CN1117524C - Method for producing biologic inorganic composite feed additive for animals and fowls - Google Patents
Method for producing biologic inorganic composite feed additive for animals and fowls Download PDFInfo
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- CN1117524C CN1117524C CN97125739A CN97125739A CN1117524C CN 1117524 C CN1117524 C CN 1117524C CN 97125739 A CN97125739 A CN 97125739A CN 97125739 A CN97125739 A CN 97125739A CN 1117524 C CN1117524 C CN 1117524C
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Abstract
The present invention relates to a method for preparing an additive of poultry and fowl feed by adopting organism compound enzymes, trace elements, vitamins, mineral substances, amino acid, carriers, etc. The additive generates mutual synergistic effect and comprehensive balance action on animals by mixing multi-bacterium organism compound enzymes, reasonable trace elements, vitamins, mineral substances, amino acid, etc. by the action of the multi-bacterium organism compound enzymes and other substances. The utilization rate of feed is fully utilized to enhance the absorption and the conversion of animals to the feed, and thereby, the purposes of promoting the growth of the animals and saving the feed are achieved. The present invention has the advantages of easy control of the preparation method and the preparation technology, little investment, low cost and obvious efficiency, is suitable for medium and small-sized enterprises and is particularly suitable for investment and plant establishment pf township enterprises.
Description
The present invention relates to a kind of production method of biologic inorganic composite feed additive for animals and fowls, belong to a kind of method of using many bacterium biology, raw material such as inorganic to cooperate, in raw materials such as inorganic raw material, trace element, multiannensional-sine, add biological complex enzyme, the short long agent of preferred optimum formula and interpolation animal, biology enzyme is being produced and processed and inorganic raw material etc. is being carried out on the pretreated basis, finally producing the composite feed additive that suitable growth of animals or poultry needs.
Have many pieces of patent documents to disclose all methods of utilizing production composite feed additives such as biology enzyme trace element in the prior art, wherein immediate documents is:
Utilize multiple kinds of crops by-product production feed complex enzyme 90105141.4 the application number Chinese invention patent discloses, its shortcoming is: the one, and bake out temperature is higher, and enzyme loss alive is bigger, and bake out temperature should be controlled at below 60 ℃; The 2nd, the complex enzyme product of being produced is not disclosed in the amount that adds in the feed, therefore, can't determine addition in feed adds; The 3rd, add complex enzyme merely, be difficult to make feed to have the effect of overall balance animal body, growth because growth of animal need be not only biology enzyme, the more important thing is mineral matter and various trace elements.
Utilize several mineral materials, trace element, short long agent, Chinese herbal medicine etc. to cooperate production feed addictive, its shortcoming 95111417.4 Chinese invention patent discloses: the one, the preliminary treatment of raw material is not open, is difficult to implement according to the scheme that this patent proposed; The 2nd, though this patent is relatively more comprehensive on raw material, from raw material, all be to buy from the market, therefore, raw material does not have too big advantage on price.
Utilize mineral matter, trace element etc. to be combined into feed addictive, its shortcoming 94112859.8 Chinese invention patent discloses: the one, the ratio that additive adds in the feed is big, has increased the cost of feed; The 2nd, raw material embraces a wide spectrum of ideas, and does not have new meaning aspect technical scheme.
China is a populous country, solving human survival is current principal contradiction with satisfying living needs, China is a large agricultural country in addition, aquaculture is very flourishing, therefore, produce and make composite feed additive and be applied to animal husbandry, have profound significance for the living standard that promotes growth of animal, saves food, improves the people.
The production method that the objective of the invention is to overcome the prior art deficiency and a kind of biologic inorganic composite feed additive for animals and fowls is provided, this method is used biological complex enzyme and mineral matter, trace element, short long the synergy polynary the cooperation mutually of agent, and it is good to make it resultant effect; Adopt solid fermentation method in the complex enzyme production process, it is few to make it equipment investment; In complex enzyme product drying course, strict temperature is controlled at about 50 ℃, has effectively protected the activity of complex enzyme; Aspect pretreatment of raw material, satisfy of the dispersion of each effective ingredient as possible, to make full use of each active ingredient and optimum efficiency at carrier.
The production method of biologic inorganic composite feed additive for animals and fowls of the present invention comprises following step:
1) preparation of raw material and preliminary treatment comprise the preparation of biological complex enzyme bacterial classification and the production of product, the separation of bacterial classification, purifying, mutagenesis;
2) prescription and the preparation of inorganic, mineral matter, trace element;
3) preliminary treatment of biologic inorganic composite feed additive:
(1) being prepared as of biological complex enzyme preparation:
1. the preparation of fungi space left side aspergillus IFFI2378
Culture medium: soya-bean cake powder 60%, wheat wheat bran 35%, corn flour 4%
Na
4Cl0.5% CaCl0.3%, Na
2HPO
40.2%, insert second class inoculum 1%, the conventional cultivation of solid sterilization is pressed in fermentation, and incubation time is about 48 hours.
2. the preparation of the U.S. aspergillus IFFI2379 in a fungi space left side
Culture medium: soya-bean cake powder 60%, wheat wheat bran 35%, corn flour 4%, Na
4Cl0.5% CaCl0.3%, Na
2HPO
40.2%, insert second class inoculum 1%, the conventional cultivation of solid sterilization is pressed in fermentation, and incubation time is about 48 hours.
3. the preparation of fungi aspergillus awamori IFFI2194
Culture medium: wheat wheat bran 85%, corn flour 5%, soya-bean cake 5%, powder of straw 5% inserts second class inoculum 1%, and the conventional cultivation of solid sterilization is pressed in fermentation, and incubation time is about 48 hours.
4. the preparation of aspergillus niger IFFI2447
Culture medium: wheat wheat bran 80%, soya-bean cake 10%, corn flour 5%, starch 3%, sugar 1%, NaNO
30.2%, MgSO
40.05%, K
2HPO
40.1%, KCl0.05%, feSO
40.01%, insert second class inoculum 1%, the conventional cultivation of solid sterilization is pressed in fermentation, and incubation time is about 48 hours.
5. the preparation of aspergillus niger IFFI2214
Culture medium: soya-bean cake powder 10%, wheat wheat bran 82%, corn flour 5%, pomace 2%, (NH) SO
40.5%, insert second class inoculum 1%, the conventional cultivation of solid sterilization is pressed in fermentation, and incubation time is about 48 hours.
6. the preparation of fungi Trichoderma viride AS3.3032
Culture medium: wheat wheat bran 30%, corn flour 10%, powder of straw 59%, urea 0.3%, ammonium sulfate 0.3% inserts second class inoculum 1%, and the conventional cultivation of solid sterilization is pressed in fermentation, and incubation time is about 48 hours.
7. the preparation of fungi Trichoderma viride AS3.2064
Culture medium: wheat wheat bran 30%, corn flour 10%, powder of straw 59%, urea 0.3%, ammonium sulfate 0.3% inserts second class inoculum 1%, and the conventional cultivation of solid sterilization is pressed in fermentation, and incubation time is about 48 hours.
Insert dryer after the fermentation of above biological enzyme formulation or, pulverized the 30-40 order, add and package spare after an amount of stabilizing agent (saltcake, sodium chloride, zeolite powder etc.) mixes according to quantity with the local method oven dry.
(2) prescription of raw material such as inorganic, mineral matter, trace element
The plain 0.6-1.4% of compound vitamin, ferrous sulfate 1.3-2%, manganese sulfate 0.2-0.5%, copper sulphate 1-2%, zinc sulfate 1.5-2%, KI 0.002-0.003%, cobalt chloride 0.007-0.01%, sodium selenite 0.002-0.003%, olaquindox 0.3-0.6%, boric acid 0.06-0.1%, methionine 5%, lysine 5%, complex enzyme 10-15%, lightweight charcoal acid calcium 35-40%, calcium monohydrogen phosphate 40-50%
(3) preliminary treatment of raw material
Trace element should dryly be handled earlier, pulverizes 100 orders, and denier compositions such as cobalt, iodine, selenium will be pulverized 200 orders, will use the ball mill fine grinding in case of necessity, mixed to reach the best.Present technique invention biological complex enzyme has proposed the scheme and the goods of raw materials for production, and other raw material is all bought from market, therefore, buys raw material and will ensure the quality of products.
