CN109055232A - A kind of phlebopus portentosus preparation method - Google Patents
A kind of phlebopus portentosus preparation method Download PDFInfo
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- CN109055232A CN109055232A CN201810873281.XA CN201810873281A CN109055232A CN 109055232 A CN109055232 A CN 109055232A CN 201810873281 A CN201810873281 A CN 201810873281A CN 109055232 A CN109055232 A CN 109055232A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention provides a kind of preparation method of Phlebopus portentosus in view of the deficiencies in the prior art.This method provides include the preparation process from parent species, shaking flask to fermentation tank culture, and propose slant medium, Shake flask medium and the unique composition of fermentation medium with preferable technical effect, reduce the fussy degree of breeding cost and culture medium preparation, the stability of Spawn incubation is improved, and mycelial growth rate is fast, yield is high, bacterium ball size is uniform, bacterium solution clear, strain activity is strong, and inoculation can cover with for cultivation base 20~25 days.
Description
Technical field
The present invention relates to field of biotechnology, especially phlebopus portentosus cultural method.
Background technique
Phlebopus portentosus is a kind of Rare edible fungus, and delicious flavour is full of nutrition, is had as a kind of edible mushroom
Vast market prospect.However, such mushroom quantity grown naturally is very limited, to meet consumer for dun net handle ox
The demand of liver bacterium needs to develop a kind of cultivation method.With in recent years for Phlebopus portentosus cultivating and growing skill
The development of art research can have been planted with bag at present and the method for bottle cultivation carries out medium scale indoor growing, greenhouse cultivation and hayashishita
Bionic cultivation also has greater advance.
However, the prior art still fails to overcome technical problem, realizes large-scale plantation.Phlebopus portentosus at present
Cultural method still uses the solid culture method based on sawdust, grain, and solid spawn cultivation cycle is long, at high cost, pollution
Detection is difficult, and bacterium germination speed is slow, and fruiting uniformity is poor, and the culture week that Phlebopus portentosus solid spawn needs 40~50 days
Phase, pollution risk are high；So it is to realize one of the key precondition cultivated on a large scale that liquid spawn, which stablizes culture, for dun net
There has also been some progress at present for the strain cultivation of handle bolete, however, phlebopus portentosus liquid bacterial culture at present
For method still based on test-type cultural method, technical solution itself is existing insufficient, such as culture medium preparation section is complicated,
Cost of material is high, high inoculum concentration, and the factors such as stability difference limit application of this method in extensive cultivation between batch, leads to it
The factorial production can be not yet extended to,
Therefore, there is an urgent need to a kind of more simple and effectives, the Phlebopus portentosus preparation that can be realized factory culture
Method.
Summary of the invention
In view of the deficiencies in the prior art, the invention proposes a kind of Phlebopus portentosus from parent species, shaking flask to
The preparation method of fermentation tank culture, this method provides the preparation process from parent species, shaking flask to fermentation tank culture, and propose tool
Formed by the slant medium, Shake flask medium and fermentation medium of preferable technical effect are unique, reduce breeding cost and
The fussy degree of culture medium preparation, improves the stability of Spawn incubation, and mycelial growth rate is fast, and yield is high, bacterium ball size
Uniform, bacterium solution clear, strain activity is strong, and inoculation can cover with for cultivation base 20~25 days.
The present invention is achieved by the following technical solutions:
A kind of phlebopus portentosus preparation method, comprising the following steps:
(1) Phlebopus portentosus parent species are prepared: taking Phlebopus portentosus strain inoculated female in obtaining on slant medium
Kind；
(2) cultivate shaking flask strain: the inclined-plane parent species for taking step (1) to prepare, related slant medium are inoculated into equipped with liquid
In the culture bottle of Shake flask medium, culture obtains shaking flask strain；
(3) the shaking flask strain that step (2) are cultivated cultivation and fermentation tank cultivar: is inoculated into the fermentation equipped with fermentation medium
In tank, cultivar is cultivated to obtain；The fermentation medium includes following component, and component ratio are as follows: every 1000ml water matches molasses
20~30g, 5~10g of sucrose, 10~20g of bean cake powder, KH2PO31~2g, MgSO41~2g, 1~3g of yeast extract, defoaming agent 2
~5g, pH value are 5.8~6.8.
