CN107047057A - A kind of Antrodia camphorata inoculation method - Google Patents
A kind of Antrodia camphorata inoculation method Download PDFInfo
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- CN107047057A CN107047057A CN201710010058.8A CN201710010058A CN107047057A CN 107047057 A CN107047057 A CN 107047057A CN 201710010058 A CN201710010058 A CN 201710010058A CN 107047057 A CN107047057 A CN 107047057A
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- antrodia camphorata
- cinnamomum
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- kanahirai hay
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The present invention relates to the artificial breeding technique field of Antrodia camphorata, refer in particular to a kind of Antrodia camphorata inoculation method, including linden preparation process, strain fermentation step, cultivate step and harvesting step, the linden preparation process include choosing cinnamomum kanehirai log, stripping and slicing, be exposed to the sun, saline sook and sterilization processing；Strain fermentation step includes implantation and cultivated；The progress that step is cultivated in specific temperature and humidity raising chamber, when Antrodia camphorata fructification when the growing height on Cinnamomum kanahirai hay wooden unit surface is to 2~2.5 centimetres harvest obtaining Antrodia camphorata fructification.The Antrodia camphorata molecular structure triterpenes content for carrying out Antrodia camphorata cultivation using the above method of the present invention reaches the contents level of natural Antrodia camphorata, though overcome the cultivating method triterpenes content of artificial non-camphorwood class close, high-molecular compound mass difference decades of times, drug effect value is lifted at double.
Description
Technical field
The present invention relates to the artificial breeding technique field of Antrodia camphorata, a kind of Antrodia camphorata inoculation method is referred in particular to.
Background technology
Antrodia camphorata also known as cinnamomum kanahirai hay mushroom, belong to Aphyllophorales, porous section, perennial gill fungus mushroom, scientific name for (
Antrodiacamphorata), it is the nineteen ninety just novel species delivered by biochemical boundary.Its growth district is Taiwan mountain area
Between 450~2000 meters of mountain forests of height above sea level, the rotten heart of the peculiar century-old cinnamomum kanehirai trunk above in Taiwan is only grown in
Material inwall, or withered lodging cinnamomum kanehirai timber moist surface, because its collection is difficult, add wild Cinnamomum kanahirai hay wood in recent years by government
Be classified as national treasure level one of biology, also therefore the wooden social event of illegal mining Cinnamomum kanahirai hay still when have what is heard.Because antrodia is in Taiwan
It is considered as unique and precious medicinal fungi, therefore is also current Taiwan with high research and commercial value
The wild fungi of most expensive, is referred to as " refreshing sesame " in Hongkong and Macro, and Taiwan is among the people to be referred to as " ruby in forest ".
At present, cultivation basswood method, plate method, solution fermentation three can be substantially divided into for antrodia artificial culture
Kind.
1st, cultivation basswood method
It is that culture medium cultivates Antrodia camphorata using the original host's cinnamomum kanehirai linden of Antrodia camphorata antrodia；Can obtain it is identical with wild antrodia it
Composition, effect is identical, it is to avoid wild cinnamomum kanehirai illegal logging；But incubation time is up to 2 to three years, toxigenic capacity is high.
2nd, plate method
Antrodia camphorata antrodia strain is subjected to Mycelium culture with space bag.Space includes fibre object, carbohydrate, dairy products
Deng；Similar to wild Antrodia camphorata antrodia profile but different composition, incubation time about three months can be obtained；Toxigenic capacity is high.
3rd, solution fermentation
Using 500 liters even tonne more than liquid fermentation groove carry out Antrodia camphorata antrodia strain liquid and ferment to collect mycelia
Body；Incubation time is short, and about seven to fortnight or so；But the distinctive triterpenes of wild antrodia can not be obtained.
