CN107858293A - A kind of golden yellow basket bacterium and its application - Google Patents
A kind of golden yellow basket bacterium and its application Download PDFInfo
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- CN107858293A CN107858293A CN201710946541.7A CN201710946541A CN107858293A CN 107858293 A CN107858293 A CN 107858293A CN 201710946541 A CN201710946541 A CN 201710946541A CN 107858293 A CN107858293 A CN 107858293A
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- golden yellow
- basket bacterium
- yellow basket
- jxbr04
- phosphate
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- 241000894006 Bacteria Species 0.000 title claims abstract description 50
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 title claims abstract description 36
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000011574 phosphorus Substances 0.000 claims abstract description 41
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 41
- 230000001580 bacterial effect Effects 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 26
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000011591 potassium Substances 0.000 claims abstract description 23
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 23
- 239000002689 soil Substances 0.000 claims abstract description 23
- 241000638881 Talaromyces aurantiacus Species 0.000 claims abstract description 18
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 15
- 230000012010 growth Effects 0.000 claims abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 11
- 235000015097 nutrients Nutrition 0.000 claims abstract description 11
- 239000010452 phosphate Substances 0.000 claims abstract description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 10
- 238000004321 preservation Methods 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 17
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 14
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 14
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 14
- 239000011425 bamboo Substances 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 10
- 239000005955 Ferric phosphate Substances 0.000 claims description 9
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 claims description 9
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 9
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 claims description 9
- 229910000399 iron(III) phosphate Inorganic materials 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 6
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 229940032958 ferric phosphate Drugs 0.000 claims description 5
- WPEXVRDUEAJUGY-UHFFFAOYSA-B hexacalcium;(2,3,4,5,6-pentaphosphonatooxycyclohexyl) phosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])(=O)OC1C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C1OP([O-])([O-])=O WPEXVRDUEAJUGY-UHFFFAOYSA-B 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 2
- 239000008188 pellet Substances 0.000 claims description 2
- 238000004080 punching Methods 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims 1
- 244000046052 Phaseolus vulgaris Species 0.000 claims 1
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 229940009859 aluminum phosphate Drugs 0.000 claims 1
- 239000001506 calcium phosphate Substances 0.000 claims 1
- 235000019731 tricalcium phosphate Nutrition 0.000 claims 1
- 229940078499 tricalcium phosphate Drugs 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 8
- 238000011161 development Methods 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract 1
- 241001330002 Bambuseae Species 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 11
- 244000005700 microbiome Species 0.000 description 10
- 239000011575 calcium Substances 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 230000003381 solubilizing effect Effects 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- 244000302661 Phyllostachys pubescens Species 0.000 description 4
- 235000003570 Phyllostachys pubescens Nutrition 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000486634 Bena Species 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 241000228341 Talaromyces Species 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- PCHPORCSPXIHLZ-UHFFFAOYSA-N diphenhydramine hydrochloride Chemical compound [Cl-].C=1C=CC=CC=1C(OCC[NH+](C)C)C1=CC=CC=C1 PCHPORCSPXIHLZ-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000001038 titanium pigment Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 230000006518 acidic stress Effects 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 229910000323 aluminium silicate Inorganic materials 0.000 description 2
- DLHONNLASJQAHX-UHFFFAOYSA-N aluminum;potassium;oxygen(2-);silicon(4+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Al+3].[Si+4].[Si+4].[Si+4].[K+] DLHONNLASJQAHX-UHFFFAOYSA-N 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000002949 phytic acid Nutrition 0.000 description 2
- 238000004382 potting Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 241001330024 Bambusoideae Species 0.000 description 1
- 208000019025 Hypokalemia Diseases 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241000371966 Penicillus <bivalve> Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000745988 Phyllostachys Species 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 239000010433 feldspar Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002367 phosphate rock Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 208000007645 potassium deficiency Diseases 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a kind of golden yellow basket bacterium, its Classification And Nomenclature is golden yellow basket bacterium（Talaromyces aurantiacus）, bacterial strain number is JXBR04, is deposited in China typical culture collection center, and deposit number is CCTCC NO：M 2017327, preservation date are on June 13rd, 2017.The present invention not only has efficient phosphorus decomposing function, the ability also with preferable potassium decomposing and producing IAA from the golden yellow basket bacterium of mao bamboon rhizosphere soil screening.Under conditions of liquid shakes training, there is stronger solvability to insoluble phosphate, promote slightly solubility phosphorus element to be converted into soluble nutrient；The invention also discloses application of the golden yellow basket bacterium in mao bamboon growth promotion, liquid bacterial agent made of the golden yellow basket bacterium of the present invention has significant growth promoting function to mao bamboon ground diameter and height of seedling, therefore, the present invention provides excellent strain resource for development environment friendly mao bamboon biology dedicated fertilizer.
Description
Technical field
The present invention relates to microorganism and biological fertilizer field, is related to a kind of golden yellow basket bacterium and its application.
Background technology
Mao bamboon (Phyllostachys edulis) is the commodity trees of grass family Bambusoideae.According to incompletely statistics, China
Existing mao bamboo woods account for the 80% of 70% and global mao bamboon area of national bamboo grove area more than 3,800,000 ha.In being permitted for south China
More mountain areas, the main economic seed of forest that mao bamboon has increased income as agricultural industry restructuring, forestry synergy, Lin Min, but current hair
For bamboo management mostly based on extensive style, long-term unreasonable operation causes that bamboo stand soil fertility is weak, nutrient supply is insufficient, special
It is not the shortage of phosphorus element, this, which turns into during bamboo resource is cultivated, faces one of subject matter；Mao bamboon people can be remarkably promoted using chemical fertilizer
The growth of work phosphorus, however, increasing sharply with global energy crisis and fertilizer application amount, its negative effect is increasingly obvious.Cause
This, seeks new fertilizer, especially biomass fertilizers, is received much attention with the research of replacement or part replacing fertilizer.Mao bamboo woods are past
Toward the Red Soils In A Hill Region area for being distributed in scarce phosphorus, nearest research is it has been shown that phosphorus has turned into the primary factor of limitation Productivity of Phyllostachys.Phosphorus
It is one of nutrient necessary to Bamboo Growth development and biomass accumulation.More than 95% phosphorus is difficult direct by plant in red soil
Absorb, and global environmental change, aggravate the unavailability of phosphorus.Soil phosphorus, which lacks, causes bamboo wood, bamboo shoot output year by year
On a declining curve, the going concern that serious threat mao bamboo woods utilizes.In addition, potassium is one of three elements of plant nutrient, in sugar
Played an important role in the physiology courses such as metabolism, protein synthesis and enhancing plant resistance to environment stress.However, China there are about 60% arable land
Potassium deficiency, 95% potassium is mineral Potassium forms in soil, is existed in potassium feldspar and mica, the potassium for being available for plant absorption to utilize does not surpass
The 2% of full potassium is crossed, have impact on the development of agriculture and forestry.Mending potassium currently with Chemical Potassium, not only cost is high, and can cause soil
The environmental problems such as structure is destroyed, the content of organic matter declines.
Rhizosphere microorganism is the crucial participant and regulation and control person of rhizosphere process.Rhizosphere soil microorganism is supported by changing soil
Point validity and directly or indirectly influence structure of plant community and productivity with the mode of root system of plant symbiosis.Early in 20 generation
Record just, people begin to pay close attention to the relation between microorganism and soil phosphorus.Sackett (1908) has found some originally not first
Molten phosphate and natural rock phosphate in powder can be dissolved utilization by some bacteriums.Hereafter, using rhizosphere microorganism to insoluble phosphorus
Dissolution come promote plant growth and improve Phosphorus Nutrition in Plants situation research carried out extensively.
Research shows, exist in edaphon it is many have by the phosphorus of slightly solubility, potassium be converted into plant can utilize phosphorus,
The microorganism of potassium ability, referred to as phosphate solubilizing microorganism and silicate-dissolving microbe.Phosphate solubilizing microorganism is in P in soil biochemical cycles mistake
Played an important role in journey, slightly solubility Phos and organophosphor in soil can be converted into by they by dissolving and mineralization
Available P, absorb for plant (Whitelaw et al., 1999).According to incompletely statistics, the whole world is screened altogether at present
Go out 30 category, 89 kinds of phosphate solubilizing microorganisms, including phosphorus decomposing fungi, phosphate-solubilizing bacteria and phosphorus decomposing actinomyces etc..Phosphorus decomposing fungi
(Solubilizing phosphorus fungus) although on value volume and range of product be less than phosphate-solubilizing bacteria, there are some researches show
The phosphate solubilization of phosphorus decomposing fungi is typically better than bacterium, sometimes even tens times of bacterium, and inhereditary feature is more stable.
