CN100370015C - Culture for caterpillar and cordycepin - Google Patents

Abstract

The present invention provides a method for culturing aweto fungus and Chinese caterpillar fungus, which is characterized in that a culture medium is prepared from the components of the following proportion by mass: 1% to 1.5% of milk powder, 2% to 2.5% of soybean flour, 2% to 4% of corn flour, 2% to 4% of wheat flour, 1% to 2% of glucose, 1% to 2% of sucrose, 0.05% to 0.08% of potassium dihydrogen phosphate, 0.05% to 0.2% of defoaming agent and 85% to 88% of water; the components are boiled into paste and put into a bottle, and after the paste is autoclaved, the paste is inoculated; the paste is cultured for 22 to 32 days at the temperature of 10 DEG C to 30 DEG C, so that mycelium which is crisp, thick, fragrant, delicious and rich in nutrition is obtained, can completely make up for the defects of natural products; not only various health products and medicines with high nutrient value and strong medicinal efficiency can be prepared by using the present invention, but also the diversity and the uniqueness of eating the present invention can be fully embodied. The culture medium provided by the present invention has a scientific and reasonable formula, and can enable the nutrient components of the culture medium to be distributed more uniformly after the culture medium is cooked, so that strains are easy to grow, the requirement of the culture process is not high and strict, the difficulty of the culture process is not large, and the culture medium is favorable for industrialized production and scale production.

Description

The cultural method of Cordyceps fungus or Cordyceps militaris (L.) Link. fungus

Technical field

The present invention relates to a kind of cultural method of biological bacteria, especially the cultural method of Cordyceps fungus, Cordyceps militaris (L.) Link. fungus belongs to biological technical field.

Background technology

Cordyceps sinensis is All Pure Nature high-grade nourishing nutritious prod and the rare traditional Chinese medicine that people generally acknowledge.Because of it is subjected to the influence of natural growthing condition bigger, makes the natural product limited amount, and cost an arm and a leg.For this reason, people's demand be satisfied, the artificial culture technology must be greatly developed.Cordyceps fungus, Cordyceps militaris (L.) Link. fungus are Mycophyta, belong to Cordyceps, and its mycelium is the vegetative organ of dietotherapeutic fungi, also are the main bodys of dietotherapeutic fungi.Last century the seventies, develop meticulously through domestic expert, can from natural cs, isolate multiple bacterial classification, use the submerged fermentation technology and turn out Cordyceps mycelium, and be that raw material is allocated general excipient substance into this tunning, make various medicinal preparationss and healthcare products.But the product that adopts the submerged fermentation technology to obtain can not keep original local flavor of its sporophore and quality, and off-odor is arranged, and can not directly eat, and the equipment used of submerged fermentation technology in addition is huge, technical sophistication, thus limited production and the development of this class fungi.Therefore, be necessary prior art is improved.

Summary of the invention

For overcoming yielding poorly that prior art exists, ingredient is impure, and local flavor and quality are not good to wait deficiency, the invention provides a kind of suitable for mass production, and the cultural method of the Cordyceps fungus of quality better, Cordyceps militaris (L.) Link. fungus.

The present invention realizes by following technical proposal: the cultural method of a kind of Cordyceps fungus or Cordyceps militaris (L.) Link. fungus is characterized in that comprising the following steps:

A, substratum

A, the following quality proportioning of employing: milk powder 1～1.5%, analysis for soybean powder 2～2.5%, Semen Maydis powder 2～4%, flour 2～4%, glucose 1～2%, sucrose 1～2%, potassium primary phosphate 0.05～0.08%, defoamer 0.05～0.2%, water 85～88%;

B, through following technology: after above-mentioned raw materials mixed, in whipping process, boil into scattered paste shape;

B, cultivation

A, the scattered paste shape substratum that the A step is obtained are packed in the culturing bottle, behind the sealing bottleneck, are under 0.12～0.2Mpa at pressure, and steam sterilizing 30～40 minutes treats that pressure reduces to zero, when temperature is reduced to below 60 ℃, takes out culturing bottle;

B, the amount of pressing every 100ml inoculation 2～4ml are injected liquid spawn in the bottle that substratum is housed, and under 10～30 ℃ of temperature, cultivate 22～32 days, get thalline;

C, thalline is put into 60～75 ℃ of warm water soaked 3～8 minutes, clean with clear water afterwards, promptly get bright product.

