CN107858293B - Talaromyces aurantiaca and application thereof - Google Patents
Talaromyces aurantiaca and application thereof Download PDFInfo
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- CN107858293B CN107858293B CN201710946541.7A CN201710946541A CN107858293B CN 107858293 B CN107858293 B CN 107858293B CN 201710946541 A CN201710946541 A CN 201710946541A CN 107858293 B CN107858293 B CN 107858293B
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a golden basket fungus, which is classified and named as golden basket fungus (a)Talaromyces aurantiacus) The strain number is JXBR04, the strain is preserved in China center for type culture Collection, and the preservation number is CCTCC NO: m2017327, the preservation date is 6 months and 13 days in 2017. The golden basket fungus screened from the rhizosphere soil of the moso bamboo not only has a high-efficiency phosphate-solubilizing function, but also has better potassium-solubilizing and IAA-secreting capabilities. Under the condition of liquid shake culture, the composite fertilizer has stronger dissolving capacity on insoluble phosphate and promotes the conversion of insoluble phosphorus into soluble nutrients; the invention also discloses application of the golden basket fungus in growth promotion of moso bamboos, and the liquid microbial inoculum prepared by the golden basket fungus has remarkable growth promotion effect on the ground diameter and seedling height of the moso bamboos, so that the invention provides excellent strain resources for developing environment-friendly fertilizer special for moso bamboos.
Description
Technical Field
The invention relates to the field of microorganisms and biofertilizers, and relates to a golden basket fungus and application thereof.
Background
Phyllostachys edulis (Phyllostachys edulis) is an economic forest of Bambusoideae of Gramineae. According to incomplete statistics, the existing moso bamboo forest in China exceeds 380 kiloha, and accounts for 70% of the area of the bamboo forest in China and 80% of the area of the moso bamboo forest in the whole world. In many mountainous areas in the south of China, moso bamboos become main economic forest species for structure adjustment of agriculture and forestry industries, efficiency improvement of forestry and income increase of forest people, however, the current moso bamboo forest management is mainly extensive, long-term unreasonable management causes land weakness of the moso bamboo forest and insufficient nutrient supply, especially lack of phosphorus, and the moso bamboo forest land is one of the main problems in moso bamboo resource cultivation; the fertilizer can obviously promote the growth of the artificial phosphorus of the moso bamboo, however, the negative effect is increasingly obvious along with the global energy crisis and the rapid increase of the using amount of the fertilizer. Therefore, research into finding new fertilizers, particularly biomass fertilizers, to replace or partially replace fertilizers has received much attention. Moso bamboo forests are often distributed in hilly red soil areas deficient in phosphorus, and recent studies have shown that phosphorus has become the primary factor limiting the productivity of moso bamboo. Phosphorus is one of the essential nutrient elements for the growth and development of moso bamboos and biomass accumulation. Over 95 percent of phosphorus in the red soil is difficult to be directly absorbed and utilized by plants, and the global environment changes, thus aggravating the unavailability of the phosphorus. The yield of bamboo and bamboo shoots is in a descending trend year by year due to the lack of phosphorus in soil, and the continuous operation and utilization of the moso bamboo forest are seriously threatened. In addition, potassium is one of three elements of plant nutrition, and plays an important role in physiological processes such as sugar metabolism, protein synthesis, plant resistance enhancement and the like. However, about 60% of cultivated land in China is lack of potassium, 95% of potassium in soil is in the form of mineral potassium, potassium exists in potassium feldspar and mica, potassium which can be absorbed and utilized by plants is not more than 2% of total potassium, and the development of agriculture and forestry is influenced. At present, the potassium supplement by using chemical potash fertilizer is not only high in cost, but also causes environmental problems such as soil structure damage, organic matter content reduction and the like.