Potassium solubilizing bacteria can the insoluble aluminosilicate inorganic mineral matter such as decomposing of potassium feldspar, apatite, promote the potassium of slightly solubility, phosphorus, silicon,
The nutrient elements such as magnesium change into soluble nutrient, increase the content of available nutrient in soil, promote growth and development of plants, improve production
Amount.Therefore, the important microorganism of one kind during phosphorus decomposing, potassium decomposing fungi are circulated as soil phophorus, the phosphorus of mao bamboon can be improved, potassium supplies
Should, mao bamboon faster, is preferably grown.At present, there is the sieve of the function stem of dissolving phosphor and dissolving potassium and producing IAA about mao bamboon rhizosphere
Choosing and application study have no report.
The content of the invention
For overcome the deficiencies in the prior art, the technical problems to be solved by the invention are to provide a kind of promotion Bamboo Growth
And the golden Tarlaromyces flavus of the ability to the solvability and producing IAA of insoluble phosphate in soil and alumino-silicate.
The technical problem of the invention also to be solved is to provide above-mentioned golden yellow basket bacterium answering in Bamboo Growth is promoted
With.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
From the 5 years setation bamboo rhizosphere soils in Jiangxi Province Yichun City Yifeng County huge port forest farm, screening obtains plant height effect solution
The golden yellow basket bacterium (Talaromyces aurantiacus) of phosphorus, bacterial strain number is JXBR04.It has been preserved in Chinese Typical Representative culture guarantor
Tibetan center (CCTCC), address：Wuhan University of Wuhan City of Hubei China province, postcode：430072, deposit number is：CCTCC NO：M
2017327, preservation date is on June 13rd, 2017.
Golden yellow basket bacterium (Talaromyces aurantiacus) JXBR04 main biological properties：In Czapek
Bacterium colony circle, bacterium colony front yellow on Yeast Extract Agar culture mediums (CYA) flat board；Conidiophore penicillus, spore
Bulbec shape, it is unicellular.
Golden yellow basket bacterium (Talaromyces aurantiacus) IST, BenA and CaM sequence is shown in SEQ ID No：1 institute
Show.Surveyed ITS, BenA and CaM sequence is compared with the sequence in GeneBank databases.As a result show,
JXBR04 bacterial strains and Talaromyces (Talaromyces) homology are very high, with Talaromyces aurantiacus, phase knowledge and magnanimity
Reach 100%.Combining form and ITS, BenA and CaM sequence analysis, it is accredited as golden yellow basket bacterium (Talaromyces
aurantiacus)。
Slightly solubility Phos in soil can be changed into titanium pigment supply mao bamboon and absorb and utilize by JXBR04 bacterial strains, and then
Promote the growth of mao bamboon.
Present invention also offers a kind of preparation method of liquid bacterial agent, concrete operations are as follows：
1) golden yellow basket bacterium (Talaromyces aurantiacus) the JXBR04 inoculations to PDA solids are trained
Support on base,
2) treat that mycelia covers with flat board, golden yellow basket bacterium (Talaromyces will be carried with aseptic card punch
Aurantiacus) the flat board punching of JXBR04 mycelia,
3) again with choose pin bacteria cake and be transferred in the 250ml triangular flasks equipped with 150ml PDA liquid mediums expand it is numerous, every bottle
10 pieces of inoculation, 25 DEG C, 6~8d is cultivated under the conditions of 121r/min, treats that mycelium pellet covers with fluid nutrient medium i.e. liquid bacterial agent.
Further, the diameter d of the aseptic card punch is 7mm.
Further, in step 3), before expansion is numerous, PDA liquid medium is through 1.01 × 106Pa, 121 DEG C of sterilizing 20min
Processing, keep germ-free condition.
Beneficial effect：Compared with prior art, the golden yellow basket bacterium (Talaromyces aurantiacus) of the present invention is not only
With efficient phosphate-solubilizing function, the ability also with preferable potassium decomposing and producing IAA.Indoors under liquid culture condi, to indissoluble
Acid phosphate tricalcium phosphate (Ca3(PO4)2), ferric phosphate (FePO4), calcium monohydrogen phosphate (CaHPO4), aluminum phosphate (AlPO4) and phytic acid
Calcium (organic phytate) has stronger dissolution,；The golden yellow basket bacterium of mao bamboon rhizosphere high-efficient phosphate-solubilizing of the present invention is made
Liquid bacterial agent is inoculated with annual Phyllostachys Pubescens Seedlings.As a result showing, the microbial inoculum can be obviously promoted growing for mao bamboon, therefore, this
Invent and provide excellent strain resource to develop mao bamboon microbial fertilizer special in the future.
