CN105557294A - Artificial culture method for Antrodia camphorata by utilizing basswood - Google Patents

Abstract

The invention provides a high-production-yield artificial culture method for Antrodia camphorata by utilizing basswood. The process flow of the method comprises the following procedures: preparation of a fungus growth nutrient solution, basswood selection, cutting of the basswood into sections, hole drilling of the basswood, soaking of the basswood in the fungus growth nutrient solution, basswood stacking, bag sealing of the basswood, basswood sterilization, basswood inoculation, Antrodia camphorata culturing, and Antrodia camphorata germination and harvesting. According to the invention, the procedure of sterilization operation is adopted in the process of culture, so fungi in the basswood are eradicated, and the influence on the growth of Antrodia camphorata due to the existence of a variety of fungi in the basswood is avoided; the procedure of basswood selection is adopted, and an original Antrodia camphorata host--Cinnamomum kanehirai Hay basswood is selected as a culture medium for culture of Antrodia camphorata, so late-term growth of Antrodia camphorata is guaranteed; and the procedure of Antrodia camphorata culture is divided into two stages, wherein a first stage is realized in a culture room, while a second culture stage is realized in a culture box, and different temperatures and humidities are respectively set in the culture room and the culture box, so the demands for temperatures and humidities in different growth stages of Antrodia camphorata are met.

Description

A kind of method utilizing linden artificial culture Antrodia camphorata

Technical field

The present invention relates to Antrodia camphorata and cultivate field, particularly relate to a kind of method utilizing linden artificial culture Antrodia camphorata.

Background technology

Malignant tumour is the disease of a class serious harm human health and life.According to World Health Organization's statistics, within 2012, nearly more than 1,200 ten thousand people in the whole world suffer from cancer, and wherein more than 850 ten thousand people die from this disease.Meanwhile, newfound patient just with annual 1800000 speed increase.China's third time human mortality's reason sample survey results display: malignant tumour has become the first place of China city dweller cause of death, accounts for 25% of all dead sums.In recent years, the basis such as oncology epidemiology, pathogenesis, clinical diagnosis and treatment and clinicing aspect etc. all achieve greater advance, filter out multiple antineoplastic.But oneself the antineoplastic ubiquity therapeutic efficiency that has is low at present, poor selectivity, toxicity large and the outstanding problem such as oncocyte resistance.Therefore, from natural animal and plant, find the anticancer active constituent that toxicity is low, curative effect is high and just become one of focus of scientific worker's research both at home and abroad in recent years.

Research in recent years proves, the advantage that Chinese medicine cancer has efficiently, low toxicity, selectivity are strong.In the therapeutic process of cancer, there is definite curative effect, show as and improve clinical symptoms, extend life cycle, improve the quality of living, control pain, prevent the aspects such as recurrence, with Chemotherapy plus, still have the effect of Synergy and attenuation.Existing Chinese herbal medicine traditional is in a large number proved has cancer suppressing action, presses down cancer and blood vessel formation against function etc. as Artemether.At present, the cancer therapy drug deriving from plant has accounted for about 32% of total cancer therapy drug, and wherein terpenoid accounts for significant proportion.

Antrodia camphorata (Antrodiacamphorata) has another name called Cinnamomum kanahirai hay mushroom, camphor tree mushroom or camphor tree sesame, and being the novel species just delivered by biochemical boundary the nineties in 20th century, is the distinctive fungi in Taiwan.Antrodia camphorata contains many physiologically active ingredients, as polysaccharide body, triterpene compound, Sudismase, adenosine, protein (containing immune protein), vitamin, trace element, nucleic acid, agglutinin, amino acid, steroid, cellulose, blood pressure stabilization material etc., but topmost physiologically active ingredient is polysaccharide (candy gathers in β-D-Portugal) and triterpene compound.Antrodia camphorata gas fragrance, taste is arduous, flat, pharmacodynamics has promoting the circulation of qi of dispeling the wind, changes that silt is invigorated blood circulation, the knot that disappears in temperature, removing toxicity for detumescence, sedation-analgesia, antibacterial, antiviral, antitumor, lifting immunity of organisms effect, and to have no side effect completely; More unique function is had for treatment gastrointestinal distress, diarrhea and vomiting, food poisoning, mushroom poisoning, diabetes, alcoholic liver, fatty liver, cirrhosis, liver cancer etc.But Antrodia camphorata is generally wild plant, but wild quantity can not meet the demand of people to it, so people propagate artificially Antrodia camphorata, but propagating artificially now yields poorly, and aquaculture cost is high.

Summary of the invention

For solving the problem, invention provides a kind of and produces the high method utilizing linden artificial culture Antrodia camphorata of output, it is characterized in that the technological process of production comprises:

Produce fungus grown nutrient solution:

Antrodia camphorata strain fermentation is used to produce Antrodia camphorata fungus grown nutrient solution;

Linden is selected:

Select Antrodia camphorata original host's Cinnamomum kanahirai hay tree linden to be medium cultivation Antrodia camphorata, described linden is purebred Antrodia camphorata linden;

Linden segment:

The linden chosen is carried out segment process;

Linden is holed:

By the conscientious boring of linden cut, for subsequent use for implanting bacterial classification;

Linden fungus grown nutrient solution soaks:

Hole-drilled linden is put into fungus grown nutrient solution soak, linden is soaked into completely in fungus grown nutrient solution during immersion, soaking temperature 15-20 degree, soak time 24-40 hour;

Linden is banked up:

Linden after soaking is carried out composting, fungus grown nutrition hydraulic control is done, and provide the sufficient time to make fungus grown nutrient solution infiltrate in linden, composting time 24-36 hour;

