CN1651568A

CN1651568A - Edible fungus liquid culture submerged fermentation technology

Info

Publication number: CN1651568A
Application number: CN 200410001084
Authority: CN
Inventors: 李勇; 王延林
Original assignee: Individual
Current assignee: Individual
Priority date: 2004-02-03
Filing date: 2004-02-03
Publication date: 2005-08-10

Abstract

A deeply fermenting technology for the liquid strain of edible fungus includes such steps as preparing mother strain on its culture medium, inoculating to the culture medium in shake flask, culturing, inoculating to the culture medium in seeding tank, fermenting culturing, inoculating to the culture medium in deep fermenting tank, and deeply fermenting culture.

Description

A kind of edible fungi liquid strain deep layer fermenting process

Technical field

The present invention relates to a kind of edible fungi liquid strain deep layer fermenting process, described technology uses complex medium and submerged fermentation to carry out spawn culture.

Background technology

In prior art, the production of edible fungi liquid strain has various ways, it is edible that Chinese patent 00123385.8 discloses a kind of production, the method of medicinal fungi liquid spawn, this method mainly is to utilize alcohol waste lees solution to add starch, sucrose, yeast powder is mixed with substratum, prepare the level liquid bacterial classification earlier, utilize this substratum then, adopt deep layer fermenting process, produce food, the medicinal fungi bacterial classification is realized the batch production production of bacterial classification, the pollution that had both solved grain distillery's waste discharge, the cost of producing bacterial classification is reduced greatly, bacterial classification changes the generation number and reduces, and strain quality is good, adapts to the industrialized development of edible mushrooms.Chinese patent 01114001.1 discloses a kind of edible fungi liquid strain production method, the substratum of this method is murphy juice, brown sugar, peptone, sal epsom, dipotassium hydrogen phosphate, zinc sulfate, VB1, agar etc., the incubator of this method is that the air riser tube is arranged in tank body, be air nozzle below the riser tube, Electric heating bar is arranged in the tank body.Cultivated through 3-5 days, produce qualified bacterial classification.The bacterial classification that uses aforesaid method to produce is not ideal enough.Therefore, need to propose a kind of new edible fungi liquid strain deep layer fermenting process.

Summary of the invention

The object of the present invention is to provide a kind of edible fungi liquid strain deep layer fermenting process, this zymotechnique can realize that the industrialization of liquid spawn produces fast, and bacterial classification do not have living contaminants, and strain quality is higher.

The objective of the invention is to realize: a kind of edible fungi liquid strain deep layer fermenting process by following technical proposals, using mother culture media to carry out mother's kind prepares, the mother who obtains planted implant one-level and shake in the shake-flask culture base in the bottle and carry out strain preparation, cultivate carrying out one grade fermemtation in the bacterial classification inoculation that the obtains one grade fermemtation substratum in the seeding tank, then the first class inoculum that obtains after the one grade fermemtation cultivation is inoculated in the submerged fermentation culture medium in the submerged fermentation jar and carries out the submerged fermentation cultivation, finally obtain edible fungi liquid strain;

Prepare in the described female a kind of mother of carrying out kind of selecting for use in the preparation process among mother culture media A or mother culture media B or the mother culture media C of planting;

Described mother culture media A is formed by the weight ratio allotment of following raw material:

Glucose 2

Yeast extract paste 0.5

Potassium primary phosphate 0.1

Sal epsom 0.1

Agar 2

Water 100;

Described mother culture media B is formed by the weight ratio allotment of following raw material:

Cotton seed skin fine powder 50

Semen Maydis powder 49.5

Peptone 0.5

Water 100;

Described mother culture media C is formed by the weight ratio allotment of following raw material:

Wheat bran 38

Broad-leaved wood chip 60

Glucose 1.5

Peptone 0.5

Water 100;

Described female preparation process of planting is as follows:

A, above-mentioned mother culture media raw material is accurately weighed, fully dissolving stirs, and adopts sodium hydroxide or hydrochloric acid solution to regulate PH to 6.5, is mixed with mother culture media;

B, with the described mother culture media preparation test tube of packing into, every test tube loading amount is 8～10 grams;