Brief description of the drawings
Fig. 1 be colonial morphologies of the golden yellow basket bacterium JXBR04 on CYA culture mediums and on NBRIP culture mediums it is caused molten
Phosphorus circle, A figures：Golden yellow basket bacterium cultivates the colonial morphology of 7 days on CYA culture mediums；B schemes：Golden yellow basket bacterium is in NBRIP culture mediums
It is upper to cultivate Soluble phosphorus circle caused by 7 days.
Fig. 2 is the conidiophore and conidium of golden yellow basket bacterium, is opened from left, center, right three in figure it is observed that being in sweep
The conidium stalk of broom shape and the conidium form of ellipse.
Fig. 3 is bacterial strain JXBR04 CaM gene order phylogenetic trees.
Fig. 4 be bacterial strain JXBR04 bacterial strains under condition of different pH to 5 kinds to insoluble phosphate tricalcium phosphate (Ca3
(PO4)2), ferric phosphate (FePO4), calcium monohydrogen phosphate (CaHPO4), aluminum phosphate (AlPO4) and phytic acid calcium (organic phytate)
Solvability.
Fig. 5 is bacterial strain JXBR04 ability of dissolving potassium.
Fig. 6 is bacterial strain JXBR04 producing IAA abilities.
Embodiment
According to following embodiments, those skilled in the art can be made to more fully understand the present invention.Described by embodiment
The present invention is merely to illustrate, without should be also without limitation on the present invention described in detail in claims.
Embodiment 1：JXBR04 bacterial strains growth test under acid stress
By the bacterial strain JXBR04 of activation inoculation PDA liquid medium, (formula of PDA liquid medium is：Potato 200g,
Glucose 20g, distilled water 1000mL) in, 25 DEG C, 120r/min shaken cultivations 5d is as seed liquor, by PDA liquid medium
PH is arranged to 1.5,2.5,3.5,4.5,5.5,6.5 6 gradients, is sub-packed in 250ml triangular flasks, liquid amount 100mL.With
Afterwards, seed liquor is inoculated into PDA liquid medium respectively by 1% (v/v) inoculum concentration, respectively 3 repetitions of processing, 25 DEG C, 120r/
Min shaken cultivations 8d.48h is dried at 65 DEG C after mycelia filtering, is weighed.The results are shown in Table 1, bacterial strain JXBR04 pH 1.5,
2.5th, preferable, hypha biomass and indifference, and zymotic fluid pH models are grown under 3.5,4.5,5.5 and 6.5 different acidic conditions
Enclose for 3.40~3.95.Indicated above, bacterial strain JXBR04 can adjust itself suitable growth acid ring by secreting acid
Border, and then different sour environments is adapted to, possess the ability grown in different acid soil environments.
Influences of the 1 different pH of table to JXBR04 hypha biomass and zymotic fluid pH
Note：P ＜ 0.05, same column, which is not gone together, small represents difference with alphabetical differ
Embodiment 2：Phosphorus decomposing experiments of the bacterial strain JXBR04 under acid stress.
Phosphorus decomposing culture medium A：Glucose 10g, Ca3(PO4)25g, MgCl25g, KCl 0.2g, MgSO4.7H2O 0.25g,
(NH4)28O40.1g, distilled water 1000mL.
Phosphorus decomposing culture medium B：With aluminum phosphate (AlPO4) instead of the Ca in phosphorus decomposing culture medium A3(PO4)3, other compositions and content
It is identical.
Phosphorus decomposing culture medium C：With ferric phosphate (FePO4) instead of the Ca in phosphorus decomposing culture medium A3(PO4)3, other compositions and content
It is identical.
Phosphorus decomposing culture medium D：With calcium monohydrogen phosphate (CaHPO4) instead of the Ca in phosphorus decomposing culture medium A3(PO4)3, other compositions and
Content is identical.
Phosphorus decomposing culture medium E：With phytic acid calcium (C6H6Ca6O24P6) instead of the Ca in phosphorus decomposing culture medium A3(PO4)3, other compositions
It is identical with content.
