CN103330057B - Composite feed supplement capable of effectively improving feed utilization ratio and preparation thereof - Google Patents
Composite feed supplement capable of effectively improving feed utilization ratio and preparation thereof Download PDFInfo
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Abstract
The invention discloses a composite feed supplement capable of effectively improving the feed utilization ratio and preparation thereof and belongs to the field of feed supplements. The feed supplement comprises the following raw materials in parts by weight: 20-30 parts of radix asparagi powder, 20-35 parts of echinacea powder, 15-30 parts of cattail pollen powder, 20-30 parts of dogwood powder, 1-5 part (s) of cordyceps taishanensis powder, 1-5 part (s) of cordyceps sinensis fermentation liquor extractive, 2-8 parts of bacillus natto agent, 5-10 parts of radix asparagi composite extractive, 2-4 parts of Aspergillus awamori culture and 0.5-2 part (s) of licorice powder. The raw materials are respectively prepared by a certain methods and are uniformly mixed to obtain the composite feed supplement. The composite feed supplement can effectively improve the immunity and intestinal health of pigs, reduce diseases of pigs, ensure healthiness of pigs, promote growth and development, and simultaneously protect the feed against mildew and nutrient substances against oxidation loss, thereby improving the feed utilization ratio.
Description
Technical field:
The present invention relates to a kind of pig's feed additive, particularly a kind of composite feed additive of effective raising efficiency of feed utilization relates to the preparation method of this additive simultaneously, belongs to feed additive field
Background technology:
Feed is the prime cost of animal-breeding, and animal-breeding cost has 60%-70% to come from feed cost use according to statistics, therefore reduces feed waste, improves efficiency of feed utilization, is the key point of carrying out aquaculture cost control and improving culture benefit.At present the common efficiency of feed utilization that exists of aquaculture is not high, and not exclusively, keeping is not good at causing feed moisture absorption to go mouldy or the problem such as infested in nutritional labeling digestion, and the existence of these problems has not only increased feeding cost greatly, and has caused the wasting of resources.How to address these problems, control aquaculture cost, reducing cultivation risk is the large bottleneck of one in aquaculture fast development.There is in recent years the much research about improving efficiency of feed utilization, use chemical synthesis additive agent field but mainly concentrate on, chemical synthesis additive can cause animal drug resistance, the problem such as residual, harm humans health, also has a large amount of excretas simultaneously, as the pernicious gas such as ammonia, hydrogen sulfide, contaminated environment.Chinese herbal feed additive is as the focus of emerging research, has the advantages such as natural, nutrition, side effect is little.Devoting Major Efforts To Developing Chinese herbal feed additive is to solving antibiotic residue problem, boost productivity, development stockbreeding industry, meet people's food security demand, dwindle China's animal husbandry and developed country's gap, strengthen the competitiveness of China's livestock products in international market, there is important economic implications and social benefit.Research shows, many Chinese herbal medicines itself just have good In Vitro Bacteriostasis ability, different from Western medicine is, Chinese herbal feed antibiotic health care agent noresidue, have no drug resistance, its mechanism of action is not only the inhibitory or killing effect to pathogenic microorganism, the more important thing is the adjustment to body disease-resistant repair ability, improve anti-stress ability, raising body's immunity and the Defense response function of body, thereby reach the object that improves breeding performonce fo animals and food utilization efficiency.