C, described preparation test tube is put into portable pressure kettle internal heating, keep sterilization in 30 minutes when behind steam discharge, reaching 0.108Mpa;

D, when temperature is reduced to 60 ℃, will prepare invisible spectro mother culture media and be adjusted to the inclined-plane, this chamfer length is 80～100 millimeters, is cooled to room temperature gradually;

E, employing aseptic technique, to be transplanted to after the sporophore separate tissue on the described invisible spectro mother culture media inclined-plane, cultivated 7～10 days in 25 ℃ of-26 ℃ of black outs, choose the normal most advanced and sophisticated pure mycelia of robust growth and carry out the transplanting of aseptic technique once more, should be transplanted on another invisible spectro described mother culture media inclined-plane by pure mycelia, cultivated 7-10 days through 25 ℃～27 ℃, female for the special mother kind;

In described strain preparation process, select a kind of strain preparation of carrying out among shake-flask culture base A or shake-flask culture base B or the shake-flask culture base C for use;

Described shake-flask culture base A is formed by the weight ratio allotment of following raw material:

Potato 20

Glucose 2

Peptone 0.2

Yeast powder 0.5

Potassium primary phosphate 0.1

Sal epsom 0.06

Agar 0.1

Water 100;

Described shake-flask culture base B is formed by the weight ratio allotment of following raw material:

Glucose 3

Semen Maydis powder 1

Soybean cake powder 2

Yeast powder 1

Potassium primary phosphate 0.1

Sal epsom 0.05

Agar 0.1

Water 100;

Described shake-flask culture base C is formed by the weight ratio allotment of following raw material:

Potato 20

Wheat bran 5

Peptone 0.5

Sucrose 1

Glucose 1

Potassium primary phosphate 0.2

Sal epsom 0.06

VITMAIN B1 0.01

Water 100;

Described strain preparation step is as follows:

A, by the accurate weighing of above prescription, peeling potatoes is cut into slices, Semen Maydis powder, wheat bran, soybean cake powder stir and add five times of water and be heated to 95～100 ℃ of lixiviates 30～45 minutes, getting filtrate with filtered through gauze adds other composition and constantly stirs and make its dissolving, and regulate PH to 6.5, make the shake-flask culture base;

B, the triangular flask of getting 500 milliliters of specifications shake bottle as one-level, and this shakes the described shake-flask culture base of packing in the bottle, 100 milliliters of every bottled amounts, and the triangular flask that other gets 5000 milliliters of specifications shakes bottle as secondary, and this shakes the described shake-flask culture base of packing in the bottle, 1000 milliliters of every bottled amounts;

C, with described shake the bottle place the pressure kettle internal heating, when steam discharge level pressure 0.108Mpa, keep sterilization in 30 minutes;

D, get 20 ml sterile water aseptic techniques and inject the special mother kind of described preparation test tube and smash to pieces, place in the aseptic refiner, make the homogenate bacterial classification with 5000 rev/mins of rotating speed homogenate 4 minutes;

E, 10 milliliters of aseptic techniques of described homogenate bacterial classification are inserted one-level shake bottle;

F, described one-level is shaken bottle place on the reciprocating type bottle swingging machine, under 25 ℃～27 ℃ conditions, cultivated 5～7 days with 90～100 times/minute frequency reciprocating vibrations;

G, shake the aseptic access secondary of bottle by one-level and shake bottle, described secondary is shaken bottle place on the reciprocating type bottle swingging machine, under 25 ℃～27 ℃ conditions, cultivated 5～7 days, be prepared into bacterial classification with 100～120 times/minute frequency reciprocating vibrations;

In described one grade fermemtation culturing process, select for use a kind of one grade fermemtation that carries out in one grade fermemtation culture medium A or one grade fermemtation substratum B or the one grade fermemtation culture medium C to cultivate;

Described one grade fermemtation culture medium A is formed by the weight ratio allotment of following raw material:

Glucose 2

Potato starch 2

Yeast powder 1

Milk powder 2

Peptone 0.12

Potassium primary phosphate 0.15

Sal epsom 0.01

Sodium-chlor 0.1

Zinc sulfate 0.017

Calcium chloride 0.004

Agar 0.1

Vegetables oil 0.15

VITMAIN B1 0.005

Wei ShengsuB2 0.005

Water 100;