Rhizosphere microorganisms are key players and regulators of rhizosphere processes. Rhizosphere soil microorganisms directly or indirectly influence plant community structure and productivity by changing the effectiveness of soil nutrients and the way of symbiosis with plant roots. As early as the beginning of the 20 th century, attention has been paid to the relationship between microorganisms and soil phosphorus. Sackett (1908) for the first time discovered that some originally insoluble phosphates and natural phosphate ores could be used by some bacteria. Since then, studies for promoting plant growth and improving plant phosphorus nutrition by utilizing the dissolution of insoluble phosphorus by rhizosphere microorganisms have been widely conducted.
Research shows that a plurality of microorganisms with the capability of converting insoluble phosphorus and potassium into plant available phosphorus and potassium exist in soil microorganisms, and the microorganisms are called as phosphate solubilizing microorganisms and potassium solubilizing microorganisms. Phosphate solubilizing microorganisms play an important role in the biochemical cycle of soil P, and they can convert inorganic phosphorus and organic phosphorus which are difficult to dissolve in soil into available phosphorus through dissolution and mineralization, and the available phosphorus is absorbed and utilized by plants (whitleraw et al, 1999). According to incomplete statistics, at present, 89 phosphate solubilizing microorganisms including phosphate solubilizing fungi, phosphate solubilizing bacteria, phosphate solubilizing actinomycetes and the like are screened out worldwide. Although the number and the types of the phosphate Solubilizing fungi (Solubilizing phosphorus fungi) are less than those of phosphate Solubilizing bacteria, research shows that the phosphate Solubilizing fungi are generally stronger in phosphate Solubilizing capability than the bacteria, sometimes even dozens of times of the bacteria, and the genetic characters are more stable. The potassium-dissolving bacteria can decompose insoluble aluminosilicate inorganic mineral substances such as potassium feldspar, apatite and the like, promote nutrient elements such as insoluble potassium, phosphorus, silicon, magnesium and the like to be converted into soluble nutrients, increase the content of available nutrients in soil, promote plant growth and development and improve yield. Therefore, the phosphate-solubilizing and potassium-solubilizing fungi which are used as an important microorganism in soil phosphorus circulation can improve the supply of phosphorus and potassium of the moso bamboos and enable the moso bamboos to grow faster and better. At present, no report is found about the screening and application research of functional strains with the functions of phosphate and potassium dissolving and IAA secretion of phyllostachys pubescens rhizosphere.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the golden bluefungus for promoting the growth of moso bamboos, and the dissolving capacity and IAA secretion capacity of insoluble phosphate and aluminosilicate in soil.
The invention also aims to solve the technical problem of providing the application of the above golden basket fungus in promoting the growth of moso bamboos.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a strain of efficient phosphate-solubilizing Talaromyces aurantiacas (strain number JXBR 04) is obtained by screening rhizosphere soil of 5-year-old Mao bamboo grown in a large-port forest farm in Yifeng county, Yichun city, Jiangxi province. Has been preserved in China Center for Type Culture Collection (CCTCC) with the address: wuhan university in Wuhan City, Hubei province, China, zip code: 430072, with the preservation number: CCTCC NO: m2017327, the preservation date is 6 months and 13 days in 2017.
The main biological characteristics of the golden basket fungus (Talaromyces aurantiacaus) JXBR04 are as follows: the colony on a Czapek Yeast Extract Agar medium (CYA) plate is round, and the front side of the colony is yellow; conidiophores, glimmer-shaped branches, spherical spores and single cells.
The sequences of the golden basket fungus (Talaromyces aurantiacus) IST, BenA and CaM are shown in SEQ ID No: 1 is shown. And comparing and analyzing the sequences of the ITS, the BenA and the CaM with the sequences in a GeneBank database. The results show that the strain JXBR04 has high homology with Talaromyces (Talaromyces) and has 100 percent of mutual recognition with Talaromyces aurantiacaus. Combined with the ITS, BenA and CaM sequence analysis, the strain is identified as Talaromyces aurantiacas.