(4) preparation mixes
Earlier add carrier calcium monohydrogen phosphate and lightweight charcoal acid calcium in the mixer according to quantity, add grease according to quantity, starting mixer stirred 5 minutes, thereafter pretreated trace element, auxin etc. are added according to quantity and stirred 5-10 minute, then complex enzyme, multiannensional-sine, amino acid are added in the mixer according to quantity, start mixer and stirred 10-15 minute, the weighing packing, the product of making is 1% feed addictive,, should add this agent 1kg in the 100kg feed that is.
In the present invention, the raw material of biological complex enzyme is the leftover bits and pieces of agricultural byproducts, therefore, it is lower as its cost of material of feed addictive to produce complex enzyme, has good exploitation and prospect of production, biological complex enzyme adds in the feed complementary with other trace element, promoted the metabolic function of animal, can improve the utilization rate of animal greatly, improved the condition that animal is assimilated feed by external interpolation enzyme, reach and save food, promote the effect that livestock and poultry increase.Wherein, a fungi space left side U.S. aspergillus IFFI2378 and 2379 is an acid protease, can promote digestion and the absorption of animal to albumen; Black mold IFFI2447 and fungi aspergillus awamori IF-FI2194 are carbohydrase and amylase, can promote digestion and the absorption of animal to starch in the feed; Fungi Trichoderma viride AS3.3032 and AS3.2064 are cellulase, can promote and improve digestion and the decomposition of animal to fiber in the feed; Aspergillus niger IFFI2214 is a pectase, can promote and improve decomposition and the utilization of animal to colloid in the feed.Mineral matter charcoal acid calcium, calcium monohydrogen phosphate, the one, can be as carrier, the 2nd, can replenish the needs of animal to phosphorus and calcium; The interpolation of trace element then is the necessary composition of animal body itself, by external interpolation and rational consumption, can improve the balance of animal body growth key element greatly, improves the utilization rate of animal to feed, prevent disease and short long effect; The short long agent that adds can make animal reach and save food, and improves animal and increases, and accelerates animal and delivers for sale.
The production method of biologic inorganic composite feed additive for animals and fowls of the present invention has the following advantages and good effect:
1. adopt biology enzyme to become feed addictive, the complementation that the effect by biology enzyme can induced animal body endoenzyme, balance animal metabolic function, raising efficiency of feed utilization and remuneration with other trace element built;
2. production of enzyme preparation adopts solid fermentation, can reduce equipment investment, reduces production costs easy operating and production;
3. livestock and poultry biological active matter and growth factor, a large amount of biology enzymes and short long agent can promote the digestion of animal to feed, promote the growth of animal.
For introducing advantage of the present invention and effect in detail, quote some charts below and be elaborated:
Accompanying drawing is a process chart of the present invention
The product active ingredient table of table 1 for utilizing method of the present invention to make;
Table 2-5 is a product testing situation of utilizing method of the present invention to make.
The present invention utilizes the left U.S. aspergillus of biological fungi space, fungi aspergillus awamori, aspergillus niger, fungi Trichoderma viride to give birth to The complex enzyme formulation that produces is with reasonably mineral matter, trace element, multiannensional-sine, short long agent cooperate the production composite feed Feed additives.
Utilize biotechnology to prepare complex enzyme and reasonably mineral matter, trace element, multiannensional-sine, short long agent cooperate Become composite feed additive, reach after testing the good effect that all obtained on probation.
Used following biology enzyme and inorganic microelement, multiannensional-sine, short long agent among the present invention, wherein given birth to The thing bacterial classification needs from Chinese Academy of Sciences microorganism fungus kind center (Beijing) and Chinese microbiological industry microorganism center (food fermentation institute of former China National Light Industrial Products Department) buys mutagenesis and separates; Inorganic microelement and short long agent etc. are all bought from market Carry out preliminary treatment.
Divide below six steps to introduce respectively separation and the purifying of bacterial classification; The preparation of biology enzyme bacterial classification; Biology enzyme The preparation of preparation; The prescription of composite feed additive; The preliminary treatment of raw material; Production technology.
(1) separation of bacterial classification and purifying and mutagenesis
(1) culture medium
No. 1 agar medium of Gao Shi, meat extract peptone agar medium, the martin agar culture medium is contained 9ml The test tube of sterilized water is contained the 90ml sterilized water and with the conical flask of bead, aseptic glass is coated with rod, inoculation Ring, 10% phenol, sterile petri dish, streptomysin, soil sample etc.
(2) operating procedure
1. meat extract protein culture medium, No. 1 agar medium of Gao Shi, martin agar culture medium are dissolved, treat When being chilled to 55-60 °, add 10% phenol number droplet in No. 1 agar medium of Gao Shi, in the Ma Dingshi culture medium Add streptomysin solution, make to contain the plain 30 μ g of the chain certain kind of berries in every milliliter of cultivation. Be down flat respectively then plate, every kind of cultivation Base is three wares, and its method is test tube or the conical flask of right hand-held containing culture medium, put the flame next door, and left hand is taken flat Ware and loosening test tube plug or bottle stopper are clamped with volar edge and little finger of toe, the third finger and to be extracted, if in vitro or three Culture medium in the flask of angle once can use up, and then pipe close or bottle stopper needn't be clipped in the finger. Test tube (bottle) mouth exists Sterilize on the flame, left hand covers culture dish and open a seam near flame then, pours rapidly culture medium into approximately 15ml shakes culture dish gently after adding a cover, culture medium is evenly distributed, and is flat on the desktop, after waiting to coagulate Dull and stereotyped. Also plate can be placed near the desktop the flame, left-handed finger and middle finger are clamped pipe close and are opened Culture dish, the culture medium that reinjects is made flat board after shaking up. Preferably flat board was put room temperature 2-3 days, or 37 ° C cultivated 24 hours, checked after no bacterium colony and ware cover evaporated condensation water to re-use.
2. preparing product dilution takes by weighing product 10g and puts and contain the 90ml sterilized water and with the triangle of bead In the flask, about 20 minutes of jolting makes product fully mix with water, and bacterium is disperseed. With an aseptic suction of 1ml Pipe is therefrom drawn 1ml liquid and is injected the test tube that fills the 9ml sterilized water, and pressure-vaccum three times makes abundant mixing. So After from then on draw 1ml in the test tube with a 1ml aseptic straw again and inject the examination that another fills the 9ml sterilized water In the pipe, make by that analogy 10-1、10
-2、10
-3、10
-4、10
-5、10
-6Various dilution soil or product Product solution.
3. coating writes 10 with marking pen respectively with three planar bottom surface of above-mentioned every kind of culture medium-4、
10
-5, and 10-6, three kinds of dilution factors, use then three 1ml aseptic straws respectively by 10-4、10
-5, and 10-6, draw 0.2ml in the three pipeclay earth dilutions and check the number to put into and finish writing dilution flat board, with aseptic Glass is coated with rod and is coated with lightly evenly in media surface.
4. cultivate No. 1 culture medium flat plate of Gao Shi and Ma Dingshi culture medium flat plate are inverted, in 28 ℃ of greenhouses Cultivate 3-5 day, meat extract peptone flat-plate inverted places 37 ℃ of greenhouses to cultivate 2-3 day.
5. choose the single bacterium colony that grow after bacterium will be cultivated respectively picking be inoculated into above-mentioned three kinds of culture mediums tiltedly On the face, put respectively in 28 ℃ and 37 ℃ of greenhouses and cultivate, treat that lawn grows after, check whether lawn simple, also can Whether be single microorganism with the inspection of microscope smear staining, if there are other miscellaneous bacterias to mix, will be again Separate, purifying, until obtain pure culture.