Described one grade fermemtation substratum B is formed by the weight ratio allotment of following raw material:

Glucose 1

Peptone 0.5

Yeast powder 0.12

Potassium primary phosphate 0.15

Sal epsom 0.05

Agar 0.1

Vegetables oil 0.1

Liquid microelement 0.1

Water 100;

Described one grade fermemtation culture medium C is formed by the weight ratio allotment of following raw material:

Potato 10

Glucose 1.2

Wheat bran 4

Peptone 0.2

Sucrose 1

Potassium primary phosphate 0.15

Sal epsom 0.05

Bubble enemy 0.03

VITMAIN B1 10.01

Water 100;

Described one grade fermemtation culturing step is as follows:

Machine is cultivated in A, use submerged fermentation, checks whether each pipeline of this cultivation machine is tightly intact;

B, setting vapour pressure 0.14Mpa, live steam sterilization 2 hours, sealed valve;

C, with described one grade fermemtation substratum by prescription heating for dissolving or lixiviate, add after filtering in the one grade fermemtation jar, add water and be settled to the demarcation liquid level, sampling detects also regulates PH to 6.5;

Pressure 0.1Mpa (121 ℃ of temperature) is kept in D, thermal sterilization sterilization, after 35 minutes, and the step-down cooling;

E, one grade fermemtation jar feed sterile air, keep tank pressure 〉=0.02Mpa;

In the time of F, jar temperature drop to 28 ℃, aseptic technique inserts described secondary and shakes the bacterial classification that bottle is prepared into;

G, cultivated 36 hours down 25 ℃ of temperature, strengthen air flow and cultivated 48～60 hours, it is standby that sampling detects qualified back, finishes the one grade fermemtation of bacterial classification and cultivate;

Selecting for use a kind of among submerged fermentation culture medium A or submerged fermentation culture medium B or the submerged fermentation culture medium C to carry out submerged fermentation in described submerged fermentation culturing process cultivates;

Described submerged fermentation culture medium A is formed by the weight ratio allotment of following raw material:

Wheat bran 5

Glucose 3

Peptone 0.2

Potassium primary phosphate 0.03

Sal epsom 0.05

Vegetables oil 0.15

Water 100;

Described submerged fermentation culture medium B is formed by the weight ratio allotment of following raw material:

Semen Maydis powder 2

Yeast extract paste 0.2

Glucose 1

Potassium primary phosphate 0.1

Sal epsom 0.05

Sodium-chlor 0.05

Agar 0.1

Vegetables oil 0.15

Water 100;

Described submerged fermentation culture medium C is formed by the weight ratio allotment of following raw material:

Glucose 1.2

Potato 10

Wheat bran 4

Peptone 0.2

Sucrose 1

Potassium primary phosphate 0.15

Sal epsom 0.05

VITMAIN B1 0.01

Bubble enemy 0.03

Water 100;

Described submerged fermentation culturing step is as follows:

B, start the heating knob, vapor pressure is live steam sterilization valve-off after 2 hours during to 0.14Mpa;

C, take by weighing material by above-mentioned culture medium prescription, wherein solid material adds the lixiviate of water post-heating, crosses leaching juice, and wherein the solubility material merges in the submerged fermentation jar that the back adds the cultivation machine with hot water dissolving and described filtered juice, adds the water constant volume, adjusting PH to 6.5;

D, startup heating knob carry out the steam sterilizing sterilization, keep cooling tank pressure 0.108Mpa35 minute;

E, jar temperature drop to 28 ℃～30 ℃, close cooling water valve, open the inoculation valve, insert the bacterial classification of cultivating through one grade fermemtation with pressure differential method;

F, regulate submerged fermentation jar temperature to 26 ℃, cultivate and strengthen air flow after 36 hours and cultivated the acquisition edible fungi liquid strain 36～48 hours.

The present invention compared with prior art has following advantage:

1, because the present invention adopts liquid fermentation process and industrialized production, the production cycle of bacterial classification shortens greatly.

2, because substratum of the present invention contains many middle nutritive substances, the spawn activity of production is strong.

3, the liquid spawn of using the present invention to produce, its bacterium spherical diameter is little, and the bacterium ball density in the unit volume is big.

Embodiment

Embodiment one:

A kind of edible fungi liquid strain deep layer fermenting process, using mother culture media to carry out mother's kind prepares, the mother who obtains planted implant one-level and shake in the shake-flask culture base in the bottle and carry out strain preparation, cultivate carrying out one grade fermemtation in the bacterial classification inoculation that the obtains one grade fermemtation substratum in the seeding tank, then the first class inoculum that obtains after the one grade fermemtation cultivation is inoculated in the submerged fermentation culture medium in the submerged fermentation jar and carries out the submerged fermentation cultivation, finally obtain edible fungi liquid strain;

Glucose 2

Yeast extract paste 0.5

Potassium primary phosphate 0.1

Sal epsom 0.1

Agar 2

Water 100;

Cotton seed skin fine powder 50

Semen Maydis powder 49.5

Peptone 0.5

Water 100;

Wheat bran 38

Broad-leaved wood chip 60

Glucose 1.5

Peptone 0.5

Water 100;

Present embodiment uses mother culture media A, and described female preparation process of planting is as follows:

B, with the described mother culture media preparation test tube of packing into, this test tube diameter is 20 millimeters, every test tube loading amount is 8～10 grams, fills in tampon according to a conventional method, outsourcing kraft paper, per 10 a bundles wrapping;

C, described preparation test tube is put into portable pressure kettle internal heating, keep sterilization in 30 minutes when behind steam discharge, reaching 0.108Mpa, stop heating then, steam discharge when being depressurized to normal atmosphere naturally, and dry tampon with waste heat;

D, when temperature is reduced to 60 ℃, will prepare invisible spectro mother culture media and be adjusted to the inclined-plane, this chamfer length is 80～100 millimeters, is cooled to room temperature gradually, and prevents that water of condensation from producing;

Potato 20

Glucose 2

Peptone 0.2

Yeast powder 0.5

Potassium primary phosphate 0.1

Sal epsom 0.06

Agar 0.1

Water 100;

Glucose 3

Semen Maydis powder 1

Soybean cake powder 2

Yeast powder 1

Potassium primary phosphate 0.1

Sal epsom 0.05

Agar 0.1

Water 100;

Potato 20

Wheat bran 5

Peptone 0.5

Sucrose 1

Glucose 1

Potassium primary phosphate 0.2

Sal epsom 0.06

VITMAIN B1 0.01

Water 100;

Present embodiment uses shake-flask culture base A, and described strain preparation step is as follows:

B, the triangular flask of getting 500 milliliters of specifications shake bottle as one-level, and this shakes the described shake-flask culture base of packing in the bottle, 100 milliliters of every bottled amounts, the triangular flask that other gets 5000 milliliters of specifications shakes bottle as secondary, and this shakes the described shake-flask culture base of packing in the bottle, 1000 milliliters of every bottled amounts, tampon seals, the kraft paper wrapping;

C, the described bottle that shakes is placed the pressure kettle internal heating, when steam discharge level pressure 0.108Mpa, keep sterilization in 30 minutes, stop to heat steam discharge when being depressurized to normal atmosphere naturally then;

F, described one-level is shaken bottle place on the reciprocating type bottle swingging machine, under 25 ℃～27 ℃ conditions, cultivated 5～7 days with 90～100 times/minute frequency reciprocating vibrations, the reciprocating stroke of described bottle swingging machine is 80 millimeters;

Glucose 2

Potato starch 2

Yeast powder 1

Milk powder 2

Peptone 0.12

Potassium primary phosphate 0.15

Sal epsom 0.01

Sodium-chlor 0.1

Zinc sulfate 0.017

Calcium chloride 0.004

Agar 0.1

Vegetables oil 0.15

VITMAIN B1 0.005

Wei ShengsuB2 0.005

Water 100;

Glucose 1

Peptone 0.5

Yeast powder 0.12

Potassium primary phosphate 0.15

Sal epsom 0.05

Agar 0.1

Vegetables oil 0.1

Liquid microelement 0.1

Water 100;

Potato 10

Glucose 1.2

Wheat bran 4

Peptone 0.2

Sucrose 1

Potassium primary phosphate 0.15

Sal epsom 0.05

Bubble enemy 0.03

VITMAIN B1 0.01

Water 100;

