CN1653879A - A flower cutting and seed planting rapid breeding method for flameray gerbera - Google Patents
A flower cutting and seed planting rapid breeding method for flameray gerbera Download PDFInfo
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- CN1653879A CN1653879A CN 200410052481 CN200410052481A CN1653879A CN 1653879 A CN1653879 A CN 1653879A CN 200410052481 CN200410052481 CN 200410052481 CN 200410052481 A CN200410052481 A CN 200410052481A CN 1653879 A CN1653879 A CN 1653879A
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Abstract
The present invention discloses the fast germchit propagating method for flameray gerbera cut flower, and aims at providing one kind of fast propagating method of flameray gerbera germchit with less germchit variability, consistent characteristic and robust growth. The method includes cutting the floral receptacle of flameray gerbera bud as the explant, cutting the floral receptacle into small blocks, culturing in inducing culture medium at proper lighting and temperature to grow indefinite bud, culturing the indefinite bud in proliferating culture medium at proper lighting and temperature to grow bush bud, cutting the bush bud into single seedlings, culturing the seedlings in rooting culture medium at proper lighting and temperature to induce rooting, and finally transplanting the rooted seedling in seedling bed. The present invention is applied in propagation of flower.
Description
Technical field
The present invention relates to a kind of cut gerbera flower quick reproduction technique method.
Background technology
Flameray gerbera (Gebera jamesonii Bolus) has another name called African daisy, heronsbill, is perennial perennial root herbaceous plant, is fast-selling both at home and abroad fresh flower and potted plant famous-brand and high-quality flowers.The flameray gerbera flower is very large, it is gorgeous to can be used for rich color, can be used as the ideal material of gift bouquet, the gaily decorated basket and artistic flower arrangement, also can be used as the important ornaments of grade places.The propagation method that flameray gerbera is traditional is sowing or division propagation.Because seed longevity is short, germination rate is low, the breeding aberration rate is very high, so seed propagation is difficult to obtain the seedling of proterties unanimity, can not be used for large-scale production; Division propagation then exists reproduction coefficient low, degenerates easily and propagates disease, also is unsuitable for modern large-scale production.Therefore, the defective that exists of the propagation method of existing flameray gerbera seedling is: reproduction speed is slow, seedling aberration rate height, inconsistent, the Failure to thrive of proterties.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, provides that a kind of reproduction speed is fast, the seedling aberration rate is low, proterties is consistent, the cut gerbera flower quick reproduction technique method of robust growth.
The technical solution adopted in the present invention is: cut gerbera flower quick reproduction technique method of the present invention mainly may further comprise the steps:
(1) getting flameray gerbera bennet length is the bud of 1～3cm, takes out after described bud was sterilized 12 minutes in 0.1% mercuric chloride solution, uses aseptic water washing 7～8 times;
(2) cut the holder position of the described bud after disinfecting as growing body outward;
(3) the described outer body branch of growing is cut to 3～5mm fritter and places on the inducing culture, in intensity of illumination is that 1000Lux, temperature are to cultivate under 22～28 ℃ the condition, induce described outer growing to produce indefinite bud on the body, described inducing culture is the MS medium that is added with AKT0～2.0PPM, NAA0～0.2PPM, BA0～1.0PPM, IAA0～0.5PPM;
When (4) described indefinite bud to be induced grows to 3～4 leaves, leaves and is about 3～4cm size, downcut described indefinite bud and place on the proliferated culture medium, in intensity of illumination is that 1500～2000Lux, temperature are to cultivate under 22～28 ℃ the condition, described indefinite bud forms the clump bud through 20～25 days enrichment culture, and described proliferated culture medium is the MS medium that is added with BA0.2～2.0PPM, NAA0.2PPM;
(5) described clump bud is cut into single plant sprout and places on the root media, in intensity of illumination is that 1500～2000Lux, temperature are to carry out root induction under 22～28 ℃ the condition, described single plant sprout is formed with offspring through a month culture of rootage, and described root media is the 1/2MS medium that is added with IBA0.5PPM;
(6) there is offspring to be transplanted to the seedbed with described.
The invention has the beneficial effects as follows: because the present invention cuts the holder of flameray gerbera bud as growing body outward, earlier it is cut into small pieces to be placed on the inducing culture and under suitable illumination and temperature, induces indefinite bud, again indefinite bud is downcut and place on the proliferated culture medium enrichment culture Cheng Congya under suitable illumination and temperature, again the clump bud being cut into single plant sprout and placing on the root media induces it to take root under suitable illumination and temperature, transplantation of seedlings will take root at last to the seedbed, each breeding cultivation stage all carries out under suitable condition, and described reproduction speed is fast and seedling aberration rate that breed is low, the proterties unanimity, robust growth.
