CN104823716A - Culture and preparing method of fungus symbiotic hypha powder - Google Patents

Culture and preparing method of fungus symbiotic hypha powder Download PDF

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Publication number
CN104823716A
CN104823716A CN201510229973.7A CN201510229973A CN104823716A CN 104823716 A CN104823716 A CN 104823716A CN 201510229973 A CN201510229973 A CN 201510229973A CN 104823716 A CN104823716 A CN 104823716A
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cultivation
mycelia
hypha
symbiosis
culture
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CN201510229973.7A
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郭亚涛
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郭亚涛
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Abstract

The invention provides a culture and preparing method of fungus symbiotic hypha powder. The method comprises the following steps of mother seed separation, hypha activation culture, hypha primary culture, hypha purification culture, hypha liquid symbiotic culture, hypha symbiotic enlarged culture, drying and crushing. Various fungi can be simultaneously cultured under the same culture condition to generate hypha; the hypha is made into corresponding hypha powder; the requirements of the market on fungus products are met. The culture and preparing method has the advantages that the manufacturing process is simple, the operation is easy, wild cordyceps sinensis, matsutake and the like can be subjected to hypha factory production, and can be made into fast thawing instant hypha powder, and the quality and the yield of various kinds of rare hypha powder can be improved.

Description

A kind of cultivation of mushroom symbiosis hypha powder and preparation method
Technical field
The invention belongs to mushroom symbiosis and cultivate field, specifically a kind of cultivation of mushroom symbiosis hypha powder and preparation method.
Background technology
In numerous fungi plant, have and much all there is high medical value, and remaining most kind all can be used as food materials uses, the mushroom with medical value not only has beneficial gas, keep fit, get rid of illness, stimulate the menstrual flow, the function such as lengthen one's life, also tool strengthens body immunity, antitumor effect such as anticancer simultaneously, mushroom nutritive value as food materials is also very high, containing more protein, carbohydrate, vitamin and trace element, eat more and can strengthen body immunity, therefore, some mushrooms Chang Zuowei health products use.
Traditional mushroom mostly is and grows in the wild; artificial searching is also won; although wild mushroom nutrient component and medical value all very high; but because few, the poky reason of its quantity causes price also higher; in order to meet the need of market; now the cultural method of the existing becteriums product such as Artificial Cultured Cordyceps Sinensis, matsutake is succeeded in developing, but all cannot accomplish scale production, and still cannot meet the demand in market.
Now commercially sold hypha powder great majority are single a kind of mushrooms, or with Chinese herbal medicine with the use of, the reason that bacterial classification is single will inevitably cause the problem of poor effect, and will take to the ill with the words of drug matching, can not act on common health products to use.
Summary of the invention
The invention provides a kind of cultivation and preparation method of mushroom symbiosis hypha powder, it can make multiple mushroom co-incubation under identical condition of culture generate mycelia, and makes corresponding hypha powder, meets the demand of market to becteriums product.
The present invention realizes above-mentioned purpose by the following technical solutions:
The cultivation of mushroom symbiosis hypha powder and a preparation method, it comprises the following steps: the separation of mother's kind, mycelia activation culture, the elementary cultivation of mycelia, the cultivation of mycelia purifying, mycelia liquid symbiosis cultivation, mycelia symbiosis expand cultivation, oven dry, pulverizing.