Present embodiment adopts the one grade fermemtation culture medium A, and described one grade fermemtation culturing step is as follows:

Machine is cultivated in A, use submerged fermentation, the detailed structure of this fermentation culture machine is referring to No. 200420002455.9 utility model patent specification sheetss, or referring to No. 01114001.1 patent of invention specification sheets, do not describe in detail at this, check whether each pipeline of this cultivation machine is tightly intact;

C, with described one grade fermemtation substratum by prescription heating for dissolving or lixiviate, add after filtering in the one grade fermemtation jar (also can be described as seeding tank), add water and be settled to the demarcation liquid level, sampling detects also regulates PH to 6.5;

D, to one grade fermemtation jar thermal sterilization sterilization, keep pressure 0.1Mpa, 121 ℃ of sterilising temps, sterilization time 35 minutes, natural then step-down cooling or water cycle cooling;

E, when the one grade fermemtation tank pressure is reduced to 0.025Mpa, feed sterile air, keep tank pressure 〉=0.02Mpa;

F, when one grade fermemtation jar temperature is reduced to 28 ℃, aseptic technique inserts described secondary and shakes the bacterial classification that is prepared into of bottle;

G, adjusting air flow 1: 0.3,25 ℃ of temperature, cultivated 36 hours, air flow was cultivated 48～60 hours in 1: 0.5～1: 0.8, it is standby that sampling detects qualified back, finishing the one grade fermemtation of bacterial classification cultivates, wherein, the ratio of described air flow is meant that the tank body volume of one grade fermemtation jar and unit time enter the ratio of the volume of air of tank body;

Wheat bran 5

Glucose 3

Peptone 0.2

Potassium primary phosphate 0.03

Sal epsom 0.05

Vegetables oil 0.15

Water 100;

Semen Maydis powder 2

Yeast extract paste 0.2

Glucose 1

Potassium primary phosphate 0.1

Sal epsom 0.05

Sodium-chlor 0.05

Agar 0.1

Vegetables oil 0.15

Water 100;

Glucose 1.2

Potato 10

Wheat bran 4

Peptone 0.2

Sucrose 1

Potassium primary phosphate 0.15

Sal epsom 0.05

VITMAIN B1 0.01

Bubble enemy 0.03

Water 100;

Adopt submerged fermentation culture medium A in the present embodiment, described submerged fermentation culturing step is as follows:

A, use submerged fermentation to cultivate machine, check whether each pipeline of submerged fermentation jar of this machine is tightly intact;

B, start the heating knob, vapor pressure is during to 0.14Mpa, to submerged fermentation jar live steam sterilization valve-off after 2 hours;

C, take by weighing material by above-mentioned culture medium prescription, wherein solid material adds the lixiviate of water post-heating, 95～100 ℃ of temperature, cross leaching juice with 40～60 mesh sieves after 30～40 minutes, wherein the solubility material merges in the submerged fermentation jar of back adding cultivation machine with 70-75 ℃ of hot water dissolving and described filtered juice, add water and be settled to the demarcation liquid level, PH to 6.5 is regulated in inspection by sampling;

D, startup heating knob carry out the steam sterilizing sterilization, keep submerged fermentation jar tank pressure 0.108Mpa, 121 ℃ of temperature, and sterilization 35 minutes is opened the cooling water valve cooling then, treats to feed sterile air when tank pressure is reduced to 0.025Mpa and keep tank pressure 〉=0.02Mpa;

The jar temperature drop to 28 ℃ of E, submerged fermentation jar～30 ℃ is closed cooling water valve, opens the inoculation valve, inserts the bacterial classification of cultivating through one grade fermemtation with pressure differential method;

F, regulate submerged fermentation jar temperature to 26 ℃, tank pressure 0.02Mpa, air flow 1: 03 cube/minute; Cultivate and regulate air flow to 1 after 36 hours: cultivated 36～48 hours for 0.5～1: 0.08 cube/minute, take a sample qualified back obtains edible fungi liquid strain, wherein, the ratio of described air flow is meant that the tank body volume of one grade fermemtation jar and unit time enter the ratio of the volume of air of tank body.