Embodiment
Embodiment one:
Cut gerbera flower quick reproduction technique method of the present invention mainly may further comprise the steps:
(1) gets flameray gerbera bennet length and be the bud of 1cm, take out after described bud was sterilized 12 minutes in 0.1% mercuric chloride solution, use aseptic water washing 7 times;
(2) cut the holder position of the described bud after disinfecting as growing body outward;
(3) the described outer body branch of growing being cut to the 3mm fritter and placing on the inducing culture, is that 1000Lux, temperature are to cultivate under 22 ℃ the condition in intensity of illumination, induces described outer growing to produce indefinite bud on the body.Described inducing culture is the MS medium;
When (4) described indefinite bud to be induced grows to 3 leaves, leaves and is about the 3cm size, downcut described indefinite bud and place on the proliferated culture medium, in intensity of illumination is that 1500Lux, temperature are to cultivate under 22 ℃ the condition, described indefinite bud forms the clump bud through 20 days enrichment culture, and described proliferated culture medium is the MS medium that is added with BA0.2PPM, NAA0.2PPM;
(5) described clump bud is cut into single plant sprout and places on the root media, in intensity of illumination is that 1500Lux, temperature are to carry out root induction under 22 ℃ the condition, described single plant sprout is formed with offspring through a month culture of rootage, and described root media is the 1/2MS medium that is added with IBA0.5PPM;
(6) there is offspring to be transplanted to the seedbed with described.
Each breeding cultivation stage of flameray gerbera of the present invention all carries out under suitable condition, and described reproduction speed is fast and seedling aberration rate that breed is low, proterties is consistent, robust growth.
Embodiment two:
Cut gerbera flower quick reproduction technique method of the present invention mainly may further comprise the steps:
(1) gets flameray gerbera bennet length and be the bud of 3cm, take out after described bud was sterilized 12 minutes in 0.1% mercuric chloride solution, use aseptic water washing 8 times;
(2) cut the holder position of the described bud after disinfecting as growing body outward;
(3) the described outer body branch of growing is cut to the 5mm fritter and places on the inducing culture, in intensity of illumination is that 1000Lux, temperature are to cultivate under 28 ℃ the condition, induce described outer growing to produce indefinite bud on the body, described inducing culture is the MS medium that is added with AKT2.0PPM, NAA0.2PPM, BA1.0PPM, IAA0.5PPM;
When (4) described indefinite bud to be induced grows to 4 leaves, leaves and is about the 4cm size, downcut described indefinite bud and place on the proliferated culture medium of the BA that is added with variable concentrations, in intensity of illumination is that 2000Lux, temperature are to cultivate under 28 ℃ the condition, described indefinite bud forms the clump bud through 25 days enrichment culture, and described proliferated culture medium is the MS medium that is added with BA2.0PPM, NAA0.2PPM;
(5) described clump bud is cut into single plant sprout and places on the root media, in intensity of illumination is that 2000Lux, temperature are to carry out root induction under 28 ℃ the condition, described single plant sprout is formed with offspring through a month culture of rootage, and described root media is the 1/2MS medium that is added with IBA0.5PPM;
(6) there is offspring to be transplanted to the seedbed with described.
All the other features are with embodiment one.
Embodiment three:
Cut gerbera flower quick reproduction technique method of the present invention mainly may further comprise the steps:
(1) gets flameray gerbera bennet length and be the bud of 2cm, take out after described bud was sterilized 12 minutes in 0.1% mercuric chloride solution, use aseptic water washing 8 times;
(2) cut the holder position of the described bud after disinfecting as growing body outward;
(3) the described outer body branch of growing is cut to the 4mm fritter and places on the inducing culture, in intensity of illumination is that 1000Lux, temperature are to cultivate under 25 ℃ the condition, induce described outer growing to produce indefinite bud on the body, described inducing culture is the MS medium that is added with AKT1.0PPM, NAA0.1PPM, BA0.5PPM, IAA0.3PPM;
When (4) described indefinite bud to be induced grows to 4 leaves, leaves and is about the 4cm size, downcut described indefinite bud and place on the proliferated culture medium of the BA that is added with variable concentrations, in intensity of illumination is that 1800Lux, temperature are to cultivate under 25 ℃ the condition, described indefinite bud forms the clump bud through 23 days enrichment culture, and described proliferated culture medium is the MS medium that is added with BA0.6PPM, NAA0.2PPM;
(5) described clump bud is cut into single plant sprout and places on the root media, in intensity of illumination is that 1800Lux, temperature are to carry out root induction under 25 ℃ the condition, described single plant sprout is formed with offspring through a month culture of rootage, and described root media is the 1/2MS medium that is added with IBA0.5PPM;
(6) there is offspring to be transplanted to the seedbed with described.