The JXBR04 strain can convert insoluble inorganic phosphorus in soil into soluble phosphorus for the moso bamboo to absorb and utilize, thereby promoting the growth of the moso bamboo.
The invention also provides a preparation method of the liquid microbial inoculum, which comprises the following specific operations:
1) inoculating the strain JXBR04 of the golden basket fungus (Talaromyces aurantiacus) to a PDA solid culture medium,
2) after the plate is full of hyphae, perforating the plate with mycelium of JXBR04 of Talaromyces aurantiacas (Talaromyces aurantiacas) with an aseptic perforator,
3) and then, transferring the mycelium cake into a 250ml triangular flask filled with 150ml PDA liquid culture medium by using a needle for propagation, inoculating 10 blocks into each flask, and culturing for 6-8 days at 25 ℃ and 121r/min until the mycelium pellet grows full of the liquid culture medium, namely the liquid microbial inoculum.
Further, the diameter d of the sterile punch is 7 mm.
Further, the air conditioner is provided with a fan,in step 3), before propagation, the PDA liquid culture medium is treated by 1.01 multiplied by 106Sterilizing at Pa and 121 deg.C for 20min, and keeping aseptic state.
Has the advantages that: compared with the prior art, the Talaromyces aurantiacas has the high-efficiency phosphate solubilizing function and also has better potassium solubilizing and IAA secreting capabilities. For insoluble tricalcium phosphate (Ca) under indoor liquid culture conditions3(PO4)2) Iron phosphate (FePO)4) Calcium hydrogen phosphate (CaHPO)4) Aluminum phosphate (AlPO)4) And calcium phytate (organic phytate) have strong dissolving effect; the rhizosphere efficient phosphate-solubilizing golden basket fungus of the moso bamboo is prepared into a liquid microbial inoculum to inoculate annual moso bamboo seedlings. The result shows that the microbial inoculum can obviously promote the growth and development of the moso bamboo, so that the invention provides excellent strain resources for developing the microbial fertilizer special for the moso bamboo in the future.
Drawings
FIG. 1 shows the colony morphology of Sciadophyllum chrysosporium JXBR04 on CYA medium and the phosphorus-solubilizing circles generated on NBRIP medium, Panel A: culturing the basket fungus on CYA culture medium for 7 days to obtain colony morphology; and B, drawing: p-lysing rings were generated by culturing the Scutellaria aurantiaca in NBRIP medium for 7 days.
FIG. 2 shows conidiophores and conidia of the basket fungus, and broom-like molecular conidiophores and elliptical molecular spore forms can be observed from the left, middle and right panels.
FIG. 3 is a phylogenetic tree of the CaM gene sequence of strain JXBR 04.
FIG. 4 shows that strain JXBR04 was used to treat 5 types of calcium phosphate tricalcium phosphate (Ca) with poor solubility under different pH conditions3(PO4)2) Iron phosphate (FePO)4) Calcium hydrogen phosphate (CaHPO)4) Aluminum phosphate (AlPO)4) And the dissolution capacity of calcium phytate.
FIG. 5 shows the potassium-solubilizing ability of strain JXBR 04.
FIG. 6 shows the IAA secretion ability of JXBR 04.
Detailed Description
The present invention will be better understood by those skilled in the art from the following examples. The examples are described only to illustrate the invention and should not be construed as limiting the invention as detailed in the claims.
Example 1: growth test of JXBR04 Strain under acid stress
Activated strain JXBR04 is inoculated into PDA liquid culture medium (the formula of the PDA liquid culture medium is that potato 200g, glucose 20g and distilled water 1000mL), shaking culture is carried out at 25 ℃ and 120r/min for 5d to serve as seed liquid, the pH of the PDA liquid culture medium is set to be 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, the seed liquid is divided into 250mL triangular flasks, and the liquid filling amount is 100 mL. Subsequently, the seed solutions were inoculated into PDA liquid medium in an inoculum size of 1% (v/v), and the treatment was repeated 3 times, and shaking culture was carried out at 25 ℃ and 120r/min for 8 d. Filtering the mycelium, drying at 65 ℃ for 48h, and weighing. The results are shown in Table 1, the strain JXBR04 grows better under different acidic conditions of pH 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, the hypha biomass is not different, and the pH range of the fermentation liquor is 3.40-3.95. The results show that the strain JXBR04 can regulate the self-appropriate growth acid environment by secreting acid substances, is further suitable for different acid environments, and has the ability of growing in different acid soil environments.