Further, the fermentation medium the preparation method comprises the following steps: by the molasses, bean cake powder, yeast extract and
Defoaming agent is added to the water according to the ratio to be diluted and is beaten, and is obtained mixed serum, is added sucrose, KH2PO3、MgSO4
Stirring adds water constant volume to being completely dissolved, and adjusts pH value to 5.8~6.8, obtains fermentation medium.
Further, the fermentation medium is in phlebopus portentosus liquid bacterial culture and/or ferment tank
Purposes in culture.
Further, the condition of culture of the step (1) are as follows: under 28 DEG C~30 DEG C constant temperatures, half-light culture 25~
28 days, obtain parent species；
In the step (2), parent species related slant medium in inclined-plane prepared by step (1) is inoculated into equipped with liquid shaking bottle
Before the culture bottle of culture medium, first parent species related slant medium in inclined-plane is crushed, inoculum concentration is liquid submerged culture matrix product
The 0.5~2% of percentage, condition of culture are as follows: 28 DEG C~30 DEG C constant temperature, 130~170r/min Clothoid type concussion rate, half-light,
900~1500ppm of gas concentration lwevel；Obtain shaking flask strain within culture 6~8 days；
In the step (3), inoculum concentration is fermentation medium percent by volume 0.5~2%, condition of culture are as follows: 28~30
DEG C constant temperature, 0.02~0.05MPa of pressure, 100~150r/min of stirring rate, half-light；Obtain cultivar within culture 5~7 days.
Further, slant medium described in step (1) includes following component, and ratio between ingredient are as follows: every
1000ml water matches 20~30g of molasses, 5~10g of sucrose, KH2PO31~2g, MgSO41~2g, 16~18g of agar, yeast extract 1
~3g, pH value are 5.8~6.8.
Further, liquid submerged culture base described in step (2) includes following component, and component ratio are as follows: every
1000ml water matches 20~30g of molasses, 5~10g of sucrose, KH2PO31~2g, MgSO41~2g, 1~3g of yeast extract, pH value are
5.8~6.8.
Further, the slant medium, Shake flask medium, fermentation medium also go out before the use
Bacterium wherein sterilizes 30 minutes for 115 DEG C of slant medium, and 115 DEG C of Shake flask medium sterilize 30 minutes, fermentation medium 115~125
DEG C sterilizing 30~60 minutes.
Further, Phlebopus portentosus bacterial strain described in step (1) can separate dun net handle with tissue isolation
Bolete fructification tissue block, it is also possible to the bacterial strain mycelia block of low-temperature preservation.
Further, fermentor described in step (3) is airlift fermentor, and ferment 20~2000L of tankage size.
Further, step can be directly accessed when the fermentation tankage size is less than or equal to 300L in step (3)
(2) the shaking flask strain cultivated；When the fermentation tankage size is more than 300L, carry out expanding numerous rear access step using seed fermentation tank
(2) the shaking flask strain cultivated, the expansion are numerous are as follows: the seeding tank for selecting 10% volume of fermentor shakes what is cultivated in step (2)
Bottle strain is inoculated into seeding tank, inoculum concentration and the same step of condition of culture (3), accesses in fermentor after 5~7 days cover with, culture 5
It is mature up to strain.
A kind of phlebopus portentosus preparation method provided by the invention has following technical effect that
(1) breeding cost substantially reduces, and culture medium preparation is simple and fast, improves the stability of Spawn incubation；
(2) mycelial growth rate is fast, and yield is high, and bacterium ball size is uniform, and bacterium solution clear, strain activity is strong, and inoculation is planted
Training can cover with for base 20~25 days.
Specific embodiment
In this part, further explanation and description of the technical solution of the present invention are carried out.It is pointed out that this reality
It applies in example only as explaining and illustrating, is not construed as limiting the invention, embodiments of the present invention are also not limited to following one kind
Or it is several.
