CN110257263A - The basket bacterium SBS-ZGTP02 of one plant of pinus sylvestris var. mongolica rhizosphere tool wart and its application - Google Patents
The basket bacterium SBS-ZGTP02 of one plant of pinus sylvestris var. mongolica rhizosphere tool wart and its application Download PDFInfo
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- CN110257263A CN110257263A CN201910671285.4A CN201910671285A CN110257263A CN 110257263 A CN110257263 A CN 110257263A CN 201910671285 A CN201910671285 A CN 201910671285A CN 110257263 A CN110257263 A CN 110257263A
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- mongolica
- pinus sylvestris
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Abstract
The present invention provides the basket bacterium SBS-ZGTP02 of one plant of pinus sylvestris var. mongolica rhizosphere tool wart and its applications, belong to microorganisms technical field, and Latin is Talaromyces verruculosus, and deposit number is CGMCC No.17768.The dissolution of insoluble phosphate can be improved in the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart provided by the invention, promotes the growth of pinus sylvestris seedling, improves pinus sylvestris var. mongolica rhizosphere soil and physicochemical character, and improve the enzymatic activity in pinus sylvestris var. mongolica rhizosphere soil.
Description
Technical field
The invention belongs to microorganisms technical field more particularly to the basket bacterium SBS-ZGTP02 of one plant of pinus sylvestris var. mongolica rhizosphere tool wart and
It is applied.
Background technique
Phosphorus is one of big nutrient of plant growth necessary three, and the available available phosphorus Zhan of plant is total under natural conditions
The very small part of phosphorus content.Phosphate solubilizing microorganism refer to indissoluble phosphorus can transform into plant can be absorbed and utilized in soil can
Dissolubility phosphorus promotes plant growth, improves its resistance to improve plant to the absorption efficiency of rhizosphere phosphorus.Soluble phosphorus microorganism packet
Bacterium, fungi and actinomyces are included, the phenomenon that there are phosphorus decomposing efficiency degradations in squamous subculture due to bacterium and actinomyces, and phosphorus decomposing
Fungi is relatively stable, stronger to the rhizosphere adaptability under natural conditions after preparing microbial inoculum use, therefore has higher exploitation benefit
With value.Pinus sylvestris var. mongolica is northern China Major Tree Species Planted, has resistance strong, rapid feature is grown, in restoration of the ecosystem, garden
Woods greening, check winds and fix drifting sand etc. play an important role, and there are Pesticide uses excessively to cause in pinus sylvestris seedling production at present
Pathogen develops drug resistance, soil micro-ecosystem structure is destroyed, the bad problem of environmental pollution, nursery stock growing way, and probiotics makes
With can promote seedling growth, improve nursery stock disease resistance, improve rhizosphere environment in terms of play a significant role, phosphorus decomposing fungi
Belong to plants probiotics, and in the prior art not about the report for the phosphorus decomposing fungi for being adapted to pinus sylvestris var. mongolica rhizosphere.
Summary of the invention
In view of this, the purpose of the present invention is to provide the basket bacterium SBS-ZGTP02 of one plant of pinus sylvestris var. mongolica rhizosphere tool wart and its answering
With the dissolution of insoluble phosphate can be improved in the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart provided by the invention, promotes pinus sylvestris seedling
Growth improves pinus sylvestris var. mongolica rhizosphere soil and physicochemical character, and improves the enzymatic activity in pinus sylvestris var. mongolica rhizosphere soil.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical schemes:
The present invention provides one plant of pinus sylvestris var. mongolica rhizospheres to have the basket bacterium SBS-ZGTP02 of wart, Latin Talaromyces
Verruculosus, deposit number are CGMCC No.17768.
The present invention also provides the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in above-mentioned technical proposal to improve slightly solubility phosphoric acid
Application in salt dissolution.
Preferably, the insoluble phosphate includes one or more of calcium phosphate, aluminum phosphate and ferric phosphate.
The present invention also provides the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in above-mentioned technical proposal to promote pinus sylvestris seedling
Application in growth.
The present invention also provides the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in above-mentioned technical proposal to improve pinus sylvestris var. mongolica rhizosphere
Application in the physicochemical character of soil.