Chinese herbal medicine is some plants with given efficacy, these plants are each powerful, the effectiveness of for example Activities of Some Plants is as follows: lucid asparagus: its medicinal part is mainly piece root, there is replenishing the vital essence and removing heat, moisturize the effect of promoting the production of body fluid, modern pharmacology research shows that it has good curative effect to symptoms such as pulmonary tuberculosis, bronchitis, diphtheria, pertussis, dry mouth and throats, also has antibacterial immunity and anti-oxidation function simultaneously.Fructus Corni: its taste acid, puckery, tepor, returns liver kidney channel.Contain various active composition, comprise volatile ingredient, glucosides class and aglycon, tannin and organic acid etc.Fructus Corni water decoction can significantly raise mice serum hemolytic antibody and serum antibody IgG content.Carbohydrate in Fructus Corni has the effect of obvious Promote immunity reaction.Cattail pollen: be the dry pollen of Typhaceae plant raupo cattail, typha orientalis, theory of traditional Chinese medical science thinks that this property of medicine taste is sweet flat, returns liver, the heart, the spleen channel, effect of tool analgesia stagnation resolvation.Echinacea: originate in a class Echinacea plant in North America and Canada south, its main active is polysaccharide, alkylamide compound and Caffeic acids derivative.Research shows that different xylan and the arabinose in Echinacea can pass through to stimulate the activity of the lymphocytic propagation of monokaryon and macrophage, thereby has significant immunostimulation.Echinacea also has antibacterial and anti-inflammation functions, and its active component Cichoric acid has the immune anti-inflammatory properties of enhancing, can suppress hyaluronic acid, and protection collagen III is avoided degraded, and Escherichia coli are had to obvious inhibitory action.
How effectively to utilize existing herbal raw material preparation can effectively improve the feed addictive of animal immunizing power and the compound utilization ratio of raising feed, the high value utilization that realizes feed is one and is conducive to the project that feed industry develops in a healthy way.
Summary of the invention:
The object of the invention is to develop a kind of feed addictive that can effectively improve efficiency of feed utilization take Chinese herbal medicine as primary raw material, this feed addictive has that to improve the immunity, regulating intestinal canal of pig healthy and prevent going mouldy and the function of the oxidation such as vitamin of feed, thereby reduce the generation of disease, guarantee health, improve efficiency of feed utilization.
Technical scheme of the present invention is as follows:
Described feed addictive is made up of the raw material of following parts by weight:
Tianmen Green bean noodle 20-30 part; Echinacea powder 20-35 part; Cattail pollen powder 15-30 part; Fructus Corni powder 20-30 part; Cordyceps 1-5 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-8 part; Lucid asparagus composite extract 5-10 part; Aspergillus awamori culture 2-4 part; Licorice powder 0.5-2 part.
Described feed addictive is preferably made up of the raw material of following parts by weight:
Tianmen Green bean noodle 22-28 part; Echinacea powder 25-30 part; Cattail pollen powder 20-28 part; Fructus Corni powder 25-30 part; Cordyceps 1-4 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-6 part; Lucid asparagus composite extract 5-8 part; Aspergillus awamori culture 2-4 part; Licorice powder 1-2 part.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
Described Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
Described cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
Described Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
Described licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
Described cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Described cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70-85 ℃ and keeps after 1-2 hour, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract.
Described Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0; Percentage by weight.
Described bafillus natto bacterial preparation process:
Cultivate bafillus natto from inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in fermentation tank; control temperature is 19-24 ℃; ferment complete centrifugation of aerlbic culture 28-36 hour obtains wet thallus; add protective agent freeze drying and obtain microbial inoculum, protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, all the other are water.
Described lucid asparagus composite extract preparation method is as follows:
Take lucid asparagus 50-75 part; Echinacea powder 10-15 part; Fructus Corni powder 8-15 part; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, then in container, evenly mix and add the water of 3-6 times of weight, control 40 ℃-60 ℃ of temperature and keep 2-4h, increase the temperature to 80 ℃-85 ℃ and keep 3-5h, add the mixture of 2-3 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃-20 ℃ of maintenance 4-8h, filter; Filtrate Vacuum Concentration postlyophilization obtains lucid asparagus composite extract.The mass ratio of described butanols and propyl alcohol is 1:(0.3-0.6).Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 78-90%.
The preparation method of described aspergillus awamori culture is as follows:
Slant strains activation culture is transferred on slant medium and cultivates step by step and obtain after seed, seed is added to be equipped with in the fermentation vat of sterilising medium or pallet and mix rear cultivation, bent material cultivation temperature is controlled at 18-26 ℃, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through thermophilic digestion sterilization treatment, 121 ℃ of sterilising conditions control temperature, 1 hour time.