, there is following weak point in three kinds of breeding methods in summary：
1st, prior art cultivates the product suitable with wild Antrodia camphorata quality, must use Cinnamomum kanahirai hay carpentery workshop to cultivate bacterium wood.But ox
Camphor tree has been classified as Taiwan rare tree species (first class of protection), and raw material sources are very difficult, and the planting conditions of cinnamomum kanehirai
It is required that higher, growth cycle is long, and industrialization is cultivated and is restricted.
2nd, prior art uses other culture mediums (being raw material as bacterium bag as use the young dregs of rice of cotton, corncob and weed tree sawdust) to train
The Antrodia camphorata mushroom entity triterpenes content brought out is extremely low, and drug effect value is low.
The content of the invention
The high yield Antrodia camphorata cultivation that artificial batch is cultivated can be realized the technical problem to be solved in the present invention is to provide a kind of
Method, has the advantages that the cultivation cycle is short, survival rate is high, active component is abundant.
In order to solve the above-mentioned technical problem, the present invention is adopted the following technical scheme that：
A kind of Antrodia camphorata inoculation method, including linden preparation process, strain fermentation step, cultivation step and harvesting step, it is special
Levy and be：
The linden preparation process includes：
Step A1, chooses cinnamomum kanehirai log, and cinnamomum kanehirai log is cut into the Cinnamomum kanahirai hay wooden unit that blocking footpath size is 25~45cm；
Step A2, heavy natural sunning is carried out after polishing the surface of Cinnamomum kanahirai hay wooden unit；
Soaked 24~96 hours in step A3, the Cinnamomum kanahirai hay wooden unit immersion salt solution after being exposed to the sun；
Cinnamomum kanahirai hay wooden unit after immersion, is first put into 105~115 DEG C of environment and carries out sterilization processing by step A4, then the Cinnamomum kanahirai hay is wooden
Block is put into progress sterilization cooling treatment in UV environment；
The strain fermentation step includes：
Step B1, puts nutrient solution into ware training, then the strain mixed liquor with Antrodia camphorata fructification is put into ware in desinfection chamber
Training；
Step B2, the ware training in step B1 is placed in 12~26 DEG C of environment and keeps obtaining Antrodia camphorata strain in 3~6 months；
The cultivation step includes：
Step C1, raising chamber is transferred to by the Cinnamomum kanahirai hay wooden unit that step A4 is obtained, then the Antrodia camphorata strain that step B2 is obtained is transplanted
Onto Cinnamomum kanahirai hay wooden unit；
Step C2, it is 12~35 DEG C to keep the temperature in raising chamber, and humidity is 50~98%, and raising chamber keeps ventilation state；
The harvesting step includes：When Antrodia camphorata fructification is carried out when the growing height on Cinnamomum kanahirai hay wooden unit surface is to 2~2.5 centimetres
Harvesting obtains Antrodia camphorata fructification.
It is preferred that, the strain mixed liquor in the step B1 includes according to percentage by weight
The fructification extract solution 0.04%~0.06% of Antrodia camphorata,
Potato extract solution 1.2%~1.7%,
Albumen 0.35%~0.42%,
Benevolence of anticipating extract solution 0.025%~0.035%,
Wheat skin extract solution 0.018%~0.021%,
Agar extract solution 0.025%~0.035%,
Remaining is water.
It is further preferred that the strain mixed liquor in the step B1 includes according to percentage by weight：
The fructification extract solution 0.05% of Antrodia camphorata, potato extract solution 1.5%, albumen 0.4%, meaning benevolence extract solution 0.03%, wheat
Skin extract solution 0.02%, agar extract solution 0.03%, remaining is water.
It is preferred that, it is 60~80 hours to the heavy natural sunning time of Cinnamomum kanahirai hay wooden unit in the step A2.
It is preferred that, it is 5.5~6.5 hours to the ultraviolet sterilization time of Cinnamomum kanahirai hay wooden unit in the step A4.
It is preferred that, the cultivation time in the step C2 in raising chamber is 10~13 months.