Described defoamer is a soybean oil.

Described milk powder is milk powder.

The present invention has following advantage and effect: adopt such scheme; it is thick to obtain the crisp meat of matter; flavor is fragrant good to eat; the thalline that nutritive substance is abundant; can compare favourably with natural sporophore; well sold and in short supply at natural cs; output is very limited; expensive current society; be undoubtedly a kind of preferred substitute; can remedy the deficiency of natural product fully; not only can be made into the various healthcare products that are of high nutritive value with it; the medicine that medicinal efficacy is strong; can also demonstrate fully diversity and uniqueness when eating; cultural method provided by the invention; remove that to have equipment simple, invest for a short time, raw material is easy to get; the output height; production link is common, and is easy to control, easy to operate; be not subject to seasonal restrictions etc. outside the characteristics; the main not only scientific formulation of substratum that also is; rationally, and more even through its nutritive ingredient is distributed, the very easily growth of bacterial classification; in the whole culturing process; culture condition such as temperature; humidity etc. are less demanding; not tight, difficulty is little, therefore; help industrialization; large-scale production is the good yeast culture technology of an ideal popularizing application prospect in fact.

Following table provides with the Cordyceps fungus of the present invention's method acquisition and the aminoacids content measurement result of Cordyceps militaris (L.) Link. fungus.

Sequence number		Title	Cordyceps sinensis (mg/g)	Cordyceps militaris (L.) Link. (mg/g)
					1	ASP	Aspartic acid	0.11	1.07
2	GLU	L-glutamic acid	0.160	1.18
					3	SER	Serine	0.26
4	GLY	Glycine		0.64
					5	HIS	Histidine	0.15	1.58
6	ARG	Arginine	0.19	0.32
					7	THR	Threonine	0.13	0.33
8	ALA	L-Ala	0.21	0.56
					9	PRO	Proline(Pro)	0.91	1.90
10	TYR	Tyrosine	0.07	0.04
					11	VAL	Xie Ansuan	0.13	0.36
12	MET	Methionine(Met)	0.05	0.07
					13	CYS	Cysteine	0.06	0.16
14	ILE	Isoleucine	0.08	0.12
					15	LEU	Leucine	0.09	0.20
16	PHE	Phenylalanine	0.06	0.07
					17	LYS	Methionin	0.05	0.18

Embodiment

Embodiment 1

1, takes off the row raw material: milk powder 1kg, analysis for soybean powder 2.5kg, Semen Maydis powder 3kg, flour 3.3kg, glucose 2kg, sucrose 1kg, potassium primary phosphate 0.08kg, soybean oil 0.12kg, water 87kg;

2, through following technology: soybean oil is gone into pot smear, milk powder, analysis for soybean powder, Semen Maydis powder, flour, glucose, sucrose, potassium primary phosphate are put into container to be mixed, be in harmonious proportion evenly with small amount of water, add enough water afterwards, go into pot behind the stirring and evenly mixing, in whipping process, boil the scattered paste shape substratum;

3, scattered paste shape substratum branch is packed into bottle, every bottled 100ml with poly-third plastics film sealing bottleneck, puts into high-pressure steam sterilizing pan, under 0.12Mpa, high pressure steam sterilization 40 minutes treats that pressure reduces to zero naturally, when temperature drops to 59 ℃, take out, put into transfer room;

4, with the syringe of the bacterium of going out Cordyceps sinensis liquid spawn 2ml is injected bottled substratum after the above-mentioned sterilization, behind medical proof fabric sealing pin hole, put into the culturing room of ultraviolet lamp sterilization;

5, under 10 ℃ of temperature, cultivated 32 days, get thalline;

6, thalline is put into 60 ℃ of warm water and soaked 8 minutes, clean with clear water afterwards, promptly getting thickness is the bright product of Cordyceps fungus of 10mm.

Embodiment 2

1, takes off the row raw material: milk powder 1.5kg, analysis for soybean powder 2.3kg, Semen Maydis powder 4kg, flour 3kg, glucose 2kg, sucrose 2kg, potassium primary phosphate 0.05kg, soybean oil 0.15, water 85kg;

2, through following technology: soybean oil is gone into pot smear, milk powder, analysis for soybean powder, Semen Maydis powder, flour, glucose, sucrose, potassium primary phosphate are put into container to be mixed, be in harmonious proportion evenly with small amount of water, add enough water afterwards, go into pot behind the stirring and evenly mixing, in whipping process, boil the scattered paste shape substratum;

3, scattered paste shape substratum branch is packed into bottle, every bottled 100ml with poly-third plastics film sealing bottleneck, puts into high-pressure steam sterilizing pan, under 0.2Mpa, high pressure steam sterilization 30 minutes treats that pressure reduces to zero naturally, when temperature drops to 40 ℃, take out, put into transfer room;

4, with the syringe of the bacterium of going out Cordyceps militaris (L.) Link. liquid spawn 4ml is injected bottled substratum after the above-mentioned sterilization, behind medical proof fabric sealing pin hole, put into the culturing room of ultraviolet lamp sterilization;

5, under 30 ℃ of temperature, cultivated 22 days, get thalline;

6, thalline is put into 75 ℃ of warm water and soaked 3 minutes, clean with clear water afterwards, promptly getting thickness is the bright product of Cordyceps militaris (L.) Link. fungus of 12mm.

Embodiment 3

1, takes off the row raw material: milk powder 1.2kg, analysis for soybean powder 2.2kg, Semen Maydis powder 2.5kg, flour 2.5kg, glucose 1.5kg, sucrose 1.9kg, potassium primary phosphate 0.06kg, soybean oil 0.14kg, water 88kg;

2, through following technology: soybean oil is gone into pot smear, milk powder, analysis for soybean powder, Semen Maydis powder, flour, glucose, sucrose, potassium primary phosphate are put into container to be mixed, be in harmonious proportion evenly with small amount of water, add enough water afterwards, go into pot behind the stirring and evenly mixing, in whipping process, boil the scattered paste shape substratum;

3, scattered paste shape substratum branch is packed into bottle, every bottled 100ml with poly-third plastics film sealing bottleneck, puts into high-pressure steam sterilizing pan, under 0.15Mpa, high pressure steam sterilization 35 minutes treats that pressure reduces to zero naturally, when temperature drops to 50 ℃, take out, put into transfer room;

4, with the syringe of the bacterium of going out Cordyceps sinensis liquid spawn 3ml is injected bottled substratum after the above-mentioned sterilization, behind medical proof fabric sealing pin hole, put into the culturing room of ultraviolet lamp sterilization;

5, under 20 ℃ of temperature, cultivated 28 days, get thalline;

6, thalline is put into 70 ℃ of warm water and soaked 5 minutes, clean with clear water afterwards, promptly getting thickness is the bright product of Cordyceps fungus of 15mm.

Claims (3)

1. the cultural method of Cordyceps fungus or Cordyceps militaris (L.) Link. fungus is characterized in that comprising the following steps:

A, substratum

A, the following quality proportioning of employing: milk powder 1～1.5%, analysis for soybean powder 2～2.5%, Semen Maydis powder 2～4%, flour 2～4%, glucose 1～2%, sucrose 1～2%, potassium primary phosphate 0.05～0.08%, defoamer 0.05～0.2%, water 85～88%;

B, through following technology: after above-mentioned raw materials mixed, in whipping process, boil into scattered paste shape;

B, cultivation

A, the scattered paste shape substratum that the A step is obtained are packed in the culturing bottle, behind the sealing bottleneck, are under 0.12～0.2Mpa at pressure, and steam sterilizing 30～40 minutes treats that pressure reduces to zero, when temperature is reduced to below 60 ℃, takes out culturing bottle;

B, the amount of pressing every 100ml inoculation 2～4ml are injected liquid spawn in the bottle that substratum is housed, and under 10～30 ℃ of temperature, cultivate 22～32 days, get thalline;

C, thalline is put into 60～75 ℃ of warm water soaked 3～8 minutes, clean with clear water afterwards, promptly get bright product.

2. the cultural method of Cordyceps fungus according to claim 1 or Cordyceps militaris (L.) Link. fungus is characterized in that described defoamer is a soybean oil.

3. the cultural method of Cordyceps fungus according to claim 1 or Cordyceps militaris (L.) Link. fungus is characterized in that described milk powder is milk powder.