All the other features are with embodiment one.
Claims (1)
1, a kind of cut gerbera flower quick reproduction technique method, it is characterized in that: it mainly may further comprise the steps:
(1) getting flameray gerbera bennet length is the bud of 1～3cm, takes out after described bud was sterilized 12 minutes in 0.1% mercuric chloride solution, uses aseptic water washing 7～8 times;
(2) cut the holder position of the described bud after disinfecting as growing body outward;
(3) the described outer body branch of growing is cut to 3～5mm fritter and places on the inducing culture, in intensity of illumination is that 1000Lux, temperature are to cultivate under 22～28 ℃ the condition, induce described outer growing to produce indefinite bud on the body, described inducing culture is the MS medium that is added with AKT 0～2.0PPM, NAA 0～0.2PPM, BA 0～1.0 PPM, IAA0～0.5PPM;
When (4) described indefinite bud to be induced grows to 3～4 leaves, leaves and is about 3～4cm size, downcut described indefinite bud and place on the proliferated culture medium, in intensity of illumination is that 1500～2000Lux, temperature are to cultivate under 22～28 ℃ the condition, described indefinite bud forms the clump bud through 20～25 days enrichment culture, and described proliferated culture medium is the MS medium that is added with BA0.2～2.0PPM, NAA0.2PPM;
(5) described clump bud is cut into single plant sprout and places on the root media, in intensity of illumination is that 1500～2000Lux, temperature are to carry out root induction under 22～28 ℃ the condition, described single plant sprout is formed with offspring through a month culture of rootage, and described root media is the 1/2MS medium that is added with IBA0.5PPM;
(6) there is offspring to be transplanted to the seedbed with described.
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| CN 200410052481 CN1653879A (en) | 2004-12-02 | 2004-12-02 | A flower cutting and seed planting rapid breeding method for flameray gerbera |
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| CN 200410052481 CN1653879A (en) | 2004-12-02 | 2004-12-02 | A flower cutting and seed planting rapid breeding method for flameray gerbera |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496496B (en) * | 2008-02-02 | 2012-02-08 | 山东省果树研究所 | Tissue culture and rapid propagation method for Gerbera jamesonii Bolus |
| CN103250638A (en) * | 2013-04-17 | 2013-08-21 | 池州市天健生态农业发展有限公司 | Chrysanthemum tissue culture method |
| CN104396744A (en) * | 2014-11-04 | 2015-03-11 | 盐城呈祥园艺育苗有限公司 | African daisy tissue culture seedling growing method |
| CN109463274A (en) * | 2019-01-04 | 2019-03-15 | 玉溪云星生物科技有限公司 | A kind of breeding method of African Chrysanthemum new varieties |
| CN112369328A (en) * | 2020-11-15 | 2021-02-19 | 云南省农业科学院花卉研究所 | Rapid cultivation method of gerbera jamesonii excellent strain |
-
2004
- 2004-12-02 CN CN 200410052481 patent/CN1653879A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496496B (en) * | 2008-02-02 | 2012-02-08 | 山东省果树研究所 | Tissue culture and rapid propagation method for Gerbera jamesonii Bolus |
| CN103250638A (en) * | 2013-04-17 | 2013-08-21 | 池州市天健生态农业发展有限公司 | Chrysanthemum tissue culture method |
| CN103250638B (en) * | 2013-04-17 | 2016-08-31 | 池州市天健生态农业发展有限公司 | A kind of chrysanthemum tissue culture method |
| CN104396744A (en) * | 2014-11-04 | 2015-03-11 | 盐城呈祥园艺育苗有限公司 | African daisy tissue culture seedling growing method |
| CN104396744B (en) * | 2014-11-04 | 2016-08-24 | 盐城呈祥园艺育苗有限公司 | African Chrysanthemum tissue culture method |
| CN109463274A (en) * | 2019-01-04 | 2019-03-15 | 玉溪云星生物科技有限公司 | A kind of breeding method of African Chrysanthemum new varieties |
| CN112369328A (en) * | 2020-11-15 | 2021-02-19 | 云南省农业科学院花卉研究所 | Rapid cultivation method of gerbera jamesonii excellent strain |
| CN112369328B (en) * | 2020-11-15 | 2022-08-12 | 云南省农业科学院花卉研究所 | Rapid cultivation method of gerbera jamesonii excellent strain |
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