Linden envelope:

Linden after composting is loaded in freshness protection package, makes it ensure wetness, be convenient to Antrodia camphorata growth;

Linden sterilizing:

Packaged linden is put into sterilizer and carries out sterilizing, avoid in linden, there is multiple mushroom, affect Antrodia camphorata growth;

Linden is inoculated:

Linden after sterilizing is opened envelope and carries out inoculation operation, bacterination is implanted in linden boring;

Antrodia camphorata is cultivated:

After vaccine is implanted, linden is sealed and puts into culturing room and cultivate, when the mycelia color that grows out changes, every block linden is put into single incubator cultivate, in incubator, temperature ensures at 15-20 DEG C, and humidity is 30-50%, guarantees the growing environment of Antrodia camphorata;

Go out sesame to gather:

When taking it after Antrodia camphorata production maturation.

Beneficial effect of the present invention is: the culture conditions that present invention optimizes Antrodia camphorata, realizes the extensive artificial of Antrodia camphorata and cultivates, for exploitation Antrodia camphorata food (medicine) product provide technical support.The present invention have employed disinfecting action in cultivating process, is all eliminated by the mushroom in linden, avoids there is multiple mushroom in linden, affects Antrodia camphorata growth; The present invention have chosen Antrodia camphorata original host's Cinnamomum kanahirai hay tree linden when carrying out linden and choosing be medium cultivation Antrodia camphorata, ensure that the growth of later stage Antrodia camphorata, the present invention is when carrying out Antrodia camphorata and cultivating, be divided into two benches, first stage is in culturing room, second stage is in incubator, and culturing room and incubator are respectively equipped with different temperature and humidities, meets the demand of Antrodia camphorata Different growth phases to temperature and humidity.

Accompanying drawing explanation

Fig. 1 is flowage structure schematic diagram of the present invention.

Embodiment

In order to make those skilled in the art person better understand technical solution of the present invention, below in conjunction with drawings and embodiments, the present invention is described in further detail.

embodiment 1

Antrodia camphorata cultivates flow process:

First Antrodia camphorata strain fermentation is used to produce Antrodia camphorata fungus grown nutrient solution; Select Antrodia camphorata original host's Cinnamomum kanahirai hay tree linden to be medium cultivation Antrodia camphorata, described linden is purebred Antrodia camphorata linden; The linden chosen is carried out segment process; By the conscientious boring of linden cut, for subsequent use for implanting bacterial classification; Hole-drilled linden is put into fungus grown nutrient solution soak, linden is soaked into completely in fungus grown nutrient solution during immersion, soaking temperature 15-20 degree, soak time 24-40 hour; Linden after soaking is carried out composting, fungus grown nutrition hydraulic control is done, and provide the sufficient time to make fungus grown nutrient solution infiltrate in linden, composting time 24-36 hour; Linden after composting is loaded in freshness protection package, makes it ensure wetness, be convenient to Antrodia camphorata growth;

Packaged linden is put into sterilizer and carries out sterilizing, avoid in linden, there is multiple mushroom, affect Antrodia camphorata growth; Linden after sterilizing is opened envelope and carries out inoculation operation, bacterination is implanted in linden boring;

After vaccine is implanted, linden is sealed and puts into culturing room and cultivate, when the mycelia color that grows out changes, every block linden is put into single incubator cultivate, in incubator, temperature ensures at 15-20 DEG C, and humidity is 30-50%, guarantees the growing environment of Antrodia camphorata; When taking it after Antrodia camphorata production maturation.

The above is only the preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvement, and these improvement also should be considered as protection scope of the present invention.

Claims (1)

1. utilize a method for linden artificial culture Antrodia camphorata, it is characterized in that the technological process of production comprises:

Produce fungus grown nutrient solution:

Antrodia camphorata strain fermentation is used to produce Antrodia camphorata fungus grown nutrient solution;

Linden is selected:

Select Antrodia camphorata original host's Cinnamomum kanahirai hay tree linden to be medium cultivation Antrodia camphorata, described linden is purebred Antrodia camphorata linden;

Linden segment:

The linden chosen is carried out segment process;

Linden is holed:

By the conscientious boring of linden cut, for subsequent use for implanting bacterial classification;

Linden fungus grown nutrient solution soaks:

Hole-drilled linden is put into fungus grown nutrient solution soak, linden is soaked into completely in fungus grown nutrient solution during immersion, soaking temperature 15-20 degree, soak time 24-40 hour;

Linden is banked up:

Linden after soaking is carried out composting, fungus grown nutrition hydraulic control is done, and provide the sufficient time to make fungus grown nutrient solution infiltrate in linden, composting time 24-36 hour;

Linden envelope:

Linden after composting is loaded in freshness protection package, makes it ensure wetness, be convenient to Antrodia camphorata growth;

Linden sterilizing:

Packaged linden is put into sterilizer and carries out sterilizing, avoid in linden, there is multiple mushroom, affect Antrodia camphorata growth;

Linden is inoculated:

Linden after sterilizing is opened envelope and carries out inoculation operation, bacterination is implanted in linden boring;

Antrodia camphorata is cultivated:

After vaccine is implanted, linden is sealed and puts into culturing room and cultivate, when the mycelia color that grows out changes, every block linden is put into single incubator cultivate, in incubator, temperature ensures at 15-20 DEG C, and humidity is 30-50%, guarantees the growing environment of Antrodia camphorata;

Go out sesame to gather:

When taking it after Antrodia camphorata production maturation.