Embodiment 1
A kind of phlebopus portentosus preparation method, comprising the following steps:
(1) Phlebopus portentosus parent species are prepared: by slant medium at 115 DEG C sterilization treatment 30 minutes, take group
The Phlebopus portentosus bacterial strain tissue block for knitting partition method preparation is inoculated on slant medium, under 28 DEG C of constant temperatures, secretly
Optical culture 28 days.
The inclined-plane culture based component and content are as follows: water 1000ml, molasses 20g, sucrose 5g, KH2PO31g、MgSO41g、
Agar 16g, yeast extract 1g, pH value 5.8.
(2) culture of shaking flask strain: by fluid nutrient medium at 115 DEG C sterilization treatment 30 minutes, take inclined-plane parent species, it is related
Slant medium crushes, and according to 2% inoculum concentration of fluid nutrient medium percent by volume, is inoculated into equipped with 400ml Liquid Culture
In the 500ml culture bottle of base, shaking flask strain is obtained within culture 8 days；Condition of culture are as follows: 28 DEG C of constant temperature, 170r/min Clothoid type concussion speed
Rate, half-light, gas concentration lwevel 1500ppm.
The liquid submerged culture based component and content are as follows: water 1000ml, molasses 20g, sucrose 5g, KH2PO31g、
MgSO41g, yeast extract 1g, pH value 5.8.
(3) fermentor cultivar culture: by fermentation medium at 115 DEG C sterilization treatment 30 minutes, shaking flask strain is pressed
The inoculum concentration of fermentation medium percent by volume 2% is inoculated into fermentor, and the capacity of fermentor is 20L, and culture must cultivate for 5 days
Kind.Condition of culture: 28 DEG C of constant temperature, pressure 0.02MPa, stirring rate 100r/min, half-light.
Fermentation medium includes following component: molasses 30g, sucrose 5g, bean cake powder 20g, KH2PO3 1g, MgSO4 1g, ferment
Female medicinal extract 3g, defoaming agent 5g, water 1000ml.The preparation method comprises the following steps: the molasses, bean cake powder, yeast extract and defoaming agent are pressed
According to the ratio be added to the water dilute and be beaten, obtain mixed serum, add sucrose, KH2PO3, MgSO4 stir to
It is completely dissolved, adds water constant volume, adjust pH value to 5.8, obtain fermentation medium.
Embodiment 2
A kind of phlebopus portentosus preparation method, comprising the following steps:
(1) preparation of Phlebopus portentosus parent species: by slant medium at 115 DEG C sterilization treatment 30 minutes, take low
The Phlebopus portentosus bacterial strain inoculated by hypha block of warm preservation is on slant medium, under 29 DEG C of constant temperatures, half-light culture
26 days.
The inclined-plane culture based component and content are as follows: water 1000ml water, molasses 30g, sucrose 10g, KH2PO3 2g,
MgSO4 2g, agar 18g, yeast extract 3g, pH value 6.8.
(2) culture of shaking flask strain: by slant medium at 115 DEG C sterilization treatment 30 minutes, take inclined-plane parent species, it is related
Culture medium crushes, and according to 1% inoculum concentration of fluid nutrient medium percent by volume, is inoculated into equipped with 400ml fluid nutrient medium
In 500ml culture bottle, shaking flask strain is obtained within culture 7 days；Condition of culture are as follows: 29 DEG C of constant temperature, 150r/min Clothoid type shake rate, secretly
Light, gas concentration lwevel 1000ppm.
The liquid submerged culture based component and ratio are as follows: water 1000ml, molasses 30g, sucrose 10g, KH2PO3 2g,
MgSO4 2g, yeast extract 3g, pH value 6.8.
(3) fermentor cultivar culture: by fermentation medium at 125 DEG C sterilization treatment 60 minutes, shaking flask strain is pressed
The inoculum concentration of fermentation medium percent by volume 1% is inoculated into fermentor, and fermentation tankage size is 200L, and culture must cultivate for 6 days
Kind.Condition of culture: 29 DEG C of constant temperature, pressure 0.03MPa, stirring rate 120r/min, half-light.