6. utilize the ultraviolet ray irradiation to carry out mutagenesis
Opened earlier the ultraviolet lamp preheating 15 minutes, and made light wave stable, then culture dish (diluted and connect bacterial classification) is put Darkroom or transfer room from fluorescent tube 25cm place, shine and opened simultaneously red light in 0.5-2 minute, will cultivate then Ware is wrapped with black cloth and is placed in the incubator, cultivates 24 hours under 28-32 ℃ of temperature, and it is vigorous to select growth Energetic bacterial strain inserts in the slant medium and cultivates the production bacterial classification, so repeatedly separates the mutagenesis high activity Bacterial strain.
(2) preparation of biology enzyme bacterial classification
1. the preparation of the left U.S. aspergillus IFFI2378 of fungi space, aspergillus niger IFFI2447, fungi Trichoderma viride AS3.3032 bacterial classification:
1) the left U.S. aspergillus IFFI2378 bacterial classification preparation of fungi space
(1) slant strains production is:
1. culture medium: 5 ° of Be wheat tooth juice, 1.5-2.0% agar.
2. pH value nature (being about 5.5)
③1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement.
5. cultivation temperature 28-30 ℃
6. incubation time is 72 hours
(2) secondary seed is cultivated (solid culture)
1. culture medium: beancake powder 60%, wheat bran 35%, corn flour 4%, NH4Cl0.5％，CaCl0.3％，
NaHPO
40.2％
2. pH value is about 5.2
③1.5kg/cm
2Sterilized 30 minutes
4. lower the temperature insert about 35 ℃ slant strains (slant strains with sterilized water insert with transfer needle or the shovel with spore Son is scraped mixing in the entry, admixes in the culture after the dilution to stir), an inclined-plane can connect the 100g training Support thing. Culture inserted carry out shallow-layer fermentation in koji tray or fermentation machine or the Cans, about 5 centimeters of thickness About, indoor humidity about 85%, about 25 ℃ of room temperatures, the product temperature keeps about 32 ℃.
5. incubation time is 72 hours.
2) aspergillus niger IFFI2447 bacterial classification preparation:
(1) slant strains is cultivated
1. culture medium
5 Baume degrees brewer's wort 100ml add agar 2g, or Czapek's medium are: sucrose 3g, NaNO
30.2g, MgSO
47H
20.05gK
2HPO
40.1g, KCI0.05g, FeSO
40.001g, water 100ml, agar 2g.
2. pH value is natural
3. 1.5kg/cm
2Sterilized 30 minutes, and put into the inclined-plane.
4. insert bacterial classification by sterile working
5. cultivation temperature 28-30 ℃
6. incubation time is about 96 hours
(2) solid seed culture
1. culture medium:
Wheat bran 80%, soya-bean cake 10%, corn flour 5% starch 3%, sugar 1%, NaNO
0.2%, MgSO
47H
2O0.05g, K
2HPO
40.1%, KCI0.05%, FeSO
40.01%
2. material-water ratio is: 1: 0.8-1
3. pH value 5-6
4. 1.5kg/cm
2Sterilized 30 minutes,
5. lower the temperature about 35 ℃, insert slant strains (slant strains is inserted test tube with sterilized water and with transfer needle or shovel spore scraped mixing in the entry, admixes in the compost after the dilution to stir), a slant strains can meet culture 100g.Compost is inserted koji tray or fermentation frame or Cans or shallow-layer fermentation, about about 5 centimeters of thickness, the product temperature remains on about 32 ℃, indoor temperature about 86%, about 25 ℃ of room temperatures.
6. incubation time is about 72 hours
7. cultivate 24 hours stir culture material once, stirred once in 10 hours more later on.
8. the processing of compost: compost inserts gas institute fluidized drying machine or the drying room oven dry is advisable with about 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
3) fungi Trichoderma viride AS3.3032 strain preparation
(1) slant strains is cultivated
1. culture medium:
Potato 20g, glucose 1g, agar 2g, distilled water 100ml
2. pH value about 6
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement.
5. cultivation temperature 28-30 ℃
6. incubation time is 90 hours
(2) secondary solid seed culture
1. culture medium
Wheat bran 40%, corn flour 10%, powder of straw 48%, urea 0.3%, ammonium sulfate 0.3%,
2. material-water ratio is: 1: 1-1.2
3. pH value about 6
4. 1.5kg/cm
2Sterilized 30 minutes, and cooled to about 35 ℃.
5. inserting slant strains (pour into to use in the slant tube by pin the bacterium spore to be scraped be dispersed in the sterilized water sterilized water) stirs.
6. inorganic nitrogen is dissolved in the sterilized water, admixes in the postvaccinal material, mix.
7. the compost that mixes being inserted fermentation frame or fermentation machine or shallow-layer or deep ventilation cultivates.
8. humidity (indoor) about 85%.
9. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
10. incubation time is 72 hours
cultivates and stirred once in 24 hours, stirs once every 24 hours later on.
The using and preserving of solid spawn: wet bacterial classification can directly insert in the fermentation materials and cultivate, and the access amount is 0.6%, and dry strain 0.4% also can low temperature drying be pulverized the back and added CaCO
310%, saltcake 3%, after starch 2% mixed, sealing placed dry low-temp storage.
(7) according to the production method of claim 1 or 3 described biologic inorganic composite feed additives, it is characterized in that above-mentioned:
1) the U.S. aspergillus IFFI2379 bacterial classification in a fungi space left side is produced:
(1) slant strains is cultivated:
1. culture medium: 5 ° of Be brewer's worts, 1.5-2.0% agar.
2. pH value nature (being about 5.5)
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, by changing bacterium operation requirement inoculation
5. cultivation temperature 28-30 ℃
6. incubation time is 72 hours
(2) secondary seed is cultivated (solid culture)
1. culture medium: soya-bean cake powder 60%, wheat bran 35%, corn flour 4%, NH
4Cl0.5%, CaCl0.3%, Na
2HPO
40.2%
2. pH value is about 5.2
3. 1.5kg/cm
2Sterilized 30 minutes
4. lower the temperature and insert slant strains (slant strains is inserted with transfer needle with sterilized water or spared spore is scraped mixing in the entry, admixes in the culture after the dilution to stir) about 35 ℃, an inclined-plane can insert the 100g culture.To cultivate material inserts in koji tray or fermentation frame or the Cans and carries out the shallow-layer fermentation, about 5 centimeters of thickness, indoor humidity about 85%, about 25 ℃ of room temperatures, about 32 ℃ of product temperature maintenances.
5. incubation time is 72 hours
2) fungi aspergillus awamori IFFI2194 bacterial classification is produced
(1) slant strains is cultivated
1. culture medium: starch agar
Soluble starch 0.6g, NaNO
30.2g, MgSO
47H
2O0.05g, K
2HPO
41g, KCl0.05g, FeSO
47H
2O0.001g asks fat 2g, distilled water 100ml.
Elder generation with a spot of water furnishing pasty state, pours soluble starch in the starch fluid with the distilled water that seethes with excitement again, and cold scarce back adds other composition quantitatively to 100ml, packing after dissolving.
2. pH value: about 5.5
3. 1.5kg/cm
2Sterilized 30 minutes, and put into the inclined-plane.
4. insert bacterial classification by sterile working
5. cultivation temperature is 28 ℃-30 ℃
6. incubation time: 72 hours
(2) solid seed culture:
1. culture medium:
Wheat bran 85%, corn flour 5%, soya-bean cake 5%, straw cake 5%, powder of straw 5%
2. material-water ratio is: 1: 0.8-1
3. pH value about 5.5
4. 1.5kg/cm
2Sterilized 30 minutes,
5. lower the temperature about 35 ℃, insert slant strains (slant strains is inserted test tube with transfer needle or even spore is scraped mixing in the entry with sterilized water, admixes in the culture after rare to stir), an inclined-plane can connect the 100g culture.
6. compost is inserted (5 centimeters) fermented and cultured in koji tray or fermentation frame or shallow-layer fermentation vat (5 centimeters) or the Cans.
7. temperature is about 28 ℃-30 ℃, about 32 ℃ of product temperature
8. humidity about 85%
9. about incubation time 72n
10. cultivate and stirred seed once in 24 hours, 10h stirs once more later on.
3) fungi Trichoderma viride AS3.2064 strain preparation:
(1) slant strains is cultivated
1. culture medium:
Potato 20g, glucose 1g, distilled water 100ml.