It is the raw material adding following quality in every premium on currency that the elementary cultivation of described mycelia and purifying cultivate agar medium used: wheat 100g, potato 100g, glucose 20g, agar 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 005g, peptone 2g, beef extract 0.5g, vitamin B1 10mg.
The elementary cultivation of described mycelia, mycelia purifying incubation time are respectively 6-10 days, and cultivation temperature is 18-25 DEG C, and cultivation humidity is 60-70%rh.
Raw material and the preparation method of described mycelia symbiosis liquid nutrient medium are: get pine needle, potato, Ipomoea batatas, bean sprouts, wheat bran, each 100-200g of fig, then add 2000ml poach taking juice, then in the above-mentioned juice of every 2000ml, add glucose 20g, vitamin B1 20mg, cobalamin 20mg, potassium dihydrogen phosphate 2g, magnesium sulfate 2g, peptone 2g, beef extract 1g, yeast extract 1g.
The time that described mycelia liquid symbiosis is cultivated is 4-7 days, and cultivation temperature is 22-25 DEG C.
The proportion by weight that described mycelia symbiosis expands the culture matrix needed raw material cultivated is as follows: 85 parts, grain, matrimony vine 12 parts, edible plaster 1 part, 2 parts, lime, 98 parts, water.
The proportion by weight of the raw material contained by described grain is as follows: wheat 55 parts, 10 parts, black soya bean, soya bean 10 parts, 10 parts, red bean.
The preparation method that described mycelia symbiosis expands the culture matrix cultivated is:
1) take corresponding water and add lime, for subsequent use after stirring;
2) grain taking respective amount adds in above-mentioned limewash to soak after 8 hours to be pulled out, and is rinsed well with clear water;
3) by step 2) in the grain of wash clean add poach 15-25 minute of 1.5 times of weight after pull out dry for subsequent use;
4) take corresponding matrimony vine and rinse well with clear water for subsequent use;
5) take the gypsum of respective amount jointly to mix with the matrimony vine in the grain in step 3) and step 4), moisture 45%-60%, packing 850ml blake bottle mesohigh, 121 degree of lower sterilizing 1.5-3 hour.
The present invention adopts technique scheme to have following beneficial effect:
Manufacture craft is simple, easily operates, by mycelia factorial praluction such as wild Cordyceps sinensis, matsutakes, can make the hypha powder of instant, improve the Quality and yield of various rare mushroom hypha powder.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and the present embodiment, carries out cultivating and obtaining hypha powder for Object of Development with Cordyceps sinensis, matsutake, Sparassis crispa, hickory chick four kinds of mushrooms under medium of the present invention and condition of culture.
Concrete operation step is as follows:
1, female kind is separated
First first each instrument is used ultraviolet lamp sterilizing 30 minutes in aseptic inoculation room.
Then get Cordyceps sinensis, matsutake, Sparassis crispa, hickory chick standard field non-hibernating eggs mushroom in aseptic inoculation indoor, the surface of the various mushroom of the alcohol wipe with 75% carries out disinfection to it, and blots with sterile gauze, is placed in plane incubator for subsequent use.
With sterilized vaccinating lancet, Cordyceps sinensis, matsutake, Sparassis crispa and hickory chick kind mushroom are cut, cut a thin slice as inoculation block at the central part of various mushroom.
Put into agar medium is housed in vitro with transfer needle picking inoculation block, finish writing label, cultivation 7-10 days in the culturing room being placed in 22-28 DEG C, mycelia begins to extend growth, takes out for subsequent use.
2, mycelia activation culture
By the mycelium of the mycelia of gained in step 1 in the indoor picking stalwartness of aseptic inoculation, size is 0.1 × 0.1cm, is placed in new agar medium matter test tube respectively, labelledly at 22-28 DEG C, cultivates 10-15 days, takes out for subsequent use after being bred as larger mycelia.