Drying and crushing: fermentation ends compost is dried on fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and crushing material particle diameter is more than 60 orders.
Culture medium composition: solid material parts by weight consist of: wheat bran 70, beancake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
The preparation method of feed addictive of the present invention is as follows:
According to raw material composition, Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed.
The addition of product of the present invention in live pig daily ration is 500-1000 gram/ton.
Beneficial effect:
The compatibility of above Chinese herbal medicine uses, and can effectively improve the immunity of pig and improve intestinal health, reduces the generation of pig disease, guarantee the health of pig, enhancing development, improves efficiency of feed utilization, and the while can prevent the oxidational losses of going mouldy of feed and nutriment.Use product of the present invention can make pig immunity improve a lot, antibiotic dosage reduces more than 60%, and pig goods taste and meat have obvious lifting, and organoleptic test evaluation shows to evaluate point raising more than 65%.
Technique scheme of the present invention has the following advantages compared to existing technology:
The present invention is composite by science, Chinese caterpillar fungus fermentation liquid extract, lucid asparagus composite extract, Chinese caterpillar fungus powder and bafillus natto preparation etc. have been realized to organic assembling, the traditional Chinese medicine ingredients and the bafillus natto preparation that there is natural antibacterial and improve immunity are particularly added, effectively reduce the use amount of antibiotics in letting animals feed, Cordyceps Militaris in cultivation process effectively absorption and accumulation the active component of lucid asparagus, improved the biological effect of product of the present invention.Lucid asparagus composite extract of the present invention containing polysaccharide, triterpene compound, glucoside, flavones, etc. bioactivator, safety non-toxic, there is antibacterial, antiviral, to strengthen immunity of organisms and the growth of promotion pig effect, can effectively improve food utilization efficiency, strengthen the immunity of pig, improve raise benefit, the security that can improve animal meat product simultaneously.
The specific embodiment
Below by specific embodiment narration product composition of the present invention and technique.Unless stated otherwise, in the present invention, technological means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope, the various changes that separation and Extraction condition in these embodiments is carried out or change also belong to protection scope of the present invention.
The preparation of bafillus natto microbial inoculum:
By in bafillus natto slant strains access shaking flask, cultivate step by step and obtain seed liquid, seed liquid is accessed to total measurement (volume) as 1.5 tons of secondary seed tanks take 10% inoculum concentration, 1 ton of fermentation medium loading amount, 24 ℃ of condition of culture cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 30 hours, cultivates and finishes cell concentration 5.0 × 10
8individual/ml.Centrifugation: adopt centrifuge separation and fermentation liquid to obtain thalline.After adding protective agent, adopt vacuum freeze-drying technique to process acquisition dry bacteria; Protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water.
Fermentation medium composition: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, all the other are pure water, pH6.8.
In described bafillus natto freeze-dried vaccine powder, number of viable is 2-8*10
8individual/g.
Bacterial classification used herein is: bafillus natto (Bacillus natto), strain number is CICC10023.
Cordyceps preparation method:
By in Cordyceps sinensis slant strains access shaking flask, cultivate step by step and obtain seed liquid, fermentation tank take 10% inoculum concentration access total measurement (volume) as 5 tons, 3 tons of fermentation medium loading amounts, 24 ℃ of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:0.4, tank pressure 0.05MPa, incubation time 72 hours.Zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, and fermented liquid is heated to 80 ℃ and keeps after 2 hours, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract.
In the present invention, Xu Jin hall volume " Chinese medicinal fungi " is shown in the cultivation of Cordyceps sinensis fungi liquid submerged fermentation liquid.
Slant medium consists of: percentage by weight, and potato (liquor) 20%, glucose 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, agar 2.0%, insufficient section pure water is supplied.
Seed culture medium composition is: percentage by weight, and sucrose 5%, glucose 2%, peptone 0.4%, soyabean protein powder 0.6%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Described Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0; Percentage by weight.
The present invention's Cordyceps sinensis used (Cordyceps sinensis) CICC50002 is purchased from Chinese industrial microorganism fungus kind preservation center.