By the JXBR04 inoculations PDA culture medium of activation, (formula of PDA culture medium is potato 200g, glucose
20g, agar 18g, distilled water 1000mL) in, 25 DEG C, seed liquor is pressed 1% by 120r/min shaken cultivations 5d as seed liquor
(v/v) inoculum concentration be inoculated into respectively be adjusted to respectively equipped with 50mL and pH 1.5,2.5,3.5,4.5,5.5,6.5 phosphorus decomposing culture medium
A, in B, C, D and E 100mL triangular flasks, using the phosphorus decomposing culture medium for connecing same volume blank seed liquor as control (CK), each locate
Three repetitions are managed, 25 DEG C, after 121r/min cultivates 8d, 4 DEG C of zymotic fluid, 10000r/min centrifuges 10min, and molybdenum antimony resistance colorimetric method is surveyed
Determine titanium pigment content in zymotic fluid,
As a result Fig. 4 is seen.As can be drawn from Figure 4, respectively with insoluble phosphate tricalcium phosphate (Ca3(PO4)2), ferric phosphate
(FePO4), calcium monohydrogen phosphate (CaHPO4), aluminum phosphate (AlPO4) and phytic acid calcium (C6H6Ca6O24P6) it is unique phosphorus source, be in pH
1.5th, in 2.5,3.5,4.5,5.5,6.5 different sour environments, after cultivating 8d, in different sour environments, JXBR04 fermentations
Titanium pigment content is all remarkably higher than CK in liquid.Indicated above, bacterial strain JXBR04 is in different acidic conditions to five kinds of insoluble phosphorus
Hydrochlorate is respectively provided with stronger solvability.
Embodiment 3：JXBR04 bacterial strain ability of dissolving potassium determination tests.
By the access of JXBR04 bacterial strains, equipped with 50mL potassium decomposings culture medium, (formula of potassium decomposing culture medium is：Feldspar in powder 0.1g,
Na2HPO42.0g, FeCl30.005g, MgSO4·7H2O 0.5g, sucrose 5.0g, Ca2CO30.1g, agar 18.0g, distillation
Water 1 000mL, pH 7.0.) 100mL triangular flasks in, 28 DEG C, shaken cultivation 7d obtains zymotic fluid under the conditions of 180r/min, will
The first insoluble substance removed under the conditions of 500r/min, 10min in zymotic fluid of zymotic fluid, then in 10000r/min, 10min
Under the conditions of supernatant is collected by centrifugation, using flame spectrophotometer method determine supernatant in effectively potassium content.As a result such as Fig. 5 institutes
Show, JXBR04 potassium decomposings amount is 15.28mg/L, has preferable ability of dissolving potassium.
Embodiment 4：Bacterial strain JXBR04 produces IAA ability tests.
IAA abilities are produced using Salkowski colorimetric method for determining.Take IAA to mark product, be configured to 0,0.5,2.5,5.0,7.5,
10th, 12.5,15.0,17.5,25.0,50.0mg/L concentration gradient, takes each concentration IAA of 2mL to add the iron chloride colorimetric of equivalent
Liquid (PC color solutions), utilize spectrophotometric determination absorbance under dark insulation 30min, wavelength 530nm in 30 DEG C, draw standard song
Line, obtain equation y=31.868x (R2=0.9947).Bacterial strain JXBR04 after activation is seeded into King culture mediums, and (King is cultivated
Based formulas is：Peptone 2g glycerine 1g, K25O4 0.15g、MgSO4 4.7g、H2O 0.15g, agar 1.5g, H2O 1000ml、pH
7.2) shaking table culture 15d, IAA contents in zymotic fluid are determined by standard curve making method.Result of the test as shown in fig. 6,
JXBR04 has preferably production IAA abilities, and its IAA secretory volume is 21.25mg/L.
Embodiment 5：JXBR04 greenhouse pot cultures are tested：
(1) cultivation of mao bamboon sprout
Mao bamboon seed carries out surface sterilization with 0.5% liquor potassic permanganate immersion 2h, then with after running water flushing 1h, sows
In the sand that sterilizes, it is placed in 25 DEG C of greenhouses and cultivates, sprout section root is carried out when it grows to cotyledon period and is transplanted seedlings.
(2) potting media matrix soil picks up from Agricultural University Of Jiangxi campus Hou Shan, according to soil: sand: vermiculite mass ratio
=2: 1: 1 proportioning, sterilize 2h under the conditions of 165 DEG C, standby after cooling.