2. pH value about 6
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement.
5. cultivation temperature 28-30 ℃
6. incubation time is 90 hours
(2) secondary solid seed culture
1. culture medium:
Wheat bran 40%, corn flour 10%, powder of straw 48%, urea 0.3%, ammonium sulfate 0.3%,
2. material-water ratio is: 1: 1-1.2
3. pH value about 6
4. 1.5kg/cm
2Sterilized 30 minutes, and cooled to about 35 ℃.
5. insert the inclined-plane bacterium with sterilized water pour in the slant tube with connect pin the bacterium spore is scraped be dispersed in the sterilized water) stir.
6. inorganic nitrogen is dissolved in the sterilized water, admixes in the postvaccinal material, mix.
7. the compost that mixes is inserted the fermentation of fermentation frame or fermentation machine or shallow-layer or deep ventilation.
8. humidity (indoor) about 85%.
9. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
10. incubation time is 72 hours
cultivates and stirred once in 24 hours, stirs once every 10 hours later on.
The using and preserving of solid spawn: wet bacterial classification can directly insert in the fermentation materials and cultivate, and the access amount is 1.6%, and dry strain 1% also can low temperature drying be pulverized the back and added CaCO
310%, saltcake 3%, after starch 2% mixed, sealing placed dry low-temp storage.
2. the preparation of the U.S. aspergillus IFFI2379 in a fungi space left side, fungi aspergillus awamori IFFI2194 strain preparation, fungi Trichoderma viride AS3.2064 bacterial classification:
1) the U.S. aspergillus IFFI2379 bacterial classification in a fungi space left side is produced:
(1) slant strains is cultivated:
1. culture medium: 5 ° of Be brewer's worts, 1.5-2.0% agar.
2. pH value (being about 5.5)
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement
5. cultivation temperature 28-30 ℃
6. incubation time is 72 hours
(2) secondary seed is cultivated (solid culture)
1. culture medium: soya-bean cake powder 60%, wheat bran 35%, corn flour 4%, NH
4Cl0.5%, CaCl0.3%, Na
2HPO
40.2%
2. pH value is about 5.2
3. 1.5kg/cm
2Sterilized 30 minutes
4. lower the temperature and insert slant strains (slant strains is inserted with transfer needle with sterilized water spore is scraped mixing in the entry, admixes in the culture after the dilution to stir) about 35 ℃, an inclined-plane can connect the 100g culture.To cultivate material inserts in koji tray or fermentation frame or the Cans and carries out the shallow-layer fermentation, about 5 centimeters of thickness, indoor humidity about 85%, about 25 ℃ of room temperatures, about 32 ℃ of product temperature maintenances.
5. incubation time is 72 hours
2) fungi aspergillus awamori IFFI2194 bacterial classification is produced
(1) slant strains is cultivated
1. culture medium: starch agar
Soluble starch 0.6g, NaNO
30.2g, MgSO
47H
2O0.05g, K
2HPO
41g, KCl0.05g, FeSO
47H
2O0.001g asks fat 2g, distilled water 100ml.
Elder generation with a spot of water furnishing pasty state, pours soluble starch in the starch fluid with the distilled water that seethes with excitement again, and cold scarce back adds other composition quantitatively to 100ml, packing after dissolving.
2. pH value about 5.5
3. 1.5kg/cm
2Sterilized 30 minutes, and put into the inclined-plane.
4. insert bacterial classification by sterile working.
5. cultivation temperature is 28 ℃-30 ℃
6. incubation time: 72 hours
(2) solid seed culture:
1. culture medium:
Wheat bran 85%, corn flour 5%, soya-bean cake 5%, straw cake 5%, powder of straw 5%
2. material-water ratio is: 1: 0.8-1
3. pH value about 5.5
4. 1.5kg/cm
2Sterilized 30 minutes,
5. lower the temperature about 35 ℃, insert slant strains (slant strains is inserted test tube with transfer needle or even spore is scraped mixing in the entry with sterilized water, admixes in the culture after rare to stir), an inclined-plane can connect the 100g culture.
6. compost is inserted (5 centimeters) fermented and cultured in koji tray or fermentation frame or shallow-layer fermentation vat (5 centimeters) or the Cans.
7. temperature is about 28 ℃, about 32 ℃ of product temperature.
8. humidity about 85%
9. about incubation time 72n
10. cultivate and stirred seed once in 24 hours, 10h stirs once more later on.
3) fungi Trichoderma viride AS3.2064 bacterial classification is produced:
(1) slant strains is cultivated
1. culture medium:
Potato 20g, glucose 1g, distilled water 100ml.
2. pH value about 6
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement.
5. cultivation temperature 28-30 ℃
6. incubation time is 90 hours
(2) secondary solid seed culture
1. culture medium:
Wheat bran 40%, corn flour 10%, powder of straw 48%, urea 0.3%, ammonium sulfate 0.3%,
2. material-water ratio is: 1: 1-1.2
3. pH value about 6
4. 1.5kg/cm
2Sterilized 30 minutes, and cooled to about 35 ℃.
5. insert the inclined-plane bacterium sterilized water is poured in the slant tube, the bacterium spore is scraped to be dispersed in the sterilized water stir with connecing pin.
6. inorganic nitrogen is dissolved in the sterilized water, admixes in the postvaccinal material, mix.
7. the compost that mixes is inserted fermentation frame or fermentation machine or shallow-layer or deep ventilation fermented and cultured.
8. humidity (indoor) about 85%.
9. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
10. incubation time is 72 hours
cultivates and stirred once in 24 hours, stirs once every 10 hours later on.
The using and preserving of solid spawn: wet bacterial classification can directly insert in the fermentation materials and cultivate, and the access amount is 1.6%, and dry strain 1% also can low temperature drying be pulverized the back and added CaCO
310%, saltcake 3%, after starch 2% mixed, sealing placed dry low-temp storage.
3. the preparation of the U.S. aspergillus IFFI2378 in a fungi space left side, fungi aspergillus awamori IFFI2194, aspergillus niger IFFI2447 and IFFI2214 and fungi Trichoderma viride AS3.3032 bacterial classification:
1) the U.S. aspergillus IFFI2378 strain preparation in a fungi space left side
(1) slant strains is cultivated:
1. culture medium: 5 ° of Be brewer's worts, 1.5-2.0% agar.
2. pH value (being about 5.5)
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement.
5. cultivation temperature 28-30 ℃
6. incubation time is 72 hours
(2) secondary seed is cultivated (solid culture)
1. culture medium: soya-bean cake powder 60%, wheat bran 35%, corn flour 4%, NH
4Cl0.5%, CaCl0.3%, Na
2HPO
40.2%
2. pH value is about 5.2
3. 1.5kg/cm
2Sterilized 30 minutes
4. lower the temperature and insert slant strains (slant strains is inserted with transfer needle with sterilized water spore is scraped mixing in the entry, admixes in the culture after the dilution to stir) about 35 ℃, an inclined-plane can connect the 100g culture.To cultivate material inserts in koji tray or fermentation frame or the Cans and carries out the shallow-layer fermentation, about 5 centimeters of thickness, indoor humidity about 85%, about 25 ℃ of room temperatures, about 32 ℃ of product temperature maintenances.
5. incubation time is 72 hours
6. cultivate 24 hours stir culture material once, stirred once in 10 hours more later on.
7. the processing of compost: product inserts pneumatic drier or the drying room oven dry is advisable with about 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
2) fungi aspergillus awamori IFFI2194 strain preparation
(1) slant strains is cultivated
1. culture medium: starch agar
Soluble starch 0.6g, NaNO
30.2g, MgSO
47H
2O0.05g, K
2HPO
41g, KCl0.05g, FeSO
47H
2O0.01g,
Agar 2g, distilled water 100ml.
Elder generation with a spot of water furnishing pasty state, pours soluble starch in the starch fluid with the distilled water that seethes with excitement again, and cold scarce back adds other composition quantitatively to 100ml, packing after dissolving.
2. pH value about 5.5
3. 1.5kg/cm
2Sterilized 30 minutes, and put into the inclined-plane.