Fermentation medium include following component: molasses 20g, sucrose 10g, bean cake powder 10g, KH2PO3 2g, MgSO4 2g,
Yeast extract 1g, defoaming agent 2g, water 1000ml.The preparation method comprises the following steps: by the molasses, bean cake powder, yeast extract and defoaming agent
It is added to the water according to the ratio and dilutes and be beaten, obtain mixed serum, add sucrose, KH2PO3, MgSO4 stirring
To being completely dissolved, add water constant volume, adjusts pH value to 6.8, obtain fermentation medium.
Embodiment 3
A kind of phlebopus portentosus preparation method, comprising the following steps:
(1) preparation of Phlebopus portentosus parent species: by slant medium at 115 DEG C sterilization treatment 30 minutes, take low
The Phlebopus portentosus bacterial strain inoculated by hypha block of warm preservation is on slant medium, under 30 DEG C of constant temperatures, half-light culture
25 days.
The ingredient and ratio of the slant medium are as follows: water 1000ml, molasses 28g, sucrose 8g, KH2PO31.5g、
MgSO41.5g, agar 17g, yeast extract 2g, pH value 6.
(2) culture of shaking flask strain: by slant medium at 115 DEG C sterilization treatment 30 minutes, take inclined-plane parent species, it is related
Culture medium crushes, and according to 0.5% inoculum concentration of fluid nutrient medium percent by volume, is inoculated into equipped with 250ml fluid nutrient medium
500ml culture bottle in, culture 6 days shaking flask strain；Condition of culture are as follows: 30 DEG C of constant temperature, 130r/min Clothoid type shake rate,
Half-light, gas concentration lwevel 900ppm.
The ingredient and ratio of the liquid submerged culture base are as follows: water 1000ml, molasses 25g, sucrose 7g, KH2PO3
1.5g、MgSO41.5g, yeast extract 1.5g, pH value 6.2.
(3) fermentor cultivar culture: by fermentation medium at 120 DEG C sterilization treatment 40 minutes, shaking flask strain is pressed
The inoculum concentration of fermentation medium percent by volume 0.5% is inoculated into fermentor, and fermentation tankage size is 250L, and culture must plant for 6 days
It cultivates.Condition of culture: 30 DEG C of constant temperature, pressure 0.05MPa, stirring rate 150r/min, half-light.
Fermentation medium includes following component: molasses 28g, sucrose 7g, bean cake powder 11g, KH2PO3 1.9g, MgSO4
1.6g, yeast extract 2g, defoaming agent 4g, water 1000ml.The preparation method comprises the following steps: by the molasses, bean cake powder, yeast extract and disappearing
Infusion is added to the water according to the ratio to be diluted and is beaten, and is obtained mixed serum, is added sucrose, KH2PO3, MgSO4
Stirring adds water constant volume to being completely dissolved, and adjusts pH value to 6.2, obtains fermentation medium.
Embodiment 4
A kind of phlebopus portentosus preparation method, comprising the following steps:
(1) preparation of Phlebopus portentosus parent species: by slant medium at 115 DEG C sterilization treatment 30 minutes, take
The Phlebopus portentosus bacterial strain tissue block of tissue isolation preparation is inoculated on slant medium, under 30 DEG C of constant temperatures,
Half-light culture 25 days.
The inclined-plane culture based component and ratio are as follows: water 1000ml, molasses 30g, sucrose 10g, KH2PO3 1.5g,
MgSO4 1.5g, agar 18g, yeast extract 1g, pH value 5.8.
(2) culture of shaking flask strain: by slant medium at 115 DEG C sterilization treatment 30 minutes, take inclined-plane parent species, it is related
Culture medium crushes, and according to 0.5% inoculum concentration of fluid nutrient medium percent by volume, is inoculated into equipped with 250ml fluid nutrient medium
500ml culture bottle in, culture 6 days shaking flask strain；Condition of culture are as follows: 30 DEG C of constant temperature, 130r/min Clothoid type shake rate,
Half-light, gas concentration lwevel 900ppm.