TABLE 1 Effect of different pH on JXBR04 hyphal biomass and broth pH
Note: p is less than 0.05, and the small and the letter of different rows in the same column represent the difference
Example 2: strain JXBR04 was tested for phosphate solubilization under acid stress.
Phosphate solubilizing medium a: glucose 10g, Ca3(PO4)2 5g，MgCl2 5g，KCl 0.2g，MgSO4.7H2O 0.25g，(NH4)28O40.1g, and 1000mL of distilled water.
Phosphate solubilizing medium B: with aluminium phosphate (AlPO)4) Replace Ca in the phosphate solubilizing medium A3(PO4)3Other ingredientsAnd the content is the same.
Phosphate solubilizing medium C: from iron phosphate (FePO)4) Replace Ca in the phosphate solubilizing medium A3(PO4)3And other components and contents are the same.
Phosphate solubilizing medium D: with calcium hydrogen phosphate (CaHPO)4) Replace Ca in the phosphate solubilizing medium A3(PO4)3And other components and contents are the same.
Phosphate solubilizing medium E: with calcium phytate (C)6H6Ca6O24P6) Replace Ca in the phosphate solubilizing medium A3(PO4)3And other components and contents are the same.
Inoculating activated JXBR04 strain into PDA culture medium (the formula of PDA culture medium is 200g of potato, 20g of glucose, 18g of agar and 1000mL of distilled water), culturing with shaking at 25 ℃ and 120r/min for 5d to serve as seed liquid, respectively inoculating the seed liquid into 100mL triangular flasks containing 50mL of phosphate solubilizing culture medium A, B, C, D and E with the inoculation amount of 1% (v/v) and the pH value respectively adjusted to 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, respectively, treating the phosphate solubilizing culture medium with the same volume of blank seed liquid as Control (CK) for three times, culturing at 25 ℃ and 121r/min for 8d, centrifuging the fermentation liquid at 4 ℃, 10000r/min for 10min, determining the content of soluble phosphorus in the fermentation liquid by molybdenum-antimony colorimetric method,
the results are shown in FIG. 4. As can be seen from FIG. 4, tricalcium phosphate (Ca), a sparingly soluble phosphate, was used3(PO4)2) Iron phosphate (FePO)4) Calcium hydrogen phosphate (CaHPO)4) Aluminum phosphate (AlPO)4) And calcium phytate (C)6H6Ca6O24P6) As the only phosphorus source, after the strain is cultured for 8 days in different acidic environments with pH values of 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, the content of soluble phosphorus in the JXBR04 fermentation liquor is obviously higher than that of CK in different acidic environments. The results show that the strain JXBR04 has strong dissolving capacity on five indissolvable phosphates under different acidic conditions.
Example 3: JXBR04 strain potassium-dissolving capacity determination test.
The JXBR04 strain is inoculated into a potassium-dissolving culture medium (potassium-dissolving culture) containing 50mLThe formula of the base is as follows: potassium feldspar powder 0.1g, Na2HPO4 2.0g，FeCl3 0.005g，MgSO4·7H20.5g of O, 5.0g of cane sugar and Ca2CO30.1g, agar 18.0g, distilled water 1000mL, pH 7.0. ) Performing shake culture for 7d at 28 deg.C and 180r/min in 100mL triangular flask to obtain fermentation liquid, removing insoluble substances from the fermentation liquid at 500r/min for 10min, centrifuging at 10000r/min for 10min to collect supernatant, and measuring the content of effective potassium in the supernatant by flame spectrophotometer. As shown in FIG. 5, JXBR04 showed a potassium-releasing amount of 15.28mg/L, which was superior in potassium-releasing ability.