It is preferred that, in the step C2, the Cinnamomum kanahirai hay wooden unit for being periodically implanted with Antrodia camphorata strain to kind carries out atomized water spray, sprays twice
Time interval between water smoke is 40~55 hours.
It is preferred that, after the harvesting step terminates, the Cinnamomum kanahirai hay wooden unit surface after harvesting is polished again, polished
Thickness is 0.8~1.2cm, repeat step A4 after polishing.
It is preferred that, the cinnamomum kanehirai log is derived from the pure natural wild cinnamomum kanehirai linden in Taiwan Alishan.
The beneficial effects of the present invention are：
The invention provides a kind of Antrodia camphorata inoculation method, the Antrodia camphorata of Antrodia camphorata cultivation is carried out using the above method of the present invention
Molecular structure triterpenes content reaches the contents level of natural Antrodia camphorata, though overcome the cultivating method triterpenes of artificial non-camphorwood class to contain
Close, high-molecular compound mass difference decades of times is measured, drug effect value is lifted at double.
After the harvesting step terminates, the Cinnamomum kanahirai hay wooden unit surface after harvesting is polished again, polishing thickness is
Repeat step A4 after 0.8~1.2cm, polishing, in order to which Cinnamomum kanahirai hay wooden unit is recycled.Improve the repetition of Cinnamomum kanahirai hay wood
Using number of times, on the premise of Antrodia camphorata yield is constant, the usage amount to cinnamomum kanehirai log is greatly reduced, cinnamomum kanehirai log is utilized
Rate and the value of environmental protection are higher.
Embodiment
For the ease of the understanding of those skilled in the art, with reference to embodiment, the present invention is further illustrated, real
The content that the mode of applying is referred to not limitation of the invention.
A kind of embodiment one of Antrodia camphorata inoculation method, including linden preparation process, strain fermentation step, cultivate step and
Step is harvested, wherein linden preparation process includes：
Step A1, chooses cinnamomum kanehirai log, and cinnamomum kanehirai log is derived from the pure natural wild cinnamomum kanehirai linden in Taiwan Alishan
Wood, the Cinnamomum kanahirai hay wooden unit that blocking footpath size is 25~45cm is cut by cinnamomum kanehirai log；
Step A2, carries out heavy natural sunning after polishing the surface of Cinnamomum kanahirai hay wooden unit, the heavy natural sunning time is 60~80 hours；
Soaked 24~96 hours in step A3, the Cinnamomum kanahirai hay wooden unit immersion salt solution after being exposed to the sun；
Cinnamomum kanahirai hay wooden unit after immersion, is first put into 115 DEG C of environment and carries out sterilization processing, then the Cinnamomum kanahirai hay wooden unit is put into by step A4
Sterilization cooling treatment is carried out in UV environment, the ultraviolet sterilization time is 5.5 hours；
The strain fermentation step includes：
Step B1, puts nutrient solution into ware training, then the strain mixed liquor with Antrodia camphorata fructification is put into ware in desinfection chamber
Training, strain mixed liquor includes the fructification extract solution 0.04% of Antrodia camphorata, potato extract solution 1.7%, egg according to percentage by weight
White 0.35%, meaning benevolence extract solution 0.035%, wheat skin extract solution 0.021%, agar extract solution 0.025%, remaining is water.
Step B2, the ware training in step B1 is placed in 12 DEG C of environment and keeps obtaining within 6 months Antrodia camphorata strain；
The cultivation step includes：
Step C1, raising chamber is transferred to by the Cinnamomum kanahirai hay wooden unit that step A4 is obtained, then the Antrodia camphorata strain that step B2 is obtained is transplanted
Onto Cinnamomum kanahirai hay wooden unit；
Step C2, it is 12~35 DEG C to keep the temperature in raising chamber, and humidity is 50~98%, and raising chamber keeps ventilation state, in training
The cultivation time in brood chamber is that the Cinnamomum kanahirai hay wooden unit for being periodically implanted with Antrodia camphorata strain to kind in 10~13 months, cultivation time is sprayed
Water smoke, the time interval between atomized water spray is 40~55 hours twice；
The harvesting step includes：When Antrodia camphorata fructification is carried out when the growing height on Cinnamomum kanahirai hay wooden unit surface is to 2~2.5 centimetres
Harvesting obtains Antrodia camphorata fructification.
The Antrodia camphorata molecular structure triterpenes content for carrying out Antrodia camphorata cultivation using the above method of the present invention reaches naturally
The contents level of Antrodia camphorata, though overcome the cultivating method triterpenes content of artificial non-camphorwood class close, high-molecular compound molecular weight
Decades of times is differed, drug effect value is lifted at double.
In the present embodiment, after the harvesting step terminates, the Cinnamomum kanahirai hay wooden unit surface after harvesting is polished again,
Polishing thickness is 0.8~1.2cm, repeat step A4 after polishing, in order to which Cinnamomum kanahirai hay wooden unit is recycled.Improve ox
The recycling number of times of camphorwood, on the premise of Antrodia camphorata yield is constant, greatly reduces the usage amount to cinnamomum kanehirai log, Cinnamomum kanahirai hay
Set log utilization rate and the value of environmental protection is higher.
A kind of embodiment two of Antrodia camphorata inoculation method, is with the difference of above-described embodiment one：Strain mixed liquor
Include the fructification extract solution 0.06% of Antrodia camphorata according to percentage by weight, potato extract solution 1.2%, albumen 0.35%~
0.42%, meaning benevolence extract solution 0.025%, wheat skin extract solution 0.018%, agar extract solution 0.035%, remaining is water.
Cinnamomum kanahirai hay wooden unit after immersion, is first put into 105 DEG C of environment and carries out sterilization processing by the step A4 of the present embodiment, then will
The Cinnamomum kanahirai hay wooden unit is put into progress sterilization cooling treatment in UV environment, and the ultraviolet sterilization time is 6.5 hours；In step B2,
Ware training in step B1 is placed in 26 DEG C of environment and keeps obtaining within 3 months Antrodia camphorata strain.
The effect of the Antrodia camphorata fructification that the present embodiment Antrodia camphorata inoculation method is nurtured in terms of liver protecting is more prominent,
Especially for liver has the patient of chronic disease, health-care effect is more notable.
A kind of embodiment three of Antrodia camphorata inoculation method, including linden preparation process, strain fermentation step, cultivate step and
Step is harvested, wherein linden preparation process includes：
Step A1, chooses cinnamomum kanehirai log, and cinnamomum kanehirai log is derived from the pure natural wild cinnamomum kanehirai linden in Taiwan Alishan
Wood, the Cinnamomum kanahirai hay wooden unit that blocking footpath size is 30 × 40cm is cut by cinnamomum kanehirai log；
Step A2, carries out heavy natural sunning after polishing the surface of Cinnamomum kanahirai hay wooden unit, the heavy natural sunning time is 72 hours；
Soaked 60 hours in step A3, the Cinnamomum kanahirai hay wooden unit immersion salt solution after being exposed to the sun；
Cinnamomum kanahirai hay wooden unit after immersion, is first put into 110 DEG C of environment and carries out sterilization processing, then the Cinnamomum kanahirai hay wooden unit is put into by step A4
Sterilization cooling treatment is carried out in UV environment, the ultraviolet sterilization time is 6 hours；
The strain fermentation step includes：
Step B1, puts nutrient solution into ware training, then the strain mixed liquor with Antrodia camphorata fructification is put into ware in desinfection chamber
Training, strain mixed liquor includes the fructification extract solution 0.05% of Antrodia camphorata, potato extract solution 1.5%, egg according to percentage by weight
White 0.4%, meaning benevolence extract solution 0.03%, wheat skin extract solution 0.02%, agar extract solution 0.03%, remaining is water.
Step B2, the ware training in step B1 is placed in 18 DEG C of environment and keeps obtaining within 5 months Antrodia camphorata strain；
The cultivation step includes：
Step C1, raising chamber is transferred to by the Cinnamomum kanahirai hay wooden unit that step A4 is obtained, then the Antrodia camphorata strain that step B2 is obtained is transplanted
Onto Cinnamomum kanahirai hay wooden unit；
Step C2, it is 26 DEG C to keep the temperature in raising chamber, and humidity is 70%, and raising chamber keeps ventilation state, in raising chamber
The cultivation time is that the Cinnamomum kanahirai hay wooden unit for being periodically implanted with Antrodia camphorata strain to kind in 12 months, cultivation time carries out atomized water spray, is sprayed twice
Time interval between water smoke is 48 hours；
The harvesting step includes：When Antrodia camphorata fructification is carried out when the growing height on Cinnamomum kanahirai hay wooden unit surface is to 2~2.5 centimetres
Harvesting obtains Antrodia camphorata fructification.
The Antrodia camphorata molecular structure triterpenes content for carrying out Antrodia camphorata cultivation using the above method of the present invention reaches naturally
The contents level of Antrodia camphorata, though overcome the cultivating method triterpenes content of artificial non-camphorwood class close, high-molecular compound molecular weight
Decades of times is differed, but drug effect value is lifted at double.
In addition, the work(of the Antrodia camphorata fructification that nurtures of the present embodiment Antrodia camphorata inoculation method not only in terms of liver protecting
Effect is more prominent, also has good therapeutic effect in terms of its antitumaous effect and suppression gastric epithelial cell inflammation, medicinal effects are more prominent,
Comprehensive health-care effect is more notable, and practicality is stronger.
Embodiment described above only expresses some embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (9)
1. a kind of Antrodia camphorata inoculation method, including linden preparation process, strain fermentation step, cultivation step and harvesting step, its
It is characterised by：
The linden preparation process includes：
Step A1, chooses cinnamomum kanehirai log, and cinnamomum kanehirai log is cut into the Cinnamomum kanahirai hay wooden unit that blocking footpath size is 25~45cm；
Step A2, heavy natural sunning is carried out after polishing the surface of Cinnamomum kanahirai hay wooden unit；
Soaked 24~96 hours in step A3, the Cinnamomum kanahirai hay wooden unit immersion salt solution after being exposed to the sun；
Cinnamomum kanahirai hay wooden unit after immersion, is first put into 105~115 DEG C of environment and carries out sterilization processing by step A4, then the Cinnamomum kanahirai hay is wooden
Block is put into progress sterilization cooling treatment in UV environment；
The strain fermentation step includes：
Step B1, puts nutrient solution into ware training, then the strain mixed liquor with Antrodia camphorata fructification is put into ware in desinfection chamber
Training；
Step B2, the ware training in step B1 is placed in 12~26 DEG C of environment and keeps obtaining Antrodia camphorata strain in 3~6 months；
The cultivation step includes：
Step C1, raising chamber is transferred to by the Cinnamomum kanahirai hay wooden unit that step A4 is obtained, then the Antrodia camphorata strain that step B2 is obtained is transplanted
Onto Cinnamomum kanahirai hay wooden unit；
Step C2, it is 12~35 DEG C to keep the temperature in raising chamber, and humidity is 50~98%, and raising chamber keeps ventilation state；
The harvesting step includes：When Antrodia camphorata fructification is carried out when the growing height on Cinnamomum kanahirai hay wooden unit surface is to 2~2.5 centimetres
Harvesting obtains Antrodia camphorata fructification.
2. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：Strain mixed liquor in the step B1 is pressed
Include according to percentage by weight
The fructification extract solution 0.04%~0.06% of Antrodia camphorata,
Potato extract solution 1.2%~1.7%,
Albumen 0.35%~0.42%,
Benevolence of anticipating extract solution 0.025%~0.035%,
Wheat skin extract solution 0.018%~0.021%,
Agar extract solution 0.025%~0.035%,
Remaining is water.
3. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：Strain mixed liquor in the step B1 is pressed
Include according to percentage by weight：The fructification extract solution 0.05% of Antrodia camphorata, potato extract solution 1.5%, albumen 0.4%, meaning benevolence is carried
Take liquid 0.03%, wheat skin extract solution 0.02%, agar extract solution 0.03%, remaining is water.
4. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：In the step A2 to Cinnamomum kanahirai hay wooden unit from
The time be so exposed to the sun for 60~80 hours.
5. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：To the purple of Cinnamomum kanahirai hay wooden unit in the step A4
Outside line sterilizing time is 5.5~6.5 hours.
6. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：Training in the step C2 in raising chamber
The time is educated for 10~13 months.
7. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：In the step C2, periodically it is implanted with to kind
The Cinnamomum kanahirai hay wooden unit of Antrodia camphorata strain carries out atomized water spray, and the time interval between atomized water spray is 40~55 hours twice.
8. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：It is again right after the harvesting step terminates
Cinnamomum kanahirai hay wooden unit surface after harvesting is polished, and polishing thickness is 0.8~1.2cm, repeat step A4 after polishing.
9. Antrodia camphorata inoculation method according to claim 1, it is characterised in that：The cinnamomum kanehirai log is derived from TaiWan, China
The regional pure natural wild cinnamomum kanehirai linden in Alishan.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110896783A (en) * | 2018-09-18 | 2020-03-24 | 广东东阳光药业有限公司 | Culture medium and Antrodia camphorata fruiting body cultivation method |
| CN112021066A (en) * | 2020-09-10 | 2020-12-04 | 宁波峤锋生物科技有限公司 | Antrodia camphorata cultivation method |
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| TWM420978U (en) * | 2011-07-14 | 2012-01-21 | bao-yu Guo | Device for cultivating antrodia camphorata using basswood |
| TW201238469A (en) * | 2011-03-31 | 2012-10-01 | Wei-Chih Chang | Method for innoculating and cultivating antrodia camphorate |
| CN103918472A (en) * | 2013-01-15 | 2014-07-16 | 漳州美利德生物工程有限公司 | Antrodia camphorata basswood cultivation method |
| CN105145112A (en) * | 2015-07-08 | 2015-12-16 | 范春娟 | Method for cultivating antrodia sporocarp by using cinnamomum camphora cut-log |
| CN105557294A (en) * | 2014-10-14 | 2016-05-11 | 芝圣（天津）生物科技有限公司 | Artificial culture method for Antrodia camphorata by utilizing basswood |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201238469A (en) * | 2011-03-31 | 2012-10-01 | Wei-Chih Chang | Method for innoculating and cultivating antrodia camphorate |
| TWM420978U (en) * | 2011-07-14 | 2012-01-21 | bao-yu Guo | Device for cultivating antrodia camphorata using basswood |
| CN103918472A (en) * | 2013-01-15 | 2014-07-16 | 漳州美利德生物工程有限公司 | Antrodia camphorata basswood cultivation method |
| CN105557294A (en) * | 2014-10-14 | 2016-05-11 | 芝圣（天津）生物科技有限公司 | Artificial culture method for Antrodia camphorata by utilizing basswood |
| CN105145112A (en) * | 2015-07-08 | 2015-12-16 | 范春娟 | Method for cultivating antrodia sporocarp by using cinnamomum camphora cut-log |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110896783A (en) * | 2018-09-18 | 2020-03-24 | 广东东阳光药业有限公司 | Culture medium and Antrodia camphorata fruiting body cultivation method |
| CN112021066A (en) * | 2020-09-10 | 2020-12-04 | 宁波峤锋生物科技有限公司 | Antrodia camphorata cultivation method |
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