The liquid submerged culture based component and ratio are as follows: water 1000ml water, molasses 20g, sucrose 10g, KH2PO3
2g, MgSO4 2g, yeast extract 1g, pH value 6.8.
(3) fermentor cultivar culture: by fermentation medium at 115 DEG C sterilization treatment 30 minutes, shaking flask strain is pressed
The inoculum concentration of fermentation medium percent by volume 0.5% is inoculated into fermentor, and fermentation tankage size is 300L, and culture must plant for 7 days
It cultivates.Condition of culture: 30 DEG C of constant temperature, pressure 0.05MPa, stirring rate 150r/min, half-light.
Fermentation medium include following component: molasses 20g, sucrose 10g, bean cake powder 10g, KH2PO3 2g, MgSO4 2g,
Yeast extract 1g, defoaming agent 2g, water 1000ml.The preparation method comprises the following steps: by the molasses, bean cake powder, yeast extract and defoaming agent
It is added to the water according to the ratio and dilutes and be beaten, obtain mixed serum, add sucrose, KH2PO3, MgSO4 stirring
To being completely dissolved, add water constant volume, adjusts pH value to 6.8, obtain fermentation medium.
Embodiment 5
The present embodiment compared with Example 4, the difference is that:
In step (3) when used fermentation tankage size is 2000L, selecting capacity is that the seeding tank of 200L is expanded
Numerous, inoculum concentration and condition of culture access in fermentor after 5 days cover with embodiment 4, cultivate 5 days mature strains to obtain the final product.
Embodiment 6
The present embodiment compared with Example 4, the difference is that:
In step (3) when used fermentation tankage size is 1000L, selecting capacity is that the seeding tank of 100L is expanded
Numerous, inoculum concentration and condition of culture access in fermentor after 7 days cover with embodiment 4, cultivate 5 days mature strains to obtain the final product.
Embodiment 7
The present embodiment compared with Example 4, the difference is that:
In step (3) when used fermentation tankage size be 500L when, select capacity be 50L seeding tank expand it is numerous,
Inoculum concentration and condition of culture access in fermentor after 6 days cover with embodiment 4, cultivate 5 days mature strains to obtain the final product.
Embodiment 8
A kind of phlebopus portentosus preparation method, it is characterised in that: the following steps are included:
(1) Phlebopus portentosus parent species are prepared: taking Phlebopus portentosus strain inoculated female in obtaining on slant medium
Kind；Any culture medium that can be used for preparing bolete parent species in the prior art can be used in slant medium.
(2) cultivate shaking flask strain: the inclined-plane parent species for taking step (1) to prepare, related slant medium are inoculated into equipped with liquid
In the culture bottle of Shake flask medium, culture obtains shaking flask strain；Liquid submerged culture base can be used any available in the prior art
In the culture medium of preparation bolete shaking flask strain.
(3) the shaking flask strain that step (2) are cultivated cultivation and fermentation tank cultivar: is inoculated into the fermentation equipped with fermentation medium
In tank, cultivar is cultivated to obtain；The fermentation medium includes following component, and component ratio are as follows: every 1000ml water matches molasses
20g, sucrose 5g, bean cake powder 10g, KH2PO31g、MgSO41g, yeast extract 1g, defoaming agent 2g, pH value 5.8.
Embodiment 9
Compared with Example 8, the present embodiment the difference is that: fermentation medium components and ratio in step (3)
Are as follows: every 1000ml water matches molasses 30g, sucrose 10g, bean cake powder 20g, KH2PO32g、MgSO42g, yeast extract 3g, defoaming agent 5g,
PH value is 6.8.
Embodiment 10
Compared with Example 8, the present embodiment the difference is that: fermentation medium components and ratio in step (3)
Are as follows: every 1000ml water matches molasses 22g, sucrose 7g, bean cake powder 17g, KH2PO31.5g、MgSO41.2g, yeast extract 1.4g, defoaming
Agent 4g, pH value 6.1.
