CN104186204A - Quality inspection method for edible fungus raw materials - Google Patents
Quality inspection method for edible fungus raw materials Download PDFInfo
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- CN104186204A CN104186204A CN201410469334.3A CN201410469334A CN104186204A CN 104186204 A CN104186204 A CN 104186204A CN 201410469334 A CN201410469334 A CN 201410469334A CN 104186204 A CN104186204 A CN 104186204A
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Abstract
The invention discloses a quality inspection method for edible fungus raw materials. The method includes the following steps of raw material pretreatment; raw material hot water extraction; culture medium preparation, inspection, sterilization and cooling; inoculation, cultivation and comprehensive quality evaluation. By the quality inspection method for the edible fungus raw materials, influences of the raw materials on strain and hypha production can be reflected more accurately, and inspection is performed from physiological property so as to evaluate the quality of the raw materials.
Description
Technical field
The present invention relates to edible fungus industrial production field, relate to specifically a kind of method for quickly detecting of edible fungus industrial production raw material quality.
Background technology
Edible fungus industrial is produced raw material used and in edible fungus industrial is produced, is played the important function of prerequisite property, and that its quality stable produced edible fungus industrial is stable, high yield, high-quality etc. all affect huge.But the destabilizing factor of raw material quality is more, be the unstable of supplier (place of production) on the one hand; Be raw material quality on the other hand with the variation in season, the raw material quality gap in the different places of production of Various Seasonal is larger.
At present, all edible mushroom enterprises are all to adopt simple inspection method to carry out inspection of quality to the inspection of raw and process materials, its crucial control criterion is as organoleptic indicator's (color, smell), granularity, pH value, water content, these indexs are merely able to meet the technological requirement of producing early stage, and the mycelial growth of the edible mushroom that raw material are produced batch production impact, the impacts of the yield and quality on fruiting cultivation etc., all cannot judge.In addition, the edible fungus industrial production cycle is longer, as the true Ji mushroom cycle of general factory culture take 100 ?120 days, Asparagus, Xingbao mushroom take 50 ?60 days, after one-period finishes, can truly reflect the impact of raw material quality on this edible mushroom, during this, these raw material have used a lot of batches, if quality generation problem, to affect for a long time production, lose huge.
Summary of the invention
The method of inspection that the invention provides a kind of edible mushroom raw material quality, it comprises the steps:
Pre-treatment of raw material; The lixiviate of raw material hot water; Preparation inspection medium, sterilizing, cooling; Inoculation, cultivation, quality comprehensive are passed judgment on;
Specifically, the method for inspection of a kind of edible mushroom raw material quality of the present invention, comprises the steps:
(1) pre-treatment of raw material
Raw material are pulverized, crossed 10～40 order sieves;
(2) raw material hot water lixiviate
Raw material after pulverizing and sieving are put into water lixiviate, and material-water ratio (mass ratio) is 0.2～0.3:1, water temperature be 60 ?85 DEG C, extraction time be 25 ?30min, get leachate for subsequent use;
(3) preparation inspection medium, sterilizing, cooling
Inspection culture medium prescription be PDA powder and culture medium solution, wherein the weight percent content of PDA powder be 2 ?4%, wherein in culture medium solution, comprise raw material leachate and distilled water, leachate: distilled water (volume ratio)=0.6～1.0:1;
(4) inoculation, cultivation, quality comprehensive are passed judgment on
Detect when operation, add do a PDA medium contrast (medium contrast is made up of PDA powder and water, the weight percent content of PDA powder be 2 ?4%.) process, testing result is compared with contrasting, according to mycelial growth rate, mycelial growth potential, the uniformity, judge raw material quality quality.
According to inspection raw material disposal mycelia to compare relevant performance assessment criteria, synthetic determination raw material quality.Under normal circumstances, if mycelial growth potential is processed in inspection and growth rate is all better than or is synchronized with control treatment, illustrate that this raw material quality is good, can be used in large production; If it is not to be all better than control treatment that mycelial growth potential and growth rate are processed in inspection, illustrate that this raw material quality is general, comprehensively other organoleptic indicators and water content, pH etc. judge its quality, do not check and accept at first for the production of; If mycelial growth potential is processed in inspection and growth rate is all obviously not so good as control treatment, can judge that this raw material quality is not good or be not suitable for the large production and application for true Ji mushroom.
Innovation of the present invention is:
(1) compared with traditional edible mushroom raw material Rapid examination method, the present invention can react raw material more exactly on producing the impact of bacterial classification mycelia, tests from physiological property, is the basic standards of judging raw material quality;
(2) edible mushroom inspection of raw and process materials method of the present invention 7 ?within 14 days, can draw the good and bad result of raw material quality, conventional method need to be passed through the whole cultivation cycle (as true Ji mushroom is taken 120 days, Asparagus takes 55 days), after fruiting, can show that raw material are on producing the impact of bacterial classification, from the time, greatly shorten, affect thereby reduced bad raw material the economic loss that product yield and quality cause;
(3) the present invention is easy and simple to handle, only need can complete at laboratory stage, need to, according to normal manufacturing process of edible fungi (as bottling, sterilizing, cooling inoculation), just can not judge easy, quickly raw material quality quality.
Below in conjunction with specific embodiment, the present invention is described in further detail
Embodiment 1
By two kinds of corncobs (common commercially available), label 1 and 2 carries out pretreatment, pulverizes 5 minutes with micromill, crosses 20 mesh sieves for subsequent use.Proportionally take the corncob after sieving with electronic scale, mix with water, material water quality is than being 0.26:1,75 DEG C of water temperature controls, lixiviate 30min.With 4 ?6 layers of filtered through gauze leaching liquor, get leaching liquor after filtration for subsequent use.
By leaching liquor with distilled water mixing constant volume to 1000ml, ratio is leachate: distilled water=1.0:1, the PDA powder that takes 30g adds in mixed liquor, stir it is fully dissolved.Then the inspection medium preparing is packed in triangular flask into sterilizing, cooling, inoculation (flat board).
After inoculation hypsizygus marmoreus bacterial classification (Academy of Agricultural Sciences, Shanghai City), putting into the constant incubator of 23 DEG C cultivates, carried out observed and recorded mycelia growing way and growth rate every 2 days, when the fastest bacterial classification of growth is about to cover with plating medium in processing taking same lot inspection, as inspection terminal, about 14 days time, all processing is measured colony diameter, recorded mycelial growth potential and colonial morphology.
Note: the data in upper table are the mean value of 5 repeated tests.
According to above-mentioned result of the test, can show that No. 1, the corncob of sample presentation is superior in quality, can put into production use, No. 2 mycelial growths of corncob are slower, not vigorous, and all once bad, quality is not good, will not accept.
Embodiment 2
The rice bran of sample presentation and corn flour (being common commercially available) are carried out to pretreatment, pulverize 2 minutes with micromill, cross 40 mesh sieves for subsequent use.Proportionally take the corncob after sieving with electronic scale, mix with water, material-water ratio is 0.2:1,65 DEG C of water temperature controls, lixiviate 20min.With 4 ?6 layers of filtered through gauze leaching liquor, get leaching liquor after filtration for subsequent use.
By leaching liquor with distilled water mixing constant volume to 1000ml, ratio is leachate: distilled water=0.65:1, the PDA powder that takes 30g adds in mixed liquor, stir it is fully dissolved.Then the inspection medium preparing is packed in triangular flask into sterilizing, cooling, inoculation (flat board).
After inoculation Asparagus bacterial classification (Academy of Agricultural Sciences, Shanghai City), putting into the constant incubator of 19 DEG C cultivates, carried out observed and recorded mycelia growing way and growth rate every 2 days, when the fastest bacterial classification of growth is about to cover with plating medium in processing taking same lot inspection, as inspection terminal, about 8 days time, all processing is measured colony diameter, recorded mycelial growth potential and colonial morphology.
Note: the data in upper table are the mean value of 5 repeated tests.
According to above-mentioned result of the test, can show that the rice bran of sample presentation and the result that corn flour is verified by this method of inspection are all better than check experiment, can judge that this batch of rice bran and corn flour are superior in quality, can drop into the large production and application of Asparagus.
In addition, after each inspection of raw and process materials test, assay input computer is stayed to shelves, the check test in later stage can compare with assay and the production and application effect thereof in early stage, also can be used as the reference frame that final mass is judged.
Claims (2)
1. a method of inspection for edible mushroom raw material quality, is characterized in that comprising the steps:
Pre-treatment of raw material; The lixiviate of raw material hot water; Preparation inspection medium, sterilizing, cooling; Inoculation, cultivation, quality comprehensive are passed judgment on.
2. the method for inspection of edible mushroom raw material quality according to claim 1, is characterized in that comprising the steps:
(1) pre-treatment of raw material
Raw material are pulverized, crossed 10～40 order sieves;
(2) raw material hot water lixiviate
Raw material after pulverizing and sieving are put into water lixiviate, and material-water ratio is 0.2～0.3:1, water temperature be 60 ?85 DEG C, extraction time be 25 ?30min, get leachate for subsequent use;
(3) preparation inspection medium, sterilizing, cooling
Inspection culture medium prescription be PDA powder and culture medium solution, wherein the weight percent content of PDA powder be 2 ?4%, wherein in culture medium solution, comprise raw material leachate and distilled water, leachate: distilled water=0.6～1.0:1;
(4) inoculation, cultivation, quality comprehensive are passed judgment on
Detect when operation, add do a PDA medium contrast (medium contrast is made up of PDA powder and water, the weight percent content of PDA powder be 2 ?4%.) process, testing result is compared with contrasting, according to mycelial growth rate, mycelial growth potential, the uniformity, judge raw material quality quality.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651568A (en) * | 2004-02-03 | 2005-08-10 | 李勇 | Edible fungus liquid culture submerged fermentation technology |
| CN102498935A (en) * | 2011-10-14 | 2012-06-20 | 福建农林大学 | Method for preparing flammulina velutipes nutrient solution by utilizing remains of food processing |
| CN103168625A (en) * | 2013-04-18 | 2013-06-26 | 河南邦友农业生态循环发展有限公司 | Large-scale production method for preparing edible fungi strains |
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2014
- 2014-09-15 CN CN201410469334.3A patent/CN104186204A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651568A (en) * | 2004-02-03 | 2005-08-10 | 李勇 | Edible fungus liquid culture submerged fermentation technology |
| CN102498935A (en) * | 2011-10-14 | 2012-06-20 | 福建农林大学 | Method for preparing flammulina velutipes nutrient solution by utilizing remains of food processing |
| CN103168625A (en) * | 2013-04-18 | 2013-06-26 | 河南邦友农业生态循环发展有限公司 | Large-scale production method for preparing edible fungi strains |
Non-Patent Citations (4)
| Title |
|---|
| 姜明等: "不同比例滑菇菌糠提取液对几种食用菌菌丝生长的影响", 《黑龙江农业科学》 * |
| 李俐俐等: "平菇菌糠提取液对5种食用菌菌丝生长的影响", 《安徽农业科学》 * |
| 王轶群: "新疆特有食用菌菌糖再利用关键技术研究", 《新疆大学硕士毕业论文，中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 王轶群等: "阿魏菇菌糠提取液对四种食用菌菌丝生长的影响", 《生物技术》 * |
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Application publication date: 20141210 |