The technical quality standard of present embodiment bacterial classification:

One-level is shaken a bottle bacterial classification, and nutrient solution is clear by turbid change, and color is yellowish or light brown; Has fruit aroma or almond flavor; The bacterium ball is tiny, and particulate state accounts for total amount more than 30% after static; No living contaminants; No self-dissolving or a little self-dissolving; PH≤4.5～5.5.

Secondary shakes a bottle bacterial classification, and nutrient solution is clear by turbid change, and is faint yellow or light brown; Has fruit aroma or almond flavor; Bacterium ball natural sedimentation volume is that total amount is more than 50%; Centrifugation amount 〉=20%; HP≤4.5～5.5; Microscopy does not have assorted bacterium, no self-dissolving.

One grade fermemtation jar bacterial classification, nutrient solution is as clear as crystal, is faint yellow or light brown; Has almond flavor; The mycelia particulate state, bacterium ball 〉=500/milliliter, wherein 2 millimeters bacterium balls of diameter account for 80%, evenly suspend; Centrifugation bacterium cement content 〉=20%, PH≤4～5.5, the OD:1 microscopy does not have assorted bacterium, and clamp connexion is outstanding, color depth; Incubation growth is vigorous, and it is strong to climb wall energy power, does not have assorted bacterium; Residual sugar≤2%, Iod R "-", butt output: 〉=1%.

Submerged fermentation jar bacterial classification, nutrient solution is as clear as crystal, is faint yellow to pale brown look; Has almond flavor; The mycelia particulate state, bacterium ball 〉=500/milliliter, 1～2 millimeter of bacterium spherical diameter evenly suspends; Centrifugation bacterium cement content 〉=20%; PH≤4～5.5.OD:1, microscopy does not have assorted bacterium, and clamp connexion is outstanding, color depth; Cultivate energeticly, do not have assorted bacterium; Iod R "-".Residual sugar≤2, butt 〉=1.2%.

The detection method of present embodiment:

Per 12 hours of routine sampling once; Sense index comprises color, smell, form, unit content, unit volume, output.

Detection method: color: range estimation.Smell: smell survey.

Unit content: dilution method is measured or the microscope counting method of blood cell is measured method for expressing: " individual/milliliter ".

The centrifugal 10Min of unit volume: 3000r/Min measures method for expressing " volume percent ".

Butt output: the filtering drying weighing method is measured, and method for expressing " restrains/100 milliliters ".

Physical and chemical index: comprise PH, OD, Iod R, residual sugar.

Detection method:

PH: adopt the PH instrumentation fixed.

OD: adopt 721 type spectrophotometric determinations.

Iod R: adopt iodine solution to measure.

Residual sugar: adopt hand-held saccharometer Abbe refractometer to measure.

Microbiological indicator and detection method:

Microscopy: microscopical determination, normal mycelia is transparent, and to be branch dendritic, and clamp connexion is obvious, and tabula is arranged.

The meat soup culture method: 3 of phenol red meat soup test tube substratum, dip in inoculating needle and to get the aseptic access test tube of fermented liquid, in 37 ℃ of constant temperature culture, regularly observe the substratum colour-change, as becoming faint yellowly, pipe flocks occurs in the end and is living contaminants, judges but should cultivate in conjunction with microscopy and inoculation plate.

Plate culture: under the aseptic condition, dip in and get 37 ℃ of constant temperature culture of nutrient solution streak inoculation in the meat soup plate (simultaneously 3 pairs, and do blank contrast), wait lamp detects down, its assorted bacterium form is fine hair shape or loose flocculence, and spore is printed on shades of colour, and typical plaque can appear in phage.Detect once every 6h, if assorted bacterium appears in continuous quadratic, microscopy also confirms to have assorted bacterium form, then decidable living contaminants simultaneously.

Send out the bacterium test:

Get the aseptic sample of fermentation after 36 hours, aseptic technique is inoculated in to be identified on the substratum, through 25 ℃ of constant temperature culture, is no less than 3 bottles at every turn, and normal sprouting person is judged to be normally in 12 hours.