Example 4: strain JXBR04 was tested for IAA-producing ability.
The IAA production capacity was determined by Salkowski colorimetry. Taking IAA standard, configuring into concentration gradient of 0, 0.5, 2.5, 5.0, 7.5, 10, 12.5, 15.0, 17.5, 25.0 and 50.0mg/L, taking 2mL of IAA with each concentration, adding equal amount of ferric chloride colorimetric solution (PC colorimetric solution), keeping the temperature in the dark at 30 ℃ for 30min, measuring absorbance at the wavelength of 530nm by using a spectrophotometer, drawing a standard curve, and obtaining the equation y which is 31.868x (R)20.9947). The activated strain JXBR04 was inoculated into King's medium (formula: peptone 2g glycerol 1g, K)25O4 0.15g、MgSO4 4.7g、H20.15g of O, 1.5g of agar and H2O1000 ml, pH 7.2) and shake culturing for 15d, and measuring the IAA content in the fermentation liquor according to the standard curve making method. As shown in FIG. 6, JXBR04 has better IAA production capacity, and the IAA secretion amount is 21.25 mg/L.
Example 5: JXBR04 greenhouse potting test:
(1) cultivation of moso bamboo bud seedlings
Soaking moso bamboo seeds in 0.5% potassium permanganate solution for 2h for surface disinfection, washing with tap water for 1h, sowing in sterilized sand, culturing in a greenhouse at 25 deg.C, and cutting bud seedling and transplanting when the moso bamboo seeds grow to cotyledon stage.
(2) The potting matrix soil is collected from the hind hill of the university of Jiangxi agriculture, sterilized at 165 ℃ for 2h according to the mass ratio of soil, sand and vermiculite of 2: 1, and cooled for later use.
(3) Preparation of liquid microbial inoculum
Inoculating the stored golden basket fungus strain into PDA solid culture medium (the formula of PDA solid culture medium is glucose 20g, potato juice 200g, agar 18g, water 1000mL), culturing at 25 deg.C for 6d, making the hypha overgrow the plate, beating the colony edge into fungus cake with a punch (d is 7mm) under aseptic condition, and transferring into 500mL triangular flask containing 250mL of PDA liquid culture medium (the formula of PDA liquid culture medium is potato 200g, glucose 20g, distilled water 1000mL) for propagation (the PDA liquid culture medium is subjected to 1.01 × 106Sterilizing at Pa and 121 deg.C for 20min, and keeping aseptic state); 10 fungus cakes were picked per bottle. Placing at a culture temperature of 25 ℃ and a culture rotation speed of 120 r.min-1Culturing under the culture condition until the mycelium is in a vigorous stage, and pulverizing with a sterilized homogenizer to obtain the liquid microbial inoculum.
(4) Potted plant test by liquid microbial inoculum inoculation
Transplanting the moso bamboo seedlings by adopting a bud seedling root cutting method, wherein the inoculation amount of the microbial inoculum is 10ml per plant. The specific method comprises the following steps: taking a proper amount of sterile potting medium, filling the potting medium into a flowerpot, gently taking out the moso bamboo seedlings in the cotyledon period from a seedling pot, preventing the roots of the moso bamboo seedlings from being damaged as far as possible, washing sand at the roots of the seedlings with clear water, cutting off a small amount of main roots of the seedlings by using a scalpel, transplanting the seedlings into the flowerpot, putting a microbial inoculum around the moso bamboo rhizosphere, compacting soil, covering with matrix soil, watering and fixing the roots. The control was made of two treatments, inoculation of liquid medium only and no inoculation of bacteria, 10 replicates for each treatment. Culturing in 25 deg.C greenhouse, watering at proper time, and managing uniformly. The growth condition of 180d after moso bamboo inoculation is shown in table 2, and it can be seen that the growth of moso bamboo seedlings can be remarkably promoted by the inoculation of JXBR 04. Compared with a Control (CK), the growth rate of the inoculated JXBR04 on the height of the seedlings and the stem thickness respectively reaches 30.95 percent and 28.57 percent, and the growth promoting effect is obvious.