4. insert bacterial classification by sterile working
5. cultivation temperature is 28 ℃-30 ℃
6. incubation time: 72 hours
(2) solid seed culture:
1. culture medium:
Wheat wheat bran 85%, corn flour 5%, soya-bean cake 5%, powder of straw 5%
2. material-water ratio is: 1: 0.8-1
3. pH value about 5.5
4. 1.5kg/cm
2Sterilized 30 minutes
5. lower the temperature about 35 ℃, insert slant strains (slant strains is inserted test tube with sterilized water and with transfer needle or shovel spore scraped mixing in the entry, admixes in the culture after the dilution to stir), an inclined-plane can connect the 100g culture.
6. compost is inserted (5 centimeters) fermented and cultured in koji tray or fermentation frame or shallow-layer fermentation vat (5 centimeters) or the Cans.
7. cultivation temperature is about 28 ℃, about 32 ℃ of product temperature
8. humidity about 85%
9. about incubation time 72h
10. cultivate and stirred seed once in 24 hours, 10h stirs once more later on.
Cultured bacterial classification can be inserted oven dry in dryer or the drying room, pulverizes the 30-40 order, adds CaCO
315%, saltcake 3%, starch 2% mix the back and store standby.
3) aspergillus niger IFFI2447 strain preparation
(1) slant strains is cultivated
1. culture medium
5 Baume degrees brewer's wort 100ml add agar 2g, or Czapek's medium are: sucrose 3g, NaNO
30.2g, MgSO
47H
2O0.05gK
2HPO
40.1g, KCI0.05g, FeSO
40.001g, water 100ml, agar 2g
2. pH value 5-6
3. 1.5kg/cm
2Sterilized 30 minutes, and put into the inclined-plane.
4. insert bacterial classification by sterile working
5. cultivation temperature 28-30 ℃
6. incubation time is about 96 hours
(2) solid seed culture
1. culture medium:
Wheat wheat bran 80%, soya-bean cake 10%, corn flour 5% starch 3%, sugar 1%, NaNO
30.2%MgSO
47H
2O0.05g, K
2HPO
40.1%, KCI0.05%, FeSO
40.01%
2. material-water ratio is: 1: 0.8-1
3. pH value 5-6
4. 1.5kg/cm
2Sterilized 30 minutes,
5. lower the temperature about 35 ℃, insert slant strains (slant strains is inserted test tube with sterilized water and with transfer needle or shovel spore scraped mixing in the entry, admixes in the compost after the dilution to stir), a slant strains can meet culture 100g.Compost is inserted koji tray or fermentation frame or Cans or shallow-layer fermentation, about about 5 centimeters of thickness, the product temperature remains on about 32 ℃, indoor temperature about 85%, about 25 ℃ of room temperatures.
6. incubation time is about 72 hours, and humidity about 85% is cultivated 24h and stirred once, and later every 10h stirs once.
7. cultured bacterial classification can be inserted oven dry in dryer or the drying room, pulverizes the 30-40 order, adds CaCO
315%, saltcake 3%, starch 2% mix the back and store standby.
4) preparation of aspergillus niger IFFI2214 bacterial classification
(1) slant strains is cultivated:
1. 5 ° of Be brewer's worts of culture medium, 1.5-2% agar.
2. pH value about 5.5
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement.
5. cultivation temperature 28-30 ℃
(2) secondary solid seed culture
1. culture medium:
Soya-bean cake powder 10%, wheat wheat bran 82%, corn flour 5%, pomace 2% (NH) SO
40.5%
2. material-water ratio is: 1: 0.8-1
3. pH value is natural
4. 1.5kg/cm
2Sterilized 30 minutes, and lowered the temperature about 35 ℃.
5. inserting slant strains (pour in the slant tube with transfer needle the bacterium spore to be scraped be dispersed in the sterilized water sterilized water) stirs.
6. admix in the compost after inorganic nitrogen being diluted with sterilized water.
7. the compost that mixes be will inoculate and fermentation frame or shallow-layer fermentation vat or deep ventilation fermentation vat or fermentation machine fermented and cultured, (in order to reduce pollution, the most handy Cans or tub) inserted.
8. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
9. humidity (indoor) about 85%
10. incubation time is 72 hours
cultivates and stirred once in 24 hours, stirs once every 24 hours later on.
The using and storing of solid spawn.Wet bacterial classification can directly insert and carry out wet bacterial classification 1.6% of solid fermentation access or dry strain 1% in the compost.Also can pulverize adding CaCO by low temperature drying
315%, saltcake 3%, starch 2% sealing places dry low-temp storage.
5) fungi Trichoderma viride AS3.3032 strain preparation
(1) slant strains is cultivated
1. culture medium:
Potato 20g, glucose 1g, agar 2g, distilled water 100ml.
2. pH value about 6
3. 1.5kg/cm
2Sterilized 30 minutes
4. put into the inclined-plane, inoculate by the sterile working requirement.
5. cultivation temperature 28-30 ℃
6. incubation time is 90 hours
(2) secondary solid seed culture
1. culture medium
Wheat bran 40%, corn flour 10%, powder of straw 48%, urea 0.3%, ammonium sulfate 0.3%,
2. material-water ratio is: 1: 1-1.2
3. pH value about 6
4. 1.5kg/cm
2Sterilized 30 minutes, and cooled to about 35 ℃.
5. inserting slant strains (pour in the slant tube with transfer needle the bacterium spore to be scraped be dispersed in the sterilized water sterilized water) stirs.
6. inorganic nitrogen is dissolved in the sterilized water, admixes in the postvaccinal material, mix.
7. the compost that mixes being inserted fermentation frame or fermentation machine or shallow-layer or deep ventilation cultivates.
8. humidity (indoor) about 85%.
9. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
10. incubation time is 72 hours
cultivates and stirred once in 24 hours, stirs once every 10 hours later on.
The using and preserving of solid spawn: wet bacterial classification can directly insert in the fermentation materials and cultivate, and the access amount is 1.6%, and dry strain 1% also can low temperature drying be pulverized the back and added CaCO
310%, saltcake 3%, after starch 2% mixed, sealing placed dry low-temp storage.
(3) the preparation production of biological enzyme formulation
1. the preparation of a fungi space left side U.S. aspergillus IFFI2378, aspergillus niger IFFI2447, Trichoderma viride AS3.3032 enzyme preparation
1) the U.S. aspergillus IFFI2378 product solid culture in a fungi space left side
1. culture medium: soya-bean cake powder 60%, wheat bran 35%, corn flour 4%, NH
4Cl0.5%, CaCl0.3%, Na
2HPO
40.2%
2. pH value is about 5.2
3. 1.5kg/cm
2Sterilized 30 minutes
4. lower the temperature and insert second class inoculum about 35 ℃ by 1% access bacterial classification sterilized water mixing, admix in the culture after the dilution and stir), to cultivate that material is inserted koji tray or the fermentation frame carries out the fermentation of shallow room, about about 5 centimeters of thickness, about indoor humidity 85%, about 25 ℃ of room temperatures, the product temperature keeps about 32 ℃.
5. incubation time is about 48 hours
6. material-water ratio is: 1: 0.7-0.9
7. cultivate 24h and stir once, later 10h stirs once.
8. the processing of fermentate:
Fermentate inserts dryer or the drying room oven dry is advisable with about 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
2) aspergillus niger IFFI2447 product solid culture
1. culture medium:
Wheat bran 80%, soya-bean cake 10%, corn flour 5% starch 3%, sugar 1%, NaNO
30.2%MgSO
47HO
20.05g, K
2HPO
40.1%, KCI0.05%, FeSO
40.01%
2. material-water ratio is: 1: 0.8-1
③PH?5-6
4. 1.5kg/cm
2Sterilized 30 minutes,
5. lower the temperature about 35 ℃, the access second class inoculum inserts by 1% and uses the sterilized water mixing, admixes in the compost after the dilution to stir).Compost is inserted koji tray or fermentation frame or shallow-layer fermentation, about about 5 centimeters of thickness, the product temperature remains on about 32 ℃, indoor temperature about 85%, about 25 ℃ of room temperatures.
6. incubation time is about 48 hours
7. cultivate and stirred once in 24 hours, stirred once in 10 hours more later on.
8. the processing of product: product inserts gas institute fluidized drying machine or the drying room oven dry is advisable with about 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
3) fungi Trichoderma viride AS3.3032 product solid culture
1. culture medium:
Wheat bran 30%, corn flour 10%, powder of straw 59%, urea 0.3%, ammonium sulfate 0.3%,
2. material-water ratio is: 1: 1-1.2%
3. pH value about 6
4. 1.5kg/cm
2Sterilized 30 minutes, and cooled to about 35 ℃.
5. inserting second class inoculum (being dispersed in the sterilized water by 1% access) stirs.
6. inorganic nitrogen is dissolved in the sterilized water, admixes in the postvaccinal material, mix.
7. the compost that mixes being inserted fermentation frame or fermentation machine or shallow-layer or deep ventilation cultivates.
8. humidity (indoor) about 85%.
9. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
10. incubation time is 48 hours
cultivates and stirred once in 24 hours, stirs once every 10 hours later on.
The using and preserving of solid spawn: wet bacterial classification can directly insert in the fermentation materials and cultivate, and the access amount is 1.6%, and dry strain 1% also can low temperature drying be pulverized the back and added CaCO
310%, saltcake 3%, after starch 2% mixed, sealing placed dry low-temp storage.
2. the preparation of a fungi space left side U.S. aspergillus IFFI2379, fungi aspergillus awamori IFFI2194, fungi Trichoderma viride AS3.2064 enzyme preparation:
1) the U.S. aspergillus IFFI2379 product solid culture in a fungi space left side
1. culture medium: soya-bean cake powder 60%, wheat bran 35%, corn flour 4%, NHcl0.5%, CaCl0.3%, NaHPO
0.2%
2. pH value is about 5.5
3. 1.5kg/cm
2Sterilized 30 minutes
4. lowering the temperature, the access second class inoculum inserts bacterial classification sterilized water mixing by 1% about 35 ℃, admixes in the culture after the dilution to stir.To cultivate that material is inserted koji tray or the fermentation frame carries out shallow-layer fermentation, about about 5 centimeters of thickness, indoor humidity about 85%, about 25 ℃ of room temperatures, about 32 ℃ of product temperature maintenances.
5. incubation time is 48 hours
6. material-water ratio is: 1: 0.7-0.9
7. cultivate 24h and stir once, later 10h stirs once.
8. the processing of fermentate:
Fermentate is inserted dryer or drying room or local method oven dry and is advisable with about 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
2) fungi aspergillus awamori IFFI2194 product solid culture
1. culture medium: wheat bran 85%, corn flour 5%, soya-bean cake 5%, powder of straw 5%
2. pH value about 5.5
3. material-water ratio is: 1: 0.8-1
4. 1.5kg/cm
2Sterilized 30 minutes, and lowered the temperature about 35 ℃.
5. insert wet bacterial classification 1.6% or the activation of dry strain 1% usefulness sterilized water 2 hours, access is cultivated in the material and is stirred.
6. compost being inserted fermentation frame or shallow-layer fermentation (5 centimeters) or deep ventilation fermentation (30-40cm) cultivates.
7. cultivation temperature (30-32 ℃ of product temperature)
8. humidity (indoor) about 85%
9. incubation time is about 72 hours
10. cultivate 24 hours stir culture material once, stirring in 10 hours turns once more later on.
The processing of product: product inserts airflow dryer or the drying room oven dry is advisable with about 40 ℃, pulverizes the 30-40 order, 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
3) fungi Trichoderma viride AS3.2064 product solid culture
1. culture medium:
Wheat bran 30%, corn flour 10%, powder of straw 59%, urea 0.3%, ammonium sulfate 0.3%
2. inserting wet bacterial classification 1.6% directly admixes in the compost or dried bacterium 1% usefulness sterilized water activation 2 hours inserts in the compost for about 35 ℃.
3. material-water ratio is: 1: 1-1.2
4. pH value about 6
5. 1.5kg/cm
2Sterilized 30 minutes, and cooled to about 35 ℃.
6. inorganic nitrogen is dissolved in the sterilized water, admixes in the postvaccinal material, mix.
7. the compost that mixes being inserted fermentation frame or fermentation machine or shallow-layer or deep ventilation cultivates.
8. humidity (indoor) about 85%.
9. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
10. incubation time is 72 hours
cultivates and stirred once in 24 hours, stirs once every 10 hours later on.
The processing of product: product is inserted the oven dry of pneumatic drier or drying room be advisable, add 15%CaCO with 40 ℃
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
3. the production method of the fungi space U.S. aspergillus IFFI2378 in a left side and fungi aspergillus awamori IFFI2194 and aspergillus niger IF-FI2447 and aspergillus niger IFFI2214 and fungi Trichoderma viride AS3.3032 enzyme preparation product is:
1) the U.S. aspergillus IFFI2378 product solid culture in a fungi space left side
1. culture medium: soya-bean cake powder 60%, wheat bran 35%, corn flour 4%NH
4Cl0.5%, CaCl0.3%, Na
2HPO
40.2%
2. pH value is about 5.2
3. 1.5kg/cm
2Sterilized 30 minutes
4. material-water ratio is: 1: 0.7-0.9
5. the wet bacterial classification 1.6% of access is directly admixed or dry strain 1%, activates about 2 hours (about 35 ℃ of temperature) with sterilized water, admixes in the compost to stir, and inserts koji tray or fermentation frame or shallow-layer or interior cultivation of deep ventilation fermentation machine.
6. about cultivation temperature 30-32 ℃, incubation time is about 48 hours.
7. humidity about 85%
8. cultivate 24h and stir once, later 10h stirs once.
9. the processing of fermentate:
Fermentate is inserted dryer or drying room or local method oven dry and is advisable with about 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
2) fungi aspergillus awamori IFFI2194 product solid culture
1. culture medium: wheat bran 85%, corn flour 5%, soya-bean cake 5%, powder of straw 5%
2. pH value about 5.5
3. material-water ratio is: 1: 0.8-1
4. 1.5kg/cm
2Sterilize and lowered the temperature about 35 ℃ in 30 minutes.
5. insert wet bacterial classification or the activation of dry strain 1% usefulness sterilized water 2 hours, access is cultivated in the material and is stirred.
6. compost being inserted fermentation frame or shallow-layer (5 centimeters) or deep ventilation fermentation (30-40cm) cultivates.
7. cultivation temperature (30-32 ℃ of product temperature)
8. humidity (indoor) about 85%
9. incubation time is about 72 hours
10. cultivate 24 hours stir culture material once, stirred once in 10 hours more later on.
10. the processing of product: product inserts airflow dryer or the drying room oven dry is advisable with about 40 ℃, adds 15%CaCO
3, stem nitre 0.5% is pulverized and is mixed the back package encapsulation.
3) aspergillus niger IFFI2447 product solid culture
1. culture medium
Wheat bran 80%, soya-bean cake 10%, corn flour 5% starch 3%, sugar 1%, NaNO
30.2%, MgSO
47H
2O0.05%, K
2HPO
40.1%, KCI0.05%, FeSO
40.01%
2. material-water ratio is: 1: 0.8-1
3. pH value 5-6
4. 1.5kg/cm
2Sterilized 30 minutes,
5. lower the temperature about 35 ℃, the access second class inoculum inserts by 1% and uses the sterilized water mixing, admixes in the compost after the dilution to stir.Compost is inserted koji tray or fermentation frame or shallow-layer fermentation, about about 5 centimeters of thickness, the product temperature remains on about 32 ℃, indoor temperature about 85%, about 25 ℃ of room temperatures.
6. incubation time is about 72 hours
7. cultivate 24 hours stir culture material once, stirred once in 10 hours more later on.
8. the processing of product: product inserts pneumatic drier or the drying room oven dry is advisable with about 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
4) aspergillus niger IFFI2214 product solid culture
1. culture medium:
Soya-bean cake powder 10%, wheat bran 82%, corn flour 5%, pomace 2% (NH) SO
40.5%
2. material-water ratio is: 1: 0.8-1
3. pH value is natural
4. 1.5kg/cm
2Sterilized 30 minutes, and lowered the temperature about 35 ℃.