3, the elementary cultivation of mycelia
First, the medium of elementary cultivation and purifying cultivation is produced according to following ratio.
The raw material of following quality is added: wheat 100g, potato 100g, glucose 20g, agar 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 005g, peptone 2g, beef extract 0.5g, vitamin B1 10mg in every 1000ml water.
Indoor through ultraviolet sterilization 30 minutes at aseptic inoculation by being equipped with the test tube of agar medium and inoculating tool, then the mycelia in step 2 is got 0.2 × 0.2cm size mycelia to be placed in respectively and agar medium to be housed in vitro, cultivate 7-10 days, obtain one-level mycelia for subsequent use.
4, mycelia purifying is cultivated
In aseptic inoculation indoor, the one-level mycelia of gained in step 3 is got 0.2 × 0.2cm size mycelia, be placed in the new test tube that agar medium is housed respectively and carry out purifying cultivation, incubation time is 7 days, obtains the mycelia of purifying.
5, the symbiosis of mycelia liquid is cultivated
Produce culture fluid according to following proportioning: pine needle, potato, Ipomoea batatas, bean sprouts, wheat bran, fig limb boil the juice got, glucose 2g, vitamin B1 10mg, cobalamin 10mg, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, peptone 0.2g, beef extract 0.1g, yeast extract 0.1g.
Be dispensed into by liquid medium in 500ml triangular flask, every triangular flask is in-built gets 150ml liquid medium, and in every triangular flask, place 3-5 grain diameter be the bead of 1cm, and then at 121 DEG C, sterilizing is taken out for subsequent use after 45 minutes.
By indoor through ultraviolet disinfection at aseptic inoculation to triangular flask and inoculating tool that liquid medium is housed, the purifying mycelia of getting in step 4 is inserted in triangular flask, the size of purifying mycelia is 1 × 1cm, quiescent culture 24 hours at 18-22 DEG C, then go up shaking table and cultivate 3-6 days at 22-26 DEG C, obtain elementary symbiosis mycelia liquid.
Elementary symbiosis mycelia liquid to be loaded in the fermentation tank filling new liquid medium according to the ratio of mass parts 3-5% and cultivate, incubation time is 4-7 days, and cultivation temperature is 22-28 degree, obtains ultimate symbiosis mycelia liquid.
6, mycelia symbiosis expands cultivation
The proportion by weight that mycelia symbiosis expands the culture matrix needed raw material cultivated is as follows: 85 parts, grain, matrimony vine 12 parts, edible plaster 1 part, 2 parts, lime, 98 parts, water.
The proportion by weight of the raw material contained by grain is as follows: wheat 55 parts, 10 parts, black soya bean, soya bean 10 parts, 10 parts, red bean.
The preparation method that mycelia symbiosis expands the culture matrix cultivated is:
1) take corresponding water and add lime, for subsequent use after stirring;
2) grain taking respective amount adds in above-mentioned limewash to soak after 8 hours to be pulled out, and is rinsed well with clear water;
3) by step 2) in the grain of the wash clean poach that adds 1.5 times of weight pull out after 20 minutes dry for subsequent use;
4) take corresponding matrimony vine and rinse well with clear water for subsequent use;
5) take the gypsum of respective amount jointly to mix with the matrimony vine in the grain in step 3) and step 4), control moisture at 45%-60%, packing 850ml blake bottle mesohigh, 121 degree of lower sterilizings 2 hours, take out equitemperature and be down to about 30 DEG C can to insert aseptic inoculation room etc. to be seeded.
Get the ultimate symbiosis mycelia liquid 40ml of gained in step 5 and inject blake bottle, at 26 DEG C, cultivate 20-25 days, mycelia is taken out after covering with bottle.
7, dry
The particle mycelia of step 6 gained is placed in 65-75 degree warm air drier, dries 20 hours.
8, pulverize
Described symbiosis hypha powder is pulverized and obtained to the particle mycelia of drying in step 7.
The present embodiment only carries out aid illustration to technical solutions according to the invention, does not limit concrete particular content.