Preferably, the physicochemical character includes the soil organism, full nitrogen, available nitrogen, full phosphorus, rapid available phosphorus, full potassium and quick-acting
Potassium.
The present invention also provides described in above-mentioned technical proposal pinus sylvestris var. mongolica rhizosphere tool the basket bacterium of wart improve pinus sylvestris var. mongolica according to
Application in Enzyme Activities in Soils.
Preferably, the enzyme includes one or more of invertase, catalase, acid phosphatase and urase.
The present invention provides the basket bacterium SBS-ZGTP02 of one plant of pinus sylvestris var. mongolica rhizosphere tool wart and its application, Latin to be
Talaromyces verruculosus, deposit number are CGMCC No.17768.Pinus sylvestris var. mongolica rhizosphere provided by the invention has wart
Basket bacterium improves the dissolution of insoluble phosphate, promotes the growth of pinus sylvestris seedling, improves pinus sylvestris var. mongolica rhizosphere soil and physics and chemistry
Shape, and improve the enzymatic activity in pinus sylvestris var. mongolica rhizosphere soil.
Biological deposits prove
Has the basket bacterium SBS-ZGTP02 of wart, Latin was Talaromyces verruculosus, in 06 month 2019 03
Day is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is BeiChen West Road, Chaoyang District, BeiJing City
No. 1 institute 3, deposit number CGMCCNo.17768.
Detailed description of the invention
Fig. 1 is the tool basket bacterium SBS-ZGTP02 of wart phosphate solubilization on NBRIP culture medium, wherein being followed successively by from left to right 1)
The phosphate solubilization on the NBRIP culture medium of covering cellophane membrane；2) remove the transparent circle after cellophane membrane on NBRIP culture medium；
3) transparent circle that the tool basket bacterium SBS-ZGTP02 of wart is directly formed on NBRIP culture medium；
Fig. 2 is to have the basket bacterium SBS-ZGTP02 of wart to the growth-promoting functions of pinus sylvestris seedling, wherein at nursery stock from left to right
Reason is successively are as follows: 1) SBS-ZGTP02 solid fungicide；2) cotton seed hulls compares 3) blank control.
Specific embodiment
The present invention provides one plant of pinus sylvestris var. mongolica rhizospheres to have the basket bacterium SBS-ZGTP02 of wart, Latin Talaromyces
Verruculosus, deposit number are CGMCC No.17768.In the present invention, the basket bacterium of the pinus sylvestris var. mongolica rhizosphere tool wart is improved
The dissolution of insoluble phosphate promotes the growth of pinus sylvestris seedling, improves pinus sylvestris var. mongolica rhizosphere soil and physicochemical character, and improve camphor tree
Enzymatic activity in sub- pine rhizosphere soil.
The present invention also provides the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in above-mentioned technical proposal to improve slightly solubility phosphoric acid
Application in salt dissolution.In the present invention, the insoluble phosphate include one of calcium phosphate, aluminum phosphate and ferric phosphate or
It is several.
The present invention also provides the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in above-mentioned technical proposal to promote pinus sylvestris seedling
Application in growth.In the present invention, the application preferably includes: solid bacterium is made in the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart
After agent, it is applied in pinus sylvestris var. mongolica rhizosphere soil.The present invention is not particularly limited the preparation method of the solid fungicide, using normal
Regulation Preparation Method has the work spore content of the basket bacterium of wart in the solid fungicide 4.0 × 108A/g or more.
The present invention also provides the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in above-mentioned technical proposal to improve pinus sylvestris var. mongolica rhizosphere
Application in the physicochemical character of soil.In the present invention, the physicochemical character preferably include the soil organism, full nitrogen, available nitrogen,
Full phosphorus, rapid available phosphorus, full potassium and available potassium, the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart can be improved the soil organism, full nitrogen, quick-acting
The content of nitrogen, full phosphorus, rapid available phosphorus, full potassium and available potassium in pinus sylvestris var. mongolica rhizosphere soil.In the present invention, the preferred packet of the application
It includes: after solid fungicide is made in the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart, being applied in pinus sylvestris var. mongolica rhizosphere soil.The present invention is to institute
The preparation method for stating solid fungicide is not particularly limited, using customary preparation methods, pinus sylvestris var. mongolica root in the solid fungicide
Border has the work spore content of the basket bacterium of wart 4.0 × 108A/g or more.
The present invention also provides described in above-mentioned technical proposal pinus sylvestris var. mongolica rhizosphere tool the basket bacterium of wart improve pinus sylvestris var. mongolica according to
Application in Enzyme Activities in Soils.In the present invention, the enzyme preferably include invertase, catalase, acid phosphatase and
One or more of urase.In the present invention, the application preferably includes: the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart is made
After solid fungicide, it is applied in pinus sylvestris var. mongolica rhizosphere soil.The present invention is not particularly limited the preparation method of the solid fungicide,
Using customary preparation methods, the work spore content of the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart is 4.0 × 10 in the solid fungicide8
A/g or more.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
In following instance,
The formula of PDA culture medium are as follows: potato 200g, glucose 20g, agar powder 18g, distilled water 1000mL.
The formula of phosphorus decomposing culture medium (NBRIP) are as follows: glucose 10g, Ca3(PO4)25g, MgCl2.6H2O 5g, KCl
0.2g, MgSO4.7H2O 0.25g, (NH4)2SO40.1g, distilled water 1000mL, pH7.0.
The formula of phosphorus decomposing culture medium A are as follows: use aluminum phosphate (AlPO4) instead of the Ca in NBRIP3(PO4)2, other compositions and contain
It measures identical.
The formula of phosphorus decomposing culture medium B are as follows: use ferric phosphate (FePO4·4H2O the Ca in NBRIP) is replaced3(PO4)2, other at
Divide identical with content.
Cotton seed hulls solid medium: cotton seed hulls 1000g, glucose 20g, magnesium sulfate 2.5g, potassium dihydrogen phosphate 7.5g, lime
Powder 5g adds water to humidity 60%.
Embodiment 1
Has the phosphorus decomposing of the basket bacterium of wart (Talaromyces verruculosus) SBS-ZGTP02
1) the phosphorus decomposing circle of solid plate Conditions Strains
To have the basket bacterium of wart (Talaromyces verruculosus) SBS-ZGTP02 to be inoculated on NBRIP plate, 25
DEG C culture, after 7d, has seen whether transparent circle, has measured the diameter of bacterium colony (D) and transparent circle (d), and calculate transparent loop diameter and
The ratio (d/D) of colony diameter, the dissolving P capacity of estimation phosphorus decomposing fungi that can be preliminary.Ratio is bigger, illustrates the phosphorus decomposing of fungi
Ability is stronger.The value of the d/D of the measurement tool basket bacterium of wart according to the above method, as a result measuring ratio is 1.55, illustrates have the basket bacterium of wart
Bacterial strain has very strong dissolving P capacity.The result is shown in Figure 1.
2) phosphorus decomposing of the liquid fermentation condition bacterial strain to slightly solubility inorganic phosphate
To have in the basket bacterium of wart (Talaromyces verruculosus) SBS-ZGTP02 strain inoculated to PDA plate,
After 25 DEG C of culture 7d, fungus block (diameter 10mm) is taken to be inoculated into respectively equipped with 100mLNBRIP liquid in plate with the punch of sterilizing
In the triangular flask of the 250mL of culture medium and phosphorus decomposing fluid nutrient medium A, B, the phosphorus decomposing culture medium for not connecing bacterium with same volume respectively is
It compares (CK), 3 fungus blocks of every bottle of inoculation, each strain to be tested sets three repetitions, and 25 DEG C, 150rpm shaken cultivation after 7d, will be sent out
Zymotic fluid centrifugation 10min (4 DEG C, 10000rmin-1)；Using P-Mo blue spectrophotometry, that is, the supernatant that takes above-mentioned centrifugation to obtain
100 μ L be added 50mL volumetric flask in, after the deionized water of about 25mL is then added, then plus 1-2 drop 2,4- dinitrophenol dinitrophenolate indicate
Agent, then plus the anti-color developing agent of 5mL molybdenum antimony, then plus deionized water constant volume, mix, stand 0.5h after, measure OD value.And calculate supernatant
Available phosphorus content and phosphorus decomposing rate in liquid.
Phosphorus decomposing rate (%)=(connecing bacterium titanium pigment content-control titanium pigment content)/phosphatic amount × 100 of addition.
It the results are shown in Table 1.
1 bacterial strain SBS-ZGTP02 of table is to insoluble phosphate calcium phosphate, ferric phosphate, aluminum phosphate solvability
As can be drawn from Table 1, respectively with insoluble phosphate tricalcium phosphate, aluminum phosphate, ferric phosphate for unique phosphorus source, culture
After 7d, solvable phosphorus content is above control in fermentation liquid, but significantly different to the solute effect of each phosphorus source, wherein to tricalcium phosphate
Solute effect it is preferable, it is slightly weak to the solute effect of ferric phosphate and aluminum phosphate.
3) condition of culture is to bacterial strain SBS-ZGTP02 phosphorus decomposing influential effect
By the phosphorus decomposing effect (table 2) of bacterial strain SBS-ZGTP02 under measurement different carbon source, nitrogen source, temperature and pH value condition,
It is nitrogen source by carbon source, ammonium sulfate of glucose, fermentation medium pH value is 7.0, and under the conditions of 25 DEG C, phosphorus decomposing effect is best, makes
Phosphorus decomposing is carried out with the culture medium prescription after optimization, phosphorus decomposing value reaches 697.15mg/L.
Influence of the different condition of culture of table 2 to bacterial strain SBS-ZGTP02 phosphorus decomposing effect
Embodiment 2
Has the basket bacterium of wart (Talaromyces verruculosus) SBS-ZGTP02 to the growth-promoting of pinus sylvestris seedling
Solid fungicide preparation: after bacterial strain SBS-ZGTP02 is activated 5d in PDA culture medium, with aseptic card punch (diameter
10mm) go 2 pure culture biscuits involvng inoculations in the triangular flask (500mL) for filling 250mLPD fluid nutrient medium, be placed on shaking table (25 DEG C,
150r/min) after shaken cultivation 7d, cultured liquid spawn is inoculated on cotton seed hulls solid medium, 25 DEG C of dark conditions
Lower stationary culture 15d takes out culture medium after mycelia is covered with, and it is spare to be prepared into solid fungicide, and microbial inoculum spore content is 4.0 ×
108A spore/g microbial inoculum.
Inoculation totally three processing: 1) has the basket bacterium SBS-ZGTP02 solid fungicide of wart；2) cotton seed hulls solid medium blank
Control；3) sterile water blank control.
After the completion of pinus sylvestris var. mongolica vernalization, culture substrate is first handled, by phosphorus decomposing fungus solids microbial inoculum and cotton seed hulls solid medium
It is added in nutritive cube with sterile culture matrix (turfy soil: vermiculite: fine sand=2:1:1) according to the ratio of volume ratio 1:10 respectively
Afterwards, pinus sylvestris var. mongolica sowing is carried out.
Scotch pine seed through vernalization is sown into nutritive cube, 30 seeds of every alms bowl above cover 2cm thickness sterile soil, sprinkle profoundly water
After be put into greenhouse and cultivate, after coming up, final singling is to 15 plants of every alms bowl.It carries out the daily of routine to manage and protect, every 2d pours a water
(100ml/ alms bowl) pours weekly a Hoagland nutrient solution (100ml/ alms bowl).
It emerges after 90d, every processing takes 30 plants of pinus sylvestris seedlings at random, seedling growth index is measured, using ruler and trip
Mark slide calliper rule measure height of seedling and ground diameter respectively.After nursery stock sampling, rhizosphere soil is carefully cleaned up with brush, measures seedling with electronic balance
After the wooden fresh weight, the dry weight of nursery stock is weighed after 85 DEG C of drying in air dry oven.It the results are shown in Table 3 and Fig. 2.
Growth-promoting of the 3 phosphorus decomposing fungi SBS-ZGTP02 of table to pinus sylvestris seedling
Embodiment 3
It is physical and chemical to pinus sylvestris seedling rhizosphere soil to have the basket bacterium of wart (Talaromyces verruculosus) SBS-ZGTP02
Character improvement
After pinus sylvestris seedling emergence 90d in embodiment 2, each 70 plants of pinus sylvestris seedlings of processing random acquisition gently shake rhizosphere
Rhizosphere sampling is collected with sterile sampling bag, takes back laboratory with being put into the incubator equipped with ice bag after 2mm sieve,
4 DEG C of refrigerators save after natural air drying.The measurement of the measurement of soil organism potassium bichromate Outside Heating Method, full nitrogen is fixed using kelvin
The measurement of nitrogen instrument, full phosphorus are measured using molybdenum antimony resistance colorimetric method, and rapid available phosphorus is invaded using bisgallic acid and mentions molybdenum antimony resistance colorimetric method, and available potassium uses
NH4OAc, which is invaded, mentions flare photometer, and full potassium is measured using flare photometer, soil pH pH meter.Soil saccharase is living
Property, catalase activity, activity of acid phosphatase, urease activity build up kit measurement using Nanjing.It the results are shown in Table 4.
4 phosphorus decomposing fungi SBS-ZGTP02 of table improves pinus sylvestris seedling rhizosphere soil physicochemical character
As seen from the results in Table 4, phosphorus decomposing fungi SBS-ZGTP02 there is improvement to make pinus sylvestris seedling rhizosphere soil physicochemical character
With there are significant difference (P < 0.05) between different disposal, compared with blank control, add the camphor tree of phosphorus decomposing fungus solids microbial inoculum
The sub- pine stump border soil organism, full nitrogen, available nitrogen, full phosphorus, rapid available phosphorus, full potassium, available potassium be respectively increased 24.49%,
18.53%, 16.81%, 11.32%, 77.98%, 4.67% and 43.33%.Wherein rapid available phosphorus improves at most, reaches
77.98%, it was demonstrated that the addition of phosphorus decomposing fungi effectively improves pinus sylvestris var. mongolica rhizosphere soil physicochemical character, promotes the life of pinus sylvestris seedling
It is long.
Compared with blank control, after being inoculated with phosphorus decomposing fungi SBS-ZGTP02 solid fungicide, pinus sylvestris var. mongolica rhizosphere soil enzyme includes
Invertase, catalase, acid phosphatase and urease activity are respectively increased: 132.67%, 9.81%, 119.44% and
34.19%.The use of phosphorus decomposing fungal inoculant plays significant facilitation to the organized enzyme and Nutrient Cycling of rhizosphere soil.
By above embodiments, it can be concluded that, the basket bacterium of wart provided by the invention improves the dissolution of insoluble phosphate, promotes
The growth of pinus sylvestris seedling improves pinus sylvestris var. mongolica rhizosphere soil and physicochemical character, and improves the enzymatic activity in pinus sylvestris var. mongolica rhizosphere soil.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. one plant of pinus sylvestris var. mongolica rhizosphere has the basket bacterium SBS-ZGTP02 of wart, Latin is Talaromyces verruculosus, is protected
Hiding number is CGMCC No.17768.
2. the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in claim 1 is improving the application in insoluble phosphate dissolution.
3. application according to claim 2, which is characterized in that the insoluble phosphate include calcium phosphate, aluminum phosphate and
One or more of ferric phosphate.
4. the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in claim 1 is promoting the application in Seedling Growth of Mongol Scotch Pine.
5. the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart answering in the physicochemical character for improving pinus sylvestris var. mongolica rhizosphere soil described in claim 1
With.
6. application according to claim 5, which is characterized in that the physicochemical character includes the soil organism, full nitrogen, quick-acting
Nitrogen, full phosphorus, rapid available phosphorus, full potassium and available potassium.
7. the pinus sylvestris var. mongolica rhizosphere tool basket bacterium of wart described in claim 1 is improving pinus sylvestris var. mongolica according to answering in Enzyme Activities in Soils
With.
8. application according to claim 7, which is characterized in that the enzyme includes invertase, catalase, acid phosphatase
One or more of enzyme and urase.
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| CN113373069A (en) * | 2021-08-05 | 2021-09-10 | 中国科学院东北地理与农业生态研究所 | Straw saprophytic fungus and application thereof |
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| CN113373069B (en) * | 2021-08-05 | 2022-12-06 | 中国科学院东北地理与农业生态研究所 | Straw saprophytic fungus and application thereof |
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