Embodiment 1
Described feed addictive is preferably made up of the raw material of following parts by weight:
25 parts of Tianmen Green bean noodles; 27 parts, Echinacea powder; 25 parts, cattail pollen powder; 27 parts, Fructus Corni powder, 2 parts of cordyceps; 3 parts of Chinese caterpillar fungus fermentation liquid extracts; 4 parts of bafillus natto microbial inoculums, 6 parts of lucid asparagus composite extracts, 3 parts of aspergillus awamori cultures, 1.5 parts of licorice powders.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
Cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
Licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
Cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6%, Fructus Corni 1%, peptone 0.4%, soyabean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70 ℃ and keeps after 1.5 hours, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract.
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in fermentation tank; controlling temperature is 19 ℃; ferment for 32 hours complete centrifugation of aerlbic culture obtains wet thallus; add protective agent freeze drying and obtain microbial inoculum; protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water.
Described bacillus natto to ferment culture medium consists of: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8.
Preparation method is as follows for lucid asparagus composite extract:
Take 50 parts of lucid asparagus; 10 parts, Echinacea powder; 12 parts, Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, then in container, evenly mixes and add the water of 4 times of weight, controls temperature 50 C and keeps 3h, increase the temperature to 80 ℃ and keep 4h, add the mixture of 2 times of weight butanols of mixed material and propyl alcohol, control temperature to 16 ℃ and keep 6h, filter; Filtrate Vacuum Concentration postlyophilization obtains lucid asparagus composite extract.The mass ratio of described butanols and propyl alcohol is 1:0.3.Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 86%.
The preparation method of described aspergillus awamori culture is as follows:
Slant strains activation culture: aspergillus awamori slant strains is transferred on slant medium, cultivates 20 days for 20 ℃.
Solid first order seed is cultivated: picking aspergillus awamori slant strains accesses in 500 milliliters of triangular flasks that 100 grams of culture mediums are housed carries out seed culture, cultivates 3 days for 23 ℃.
Solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred as adding after fragment and in the triangular flask that sterilising medium is housed, carries out seed culture, condition of culture: cultivate 3 days for 22 ℃.
Solid fermentation is cultivated: secondary shake-flask seed is pulverized, added to be equipped with in the fermentation vat of sterilising medium or pallet to mix rear cultivation, bent material cultivation temperature is controlled at 22 ℃, humidity 80%, every 8 hours stirrings once, incubation time 6 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through thermophilic digestion sterilization treatment, 121 ℃ of sterilising conditions control temperature, 1 hour time.
Drying and crushing: fermentation ends compost is dried on fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and crushing material particle diameter is more than 60 orders.
Culture medium composition: solid material parts by weight consist of: wheat bran 70, beancake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
The preparation method of feed addictive of the present invention is as follows:
According to raw material composition, Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed to granulation.
The addition of this product in live pig daily ration is 800 grams/ton.
Embodiment 2
Described feed addictive is preferably made up of the raw material of following parts by weight:
22 parts of Tianmen Green bean noodles; 25 parts, Echinacea powder; 20 parts, cattail pollen powder; 25 parts, Fructus Corni powder, 1 part of cordyceps; 1 part of Chinese caterpillar fungus fermentation liquid extract; 2 parts of bafillus natto microbial inoculums, 5 parts of lucid asparagus composite extracts, 2 parts of aspergillus awamori cultures, 1 part of licorice powder.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
Cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
Licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
Cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6%, Fructus Corni 0.5%, peptone 0.4%, soyabean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 80 ℃ and keeps after 1.5 hours, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract.Experiment detects and shows, in the Chinese caterpillar fungus fermentation liquid extract obtaining after fermented liquid heat treated, polyoses content has improved 25-40% than the polyoses content of non-heat treated.
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in fermentation tank; controlling temperature is 19 ℃; ferment for 28 hours complete centrifugation of aerlbic culture obtains wet thallus; add protective agent freeze drying and obtain microbial inoculum, protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5 trehaloses.