Claims (8)

1. the cultivation of mushroom symbiosis hypha powder and a preparation method, it comprises the following steps: femalely plant separations, mycelia activation culture, the elementary cultivation of mycelia, mycelia purifying cultivated, the symbiosis of mycelia liquid is cultivated, mycelia symbiosis expand cultivate, dry, pulverizing.
2. the cultivation of a kind of mushroom symbiosis hypha powder according to claim 1 and preparation method, is characterized in that: it is the raw material adding following quality in every premium on currency that the elementary cultivation of described mycelia and purifying cultivate agar medium used: wheat 100g, potato 100g, glucose 20g, agar 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, peptone 2g, beef extract 0.5g, vitamin B1 10mg.
3. the cultivation of a kind of mushroom symbiosis hypha powder according to claim 1 and 2 and preparation method, is characterized in that: the elementary cultivation of described mycelia, mycelia purifying incubation time are respectively 6-10 days, and cultivation temperature is 18-25 DEG C, and cultivation humidity is 60-70%rh.
4. the cultivation of a kind of mushroom symbiosis hypha powder according to claim 1 and preparation method, it is characterized in that: raw material and the preparation method of described mycelia symbiosis liquid nutrient medium are: get pine needle, potato, Ipomoea batatas, bean sprouts, wheat bran, each 100-200g of fig, then add 2000ml poach taking juice, then in the above-mentioned juice of every 2000ml, add glucose 20g, vitamin B1 20mg, cobalamin 20mg, potassium dihydrogen phosphate 2g, magnesium sulfate 2g, peptone 2g, beef extract 1g, yeast extract 1g.
5. the cultivation of a kind of mushroom symbiosis hypha powder according to claim 1 or 4 and preparation method, is characterized in that: the time that described mycelia liquid symbiosis is cultivated is 4-7 days, and cultivation temperature is 22-25 DEG C.
6. the cultivation of a kind of mushroom symbiosis hypha powder according to claim 1 and preparation method, is characterized in that: the proportion by weight that described mycelia symbiosis expands the culture matrix needed raw material cultivated is as follows: 85 parts, grain, matrimony vine 12 parts, edible plaster 1 part, 2 parts, lime, 98 parts, water.
7. the cultivation of a kind of mushroom symbiosis hypha powder according to claim 6 and preparation method, is characterized in that: the proportion by weight of the raw material contained by described grain is as follows: wheat 55 parts, 10 parts, black soya bean, soya bean 10 parts, 10 parts, red bean.
8. the cultivation of a kind of mushroom symbiosis hypha powder according to claim 6 and preparation method, is characterized in that: the preparation method that described mycelia symbiosis expands the culture matrix cultivated is:
1) take corresponding water and add lime, for subsequent use after stirring;
2) grain taking respective amount adds in above-mentioned limewash to soak after 8 hours to be pulled out, and is rinsed well with clear water;
3) by step 2) in the grain of wash clean add poach 15-25 minute of 1.5 times of weight after pull out dry for subsequent use;
4) take corresponding matrimony vine and rinse well with clear water for subsequent use;
5) take the gypsum of respective amount jointly to mix with the matrimony vine in the grain in step 3) and step 4), moisture 45%-60%, packing 850ml blake bottle mesohigh, 121 degree of lower sterilizing 1.5-3 hour.
CN201510229973.7A 2015-05-08 2015-05-08 Culture and preparing method of fungus symbiotic hypha powder Pending CN104823716A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106148198A (en) * 2016-07-05 2016-11-23 青海珠峰冬虫夏草工程技术研究有限公司 A kind of Cordyceps fluid medium and preparation method thereof
CN106212042A (en) * 2016-07-04 2016-12-14 李业武 A kind of pseudo-wild cultivating method of Tricholoma matsutake (lto et lmai) Singer
CN108929858A (en) * 2017-05-26 2018-12-04 周丽 A kind of culture and preparation method of mushroom symbiosis hypha powder
CN110122181A (en) * 2019-03-12 2019-08-16 李航 A method of Sparassis crispa bacterium grain is produced with quinoa

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1181901A (en) * 1997-11-03 1998-05-20 黄少云 Preparation process and product of nutritive glossy ganoderma liquid
CN1208575A (en) * 1998-07-21 1999-02-24 李健 Method for preparing health-care food contg. Chinese caterpillar fungus
CN102835254A (en) * 2012-09-26 2012-12-26 烟台龙宇灵芝生物开发有限公司 Ganoderma lucidum mycelia powder production process
CN103340396A (en) * 2013-07-25 2013-10-09 孟凡格 Method for producing edible and medicinal fungus powder
CN103392502A (en) * 2013-06-20 2013-11-20 姚大军 Preparation method of toadstool mycelium powder

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1181901A (en) * 1997-11-03 1998-05-20 黄少云 Preparation process and product of nutritive glossy ganoderma liquid
CN1208575A (en) * 1998-07-21 1999-02-24 李健 Method for preparing health-care food contg. Chinese caterpillar fungus
CN102835254A (en) * 2012-09-26 2012-12-26 烟台龙宇灵芝生物开发有限公司 Ganoderma lucidum mycelia powder production process
CN103392502A (en) * 2013-06-20 2013-11-20 姚大军 Preparation method of toadstool mycelium powder
CN103340396A (en) * 2013-07-25 2013-10-09 孟凡格 Method for producing edible and medicinal fungus powder

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106212042A (en) * 2016-07-04 2016-12-14 李业武 A kind of pseudo-wild cultivating method of Tricholoma matsutake (lto et lmai) Singer
CN106148198A (en) * 2016-07-05 2016-11-23 青海珠峰冬虫夏草工程技术研究有限公司 A kind of Cordyceps fluid medium and preparation method thereof
CN106148198B (en) * 2016-07-05 2019-12-13 青海珠峰冬虫夏草工程技术研究有限公司 Cordyceps sinensis liquid culture medium and preparation method thereof
CN108929858A (en) * 2017-05-26 2018-12-04 周丽 A kind of culture and preparation method of mushroom symbiosis hypha powder
CN110122181A (en) * 2019-03-12 2019-08-16 李航 A method of Sparassis crispa bacterium grain is produced with quinoa

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Application publication date: 20150812