Claims (10)
1. a kind of phlebopus portentosus preparation method, it is characterised in that: the following steps are included:
(1) Phlebopus portentosus parent species are prepared: taking Phlebopus portentosus strain inoculated on slant medium, obtains parent species；
(2) cultivate shaking flask strain: the inclined-plane parent species for taking step (1) to prepare, related slant medium are inoculated into equipped with liquid shaking bottle
In the culture bottle of culture medium, culture obtains shaking flask strain；
(3) the shaking flask strain that step (2) are cultivated cultivation and fermentation tank cultivar: is inoculated into the fermentor equipped with fermentation medium
In, cultivate to obtain cultivar；The fermentation medium includes following component, and component ratio are as follows: every 1000ml water matches molasses 20
~30g, 5~10g of sucrose, 10~20g of bean cake powder, KH2PO31~2g, MgSO41~2g, 1~3g of yeast extract, defoaming agent 2
~5g, pH value are 5.8~6.8.
2. a kind of phlebopus portentosus preparation method described in claim 1, it is characterised in that: the fermented and cultured
Base the preparation method comprises the following steps: the molasses, bean cake powder, yeast extract and defoaming agent are added to the water according to the ratio dilute
It releases and is beaten, obtain mixed serum, add sucrose, KH2PO3、MgSO4Stirring adds water constant volume to being completely dissolved, and adjusts pH value
To 5.8~6.8, fermentation medium is obtained.
3. a kind of phlebopus portentosus preparation method as claimed in claim 1 or 2, it is characterised in that: the hair
Purposes of the ferment culture medium in phlebopus portentosus liquid bacterial culture and/or ferment tank culture.
4. a kind of phlebopus portentosus preparation method as claimed in claim 1 or 2, it is characterised in that:
The condition of culture of the step (1) are as follows: under 28 DEG C~30 DEG C constant temperatures, half-light culture 25~28 days, obtain parent species；
In the step (2), parent species related slant medium in inclined-plane prepared by step (1) is inoculated into equipped with liquid submerged culture
Before the culture bottle of base, first parent species related slant medium in inclined-plane is crushed, inoculum concentration is liquid submerged culture base volume basis
The 0.5~2% of ratio, condition of culture are as follows: 28 DEG C~30 DEG C constant temperature, 130~170r/min Clothoid type shake rate, half-light, dioxy
Change 900~1500ppm of concentration of carbon；Obtain shaking flask strain within culture 6~8 days；
In the step (3), inoculum concentration is fermentation medium percent by volume 0.5~2%, condition of culture are as follows: 28~30 DEG C of perseverances
Temperature, 0.02~0.05MPa of pressure, 100~150r/min of stirring rate, half-light；Obtain cultivar within culture 5~7 days.
5. a kind of phlebopus portentosus preparation method as claimed in claim 4, it is characterised in that: step (1) is described
Slant medium include following component, and ratio between ingredient are as follows: every 1000ml water with 20~30g of molasses, 5~10g of sucrose,
KH2PO31~2g, MgSO41~2g, 16~18g of agar, 1~3g of yeast extract, pH value are 5.8~6.8.
6. a kind of phlebopus portentosus preparation method as described in claim 4 or 5, it is characterised in that: in step (2)
The liquid submerged culture base includes following component, and component ratio are as follows: every 1000ml water with 20~30g of molasses, sucrose 5~
10g、KH2PO31~2g, MgSO41~2g, 1~3g of yeast extract, pH value are 5.8~6.8.
7. a kind of phlebopus portentosus preparation method as claimed in claim 4, it is characterised in that: the inclined-plane training
It supports base, Shake flask medium, fermentation medium also to sterilize before the use, wherein 115 DEG C of slant medium sterilizings 30
Minute, 115 DEG C of Shake flask medium sterilize 30 minutes, and 115~125 DEG C of fermentation medium sterilize 30~60 minutes.
8. a kind of phlebopus portentosus preparation method as claimed in claim 4, it is characterised in that: institute in step (1)
The Phlebopus portentosus bacterial strain stated can separate Phlebopus portentosus fructification tissue block with tissue isolation, it is also possible to low temperature
The bacterial strain mycelia block of preservation.
9. a kind of phlebopus portentosus preparation method as claimed in claim 4, it is characterised in that: institute in step (3)
The fermentor stated is airlift fermentor, and ferment 20~2000L of tankage size.
10. a kind of phlebopus portentosus preparation method as claimed in claim 8, it is characterised in that: in step (3) when
When the fermentation tankage size is less than or equal to 300L, the shaking flask strain of step (2) culture can be directly accessed；The fermentor
When capacity is more than 300L, expand using seed fermentation tank the shaking flask strain of numerous rear access step (2) culture, the expansion is numerous
Are as follows: the shaking flask strain cultivated in step (2) is inoculated into seeding tank by the seeding tank for selecting 10% volume of fermentor, inoculum concentration and
The same step of condition of culture (3) accesses in fermentor after 5~7 days cover with, and cultivates 5 days mature strains to obtain the final product.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651568A (en) * | 2004-02-03 | 2005-08-10 | 李勇 | Edible fungus liquid culture submerged fermentation technology |
| CN101381684A (en) * | 2008-10-22 | 2009-03-11 | 云南省热带作物科学研究所 | Culture method of phlebopus portentosus liquid bacterial |
| CN101491195A (en) * | 2009-03-05 | 2009-07-29 | 云南省热带作物科学研究所 | Phlebopus portentosus cultivation method |
| JP2009240276A (en) * | 2008-03-31 | 2009-10-22 | Naris Cosmetics Co Ltd | Liquid culture medium for boletaceae suillus basidiomycete |
| JP2009240275A (en) * | 2008-03-31 | 2009-10-22 | Naris Cosmetics Co Ltd | Liquid culture medium for boletaceae suillus basidiomycete |
| CN103430773A (en) * | 2013-08-28 | 2013-12-11 | 云南省热带作物科学研究所 | Method for preserving deep brown boletus reticulatus strains |
| CN105567576A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Liquid strain breeding method and field bionic cultivation method for bolete |
| CN106929432A (en) * | 2017-03-09 | 2017-07-07 | 云南省热带作物科学研究所 | A kind of phlebopus portentosus liquid bacterial submerged fermentation new method |
| CN107162753A (en) * | 2017-06-14 | 2017-09-15 | 广东东阳光药业有限公司 | The culture medium for cultivating and method of Phlebopus portentosus |
-
2018
- 2018-08-02 CN CN201810873281.XA patent/CN109055232A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651568A (en) * | 2004-02-03 | 2005-08-10 | 李勇 | Edible fungus liquid culture submerged fermentation technology |
| JP2009240276A (en) * | 2008-03-31 | 2009-10-22 | Naris Cosmetics Co Ltd | Liquid culture medium for boletaceae suillus basidiomycete |
| JP2009240275A (en) * | 2008-03-31 | 2009-10-22 | Naris Cosmetics Co Ltd | Liquid culture medium for boletaceae suillus basidiomycete |
| CN101381684A (en) * | 2008-10-22 | 2009-03-11 | 云南省热带作物科学研究所 | Culture method of phlebopus portentosus liquid bacterial |
| CN101491195A (en) * | 2009-03-05 | 2009-07-29 | 云南省热带作物科学研究所 | Phlebopus portentosus cultivation method |
| CN103430773A (en) * | 2013-08-28 | 2013-12-11 | 云南省热带作物科学研究所 | Method for preserving deep brown boletus reticulatus strains |
| CN105567576A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Liquid strain breeding method and field bionic cultivation method for bolete |
| CN106929432A (en) * | 2017-03-09 | 2017-07-07 | 云南省热带作物科学研究所 | A kind of phlebopus portentosus liquid bacterial submerged fermentation new method |
| CN107162753A (en) * | 2017-06-14 | 2017-09-15 | 广东东阳光药业有限公司 | The culture medium for cultivating and method of Phlebopus portentosus |
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