Described bacillus natto to ferment culture medium consists of: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8.
Preparation method is as follows for lucid asparagus composite extract:
Take 50 parts of lucid asparagus; 10 parts, Echinacea powder; 8 parts, Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, then in container, evenly mixes and add the water of 3 times of weight, controls 40 ℃ of temperature and keeps 2h, increase the temperature to 80 ℃ and keep 3h, add the mixture of 2 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃ and keep 4h, filter; Filtrate Vacuum Concentration postlyophilization obtains lucid asparagus composite extract.The mass ratio of described butanols and propyl alcohol is 1:0.5.Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 80%.
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: aspergillus awamori slant strains is transferred on slant medium, cultivates 2 days for 18 ℃.
Solid first order seed is cultivated: picking aspergillus awamori slant strains accesses in 500 milliliters of triangular flasks that 100 grams of culture mediums are housed carries out seed culture, cultivates 3 days for 18 ℃.
Solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred as adding after fragment and in the triangular flask that sterilising medium is housed, carries out seed culture, condition of culture: cultivate 3 days for 18 ℃.
Solid fermentation is cultivated: secondary shake-flask seed is pulverized, added to be equipped with in the fermentation vat of sterilising medium or pallet to mix rear cultivation, bent material cultivation temperature is controlled at 18 ℃, humidity 82%, every 8 hours stirrings once, incubation time 5 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through thermophilic digestion sterilization treatment, 121 ℃ of sterilising conditions control temperature, 1 hour time.
Drying and crushing: fermentation ends compost is dried on fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and crushing material particle diameter is more than 60 orders.
Culture medium composition: solid material parts by weight consist of: wheat bran 70, beancake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
The preparation method of feed addictive of the present invention is as follows:
According to raw material composition, Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed to granulation.
The addition of this product in live pig daily ration is 500 grams/ton.
Embodiment 3
Described feed addictive is preferably made up of the raw material of following parts by weight:
28 parts of Tianmen Green bean noodles; 30 parts, Echinacea powder; 28 parts, cattail pollen powder; 30 parts, Fructus Corni powder, 4 parts of cordyceps; 5 parts of Chinese caterpillar fungus fermentation liquid extracts; 6 parts of bafillus natto microbial inoculums, 8 parts of lucid asparagus composite extracts, 4 parts of aspergillus awamori cultures, licorice powder 2%.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
Cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
Licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
Cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 1%, Fructus Corni 1%, peptone 0.4%, soyabean protein powder 1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 85 ℃ and keeps after 2 hours, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract.
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in fermentation tank; controlling temperature is 24 ℃; ferment for 36 hours complete centrifugation of aerlbic culture obtains wet thallus; add protective agent freeze drying and obtain microbial inoculum; protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water.
Described bacillus natto to ferment culture medium consists of: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8.
Preparation method is as follows for lucid asparagus composite extract:
Take 75 parts of lucid asparagus; 15 parts, Echinacea powder; 15 parts, Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, then in container, evenly mixes and add the water of 6 times of weight, controls temperature 60 C and keeps 4h, increase the temperature to 85 ℃ and keep 5h, add the mixture of 3 times of weight butanols of mixed material and propyl alcohol, control temperature to 20 ℃ and keep 8h, filter; Filtrate Vacuum Concentration postlyophilization obtains lucid asparagus composite extract.Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 90%.
The mass ratio of described butanols and propyl alcohol is 1:0.6.
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: aspergillus awamori slant strains is transferred on slant medium, cultivates 3 days for 25 ℃.
Solid first order seed is cultivated: picking aspergillus awamori slant strains accesses in 500 milliliters of triangular flasks that 100 grams of culture mediums are housed carries out seed culture, cultivates 3 days for 26 ℃.
Solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred as adding after fragment and in the triangular flask that sterilising medium is housed, carries out seed culture, condition of culture: cultivate 3 days for 26 ℃.
Solid fermentation is cultivated: secondary shake-flask seed is pulverized, added to be equipped with in the fermentation vat of sterilising medium or pallet to mix rear cultivation, bent material cultivation temperature is controlled at 26 ℃, humidity 85%, every 6 hours stirrings once, incubation time 8 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through thermophilic digestion sterilization treatment, 121 ℃ of sterilising conditions control temperature, 1 hour time.
Drying and crushing: fermentation ends compost is dried on fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and crushing material particle diameter is more than 60 orders.
Culture medium composition: solid material parts by weight consist of: wheat bran 70, beancake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
Aspergillus awamori (Aspergillus awamori) CICC2250 purchases from Chinese industrial microorganism fungus kind preservation administrative center.
The addition of this product in live pig daily ration is 1000 grams/ton.
Result of use experiment
The result of use test of the embodiment of the present invention 1 feed addictive in weanling pig:
It is 120 of the healthy sucking pigs of (11.16 ± 0.45) kg that experimental animal is selected Guangnan (Zhanjiang) subordinate plant of Jia Feng feed corporation,Ltd average weight, and statistical analysis body weight difference is not remarkable.Adopt single-factor Randomized Designs, by 120 healthy sucking pigs, by male and female half and half, be divided into 2 groups (control group and test group), every group of 6 repetitions, 10 pigs of each repetition.800 grams/ton of the products of the present invention that test group interpolation embodiment 1 makes, control group does not add product of the present invention.Feed continuously 25 days, compared with control group, use invention feed addictive can obtain following effect:
| Project | Test group | Control group | Deviation |
| Starting weight (kg) | 11.08 | 11.24 | —— |
| End heavy (kg) | 23.54 | 22.40 | 4.8% |
| Daily ingestion amount (g) | 758.6 | 750.1 | —— |
| Daily gain (g) | 498.2 | 446.5 | 10.4% |
| Feedstuff-meat ratio | 1.52 | 1.68 | -10.5% |
| Diarrhea rate (%) | 4.8 | 11.6% | -141.7% |
Result of the test shows: in sucking pig daily ration, add product of the present invention, can effectively improve the daily gain of sucking pig, reduce feedstuff-meat ratio, reduce diarrhoea simultaneously, the hair color of experiment pig is carried out to sensory evaluation scores, experimental group scoring reaches 8.9 points, and control group is 6.5 points; Above-mentioned data declaration product of the present invention can effectively improve the utilization rate of feed, improves the immunity of sucking pig simultaneously, reduces diarrhoea.
Claims (7)
1. effectively improve a composite feed additive for efficiency of feed utilization, comprise the raw material components of following parts by weight: Tianmen Green bean noodle 20-30 part; Echinacea powder 20-35 part; Cattail pollen powder 15-30 part; Fructus Corni powder 20-30 part; Cordyceps 1-5 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-8 part; Lucid asparagus composite extract 5-10 part; Aspergillus awamori culture 2-4 part; Licorice powder 0.5-2 part; Described cordyceps sinensis fermentation liquor method for preparing extractive is: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70-85 ℃ and keeps after 1-2 hour, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract; Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0; Percentage by weight; Described lucid asparagus composite extract preparation method is as follows: take lucid asparagus 50-75 part; Echinacea powder 10-15 part; Fructus Corni powder 8-15 part; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, then in container, evenly mix and add the water of 3-6 times of weight, control 40 ℃-60 ℃ of temperature and keep 2-4h, increase the temperature to 80 ℃-85 ℃ and keep 3-5h, add the mixture of 2-3 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃-20 ℃ of maintenance 4-8h, filter; Filtrate Vacuum Concentration postlyophilization obtains lucid asparagus composite extract;
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: aspergillus awamori slant strains is transferred on slant medium, cultivates 2-3 days for 18-25 ℃;
Solid first order seed is cultivated: picking aspergillus awamori slant strains accesses in 500 milliliters of triangular flasks that 100 grams of culture mediums are housed carries out seed culture, cultivates 3 days for 18-26 ℃;
Solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred as adding after fragment and in the triangular flask that sterilising medium is housed, carries out seed culture, condition of culture: 18-26 ℃ and cultivate 3 days;
Solid fermentation is cultivated: secondary shake-flask seed is pulverized, added to be equipped with in the fermentation vat of sterilising medium or pallet to mix rear cultivation, bent material cultivation temperature is controlled at 18-26 ℃, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through thermophilic digestion sterilization treatment, 121 ℃ of sterilising conditions control temperature, 1 hour time;
Drying and crushing: fermentation ends compost is dried on fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and crushing material particle diameter is more than 60 orders;
Culture medium composition: solid material parts by weight consist of: wheat bran 70, beancake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8, adds the running water with solid matter equivalent; Initial pH nature;
The preparation method of described bafillus natto microbial inoculum is as follows: cultivate bafillus natto from inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in fermentation tank; control temperature is 19-24 ℃; ferment complete centrifugation of aerlbic culture 28-36 hour obtains wet thallus; add protective agent freeze drying and obtain microbial inoculum, protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, all the other are water.
2. a composite feed additive for effective raising efficiency of feed utilization as claimed in claim 1, comprises the raw material components of following parts by weight: Tianmen Green bean noodle 22-28 part; Echinacea powder 25-30 part; Cattail pollen powder 20-28 part; Fructus Corni powder 25-30 part; Cordyceps 1-4 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-6 part; Lucid asparagus composite extract 5-8 part; Aspergillus awamori culture 2-4 part; Licorice powder 1-2 part.
3. a composite feed additive for effective raising efficiency of feed utilization as claimed in claim 1 or 2, is characterized in that, the mass ratio of described butanols and propyl alcohol is 1:0.3-0.6.
4. prepare the preparation method of the composite feed additive of effective raising efficiency of feed utilization as claimed in claim 1 or 2 for one kind:
According to raw material composition, Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed to granulation;
Cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains cordyceps; Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0; Percentage by weight;
Cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70-85 ℃ and keeps after 1-2 hour, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract; Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0; Percentage by weight;
Bafillus natto bacterial preparation process:
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and control temperature is 19-24 ℃, aerlbic culture 28-36 hour, and throughput is 2.0m
3/ minute; The complete centrifugation of fermenting obtains wet thallus, adds protective agent freeze drying and obtains microbial inoculum, and protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water;
Described lucid asparagus composite extract preparation method is as follows:
Take lucid asparagus 50-75 part; Echinacea powder 10-15 part; Fructus Corni powder 8-15 part; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, then in container, evenly mix and add the water of 3-6 times of weight, control 40 ℃-60 ℃ of temperature and keep 2-4h, increase the temperature to 80 ℃-85 ℃ and keep 3-5h, add the mixture of 2-3 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃-20 ℃ of maintenance 4-8h, filter; Filtrate Vacuum Concentration postlyophilization obtains lucid asparagus composite extract;
The mass ratio of described butanols and propyl alcohol is 1:0.3-06;
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: aspergillus awamori slant strains is transferred on slant medium, cultivates 2-3 days for 18-25 ℃;
Solid first order seed is cultivated: picking aspergillus awamori slant strains accesses in 500 milliliters of triangular flasks that 100 grams of culture mediums are housed carries out seed culture, cultivates 3 days for 18-26 ℃;
Solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred as adding after fragment and in the triangular flask that sterilising medium is housed, carries out seed culture, condition of culture: 18-26 ℃ and cultivate 3 days;
Solid fermentation is cultivated: secondary shake-flask seed is pulverized, added to be equipped with in the fermentation vat of sterilising medium or pallet to mix rear cultivation, bent material cultivation temperature is controlled at 18-26 ℃, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through thermophilic digestion sterilization treatment, 121 ℃ of sterilising conditions control temperature, 1 hour time;
Drying and crushing: fermentation ends compost is dried on fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and crushing material particle diameter is more than 60 orders;
Culture medium composition: solid material parts by weight consist of: wheat bran 70, beancake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
5. the preparation method of the composite feed additive of effective raising efficiency of feed utilization according to claim 4, described feed addictive is made up of the raw material of following parts by weight: 22 parts of Tianmen Green bean noodles; 25 parts, Echinacea powder; 20 parts, cattail pollen powder; 25 parts, Fructus Corni powder, 1 part of cordyceps; 1 part of Chinese caterpillar fungus fermentation liquid extract; 2 parts of bafillus natto microbial inoculums, 5 parts of lucid asparagus composite extracts, 2 parts of aspergillus awamori cultures, 1 part of licorice powder;
Preparation method is as follows:
According to raw material composition, Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed to granulation;
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6%, Fructus Corni 0.5%, peptone 0.4%, soyabean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0;
Cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 80 ℃ and keeps after 1.5 hours, and fermented liquid spray drying is pulverized and obtained Chinese caterpillar fungus fermentation liquid extract;
Bacillus natto to ferment culture medium consists of: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, all the other are pure water, pH6.8;
Preparation method is as follows for lucid asparagus composite extract:
Take 50 parts of lucid asparagus; 10 parts, Echinacea powder; 8 parts, Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, then in container, evenly mix and add the water of 3-6 times of weight, control 40 ℃ of temperature and keep 2h, increase the temperature to 80 ℃ and keep 3h, add the mixture of 2 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃ and keep 4h, filter; Filtrate Vacuum Concentration postlyophilization obtains lucid asparagus composite extract; The mass ratio of described butanols and propyl alcohol is 1:0.5;
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: aspergillus awamori slant strains is transferred on slant medium, cultivates 2 days for 18 ℃;
Solid first order seed is cultivated: picking aspergillus awamori slant strains accesses in 500 milliliters of triangular flasks that 100 grams of culture mediums are housed carries out seed culture, cultivates 3 days for 18 ℃;
Solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred as adding after fragment and in the triangular flask that sterilising medium is housed, carries out seed culture, condition of culture: cultivate 3 days for 18 ℃;
Solid fermentation is cultivated: secondary shake-flask seed is pulverized, added to be equipped with in the fermentation vat of sterilising medium or pallet to mix rear cultivation, bent material cultivation temperature is controlled at 18 ℃, humidity 82%, every 8 hours stirrings once, incubation time 5 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through thermophilic digestion sterilization treatment, 121 ℃ of sterilising conditions control temperature, 1 hour time;
Drying and crushing: fermentation ends compost is dried on fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and crushing material particle diameter is more than 60 orders.
6. according to the preparation method of the composite feed additive of the effective raising efficiency of feed utilization described in claim 4 or 5, aspergillus awamori culture medium consists of, solid material parts by weight consist of: wheat bran 70, beancake powder 6, Tianmen Green bean noodle 6, Fructus Corni powder 3, cornstarch 6, adds the running water with solid matter equivalent; Initial pH nature.
7. effectively improve as claimed in claim 1 or 2 the composite feed additive of efficiency of feed utilization in an application of preparing in live pig daily ration, the addition of the composite feed additive of described effective raising efficiency of feed utilization in live pig daily ration is 500-1000 gram/ton.
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| CN104905016A (en) * | 2014-03-10 | 2015-09-16 | 天津工业大学 | A feed additive and a preparation method thereof |
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| CN104621381A (en) * | 2014-12-25 | 2015-05-20 | 北京资源亚太饲料科技有限公司 | Compound feed with fermented cordyceps sinensis extract for weaned piglets |
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| CN106858039A (en) * | 2017-03-22 | 2017-06-20 | 福清双胞胎饲料有限公司 | A kind of green feed additive for effectively improving efficiency of feed utilization |
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| CN111567689A (en) * | 2020-05-18 | 2020-08-25 | 西北农林科技大学 | Pig feed for improving intestinal health and preparation method thereof |
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| CN112602824A (en) * | 2020-12-15 | 2021-04-06 | 青海珠峰冬虫夏草工程技术研究有限公司 | Feed additive containing hirsutella sinensis culture and preparation method thereof |
| CN112538436B (en) * | 2020-12-23 | 2021-08-17 | 江苏三仪生物工程有限公司 | Preparation method of acremonium terricola culture |
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