TABLE 2 Effect of Strain JXBR04 on the growth of Phyllostachys Pubescens seedlings
Note: p is less than 0.05, and the lower case letters of different rows in the same column are different to represent difference display
Sequence listing
<110> university of agriculture in Jiangxi
<120> Talaromyces aurantiaca and application thereof
<130> 2017
<160> 3
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gcagttataa tccgacacca tctgatcatc aacagctaca atggctcctc cgacctccag 180
ttggagcgta tgaacgtcta cttcaacgag gtgtgtggaa tcaaccatca gaaaacccat 240
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Claims (10)
1. A fungus, JINHUANGBASIU, is classified and named as JINHUANGBASIU (A)Talaromyces aurantiacus) The strain number is JXBR04, the strain is preserved in China center for type culture Collection, and the preservation number is: CCTCC NO: m2017327, the preservation date is 6 months and 13 days in 2017.
2. Use of the golden basket fungus of claim 1 for promoting the growth of moso bamboos.
3. The use of claim 2, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) Use of JXBR04 for the solubilization of poorly soluble phosphates.
4. The use of claim 3, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) JXBR04 dissolves insoluble phosphate in a gradient manner at a pH of 1.5-6.5.
5. Use according to claim 3, wherein the poorly soluble phosphates comprise tricalcium phosphate, iron phosphate, aluminium phosphate, calcium hydrogen phosphate, calcium phytate.
6. The use of claim 2, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) Use of JXBR04 for dissolving poorly soluble potassium.
7. The use of claim 2, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) JXBR04 has the function of secreting IAA.
8. The method for preparing the liquid microbial inoculum by utilizing the golden basket fungus of claim 1, which comprises the following steps:
1) the above-mentioned Talaromyces aureobasicola (A), (B), (CTalaromyces aurantiacus) JXBR04 strain is inoculated on PDA solid culture medium,
2) after the hyphae grow over the plate, the basket fungus with golden yellow is put in an aseptic puncherTalaromyces aurantiacus) Plates of JXBR04 hyphae were perforated,
3) and then, transferring the mycelium cake into a 250ml triangular flask filled with 150ml PDA liquid culture medium by using a needle for propagation, inoculating 10 blocks into each flask, and culturing for 6-8 days at 25 ℃ and 121r/min until the mycelium pellet grows full of the liquid culture medium, namely the liquid microbial inoculum.
9. The method for preparing liquid bacterial agent according to claim 8, wherein in step 3), PDA liquid culture medium is passed through 1.01 x 10 before propagation6 Sterilizing at Pa and 121 ℃ for 20 min.
10. The method for preparing liquid microbial inoculum according to claim 8, wherein the PDA solid culture medium has the following formula: 20g of glucose, 200g of potato juice, 18g of agar and 1000mL of water; the PDA liquid culture medium comprises the following components: 200g of potato juice, 20g of glucose and 1000mL of distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710946541.7A CN107858293B (en) | 2017-10-11 | 2017-10-11 | Talaromyces aurantiaca and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN112812977B (en) * | 2021-03-08 | 2023-03-24 | 哈尔滨师范大学 | Phosphorus-dissolving fungus and application thereof |
| CN113717860B (en) * | 2021-07-07 | 2023-05-16 | 昆明理工大学 | Application of Huang Lanzhuang bacteria in conversion of total saponins of Notoginseng radix into small-polarity ginsenoside |
| CN115369043B (en) * | 2021-12-14 | 2023-06-16 | 贵州民族大学 | Multifunctional basket strain GYDW-YM101 and application thereof |
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