(3) preparation of liquid bacterial agent
By the golden yellow basket bacterium strain access PDA solid mediums of preservation, (formula of PDA solid mediums is：Glucose
20g, murphy juice 200g, agar 18g, water 1000mL) in, 6d is cultivated in 25 DEG C, treats that mycelia covers with flat board, aseptically,
Bacteria cake is broken into colony edge with card punch (d=7mm), then is transferred equipped with 250mLPDA fluid nutrient medium (PDA liquid mediums
Formula be：Potato 200g, glucose 20g, distilled water 1000mL) 500mL triangular flasks in expand it is numerous (expand it is numerous before to PDA liquid
Body culture medium is through 1.01 × 106Pa, 121 DEG C sterilizing 20min processing, keep germ-free condition)；10 bacteria cakes of every bottle of picking.Put
In 25 DEG C of cultivation temperature and culture rotating speed 120rmin-1Culture is to mycelium animated period under condition of culture, with the refiner of sterilizing
Crush, be prepared into liquid bacterial agent.
(4) pot experiment of liquid bacterial agent inoculation
Root method transplanting mao bamboon seedling is cut using sprout, microbial inoculum inoculum concentration is 10ml/ strains.Specific method is as follows：Take appropriate nothing
The potting media of bacterium is fitted into flowerpot, and the mao bamboon seedling of cotyledon period is lightly taken out from seedling-growing container, does not injure root system as far as possible,
And rinsed well the sand of seedling root with clear water, transplanting, will into flowerpot after sprout is clipped into a small amount of main root with scalpel
Microbial inoculum is put into around mao bamboon rhizosphere, compacting soil, then is covered substrate soil watering and determined root.To be only inoculated with fluid nutrient medium, not be inoculated with bacterium
Two kinds of processing often handle 10 repetitions as control.25 DEG C of hot-house cultures are placed in, in good time watering, unified management.After mao bamboon inoculation
180d growing states are shown in Table 2, it can be seen that inoculation JXBR04 can remarkably promote the growth of Phyllostachys Pubescens Seedlings.With compareing (CK)
Compare, be inoculated with growth rate of the JXBR04 processing on height of seedling and stem are thick and respectively reached 30.95% and 28.57%, growth-promoting effect
Fruit is notable.
The influence that the bacterial strain JXBR04 of table 2 grows to Phyllostachys Pubescens Seedlings
Note：P ＜ 0.05, same column lowercase of not going together differ and represent difference and show.
Sequence table
<110>Agricultural University Of Jiangxi
<120>A kind of golden yellow basket bacterium and its application
<130> 2017
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 562
<212> DNA
<213>Golden yellow basket bacterium (Talaromyces aurantiacus)
<400> 1
tttccgtagg tgaacctgcg gaaggatcat taccgagtgc gggccctcgc ggcccaacct 60
cccacccgtg tctctctcta cctgttgctt tggcgggccc accggggcca cccggtcgcc 120
gggggacgtc cgtctccggg cccgcgcccg ccgaagcgcc ctgtgaaccc tgatgaagat 180
gggctgtctg agtcgcatga aaattgtcaa aactttcaac aatggatctc ttggttccgg 240
catcgatgaa gaacgcagcg aaatgcgata agtaatgtga attgcagaat tccgtgaatc 300
atcgaatctt tgaacgcaca ttgcgccccc tggcattccg gggggcatgc ctgtccgagc 360
gtcatttctg ccctcaagcg cggcttgtgt gttgggcgtg gtccccccgg ggacctgccc 420
gaaaggcagc ggcgacgtcc gtccggtcct cgagcgtatg gggctctgtc actcgctcgg 480
gacggatcgg cggaggttgg tcaccaccac agttttacca cggttgacct cggatcaggt 540
aggagttacc cgctgaactt aa 562
<210> 2
<211> 415
<212> DNA
<213>Golden yellow basket bacterium (Talaromyces aurantiacus)
<400> 2
tggtgtgtaa aaagactcgg tcaattgtcg ccacaaacaa gctgactttt ccaggcaaat 60
catctctgct gagcacggtc tcgatggctc cggtgtgtaa gtgttgcaaa cgattcgaat 120
gcagttataa tccgacacca tctgatcatc aacagctaca atggctcctc cgacctccag 180
ttggagcgta tgaacgtcta cttcaacgag gtgtgtggaa tcaaccatca gaaaacccat 240
cgaatgcttg gaactcatgt ctcgaatata ggcctccggc aacaagtacg ttccccgtgc 300