5. inserting second class inoculum inserts to be dispersed in the sterilized water by 1% and stirs.
6. admix in the compost after inorganic nitrogen being diluted with sterilized water
7. the compost that mixes be will inoculate and fermentation frame or shallow-layer fermentation vat or deep ventilation or the fermentation of fermentation machine inserted.
8. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
9. humidity (indoor) about 85%
10. cultivated 48 hours
cultivates and stirred once in 24 hours, stirs once every 10 hours later on.
product treatment: product is inserted the oven dry of pneumatic drier or drying room be advisable, add 15%CaCO with 40 ℃
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
5) fungi Trichoderma viride AS3.3032 product solid culture
1. culture medium:
Wheat bran 30%, corn flour 10%, powder of straw 59%, urea 0.3%, ammonium sulfate 0.3%
2. inserting wet bacterial classification 1.6% directly admixes in the compost or dried bacterium 1% usefulness sterilized water activation 2 hours inserts in the composts for about 35 ℃ and stirs.
3. material-water ratio is: 1: 1-1.2
4. pH value about 6
5. 1.5kg/cm
2Sterilized 30 minutes, and cooled to about 35 ℃.
6. inorganic nitrogen is dissolved in the sterilized water, admixes in the postvaccinal material, mix.
7. the compost that mixes being inserted fermentation frame or fermentation machine or shallow-layer or deep ventilation cultivates.
8. humidity (indoor) about 85%.
9. cultivation temperature is indoor about 25 ℃, about 30 ℃ of product temperature.
10. incubation time is 72 hours
cultivates and stirred once in 24 hours, stirs once every 10 hours later on.
The product treatment: product is inserted pneumatic drier or drying room oven dry, and temperature is advisable with 40 ℃, adds 15%CaCO
3, saltcake 0.5% is pulverized and is mixed the back package encapsulation.
(4) prescription of compound additive
1). fattening pig additive contains: the plain 0.6-1.4% of compound vitamin, ferrous sulfate 1.3-2%, manganese sulfate 0.2-0.5%, copper sulphate 1-2%, zinc sulfate 1.5-2%, KI 0.002-0.003%, cobalt chloride 0.007-0.01%, sodium selenite 0.002-0.003%, olaquindox 0.3-1.6%, boric acid 0.06-0.1%, molybdenum 0.03%, methionine 5%, lysine 5%, complex enzyme 10-15%, lightweight charcoal acid calcium 35-40%, calcium monohydrogen phosphate 40-50%.
2). dorking addition agent contains: the plain 0.8-1.3% of compound vitamin, ferrous sulfate 1.5-2.5%, manganese sulfate 0.8-1.1%, copper sulphate 0.8-1.1, zinc sulfate 1.5-2.5%, KI 0.005-0.007%, sodium selenite 0.002-0.003%, olaquindox 0.3%, molybdenum 0.3%, methionine 10%, lysine 5%, complex enzyme 10-15%, calcium monohydrogen phosphate 40-50%, lightweight charcoal acid calcium 29-35%
(5), the preliminary treatment of composite feed additive raw material
The preliminary treatment of 1 raw material:
1. ferrous sulfate, copper sulphate, manganese sulfate, zinc sulfate have very big difficulty because of containing the moisture content work in-process, therefore must handle earlier, mainly are treated to the master with drying, can adopt force drying, under the relatively poor situation of condition and equipment, 100 orders were pulverized in available daylight airing.
2. the preliminary treatment of KI, cobalt chloride.
Adopt the absorbent balancing method, with allusion quotation potassium and the weighing accurately respectively of cobalt chloride raw material, each is with 1 then: 15-1: 20 ratio is dissolved in the water, and is sprayed on according to 1: 100 ratio respectively on the absorbent such as stone flour to mix again, and then carries out drying, pulverizes 200 orders.
3. the preliminary treatment of sodium selenite
General employing will contain in the hot water of 81 ℃ of sodium selenite addings 50% or more, through making 10kg aqueous fusion liquid after the 6min dissolving (is that the 1kg raw material is dissolved in the 10kg water and then is sprayed on the chaff powder in the mixer, the chaff powder is crossed 200 orders and is sprayed on the chaff powder with 1: 100 ratio, mix the oven dry back and cross 200 orders), mix, make diluent, be mixed into the premix of selenium again with other raw material.
2. the selection of carrier and diluent
Carrier is generally selected stone flour, precipitated calcium carbonate, zeolite material, starch, wheat husband, corn flour etc., enlarges step by step with 3-8 ratio doubly.
3. the granularity of raw material
Trace element is general to be required by the 80-400 order, promptly grain is through below 0.17-0.05mm, the grinding particle size of trace elements such as iron, zinc, manganese, copper, magnesium should all be crushed to below 200 orders (0.076mm) by denier compositions such as 80 orders (0.3mm) cobalt, iodine selenium, in order to reach very thin particulate, need to use special mill to carry out fine grinding, just can reach required granularity as iodine, potassium, selenium with the ball mill fine grinding, cobalt chloride, copper, iron, manganese, zinc will use micron order levigate to the several microns suitable granularities of ability assurance, reach best mixture homogeneity.
(6) the compound additive technological process of production is:
(1) technological process (seeing Figure of description)
(2) description of the process:
1. be that carrier dilutes with 3 times ratio expansion with KI, cobalt chloride, selenous acid steel with calcium monohydrogen phosphate, calcium bicarbonate earlier, add the grease of 1% carrier amount earlier, mix and added trace element in 5 minutes then, mix stirring taking-up after 20 minutes, carry out secondary dilution again.
2. be that carrier dilutes with 3 times ratio expansion with trace elements such as iron, zinc, copper, manganese with calcium monohydrogen phosphate, charcoal acid calcium or corn flour, earlier carrier is added in the mixer, the grease that adds carrier amount 1%, mix and add above trace element after 5 minutes, mix after 20 minutes and take out, carry out secondary dilution again.
3. be carrier with 3 times ratio mixed diluting with other growth stimulator, multiannensional-sine etc. with charcoal acid calcium or corn flour, earlier carrier is added in the mixer, add the grease of carrier amount 1%, mixed 5 minutes, add mixing taking-ups in 20 minutes such as growth stimulator then, carry out mixed diluting again 2 times.
4. will be that carrier dilutes expansion with 3 times ratio with calcium monohydrogen phosphate or wheat husband, corn flour with last pre-mixing agent.The subdivision enzyme also takes secondary dilution to enlarge.
5. dilute and mix for three times, the pre-mixing agent merging that whole secondaries mix is weighed, the capacity that carrier adds with calcium monohydrogen phosphate or wheat husband, corn flour or fish meal, add 1% grease then, mixed 5 minutes, then whole secondary dilution agent are added mixer and mixed 20 minutes, be complete first pre-mixed additive of 1%.
The instantiation prescription:
Example 1:
(1) complex enzyme formula: fungi space U.S. aspergillus IFFI2378 solid pharmaceutical preparation 60% in a left side and aspergillus niger IF-FI2447 solid pharmaceutical preparation 20% and fungi Trichoderma viride AS3.3032 20% solid pharmaceutical preparation mix in mixer, make complex enzyme, add in the composite feed additive.
(2) prescription of composite feed additive (growing and fattening pigs) is:
Above-mentioned complex enzyme is 15%, compound vitamin element 1.2%, ferrous sulfate 16%, manganese sulfate 0.3%, copper sulphate 1.4%, zinc sulfate 1.5%, KI 0.0025%, cobalt chloride 0.009%, sodium selenite 0.0025%, olaquindox 1%, boric acid 0.08%, methionine 5%, lysine 5%, lightweight charcoal acid calcium 35%, calcium monohydrogen phosphate is about about 35%, and more than prescription proposes the raw material pretreatment process operation according to the present invention and the process route and the explanation that provide are produced, can produce 1% composite feed additive, this example additive is mainly used in the fattening pannage additive, in 1% adding feed, can use through abundant mixing.
Example 2:
(1) complex enzyme formula: the fungi space is at U.S. aspergillus IFFI2379 solid pharmaceutical preparation 60%, fungi aspergillus awamori IFFI solid pharmaceutical preparation 20%, fungi Trichoderma viride AS3.2064 solid pharmaceutical preparation 20%, more than three kinds of enzyme preparations add in the mixer according to quantity and stir, make complex enzyme, add in the composite feed additive.
(2) prescription of composite feed additive (fryer) is:
Above-mentioned complex enzyme is 15%, compound vitamin element 1.2%, ferrous sulfate 1.8%, manganese sulfate 0.9%, copper sulphate 1%, zinc sulfate 1.5%, KI 0.006%, sodium selenite 0.002%, olaquindox 0.3%, molybdenum 0.3%, methionine 10%, lysine 5%, calcium monohydrogen phosphate 40%, lightweight charcoal acid calcium about 25%.More than prescription is produced according to the raw material pretreatment process operation of the present invention's proposition and process route and the explanation that provides, can produce 1% composite feed additive, this example additive is mainly used in the broiler fodder additive, in 1% adding feed, can use through abundant mixing.
Example 3:
(1) complex enzyme formula: the U.S. aspergillus IFFI2378 solid pharmaceutical preparation 30% in a fungi space left side, fungi aspergillus awamori IFFI2194 solid pharmaceutical preparation 20%, aspergillus niger IFFI2447 solid pharmaceutical preparation 10%, aspergillus niger IFFI2214 solid pharmaceutical preparation 10%, fungi Trichoderma viride AS3.3032 solid pharmaceutical preparation 30%, more than several solid pharmaceutical preparations add in the mixer according to quantity and stir, make complex enzyme, add in the feed addictive.
(2) prescription of composite feed additive: (sow)
Above-mentioned complex enzyme 15%, compound vitamin element 1.2%, ferrous sulfate 1.3%, manganese sulfate 0.2%, copper sulphate 1%, zinc sulfate 1.5%, KI 0.002%, cobalt chloride 0.007%, sodium selenite 0.002%, olaquindox 0.3%, boric acid 0.06%, molybdenum 0.03%, methionine 5%, lysine 5%, lightweight charcoal acid calcium 35%, about calcium monohydrogen phosphate 40%, more than prescription is produced according to the raw material pretreatment process operation of the present invention's proposition and process route and the explanation that provides, and can produce 1% composite feed additive, and the feed addictive of this case making is mainly used in the sow feed additive, in 1% adding feed, can use through abundant mixing.Table 1 is a composite feed additive sample analysis of components
Table 2
Illustrate: test 1 is that complete first compound additive and complex enzyme ratio are that 1: 0.15 (sample of the present invention) test 3 does not add any additives (seeing Table 2) for buy material essence (Tianjin rainbow) control group from market for complete first compound additive (buying from market) test 2
(1) complete first compound additive and enzyme-added 1: 1 pair of growth pig gaining effect
Table 3
Illustrate: test group is compared with control group, and daily gain is improved.Improve 18.6% in fattening stage test group 1, test group 2 improves 28.6%, and test group 3 improves 13%, sees table 3 for details.
The influence of complete first compound additive of table 4 and enzyme-added 1: 0.15 growing and fattening pigs meat feed ratio
Illustrate: test group is compared with control group, and the meat material all has decline.Descend 18% in fattening stage test group 1 meat feed ratio; Test group 2 descends 27%; Test group 3 descends 9%.See the Economic and Efficiency Analysis of table 4 table 5 for details
Claims (1)
1. the production method of a livestock and poultry biologic inorganic feed addictive comprises the preparation of biological multienzyme bacterium culture medium and the preparation of bacterial classification; The cultivation respectively of complex enzyme and the prescription and the technology of composite feed additive:
The batching of compound additive and being prepared as wherein:
The biology enzyme that cultivation and fermentation is conformed with quality requirement is dried and was cooperated the 20-30 order, with biological complex enzyme 10-15%, and micro-3-6%, vitamin 1.3-1.5%, amino acid 5-6%, short long agent 0.5-0.6%, mineral carrier is 70-85%;
The complex enzyme that adds in the compound additive is:
Fungi Aspergillus usamii IFFI2378 60-70%, aspergillus niger IFFI3032 20-30%, fungi Trichoderma viride AS3.2064 60-70%; Compatibility or
Fungi Aspergillus usamii IFFI2379 20%-30%, fungi aspergillus awamori IFFI2194 20-30%, IFFI2194 20-30%, 2064 60-70%; Compatibility or
Fungi Aspergillus usamii IFFI2379 30-40%, fungi aspergillus awamori IFFI2194 20-30%, aspergillus niger IFFI2447 10-20%, aspergillus niger IFFI2214 30-40%, fungi Trichoderma viride .AS3.3032 10-20%, compatibility;
More than the enzyme preparation and rational trace element of various compatibilities, cooperations such as multiannensional-sine, mineral matter, amino acid, growth stimulator are carried out preliminary treatment earlier according to specification requirement, then, mix again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97125739A CN1117524C (en) | 1997-12-30 | 1997-12-30 | Method for producing biologic inorganic composite feed additive for animals and fowls |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97125739A CN1117524C (en) | 1997-12-30 | 1997-12-30 | Method for producing biologic inorganic composite feed additive for animals and fowls |
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| Publication Number | Publication Date |
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| CN1187312A CN1187312A (en) | 1998-07-15 |
| CN1117524C true CN1117524C (en) | 2003-08-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN97125739A Expired - Fee Related CN1117524C (en) | 1997-12-30 | 1997-12-30 | Method for producing biologic inorganic composite feed additive for animals and fowls |
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| CN (1) | CN1117524C (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318582C (en) * | 2002-02-26 | 2007-05-30 | 东莞市豪发生物工程开发有限公司 | Once aspergillus niger fermentation process of obtaining complex enzyme of several kinds of enzyme and with high enzyme activity |
| CN100391357C (en) * | 2002-02-26 | 2008-06-04 | 东莞市豪发生物工程开发有限公司 | Bacilli fungi mixed fermentation for producing microbial formulation and compound enzyme feed additive |
| CN102178099B (en) * | 2011-04-26 | 2012-11-14 | 中国科学院东北地理与农业生态研究所 | Ruminant beet pulp-urea particle feed |
| CN102228135B (en) * | 2011-05-26 | 2013-02-13 | 浙江清华长三角研究院 | Core premix of green pig feed and production method |
| CN103125742A (en) * | 2011-11-21 | 2013-06-05 | 南通中科睿智科技服务有限公司 | Biological additive for livestock and poultry disease-prevention feed |
| CN103330057B (en) * | 2013-07-18 | 2014-07-09 | 广南(湛江)家丰饲料有限公司 | Composite feed supplement capable of effectively improving feed utilization ratio and preparation thereof |
| CN103740601B (en) * | 2013-12-23 | 2015-07-29 | 湖南鸿鹰生物科技有限公司 | A kind of aspergillus niger of high yield complex enzyme for feed and application thereof |
| CN104872406A (en) * | 2015-05-27 | 2015-09-02 | 柳州市亿廷贸易有限责任公司 | Poultry feed containing tricholoma lobayense heim mushroom bran |
| CN105670967A (en) * | 2016-03-04 | 2016-06-15 | 朝阳市德凤生态养殖有限责任公司 | Microecological ferment for fattening pig feeds and preparation method thereof, and active fermentation feed |
| CN106135733A (en) * | 2016-07-04 | 2016-11-23 | 昆明乐力升生物科技有限公司 | A kind of production method of compound premixed feed |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030608A (en) * | 1987-07-11 | 1989-01-25 | 广东省微生物研究所 | Preparation of bacterial protein by solid fermentation of mixed inoculated crude starch material and method for strain breeding thereof |
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1997
- 1997-12-30 CN CN97125739A patent/CN1117524C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030608A (en) * | 1987-07-11 | 1989-01-25 | 广东省微生物研究所 | Preparation of bacterial protein by solid fermentation of mixed inoculated crude starch material and method for strain breeding thereof |
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