) * | 2013-06-28 | 2013-10-09 | 新疆凤凰远山农业发展有限公司 | Method for screening, detoxifying and rapidly propagating chrysanthemum |
| CN105052746A (en) * | 2015-08-24 | 2015-11-18 | 华南农业大学 | Echinacea adventitious bud regeneration culture method |
-
2016
- 2016-11-18 CN CN201611018437.3A patent/CN106688884A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4038778A (en) * | 1976-09-27 | 1977-08-02 | Gte Laboratories Incorporated | Tissue culture technique for asexual plant reproduction and a medium for use therewith |
| CN102301952A (en) * | 2011-07-19 | 2012-01-04 | 北京林业大学 | Method for breeding chamomile |
| CN103250638A (en) * | 2013-04-17 | 2013-08-21 | 池州市天健生态农业发展有限公司 | Chrysanthemum tissue culture method |
| CN103340152A (en) * | 2013-06-28 | 2013-10-09 | 新疆凤凰远山农业发展有限公司 | Method for screening, detoxifying and rapidly propagating chrysanthemum |
| CN105052746A (en) * | 2015-08-24 | 2015-11-18 | 华南农业大学 | Echinacea adventitious bud regeneration culture method |
Non-Patent Citations (2)
| Title |
|---|
| 张平: "非洲菊组织培养研究进展", 《宁德师专学报(自然科学版)》 * |
| 张燕等: "菊花组织培养技术研究进展", 《廊坊师范学院学报(自然科学版)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651717A (en) * | 2019-11-20 | 2020-01-07 | 四川云辰园林科技有限公司 | Chrysanthemum seedling tissue culture detoxification and rapid propagation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103004449B (en) | Cultivation and management method of organic cotton | |
| CN104041272A (en) | Pollution-free greenhouse strawberry cultivation method | |
| CN108967070B (en) | Method for planting passion fruit by soil renovation in next year | |
| CN107333554A (en) | A kind of cultural method of chrysanthemum high yield | |
| CN104770198A (en) | Lily planting method | |
| CN104855127A (en) | Chrysanthemum cultivation method | |
| CN107371721A (en) | A kind of cultural method of dragon fruit high-yield and high-efficiency | |
| CN105684844A (en) | Dendrobium officinale planting method | |
| CN106359087B (en) | A kind of lucid asparagus tissue-culturing rapid propagation method for culturing seedlings | |
| CN105918051A (en) | Greenhouse cultivation method for nectarines | |
| CN106986691A (en) | A kind of cultural method of spun gold emperor chrysanthemum | |
| CN107750855A (en) | A kind of pest control method during strawberry cultivating | |
| CN104770181A (en) | Strawberry planting method | |
| CN105027888A (en) | Planting method of decaisne fargesii | |
| CN108293769A (en) | A kind of Protectorate cultivation method of green precious small pumpkin | |
| CN112106608A (en) | Strawberry seedling breeding method in northwest cold arid region | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN104335873A (en) | Method for cultivating Jingmian persimmon trees | |
| CN107466656B (en) | Comprehensive control method for soil-borne diseases for pepper cultivation | |
| CN105075619A (en) | Cultivation and seed selection method for lily | |
| CN106688884A (en) | Chrysanthemum cultivation method | |
| CN104054569A (en) | Selective breeding method production process of wild dendrobium officinale | |
| CN107493930A (en) | A kind of autumn culture method of green precious small pumpkin | |
| CN107517715A (en) | A kind of implantation methods of the stem of noble dendrobium | |
| CN108293770A (en) | A kind of open field culture method of green precious small pumpkin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |