CN107493970A - A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation - Google Patents

A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation Download PDF

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Publication number
CN107493970A
CN107493970A CN201710839435.9A CN201710839435A CN107493970A CN 107493970 A CN107493970 A CN 107493970A CN 201710839435 A CN201710839435 A CN 201710839435A CN 107493970 A CN107493970 A CN 107493970A
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bacterium
lichee
glossy ganoderma
cultivation
slurry
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曹丽萍
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Foshan Sanshui Charles Agricultural Technology Research Institute (general Partner)
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms

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  • General Engineering & Computer Science (AREA)
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  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation, the described method comprises the following steps：(1) strain selects；(2) cultural hypha：Lichee bacteria cultivation kind obtained by step (1) is inoculated on glossy ganoderma mycelium fermentation bacterium slurry and cultivates 30 60 days to obtain lichee bacterium mycelium pellet；(3) lichee bacteria cultivation：It is artificial to build greenhouse, whole furrow, glossy ganoderma mycelium fermentation bacterium slurry is spread, be placed on glossy ganoderma mycelium fermentation bacterium slurry and cultivate after lichee bacterium mycelium pellet obtained by step (2) is disperseed, periodically sprayed water, divulge information, imposing illumination until lichee bacterium is ripe.Beneficial effects of the present invention are：Using glossy ganoderma mycelium fermentation slurry as the matrix of lichee bacteria cultivation, the artificial cultivation of lichee bacterium is realized, overcomes dependence of the lichee bacterium to termitary；The content of calcium in lichee bacterium can be improved；It is simple to plant technique, easily promotes, there is major economic value.

Description

A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation
Technical field
The present invention relates to wild mushroom artificial cultivation technique field, and in particular to one kind utilizes glossy ganoderma mycelium fermentation slurry cultivation The method of lichee bacterium.
Background technology
Lichee bacterium (Litchi chinensis Bacterium) is grown on the lichee woods humidity termitary of In Guangdong Province A kind of wild mushroom, be known as the title of " south of the Five Ridges bacterium king ", be the food materials for making Cantonese take pride in the most, be also in " chicken fir " mushroom generally acknowledge Most delicious mushroom, growth cycle only have 20 my god, annual lunar calendar early May at the beginning, terminates, often when the Summer Solstice, lichee maturation Year is all short-lived, and growth need to rely on the presence of termitary, once termite migrates, even if other conditions are constant, same The place coming year will not also grow lichee bacterium, and lichee bacterium and termite are how symbiosis is still unaware of, therefore, current rare and litchi The related report of branch bacterium artificial cultivation technique.
Application number 201611128223.1 discloses a kind of side that lichee bacterium is cultivated using Uricularia polytricha cultivation matrix leaching liquor Method, comprise the following steps：(1) termite is bred, Uricularia polytricha cultivation is during which injected in soil by 0.5~1.5h/d frequency pipe Matrix leaching liquor is trained, the Uricularia polytricha cultivation matrix includes mango wood chip；(2) lichee bacterium is cultivated.The invention is by manually taking Flowing injection Uricularia polytricha cultivation matrix leaching liquor, utilizes effective chemicals that termite can be lured in leaching liquor in the termitary built Matter, naturally prevent termite from migrating, it is ensured that with the interaction that exchanges of termite during lichee bacteria growing, while to be carried for the growth of lichee bacterium For nutriment, the wild taste of lichee bacterium can be effectively retained, realizes its artificial culture.Although the invention realizes the people of lichee bacterium Work is cultivated, but still depends on the presence of termitary, and long-term use of Uricularia polytricha cultivation matrix leaching liquor lures termite, attractant effect Easily increasingly reduce, the final cultivation for influenceing lichee bacterium.
The content of the invention
To solve the above-mentioned problems in the prior art, it is an object of the invention to provide one kind to utilize ganoderma lucidum mycelium The method that fermentation slurry cultivates lichee bacterium, to overcome dependence of the lichee bacterium to termitary, only starched by glossy ganoderma mycelium fermentation real The artificial cultivation of existing lichee bacterium.
For achieving the above object, the technical solution adopted by the present invention is as follows：
A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation, the described method comprises the following steps：
(1) strain selects：Wild Lychee bacterium is chosen as introduces a collection, parent species is separated, lichee bacteria cultivation kind is obtained through Secondary Culture；
(2) cultural hypha：Lichee bacteria cultivation kind obtained by step (1) is inoculated on glossy ganoderma mycelium fermentation bacterium slurry and cultivated Obtain within 30-60 days lichee bacterium mycelium pellet；
(3) lichee bacteria cultivation：It is artificial to build greenhouse, whole furrow, glossy ganoderma mycelium fermentation bacterium slurry is spread, by obtained by step (2) It is placed on glossy ganoderma mycelium fermentation bacterium slurry and cultivates after lichee bacterium mycelium pellet is scattered, periodically sprays water, divulges information, imposing illumination until lichee Bacterium is ripe.
Further, the glossy ganoderma mycelium fermentation slurry is starched for red sesame mycelium fermentation bacterium slurry, G.japonicum mycelium zymophyte Or the camphorata mycelium fermented bacterium slurry of blood.
Further, the water periodically sprayed during methods described step (3) lichee bacteria cultivation is glossy ganoderma mycelium fermentation liquid.
Further, the preparation method of the glossy ganoderma mycelium fermentation slurry is：Activated ganoderma lucidum strain；It is 0.1- to take size 0.4cm2Activation fungus block in zymotic fluid quiescent culture 18-22h, be then placed in compound constant temperature oscillator, with 100-120 Secondary/min shaken cultivation 5-7d, obtain first class inoculum；Then first class inoculum access seed liquor vibration is trained with 7-9.5% inoculum concentration Foster 46-56h obtains seed tank culture liquid；Seed tank culture liquid is finally accessed into zymotic fluid, shaken cultivation 60-68h, filtering, supernatant Liquid is glossy ganoderma mycelium fermentation liquid, and filter residue is starched for glossy ganoderma mycelium fermentation.
Further, the culture medium of activated ganoderma lucidum strain includes the raw material of following weight part ratio in the preparation method：Portugal Grape sugar 16-19g, peptone 16-19g, CaC12 0.6-0.9g、MgSO4 0.6-0.9g、KH2PO4 0.6-0.9g、(NH4)2HPO4 0.1-0.4g、KNO30.6-0.9g, brewer's wort 90-110mL, VBl86-92 μ g, agar 16-19g, add distilled water extremely 1000mL。
Further, the zymotic fluid in the preparation method contains 30-50% black soya bean slurry.
Ganoderma lucidum (Ganoderma lucidum) is that China is used as medicine up to the famous medicinal fungi of two thousand yearses, belongs to basidiomycetes Guiding principle, Aphyllophorales, Ganodermataceae, Ganoderma are effective rich in tens kinds of polysaccharide, triterpenes, ucleosides, alkaloids, mannitol etc. Composition.
Compared with prior art, beneficial effects of the present invention are：Lichee bacteria cultivation is used as using glossy ganoderma mycelium fermentation slurry Matrix, the artificial cultivation of lichee bacterium is realized, overcome dependence of the lichee bacterium to termitary；The content of calcium in lichee bacterium can be improved；Separately Outside, periodically spray glossy ganoderma mycelium fermentation liquid can further improve the content of calcium in lichee bacterium during lichee bacteria cultivation；Plant work Skill is simple, easily promotes, has major economic value.
Embodiment
To make those skilled in the art be better understood when technical scheme, with reference to embodiment pair Technical solution of the present invention is described in detail.
Experimental method used in following embodiments is conventional method unless otherwise specified.Institute in following embodiments Material, reagent etc., unless otherwise specified, commercially obtain.
Embodiment 1
A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation, the described method comprises the following steps：
(1) strain selects：Wild Lychee bacterium is chosen as introduces a collection, parent species is separated, lichee bacteria cultivation kind is obtained through Secondary Culture；
(2) cultural hypha：Lichee bacteria cultivation kind obtained by step (1) is inoculated on glossy ganoderma mycelium fermentation bacterium slurry and cultivates 30 It obtains lichee bacterium mycelium pellet；
(3) lichee bacteria cultivation：It is artificial to build greenhouse, whole furrow, glossy ganoderma mycelium fermentation bacterium slurry is spread, by obtained by step (2) It is placed on glossy ganoderma mycelium fermentation bacterium slurry and cultivates after lichee bacterium mycelium pellet is scattered, periodically sprays water, divulges information, imposing illumination until lichee Bacterium is ripe.
Further, the glossy ganoderma mycelium fermentation slurry can be red sesame mycelium fermentation bacterium slurry, G.japonicum mycelium fermentation Bacterium is starched or the camphorata mycelium fermented bacterium slurry of blood.
Embodiment 2
A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation, the described method comprises the following steps：
(1) strain selects：Wild Lychee bacterium is chosen as introduces a collection, parent species is separated, lichee bacteria cultivation kind is obtained through Secondary Culture；
(2) cultural hypha：Lichee bacteria cultivation kind obtained by step (1) is inoculated on glossy ganoderma mycelium fermentation bacterium slurry and cultivates 45 It obtains lichee bacterium mycelium pellet；
(3) lichee bacteria cultivation：It is artificial to build greenhouse, whole furrow, glossy ganoderma mycelium fermentation bacterium slurry is spread, by obtained by step (2) It is placed on glossy ganoderma mycelium fermentation bacterium slurry and cultivates after lichee bacterium mycelium pellet is scattered, periodically sprays water, divulges information, imposing illumination until lichee Bacterium is ripe.
Further, the water periodically sprayed during methods described step (3) lichee bacteria cultivation can be glossy ganoderma mycelium fermentation Liquid.
Embodiment 3
A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation, the described method comprises the following steps：
(1) strain selects：Wild Lychee bacterium is chosen as introduces a collection, parent species is separated, lichee bacteria cultivation kind is obtained through Secondary Culture；
(2) cultural hypha：Lichee bacteria cultivation kind obtained by step (1) is inoculated on glossy ganoderma mycelium fermentation bacterium slurry and cultivates 60 It obtains lichee bacterium mycelium pellet；
(3) lichee bacteria cultivation：It is artificial to build greenhouse, whole furrow, glossy ganoderma mycelium fermentation bacterium slurry is spread, by obtained by step (2) It is placed on glossy ganoderma mycelium fermentation bacterium slurry and cultivates after lichee bacterium mycelium pellet is scattered, periodically sprays water, divulges information, imposing illumination until lichee Bacterium is ripe.
Further, the preparation method of the glossy ganoderma mycelium fermentation slurry can be：Activated ganoderma lucidum strain；The size is taken to be 0.1-0.4cm2Activation fungus block in zymotic fluid quiescent culture 18-22h, be then placed in compound constant temperature oscillator, with 100- 120 times/min shaken cultivation 5-7d, obtain first class inoculum；Then first class inoculum access seed liquor is shaken with 7-9.5% inoculum concentration Swing culture 46-56h and obtain seed tank culture liquid；Seed tank culture liquid is finally accessed into zymotic fluid, shaken cultivation 60-68h, filtered, Supernatant is glossy ganoderma mycelium fermentation liquid, and filter residue is starched for glossy ganoderma mycelium fermentation.
Further, the culture medium of activated ganoderma lucidum strain can include the original of following weight part ratio in the preparation method Material：Glucose 16-19g, peptone 16-19g, CaC12 0.6-0.9g、MgSO4 0.6-0.9g、KH2PO4 0.6-0.9g、 (NH4)2HPO4 0.1-0.4g、KNO30.6-0.9g, brewer's wort 90-110mL, VBl86-92 μ g, agar 16-19g, add distillation Water is to 1000mL.
Further, the black soya bean slurry that the zymotic fluid in the preparation method can be containing 30-50%.
Embodiment 4
A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation, the described method comprises the following steps：
(1) strain selects：Wild Lychee bacterium is chosen as introduces a collection, parent species is separated, lichee bacteria cultivation kind is obtained through Secondary Culture；
(2) cultural hypha：Lichee bacteria cultivation kind obtained by step (1) is inoculated on glossy ganoderma mycelium fermentation bacterium slurry and cultivates 52 It obtains lichee bacterium mycelium pellet；
(3) lichee bacteria cultivation：It is artificial to build greenhouse, whole furrow, glossy ganoderma mycelium fermentation bacterium slurry is spread, by obtained by step (2) It is placed on glossy ganoderma mycelium fermentation bacterium slurry and cultivates after lichee bacterium mycelium pellet is scattered, periodically sprays water, divulges information, imposing illumination until lichee Bacterium is ripe.
Cultivation gained lichee bacterium and polyoses content in wild collection lichee bacterium in embodiment 1-4 are determined using conventional method, It the results are shown in Table 1.
Cultivation gained lichee bacterium and calcium content in wild collection lichee bacterium in the embodiment 1-4 of table 1
Lichee bacterium source Calcium content (mg/100g)
Embodiment 1 26.14
Embodiment 2 26.15
Embodiment 3 26.16
Embodiment 4 23.65
Wild collection 22.89
As known from Table 1, calcium content is significantly higher than calcium in wild collection lichee bacterium and contained in embodiment 1-4 cultivations gained lichee bacterium Amount, illustrate the content that calcium in lichee bacterium can be improved using the matrix of glossy ganoderma mycelium fermentation slurry as lichee bacteria cultivation；Implement in addition Calcium content is better than embodiment 4 in example 1-3 cultivation gained lichee bacterium, illustrates periodically to spray ganoderma lucidum mycelium hair during lichee bacteria cultivation Zymotic fluid can further improve the content of calcium in lichee bacterium.
Obviously, above-described embodiment is only the section Example of the present invention, rather than whole embodiments.Based on opening for the present invention Show, those of ordinary skill in the art's all other embodiment resulting on the premise of creative work is not made, all Belong to the scope that present aspect is protected.

Claims (6)

  1. A kind of 1. method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation, it is characterised in that methods described includes following step Suddenly：
    (1) strain selects：Wild Lychee bacterium is chosen as introduces a collection, parent species is separated, lichee bacteria cultivation kind is obtained through Secondary Culture；
    (2) cultural hypha：Lichee bacteria cultivation kind obtained by step (1) is inoculated on glossy ganoderma mycelium fermentation bacterium slurry and cultivates 30-60 It obtains lichee bacterium mycelium pellet；
    (3) lichee bacteria cultivation：It is artificial to build greenhouse, whole furrow, glossy ganoderma mycelium fermentation bacterium slurry is spread, by lichee obtained by step (2) Be placed on glossy ganoderma mycelium fermentation bacterium slurry and cultivate after bacterium mycelium pellet is scattered, periodically spray water, divulge information, imposing illumination until lichee bacterium into It is ripe.
  2. 2. method according to claim 1, it is characterised in that the glossy ganoderma mycelium fermentation slurry is red sesame mycelium fermentation bacterium Slurry, G.japonicum mycelium zymophyte slurry or the camphorata mycelium fermented bacterium slurry of blood.
  3. 3. method according to claim 1, it is characterised in that periodically sprayed during methods described step (3) lichee bacteria cultivation Water is glossy ganoderma mycelium fermentation liquid.
  4. 4. according to any one of claim 1-3 methods described, it is characterised in that the preparation method of the glossy ganoderma mycelium fermentation slurry For：Activated ganoderma lucidum strain；It is 0.1-0.4cm to take size2Activation fungus block in zymotic fluid quiescent culture 18-22h, be then placed in In compound constant temperature oscillator, with 100-120 times/min shaken cultivation 5-7d, first class inoculum is obtained；Then with 7-9.5% inoculum concentration First class inoculum access seed liquor shaken cultivation 46-56h is obtained into seed tank culture liquid；Finally seed tank culture liquid is accessed and fermented Liquid, shaken cultivation 60-68h, filtering, supernatant are glossy ganoderma mycelium fermentation liquid, and filter residue is starched for glossy ganoderma mycelium fermentation.
  5. 5. method according to claim 4, it is characterised in that the culture medium of activated ganoderma lucidum strain includes in the preparation method The raw material of following weight part ratio：Glucose 16-19g, peptone 16-19g, CaC120.6-0.9g、MgSO40.6-0.9g、 KH2PO40.6-0.9g、(NH4)2HPO40.1-0.4g、KNO30.6-0.9g, brewer's wort 90-110mL, VBl86-92 μ g, agar 16-19g, add distilled water to 1000mL.
  6. 6. method according to claim 4, it is characterised in that zymotic fluid in the preparation method contain 30-50%'s Black soya bean is starched.
CN201710839435.9A 2017-09-15 2017-09-15 A kind of method that cultivation lichee bacterium is starched using glossy ganoderma mycelium fermentation Withdrawn CN107493970A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109892169A (en) * 2019-03-28 2019-06-18 上海市农业科学院 A kind of germination method of purple sesame basidiospore germination culture medium and purple sesame basidiospore

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CN1528117A (en) * 2003-10-21 2004-09-15 中国科学院植物研究所 Method for producing hedgehog fungus and special culture medium thereof
CN1651568A (en) * 2004-02-03 2005-08-10 李勇 Edible fungus liquid culture submerged fermentation technology
CN101560118A (en) * 2009-02-26 2009-10-21 浙江大学 Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method
CN101971762A (en) * 2010-02-09 2011-02-16 中国农业科学院农业资源与农业区划研究所 Liquid submerged fermentation culture method for hericium erinaceus
CN102084782A (en) * 2010-11-30 2011-06-08 忻州市汇恒农林牧综合开发有限公司 Natural planting method of mushroom
CN102396346A (en) * 2010-02-09 2012-04-04 中国农业科学院农业资源与农业区划研究所 Hericium erinaceus
CN102690138A (en) * 2012-06-21 2012-09-26 吴利明 Universal lucid ganoderma mycelia fermentation medium combination
CN105474995A (en) * 2015-12-21 2016-04-13 镇江盛弘景观植物有限公司 Cultivation and domestication method of wild collybia albuminosa

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1528117A (en) * 2003-10-21 2004-09-15 中国科学院植物研究所 Method for producing hedgehog fungus and special culture medium thereof
CN1651568A (en) * 2004-02-03 2005-08-10 李勇 Edible fungus liquid culture submerged fermentation technology
CN101560118A (en) * 2009-02-26 2009-10-21 浙江大学 Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method
CN101971762A (en) * 2010-02-09 2011-02-16 中国农业科学院农业资源与农业区划研究所 Liquid submerged fermentation culture method for hericium erinaceus
CN102396346A (en) * 2010-02-09 2012-04-04 中国农业科学院农业资源与农业区划研究所 Hericium erinaceus
CN102084782A (en) * 2010-11-30 2011-06-08 忻州市汇恒农林牧综合开发有限公司 Natural planting method of mushroom
CN102690138A (en) * 2012-06-21 2012-09-26 吴利明 Universal lucid ganoderma mycelia fermentation medium combination
CN105474995A (en) * 2015-12-21 2016-04-13 镇江盛弘景观植物有限公司 Cultivation and domestication method of wild collybia albuminosa

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109892169A (en) * 2019-03-28 2019-06-18 上海市农业科学院 A kind of germination method of purple sesame basidiospore germination culture medium and purple sesame basidiospore
CN109892169B (en) * 2019-03-28 2021-06-08 上海市农业科学院 Ganoderma sinensis basidiospore germination culture medium and germination method of ganoderma sinensis basidiospores

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Application publication date: 20171222