Embodiment two:

In the present embodiment, described female the kind in the preparation process selected mother culture media B for use, select shake-flask culture base B in the described strain preparation process for use, select one grade fermemtation substratum B in the described one grade fermemtation culturing process for use, select submerged fermentation culture medium B for use in the described submerged fermentation culturing process.Other technology contents is identical with embodiment one.

Embodiment three:

In the present embodiment, described female the kind can be selected among mother culture media A, mother culture media B, the mother culture media C any one for use in the preparation process, select among shake-flask culture base A, shake-flask culture base B, the shake-flask culture base C any one in the described strain preparation process for use, select in one grade fermemtation culture medium A, one grade fermemtation substratum B, the one grade fermemtation culture medium C any one in the described one grade fermemtation culturing process for use, select among submerged fermentation culture medium A, submerged fermentation culture medium B, the submerged fermentation culture medium C any one in the described submerged fermentation culturing process for use.Other technology contents is identical with embodiment one.

Claims

1, a kind of edible fungi liquid strain deep layer fermenting process, it is characterized in that: use mother culture media to carry out mother's kind and prepare, the mother who obtains planted implant one-level and shake in the shake-flask culture base in the bottle and carry out strain preparation, cultivate carrying out one grade fermemtation in the bacterial classification inoculation that the obtains one grade fermemtation substratum in the seeding tank, then the first class inoculum that obtains after the one grade fermemtation cultivation is inoculated in the submerged fermentation culture medium in the submerged fermentation jar and carries out the submerged fermentation cultivation, finally obtain edible fungi liquid strain;

Glucose 2

Yeast extract paste 0.5

Potassium primary phosphate 0.1

Sal epsom 0.1

Agar 2

Water 100;

Cotton seed skin fine powder 50

Semen Maydis powder 49.5

Peptone 0.5

Water 100;

Wheat bran 38

Broad-leaved wood chip 60

Glucose 1.5

Peptone 0.5

Water 100;

Described female preparation process of planting is as follows:

Potato 20

Glucose 2

Peptone 0.2

Yeast powder 0.5

Potassium primary phosphate 0.1

Sal epsom 0.06

Agar 0.1

Water 100;

Glucose 3

Semen Maydis powder 1

Soybean cake powder 2

Yeast powder 1

Potassium primary phosphate 0.1

Sal epsom 0.05

Agar 0.1

Water 100;

Potato 20

Wheat bran 5

Peptone 0.5

Sucrose 1

Glucose 1

Potassium primary phosphate 0.2

Sal epsom 0.06

VITMAIN B1 0.01

Water 100;

Described strain preparation step is as follows:

Glucose 2

Potato starch 2

Yeast powder 1

Milk powder 2

Peptone 0.12

Potassium primary phosphate 0.15

Sal epsom 0.01

Sodium-chlor 0.1

Zinc sulfate 0.017

Calcium chloride 0.004

Agar 0.1

Vegetables oil 0.15

VITMAIN B1 0.005

Wei ShengsuB2 0.005

Water 100;

Glucose 1

Peptone 0.5

Yeast powder 0.12

Potassium primary phosphate 0.15

Sal epsom 0.05

Agar 0.1

Vegetables oil 0.1

Liquid microelement 0.1

Water 100;

Potato 10

Glucose 1.2

Wheat bran 4

Peptone 0.2

Sucrose 1

Potassium primary phosphate 0.15

Sal epsom 0.05

Bubble enemy 0.03

VITMAIN B1 0.01

Water 100;

Described one grade fermemtation culturing step is as follows:

E, one grade fermemtation jar feed sterile air, keep tank pressure 〉=0.02Mpa;

Wheat bran 5

Glucose 3

Peptone 0.2

Potassium primary phosphate 0.03

Sal epsom 0.05

Vegetables oil 0.15

Water 100;

Semen Maydis powder 2

Yeast extract paste 0.2

Glucose 1

Potassium primary phosphate 0.1

Sal epsom 0.05

Sodium-chlor 0.05

Agar 0.1

Vegetables oil 0.15

Water 100;

Glucose 1.2

Potato 10

Wheat bran 4

Peptone 0.2

Sucrose 1

Potassium primary phosphate 0.15

Sal epsom 0.05

VITMAIN B1 0.01

Bubble enemy 0.03

Water 100;

Described submerged fermentation culturing step is as follows: