CN103060242B - Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof - Google Patents
Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof Download PDFInfo
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Abstract
The invention discloses a bacillus fusiformis for promoting rhizosphere growth of blueberries. The bacterial strain is named as bacillus fusiformis (Bacillus fusiformis) L13 in class, and preserved at the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 31, 2012; and the preservation number is CGMCC No.7068. The invention also provides application of the bacillus fusiformis for promoting rhizosphere growth of the blueberries. The bacillus fusiformis for promoting rhizosphere growth of the blueberries disclosed by the invention is screened from rhizosphere soil of the blueberries, adapts with a special rhizosphere soil environment of the blueberries, and can promote growth of the blueberries.
Description
Technical field
The present invention relates to strain blueberry rhizosphere growth-promoting bacillus fusiformis and an application thereof.
Background technology
Blueberry (Vaccinum ssp.), be called again blueberry, cowberry, blue berry, is Ericaceae (Ericaceae) genus vaccinium (Vaccinium L.) plant.Blueberry is berry, be rich in the anti-oxidant compounds such as various VITAMIN, arbutin, cyanin, SOD, tanning acid and flavonoid, be that a kind of utility value at first grown up in the U.S. is high, remarkable in economical benefits, have the nutritive health-care fruit of high nutritive value, health-care effect and pharmaceutical use.International food and agricultural organization classifies them one of as the mankind's five large heath food, is the treasure in fruit tree.
Blueberry is the oligotrophic plant, and too much fertilising or improper fertilization easily cause setting body and come to harm.And the fertilizer used during at present blueberry plantation is traditional fruit tree fertilizer mostly, not only specific aim is poor, also is easy to cause soil compaction, very large on the impact of blueberry quality.Microbial fertilizer can not only be improved soil, improves the ecotope of soil fauna, can also effectively improve the absorption of plant to soil nutrient.Chinese patent CN201110128890.0 discloses blueberry mycorrhizal fungi (Coprinus micaceus) and separation method and purposes, related generally to a strain from separate the wild Vaccinium root system of plant in Changbai Mountain obtain be used to the mycorrhizal fungi that inoculates blueberry and promoting the blueberry growth and improving the application aspect output.Chinese patent CN201010180670.8 discloses blueberry and has cultivated and plant by probiotics, preparation method and using method, is after the purifying cultivation, each bacterium liquid mixed preparing to be formed respectively with organophosphorus bacterium, bacillusmusilaginosiengineering, lactobacillus, bacillus cereus, photosynthetic bacteria, nitrogen-fixing rhizobia, candiyeast and filamentous fungus.Yet there are no the rhizosphere growth-promoting endophytic bacteria screening of blueberry special use and the report of application.
Plant rhizosphere growth-promoting bacterium refers to that existence is in roots of plants circle scope, plant-growth is had to the beneficial bacteria general designation that promotes or pathogenic bacteria is had to antagonistic action, and plant-growth and disease control are had to extremely important effect.Plant rhizosphere growth-promoting bacterium can secrete the plant growth-promoting material, comprises plant hormone, VITAMIN, amino acid and other active organic molecule and derivative etc.; Also can improve the nutrient environment of plant rhizosphere, effectively control Plant diseases etc.According to incompletely statistics, found to comprise that more than 20 plant rhizosphere growth-promoting bacteriums that belong to such as pseudomonas and bud pole bacterium have the report of disease prevention growth-promoting potential.But plant rhizosphere growth-promoting bacterium has very strong specificity, and the blueberry growth conditions is more special again, only suitable to the high acid soil of organic content (4.0～4.8) growth, and most microorganisms can not normal growth under pH4.0～4.8 environment.Therefore, screening is exclusively used in the rhizosphere growth-promoting endophytic bacteria of blueberry, and output and the quality that promotes blueberry growth, raising blueberry had to important meaning.
Summary of the invention
The objective of the invention is for overcoming above-mentioned the deficiencies in the prior art, strain blueberry rhizosphere growth-promoting bacillus fusiformis and an application thereof is provided.
The present invention is achieved by the following technical solutions:
One strain blueberry rhizosphere growth-promoting bacillus fusiformis, this strain classification called after bacillus fusiformis (Bacillus fusiformis) L13, on December 31st, 2012, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.7068.
Colony characteristics and the thalli morphology of described blueberry rhizosphere growth-promoting bacillus fusiformis:
Bacillus fusiformis L13 forms the oyster white bacterium colony after on the LB flat board, cultivating 24h, circle, and opaque, neat in edge is smooth, and there is the projection fold centre, and somatic cells size is the μ m of (0.5-2.5) μ m * (1.2-10), product gemma, G
+, it is shaft-like that cell is.
The physiological and biochemical property of described blueberry rhizosphere growth-promoting bacillus fusiformis:
The Starch Hydrolysis negative, the gelatin liquification test positive, it is negative that glucose produces acid, glucose aerogenesis feminine gender, Fu-Pu negative, the methyl red test feminine gender, the indole test feminine gender, the nitrate reduction experiment is negative.
By the sequence alignment in described blueberry rhizosphere growth-promoting bacillus fusiformis 16S rDNA sequence and GenBank database, result shows: L13 and Bacillus fusiformis are in together in a minimum branch, and the similarity of its 16S rDNA sequence and Bacillusfusiformis (AF169537) reaches 98%.In conjunction with colonial morphology, physiological and biochemical property and 16S rDNA sequential analysis, be accredited as bacillus fusiformis (Bacillus fusiformis).
Described blueberry rhizosphere growth-promoting bacillus fusiformis can produce indolylacetic acid (IAA), and coordinate plant growth and growth are had to vital role.
Beneficial effect of the present invention:
After microbial inoculum is made in blueberry rhizosphere growth-promoting bacillus fusiformis screening of the present invention, be inoculated into the blueberry seedling, can obviously promote the blueberry root growth, the plant height and the stem that increase blueberry are thick, and compared with the control, difference reaches conspicuous level.Therefore, the present invention provides good strain resource for the microbial fertilizer of developing in the future the blueberry special use.
Embodiment
The present invention is further described below in conjunction with embodiment.
One strain blueberry rhizosphere growth-promoting bacillus fusiformis, this strain classification called after bacillus fusiformis (Bacillus fusiformis) L13, on December 31st, 2012, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.7068.
The screening of embodiment 1 blueberry rhizosphere growth-promoting bacillus fusiformis
(1) screening process:
By the gradient dilution method, from the blueberry Rhizosphere sampling of fertile woods blueberry production base, Jiaonan City, Shandong Province, isolating 216 strain isolates.By three zoning collimation methods, the bacterium be separated to is carried out to purifying, and judge whether purifying of bacterial strain by microscopy, the bacterium after purifying is numbered, picking list bacterium colony, be transferred on the LB inclined-plane and save backup.By protecting green method, filter out the green effect of 14 strains guarantor bacterial strain preferably, in conjunction with radish cotyledon weightening finish method, finishing screen is selected the bacterium that 1 strain has good growth-promoting effect, is designated as L13.
(2) colony characteristics and thalli morphology:
Bacillus fusiformis L13 forms the oyster white bacterium colony after on the LB flat board, cultivating 24h, circle, and opaque, neat in edge is smooth, and there is the projection fold centre, and somatic cells size is the μ m of (0.5-2.5) μ m * (1.2-10), product gemma, G
+, it is shaft-like that cell is.
(3) physiological and biochemical property:
The Starch Hydrolysis negative, the gelatin liquification test positive, it is negative that glucose produces acid, glucose aerogenesis feminine gender, Fu-Pu negative, the methyl red test feminine gender, the indole test feminine gender, the nitrate reduction experiment is negative.
(4) producing indolylacetic acid (IAA) ability measures
Bacillus fusiformis L13 is inoculated in to the LB substratum, and 28 ℃, the 180rpm shaking table is cultivated 4d.By the OD600 value of spectrophotometry bacteria suspension, then by the centrifugal 10min of bacteria suspension 10000rpm, get supernatant liquor and add equal-volume Salkowski (50mL35%HClO
4+ 1mL0.5M FeCl
3) color solution, the standing 30min of lucifuge, measure its OD530 value.Calculating bacteria concentration OD600 value is 1 o'clock, the content of IAA in the unit volume fermented liquid, the content of the typical curve calculating IAA by the IAA gradient dilution.By measuring, it is 17.38 μ g/ (mLOD600) that bacillus fusiformis L13 produces IAA content
-1.
(5) 16S rDNA sequential analysis
16S rDNA gene order, be shown in shown in the nucleotides sequence list.By the sequence alignment in survey 16S rDNA sequence and GenBank database, result shows: L13 and Bacillus fusiformis are in together in a minimum branch, and the similarity of its 16S rDNA sequence and Bacillus fusiformis (AF169537) reaches 98%.In conjunction with colonial morphology, physiological and biochemical property and 16SrDNA sequential analysis, be accredited as bacillus fusiformis (Bacillus fusiformis).
Growth has growth-promoting functions to embodiment 2 blueberry rhizosphere growth-promoting bacillus fusiformis to blueberry
The blueberry rhizosphere growth-promoting bacillus fusiformis L13 of preservation is activated on the LB inclined-plane, picking one articulating enters beef-protein medium (extractum carnis 0.3%, peptone 1%, sodium-chlor 0.5%, agar 2%, pH7.0-7.2, more than be mass percent) in, in 37 ℃, under the 180rpm condition, shaking culture is 2 days.After centrifugal 5min under fermented liquid 6000rpm, stroke-physiological saline solution rinse thalline 3 times, stroke-physiological saline solution is regulated bacteria suspension (2.0 * 10
8Cfu/m) make Inoculant.By after the field planting of blueberry container seedling, get 20ml and evenly water around the blueberry root with 10 times of sterilized water dilutions.With pouring equivalent stroke-physiological saline solution in contrast, after three months, measure root system, height of seedling and the leading thread situation of blueberry, it is as shown in the table for result.
As can be seen from the table, the blueberry adventive root length that blueberry rhizosphere growth-promoting bacillus fusiformis suspension is processed, adventive root stem are thick, plant height and stem rough segmentation have not improved 14.40%, 12.04%, 11.23% and 9.95% than contrast, and difference all reaches conspicuous level.More than show: bacterial strain L13 has obvious promoter action to the growth of blueberry.
More than adventive root refers to length 10cm, more than base diameter 0.1cm, the root system that length 10cm is above; Adventive root stem slightly refers to the rugosity at the 2cm of foundation section place; Plant height refers to from the length on Dao Jing top, earth's surface, the rugosity at 5cm place, the thick span of stem ground.In same row, same letter means that difference is not remarkable, otherwise means significant difference (p<0.05).
Claims (2)
1. a strain blueberry rhizosphere growth-promoting bacillus fusiformis, it is characterized in that: this strain classification called after bacillus fusiformis (Bacillus fusiformis) L13, on December 31st, 2012, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.7068.
2. blueberry rhizosphere growth-promoting bacillus fusiformis claimed in claim 1 application in promoting the blueberry growth.
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| CN103215210B (en) * | 2013-05-08 | 2014-08-27 | 青岛海大生物集团有限公司 | Bacillus siamensis and application of bacillus siamensis in preparation of microbial seaweed fertilizer |
| CN103396262B (en) * | 2013-07-24 | 2015-09-30 | 青岛蓝莓生物科技有限公司 | Blueberry plantation special-purpose soil modifying agent |
| CN104788177B (en) * | 2015-03-31 | 2016-03-02 | 山东省林业科学研究院 | Special water conservation biological organic fertilizer of blueberry and preparation method thereof |
| CN104745514B (en) * | 2015-04-01 | 2016-03-02 | 山东省林业科学研究院 | Blueberry rhizosphere growth-promoting Bacillus cereus and composite fungus agent thereof and application |
| CN105779364B (en) * | 2016-05-17 | 2017-10-13 | 山东省林业科学研究院 | One plant of bacillus fusiformis and its application in drought mountain afforestation |
| CN106857171A (en) * | 2017-04-14 | 2017-06-20 | 黄平县华富生态种养殖科技发展有限公司 | The implantation methods of many mycorhiza blueberries |
| CN113278563A (en) * | 2021-06-28 | 2021-08-20 | 丰德生物技术(大连)有限公司 | Special soil improvement microbial agent for strawberries |
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Non-Patent Citations (6)
| Title |
|---|
| 《Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea》;Park M等;《Microbiology Research》;20050425;第160卷(第2期);摘要，结果和讨论 * |
| Jiang,D.,等.《Bacillus fusiformis strain LQ104 16s ribosomal RNA gene, partial sequence》.《GenBank登录号:EF472267》.2007,全文. * |
| Jiang,D.,等.《Bacillus fusiformis strain LQ88 16s ribosomal RNA gene, partial sequence》.《GenBank登录号：EF472269》.2007,全文. * |
| Nakamura,L.K..《Bacillus fusiformis 16s ribosomal RNA genepartial sequence》.《GenBank登录号:AF169537》.2000,全文. * |
| Park M等.《Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea》.《Microbiology Research》.2005,第160卷(第2期),摘要，结果和讨论. |
| Wang,X.,等.《Lysinibacillus fusiformis strain R6 16S ribosomal RNA gene,partial sequence 》.《GenBank登录号：HQ259955》.2011,全文. * |
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