cgtcctcgtc gacttggagc ccggtaccat ggatgccgtc cgcgctggtc cctttggtca 360
gctcttccgt cccgacaact ttgttttcgg tcaatccggt gccggtaaca actgg 415
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<211> 662
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<213>Golden yellow basket bacterium (Talaromyces aurantiacus)
<400> 3
aagtctccga gtacaaggag gctttctctc tttttgtaag tctgaaatat ctccctgtcg 60
catactattt tctccttgct tacagaatgt ttttatgaaa taggacaagg atggagatgg 120
tgagtgacga ccgcgaccca ccgacgatgt cgtatcaaaa gcagattcat ctacgattta 180
cgaatgaata ttgatagagt cgaacaggtc aaatcacaac caaggaactt ggcaccgtca 240
tgcgctccct cggccagaac ccctccgaat ccgaattgca ggacatgatc aacgaagttg 300
acgctgacaa caacggcaca attgatttcc ctggtacgat catcacgcct caagctgctt 360
tcaatggaaa aactgaccgc cgcagaattc ttgaccatga tggcccgcaa aatgaaggat 420
accgactccg aggaagagat ccgcgaggcc ttcaaggtgt tcgaccgcga caacaacgga 480
ttcatctccg ccgccgaact gcgtcacgtt atgacctcga ttggcgagaa gctgactgac 540
gatgaggttg acgagatgat ccgcgaggct gaccaggacg gtgatggaag aatcgactgt 600
aagtgcttcc gtgtcctcca cgagataaga gaccggtcta actgttttct tttttagaca 660
ac 662
Claims (10)
1. a kind of golden yellow basket bacterium, its Classification And Nomenclature is golden yellow basket bacterium（Talaromyces aurantiacus）, strain number
For JXBR04, China typical culture collection center is deposited in, deposit number is：CCTCC NO：M 2017327, preservation date
For on June 13rd, 2017.
2. application of the golden yellow basket bacterium in Bamboo Growth is promoted described in claim 1.
3. application according to claim 1, it is characterised in that the golden yellow basket bacterium（Talaromyces aurantiacus）Dissolving applications of the JXBR04 to insoluble phosphate.
4. application according to claim 3, it is characterised in that the golden yellow basket bacterium（Talaromyces aurantiacus）JXBR04 dissolves insoluble phosphate Soluble phosphorus under the gradients of pH 1.5~6.5.
5. application according to claim 3, it is characterised in that the insoluble phosphate include tricalcium phosphate, ferric phosphate,
Aluminum phosphate, calcium monohydrogen phosphate, phytic acid calcium.
6. application according to claim 1, it is characterised in that the golden yellow basket bacterium（Talaromyces aurantiacus）Applications of the JXBR04 in slightly solubility potassium is dissolved.
7. application according to claim 1, it is characterised in that the golden yellow basket bacterium（Talaromyces aurantiacus）JXBR04 has the function of producing IAA.
8. the method for preparing liquid bacterial agent using the golden yellow basket bacterium described in claim 1, it is characterised in that this method includes
Following steps：
1）By the golden yellow basket bacterium（Talaromyces aurantiacus）JXBR04 inoculations are to PDA solid mediums
On,
2）Treat that mycelia covers with flat board, golden yellow basket bacterium will be carried with aseptic card punch（Talaromyces aurantiacus）
The flat board punching of JXBR04 mycelia,
3）Expand numerous, every bottle of inoculation with choosing pin bacteria cake and be transferred in the 250ml triangular flasks equipped with 150ml PDA liquid mediums again
10 pieces, 25 DEG C, 6~8 d are cultivated under the conditions of 121 r/min, treat that mycelium pellet covers with fluid nutrient medium i.e. liquid bacterial agent.
9. the method according to claim 8 for preparing liquid bacterial agent, it is characterised in that step 3）In, before expansion is numerous, PDA liquid
Body culture medium is through 1.01 × 106 Pa, 121 DEG C of sterilizing 20min processing.
10. the method according to claim 8 for preparing liquid bacterial agent, it is characterised in that the PDA solid mediums
Formula is as follows：Glucose 20g, murphy juice 200g, agar 18g, water 1000mL；The formula of the PDA liquid medium is as follows：Soil
The g of fermented bean drink 200, the g of glucose 20, the mL of distilled water 1000.
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